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Supplemental Information

Screen Description

c3_2

Created 2025-07-17.
Peggy: Low-Mid MW, diverse buffers, pH range and salts.

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c3_2

  • Z0002786 Lot 2 (1500.0 µl)
  • Z0002787 Lot 3 (1500.0 µl)
  • Z0002637 Lot 4 (0.0 µl)
  • Z0002638 Lot 1 (0.0 µl)

Screen Structure

c3_2

Well Count 96
Number of distinct ingredients 63
Internal diversity (score mean dist) 0.934

Screen Composition

2025-08-15T13:32:49.907133 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type c3_2
Buffer 73
Organic (non-volatile) 3
Organic (volatile) 1
Polymer 104
Salt 76

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 1000 BCC 10 w/v -
  • polyethylene glycol 8000 BCC 10 w/v -
NA
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
600.0 μl of water (100 v/v)
A2
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium acetate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 5.5
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
300.0 μl of sodium acetate BCC (1 M)
40.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250.0 μl of water (100 v/v)
A3
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium cacodylate BCC 0.05 M pH 6
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6
200.0 μl of lithium sulfate BCC (1 M)
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.6 μl of sodium cacodylate BCC pH 5.3 (1 M)
15.4 μl of sodium cacodylate BCC pH 7.3 (1 M)
350.0 μl of water (100 v/v)
A4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium MES BCC 0.05 M pH 6.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
350.0 μl of water (100 v/v)
A5
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium HEPES BCC 0.05 M pH 7
  • potassium bromide BCC 0.2 M -
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of potassium bromide BCC (1 M)
40.9 μl of sodium HEPES BCC pH 6.5 (1 M)
9.1 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
A6
  • polyethylene glycol 1000 BCC 10 w/v -
  • potassium thiocyanate BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7.5
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of potassium thiocyanate BCC (2 M)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
450.0 μl of water (100 v/v)
A7
  • polyethylene glycol 1000 BCC 10 w/v -
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of sodium formate BCC (4 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
350.0 μl of water (100 v/v)
A8
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 1000 BCC 10 w/v -
  • tris chloride BCC 0.05 M pH 8.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8.5
200.0 μl of calcium acetate BCC (1 M)
200.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
10.6 μl of tris chloride BCC pH 7 (1 M)
39.4 μl of tris chloride BCC pH 9 (1 M)
350.0 μl of water (100 v/v)
A9
  • lithium sulfate BCC 0.2 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 1000 BCC 20 w/v -
pH 4.2
200.0 μl of lithium sulfate BCC (1 M)
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of water (100 v/v)
A10
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (1 M)
20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
300.0 μl of water (100 v/v)
A11
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
500.0 μl of water (100 v/v)
A12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 1000 BCC 20 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
300.0 μl of water (100 v/v)
B1
  • polyethylene glycol 1000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
B2
  • calcium acetate BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 1000 BCC 20 w/v -
pH 8
200.0 μl of calcium acetate BCC (1 M)
100.0 μl of imidazole BCC pH 8 (1 M)
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
300.0 μl of water (100 v/v)
B3
  • polyethylene glycol 1000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of polyethylene glycol 1000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
B4
  • polyethylene glycol 1000 BCC 25 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
500.0 μl of polyethylene glycol 1000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20.0 μl of zinc acetate BCC (1 M)
380.0 μl of water (100 v/v)
B5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 1500 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
300.0 μl of polyethylene glycol 1500 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
550.0 μl of water (100 v/v)
B6
  • polyethylene glycol 1500 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of polyethylene glycol 1500 BCC (50 w/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
B7
  • polyethylene glycol 1500 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of polyethylene glycol 1500 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
500.0 μl of water (100 v/v)
B8
  • ADA BCC 0.1 M pH 6.5
  • polyethylene glycol 1500 BCC 20 w/v -
  • potassium chloride BCC 0.2 M -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
400.0 μl of polyethylene glycol 1500 BCC (50 w/v)
200.0 μl of potassium chloride BCC (1 M)
200.0 μl of water (100 v/v)
B9
  • polyethylene glycol 1500 BCC 20 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
400.0 μl of polyethylene glycol 1500 BCC (50 w/v)
75.0 μl of potassium thiocyanate BCC (2 M)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
425.0 μl of water (100 v/v)
B10
  • polyethylene glycol 1500 BCC 25 w/v -
NA
500.0 μl of polyethylene glycol 1500 BCC (50 w/v)
500.0 μl of water (100 v/v)
B11
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 1500 BCC 25 w/v -
  • magnesium sulfate BCC 0.2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
200.0 μl of magnesium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 1500 BCC (50 w/v)
175.0 μl of water (100 v/v)
B12
  • polyethylene glycol 1500 BCC 30 w/v -
NA
600.0 μl of polyethylene glycol 1500 BCC (50 w/v)
400.0 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 1500 BCC 30 w/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 1500 BCC (50 w/v)
100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
100.0 μl of water (100 v/v)
C2
  • ethanol BCC 10 v/v -
  • polyethylene glycol monomethyl ether 2000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
100.0 μl of ethanol BCC (100 v/v)
200.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600.0 μl of water (100 v/v)
C3
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 15 w/v -
pH 8.5
300.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
150.0 μl of sodium chloride BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
450.0 μl of water (100 v/v)
C4
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • polyethylene glycol 2000 BCC 16 w/v -
pH 7.5
300.0 μl of magnesium nitrate BCC (1 M)
320.0 μl of polyethylene glycol 2000 BCC (50 w/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
280.0 μl of water (100 v/v)
C5
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 18 w/v -
pH 6.5
360.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
540.0 μl of water (100 v/v)
C6
  • lithium sulfate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6
100.0 μl of lithium sulfate BCC (1 M)
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
400.0 μl of water (100 v/v)
C7
  • trisodium citrate-citric acid BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6.5
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
96.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
4.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
C8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol 2000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
50.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 2000 BCC (50 w/v)
450.0 μl of water (100 v/v)
C9
  • tris chloride BCC 0.1 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 7
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
C10
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 7
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
300.0 μl of water (100 v/v)
C11
  • ammonium nitrate BCC 0.3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 2000 BCC 20 w/v -
pH 7.5
300.0 μl of ammonium nitrate BCC (1 M)
400.0 μl of polyethylene glycol 2000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C12
  • tris chloride BCC 0.1 M pH 8.5
  • triammonium citrate BCC 0.2 M -
  • polyethylene glycol 2000 BCC 20 w/v -
pH 8.5
400.0 μl of polyethylene glycol 2000 BCC (50 w/v)
80.0 μl of triammonium citrate BCC (2.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
420.0 μl of water (100 v/v)
D1
  • nickel(II) chloride BCC 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 8.5
10.0 μl of nickel(II) chloride BCC (1 M)
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)
D2
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
  • trimethylamine N-oxide BCC 0.2 M -
pH 8.5
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200.0 μl of trimethylamine N-oxide BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D3
  • potassium bromide BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 5.5
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200.0 μl of potassium bromide BCC (1 M)
40.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250.0 μl of water (100 v/v)
D4
  • potassium thiocyanate BCC 0.2 M -
  • sodium cacodylate BCC 0.05 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100.0 μl of potassium thiocyanate BCC (2 M)
34.6 μl of sodium cacodylate BCC pH 5.3 (1 M)
15.4 μl of sodium cacodylate BCC pH 7.3 (1 M)
350.0 μl of water (100 v/v)
D5
  • lithium sulfate BCC 0.2 M -
  • sodium MES BCC 0.05 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6.5
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
250.0 μl of water (100 v/v)
D6
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 6.5
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
200.0 μl of water (100 v/v)
D7
  • magnesium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
40.9 μl of sodium HEPES BCC pH 6.5 (1 M)
9.1 μl of sodium HEPES BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
D8
  • sodium acetate BCC 0.3 M -
  • sodium HEPES BCC 0.05 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7.5
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of sodium acetate BCC (1 M)
150.0 μl of water (100 v/v)
D9
  • calcium acetate BCC 0.2 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 8
200.0 μl of calcium acetate BCC (1 M)
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
250.0 μl of water (100 v/v)
D10
  • sodium formate BCC 0.8 M -
  • tris chloride BCC 0.05 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 8
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200.0 μl of sodium formate BCC (4 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
250.0 μl of water (100 v/v)
D11
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100.0 μl of sodium bicine BCC pH 9.4 (1 M)
200.0 μl of sodium chloride BCC (1 M)
200.0 μl of water (100 v/v)
D12
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 27 w/v -
pH 6.5
540.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
360.0 μl of water (100 v/v)
E1
  • polyethylene glycol monomethyl ether 2000 BCC 28 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
560.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
340.0 μl of water (100 v/v)
E2
  • potassium bromide BCC 0.15 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
150.0 μl of potassium bromide BCC (1 M)
250.0 μl of water (100 v/v)
E3
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50.0 μl of potassium thiocyanate BCC (2 M)
350.0 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
100.0 μl of water (100 v/v)
E5
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol 2000 BCC 30 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
600.0 μl of polyethylene glycol 2000 BCC (50 w/v)
50.0 μl of potassium thiocyanate BCC (2 M)
250.0 μl of water (100 v/v)
E6
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 7.5
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
E7
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 8.5
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
E8
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 36 w/v -
pH 6.5
720.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
180.0 μl of water (100 v/v)
E9
  • sodium cacodylate BCC 0.05 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 45 w/v -
pH 6.5
900.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
17.4 μl of sodium cacodylate BCC pH 5.3 (1 M)
32.6 μl of sodium cacodylate BCC pH 7.3 (1 M)
50.0 μl of water (100 v/v)
E10
  • polyethylene glycol 3000 BCC 10 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of polyethylene glycol 3000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20.0 μl of zinc acetate BCC (1 M)
680.0 μl of water (100 v/v)
E11
  • polyethylene glycol 3000 BCC 10 w/v -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
200.0 μl of polyethylene glycol 3000 BCC (50 w/v)
20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
700.0 μl of water (100 v/v)
E12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3000 BCC 10 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
200.0 μl of polyethylene glycol 3000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
500.0 μl of water (100 v/v)
F1
  • imidazole BCC 0.1 M pH 8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3000 BCC 10 w/v -
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
200.0 μl of lithium sulfate BCC (1 M)
200.0 μl of polyethylene glycol 3000 BCC (50 w/v)
500.0 μl of water (100 v/v)
F2
  • cadmium chloride BCC 0.005 M -
  • cobalt chloride BCC 0.005 M -
  • magnesium chloride BCC 0.005 M -
  • nickel(II) chloride BCC 0.005 M -
  • polyethylene glycol 3000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of cadmium chloride BCC (0.1 M)
50.0 μl of cobalt chloride BCC (0.1 M)
5.0 μl of magnesium chloride BCC (1 M)
5.0 μl of nickel(II) chloride BCC (1 M)
240.0 μl of polyethylene glycol 3000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
F3
  • phosphate-citrate BCC 0.1 M pH 4.2
  • polyethylene glycol 3000 BCC 15 w/v -
  • sodium chloride BCC 0.2 M -
pH 4.2
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
300.0 μl of polyethylene glycol 3000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (1 M)
300.0 μl of water (100 v/v)
F4
  • polyethylene glycol 3000 BCC 20 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of water (100 v/v)
F5
  • polyethylene glycol 3000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
F6
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 3000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
200.0 μl of calcium acetate BCC (1 M)
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
300.0 μl of water (100 v/v)
F7
  • polyethylene glycol 3000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
300.0 μl of water (100 v/v)
F8
  • magnesium nitrate BCC 0.02 M -
  • polyethylene glycol 3000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
20.0 μl of magnesium nitrate BCC (1 M)
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
480.0 μl of water (100 v/v)
F9
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 3000 BCC 20 w/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
475.0 μl of water (100 v/v)
F10
  • polyethylene glycol 3000 BCC 30 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
600.0 μl of polyethylene glycol 3000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (1 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
100.0 μl of water (100 v/v)
F11
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 3000 BCC 30 w/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
600.0 μl of polyethylene glycol 3000 BCC (50 w/v)
275.0 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • proline BCC 0.2 M -
pH 7.5
200.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of proline BCC (1 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G1
  • magnesium formate BCC 0.1 M -
  • polyethylene glycol 3350 BCC 15 w/v -
NA
100.0 μl of magnesium formate BCC (1 M)
300.0 μl of polyethylene glycol 3350 BCC (50 w/v)
600.0 μl of water (100 v/v)
G2
  • polyethylene glycol 3350 BCC 15 w/v -
  • sodium succinate BCC 0.1 M pH 7
pH 7
300.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of sodium succinate BCC pH 7 (1 M)
600.0 μl of water (100 v/v)
G3
  • polyethylene glycol 3350 BCC 18 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • sodium MES BCC 0.05 M pH 6
pH 6
360.0 μl of polyethylene glycol 3350 BCC (50 w/v)
75.0 μl of potassium thiocyanate BCC (2 M)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
515.0 μl of water (100 v/v)
G4
  • polyethylene glycol 3350 BCC 20 w/v -
  • DL-malic acid BCC 0.15 M pH 7
pH 7
150.0 μl of DL-malic acid BCC pH 7 (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
450.0 μl of water (100 v/v)
G5
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
40.0 μl of ammonium acetate BCC (5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
560.0 μl of water (100 v/v)
G6
  • ammonium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium chloride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
G7
  • ammonium dihydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
520.0 μl of water (100 v/v)
G8
  • ammonium fluoride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium fluoride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
G9
  • polyethylene glycol 3350 BCC 20 w/v -
  • ammonium formate BCC 0.2 M -
NA
200.0 μl of ammonium formate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
G10
  • ammonium iodide BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium iodide BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
G11
  • ammonium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium nitrate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
G12
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H1
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of calcium acetate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H2
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H3
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
100.0 μl of diammonium hydrogen citrate BCC (2 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
500.0 μl of water (100 v/v)
H4
  • diammonium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
57.1 μl of diammonium hydrogen phosphate BCC (3.5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
542.9 μl of water (100 v/v)
H5
  • polyethylene glycol 3350 BCC 20 w/v -
  • diammonium tartrate BCC 0.2 M -
NA
100.0 μl of diammonium tartrate BCC (2 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
500.0 μl of water (100 v/v)
H6
  • dipotassium hydrogen phosphate BCC 0.2 M pH 9.2
  • polyethylene glycol 3350 BCC 20 w/v -
pH 9.2
200.0 μl of dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H7
  • disodium hydrogen phosphate BCC 0.2 M pH 9.3
  • polyethylene glycol 3350 BCC 20 w/v -
pH 9.3
400.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of water (100 v/v)
H8
  • polyethylene glycol 3350 BCC 20 w/v -
  • disodium tartrate BCC 0.2 M -
NA
200.0 μl of disodium tartrate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H9
  • lithium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80.0 μl of lithium acetate BCC (2.5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
520.0 μl of water (100 v/v)
H10
  • lithium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of lithium chloride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H11
  • lithium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of lithium nitrate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H12
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of lithium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)