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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
c3_3
BCC Core Screens / c3_3
Created 2025-07-17.
Peggy: 3350 and 4000, diverse buffers, pH range and salts.
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c3_3
Barcodes
c3_3
Screen Structure
c3_3
Well Count | 96 |
---|---|
Number of distinct ingredients | 53 |
Internal diversity (score mean dist) | 0.854 |
Screen Composition
ingredient type | c3_3 |
---|---|
Buffer | 69 |
Organic (volatile) | 4 |
Polymer | 96 |
Salt | 81 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 80.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 520.0 μl of water (100 v/v) |
A2 |
| NA | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A3 |
| NA | 200.0 μl of magnesium formate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A4 |
| NA | 200.0 μl of magnesium nitrate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A5 |
| NA | 200.0 μl of magnesium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A6 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of potassium acetate BCC (5 M) 560.0 μl of water (100 v/v) |
A7 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium chloride BCC (1 M) 400.0 μl of water (100 v/v) |
A8 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium dihydrogen phosphate BCC (1 M) 400.0 μl of water (100 v/v) |
A9 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium fluoride BCC (1 M) 400.0 μl of water (100 v/v) |
A10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium formate BCC (1 M) 400.0 μl of water (100 v/v) |
A11 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of potassium iodide BCC (5 M) 560.0 μl of water (100 v/v) |
A12 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium nitrate BCC (1 M) 400.0 μl of water (100 v/v) |
B1 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium sodium tartrate BCC (2 M) 500.0 μl of water (100 v/v) |
B2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of potassium sulfate BCC (0.5 M) 200.0 μl of water (100 v/v) |
B3 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
B4 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 400.0 μl of water (100 v/v) |
B5 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 500.0 μl of water (100 v/v) |
B6 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium dihydrogen phosphate BCC (1 M) 400.0 μl of water (100 v/v) |
B7 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium fluoride BCC (1 M) 400.0 μl of water (100 v/v) |
B8 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium formate BCC (4 M) 550.0 μl of water (100 v/v) |
B9 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium iodide BCC (1 M) 400.0 μl of water (100 v/v) |
B10 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 541.2 μl of water (100 v/v) |
B11 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium nitrate BCC (1 M) 400.0 μl of water (100 v/v) |
B12 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 166.7 μl of sodium sulfate BCC (1.2 M) 433.3 μl of water (100 v/v) |
C1 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
C2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of triammonium citrate BCC (2.5 M) 520.0 μl of water (100 v/v) |
C3 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of trilithium citrate BCC (1 M) 400.0 μl of water (100 v/v) |
C4 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of tripotassium citrate BCC (1 M) 400.0 μl of water (100 v/v) |
C5 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 125.0 μl of trisodium citrate BCC (1.6 M) 475.0 μl of water (100 v/v) |
C6 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of zinc acetate BCC (1 M) 580.0 μl of water (100 v/v) |
C7 |
| pH 6.5 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 500.0 μl of water (100 v/v) |
C8 |
| pH 7 | 100.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 127.7 μl of sodium MOPS BCC pH 6.2 (0.5 M) 72.3 μl of sodium MOPS BCC pH 8.2 (0.5 M) 300.0 μl of water (100 v/v) |
C9 |
| pH 3.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
C10 |
| pH 4.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 400.0 μl of water (100 v/v) |
C11 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
C12 |
| pH 5.5 | 40.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 360.0 μl of water (100 v/v) |
D1 |
| pH 5.5 | 200.0 μl of ammonium sulfate BCC (1 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D2 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D3 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D4 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 300.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
D6 |
| pH 6.5 | 40.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 360.0 μl of water (100 v/v) |
D7 |
| pH 6.5 | 200.0 μl of ammonium sulfate BCC (1 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D8 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D9 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 300.0 μl of water (100 v/v) |
D11 |
| pH 7.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D12 |
| pH 7.5 | 40.0 μl of ammonium acetate BCC (5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 360.0 μl of water (100 v/v) |
E1 |
| pH 7.5 | 200.0 μl of ammonium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
E4 |
| pH 7.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 100.0 μl of sodium chloride BCC (2 M) 300.0 μl of water (100 v/v) |
E5 |
| pH 8.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 400.0 μl of water (100 v/v) |
E6 |
| pH 8.5 | 40.0 μl of ammonium acetate BCC (5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 360.0 μl of water (100 v/v) |
E7 |
| pH 8.5 | 200.0 μl of ammonium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
E8 |
| pH 8.5 | 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
E9 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
E10 |
| pH 8.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
E11 |
| pH 5 | 160.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 740.0 μl of water (100 v/v) |
E12 |
| pH 6 | 160.0 μl of polyethylene glycol 4000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 740.0 μl of water (100 v/v) |
F1 |
| pH 6 | 64.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 136.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 160.0 μl of polyethylene glycol 4000 BCC (50 w/v) 640.0 μl of water (100 v/v) |
F2 |
| pH 5 | 200.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 700.0 μl of water (100 v/v) |
F3 |
| pH 5 | 200.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 200.0 μl of water (100 v/v) |
F4 |
| pH 7 | 40.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 160.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 200.0 μl of polyethylene glycol 4000 BCC (50 w/v) 600.0 μl of water (100 v/v) |
F5 |
| pH 5.5 | 240.0 μl of polyethylene glycol 4000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 660.0 μl of water (100 v/v) |
F6 |
| pH 5.5 | 100.0 μl of ammonium sulfate BCC (1 M) 240.0 μl of polyethylene glycol 4000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 560.0 μl of water (100 v/v) |
F7 |
| pH 5 | 1.7 μl of dipotassium tartrate BCC (3 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 598.3 μl of water (100 v/v) |
F8 |
| pH 5.5 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of potassium bromide BCC (1 M) 40.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 450.0 μl of water (100 v/v) |
F9 |
| pH 6 | 64.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 136.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 100.0 μl of magnesium chloride BCC (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 400.0 μl of water (100 v/v) |
F10 |
| pH 6 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 600.0 μl of water (100 v/v) |
F11 |
| pH 6 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 100.0 μl of potassium thiocyanate BCC (2 M) 34.6 μl of sodium cacodylate BCC pH 5.3 (1 M) 15.4 μl of sodium cacodylate BCC pH 7.3 (1 M) 550.0 μl of water (100 v/v) |
F12 |
| pH 6.5 | 200.0 μl of calcium acetate BCC (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 450.0 μl of water (100 v/v) |
G1 |
| pH 6.5 | 100.0 μl of calcium chloride BCC (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 500.0 μl of water (100 v/v) |
G2 |
| pH 6.5 | 200.0 μl of lithium sulfate BCC (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 450.0 μl of water (100 v/v) |
G3 |
| pH 7 | 40.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 160.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 500.0 μl of water (100 v/v) |
G4 |
| pH 7 | 125.0 μl of 2-propanol BCC (80 v/v) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 475.0 μl of water (100 v/v) |
G5 |
| pH 7 | 200.0 μl of magnesium chloride BCC (1 M) 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 40.9 μl of sodium HEPES BCC pH 6.5 (1 M) 9.1 μl of sodium HEPES BCC pH 8.5 (1 M) 450.0 μl of water (100 v/v) |
G6 |
| pH 7.5 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of sodium acetate BCC (1 M) 350.0 μl of water (100 v/v) |
G7 |
| pH 8 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium chloride BCC (2 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 550.0 μl of water (100 v/v) |
G8 |
| pH 8 | 300.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of sodium formate BCC (4 M) 27.3 μl of tris chloride BCC pH 7 (1 M) 22.7 μl of tris chloride BCC pH 9 (1 M) 450.0 μl of water (100 v/v) |
G9 |
| pH 5.5 | 250.0 μl of 2-propanol BCC (80 v/v) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250.0 μl of water (100 v/v) |
G10 |
| pH 5.5 | 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 25.0 μl of tert-butanol BCC (100 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 475.0 μl of water (100 v/v) |
G11 |
| pH 6 | 64.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 136.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 400.0 μl of water (100 v/v) |
G12 |
| pH 7.5 | 125.0 μl of 2-propanol BCC (80 v/v) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 375.0 μl of water (100 v/v) |
H1 |
| pH 7.5 | 50.0 μl of cadmium chloride BCC (0.1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
H2 |
| pH 8.5 | 5.0 μl of nickel(II) chloride BCC (1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 545.0 μl of water (100 v/v) |
H3 |
| pH 9.4 | 100.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 100.0 μl of sodium bicine BCC pH 9.4 (1 M) 400.0 μl of water (100 v/v) |
H4 |
| pH 5 | 200.0 μl of ammonium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 200.0 μl of water (100 v/v) |
H5 |
| pH 6.5 | 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 200.0 μl of water (100 v/v) |
H6 |
| pH 6.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of potassium chloride BCC (1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 200.0 μl of water (100 v/v) |
H7 |
| pH 7.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
H8 |
| NA | 200.0 μl of ammonium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of water (100 v/v) |
H9 |
| pH 5.5 | 40.0 μl of ammonium acetate BCC (5 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 260.0 μl of water (100 v/v) |
H10 |
| pH 6 | 64.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 136.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of water (100 v/v) |
H11 |
| pH 8.5 | 200.0 μl of lithium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
H12 |
| pH 8.5 | 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |