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Supplemental Information

Screen Description

c3_2

Created 2025-03-07.
Peggy: Low-Mid MW, diverse buffers, pH range and salts.

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Barcodes

c3_2

  • Z0002638
  • Z0002786
  • Z0002787
  • Z0002637

Screen Structure

c3_2

Well Count 96
Number of distinct ingredients 63
Internal diversity (score mean dist) 0.934

Screen Composition

2025-06-12T22:59:02.843132 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type c3_2
Buffer 73
Organic (non-volatile) 3
Organic (volatile) 1
Polymer 104
Salt 76

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 1000 BCC 10 w/v -
  • polyethylene glycol 8000 BCC 10 w/v -
NA
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
600 μl of water (100 v/v)
A2
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium acetate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 5.5
40 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
300 μl of sodium acetate BCC (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
250 μl of water (100 v/v)
A3
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium cacodylate BCC 0.05 M pH 6
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6
15 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
200 μl of lithium sulfate BCC (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
350 μl of water (100 v/v)
A4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium MES BCC 0.05 M pH 6.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6.5
13 μl of sodium MES BCC pH 5.2 (1 M)
36 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of magnesium chloride BCC (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
350 μl of water (100 v/v)
A5
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium HEPES BCC 0.05 M pH 7
  • potassium bromide BCC 0.2 M -
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7
200 μl of potassium bromide BCC (1 M)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
9 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
350 μl of water (100 v/v)
A6
  • polyethylene glycol 1000 BCC 10 w/v -
  • potassium thiocyanate BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7.5
100 μl of potassium thiocyanate BCC (2 M)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
450 μl of water (100 v/v)
A7
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8
27 μl of tris chloride BCC pH 7 (1 M)
22 μl of tris chloride BCC pH 9 (1 M)
200 μl of sodium formate BCC (4 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
350 μl of water (100 v/v)
A8
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • tris chloride BCC 0.05 M pH 8.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8.5
200 μl of calcium acetate BCC (1 M)
10 μl of tris chloride BCC pH 7 (1 M)
39 μl of tris chloride BCC pH 9 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of polyethylene glycol 1000 BCC (50 w/v)
350 μl of water (100 v/v)
A9
  • lithium sulfate BCC 0.2 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 1000 BCC 20 w/v -
pH 4.2
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
200 μl of lithium sulfate BCC (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
200 μl of water (100 v/v)
A10
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
200 μl of sodium chloride BCC (1 M)
20 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
300 μl of water (100 v/v)
A11
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
500 μl of water (100 v/v)
A12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200 μl of magnesium chloride BCC (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
300 μl of water (100 v/v)
B1
  • polyethylene glycol 1000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
500 μl of water (100 v/v)
B2
  • calcium acetate BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 1000 BCC 20 w/v -
pH 8
200 μl of calcium acetate BCC (1 M)
100 μl of imidazole BCC pH 8 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
300 μl of water (100 v/v)
B3
  • polyethylene glycol 1000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 1000 BCC (50 w/v)
500 μl of water (100 v/v)
B4
  • polyethylene glycol 1000 BCC 25 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20 μl of zinc acetate BCC (1 M)
500 μl of polyethylene glycol 1000 BCC (50 w/v)
380 μl of water (100 v/v)
B5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 1500 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
300 μl of polyethylene glycol 1500 BCC (50 w/v)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
550 μl of water (100 v/v)
B6
  • polyethylene glycol 1500 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400 μl of polyethylene glycol 1500 BCC (50 w/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of water (100 v/v)
B7
  • polyethylene glycol 1500 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
400 μl of polyethylene glycol 1500 BCC (50 w/v)
500 μl of water (100 v/v)
B8
  • ADA BCC 0.1 M pH 6.5
  • polyethylene glycol 1500 BCC 20 w/v -
  • potassium chloride BCC 0.2 M -
pH 6.5
90 μl of ADA BCC pH 7.5 (0.5 M)
109 μl of ADA BCC pH 5.5 (0.5 M)
200 μl of potassium chloride BCC (1 M)
400 μl of polyethylene glycol 1500 BCC (50 w/v)
200 μl of water (100 v/v)
B9
  • polyethylene glycol 1500 BCC 20 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
75 μl of potassium thiocyanate BCC (2 M)
36 μl of sodium MOPS BCC pH 8.2 (0.5 M)
63 μl of sodium MOPS BCC pH 6.2 (0.5 M)
400 μl of polyethylene glycol 1500 BCC (50 w/v)
425 μl of water (100 v/v)
B10
  • polyethylene glycol 1500 BCC 25 w/v -
NA
500 μl of polyethylene glycol 1500 BCC (50 w/v)
500 μl of water (100 v/v)
B11
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 1500 BCC 25 w/v -
  • magnesium sulfate BCC 0.2 M -
pH 9.5
200 μl of magnesium sulfate BCC (1 M)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
500 μl of polyethylene glycol 1500 BCC (50 w/v)
175 μl of water (100 v/v)
B12
  • polyethylene glycol 1500 BCC 30 w/v -
NA
600 μl of polyethylene glycol 1500 BCC (50 w/v)
400 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 1500 BCC 30 w/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
100 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
200 μl of ammonium sulfate BCC (1 M)
600 μl of polyethylene glycol 1500 BCC (50 w/v)
100 μl of water (100 v/v)
C2
  • ethanol BCC 10 v/v -
  • polyethylene glycol monomethyl ether 2000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of ethanol BCC (100 v/v)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600 μl of water (100 v/v)
C3
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 15 w/v -
pH 8.5
150 μl of sodium chloride BCC (1 M)
300 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
450 μl of water (100 v/v)
C4
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • polyethylene glycol 2000 BCC 16 w/v -
pH 7.5
320 μl of polyethylene glycol 2000 BCC (50 w/v)
300 μl of magnesium nitrate BCC (1 M)
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
280 μl of water (100 v/v)
C5
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 18 w/v -
pH 6.5
360 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
540 μl of water (100 v/v)
C6
  • lithium sulfate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
100 μl of lithium sulfate BCC (1 M)
400 μl of water (100 v/v)
C7
  • trisodium citrate-citric acid BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6.5
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
96 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
4 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of water (100 v/v)
C8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol 2000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400 μl of polyethylene glycol 2000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
50 μl of calcium chloride BCC (1 M)
450 μl of water (100 v/v)
C9
  • tris chloride BCC 0.1 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 7
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of tris chloride BCC pH 7 (1 M)
500 μl of water (100 v/v)
C10
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 7
200 μl of sodium chloride BCC (1 M)
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
C11
  • ammonium nitrate BCC 0.3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 2000 BCC 20 w/v -
pH 7.5
400 μl of polyethylene glycol 2000 BCC (50 w/v)
300 μl of ammonium nitrate BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of water (100 v/v)
C12
  • tris chloride BCC 0.1 M pH 8.5
  • triammonium citrate BCC 0.2 M -
  • polyethylene glycol 2000 BCC 20 w/v -
pH 8.5
400 μl of polyethylene glycol 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
80 μl of triammonium citrate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
420 μl of water (100 v/v)
D1
  • nickel(II) chloride BCC 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 8.5
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
10 μl of nickel(II) chloride BCC (1 M)
490 μl of water (100 v/v)
D2
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
  • trimethylamine N-oxide BCC 0.2 M -
pH 8.5
200 μl of trimethylamine N-oxide BCC (1 M)
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
D3
  • potassium bromide BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 5.5
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200 μl of potassium bromide BCC (1 M)
40 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250 μl of water (100 v/v)
D4
  • potassium thiocyanate BCC 0.2 M -
  • sodium cacodylate BCC 0.05 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of potassium thiocyanate BCC (2 M)
15 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
350 μl of water (100 v/v)
D5
  • lithium sulfate BCC 0.2 M -
  • sodium MES BCC 0.05 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6.5
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
13 μl of sodium MES BCC pH 5.2 (1 M)
36 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of lithium sulfate BCC (1 M)
250 μl of water (100 v/v)
D6
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6.5
200 μl of sodium chloride BCC (1 M)
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of water (100 v/v)
D7
  • magnesium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
9 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of magnesium chloride BCC (1 M)
250 μl of water (100 v/v)
D8
  • sodium acetate BCC 0.3 M -
  • sodium HEPES BCC 0.05 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7.5
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of sodium acetate BCC (1 M)
150 μl of water (100 v/v)
D9
  • calcium acetate BCC 0.2 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 8
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200 μl of calcium acetate BCC (1 M)
27 μl of tris chloride BCC pH 7 (1 M)
22 μl of tris chloride BCC pH 9 (1 M)
250 μl of water (100 v/v)
D10
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 8
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
27 μl of tris chloride BCC pH 7 (1 M)
22 μl of tris chloride BCC pH 9 (1 M)
200 μl of sodium formate BCC (4 M)
250 μl of water (100 v/v)
D11
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
200 μl of sodium chloride BCC (1 M)
100 μl of sodium bicine BCC pH 9.4 (1 M)
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200 μl of water (100 v/v)
D12
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 27 w/v -
pH 6.5
540 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
360 μl of water (100 v/v)
E1
  • polyethylene glycol monomethyl ether 2000 BCC 28 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
560 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
340 μl of water (100 v/v)
E2
  • potassium bromide BCC 0.15 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
150 μl of potassium bromide BCC (1 M)
250 μl of water (100 v/v)
E3
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50 μl of potassium thiocyanate BCC (2 M)
350 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200 μl of ammonium sulfate BCC (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
100 μl of water (100 v/v)
E5
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol 2000 BCC 30 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
600 μl of polyethylene glycol 2000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
50 μl of potassium thiocyanate BCC (2 M)
250 μl of water (100 v/v)
E6
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 7.5
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
E7
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 8.5
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
E8
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 36 w/v -
pH 6.5
720 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
180 μl of water (100 v/v)
E9
  • sodium cacodylate BCC 0.05 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 45 w/v -
pH 6.5
900 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
32 μl of sodium cacodylate BCC pH 7.3 (1 M)
17 μl of sodium cacodylate BCC pH 5.3 (1 M)
50 μl of water (100 v/v)
E10
  • polyethylene glycol 3000 BCC 10 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200 μl of polyethylene glycol 3000 BCC (50 w/v)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20 μl of zinc acetate BCC (1 M)
680 μl of water (100 v/v)
E11
  • polyethylene glycol 3000 BCC 10 w/v -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
200 μl of polyethylene glycol 3000 BCC (50 w/v)
20 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
700 μl of water (100 v/v)
E12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3000 BCC 10 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200 μl of polyethylene glycol 3000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
500 μl of water (100 v/v)
F1
  • imidazole BCC 0.1 M pH 8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3000 BCC 10 w/v -
pH 8
200 μl of polyethylene glycol 3000 BCC (50 w/v)
200 μl of lithium sulfate BCC (1 M)
100 μl of imidazole BCC pH 8 (1 M)
500 μl of water (100 v/v)
F2
  • cadmium chloride BCC 0.005 M -
  • cobalt chloride BCC 0.005 M -
  • magnesium chloride BCC 0.005 M -
  • nickel(II) chloride BCC 0.005 M -
  • polyethylene glycol 3000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of cadmium chloride BCC (0.1 M)
50 μl of cobalt chloride BCC (0.1 M)
240 μl of polyethylene glycol 3000 BCC (50 w/v)
5 μl of nickel(II) chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
5 μl of magnesium chloride BCC (1 M)
550 μl of water (100 v/v)
F3
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 3000 BCC 15 w/v -
  • sodium chloride BCC 0.2 M -
pH 4.2
200 μl of sodium chloride BCC (1 M)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
300 μl of polyethylene glycol 3000 BCC (50 w/v)
300 μl of water (100 v/v)
F4
  • polyethylene glycol 3000 BCC 20 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
400 μl of polyethylene glycol 3000 BCC (50 w/v)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500 μl of water (100 v/v)
F5
  • polyethylene glycol 3000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400 μl of polyethylene glycol 3000 BCC (50 w/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of water (100 v/v)
F6
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 3000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
400 μl of polyethylene glycol 3000 BCC (50 w/v)
200 μl of calcium acetate BCC (1 M)
100 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
F7
  • polyethylene glycol 3000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200 μl of sodium chloride BCC (1 M)
400 μl of polyethylene glycol 3000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
F8
  • magnesium nitrate BCC 0.02 M -
  • polyethylene glycol 3000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400 μl of polyethylene glycol 3000 BCC (50 w/v)
20 μl of magnesium nitrate BCC (1 M)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
480 μl of water (100 v/v)
F9
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 3000 BCC 20 w/v -
pH 9.5
400 μl of polyethylene glycol 3000 BCC (50 w/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
475 μl of water (100 v/v)
F10
  • polyethylene glycol 3000 BCC 30 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
200 μl of sodium chloride BCC (1 M)
600 μl of polyethylene glycol 3000 BCC (50 w/v)
100 μl of tris chloride BCC pH 7 (1 M)
100 μl of water (100 v/v)
F11
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 3000 BCC 30 w/v -
pH 9.5
600 μl of polyethylene glycol 3000 BCC (50 w/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
275 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • proline BCC 0.2 M -
pH 7.5
200 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of proline BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of water (100 v/v)
G1
  • magnesium formate BCC 0.1 M -
  • polyethylene glycol 3350 BCC 15 w/v -
NA
300 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of magnesium formate BCC (1 M)
600 μl of water (100 v/v)
G2
  • polyethylene glycol 3350 BCC 15 w/v -
  • sodium succinate BCC 0.1 M pH 7
pH 7
100 μl of sodium succinate BCC pH 7 (1 M)
300 μl of polyethylene glycol 3350 BCC (50 w/v)
600 μl of water (100 v/v)
G3
  • polyethylene glycol 3350 BCC 18 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • sodium MES BCC 0.05 M pH 6
pH 6
360 μl of polyethylene glycol 3350 BCC (50 w/v)
75 μl of potassium thiocyanate BCC (2 M)
30 μl of sodium MES BCC pH 5.2 (1 M)
19 μl of sodium MES BCC pH 7.2 (1 M)
515 μl of water (100 v/v)
G4
  • polyethylene glycol 3350 BCC 20 w/v -
  • DL-malic acid BCC 0.15 M pH 7
pH 7
400 μl of polyethylene glycol 3350 BCC (50 w/v)
150 μl of DL-malic acid BCC pH 7 (1 M)
450 μl of water (100 v/v)
G5
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
40 μl of ammonium acetate BCC (5 M)
560 μl of water (100 v/v)
G6
  • ammonium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
G7
  • ammonium dihydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
G8
  • ammonium fluoride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium fluoride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
G9
  • polyethylene glycol 3350 BCC 20 w/v -
  • ammonium formate BCC 0.2 M -
NA
200 μl of ammonium formate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
G10
  • ammonium iodide BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of ammonium iodide BCC (1 M)
400 μl of water (100 v/v)
G11
  • ammonium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of ammonium nitrate BCC (1 M)
400 μl of water (100 v/v)
G12
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
H1
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of calcium acetate BCC (1 M)
400 μl of water (100 v/v)
H2
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of calcium chloride BCC (1 M)
400 μl of water (100 v/v)
H3
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of diammonium hydrogen citrate BCC (2 M)
500 μl of water (100 v/v)
H4
  • diammonium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
57 μl of diammonium hydrogen phosphate BCC (3.5 M)
542 μl of water (100 v/v)
H5
  • polyethylene glycol 3350 BCC 20 w/v -
  • diammonium tartrate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of diammonium tartrate BCC (2 M)
500 μl of water (100 v/v)
H6
  • dipotassium hydrogen phosphate BCC 0.2 M pH 9.2
  • polyethylene glycol 3350 BCC 20 w/v -
pH 9.2
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
400 μl of water (100 v/v)
H7
  • disodium hydrogen phosphate BCC 0.2 M pH 9.3
  • polyethylene glycol 3350 BCC 20 w/v -
pH 9.3
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
200 μl of water (100 v/v)
H8
  • polyethylene glycol 3350 BCC 20 w/v -
  • disodium tartrate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of disodium tartrate BCC (1 M)
400 μl of water (100 v/v)
H9
  • lithium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
80 μl of lithium acetate BCC (2.5 M)
520 μl of water (100 v/v)
H10
  • lithium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of lithium chloride BCC (1 M)
400 μl of water (100 v/v)
H11
  • lithium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of lithium nitrate BCC (1 M)
400 μl of water (100 v/v)
H12
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of lithium sulfate BCC (1 M)
400 μl of water (100 v/v)