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Supplemental Information

Screen Description

PI_PI2_C3

BCC Core Screens / PI_PI2_C3
Created 2025-03-06.
1-48 is PEG/Ion, 49-96 is PEG/Ion II. PEG/Ion 2 reagent 43 used to be 20% PEG 3350, 0.02M NiCl2, 0.02M MgCl2, 0.02M FeCl3 - but the ferric chloride made the condition very acidic, and Hampton Research dropped this formulation starting from Lot 209802

Export

PI_PI2_C3

Barcodes

PI_PI2_C3

  • Z0002747
  • Z0002748

Screen Structure

PI_PI2_C3

Well Count 96
Number of distinct ingredients 67
Internal diversity (score mean dist) 0.689

Screen Composition

More information about screen composition chart.
2025-06-12T22:59:02.841313 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type PI_PI2_C3
Buffer 47
Complex ingredient 15
Polymer 96
Salt 88

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PI_PI2_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium fluoride BCC 0.2 M -
NA
200 μl of sodium fluoride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A2
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium fluoride BCC 0.2 M -
NA
200 μl of potassium fluoride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A3
  • ammonium fluoride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium fluoride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A4
  • lithium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of lithium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A5
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of magnesium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A6
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
NA
200 μl of sodium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A7
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of calcium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A8
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium chloride BCC 0.2 M -
NA
200 μl of potassium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A9
  • ammonium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A10
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium iodide BCC 0.2 M -
NA
200 μl of sodium iodide BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
A11
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium iodide BCC 0.2 M -
NA
40 μl of potassium iodide BCC (5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
A12
  • ammonium iodide BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium iodide BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B1
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium thiocyanate BCC 0.2 M -
NA
100 μl of sodium thiocyanate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
B2
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium thiocyanate BCC 0.2 M -
NA
100 μl of potassium thiocyanate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
B3
  • lithium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of lithium nitrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B4
  • magnesium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of magnesium nitrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B5
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium nitrate BCC 0.2 M -
NA
200 μl of sodium nitrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B6
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium nitrate BCC 0.2 M -
NA
200 μl of potassium nitrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B7
  • ammonium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium nitrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B8
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of magnesium formate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B9
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium formate BCC 0.2 M -
NA
50 μl of sodium formate BCC (4 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
550 μl of water (100 v/v)
B10
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium formate BCC 0.2 M -
NA
200 μl of potassium formate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B11
  • polyethylene glycol 3350 BCC 20 w/v -
  • ammonium formate BCC 0.2 M -
NA
200 μl of ammonium formate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
B12
  • lithium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80 μl of lithium acetate BCC (2.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
C1
  • magnesium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80 μl of magnesium acetate BCC (2.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
C2
  • polyethylene glycol 3350 BCC 20 w/v -
  • zinc acetate BCC 0.2 M -
NA
200 μl of zinc acetate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C3
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium acetate BCC 0.2 M -
NA
200 μl of sodium acetate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C4
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of calcium acetate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C5
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium acetate BCC 0.2 M -
NA
40 μl of potassium acetate BCC (5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
C6
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
40 μl of ammonium acetate BCC (5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
C7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of lithium sulfate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C8
  • polyethylene glycol 3350 BCC 20 w/v -
  • magnesium sulfate BCC 0.2 M -
NA
200 μl of magnesium sulfate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C9
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium sulfate BCC 0.2 M -
NA
166 μl of sodium sulfate BCC (1.2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
433 μl of water (100 v/v)
C10
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium sulfate BCC 0.2 M -
NA
400 μl of potassium sulfate BCC (0.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200 μl of ammonium sulfate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
C12
  • polyethylene glycol 3350 BCC 20 w/v -
  • disodium tartrate BCC 0.2 M -
NA
200 μl of disodium tartrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D1
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium sodium tartrate BCC 0.2 M -
NA
100 μl of potassium sodium tartrate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
D2
  • polyethylene glycol 3350 BCC 20 w/v -
  • diammonium tartrate BCC 0.2 M -
NA
100 μl of diammonium tartrate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
D3
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium dihydrogen phosphate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of sodium dihydrogen phosphate BCC (1 M)
400 μl of water (100 v/v)
D4
  • disodium hydrogen phosphate BCC 0.2 M pH 9.3
  • polyethylene glycol 3350 BCC 20 w/v -
pH 9.3
400 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
D5
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium dihydrogen phosphate BCC 0.2 M -
NA
200 μl of potassium dihydrogen phosphate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D6
  • dipotassium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
100 μl of dipotassium hydrogen phosphate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
D7
  • ammonium dihydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
D8
  • diammonium hydrogen phosphate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
57 μl of diammonium hydrogen phosphate BCC (3.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
542 μl of water (100 v/v)
D9
  • polyethylene glycol 3350 BCC 20 w/v -
  • trilithium citrate BCC 0.2 M -
NA
200 μl of trilithium citrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D10
  • polyethylene glycol 3350 BCC 20 w/v -
  • trisodium citrate BCC 0.2 M -
NA
125 μl of trisodium citrate BCC (1.6 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
475 μl of water (100 v/v)
D11
  • polyethylene glycol 3350 BCC 20 w/v -
  • tripotassium citrate BCC 0.2 M -
NA
200 μl of tripotassium citrate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D12
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
100 μl of diammonium hydrogen citrate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
E1
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate-malonic acid BCC 0.1 M pH 4
pH 4
29 μl of sodium malonate-malonic acid BCC pH 4 (3.4 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
730 μl of water (100 v/v)
E2
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 4
pH 4
58 μl of sodium malonate-malonic acid BCC pH 4 (3.4 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
541 μl of water (100 v/v)
E3
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate-malonic acid BCC 0.1 M pH 5
pH 5
29 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
730 μl of water (100 v/v)
E4
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 5
pH 5
58 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
541 μl of water (100 v/v)
E5
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate-malonic acid BCC 0.1 M pH 6
pH 6
240 μl of polyethylene glycol 3350 BCC (50 w/v)
29 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
730 μl of water (100 v/v)
E6
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 6
pH 6
400 μl of polyethylene glycol 3350 BCC (50 w/v)
58 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
541 μl of water (100 v/v)
E7
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate-malonic acid BCC 0.1 M pH 7
pH 7
29 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
730 μl of water (100 v/v)
E8
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 7
pH 7
58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
541 μl of water (100 v/v)
E9
  • polyethylene glycol 3350 BCC 12 w/v -
  • tacsimate BCC 4 v/v pH 4
pH 4
40 μl of tacsimate BCC pH 4 (100 v/v)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
720 μl of water (100 v/v)
E10
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 8 v/v pH 4
pH 4
80 μl of tacsimate BCC pH 4 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
E11
  • polyethylene glycol 3350 BCC 12 w/v -
  • tacsimate BCC 4 v/v pH 5
pH 5
40 μl of tacsimate BCC pH 5 (100 v/v)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
720 μl of water (100 v/v)
E12
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 8 v/v pH 5
pH 5
80 μl of tacsimate BCC pH 5 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
F1
  • polyethylene glycol 3350 BCC 12 w/v -
  • tacsimate BCC 4 v/v pH 6
pH 6
40 μl of tacsimate BCC pH 6 (100 v/v)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
720 μl of water (100 v/v)
F2
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 8 v/v pH 6
pH 6
80 μl of tacsimate BCC pH 6 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
F3
  • polyethylene glycol 3350 BCC 12 w/v -
  • tacsimate BCC 4 v/v pH 7
pH 7
40 μl of tacsimate BCC pH 7 (100 v/v)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
720 μl of water (100 v/v)
F4
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 8 v/v pH 7
pH 7
80 μl of tacsimate BCC pH 7 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
F5
  • polyethylene glycol 3350 BCC 12 w/v -
  • tacsimate BCC 4 v/v pH 8
pH 8
40 μl of tacsimate BCC pH 8 (100 v/v)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
720 μl of water (100 v/v)
F6
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 8 v/v pH 8
pH 8
80 μl of tacsimate BCC pH 8 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
F7
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium succinate BCC 0.1 M pH 7
pH 7
100 μl of sodium succinate BCC pH 7 (1 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
660 μl of water (100 v/v)
F8
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium succinate BCC 0.2 M pH 7
pH 7
200 μl of sodium succinate BCC pH 7 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
F9
  • polyethylene glycol 3350 BCC 12 w/v -
  • triammonium citrate BCC 0.1 M -
NA
240 μl of polyethylene glycol 3350 BCC (50 w/v)
40 μl of triammonium citrate BCC (2.5 M)
720 μl of water (100 v/v)
F10
  • polyethylene glycol 3350 BCC 20 w/v -
  • triammonium citrate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
80 μl of triammonium citrate BCC (2.5 M)
520 μl of water (100 v/v)
F11
  • polyethylene glycol 3350 BCC 12 w/v -
  • DL-malic acid BCC 0.1 M pH 7
pH 7
100 μl of DL-malic acid BCC pH 7 (1 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
660 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 20 w/v -
  • DL-malic acid BCC 0.2 M pH 7
pH 7
200 μl of DL-malic acid BCC pH 7 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
G1
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium acetate BCC 0.1 M -
NA
100 μl of sodium acetate BCC (1 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
660 μl of water (100 v/v)
G2
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium acetate BCC 0.2 M -
NA
200 μl of sodium acetate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
G3
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium formate BCC 0.1 M -
NA
25 μl of sodium formate BCC (4 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
735 μl of water (100 v/v)
G4
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium formate BCC 0.2 M -
NA
50 μl of sodium formate BCC (4 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
550 μl of water (100 v/v)
G5
  • polyethylene glycol 3350 BCC 12 w/v -
  • diammonium tartrate BCC 0.1 M -
NA
50 μl of diammonium tartrate BCC (2 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
710 μl of water (100 v/v)
G6
  • polyethylene glycol 3350 BCC 20 w/v -
  • diammonium tartrate BCC 0.2 M -
NA
100 μl of diammonium tartrate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
G7
  • polyethylene glycol 3350 BCC 16 w/v -
  • tacsimate BCC 2 v/v pH 4
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
NA
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
20 μl of tacsimate BCC pH 4 (100 v/v)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
G8
  • polyethylene glycol 3350 BCC 16 w/v -
  • tacsimate BCC 2 v/v pH 5
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
NA
18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
20 μl of tacsimate BCC pH 5 (100 v/v)
82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
G9
  • polyethylene glycol 3350 BCC 20 w/v -
  • tacsimate BCC 2 v/v pH 6
  • bis-tris chloride BCC 0.1 M pH 6.5
NA
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
20 μl of tacsimate BCC pH 6 (100 v/v)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
480 μl of water (100 v/v)
G10
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • tacsimate BCC 2 v/v pH 7
NA
20 μl of tacsimate BCC pH 7 (100 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
480 μl of water (100 v/v)
G11
  • polyethylene glycol 3350 BCC 16 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • tacsimate BCC 2 v/v pH 8
NA
78 μl of tris chloride BCC pH 9 (1 M)
20 μl of tacsimate BCC pH 8 (100 v/v)
21 μl of tris chloride BCC pH 7 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
560 μl of water (100 v/v)
G12
  • polyethylene glycol 3350 BCC 16 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 3.4
pH 3.4
32 μl of citrate-bis-tris propane BCC pH 10 (1 M)
68 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
580 μl of water (100 v/v)
H1
  • polyethylene glycol 3350 BCC 16 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 4.1
pH 4.1
42 μl of citrate-bis-tris propane BCC pH 10 (1 M)
58 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
580 μl of water (100 v/v)
H2
  • polyethylene glycol 3350 BCC 16 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 5
pH 5
52 μl of citrate-bis-tris propane BCC pH 10 (1 M)
48 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
580 μl of water (100 v/v)
H3
  • polyethylene glycol 3350 BCC 20 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 6.4
pH 6.4
62 μl of citrate-bis-tris propane BCC pH 10 (1 M)
38 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
H4
  • polyethylene glycol 3350 BCC 20 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 7.6
pH 7.6
72 μl of citrate-bis-tris propane BCC pH 10 (1 M)
28 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
H5
  • polyethylene glycol 3350 BCC 16 w/v -
  • citrate-bis-tris propane BCC 0.1 M pH 8.8
pH 8.8
82 μl of citrate-bis-tris propane BCC pH 10 (1 M)
18 μl of citrate-bis-tris propane BCC pH 2.3 (1 M)
320 μl of polyethylene glycol 3350 BCC (50 w/v)
580 μl of water (100 v/v)
H6
  • cadmium chloride BCC 0.02 M -
  • calcium chloride BCC 0.02 M -
  • cobalt chloride BCC 0.02 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
20 μl of calcium chloride BCC (1 M)
200 μl of cobalt chloride BCC (0.1 M)
200 μl of cadmium chloride BCC (0.1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
180 μl of water (100 v/v)
H7
  • magnesium chloride BCC 0.01 M -
  • nickel(II) chloride BCC 0.005 M -
  • polyethylene glycol 3350 BCC 15 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
10 μl of magnesium chloride BCC (1 M)
5 μl of nickel(II) chloride BCC (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of polyethylene glycol 3350 BCC (50 w/v)
585 μl of water (100 v/v)
H8
  • polyethylene glycol 3350 BCC 20 w/v -
  • zinc chloride BCC 0.02 M -
NA
20 μl of zinc chloride BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
580 μl of water (100 v/v)
H9
  • polyethylene glycol 3350 BCC 15 w/v -
  • cesium chloride BCC 0.15 M -
NA
150 μl of cesium chloride BCC (1 M)
300 μl of polyethylene glycol 3350 BCC (50 w/v)
550 μl of water (100 v/v)
H10
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium bromide BCC 0.2 M -
NA
200 μl of sodium bromide BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
H11
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium HEPES BCC 0.05 M pH 7
  • tryptone BCC 1 w/v -
pH 7
100 μl of tryptone BCC (10 w/v)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
9 μl of sodium HEPES BCC pH 8.5 (1 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
610 μl of water (100 v/v)
H12
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium HEPES BCC 0.05 M pH 7
  • tryptone BCC 1 w/v -
pH 7
100 μl of tryptone BCC (10 w/v)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
9 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
450 μl of water (100 v/v)