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Supplemental Information
Screen Description
c3_3
BCC Core Screens / c3_3
Created 2025-01-08.
Peggy: 3350 and 4000, diverse buffers, pH range and salts.
Export
c3_3
Barcodes
c3_3
- Z0002639
- Z0002640
Screen Structure
c3_3
Well Count | 96 |
---|---|
Number of distinct ingredients | 53 |
Internal diversity (score mean dist) | 0.854 |
Screen Composition
ingredient type | c3_3 |
---|---|
Buffer | 69 |
Organic (volatile) | 4 |
Polymer | 96 |
Salt | 81 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 80 μl of magnesium acetate BCC (2.5 M) 520 μl of water (100 v/v) |
A2 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 400 μl of water (100 v/v) |
A3 |
| NA | 200 μl of magnesium formate BCC (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
A4 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium nitrate BCC (1 M) 400 μl of water (100 v/v) |
A5 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium sulfate BCC (1 M) 400 μl of water (100 v/v) |
A6 |
| NA | 40 μl of potassium acetate BCC (5 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 560 μl of water (100 v/v) |
A7 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of potassium chloride BCC (1 M) 400 μl of water (100 v/v) |
A8 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of potassium dihydrogen phosphate BCC (1 M) 400 μl of water (100 v/v) |
A9 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of potassium fluoride BCC (1 M) 400 μl of water (100 v/v) |
A10 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of potassium formate BCC (1 M) 400 μl of water (100 v/v) |
A11 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 40 μl of potassium iodide BCC (5 M) 560 μl of water (100 v/v) |
A12 |
| NA | 200 μl of potassium nitrate BCC (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
B1 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of potassium sodium tartrate BCC (2 M) 500 μl of water (100 v/v) |
B2 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of potassium sulfate BCC (0.5 M) 200 μl of water (100 v/v) |
B3 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of potassium thiocyanate BCC (2 M) 500 μl of water (100 v/v) |
B4 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of sodium acetate BCC (1 M) 400 μl of water (100 v/v) |
B5 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium chloride BCC (2 M) 500 μl of water (100 v/v) |
B6 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of sodium dihydrogen phosphate BCC (1 M) 400 μl of water (100 v/v) |
B7 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of sodium fluoride BCC (1 M) 400 μl of water (100 v/v) |
B8 |
| NA | 50 μl of sodium formate BCC (4 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 550 μl of water (100 v/v) |
B9 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of sodium iodide BCC (1 M) 400 μl of water (100 v/v) |
B10 |
| pH 7 | 58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 541 μl of water (100 v/v) |
B11 |
| NA | 200 μl of sodium nitrate BCC (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
B12 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 166 μl of sodium sulfate BCC (1.2 M) 433 μl of water (100 v/v) |
C1 |
| NA | 100 μl of sodium thiocyanate BCC (2 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 500 μl of water (100 v/v) |
C2 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 80 μl of triammonium citrate BCC (2.5 M) 520 μl of water (100 v/v) |
C3 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of trilithium citrate BCC (1 M) 400 μl of water (100 v/v) |
C4 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of tripotassium citrate BCC (1 M) 400 μl of water (100 v/v) |
C5 |
| NA | 125 μl of trisodium citrate BCC (1.6 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 475 μl of water (100 v/v) |
C6 |
| NA | 20 μl of zinc acetate BCC (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 580 μl of water (100 v/v) |
C7 |
| pH 6.5 | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 500 μl of water (100 v/v) |
C8 |
| pH 7 | 127 μl of sodium MOPS BCC pH 6.2 (0.5 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of magnesium chloride BCC (1 M) 72 μl of sodium MOPS BCC pH 8.2 (0.5 M) 300 μl of water (100 v/v) |
C9 |
| pH 3.5 | 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of water (100 v/v) |
C10 |
| pH 4.5 | 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 400 μl of water (100 v/v) |
C11 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 400 μl of water (100 v/v) |
C12 |
| pH 5.5 | 40 μl of ammonium acetate BCC (5 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 360 μl of water (100 v/v) |
D1 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
D2 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
D3 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of water (100 v/v) |
D4 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium chloride BCC (2 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 300 μl of water (100 v/v) |
D5 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
D6 |
| pH 6.5 | 40 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
D8 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
D9 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 200 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium chloride BCC (2 M) 300 μl of water (100 v/v) |
D11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
D12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 40 μl of ammonium acetate BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
E1 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
E2 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
E3 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 200 μl of water (100 v/v) |
E4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium chloride BCC (2 M) 300 μl of water (100 v/v) |
E5 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
E6 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 40 μl of ammonium acetate BCC (5 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
E7 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
E8 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
E9 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 200 μl of water (100 v/v) |
E10 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium chloride BCC (2 M) 300 μl of water (100 v/v) |
E11 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 160 μl of polyethylene glycol 4000 BCC (50 w/v) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 740 μl of water (100 v/v) |
E12 |
| pH 6 | 61 μl of sodium MES BCC pH 5.2 (1 M) 160 μl of polyethylene glycol 4000 BCC (50 w/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 740 μl of water (100 v/v) |
F1 |
| pH 6 | 136 μl of DL-malate-imidazole BCC pH 9 (1 M) 160 μl of polyethylene glycol 4000 BCC (50 w/v) 64 μl of DL-malate-imidazole BCC pH 2 (1 M) 640 μl of water (100 v/v) |
F2 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 200 μl of polyethylene glycol 4000 BCC (50 w/v) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 700 μl of water (100 v/v) |
F3 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 200 μl of polyethylene glycol 4000 BCC (50 w/v) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 500 μl of sodium chloride BCC (2 M) 200 μl of water (100 v/v) |
F4 |
| pH 7 | 160 μl of DL-malate-imidazole BCC pH 9 (1 M) 200 μl of polyethylene glycol 4000 BCC (50 w/v) 40 μl of DL-malate-imidazole BCC pH 2 (1 M) 600 μl of water (100 v/v) |
F5 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 240 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 660 μl of water (100 v/v) |
F6 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 240 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 100 μl of ammonium sulfate BCC (1 M) 560 μl of water (100 v/v) |
F7 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 1 μl of dipotassium tartrate BCC (3 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 598 μl of water (100 v/v) |
F8 |
| pH 5.5 | 40 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 200 μl of potassium bromide BCC (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 450 μl of water (100 v/v) |
F9 |
| pH 6 | 136 μl of DL-malate-imidazole BCC pH 9 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 100 μl of magnesium chloride BCC (1 M) 64 μl of DL-malate-imidazole BCC pH 2 (1 M) 400 μl of water (100 v/v) |
F10 |
| pH 6 | 300 μl of polyethylene glycol 4000 BCC (50 w/v) 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 600 μl of water (100 v/v) |
F11 |
| pH 6 | 300 μl of polyethylene glycol 4000 BCC (50 w/v) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 15 μl of sodium cacodylate BCC pH 7.3 (1 M) 100 μl of potassium thiocyanate BCC (2 M) 550 μl of water (100 v/v) |
F12 |
| pH 6.5 | 13 μl of sodium MES BCC pH 5.2 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 36 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of calcium acetate BCC (1 M) 450 μl of water (100 v/v) |
G1 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 100 μl of calcium chloride BCC (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 500 μl of water (100 v/v) |
G2 |
| pH 6.5 | 13 μl of sodium MES BCC pH 5.2 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 36 μl of sodium MES BCC pH 7.2 (1 M) 450 μl of water (100 v/v) |
G3 |
| pH 7 | 160 μl of DL-malate-imidazole BCC pH 9 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 40 μl of DL-malate-imidazole BCC pH 2 (1 M) 500 μl of water (100 v/v) |
G4 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 125 μl of 2-propanol BCC (80 v/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 475 μl of water (100 v/v) |
G5 |
| pH 7 | 9 μl of sodium HEPES BCC pH 8.5 (1 M) 40 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 450 μl of water (100 v/v) |
G6 |
| pH 7.5 | 25 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 300 μl of sodium acetate BCC (1 M) 350 μl of water (100 v/v) |
G7 |
| pH 8 | 45 μl of tris chloride BCC pH 9 (1 M) 54 μl of tris chloride BCC pH 7 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 50 μl of sodium chloride BCC (2 M) 550 μl of water (100 v/v) |
G8 |
| pH 8 | 22 μl of tris chloride BCC pH 9 (1 M) 27 μl of tris chloride BCC pH 7 (1 M) 300 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of sodium formate BCC (4 M) 450 μl of water (100 v/v) |
G9 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 250 μl of 2-propanol BCC (80 v/v) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250 μl of water (100 v/v) |
G10 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 25 μl of tert-butanol BCC (100 v/v) 475 μl of water (100 v/v) |
G11 |
| pH 6 | 136 μl of DL-malate-imidazole BCC pH 9 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 64 μl of DL-malate-imidazole BCC pH 2 (1 M) 400 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 125 μl of 2-propanol BCC (80 v/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 375 μl of water (100 v/v) |
H1 |
| pH 7.5 | 25 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of cadmium chloride BCC (0.1 M) 25 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 500 μl of water (100 v/v) |
H2 |
| pH 8.5 | 39 μl of tris chloride BCC pH 9 (1 M) 10 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 5 μl of nickel(II) chloride BCC (1 M) 545 μl of water (100 v/v) |
H3 |
| pH 9.4 | 400 μl of polyethylene glycol 4000 BCC (50 w/v) 100 μl of magnesium chloride BCC (1 M) 100 μl of sodium bicine BCC pH 9.4 (1 M) 400 μl of water (100 v/v) |
H4 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
H5 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of water (100 v/v) |
H6 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of potassium chloride BCC (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of water (100 v/v) |
H7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 400 μl of water (100 v/v) |
H8 |
| NA | 600 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of ammonium sulfate BCC (1 M) 200 μl of water (100 v/v) |
H9 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 40 μl of ammonium acetate BCC (5 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 260 μl of water (100 v/v) |
H10 |
| pH 6 | 136 μl of DL-malate-imidazole BCC pH 9 (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 64 μl of DL-malate-imidazole BCC pH 2 (1 M) 200 μl of water (100 v/v) |
H11 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 100 μl of water (100 v/v) |
H12 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of sodium acetate BCC (1 M) 100 μl of water (100 v/v) |