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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
PI_PI2_C3
BCC Core Screens / PI_PI2_C3
Created 2025-03-06.
1-48 is PEG/Ion, 49-96 is PEG/Ion II. PEG/Ion 2 reagent 43 used to be 20% PEG 3350, 0.02M NiCl2, 0.02M MgCl2, 0.02M FeCl3 - but the ferric chloride made the condition very acidic, and Hampton Research dropped this formulation starting from Lot 209802
Export
PI_PI2_C3
Barcodes
PI_PI2_C3
Screen Structure
PI_PI2_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 67 |
Internal diversity (score mean dist) | 0.689 |
Screen Composition
ingredient type | PI_PI2_C3 |
---|---|
Buffer | 47 |
Complex ingredient | 15 |
Polymer | 96 |
Salt | 88 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium fluoride BCC (1 M) 400.0 μl of water (100 v/v) |
A2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium fluoride BCC (1 M) 400.0 μl of water (100 v/v) |
A3 |
| NA | 200.0 μl of ammonium fluoride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A4 |
| NA | 200.0 μl of lithium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A5 |
| NA | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A6 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium chloride BCC (1 M) 400.0 μl of water (100 v/v) |
A7 |
| NA | 200.0 μl of calcium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A8 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium chloride BCC (1 M) 400.0 μl of water (100 v/v) |
A9 |
| NA | 200.0 μl of ammonium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium iodide BCC (1 M) 400.0 μl of water (100 v/v) |
A11 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of potassium iodide BCC (5 M) 560.0 μl of water (100 v/v) |
A12 |
| NA | 200.0 μl of ammonium iodide BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B1 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
B2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
B3 |
| NA | 200.0 μl of lithium nitrate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B4 |
| NA | 200.0 μl of magnesium nitrate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B5 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium nitrate BCC (1 M) 400.0 μl of water (100 v/v) |
B6 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium nitrate BCC (1 M) 400.0 μl of water (100 v/v) |
B7 |
| NA | 200.0 μl of ammonium nitrate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B8 |
| NA | 200.0 μl of magnesium formate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B9 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium formate BCC (4 M) 550.0 μl of water (100 v/v) |
B10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium formate BCC (1 M) 400.0 μl of water (100 v/v) |
B11 |
| NA | 200.0 μl of ammonium formate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
B12 |
| NA | 80.0 μl of lithium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 520.0 μl of water (100 v/v) |
C1 |
| NA | 80.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 520.0 μl of water (100 v/v) |
C2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of zinc acetate BCC (1 M) 400.0 μl of water (100 v/v) |
C3 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 400.0 μl of water (100 v/v) |
C4 |
| NA | 200.0 μl of calcium acetate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
C5 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of potassium acetate BCC (5 M) 560.0 μl of water (100 v/v) |
C6 |
| NA | 40.0 μl of ammonium acetate BCC (5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 560.0 μl of water (100 v/v) |
C7 |
| NA | 200.0 μl of lithium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
C8 |
| NA | 200.0 μl of magnesium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
C9 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 166.7 μl of sodium sulfate BCC (1.2 M) 433.3 μl of water (100 v/v) |
C10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of potassium sulfate BCC (0.5 M) 200.0 μl of water (100 v/v) |
C11 |
| NA | 200.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
C12 |
| NA | 200.0 μl of disodium tartrate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
D1 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium sodium tartrate BCC (2 M) 500.0 μl of water (100 v/v) |
D2 |
| NA | 100.0 μl of diammonium tartrate BCC (2 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
D3 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium dihydrogen phosphate BCC (1 M) 400.0 μl of water (100 v/v) |
D4 |
| pH 9.3 | 400.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
D5 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of potassium dihydrogen phosphate BCC (1 M) 400.0 μl of water (100 v/v) |
D6 |
| NA | 100.0 μl of dipotassium hydrogen phosphate BCC (2 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
D7 |
| NA | 80.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 520.0 μl of water (100 v/v) |
D8 |
| NA | 57.1 μl of diammonium hydrogen phosphate BCC (3.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 542.9 μl of water (100 v/v) |
D9 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of trilithium citrate BCC (1 M) 400.0 μl of water (100 v/v) |
D10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 125.0 μl of trisodium citrate BCC (1.6 M) 475.0 μl of water (100 v/v) |
D11 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of tripotassium citrate BCC (1 M) 400.0 μl of water (100 v/v) |
D12 |
| NA | 100.0 μl of diammonium hydrogen citrate BCC (2 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
E1 |
| pH 4 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 29.4 μl of sodium malonate-malonic acid BCC pH 4 (3.4 M) 730.6 μl of water (100 v/v) |
E2 |
| pH 4 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 4 (3.4 M) 541.2 μl of water (100 v/v) |
E3 |
| pH 5 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 29.4 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M) 730.6 μl of water (100 v/v) |
E4 |
| pH 5 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M) 541.2 μl of water (100 v/v) |
E5 |
| pH 6 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 29.4 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M) 730.6 μl of water (100 v/v) |
E6 |
| pH 6 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M) 541.2 μl of water (100 v/v) |
E7 |
| pH 7 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 29.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 730.6 μl of water (100 v/v) |
E8 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 541.2 μl of water (100 v/v) |
E9 |
| pH 4 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of tacsimate BCC pH 4 (100 v/v) 720.0 μl of water (100 v/v) |
E10 |
| pH 4 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of tacsimate BCC pH 4 (100 v/v) 520.0 μl of water (100 v/v) |
E11 |
| pH 5 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of tacsimate BCC pH 5 (100 v/v) 720.0 μl of water (100 v/v) |
E12 |
| pH 5 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of tacsimate BCC pH 5 (100 v/v) 520.0 μl of water (100 v/v) |
F1 |
| pH 6 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of tacsimate BCC pH 6 (100 v/v) 720.0 μl of water (100 v/v) |
F2 |
| pH 6 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of tacsimate BCC pH 6 (100 v/v) 520.0 μl of water (100 v/v) |
F3 |
| pH 7 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of tacsimate BCC pH 7 (100 v/v) 720.0 μl of water (100 v/v) |
F4 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of tacsimate BCC pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
F5 |
| pH 8 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of tacsimate BCC pH 8 (100 v/v) 720.0 μl of water (100 v/v) |
F6 |
| pH 8 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of tacsimate BCC pH 8 (100 v/v) 520.0 μl of water (100 v/v) |
F7 |
| pH 7 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium succinate BCC pH 7 (1 M) 660.0 μl of water (100 v/v) |
F8 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium succinate BCC pH 7 (1 M) 400.0 μl of water (100 v/v) |
F9 |
| NA | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of triammonium citrate BCC (2.5 M) 720.0 μl of water (100 v/v) |
F10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 80.0 μl of triammonium citrate BCC (2.5 M) 520.0 μl of water (100 v/v) |
F11 |
| pH 7 | 100.0 μl of DL-malic acid BCC pH 7 (1 M) 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 660.0 μl of water (100 v/v) |
F12 |
| pH 7 | 200.0 μl of DL-malic acid BCC pH 7 (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
G1 |
| NA | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium acetate BCC (1 M) 660.0 μl of water (100 v/v) |
G2 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 400.0 μl of water (100 v/v) |
G3 |
| NA | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 25.0 μl of sodium formate BCC (4 M) 735.0 μl of water (100 v/v) |
G4 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium formate BCC (4 M) 550.0 μl of water (100 v/v) |
G5 |
| NA | 50.0 μl of diammonium tartrate BCC (2 M) 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 710.0 μl of water (100 v/v) |
G6 |
| NA | 100.0 μl of diammonium tartrate BCC (2 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
G7 |
| NA | 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 20.0 μl of tacsimate BCC pH 4 (100 v/v) 560.0 μl of water (100 v/v) |
G8 |
| NA | 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of tacsimate BCC pH 5 (100 v/v) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 560.0 μl of water (100 v/v) |
G9 |
| NA | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of tacsimate BCC pH 6 (100 v/v) 480.0 μl of water (100 v/v) |
G10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 20.0 μl of tacsimate BCC pH 7 (100 v/v) 480.0 μl of water (100 v/v) |
G11 |
| NA | 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of tacsimate BCC pH 8 (100 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 560.0 μl of water (100 v/v) |
G12 |
| pH 3.4 | 32.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 68.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 580.0 μl of water (100 v/v) |
H1 |
| pH 4.1 | 42.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 58.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 580.0 μl of water (100 v/v) |
H2 |
| pH 5 | 52.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 48.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 580.0 μl of water (100 v/v) |
H3 |
| pH 6.4 | 62.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 38.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
H4 |
| pH 7.6 | 72.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 28.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
H5 |
| pH 8.8 | 82.0 μl of citrate-bis-tris propane BCC pH 10 (1 M) 18.0 μl of citrate-bis-tris propane BCC pH 2.3 (1 M) 320.0 μl of polyethylene glycol 3350 BCC (50 w/v) 580.0 μl of water (100 v/v) |
H6 |
| NA | 200.0 μl of cadmium chloride BCC (0.1 M) 20.0 μl of calcium chloride BCC (1 M) 200.0 μl of cobalt chloride BCC (0.1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 180.0 μl of water (100 v/v) |
H7 |
| pH 7 | 10.0 μl of magnesium chloride BCC (1 M) 5.0 μl of nickel(II) chloride BCC (1 M) 300.0 μl of polyethylene glycol 3350 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 585.0 μl of water (100 v/v) |
H8 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of zinc chloride BCC (1 M) 580.0 μl of water (100 v/v) |
H9 |
| NA | 150.0 μl of cesium chloride BCC (1 M) 300.0 μl of polyethylene glycol 3350 BCC (50 w/v) 550.0 μl of water (100 v/v) |
H10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of sodium bromide BCC (1 M) 400.0 μl of water (100 v/v) |
H11 |
| pH 7 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.9 μl of sodium HEPES BCC pH 6.5 (1 M) 9.1 μl of sodium HEPES BCC pH 8.5 (1 M) 100.0 μl of tryptone BCC (10 w/v) 610.0 μl of water (100 v/v) |
H12 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.9 μl of sodium HEPES BCC pH 6.5 (1 M) 9.1 μl of sodium HEPES BCC pH 8.5 (1 M) 100.0 μl of tryptone BCC (10 w/v) 450.0 μl of water (100 v/v) |