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Supplemental Information

Screen Description

ProPlex_C3

BCC Core Screens / ProPlex_C3
Created 2025-03-07.
Based on Molecular Dimensions ProPlex screen, made up by C3.

Export

Barcodes

ProPlex_C3

  • Z0002520
  • Z0002521
  • Z0002799
  • Z0002800

Screen Structure

ProPlex_C3

Well Count 96
Number of distinct ingredients 42
Internal diversity (score mean dist) 0.923

Screen Composition

2025-06-12T23:07:59.647524 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type ProPlex_C3
Buffer 96
Organic (non-volatile) 5
Organic (volatile) 6
Polymer 68
Salt 77

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
ProPlex_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 350 BCC 25 v/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
312 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
45 μl of tris chloride BCC pH 9 (1 M)
587 μl of water (100 v/v)
A2
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 400 BCC 15 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
100 μl of calcium acetate BCC (1 M)
187 μl of polyethylene glycol 400 BCC (80 v/v)
38 μl of sodium MES BCC pH 7.2 (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
612 μl of water (100 v/v)
A3
  • lithium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
100 μl of lithium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
550 μl of water (100 v/v)
A4
  • polyethylene glycol 400 BCC 25 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
312 μl of polyethylene glycol 400 BCC (80 v/v)
45 μl of tris chloride BCC pH 9 (1 M)
587 μl of water (100 v/v)
A5
  • polyethylene glycol monomethyl ether 550 BCC 15 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
176 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
723 μl of water (100 v/v)
A6
  • polyethylene glycol 1000 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.5
pH 6.5
11 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
22 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
500 μl of polyethylene glycol 1000 BCC (50 w/v)
200 μl of sodium chloride BCC (1 M)
266 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 0.1 M -
  • polyethylene glycol 1500 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
100 μl of ammonium sulfate BCC (1 M)
84 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 1500 BCC (50 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
400 μl of water (100 v/v)
A8
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
200 μl of ammonium sulfate BCC (1 M)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
200 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
500 μl of water (100 v/v)
A9
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
pH 6
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
38 μl of sodium MES BCC pH 7.2 (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
200 μl of sodium chloride BCC (1 M)
300 μl of water (100 v/v)
A10
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol monomethyl ether 2000 BCC 15 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
100 μl of potassium chloride BCC (1 M)
300 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
500 μl of water (100 v/v)
A11
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 25 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
500 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
400 μl of water (100 v/v)
A12
  • polyethylene glycol 4000 BCC 5 w/v -
  • sodium acetate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
200 μl of sodium acetate BCC (1 M)
250 μl of polyethylene glycol 4000 BCC (20 w/v)
450 μl of water (100 v/v)
B1
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 5 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
84 μl of tris chloride BCC pH 7 (1 M)
200 μl of lithium sulfate BCC (1 M)
250 μl of polyethylene glycol 4000 BCC (20 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
450 μl of water (100 v/v)
B2
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
100 μl of calcium acetate BCC (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of polyethylene glycol 4000 BCC (20 w/v)
300 μl of water (100 v/v)
B3
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium acetate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
200 μl of sodium acetate BCC (1 M)
500 μl of polyethylene glycol 4000 BCC (20 w/v)
200 μl of water (100 v/v)
B4
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
500 μl of polyethylene glycol 4000 BCC (20 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
200 μl of sodium chloride BCC (1 M)
200 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
100 μl of magnesium chloride BCC (1 M)
500 μl of polyethylene glycol 4000 BCC (20 w/v)
300 μl of water (100 v/v)
B6
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
125 μl of 2-propanol BCC (80 v/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 4000 BCC (20 w/v)
275 μl of water (100 v/v)
B7
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4
pH 4
40 μl of ammonium acetate BCC (5 M)
94 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
6 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
110 μl of water (100 v/v)
B8
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
100 μl of magnesium chloride BCC (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
50 μl of water (100 v/v)
B9
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
150 μl of water (100 v/v)
B10
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
150 μl of ammonium sulfate BCC (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
B11
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
150 μl of water (100 v/v)
B12
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
100 μl of magnesium chloride BCC (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
50 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
150 μl of ammonium sulfate BCC (1 M)
54 μl of tris chloride BCC pH 7 (1 M)
750 μl of polyethylene glycol 4000 BCC (20 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
C2
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
400 μl of polyethylene glycol 4000 BCC (50 w/v)
56 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
44 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of water (100 v/v)
C3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
40 μl of ammonium acetate BCC (5 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
460 μl of water (100 v/v)
C4
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
400 μl of polyethylene glycol 4000 BCC (50 w/v)
200 μl of lithium sulfate BCC (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
300 μl of water (100 v/v)
C5
  • polyethylene glycol 4000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
500 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
150 μl of ammonium sulfate BCC (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
350 μl of water (100 v/v)
C7
  • 2-propanol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
400 μl of polyethylene glycol 4000 BCC (50 w/v)
82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
250 μl of 2-propanol BCC (80 v/v)
18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250 μl of water (100 v/v)
C8
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
200 μl of sodium chloride BCC (1 M)
300 μl of water (100 v/v)
C9
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium cacodylate BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 4000 BCC (50 w/v)
5 μl of sodium cacodylate BCC pH 7.3 (1 M)
94 μl of sodium cacodylate BCC pH 5.3 (1 M)
400 μl of water (100 v/v)
C10
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
150 μl of ammonium sulfate BCC (1 M)
500 μl of polyethylene glycol 4000 BCC (50 w/v)
10 μl of sodium MES BCC pH 7.2 (1 M)
90 μl of sodium MES BCC pH 5.2 (1 M)
250 μl of water (100 v/v)
C11
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
500 μl of polyethylene glycol 4000 BCC (50 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
400 μl of water (100 v/v)
C12
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium MES BCC 0.1 M pH 6.5
  • potassium iodide BCC 0.2 M -
pH 6.5
500 μl of polyethylene glycol 4000 BCC (50 w/v)
40 μl of potassium iodide BCC (5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
360 μl of water (100 v/v)
D1
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 4000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
200 μl of sodium chloride BCC (1 M)
200 μl of water (100 v/v)
D2
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium MES BCC 0.1 M pH 6.5
  • 1-propanol BCC 12 v/v -
pH 6.5
500 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
150 μl of 1-propanol BCC (80 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
250 μl of water (100 v/v)
D3
  • polyethylene glycol monomethyl ether 5000 BCC 15 w/v -
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
100 μl of potassium chloride BCC (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
750 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
200 μl of ammonium sulfate BCC (1 M)
84 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
D5
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
400 μl of polyethylene glycol 6000 BCC (20 w/v)
100 μl of magnesium chloride BCC (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
400 μl of water (100 v/v)
D6
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 6000 BCC (20 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
150 μl of sodium chloride BCC (1 M)
350 μl of water (100 v/v)
D7
  • polyethylene glycol 6000 BCC 15 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
750 μl of polyethylene glycol 6000 BCC (20 w/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
150 μl of water (100 v/v)
D8
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
750 μl of polyethylene glycol 6000 BCC (20 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
40 μl of magnesium acetate BCC (2.5 M)
110 μl of water (100 v/v)
D9
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
750 μl of polyethylene glycol 6000 BCC (20 w/v)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
100 μl of water (100 v/v)
D10
  • polyethylene glycol 6000 BCC 15 w/v -
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100 μl of potassium chloride BCC (1 M)
750 μl of polyethylene glycol 6000 BCC (20 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of water (100 v/v)
D11
  • polyethylene glycol 6000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
84 μl of tris chloride BCC pH 7 (1 M)
750 μl of polyethylene glycol 6000 BCC (20 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
150 μl of water (100 v/v)
D12
  • polyethylene glycol 6000 BCC 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of water (100 v/v)
E1
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 8000 BCC 8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
40 μl of magnesium acetate BCC (2.5 M)
400 μl of polyethylene glycol 8000 BCC (20 w/v)
460 μl of water (100 v/v)
E2
  • trisodium citrate-citric acid BCC 0.1 M pH 5
  • polyethylene glycol 8000 BCC 8 w/v -
pH 5
68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 8000 BCC (20 w/v)
500 μl of water (100 v/v)
E3
  • sodium cacodylate BCC 0.1 M pH 6
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 8 w/v -
pH 6
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
200 μl of sodium chloride BCC (1 M)
400 μl of polyethylene glycol 8000 BCC (20 w/v)
300 μl of water (100 v/v)
E4
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 8 w/v -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
400 μl of polyethylene glycol 8000 BCC (20 w/v)
500 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 8 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (20 w/v)
500 μl of water (100 v/v)
E6
  • calcium acetate BCC 0.1 M -
  • sodium cacodylate BCC 0.1 M pH 5.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 5.5
5 μl of sodium cacodylate BCC pH 7.3 (1 M)
100 μl of calcium acetate BCC (1 M)
94 μl of sodium cacodylate BCC pH 5.3 (1 M)
600 μl of polyethylene glycol 8000 BCC (20 w/v)
200 μl of water (100 v/v)
E7
  • polyethylene glycol 8000 BCC 12 w/v -
  • sodium dihydrogen phosphate BCC 0.1 M -
NA
100 μl of sodium dihydrogen phosphate BCC (1 M)
600 μl of polyethylene glycol 8000 BCC (20 w/v)
300 μl of water (100 v/v)
E8
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 8000 BCC 12 w/v -
  • sodium MOPS BCC 0.1 M pH 7.5
pH 7.5
59 μl of sodium MOPS BCC pH 6.2 (0.5 M)
140 μl of sodium MOPS BCC pH 8.2 (0.5 M)
40 μl of magnesium acetate BCC (2.5 M)
600 μl of polyethylene glycol 8000 BCC (20 w/v)
160 μl of water (100 v/v)
E9
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
200 μl of sodium chloride BCC (1 M)
600 μl of polyethylene glycol 8000 BCC (20 w/v)
100 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 8.5
200 μl of ammonium sulfate BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
600 μl of polyethylene glycol 8000 BCC (20 w/v)
100 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 5
68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
500 μl of water (100 v/v)
E12
  • ammonium sulfate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6.5
200 μl of ammonium sulfate BCC (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
300 μl of water (100 v/v)
F1
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 20 w/v -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
500 μl of water (100 v/v)
F2
  • lithium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of lithium chloride BCC (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
F3
  • magnesium acetate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 10000 BCC 10 w/v -
pH 6.5
333 μl of polyethylene glycol 10000 BCC (30 w/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
40 μl of magnesium acetate BCC (2.5 M)
526 μl of water (100 v/v)
F4
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 10000 BCC 18 w/v -
pH 7
600 μl of polyethylene glycol 10000 BCC (30 w/v)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
300 μl of water (100 v/v)
F5
  • polyethylene glycol 20000 BCC 8 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
100 μl of sodium chloride BCC (1 M)
480 μl of water (100 v/v)
F6
  • polyethylene glycol 20000 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
600 μl of polyethylene glycol 20000 BCC (25 w/v)
300 μl of water (100 v/v)
F7
  • ammonium sulfate BCC 0.5 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
500 μl of ammonium sulfate BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
400 μl of water (100 v/v)
F8
  • ammonium sulfate BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
F9
  • ammonium sulfate BCC 1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
614 μl of water (100 v/v)
F10
  • ammonium sulfate BCC 1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
45 μl of tris chloride BCC pH 9 (1 M)
614 μl of water (100 v/v)
F11
  • ammonium sulfate BCC 1.5 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
F12
  • ammonium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 1.5 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
45 μl of tris chloride BCC pH 9 (1 M)
471 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
45 μl of tris chloride BCC pH 9 (1 M)
328 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 1 M -
  • potassium chloride BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
333 μl of potassium chloride BCC (3 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
281 μl of water (100 v/v)
G6
  • sodium formate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium formate BCC (4 M)
400 μl of water (100 v/v)
G7
  • sodium formate BCC 3 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
750 μl of sodium formate BCC (4 M)
150 μl of water (100 v/v)
G8
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.8 M pH 7.5
pH 7.5
224 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
42 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
733 μl of water (100 v/v)
G9
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1.3 M pH 7
pH 7
277 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
156 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
566 μl of water (100 v/v)
G10
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1.6 M pH 6.5
pH 6.5
181 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
352 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
466 μl of water (100 v/v)
G11
  • sodium acetate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
333 μl of sodium acetate BCC (3 M)
566 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7
  • trisodium citrate BCC 1 M -
pH 7
625 μl of trisodium citrate BCC (1.6 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
275 μl of water (100 v/v)
H1
  • sodium chloride BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of sodium chloride BCC (5 M)
500 μl of water (100 v/v)
H2
  • lithium sulfate BCC 1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400 μl of lithium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
500 μl of water (100 v/v)
H3
  • lithium sulfate BCC 1.6 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
640 μl of lithium sulfate BCC (2.5 M)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
260 μl of water (100 v/v)
H4
  • sodium malonate-malonic acid BCC 1.4 M pH 6
pH 6
411 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
588 μl of water (100 v/v)
H5
  • potassium sodium tartrate BCC 1.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
600 μl of potassium sodium tartrate BCC (2 M)
45 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
H6
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
640 μl of magnesium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
260 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • polyethylene glycol 4000 BCC 2 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100 μl of polyethylene glycol 4000 BCC (20 w/v)
650 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • calcium acetate BCC 0.05 M -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
50 μl of calcium acetate BCC (1 M)
250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
600 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • imidazole BCC 0.1 M pH 7
pH 7
52 μl of imidazole BCC pH 6 (1 M)
47 μl of imidazole BCC pH 8 (1 M)
500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
400 μl of water (100 v/v)
H10
  • 2-propanol BCC 10 v/v -
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol 4000 BCC 5 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
125 μl of 2-propanol BCC (80 v/v)
50 μl of magnesium chloride BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
250 μl of polyethylene glycol 4000 BCC (20 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
475 μl of water (100 v/v)
H11
  • 2-propanol BCC 25 v/v -
  • ammonium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
40 μl of ammonium acetate BCC (5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
312 μl of 2-propanol BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
547 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ethanol BCC 15 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
150 μl of ethanol BCC (100 v/v)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
45 μl of tris chloride BCC pH 9 (1 M)
100 μl of sodium chloride BCC (1 M)
600 μl of water (100 v/v)