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Supplemental Information

Screen Description

c3_1

Created 2025-03-07.
Peggy: Low MW, diverse buffers, pH range and salts

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Barcodes

c3_1

  • Z0002474
  • Z0002569
  • Z0002636
  • Z0002568

Screen Structure

c3_1

Well Count 96
Number of distinct ingredients 46
Internal diversity (score mean dist) 0.932

Screen Composition

2025-06-14T14:00:02.002676 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type c3_1
Buffer 98
Organic (non-volatile) 9
Organic (volatile) 4
Polymer 110
Salt 64

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 200 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
375 μl of polyethylene glycol 200 BCC (80 v/v)
20 μl of sodium chloride BCC (5 M)
505 μl of water (100 v/v)
A2
  • polyethylene glycol 200 BCC 30 v/v -
  • polyethylene glycol 3000 BCC 5 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
375 μl of polyethylene glycol 200 BCC (80 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
100 μl of polyethylene glycol 3000 BCC (50 w/v)
425 μl of water (100 v/v)
A3
  • diammonium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 200 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
57 μl of diammonium hydrogen phosphate BCC (3.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
375 μl of polyethylene glycol 200 BCC (80 v/v)
467 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 0.2 M -
  • CAPS BCC 0.1 M pH 10
  • polyethylene glycol 200 BCC 30 v/v -
pH 10
86 μl of CAPS BCC pH 9.4 (0.5 M)
57 μl of ammonium sulfate BCC (3.5 M)
375 μl of polyethylene glycol 200 BCC (80 v/v)
114 μl of CAPS BCC pH 11 (0.5 M)
367 μl of water (100 v/v)
A5
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
pH 4.2
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
40 μl of sodium chloride BCC (5 M)
135 μl of water (100 v/v)
A6
  • polyethylene glycol 200 BCC 50 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
275 μl of water (100 v/v)
A7
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
20 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
40 μl of sodium chloride BCC (5 M)
235 μl of water (100 v/v)
A8
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
200 μl of magnesium chloride BCC (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
75 μl of water (100 v/v)
A9
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • zinc acetate BCC 0.02 M -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
20 μl of zinc acetate BCC (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
255 μl of water (100 v/v)
A10
  • polyethylene glycol 200 BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
275 μl of water (100 v/v)
A11
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 200 BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
50 μl of lithium sulfate BCC (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
225 μl of water (100 v/v)
A12
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
625 μl of polyethylene glycol 200 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
275 μl of water (100 v/v)
B1
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 200 BCC 50 v/v -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
100 μl of polyethylene glycol 1000 BCC (50 w/v)
625 μl of polyethylene glycol 200 BCC (80 v/v)
150 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 0.2 M -
  • glycerol BCC 10 v/v -
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 300 BCC 20 v/v -
pH 4.2
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
125 μl of glycerol BCC (80 v/v)
250 μl of polyethylene glycol 300 BCC (80 v/v)
57 μl of ammonium sulfate BCC (3.5 M)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
367 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1 M -
  • glycerol BCC 10 v/v -
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 300 BCC 20 v/v -
pH 8
125 μl of glycerol BCC (80 v/v)
250 μl of polyethylene glycol 300 BCC (80 v/v)
100 μl of imidazole BCC pH 8 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
239 μl of water (100 v/v)
B4
  • glycerol BCC 10 v/v -
  • polyethylene glycol 300 BCC 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
125 μl of glycerol BCC (80 v/v)
250 μl of polyethylene glycol 300 BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
525 μl of water (100 v/v)
B5
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 300 BCC 20 v/v -
  • trisodium citrate BCC 0.2 M -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
250 μl of polyethylene glycol 300 BCC (80 v/v)
125 μl of trisodium citrate BCC (1.6 M)
500 μl of water (100 v/v)
B6
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 300 BCC 40 v/v -
pH 4.2
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
300 μl of water (100 v/v)
B7
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
40 μl of sodium chloride BCC (5 M)
360 μl of water (100 v/v)
B8
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
200 μl of calcium acetate BCC (1 M)
200 μl of water (100 v/v)
B9
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 300 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
100 μl of polyethylene glycol 1000 BCC (50 w/v)
300 μl of water (100 v/v)
B10
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
40 μl of sodium chloride BCC (5 M)
360 μl of water (100 v/v)
B11
  • polyethylene glycol 300 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
  • zinc acetate BCC 0.02 M -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
20 μl of zinc acetate BCC (1 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
45 μl of tris chloride BCC pH 9 (1 M)
380 μl of water (100 v/v)
B12
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
500 μl of polyethylene glycol 300 BCC (80 v/v)
40 μl of sodium chloride BCC (5 M)
335 μl of water (100 v/v)
C1
  • ethanol BCC 10 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 350 BCC 25 v/v -
pH 8.5
100 μl of ethanol BCC (100 v/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
312 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
487 μl of water (100 v/v)
C2
  • ethanol BCC 15 v/v -
  • polyethylene glycol monomethyl ether 350 BCC 35 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 0.2 M -
pH 5.5
150 μl of ethanol BCC (100 v/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
437 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
200 μl of magnesium sulfate BCC (1 M)
112 μl of water (100 v/v)
C3
  • polyethylene glycol 400 BCC 20 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
650 μl of water (100 v/v)
C4
  • polyethylene glycol 400 BCC 20 v/v -
  • potassium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
200 μl of potassium chloride BCC (1 M)
450 μl of water (100 v/v)
C5
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of calcium acetate BCC (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
550 μl of water (100 v/v)
C6
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
125 μl of 2-propanol BCC (80 v/v)
250 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
525 μl of water (100 v/v)
C7
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 400 BCC 20 v/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
100 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
125 μl of 2-propanol BCC (80 v/v)
250 μl of polyethylene glycol 400 BCC (80 v/v)
525 μl of water (100 v/v)
C8
  • polyethylene glycol 400 BCC 25 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • manganese(II) chloride BCC 0.02 M -
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
312 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of manganese(II) chloride BCC (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
567 μl of water (100 v/v)
C9
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200 μl of calcium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
350 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
350 μl of water (100 v/v)
C10
  • cadmium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
100 μl of cadmium chloride BCC (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
425 μl of water (100 v/v)
C11
  • calcium acetate BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of calcium acetate BCC (1 M)
505 μl of water (100 v/v)
C12
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
20 μl of calcium chloride BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
505 μl of water (100 v/v)
D1
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of lithium sulfate BCC (1 M)
325 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
100 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
57 μl of ammonium sulfate BCC (3.5 M)
467 μl of water (100 v/v)
D3
  • glycerol BCC 10 v/v -
  • polyethylene glycol 3000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125 μl of glycerol BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
100 μl of polyethylene glycol 3000 BCC (50 w/v)
300 μl of water (100 v/v)
D4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200 μl of magnesium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
325 μl of water (100 v/v)
D5
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
40 μl of sodium chloride BCC (5 M)
485 μl of water (100 v/v)
D6
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
200 μl of magnesium chloride BCC (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
325 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • trisodium citrate BCC 0.2 M -
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
125 μl of trisodium citrate BCC (1.6 M)
400 μl of water (100 v/v)
D8
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 400 BCC 30 v/v -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
500 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 0.5 M -
  • CAPS BCC 0.1 M pH 10
  • glycerol BCC 10 v/v -
  • polyethylene glycol 400 BCC 30 v/v -
pH 10
86 μl of CAPS BCC pH 9.4 (0.5 M)
125 μl of glycerol BCC (80 v/v)
375 μl of polyethylene glycol 400 BCC (80 v/v)
142 μl of ammonium sulfate BCC (3.5 M)
114 μl of CAPS BCC pH 11 (0.5 M)
157 μl of water (100 v/v)
D10
  • CAPS BCC 0.1 M pH 10
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 30 v/v -
pH 10
86 μl of CAPS BCC pH 9.4 (0.5 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of polyethylene glycol 1000 BCC (50 w/v)
114 μl of CAPS BCC pH 11 (0.5 M)
325 μl of water (100 v/v)
D11
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
400 μl of water (100 v/v)
D12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200 μl of magnesium chloride BCC (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
200 μl of water (100 v/v)
E1
  • polyethylene glycol 3000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
100 μl of polyethylene glycol 3000 BCC (50 w/v)
300 μl of water (100 v/v)
E2
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
20 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
40 μl of sodium chloride BCC (5 M)
360 μl of water (100 v/v)
E3
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of calcium acetate BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
200 μl of water (100 v/v)
E4
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 400 BCC 40 v/v -
pH 8
500 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of imidazole BCC pH 8 (1 M)
400 μl of water (100 v/v)
E5
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of water (100 v/v)
E6
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
500 μl of polyethylene glycol 400 BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of lithium sulfate BCC (1 M)
200 μl of water (100 v/v)
E7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
625 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of lithium sulfate BCC (1 M)
75 μl of water (100 v/v)
E8
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
625 μl of polyethylene glycol 400 BCC (80 v/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
275 μl of water (100 v/v)
E9
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
625 μl of polyethylene glycol 400 BCC (80 v/v)
40 μl of sodium chloride BCC (5 M)
210 μl of water (100 v/v)
E10
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium acetate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
pH 5.5
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
40 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
300 μl of sodium acetate BCC (1 M)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
235 μl of water (100 v/v)
E11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium cacodylate BCC 0.05 M pH 6
pH 6
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
15 μl of sodium cacodylate BCC pH 7.3 (1 M)
200 μl of lithium sulfate BCC (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
335 μl of water (100 v/v)
E12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
36 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of magnesium chloride BCC (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
13 μl of sodium MES BCC pH 5.2 (1 M)
335 μl of water (100 v/v)
F1
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium HEPES BCC 0.05 M pH 7
  • potassium bromide BCC 0.2 M -
pH 7
9 μl of sodium HEPES BCC pH 8.5 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
200 μl of potassium bromide BCC (1 M)
335 μl of water (100 v/v)
F2
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • potassium thiocyanate BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
100 μl of potassium thiocyanate BCC (2 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
435 μl of water (100 v/v)
F3
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
pH 8
27 μl of tris chloride BCC pH 7 (1 M)
22 μl of tris chloride BCC pH 9 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
200 μl of sodium formate BCC (4 M)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
335 μl of water (100 v/v)
F4
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
10 μl of tris chloride BCC pH 7 (1 M)
200 μl of calcium acetate BCC (1 M)
39 μl of tris chloride BCC pH 9 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
94 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
335 μl of water (100 v/v)
F5
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium chloride BCC 2 M -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
100 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
235 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
400 μl of sodium chloride BCC (5 M)
264 μl of water (100 v/v)
F6
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
75 μl of potassium thiocyanate BCC (2 M)
10 μl of tris chloride BCC pH 7 (1 M)
39 μl of tris chloride BCC pH 9 (1 M)
235 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
639 μl of water (100 v/v)
F7
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • copper(II) sulfate BCC 0.01 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
10 μl of copper(II) sulfate BCC (1 M)
294 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
595 μl of water (100 v/v)
F8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
50 μl of calcium chloride BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497 μl of water (100 v/v)
F9
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of magnesium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497 μl of water (100 v/v)
F10
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium bicine BCC 0.1 M pH 9
pH 9
87 μl of sodium bicine BCC pH 9.4 (1 M)
12 μl of sodium bicine BCC pH 7.4 (1 M)
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20 μl of sodium chloride BCC (5 M)
527 μl of water (100 v/v)
F11
  • polyethylene glycol 600 BCC 15 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
250 μl of polyethylene glycol 600 BCC (60 w/v)
68 μl of DL-malate-imidazole BCC pH 2 (1 M)
132 μl of DL-malate-imidazole BCC pH 9 (1 M)
550 μl of water (100 v/v)
F12
  • polyethylene glycol 600 BCC 18 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
300 μl of polyethylene glycol 600 BCC (60 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
600 μl of water (100 v/v)
G1
  • polyethylene glycol 600 BCC 20 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
75 μl of potassium thiocyanate BCC (2 M)
10 μl of tris chloride BCC pH 7 (1 M)
333 μl of polyethylene glycol 600 BCC (60 w/v)
39 μl of tris chloride BCC pH 9 (1 M)
541 μl of water (100 v/v)
G2
  • polyethylene glycol 600 BCC 24 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
400 μl of polyethylene glycol 600 BCC (60 w/v)
68 μl of DL-malate-imidazole BCC pH 2 (1 M)
132 μl of DL-malate-imidazole BCC pH 9 (1 M)
400 μl of water (100 v/v)
G3
  • polyethylene glycol 600 BCC 27 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
450 μl of polyethylene glycol 600 BCC (60 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
450 μl of water (100 v/v)
G4
  • glycerol BCC 10 v/v -
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
500 μl of polyethylene glycol 600 BCC (60 w/v)
38 μl of sodium MES BCC pH 7.2 (1 M)
125 μl of glycerol BCC (80 v/v)
100 μl of polyethylene glycol 1000 BCC (50 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
175 μl of water (100 v/v)
G5
  • glycerol BCC 10 v/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 1 M -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
500 μl of polyethylene glycol 600 BCC (60 w/v)
125 μl of glycerol BCC (80 v/v)
200 μl of sodium chloride BCC (5 M)
75 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 0.5 M -
  • glycerol BCC 10 v/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
500 μl of polyethylene glycol 600 BCC (60 w/v)
125 μl of glycerol BCC (80 v/v)
142 μl of ammonium sulfate BCC (3.5 M)
132 μl of water (100 v/v)
G7
  • glycerol BCC 10 v/v -
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 600 BCC (60 w/v)
125 μl of glycerol BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of lithium sulfate BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
225 μl of water (100 v/v)
G8
  • polyethylene glycol 600 BCC 33 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
550 μl of polyethylene glycol 600 BCC (60 w/v)
68 μl of DL-malate-imidazole BCC pH 2 (1 M)
132 μl of DL-malate-imidazole BCC pH 9 (1 M)
250 μl of water (100 v/v)
G9
  • polyethylene glycol 600 BCC 36 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600 μl of polyethylene glycol 600 BCC (60 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
300 μl of water (100 v/v)
G10
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 600 BCC 40 w/v -
pH 4.2
666 μl of polyethylene glycol 600 BCC (60 w/v)
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
133 μl of water (100 v/v)
G11
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 600 BCC 40 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
666 μl of polyethylene glycol 600 BCC (60 w/v)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
200 μl of magnesium chloride BCC (1 M)
33 μl of water (100 v/v)
G12
  • polyethylene glycol 600 BCC 40 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
666 μl of polyethylene glycol 600 BCC (60 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
233 μl of water (100 v/v)
H1
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 600 BCC 40 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
666 μl of polyethylene glycol 600 BCC (60 w/v)
200 μl of calcium acetate BCC (1 M)
33 μl of water (100 v/v)
H2
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 600 BCC 40 w/v -
  • zinc acetate BCC 0.02 M -
pH 8
666 μl of polyethylene glycol 600 BCC (60 w/v)
20 μl of zinc acetate BCC (1 M)
100 μl of imidazole BCC pH 8 (1 M)
213 μl of water (100 v/v)
H3
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 600 BCC 40 w/v -
pH 9.5
666 μl of polyethylene glycol 600 BCC (60 w/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
208 μl of water (100 v/v)
H4
  • polyethylene glycol 600 BCC 42 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
700 μl of polyethylene glycol 600 BCC (60 w/v)
68 μl of DL-malate-imidazole BCC pH 2 (1 M)
132 μl of DL-malate-imidazole BCC pH 9 (1 M)
100 μl of water (100 v/v)
H5
  • polyethylene glycol 600 BCC 45 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
750 μl of polyethylene glycol 600 BCC (60 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
150 μl of water (100 v/v)
H6
  • polyethylene glycol monomethyl ether 750 BCC 12 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
150 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
750 μl of water (100 v/v)
H7
  • sodium cacodylate BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 750 BCC 20 w/v -
pH 6
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
250 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
650 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 750 BCC 20 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
250 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
650 μl of water (100 v/v)
H9
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 750 BCC 30 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
525 μl of water (100 v/v)
H10
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 30 w/v -
pH 8.2
90 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9 μl of sodium HEPES BCC pH 6.5 (1 M)
525 μl of water (100 v/v)
H11
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 40 w/v -
pH 8.2
90 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9 μl of sodium HEPES BCC pH 6.5 (1 M)
400 μl of water (100 v/v)
H12
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 50 w/v -
pH 8.2
90 μl of sodium HEPES BCC pH 8.5 (1 M)
625 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9 μl of sodium HEPES BCC pH 6.5 (1 M)
275 μl of water (100 v/v)