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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
ProPlex_C3
BCC Core Screens / ProPlex_C3
Created 2025-03-07.
Based on Molecular Dimensions ProPlex screen, made up by C3.
Export
ProPlex_C3
Barcodes
ProPlex_C3
- Z0002520
- Z0002521
- Z0002799
- Z0002800
Screen Structure
ProPlex_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 42 |
Internal diversity (score mean dist) | 0.923 |
Screen Composition
ingredient type | ProPlex_C3 |
---|---|
Buffer | 96 |
Organic (non-volatile) | 5 |
Organic (volatile) | 6 |
Polymer | 68 |
Salt | 77 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 312 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 45 μl of tris chloride BCC pH 9 (1 M) 587 μl of water (100 v/v) |
A2 |
| pH 6 | 100 μl of calcium acetate BCC (1 M) 187 μl of polyethylene glycol 400 BCC (80 v/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 612 μl of water (100 v/v) |
A3 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 100 μl of lithium chloride BCC (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 250 μl of polyethylene glycol 400 BCC (80 v/v) 550 μl of water (100 v/v) |
A4 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 312 μl of polyethylene glycol 400 BCC (80 v/v) 45 μl of tris chloride BCC pH 9 (1 M) 587 μl of water (100 v/v) |
A5 |
| pH 6.5 | 72 μl of sodium MES BCC pH 7.2 (1 M) 176 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 723 μl of water (100 v/v) |
A6 |
| pH 6.5 | 11 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 22 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 500 μl of polyethylene glycol 1000 BCC (50 w/v) 200 μl of sodium chloride BCC (1 M) 266 μl of water (100 v/v) |
A7 |
| pH 7.5 | 100 μl of ammonium sulfate BCC (1 M) 84 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 1500 BCC (50 w/v) 15 μl of tris chloride BCC pH 9 (1 M) 400 μl of water (100 v/v) |
A8 |
| pH 5.5 | 200 μl of ammonium sulfate BCC (1 M) 8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 200 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 500 μl of water (100 v/v) |
A9 |
| pH 6 | 400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 200 μl of sodium chloride BCC (1 M) 300 μl of water (100 v/v) |
A10 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 100 μl of potassium chloride BCC (1 M) 300 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 500 μl of water (100 v/v) |
A11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 400 μl of water (100 v/v) |
A12 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 200 μl of sodium acetate BCC (1 M) 250 μl of polyethylene glycol 4000 BCC (20 w/v) 450 μl of water (100 v/v) |
B1 |
| pH 7.5 | 84 μl of tris chloride BCC pH 7 (1 M) 200 μl of lithium sulfate BCC (1 M) 250 μl of polyethylene glycol 4000 BCC (20 w/v) 15 μl of tris chloride BCC pH 9 (1 M) 450 μl of water (100 v/v) |
B2 |
| pH 4.5 | 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 100 μl of calcium acetate BCC (1 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 500 μl of polyethylene glycol 4000 BCC (20 w/v) 300 μl of water (100 v/v) |
B3 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 200 μl of sodium acetate BCC (1 M) 500 μl of polyethylene glycol 4000 BCC (20 w/v) 200 μl of water (100 v/v) |
B4 |
| pH 6.5 | 72 μl of sodium MES BCC pH 7.2 (1 M) 500 μl of polyethylene glycol 4000 BCC (20 w/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 200 μl of sodium chloride BCC (1 M) 200 μl of water (100 v/v) |
B5 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 100 μl of magnesium chloride BCC (1 M) 500 μl of polyethylene glycol 4000 BCC (20 w/v) 300 μl of water (100 v/v) |
B6 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 125 μl of 2-propanol BCC (80 v/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 4000 BCC (20 w/v) 275 μl of water (100 v/v) |
B7 |
| pH 4 | 40 μl of ammonium acetate BCC (5 M) 94 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 6 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 110 μl of water (100 v/v) |
B8 |
| pH 5 | 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 100 μl of magnesium chloride BCC (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 50 μl of water (100 v/v) |
B9 |
| pH 6 | 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 150 μl of water (100 v/v) |
B10 |
| pH 6 | 150 μl of ammonium sulfate BCC (1 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 61 μl of sodium MES BCC pH 5.2 (1 M) |
B11 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 150 μl of water (100 v/v) |
B12 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 100 μl of magnesium chloride BCC (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 50 μl of water (100 v/v) |
C1 |
| pH 8 | 150 μl of ammonium sulfate BCC (1 M) 54 μl of tris chloride BCC pH 7 (1 M) 750 μl of polyethylene glycol 4000 BCC (20 w/v) 45 μl of tris chloride BCC pH 9 (1 M) |
C2 |
| pH 4.5 | 400 μl of polyethylene glycol 4000 BCC (50 w/v) 56 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 44 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500 μl of water (100 v/v) |
C3 |
| pH 5 | 40 μl of ammonium acetate BCC (5 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 460 μl of water (100 v/v) |
C4 |
| pH 6 | 400 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of lithium sulfate BCC (1 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 300 μl of water (100 v/v) |
C5 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 500 μl of water (100 v/v) |
C6 |
| pH 7 | 150 μl of ammonium sulfate BCC (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 350 μl of water (100 v/v) |
C7 |
| pH 5.6 | 400 μl of polyethylene glycol 4000 BCC (50 w/v) 82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 250 μl of 2-propanol BCC (80 v/v) 18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250 μl of water (100 v/v) |
C8 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 200 μl of sodium chloride BCC (1 M) 300 μl of water (100 v/v) |
C9 |
| pH 5.5 | 500 μl of polyethylene glycol 4000 BCC (50 w/v) 5 μl of sodium cacodylate BCC pH 7.3 (1 M) 94 μl of sodium cacodylate BCC pH 5.3 (1 M) 400 μl of water (100 v/v) |
C10 |
| pH 5.5 | 150 μl of ammonium sulfate BCC (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 10 μl of sodium MES BCC pH 7.2 (1 M) 90 μl of sodium MES BCC pH 5.2 (1 M) 250 μl of water (100 v/v) |
C11 |
| pH 6.5 | 500 μl of polyethylene glycol 4000 BCC (50 w/v) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 400 μl of water (100 v/v) |
C12 |
| pH 6.5 | 500 μl of polyethylene glycol 4000 BCC (50 w/v) 40 μl of potassium iodide BCC (5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 360 μl of water (100 v/v) |
D1 |
| pH 7.5 | 500 μl of polyethylene glycol 4000 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 200 μl of sodium chloride BCC (1 M) 200 μl of water (100 v/v) |
D2 |
| pH 6.5 | 500 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 150 μl of 1-propanol BCC (80 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 250 μl of water (100 v/v) |
D3 |
| pH 7 | 100 μl of potassium chloride BCC (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 750 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of water (100 v/v) |
D4 |
| pH 7.5 | 200 μl of ammonium sulfate BCC (1 M) 84 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 15 μl of tris chloride BCC pH 9 (1 M) 300 μl of water (100 v/v) |
D5 |
| pH 6 | 400 μl of polyethylene glycol 6000 BCC (20 w/v) 100 μl of magnesium chloride BCC (1 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 400 μl of water (100 v/v) |
D6 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 6000 BCC (20 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 150 μl of sodium chloride BCC (1 M) 350 μl of water (100 v/v) |
D7 |
| pH 5.5 | 750 μl of polyethylene glycol 6000 BCC (20 w/v) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 150 μl of water (100 v/v) |
D8 |
| pH 6.5 | 750 μl of polyethylene glycol 6000 BCC (20 w/v) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 40 μl of magnesium acetate BCC (2.5 M) 110 μl of water (100 v/v) |
D9 |
| pH 6.5 | 750 μl of polyethylene glycol 6000 BCC (20 w/v) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 100 μl of water (100 v/v) |
D10 |
| pH 7.5 | 100 μl of potassium chloride BCC (1 M) 750 μl of polyethylene glycol 6000 BCC (20 w/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of water (100 v/v) |
D11 |
| pH 7.5 | 84 μl of tris chloride BCC pH 7 (1 M) 750 μl of polyethylene glycol 6000 BCC (20 w/v) 15 μl of tris chloride BCC pH 9 (1 M) 150 μl of water (100 v/v) |
D12 |
| pH 8.5 | 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 6000 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of water (100 v/v) |
E1 |
| pH 4.5 | 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 40 μl of magnesium acetate BCC (2.5 M) 400 μl of polyethylene glycol 8000 BCC (20 w/v) 460 μl of water (100 v/v) |
E2 |
| pH 5 | 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of polyethylene glycol 8000 BCC (20 w/v) 500 μl of water (100 v/v) |
E3 |
| pH 6 | 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 200 μl of sodium chloride BCC (1 M) 400 μl of polyethylene glycol 8000 BCC (20 w/v) 300 μl of water (100 v/v) |
E4 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of polyethylene glycol 8000 BCC (20 w/v) 500 μl of water (100 v/v) |
E5 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 400 μl of polyethylene glycol 8000 BCC (20 w/v) 500 μl of water (100 v/v) |
E6 |
| pH 5.5 | 5 μl of sodium cacodylate BCC pH 7.3 (1 M) 100 μl of calcium acetate BCC (1 M) 94 μl of sodium cacodylate BCC pH 5.3 (1 M) 600 μl of polyethylene glycol 8000 BCC (20 w/v) 200 μl of water (100 v/v) |
E7 |
| NA | 100 μl of sodium dihydrogen phosphate BCC (1 M) 600 μl of polyethylene glycol 8000 BCC (20 w/v) 300 μl of water (100 v/v) |
E8 |
| pH 7.5 | 59 μl of sodium MOPS BCC pH 6.2 (0.5 M) 140 μl of sodium MOPS BCC pH 8.2 (0.5 M) 40 μl of magnesium acetate BCC (2.5 M) 600 μl of polyethylene glycol 8000 BCC (20 w/v) 160 μl of water (100 v/v) |
E9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 200 μl of sodium chloride BCC (1 M) 600 μl of polyethylene glycol 8000 BCC (20 w/v) 100 μl of water (100 v/v) |
E10 |
| pH 8.5 | 200 μl of ammonium sulfate BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 600 μl of polyethylene glycol 8000 BCC (20 w/v) 100 μl of water (100 v/v) |
E11 |
| pH 5 | 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 500 μl of water (100 v/v) |
E12 |
| pH 6.5 | 200 μl of ammonium sulfate BCC (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 300 μl of water (100 v/v) |
F1 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of water (100 v/v) |
F2 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 200 μl of lithium chloride BCC (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 300 μl of water (100 v/v) |
F3 |
| pH 6.5 | 333 μl of polyethylene glycol 10000 BCC (30 w/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 40 μl of magnesium acetate BCC (2.5 M) 526 μl of water (100 v/v) |
F4 |
| pH 7 | 600 μl of polyethylene glycol 10000 BCC (30 w/v) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of water (100 v/v) |
F5 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 320 μl of polyethylene glycol 20000 BCC (25 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 100 μl of sodium chloride BCC (1 M) 480 μl of water (100 v/v) |
F6 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 600 μl of polyethylene glycol 20000 BCC (25 w/v) 300 μl of water (100 v/v) |
F7 |
| pH 6.5 | 500 μl of ammonium sulfate BCC (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 400 μl of water (100 v/v) |
F8 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
F9 |
| pH 6.5 | 72 μl of sodium MES BCC pH 7.2 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 614 μl of water (100 v/v) |
F10 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 45 μl of tris chloride BCC pH 9 (1 M) 614 μl of water (100 v/v) |
F11 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
F12 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
G1 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 45 μl of tris chloride BCC pH 9 (1 M) 471 μl of water (100 v/v) |
G2 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
G3 |
| pH 7 | 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
G4 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 45 μl of tris chloride BCC pH 9 (1 M) 328 μl of water (100 v/v) |
G5 |
| pH 7 | 333 μl of potassium chloride BCC (3 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 281 μl of water (100 v/v) |
G6 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 500 μl of sodium formate BCC (4 M) 400 μl of water (100 v/v) |
G7 |
| pH 7.5 | 84 μl of tris chloride BCC pH 7 (1 M) 15 μl of tris chloride BCC pH 9 (1 M) 750 μl of sodium formate BCC (4 M) 150 μl of water (100 v/v) |
G8 |
| pH 7.5 | 224 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 42 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 733 μl of water (100 v/v) |
G9 |
| pH 7 | 277 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 156 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 566 μl of water (100 v/v) |
G10 |
| pH 6.5 | 181 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 352 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 466 μl of water (100 v/v) |
G11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 333 μl of sodium acetate BCC (3 M) 566 μl of water (100 v/v) |
G12 |
| pH 7 | 625 μl of trisodium citrate BCC (1.6 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 275 μl of water (100 v/v) |
H1 |
| pH 6 | 90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of sodium chloride BCC (5 M) 500 μl of water (100 v/v) |
H2 |
| pH 6.5 | 400 μl of lithium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 500 μl of water (100 v/v) |
H3 |
| pH 8 | 640 μl of lithium sulfate BCC (2.5 M) 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 260 μl of water (100 v/v) |
H4 |
| pH 6 | 411 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M) 588 μl of water (100 v/v) |
H5 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 600 μl of potassium sodium tartrate BCC (2 M) 45 μl of tris chloride BCC pH 9 (1 M) 300 μl of water (100 v/v) |
H6 |
| pH 6.5 | 640 μl of magnesium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 260 μl of water (100 v/v) |
H7 |
| pH 5 | 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of polyethylene glycol 4000 BCC (20 w/v) 650 μl of water (100 v/v) |
H8 |
| pH 6 | 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 50 μl of calcium acetate BCC (1 M) 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 600 μl of water (100 v/v) |
H9 |
| pH 7 | 52 μl of imidazole BCC pH 6 (1 M) 47 μl of imidazole BCC pH 8 (1 M) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of water (100 v/v) |
H10 |
| pH 6.5 | 125 μl of 2-propanol BCC (80 v/v) 50 μl of magnesium chloride BCC (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 250 μl of polyethylene glycol 4000 BCC (20 w/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 475 μl of water (100 v/v) |
H11 |
| pH 7.5 | 40 μl of ammonium acetate BCC (5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 312 μl of 2-propanol BCC (80 v/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 547 μl of water (100 v/v) |
H12 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 150 μl of ethanol BCC (100 v/v) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 45 μl of tris chloride BCC pH 9 (1 M) 100 μl of sodium chloride BCC (1 M) 600 μl of water (100 v/v) |