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Supplemental Information

Screen Description

ProPlex_C3

BCC Core Screens / ProPlex_C3
Created 2025-03-07.
Based on Molecular Dimensions ProPlex screen, made up by C3.

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ProPlex_C3

  • Z0002521 Lot 2 (1500.0 µl)
  • Z0002799 Lot 3 (1500.0 µl)
  • Z0002800 Lot 4 (1500.0 µl)
  • Z0002520 Lot 1 (0.0 µl)

Screen Structure

ProPlex_C3

Well Count 96
Number of distinct ingredients 42
Internal diversity (score mean dist) 0.923

Screen Composition

2025-08-15T11:09:52.017474 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type ProPlex_C3
Buffer 96
Organic (non-volatile) 5
Organic (volatile) 6
Polymer 68
Salt 77

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
ProPlex_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 350 BCC 25 v/v -
pH 8
312.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
587.5 μl of water (100 v/v)
A2
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 400 BCC 15 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
100.0 μl of calcium acetate BCC (1 M)
187.5 μl of polyethylene glycol 400 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
612.5 μl of water (100 v/v)
A3
  • lithium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of lithium chloride BCC (1 M)
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
A4
  • polyethylene glycol 400 BCC 25 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
312.5 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
587.5 μl of water (100 v/v)
A5
  • polyethylene glycol monomethyl ether 550 BCC 15 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
176.5 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
723.5 μl of water (100 v/v)
A6
  • polyethylene glycol 1000 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 1000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (1 M)
22.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
11.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
266.7 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 0.1 M -
  • polyethylene glycol 1500 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 1500 BCC (50 w/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
A8
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of ammonium sulfate BCC (1 M)
200.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of water (100 v/v)
A9
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6
400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
300.0 μl of water (100 v/v)
A10
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 15 w/v -
pH 8
300.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100.0 μl of potassium chloride BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
A11
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7.5
500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
A12
  • polyethylene glycol 4000 BCC 5 w/v -
  • sodium acetate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
250.0 μl of polyethylene glycol 4000 BCC (20 w/v)
200.0 μl of sodium acetate BCC (1 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
450.0 μl of water (100 v/v)
B1
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 5 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of lithium sulfate BCC (1 M)
250.0 μl of polyethylene glycol 4000 BCC (20 w/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
450.0 μl of water (100 v/v)
B2
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
100.0 μl of calcium acetate BCC (1 M)
500.0 μl of polyethylene glycol 4000 BCC (20 w/v)
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
300.0 μl of water (100 v/v)
B3
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium acetate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
500.0 μl of polyethylene glycol 4000 BCC (20 w/v)
200.0 μl of sodium acetate BCC (1 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
200.0 μl of water (100 v/v)
B4
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 4000 BCC (20 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
200.0 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 4000 BCC (20 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B6
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
125.0 μl of 2-propanol BCC (80 v/v)
500.0 μl of polyethylene glycol 4000 BCC (20 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
275.0 μl of water (100 v/v)
B7
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4
pH 4
40.0 μl of ammonium acetate BCC (5 M)
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
6.0 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
94.0 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
110.0 μl of water (100 v/v)
B8
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
100.0 μl of magnesium chloride BCC (1 M)
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
50.0 μl of water (100 v/v)
B9
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
69.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
150.0 μl of water (100 v/v)
B10
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
150.0 μl of ammonium sulfate BCC (1 M)
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
B11
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
B12
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
100.0 μl of magnesium chloride BCC (1 M)
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
150.0 μl of ammonium sulfate BCC (1 M)
750.0 μl of polyethylene glycol 4000 BCC (20 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
C2
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
56.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
44.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
C3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
40.0 μl of ammonium acetate BCC (5 M)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
460.0 μl of water (100 v/v)
C4
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
200.0 μl of lithium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
C5
  • polyethylene glycol 4000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
150.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
C7
  • 2-propanol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
250.0 μl of 2-propanol BCC (80 v/v)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250.0 μl of water (100 v/v)
C8
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
C9
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium cacodylate BCC 0.1 M pH 5.5
pH 5.5
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
94.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
5.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
400.0 μl of water (100 v/v)
C10
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
150.0 μl of ammonium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
90.0 μl of sodium MES BCC pH 5.2 (1 M)
10.0 μl of sodium MES BCC pH 7.2 (1 M)
250.0 μl of water (100 v/v)
C11
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
400.0 μl of water (100 v/v)
C12
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium MES BCC 0.1 M pH 6.5
  • potassium iodide BCC 0.2 M -
pH 6.5
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
40.0 μl of potassium iodide BCC (5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
360.0 μl of water (100 v/v)
D1
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
200.0 μl of water (100 v/v)
D2
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium MES BCC 0.1 M pH 6.5
  • 1-propanol BCC 12 v/v -
pH 6.5
150.0 μl of 1-propanol BCC (80 v/v)
500.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
250.0 μl of water (100 v/v)
D3
  • polyethylene glycol monomethyl ether 5000 BCC 15 w/v -
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
750.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
100.0 μl of potassium chloride BCC (1 M)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D5
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
100.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 6000 BCC (20 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
400.0 μl of water (100 v/v)
D6
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of polyethylene glycol 6000 BCC (20 w/v)
150.0 μl of sodium chloride BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
350.0 μl of water (100 v/v)
D7
  • polyethylene glycol 6000 BCC 15 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
750.0 μl of polyethylene glycol 6000 BCC (20 w/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
150.0 μl of water (100 v/v)
D8
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
40.0 μl of magnesium acetate BCC (2.5 M)
750.0 μl of polyethylene glycol 6000 BCC (20 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
110.0 μl of water (100 v/v)
D9
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
750.0 μl of polyethylene glycol 6000 BCC (20 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
100.0 μl of water (100 v/v)
D10
  • polyethylene glycol 6000 BCC 15 w/v -
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
750.0 μl of polyethylene glycol 6000 BCC (20 w/v)
100.0 μl of potassium chloride BCC (1 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of water (100 v/v)
D11
  • polyethylene glycol 6000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
750.0 μl of polyethylene glycol 6000 BCC (20 w/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
150.0 μl of water (100 v/v)
D12
  • polyethylene glycol 6000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
E1
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 8000 BCC 8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
40.0 μl of magnesium acetate BCC (2.5 M)
400.0 μl of polyethylene glycol 8000 BCC (20 w/v)
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
460.0 μl of water (100 v/v)
E2
  • trisodium citrate-citric acid BCC 0.1 M pH 5
  • polyethylene glycol 8000 BCC 8 w/v -
pH 5
400.0 μl of polyethylene glycol 8000 BCC (20 w/v)
68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
E3
  • sodium cacodylate BCC 0.1 M pH 6
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 8 w/v -
pH 6
400.0 μl of polyethylene glycol 8000 BCC (20 w/v)
69.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
200.0 μl of sodium chloride BCC (1 M)
300.0 μl of water (100 v/v)
E4
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 8 w/v -
pH 7
400.0 μl of polyethylene glycol 8000 BCC (20 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 8 w/v -
pH 8
400.0 μl of polyethylene glycol 8000 BCC (20 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
E6
  • calcium acetate BCC 0.1 M -
  • sodium cacodylate BCC 0.1 M pH 5.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 5.5
100.0 μl of calcium acetate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (20 w/v)
94.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
5.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
200.0 μl of water (100 v/v)
E7
  • polyethylene glycol 8000 BCC 12 w/v -
  • sodium dihydrogen phosphate BCC 0.1 M -
NA
600.0 μl of polyethylene glycol 8000 BCC (20 w/v)
100.0 μl of sodium dihydrogen phosphate BCC (1 M)
300.0 μl of water (100 v/v)
E8
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 8000 BCC 12 w/v -
  • sodium MOPS BCC 0.1 M pH 7.5
pH 7.5
40.0 μl of magnesium acetate BCC (2.5 M)
600.0 μl of polyethylene glycol 8000 BCC (20 w/v)
59.4 μl of sodium MOPS BCC pH 6.2 (0.5 M)
140.6 μl of sodium MOPS BCC pH 8.2 (0.5 M)
160.0 μl of water (100 v/v)
E9
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 7.5
600.0 μl of polyethylene glycol 8000 BCC (20 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
100.0 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 8.5
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (20 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 5
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
E12
  • ammonium sulfate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6.5
200.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
F1
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 20 w/v -
pH 7
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
F2
  • lithium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8
200.0 μl of lithium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F3
  • magnesium acetate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 10000 BCC 10 w/v -
pH 6.5
40.0 μl of magnesium acetate BCC (2.5 M)
333.3 μl of polyethylene glycol 10000 BCC (30 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
526.7 μl of water (100 v/v)
F4
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 10000 BCC 18 w/v -
pH 7
600.0 μl of polyethylene glycol 10000 BCC (30 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F5
  • polyethylene glycol 20000 BCC 8 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
100.0 μl of sodium chloride BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
480.0 μl of water (100 v/v)
F6
  • polyethylene glycol 20000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
600.0 μl of polyethylene glycol 20000 BCC (25 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F7
  • ammonium sulfate BCC 0.5 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
500.0 μl of ammonium sulfate BCC (1 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
400.0 μl of water (100 v/v)
F8
  • ammonium sulfate BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
285.7 μl of ammonium sulfate BCC (3.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
614.3 μl of water (100 v/v)
F9
  • ammonium sulfate BCC 1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
285.7 μl of ammonium sulfate BCC (3.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
614.3 μl of water (100 v/v)
F10
  • ammonium sulfate BCC 1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
285.7 μl of ammonium sulfate BCC (3.5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
614.3 μl of water (100 v/v)
F11
  • ammonium sulfate BCC 1.5 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
428.6 μl of ammonium sulfate BCC (3.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
471.4 μl of water (100 v/v)
F12
  • ammonium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
428.6 μl of ammonium sulfate BCC (3.5 M)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 1.5 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
428.6 μl of ammonium sulfate BCC (3.5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
471.4 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
571.4 μl of ammonium sulfate BCC (3.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
328.6 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
571.4 μl of ammonium sulfate BCC (3.5 M)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
571.4 μl of ammonium sulfate BCC (3.5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
328.6 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 1 M -
  • potassium chloride BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
285.7 μl of ammonium sulfate BCC (3.5 M)
333.3 μl of potassium chloride BCC (3 M)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
281.0 μl of water (100 v/v)
G6
  • sodium formate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium formate BCC (4 M)
400.0 μl of water (100 v/v)
G7
  • sodium formate BCC 3 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
750.0 μl of sodium formate BCC (4 M)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
150.0 μl of water (100 v/v)
G8
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.8 M pH 7.5
pH 7.5
42.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
224.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
733.3 μl of water (100 v/v)
G9
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1.3 M pH 7
pH 7
156.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
277.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
566.7 μl of water (100 v/v)
G10
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1.6 M pH 6.5
pH 6.5
352.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
181.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
466.7 μl of water (100 v/v)
G11
  • sodium acetate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
333.3 μl of sodium acetate BCC (3 M)
566.7 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7
  • trisodium citrate BCC 1 M -
pH 7
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
275.0 μl of water (100 v/v)
H1
  • sodium chloride BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
400.0 μl of sodium chloride BCC (5 M)
90.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
H2
  • lithium sulfate BCC 1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of lithium sulfate BCC (2.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
500.0 μl of water (100 v/v)
H3
  • lithium sulfate BCC 1.6 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
640.0 μl of lithium sulfate BCC (2.5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
260.0 μl of water (100 v/v)
H4
  • sodium malonate-malonic acid BCC 1.4 M pH 6
pH 6
411.8 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
588.2 μl of water (100 v/v)
H5
  • potassium sodium tartrate BCC 1.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
600.0 μl of potassium sodium tartrate BCC (2 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
H6
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
640.0 μl of magnesium sulfate BCC (2.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
260.0 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • polyethylene glycol 4000 BCC 2 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of polyethylene glycol 4000 BCC (20 w/v)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
650.0 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • calcium acetate BCC 0.05 M -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of calcium acetate BCC (1 M)
69.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
600.0 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • imidazole BCC 0.1 M pH 7
pH 7
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
52.4 μl of imidazole BCC pH 6 (1 M)
47.6 μl of imidazole BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
H10
  • 2-propanol BCC 10 v/v -
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol 4000 BCC 5 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-propanol BCC (80 v/v)
50.0 μl of magnesium chloride BCC (1 M)
250.0 μl of polyethylene glycol 4000 BCC (20 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
475.0 μl of water (100 v/v)
H11
  • 2-propanol BCC 25 v/v -
  • ammonium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
312.5 μl of 2-propanol BCC (80 v/v)
40.0 μl of ammonium acetate BCC (5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
547.5 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ethanol BCC 15 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
150.0 μl of ethanol BCC (100 v/v)
100.0 μl of sodium chloride BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
600.0 μl of water (100 v/v)