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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
ProPlex_C3
BCC Core Screens / ProPlex_C3
Created 2025-03-07.
Based on Molecular Dimensions ProPlex screen, made up by C3.
Export
ProPlex_C3
Barcodes
ProPlex_C3
Screen Structure
ProPlex_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 42 |
Internal diversity (score mean dist) | 0.923 |
Screen Composition
ingredient type | ProPlex_C3 |
---|---|
Buffer | 96 |
Organic (non-volatile) | 5 |
Organic (volatile) | 6 |
Polymer | 68 |
Salt | 77 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8 | 312.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 587.5 μl of water (100 v/v) |
A2 |
| pH 6 | 100.0 μl of calcium acetate BCC (1 M) 187.5 μl of polyethylene glycol 400 BCC (80 v/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 612.5 μl of water (100 v/v) |
A3 |
| pH 7.5 | 100.0 μl of lithium chloride BCC (1 M) 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 550.0 μl of water (100 v/v) |
A4 |
| pH 8 | 312.5 μl of polyethylene glycol 400 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 587.5 μl of water (100 v/v) |
A5 |
| pH 6.5 | 176.5 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 723.5 μl of water (100 v/v) |
A6 |
| pH 6.5 | 500.0 μl of polyethylene glycol 1000 BCC (50 w/v) 200.0 μl of sodium chloride BCC (1 M) 22.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 11.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 266.7 μl of water (100 v/v) |
A7 |
| pH 7.5 | 100.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 1500 BCC (50 w/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 400.0 μl of water (100 v/v) |
A8 |
| pH 5.5 | 200.0 μl of ammonium sulfate BCC (1 M) 200.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of water (100 v/v) |
A9 |
| pH 6 | 400.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 200.0 μl of sodium chloride BCC (1 M) 300.0 μl of water (100 v/v) |
A10 |
| pH 8 | 300.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 100.0 μl of potassium chloride BCC (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 500.0 μl of water (100 v/v) |
A11 |
| pH 7.5 | 500.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
A12 |
| pH 5.5 | 250.0 μl of polyethylene glycol 4000 BCC (20 w/v) 200.0 μl of sodium acetate BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 450.0 μl of water (100 v/v) |
B1 |
| pH 7.5 | 200.0 μl of lithium sulfate BCC (1 M) 250.0 μl of polyethylene glycol 4000 BCC (20 w/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 450.0 μl of water (100 v/v) |
B2 |
| pH 4.5 | 100.0 μl of calcium acetate BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (20 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 300.0 μl of water (100 v/v) |
B3 |
| pH 5.5 | 500.0 μl of polyethylene glycol 4000 BCC (20 w/v) 200.0 μl of sodium acetate BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 200.0 μl of water (100 v/v) |
B4 |
| pH 6.5 | 500.0 μl of polyethylene glycol 4000 BCC (20 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 200.0 μl of sodium chloride BCC (1 M) 200.0 μl of water (100 v/v) |
B5 |
| pH 7.5 | 100.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (20 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
B6 |
| pH 7 | 125.0 μl of 2-propanol BCC (80 v/v) 500.0 μl of polyethylene glycol 4000 BCC (20 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 275.0 μl of water (100 v/v) |
B7 |
| pH 4 | 40.0 μl of ammonium acetate BCC (5 M) 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 6.0 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 94.0 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 110.0 μl of water (100 v/v) |
B8 |
| pH 5 | 100.0 μl of magnesium chloride BCC (1 M) 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 50.0 μl of water (100 v/v) |
B9 |
| pH 6 | 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 150.0 μl of water (100 v/v) |
B10 |
| pH 6 | 150.0 μl of ammonium sulfate BCC (1 M) 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) |
B11 |
| pH 7 | 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 150.0 μl of water (100 v/v) |
B12 |
| pH 7 | 100.0 μl of magnesium chloride BCC (1 M) 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 50.0 μl of water (100 v/v) |
C1 |
| pH 8 | 150.0 μl of ammonium sulfate BCC (1 M) 750.0 μl of polyethylene glycol 4000 BCC (20 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) |
C2 |
| pH 4.5 | 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 56.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 44.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
C3 |
| pH 5 | 40.0 μl of ammonium acetate BCC (5 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 460.0 μl of water (100 v/v) |
C4 |
| pH 6 | 200.0 μl of lithium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
C5 |
| pH 8 | 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 500.0 μl of water (100 v/v) |
C6 |
| pH 7 | 150.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
C7 |
| pH 5.6 | 250.0 μl of 2-propanol BCC (80 v/v) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250.0 μl of water (100 v/v) |
C8 |
| pH 8 | 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of sodium chloride BCC (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
C9 |
| pH 5.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 94.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 5.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 400.0 μl of water (100 v/v) |
C10 |
| pH 5.5 | 150.0 μl of ammonium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 90.0 μl of sodium MES BCC pH 5.2 (1 M) 10.0 μl of sodium MES BCC pH 7.2 (1 M) 250.0 μl of water (100 v/v) |
C11 |
| pH 6.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 400.0 μl of water (100 v/v) |
C12 |
| pH 6.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 40.0 μl of potassium iodide BCC (5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 360.0 μl of water (100 v/v) |
D1 |
| pH 7.5 | 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of sodium chloride BCC (1 M) 200.0 μl of water (100 v/v) |
D2 |
| pH 6.5 | 150.0 μl of 1-propanol BCC (80 v/v) 500.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 250.0 μl of water (100 v/v) |
D3 |
| pH 7 | 750.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 100.0 μl of potassium chloride BCC (1 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 50.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 200.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
D5 |
| pH 6 | 100.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 6000 BCC (20 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 400.0 μl of water (100 v/v) |
D6 |
| pH 8 | 400.0 μl of polyethylene glycol 6000 BCC (20 w/v) 150.0 μl of sodium chloride BCC (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 350.0 μl of water (100 v/v) |
D7 |
| pH 5.5 | 750.0 μl of polyethylene glycol 6000 BCC (20 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 150.0 μl of water (100 v/v) |
D8 |
| pH 6.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 750.0 μl of polyethylene glycol 6000 BCC (20 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 110.0 μl of water (100 v/v) |
D9 |
| pH 6.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 750.0 μl of polyethylene glycol 6000 BCC (20 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 100.0 μl of water (100 v/v) |
D10 |
| pH 7.5 | 750.0 μl of polyethylene glycol 6000 BCC (20 w/v) 100.0 μl of potassium chloride BCC (1 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 50.0 μl of water (100 v/v) |
D11 |
| pH 7.5 | 750.0 μl of polyethylene glycol 6000 BCC (20 w/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 150.0 μl of water (100 v/v) |
D12 |
| pH 8.5 | 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 500.0 μl of water (100 v/v) |
E1 |
| pH 4.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 8000 BCC (20 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 460.0 μl of water (100 v/v) |
E2 |
| pH 5 | 400.0 μl of polyethylene glycol 8000 BCC (20 w/v) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
E3 |
| pH 6 | 400.0 μl of polyethylene glycol 8000 BCC (20 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 200.0 μl of sodium chloride BCC (1 M) 300.0 μl of water (100 v/v) |
E4 |
| pH 7 | 400.0 μl of polyethylene glycol 8000 BCC (20 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
E5 |
| pH 8 | 400.0 μl of polyethylene glycol 8000 BCC (20 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 500.0 μl of water (100 v/v) |
E6 |
| pH 5.5 | 100.0 μl of calcium acetate BCC (1 M) 600.0 μl of polyethylene glycol 8000 BCC (20 w/v) 94.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 5.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 200.0 μl of water (100 v/v) |
E7 |
| NA | 600.0 μl of polyethylene glycol 8000 BCC (20 w/v) 100.0 μl of sodium dihydrogen phosphate BCC (1 M) 300.0 μl of water (100 v/v) |
E8 |
| pH 7.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 600.0 μl of polyethylene glycol 8000 BCC (20 w/v) 59.4 μl of sodium MOPS BCC pH 6.2 (0.5 M) 140.6 μl of sodium MOPS BCC pH 8.2 (0.5 M) 160.0 μl of water (100 v/v) |
E9 |
| pH 7.5 | 600.0 μl of polyethylene glycol 8000 BCC (20 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of sodium chloride BCC (1 M) 100.0 μl of water (100 v/v) |
E10 |
| pH 8.5 | 200.0 μl of ammonium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 8000 BCC (20 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
E11 |
| pH 5 | 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
E12 |
| pH 6.5 | 200.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
F1 |
| pH 7 | 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
F2 |
| pH 8 | 200.0 μl of lithium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
F3 |
| pH 6.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 333.3 μl of polyethylene glycol 10000 BCC (30 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 526.7 μl of water (100 v/v) |
F4 |
| pH 7 | 600.0 μl of polyethylene glycol 10000 BCC (30 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F5 |
| pH 8 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 100.0 μl of sodium chloride BCC (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 480.0 μl of water (100 v/v) |
F6 |
| pH 7 | 600.0 μl of polyethylene glycol 20000 BCC (25 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F7 |
| pH 6.5 | 500.0 μl of ammonium sulfate BCC (1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 400.0 μl of water (100 v/v) |
F8 |
| pH 5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 614.3 μl of water (100 v/v) |
F9 |
| pH 6.5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 614.3 μl of water (100 v/v) |
F10 |
| pH 8 | 285.7 μl of ammonium sulfate BCC (3.5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 614.3 μl of water (100 v/v) |
F11 |
| pH 5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 471.4 μl of water (100 v/v) |
F12 |
| pH 7 | 428.6 μl of ammonium sulfate BCC (3.5 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 471.4 μl of water (100 v/v) |
G1 |
| pH 8 | 428.6 μl of ammonium sulfate BCC (3.5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 471.4 μl of water (100 v/v) |
G2 |
| pH 5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 328.6 μl of water (100 v/v) |
G3 |
| pH 7 | 571.4 μl of ammonium sulfate BCC (3.5 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v) |
G4 |
| pH 8 | 571.4 μl of ammonium sulfate BCC (3.5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 328.6 μl of water (100 v/v) |
G5 |
| pH 7 | 285.7 μl of ammonium sulfate BCC (3.5 M) 333.3 μl of potassium chloride BCC (3 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 281.0 μl of water (100 v/v) |
G6 |
| pH 5 | 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium formate BCC (4 M) 400.0 μl of water (100 v/v) |
G7 |
| pH 7.5 | 750.0 μl of sodium formate BCC (4 M) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 150.0 μl of water (100 v/v) |
G8 |
| pH 7.5 | 42.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 224.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 733.3 μl of water (100 v/v) |
G9 |
| pH 7 | 156.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 277.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 566.7 μl of water (100 v/v) |
G10 |
| pH 6.5 | 352.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 181.3 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 466.7 μl of water (100 v/v) |
G11 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 333.3 μl of sodium acetate BCC (3 M) 566.7 μl of water (100 v/v) |
G12 |
| pH 7 | 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 275.0 μl of water (100 v/v) |
H1 |
| pH 6 | 400.0 μl of sodium chloride BCC (5 M) 90.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
H2 |
| pH 6.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 500.0 μl of water (100 v/v) |
H3 |
| pH 8 | 640.0 μl of lithium sulfate BCC (2.5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 260.0 μl of water (100 v/v) |
H4 |
| pH 6 | 411.8 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M) 588.2 μl of water (100 v/v) |
H5 |
| pH 8 | 600.0 μl of potassium sodium tartrate BCC (2 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
H6 |
| pH 6.5 | 640.0 μl of magnesium sulfate BCC (2.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 260.0 μl of water (100 v/v) |
H7 |
| pH 5 | 150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of polyethylene glycol 4000 BCC (20 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 650.0 μl of water (100 v/v) |
H8 |
| pH 6 | 250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of calcium acetate BCC (1 M) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 600.0 μl of water (100 v/v) |
H9 |
| pH 7 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 52.4 μl of imidazole BCC pH 6 (1 M) 47.6 μl of imidazole BCC pH 8 (1 M) 400.0 μl of water (100 v/v) |
H10 |
| pH 6.5 | 125.0 μl of 2-propanol BCC (80 v/v) 50.0 μl of magnesium chloride BCC (1 M) 250.0 μl of polyethylene glycol 4000 BCC (20 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 475.0 μl of water (100 v/v) |
H11 |
| pH 7.5 | 312.5 μl of 2-propanol BCC (80 v/v) 40.0 μl of ammonium acetate BCC (5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 547.5 μl of water (100 v/v) |
H12 |
| pH 8 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 150.0 μl of ethanol BCC (100 v/v) 100.0 μl of sodium chloride BCC (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 600.0 μl of water (100 v/v) |