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Supplemental Information

Screen Description

c3_1

Created 2025-03-07.
Peggy: Low MW, diverse buffers, pH range and salts

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c3_1

  • Z0002568 Lot 4 (1500.0 µl)
  • Z0002569 Lot 2 (1500.0 µl)
  • Z0002474 Lot 1 (0.0 µl)
  • Z0002636 Lot 3 (0.0 µl)

Screen Structure

c3_1

Well Count 96
Number of distinct ingredients 46
Internal diversity (score mean dist) 0.932

Screen Composition

2025-08-15T11:09:52.455398 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type c3_1
Buffer 98
Organic (non-volatile) 9
Organic (volatile) 4
Polymer 110
Salt 64

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 200 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
375.0 μl of polyethylene glycol 200 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20.0 μl of sodium chloride BCC (5 M)
505.0 μl of water (100 v/v)
A2
  • polyethylene glycol 200 BCC 30 v/v -
  • polyethylene glycol 3000 BCC 5 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
375.0 μl of polyethylene glycol 200 BCC (80 v/v)
100.0 μl of polyethylene glycol 3000 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
425.0 μl of water (100 v/v)
A3
  • diammonium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 200 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
57.1 μl of diammonium hydrogen phosphate BCC (3.5 M)
375.0 μl of polyethylene glycol 200 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
467.9 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 0.2 M -
  • CAPS BCC 0.1 M pH 10
  • polyethylene glycol 200 BCC 30 v/v -
pH 10
86.0 μl of CAPS BCC pH 9.4 (0.5 M)
114.0 μl of CAPS BCC pH 11 (0.5 M)
57.1 μl of ammonium sulfate BCC (3.5 M)
375.0 μl of polyethylene glycol 200 BCC (80 v/v)
367.9 μl of water (100 v/v)
A5
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
pH 4.2
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
40.0 μl of sodium chloride BCC (5 M)
135.0 μl of water (100 v/v)
A6
  • polyethylene glycol 200 BCC 50 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
275.0 μl of water (100 v/v)
A7
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
40.0 μl of sodium chloride BCC (5 M)
20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
235.0 μl of water (100 v/v)
A8
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
75.0 μl of water (100 v/v)
A9
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • zinc acetate BCC 0.02 M -
pH 6.5
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
20.0 μl of zinc acetate BCC (1 M)
255.0 μl of water (100 v/v)
A10
  • polyethylene glycol 200 BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
275.0 μl of water (100 v/v)
A11
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 200 BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
50.0 μl of lithium sulfate BCC (1 M)
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
225.0 μl of water (100 v/v)
A12
  • polyethylene glycol 200 BCC 50 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
275.0 μl of water (100 v/v)
B1
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 200 BCC 50 v/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
100.0 μl of polyethylene glycol 1000 BCC (50 w/v)
625.0 μl of polyethylene glycol 200 BCC (80 v/v)
150.0 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 0.2 M -
  • glycerol BCC 10 v/v -
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 300 BCC 20 v/v -
pH 4.2
57.1 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
250.0 μl of polyethylene glycol 300 BCC (80 v/v)
367.9 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1 M -
  • glycerol BCC 10 v/v -
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 300 BCC 20 v/v -
pH 8
285.7 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
100.0 μl of imidazole BCC pH 8 (1 M)
250.0 μl of polyethylene glycol 300 BCC (80 v/v)
239.3 μl of water (100 v/v)
B4
  • glycerol BCC 10 v/v -
  • polyethylene glycol 300 BCC 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of glycerol BCC (80 v/v)
250.0 μl of polyethylene glycol 300 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
525.0 μl of water (100 v/v)
B5
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 300 BCC 20 v/v -
  • trisodium citrate BCC 0.2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
250.0 μl of polyethylene glycol 300 BCC (80 v/v)
125.0 μl of trisodium citrate BCC (1.6 M)
500.0 μl of water (100 v/v)
B6
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 300 BCC 40 v/v -
pH 4.2
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
300.0 μl of water (100 v/v)
B7
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
B8
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of calcium acetate BCC (1 M)
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
200.0 μl of water (100 v/v)
B9
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 300 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100.0 μl of polyethylene glycol 1000 BCC (50 w/v)
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
300.0 μl of water (100 v/v)
B10
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
B11
  • polyethylene glycol 300 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
  • zinc acetate BCC 0.02 M -
pH 8
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
20.0 μl of zinc acetate BCC (1 M)
380.0 μl of water (100 v/v)
B12
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 300 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
500.0 μl of polyethylene glycol 300 BCC (80 v/v)
40.0 μl of sodium chloride BCC (5 M)
335.0 μl of water (100 v/v)
C1
  • ethanol BCC 10 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 350 BCC 25 v/v -
pH 8.5
100.0 μl of ethanol BCC (100 v/v)
312.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
487.5 μl of water (100 v/v)
C2
  • ethanol BCC 15 v/v -
  • polyethylene glycol monomethyl ether 350 BCC 35 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 0.2 M -
pH 5.5
150.0 μl of ethanol BCC (100 v/v)
200.0 μl of magnesium sulfate BCC (1 M)
437.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
112.5 μl of water (100 v/v)
C3
  • polyethylene glycol 400 BCC 20 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
650.0 μl of water (100 v/v)
C4
  • polyethylene glycol 400 BCC 20 v/v -
  • potassium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
200.0 μl of potassium chloride BCC (1 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
450.0 μl of water (100 v/v)
C5
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
100.0 μl of calcium acetate BCC (1 M)
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
C6
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-propanol BCC (80 v/v)
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
C7
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 400 BCC 20 v/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-propanol BCC (80 v/v)
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
525.0 μl of water (100 v/v)
C8
  • polyethylene glycol 400 BCC 25 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • manganese(II) chloride BCC 0.02 M -
pH 6.5
20.0 μl of manganese(II) chloride BCC (1 M)
312.5 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
567.5 μl of water (100 v/v)
C9
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of calcium chloride BCC (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
C10
  • cadmium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
100.0 μl of cadmium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
425.0 μl of water (100 v/v)
C11
  • calcium acetate BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
20.0 μl of calcium acetate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
505.0 μl of water (100 v/v)
C12
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
505.0 μl of water (100 v/v)
D1
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
325.0 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
57.1 μl of ammonium sulfate BCC (3.5 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
467.9 μl of water (100 v/v)
D3
  • glycerol BCC 10 v/v -
  • polyethylene glycol 3000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of glycerol BCC (80 v/v)
100.0 μl of polyethylene glycol 3000 BCC (50 w/v)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
325.0 μl of water (100 v/v)
D5
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
40.0 μl of sodium chloride BCC (5 M)
485.0 μl of water (100 v/v)
D6
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
325.0 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • trisodium citrate BCC 0.2 M -
pH 8.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
125.0 μl of trisodium citrate BCC (1.6 M)
400.0 μl of water (100 v/v)
D8
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 400 BCC 30 v/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
500.0 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 0.5 M -
  • CAPS BCC 0.1 M pH 10
  • glycerol BCC 10 v/v -
  • polyethylene glycol 400 BCC 30 v/v -
pH 10
86.0 μl of CAPS BCC pH 9.4 (0.5 M)
114.0 μl of CAPS BCC pH 11 (0.5 M)
142.9 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
157.1 μl of water (100 v/v)
D10
  • CAPS BCC 0.1 M pH 10
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 30 v/v -
pH 10
86.0 μl of CAPS BCC pH 9.4 (0.5 M)
114.0 μl of CAPS BCC pH 11 (0.5 M)
100.0 μl of polyethylene glycol 1000 BCC (50 w/v)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
325.0 μl of water (100 v/v)
D11
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
400.0 μl of water (100 v/v)
D12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
200.0 μl of water (100 v/v)
E1
  • polyethylene glycol 3000 BCC 5 w/v -
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
100.0 μl of polyethylene glycol 3000 BCC (50 w/v)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
E2
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.0 μl of sodium chloride BCC (5 M)
20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
360.0 μl of water (100 v/v)
E3
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of calcium acetate BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
E4
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 400 BCC 40 v/v -
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of water (100 v/v)
E5
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
E6
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
200.0 μl of water (100 v/v)
E7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of lithium sulfate BCC (1 M)
625.0 μl of polyethylene glycol 400 BCC (80 v/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
75.0 μl of water (100 v/v)
E8
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
625.0 μl of polyethylene glycol 400 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
275.0 μl of water (100 v/v)
E9
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 400 BCC 50 v/v -
  • sodium chloride BCC 0.2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
625.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.0 μl of sodium chloride BCC (5 M)
210.0 μl of water (100 v/v)
E10
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium acetate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
pH 5.5
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
300.0 μl of sodium acetate BCC (1 M)
40.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
235.9 μl of water (100 v/v)
E11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium cacodylate BCC 0.05 M pH 6
pH 6
200.0 μl of lithium sulfate BCC (1 M)
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
34.6 μl of sodium cacodylate BCC pH 5.3 (1 M)
15.4 μl of sodium cacodylate BCC pH 7.3 (1 M)
335.9 μl of water (100 v/v)
E12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
335.9 μl of water (100 v/v)
F1
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium HEPES BCC 0.05 M pH 7
  • potassium bromide BCC 0.2 M -
pH 7
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
200.0 μl of potassium bromide BCC (1 M)
40.9 μl of sodium HEPES BCC pH 6.5 (1 M)
9.1 μl of sodium HEPES BCC pH 8.5 (1 M)
335.9 μl of water (100 v/v)
F2
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • potassium thiocyanate BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of potassium thiocyanate BCC (2 M)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
435.9 μl of water (100 v/v)
F3
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
pH 8
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
200.0 μl of sodium formate BCC (4 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
335.9 μl of water (100 v/v)
F4
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 20000 BCC 8 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 8 v/v -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
200.0 μl of calcium acetate BCC (1 M)
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
94.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
10.6 μl of tris chloride BCC pH 7 (1 M)
39.4 μl of tris chloride BCC pH 9 (1 M)
335.9 μl of water (100 v/v)
F5
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium chloride BCC 2 M -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
400.0 μl of sodium chloride BCC (5 M)
100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
264.7 μl of water (100 v/v)
F6
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
75.0 μl of potassium thiocyanate BCC (2 M)
10.6 μl of tris chloride BCC pH 7 (1 M)
39.4 μl of tris chloride BCC pH 9 (1 M)
639.7 μl of water (100 v/v)
F7
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • copper(II) sulfate BCC 0.01 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
10.0 μl of copper(II) sulfate BCC (1 M)
294.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
595.9 μl of water (100 v/v)
F8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
50.0 μl of calcium chloride BCC (1 M)
352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497.1 μl of water (100 v/v)
F9
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of magnesium chloride BCC (1 M)
352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
497.1 μl of water (100 v/v)
F10
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium bicine BCC 0.1 M pH 9
pH 9
352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
12.4 μl of sodium bicine BCC pH 7.4 (1 M)
87.6 μl of sodium bicine BCC pH 9.4 (1 M)
20.0 μl of sodium chloride BCC (5 M)
527.1 μl of water (100 v/v)
F11
  • polyethylene glycol 600 BCC 15 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
68.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
132.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
250.0 μl of polyethylene glycol 600 BCC (60 w/v)
550.0 μl of water (100 v/v)
F12
  • polyethylene glycol 600 BCC 18 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
300.0 μl of polyethylene glycol 600 BCC (60 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
G1
  • polyethylene glycol 600 BCC 20 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
333.3 μl of polyethylene glycol 600 BCC (60 w/v)
75.0 μl of potassium thiocyanate BCC (2 M)
10.6 μl of tris chloride BCC pH 7 (1 M)
39.4 μl of tris chloride BCC pH 9 (1 M)
541.7 μl of water (100 v/v)
G2
  • polyethylene glycol 600 BCC 24 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
68.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
132.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
400.0 μl of polyethylene glycol 600 BCC (60 w/v)
400.0 μl of water (100 v/v)
G3
  • polyethylene glycol 600 BCC 27 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
450.0 μl of polyethylene glycol 600 BCC (60 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
450.0 μl of water (100 v/v)
G4
  • glycerol BCC 10 v/v -
  • polyethylene glycol 1000 BCC 5 w/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
125.0 μl of glycerol BCC (80 v/v)
100.0 μl of polyethylene glycol 1000 BCC (50 w/v)
500.0 μl of polyethylene glycol 600 BCC (60 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
175.0 μl of water (100 v/v)
G5
  • glycerol BCC 10 v/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 1 M -
pH 6.5
125.0 μl of glycerol BCC (80 v/v)
500.0 μl of polyethylene glycol 600 BCC (60 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
200.0 μl of sodium chloride BCC (5 M)
75.0 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 0.5 M -
  • glycerol BCC 10 v/v -
  • polyethylene glycol 600 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
142.9 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
500.0 μl of polyethylene glycol 600 BCC (60 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
132.1 μl of water (100 v/v)
G7
  • glycerol BCC 10 v/v -
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 600 BCC 30 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of glycerol BCC (80 v/v)
50.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 600 BCC (60 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
225.0 μl of water (100 v/v)
G8
  • polyethylene glycol 600 BCC 33 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
68.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
132.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
550.0 μl of polyethylene glycol 600 BCC (60 w/v)
250.0 μl of water (100 v/v)
G9
  • polyethylene glycol 600 BCC 36 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of polyethylene glycol 600 BCC (60 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G10
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 600 BCC 40 w/v -
pH 4.2
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
133.3 μl of water (100 v/v)
G11
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 600 BCC 40 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of magnesium chloride BCC (1 M)
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
33.3 μl of water (100 v/v)
G12
  • polyethylene glycol 600 BCC 40 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
233.3 μl of water (100 v/v)
H1
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 600 BCC 40 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of calcium acetate BCC (1 M)
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
33.3 μl of water (100 v/v)
H2
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 600 BCC 40 w/v -
  • zinc acetate BCC 0.02 M -
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
20.0 μl of zinc acetate BCC (1 M)
213.3 μl of water (100 v/v)
H3
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 600 BCC 40 w/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
666.7 μl of polyethylene glycol 600 BCC (60 w/v)
208.3 μl of water (100 v/v)
H4
  • polyethylene glycol 600 BCC 42 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 5.5
pH 5.5
68.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
132.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
700.0 μl of polyethylene glycol 600 BCC (60 w/v)
100.0 μl of water (100 v/v)
H5
  • polyethylene glycol 600 BCC 45 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
750.0 μl of polyethylene glycol 600 BCC (60 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
H6
  • polyethylene glycol monomethyl ether 750 BCC 12 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
150.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
750.0 μl of water (100 v/v)
H7
  • sodium cacodylate BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 750 BCC 20 w/v -
pH 6
250.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
69.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
650.0 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 750 BCC 20 w/v -
pH 8
250.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
650.0 μl of water (100 v/v)
H9
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 750 BCC 30 w/v -
pH 7.5
375.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
H10
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 30 w/v -
pH 8.2
375.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9.2 μl of sodium HEPES BCC pH 6.5 (1 M)
90.8 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
H11
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 40 w/v -
pH 8.2
500.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9.2 μl of sodium HEPES BCC pH 6.5 (1 M)
90.8 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H12
  • sodium HEPES BCC 0.1 M pH 8.2
  • polyethylene glycol monomethyl ether 750 BCC 50 w/v -
pH 8.2
625.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
9.2 μl of sodium HEPES BCC pH 6.5 (1 M)
90.8 μl of sodium HEPES BCC pH 8.5 (1 M)
275.0 μl of water (100 v/v)