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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
c3_1
Barcodes
c3_1
Screen Structure
c3_1
Well Count | 96 |
---|---|
Number of distinct ingredients | 46 |
Internal diversity (score mean dist) | 0.932 |
Screen Composition
ingredient type | c3_1 |
---|---|
Buffer | 98 |
Organic (non-volatile) | 9 |
Organic (volatile) | 4 |
Polymer | 110 |
Salt | 64 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5 | 375.0 μl of polyethylene glycol 200 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 20.0 μl of sodium chloride BCC (5 M) 505.0 μl of water (100 v/v) |
A2 |
| pH 6.5 | 375.0 μl of polyethylene glycol 200 BCC (80 v/v) 100.0 μl of polyethylene glycol 3000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 425.0 μl of water (100 v/v) |
A3 |
| pH 8.5 | 57.1 μl of diammonium hydrogen phosphate BCC (3.5 M) 375.0 μl of polyethylene glycol 200 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 467.9 μl of water (100 v/v) |
A4 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 57.1 μl of ammonium sulfate BCC (3.5 M) 375.0 μl of polyethylene glycol 200 BCC (80 v/v) 367.9 μl of water (100 v/v) |
A5 |
| pH 4.2 | 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 40.0 μl of sodium chloride BCC (5 M) 135.0 μl of water (100 v/v) |
A6 |
| pH 5.5 | 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 275.0 μl of water (100 v/v) |
A7 |
| pH 6.2 | 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 40.0 μl of sodium chloride BCC (5 M) 20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M) 80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M) 235.0 μl of water (100 v/v) |
A8 |
| pH 6.5 | 200.0 μl of magnesium chloride BCC (1 M) 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 75.0 μl of water (100 v/v) |
A9 |
| pH 6.5 | 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 20.0 μl of zinc acetate BCC (1 M) 255.0 μl of water (100 v/v) |
A10 |
| pH 7 | 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 275.0 μl of water (100 v/v) |
A11 |
| pH 7 | 50.0 μl of lithium sulfate BCC (1 M) 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 225.0 μl of water (100 v/v) |
A12 |
| pH 7.5 | 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 275.0 μl of water (100 v/v) |
B1 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 100.0 μl of polyethylene glycol 1000 BCC (50 w/v) 625.0 μl of polyethylene glycol 200 BCC (80 v/v) 150.0 μl of water (100 v/v) |
B2 |
| pH 4.2 | 57.1 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 250.0 μl of polyethylene glycol 300 BCC (80 v/v) 367.9 μl of water (100 v/v) |
B3 |
| pH 8 | 285.7 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 100.0 μl of imidazole BCC pH 8 (1 M) 250.0 μl of polyethylene glycol 300 BCC (80 v/v) 239.3 μl of water (100 v/v) |
B4 |
| pH 8.5 | 125.0 μl of glycerol BCC (80 v/v) 250.0 μl of polyethylene glycol 300 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 525.0 μl of water (100 v/v) |
B5 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 250.0 μl of polyethylene glycol 300 BCC (80 v/v) 125.0 μl of trisodium citrate BCC (1.6 M) 500.0 μl of water (100 v/v) |
B6 |
| pH 4.2 | 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 300.0 μl of water (100 v/v) |
B7 |
| pH 5 | 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
B8 |
| pH 6.5 | 200.0 μl of calcium acetate BCC (1 M) 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 200.0 μl of water (100 v/v) |
B9 |
| pH 7 | 100.0 μl of polyethylene glycol 1000 BCC (50 w/v) 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 300.0 μl of water (100 v/v) |
B10 |
| pH 7.5 | 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
B11 |
| pH 8 | 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 20.0 μl of zinc acetate BCC (1 M) 380.0 μl of water (100 v/v) |
B12 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 500.0 μl of polyethylene glycol 300 BCC (80 v/v) 40.0 μl of sodium chloride BCC (5 M) 335.0 μl of water (100 v/v) |
C1 |
| pH 8.5 | 100.0 μl of ethanol BCC (100 v/v) 312.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 487.5 μl of water (100 v/v) |
C2 |
| pH 5.5 | 150.0 μl of ethanol BCC (100 v/v) 200.0 μl of magnesium sulfate BCC (1 M) 437.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 112.5 μl of water (100 v/v) |
C3 |
| pH 5.5 | 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 650.0 μl of water (100 v/v) |
C4 |
| pH 5.5 | 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 200.0 μl of potassium chloride BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 450.0 μl of water (100 v/v) |
C5 |
| pH 7 | 100.0 μl of calcium acetate BCC (1 M) 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 550.0 μl of water (100 v/v) |
C6 |
| pH 7.5 | 125.0 μl of 2-propanol BCC (80 v/v) 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 525.0 μl of water (100 v/v) |
C7 |
| pH 7.5 | 125.0 μl of 2-propanol BCC (80 v/v) 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M) 525.0 μl of water (100 v/v) |
C8 |
| pH 6.5 | 20.0 μl of manganese(II) chloride BCC (1 M) 312.5 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 567.5 μl of water (100 v/v) |
C9 |
| pH 7.5 | 200.0 μl of calcium chloride BCC (1 M) 350.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
C10 |
| pH 5 | 100.0 μl of cadmium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 425.0 μl of water (100 v/v) |
C11 |
| pH 5 | 20.0 μl of calcium acetate BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 505.0 μl of water (100 v/v) |
C12 |
| pH 6.5 | 20.0 μl of calcium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 505.0 μl of water (100 v/v) |
D1 |
| pH 6.5 | 200.0 μl of lithium sulfate BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 325.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M) 467.9 μl of water (100 v/v) |
D3 |
| pH 7.5 | 125.0 μl of glycerol BCC (80 v/v) 100.0 μl of polyethylene glycol 3000 BCC (50 w/v) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 200.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 325.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 40.0 μl of sodium chloride BCC (5 M) 485.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 325.0 μl of water (100 v/v) |
D7 |
| pH 8.5 | 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 125.0 μl of trisodium citrate BCC (1.6 M) 400.0 μl of water (100 v/v) |
D8 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 500.0 μl of water (100 v/v) |
D9 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 142.9 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 157.1 μl of water (100 v/v) |
D10 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 100.0 μl of polyethylene glycol 1000 BCC (50 w/v) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 325.0 μl of water (100 v/v) |
D11 |
| pH 5 | 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 400.0 μl of water (100 v/v) |
D12 |
| pH 5.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 200.0 μl of water (100 v/v) |
E1 |
| pH 6 | 100.0 μl of polyethylene glycol 3000 BCC (50 w/v) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
E2 |
| pH 6.2 | 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 40.0 μl of sodium chloride BCC (5 M) 20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M) 80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M) 360.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 200.0 μl of calcium acetate BCC (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
E4 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 400.0 μl of water (100 v/v) |
E5 |
| pH 8.5 | 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 400.0 μl of water (100 v/v) |
E6 |
| pH 8.5 | 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
E7 |
| pH 5 | 200.0 μl of lithium sulfate BCC (1 M) 625.0 μl of polyethylene glycol 400 BCC (80 v/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 75.0 μl of water (100 v/v) |
E8 |
| pH 6 | 625.0 μl of polyethylene glycol 400 BCC (80 v/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 275.0 μl of water (100 v/v) |
E9 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 625.0 μl of polyethylene glycol 400 BCC (80 v/v) 40.0 μl of sodium chloride BCC (5 M) 210.0 μl of water (100 v/v) |
E10 |
| pH 5.5 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 300.0 μl of sodium acetate BCC (1 M) 40.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 235.9 μl of water (100 v/v) |
E11 |
| pH 6 | 200.0 μl of lithium sulfate BCC (1 M) 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 34.6 μl of sodium cacodylate BCC pH 5.3 (1 M) 15.4 μl of sodium cacodylate BCC pH 7.3 (1 M) 335.9 μl of water (100 v/v) |
E12 |
| pH 6.5 | 200.0 μl of magnesium chloride BCC (1 M) 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 335.9 μl of water (100 v/v) |
F1 |
| pH 7 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 200.0 μl of potassium bromide BCC (1 M) 40.9 μl of sodium HEPES BCC pH 6.5 (1 M) 9.1 μl of sodium HEPES BCC pH 8.5 (1 M) 335.9 μl of water (100 v/v) |
F2 |
| pH 7.5 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 100.0 μl of potassium thiocyanate BCC (2 M) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 435.9 μl of water (100 v/v) |
F3 |
| pH 8 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 200.0 μl of sodium formate BCC (4 M) 27.3 μl of tris chloride BCC pH 7 (1 M) 22.7 μl of tris chloride BCC pH 9 (1 M) 335.9 μl of water (100 v/v) |
F4 |
| pH 8.5 | 200.0 μl of calcium acetate BCC (1 M) 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 335.9 μl of water (100 v/v) |
F5 |
| pH 7.5 | 235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 400.0 μl of sodium chloride BCC (5 M) 100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M) 264.7 μl of water (100 v/v) |
F6 |
| pH 8.5 | 235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 75.0 μl of potassium thiocyanate BCC (2 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 639.7 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 10.0 μl of copper(II) sulfate BCC (1 M) 294.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 595.9 μl of water (100 v/v) |
F8 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 50.0 μl of calcium chloride BCC (1 M) 352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 497.1 μl of water (100 v/v) |
F9 |
| pH 7.5 | 50.0 μl of magnesium chloride BCC (1 M) 352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 497.1 μl of water (100 v/v) |
F10 |
| pH 9 | 352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 12.4 μl of sodium bicine BCC pH 7.4 (1 M) 87.6 μl of sodium bicine BCC pH 9.4 (1 M) 20.0 μl of sodium chloride BCC (5 M) 527.1 μl of water (100 v/v) |
F11 |
| pH 5.5 | 68.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 132.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 250.0 μl of polyethylene glycol 600 BCC (60 w/v) 550.0 μl of water (100 v/v) |
F12 |
| pH 7.5 | 300.0 μl of polyethylene glycol 600 BCC (60 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
G1 |
| pH 8.5 | 333.3 μl of polyethylene glycol 600 BCC (60 w/v) 75.0 μl of potassium thiocyanate BCC (2 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 541.7 μl of water (100 v/v) |
G2 |
| pH 5.5 | 68.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 132.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 400.0 μl of polyethylene glycol 600 BCC (60 w/v) 400.0 μl of water (100 v/v) |
G3 |
| pH 7.5 | 450.0 μl of polyethylene glycol 600 BCC (60 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 450.0 μl of water (100 v/v) |
G4 |
| pH 6 | 125.0 μl of glycerol BCC (80 v/v) 100.0 μl of polyethylene glycol 1000 BCC (50 w/v) 500.0 μl of polyethylene glycol 600 BCC (60 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 175.0 μl of water (100 v/v) |
G5 |
| pH 6.5 | 125.0 μl of glycerol BCC (80 v/v) 500.0 μl of polyethylene glycol 600 BCC (60 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 200.0 μl of sodium chloride BCC (5 M) 75.0 μl of water (100 v/v) |
G6 |
| pH 7 | 142.9 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 500.0 μl of polyethylene glycol 600 BCC (60 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 132.1 μl of water (100 v/v) |
G7 |
| pH 7.5 | 125.0 μl of glycerol BCC (80 v/v) 50.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 600 BCC (60 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 225.0 μl of water (100 v/v) |
G8 |
| pH 5.5 | 68.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 132.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 550.0 μl of polyethylene glycol 600 BCC (60 w/v) 250.0 μl of water (100 v/v) |
G9 |
| pH 7.5 | 600.0 μl of polyethylene glycol 600 BCC (60 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G10 |
| pH 4.2 | 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 133.3 μl of water (100 v/v) |
G11 |
| pH 5 | 200.0 μl of magnesium chloride BCC (1 M) 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 33.3 μl of water (100 v/v) |
G12 |
| pH 5.5 | 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 233.3 μl of water (100 v/v) |
H1 |
| pH 6.5 | 200.0 μl of calcium acetate BCC (1 M) 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 33.3 μl of water (100 v/v) |
H2 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 20.0 μl of zinc acetate BCC (1 M) 213.3 μl of water (100 v/v) |
H3 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 666.7 μl of polyethylene glycol 600 BCC (60 w/v) 208.3 μl of water (100 v/v) |
H4 |
| pH 5.5 | 68.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 132.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 700.0 μl of polyethylene glycol 600 BCC (60 w/v) 100.0 μl of water (100 v/v) |
H5 |
| pH 7.5 | 750.0 μl of polyethylene glycol 600 BCC (60 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 150.0 μl of water (100 v/v) |
H6 |
| pH 5 | 150.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 750.0 μl of water (100 v/v) |
H7 |
| pH 6 | 250.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 650.0 μl of water (100 v/v) |
H8 |
| pH 8 | 250.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 650.0 μl of water (100 v/v) |
H9 |
| pH 7.5 | 375.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 525.0 μl of water (100 v/v) |
H10 |
| pH 8.2 | 375.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 9.2 μl of sodium HEPES BCC pH 6.5 (1 M) 90.8 μl of sodium HEPES BCC pH 8.5 (1 M) 525.0 μl of water (100 v/v) |
H11 |
| pH 8.2 | 500.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 9.2 μl of sodium HEPES BCC pH 6.5 (1 M) 90.8 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
H12 |
| pH 8.2 | 625.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 9.2 μl of sodium HEPES BCC pH 6.5 (1 M) 90.8 μl of sodium HEPES BCC pH 8.5 (1 M) 275.0 μl of water (100 v/v) |