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Supplemental Information

Screen Description

MemGold2_C3

BCC Core Screens / MemGold2_C3
Created 2025-03-06.
Based on Molecular Dimensions MemGold2 screen, made up by C3. Tweaked to be only 80 stocks.

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Barcodes

MemGold2_C3

  • Z0002499
  • Z0002768
  • Z0002769
  • Z0002770

Screen Structure

MemGold2_C3

Well Count 96
Number of distinct ingredients 62
Internal diversity (score mean dist) 0.942

Screen Composition

2025-06-12T23:12:11.805819 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type MemGold2_C3
Buffer 92
Organic (non-volatile) 3
Polymer 88
Salt 113

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemGold2_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • cadmium chloride BCC 0.005 M -
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol monomethyl ether 550 BCC 14 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
164 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
80 μl of magnesium chloride BCC (2.5 M)
5 μl of cadmium chloride BCC (1 M)
650 μl of water (100 v/v)
A2
  • polyethylene glycol 3000 BCC 44 w/v -
  • potassium acetate BCC 0.1 M -
  • potassium chloride BCC 0.01 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
10 μl of potassium chloride BCC (1 M)
880 μl of polyethylene glycol 3000 BCC (50 w/v)
20 μl of tris chloride BCC pH 7 (1 M)
20 μl of potassium acetate BCC (5 M)
70 μl of water (100 v/v)
A3
  • polyethylene glycol 1500 BCC 10 w/v -
  • sodium chloride BCC 0.02 M -
  • sodium MES BCC 0.02 M pH 6
  • magnesium sulfate BCC 0.08 M -
pH 6
12 μl of sodium MES BCC pH 5.2 (1 M)
7 μl of sodium MES BCC pH 7.2 (1 M)
80 μl of magnesium sulfate BCC (1 M)
200 μl of polyethylene glycol 1500 BCC (50 w/v)
20 μl of sodium chloride BCC (1 M)
680 μl of water (100 v/v)
A4
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 1500 BCC 8 w/v -
  • sodium MES BCC 0.02 M pH 6.5
  • magnesium sulfate BCC 0.04 M -
pH 6.5
5 μl of sodium MES BCC pH 5.2 (1 M)
14 μl of sodium MES BCC pH 7.2 (1 M)
40 μl of magnesium sulfate BCC (1 M)
20 μl of calcium chloride BCC (1 M)
160 μl of polyethylene glycol 1500 BCC (50 w/v)
760 μl of water (100 v/v)
A5
  • lithium chloride BCC 0.05 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • tris chloride BCC 0.05 M pH 8.5
  • nickel(II) sulfate BCC 0.05 M -
pH 8.5
50 μl of nickel(II) sulfate BCC (1 M)
400 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of lithium chloride BCC (1 M)
10 μl of tris chloride BCC pH 7 (1 M)
39 μl of tris chloride BCC pH 9 (1 M)
450 μl of water (100 v/v)
A6
  • dipotassium hydrogen phosphate BCC 0.1 M pH 9.2
  • polyethylene glycol 3350 BCC 10 w/v -
  • potassium dihydrogen phosphate BCC 0.1 M pH 4.6
  • bis-tris propane chloride BCC 0.1 M pH 7.5
NA
100 μl of potassium dihydrogen phosphate BCC pH 4.6 (1 M)
100 μl of dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
200 μl of polyethylene glycol 3350 BCC (50 w/v)
62 μl of bis-tris propane chloride BCC pH 6 (1 M)
38 μl of bis-tris propane chloride BCC pH 10 (1 M)
500 μl of water (100 v/v)
A7
  • ADA BCC 0.1 M pH 6.5
  • lithium sulfate BCC 0.1 M -
  • polyethylene glycol 4000 BCC 11.5 w/v -
  • sodium chloride BCC 0.1 M -
pH 6.5
109 μl of ADA BCC pH 5.5 (0.5 M)
90 μl of ADA BCC pH 7.5 (0.5 M)
100 μl of sodium chloride BCC (1 M)
100 μl of lithium sulfate BCC (1 M)
230 μl of polyethylene glycol 4000 BCC (50 w/v)
370 μl of water (100 v/v)
A8
  • cadmium chloride BCC 0.1 M -
  • lithium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
375 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of lithium chloride BCC (1 M)
100 μl of cadmium chloride BCC (1 M)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
325 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 0.2 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
  • polyethylene glycol 2000 BCC 20 w/v -
pH 6
57 μl of ammonium sulfate BCC (3.5 M)
400 μl of polyethylene glycol 2000 BCC (50 w/v)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
100 μl of sodium chloride BCC (1 M)
342 μl of water (100 v/v)
A10
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 31 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
387 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of sodium chloride BCC (1 M)
200 μl of lithium sulfate BCC (1 M)
212 μl of water (100 v/v)
A11
  • ammonium dihydrogen phosphate BCC 0.2 M -
  • ammonium sulfate BCC 0.1 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
28 μl of ammonium sulfate BCC (3.5 M)
44 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
56 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
80 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400 μl of polyethylene glycol 400 BCC (80 v/v)
391 μl of water (100 v/v)
A12
  • polyethylene glycol 4000 BCC 14 w/v -
  • potassium chloride BCC 0.12 M -
  • trisodium citrate BCC 0.05 M pH 8.1
  • bis-tris chloride BCC 0.08 M pH 6
NA
120 μl of potassium chloride BCC (1 M)
50 μl of trisodium citrate BCC pH 8.1 (1 M)
50 μl of bis-tris chloride BCC pH 5.5 (1 M)
29 μl of bis-tris chloride BCC pH 6.5 (1 M)
280 μl of polyethylene glycol 4000 BCC (50 w/v)
470 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 1000 BCC 19 w/v -
  • sodium chloride BCC 0.1 M -
  • sodium MES BCC 0.01 M pH 6.5
pH 6.5
42 μl of ammonium sulfate BCC (3.5 M)
2 μl of sodium MES BCC pH 5.2 (1 M)
7 μl of sodium MES BCC pH 7.2 (1 M)
380 μl of polyethylene glycol 1000 BCC (50 w/v)
100 μl of sodium chloride BCC (1 M)
467 μl of water (100 v/v)
B2
  • trisodium citrate-citric acid BCC 0.1 M pH 6
  • polyethylene glycol monomethyl ether 2000 BCC 18 w/v -
  • nickel(II) sulfate BCC 0.01 M -
pH 6
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
10 μl of nickel(II) sulfate BCC (1 M)
360 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
530 μl of water (100 v/v)
B3
  • magnesium chloride BCC 0.02 M -
  • polyethylene glycol 3350 BCC 3.5 w/v -
  • sodium MES BCC 0.02 M pH 6
pH 6
12 μl of sodium MES BCC pH 5.2 (1 M)
7 μl of sodium MES BCC pH 7.2 (1 M)
70 μl of polyethylene glycol 3350 BCC (50 w/v)
8 μl of magnesium chloride BCC (2.5 M)
902 μl of water (100 v/v)
B4
  • sodium chloride BCC 0.02 M -
  • sodium MES BCC 0.05 M pH 5.5
  • polyethylene glycol monomethyl ether 350 BCC 14 v/v -
pH 5.5
45 μl of sodium MES BCC pH 5.2 (1 M)
5 μl of sodium MES BCC pH 7.2 (1 M)
175 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
20 μl of sodium chloride BCC (1 M)
755 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.025 M -
  • polyethylene glycol monomethyl ether 550 BCC 35 v/v -
  • sodium MOPS BCC 0.02 M pH 7
pH 7
25 μl of sodium MOPS BCC pH 6.2 (0.5 M)
14 μl of sodium MOPS BCC pH 8.2 (0.5 M)
411 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
10 μl of magnesium chloride BCC (2.5 M)
538 μl of water (100 v/v)
B6
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
350 μl of polyethylene glycol 400 BCC (80 v/v)
12 μl of magnesium chloride BCC (2.5 M)
538 μl of water (100 v/v)
B7
  • sodium chloride BCC 0.04 M -
  • tris chloride BCC 0.04 M pH 8
  • polyethylene glycol monomethyl ether 350 BCC 25 v/v -
pH 8
21 μl of tris chloride BCC pH 7 (1 M)
312 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
18 μl of tris chloride BCC pH 9 (1 M)
40 μl of sodium chloride BCC (1 M)
607 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 36 v/v -
  • magnesium acetate BCC 0.04 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
360 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
16 μl of magnesium acetate BCC (2.5 M)
524 μl of water (100 v/v)
B9
  • ADA BCC 0.05 M pH 6.3
  • zinc acetate BCC 0.05 M -
  • polyethylene glycol 8000 BCC 11 w/v -
pH 6.3
50 μl of zinc acetate BCC (1 M)
220 μl of polyethylene glycol 8000 BCC (50 w/v)
68 μl of ADA BCC pH 5.5 (0.5 M)
32 μl of ADA BCC pH 7.5 (0.5 M)
630 μl of water (100 v/v)
B10
  • magnesium acetate BCC 0.05 M -
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
325 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of magnesium acetate BCC (2.5 M)
555 μl of water (100 v/v)
B11
  • magnesium acetate BCC 0.05 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • glycine BCC 0.1 M pH 9.5
pH 9.5
400 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of magnesium acetate BCC (2.5 M)
9 μl of glycine BCC pH 8.5 (1 M)
90 μl of glycine BCC pH 10 (1 M)
480 μl of water (100 v/v)
B12
  • polyethylene glycol 4000 BCC 3 w/v -
  • sodium chloride BCC 0.066 M -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
16 μl of tris chloride BCC pH 7 (1 M)
3 μl of tris chloride BCC pH 9 (1 M)
66 μl of sodium chloride BCC (1 M)
60 μl of polyethylene glycol 4000 BCC (50 w/v)
854 μl of water (100 v/v)
C1
  • magnesium chloride BCC 0.075 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 6.5
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
30 μl of magnesium chloride BCC (2.5 M)
270 μl of water (100 v/v)
C2
  • magnesium acetate BCC 0.08 M -
  • polyethylene glycol monomethyl ether 5000 BCC 14 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32 μl of magnesium acetate BCC (2.5 M)
700 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
168 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 2 M -
  • sodium MES BCC 0.1 M pH 6
  • zinc acetate BCC 0.05 M -
pH 6
571 μl of ammonium sulfate BCC (3.5 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
50 μl of zinc acetate BCC (1 M)
278 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 0.087 M -
  • tris chloride BCC 0.05 M pH 7
  • polyethylene glycol dimethyl ether 250 BCC 22 v/v -
pH 7
24 μl of ammonium sulfate BCC (3.5 M)
50 μl of tris chloride BCC pH 7 (1 M)
275 μl of polyethylene glycol dimethyl ether 250 BCC (80 v/v)
650 μl of water (100 v/v)
C5
  • magnesium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 13 w/v -
pH 7.5
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
260 μl of polyethylene glycol 8000 BCC (50 w/v)
40 μl of magnesium chloride BCC (2.5 M)
600 μl of water (100 v/v)
C6
  • magnesium formate BCC 0.1 M -
  • polyethylene glycol 3350 BCC 17 w/v -
  • sodium MOPS BCC 0.1 M pH 7
pH 7
340 μl of polyethylene glycol 3350 BCC (50 w/v)
127 μl of sodium MOPS BCC pH 6.2 (0.5 M)
72 μl of sodium MOPS BCC pH 8.2 (0.5 M)
100 μl of magnesium formate BCC (1 M)
360 μl of water (100 v/v)
C7
  • polyethylene glycol 4000 BCC 18 w/v -
  • potassium chloride BCC 0.1 M -
  • bis-tris chloride BCC 0.1 M pH 6
pH 6
100 μl of potassium chloride BCC (1 M)
62 μl of bis-tris chloride BCC pH 5.5 (1 M)
37 μl of bis-tris chloride BCC pH 6.5 (1 M)
360 μl of polyethylene glycol 4000 BCC (50 w/v)
440 μl of water (100 v/v)
C8
  • polyethylene glycol 200 BCC 18 v/v -
  • potassium chloride BCC 0.1 M -
  • dipotassium hydrogen-potassium dihydrogen phosphate BCC 0.1 M pH 7.5
pH 7.5
100 μl of potassium chloride BCC (1 M)
16 μl of dipotassium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
225 μl of polyethylene glycol 200 BCC (80 v/v)
84 μl of dipotassium hydrogen-potassium dihydrogen phosphate BCC pH 9.2 (1 M)
575 μl of water (100 v/v)
C9
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol 4000 BCC 22 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
40 μl of magnesium acetate BCC (2.5 M)
440 μl of polyethylene glycol 4000 BCC (50 w/v)
420 μl of water (100 v/v)
C10
  • calcium acetate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol 8000 BCC 22 w/v -
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
100 μl of calcium acetate BCC (1 M)
440 μl of polyethylene glycol 8000 BCC (50 w/v)
360 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 0.1 M -
  • polyethylene glycol 3350 BCC 23 w/v -
  • sodium HEPES BCC 0.1 M pH 8.5
pH 8.5
28 μl of ammonium sulfate BCC (3.5 M)
100 μl of sodium HEPES BCC pH 8.5 (1 M)
460 μl of polyethylene glycol 3350 BCC (50 w/v)
411 μl of water (100 v/v)
C12
  • polyethylene glycol 400 BCC 32 v/v -
  • potassium chloride BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
100 μl of potassium chloride BCC (1 M)
400 μl of polyethylene glycol 400 BCC (80 v/v)
400 μl of water (100 v/v)
D1
  • polyethylene glycol 300 BCC 36 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
100 μl of sodium chloride BCC (1 M)
450 μl of polyethylene glycol 300 BCC (80 v/v)
350 μl of water (100 v/v)
D2
  • polyethylene glycol monomethyl ether 550 BCC 45 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium bicine BCC 0.1 M pH 9
pH 9
529 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
12 μl of sodium bicine BCC pH 7.4 (1 M)
87 μl of sodium bicine BCC pH 9.4 (1 M)
100 μl of sodium chloride BCC (1 M)
270 μl of water (100 v/v)
D3
  • calcium chloride BCC 0.15 M -
  • polyethylene glycol 400 BCC 35 v/v -
  • glycine BCC 0.1 M pH 9
pH 9
437 μl of polyethylene glycol 400 BCC (80 v/v)
150 μl of calcium chloride BCC (1 M)
30 μl of glycine BCC pH 8.5 (1 M)
69 μl of glycine BCC pH 10 (1 M)
312 μl of water (100 v/v)
D4
  • ADA BCC 0.25 M pH 6.5
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 13 w/v -
pH 6.5
57 μl of ammonium sulfate BCC (3.5 M)
273 μl of ADA BCC pH 5.5 (0.5 M)
226 μl of ADA BCC pH 7.5 (0.5 M)
260 μl of polyethylene glycol 4000 BCC (50 w/v)
182 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 7.5
  • polyethylene glycol monomethyl ether 2000 BCC 14 w/v -
  • choline chloride BCC 0.2 M -
pH 7.5
200 μl of choline chloride BCC (1 M)
84 μl of tris chloride BCC pH 7 (1 M)
280 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
420 μl of water (100 v/v)
D6
  • polyethylene glycol 6000 BCC 19 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
380 μl of polyethylene glycol 6000 BCC (50 w/v)
63 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36 μl of sodium MOPS BCC pH 8.2 (0.5 M)
200 μl of sodium chloride BCC (1 M)
320 μl of water (100 v/v)
D7
  • polyethylene glycol 6000 BCC 19 w/v -
  • sodium chloride BCC 0.05 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
380 μl of polyethylene glycol 6000 BCC (50 w/v)
63 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36 μl of sodium MOPS BCC pH 8.2 (0.5 M)
50 μl of sodium chloride BCC (1 M)
470 μl of water (100 v/v)
D8
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 19 w/v -
  • tris chloride BCC 0.05 M pH 8
pH 8
380 μl of polyethylene glycol 3350 BCC (50 w/v)
27 μl of tris chloride BCC pH 7 (1 M)
22 μl of tris chloride BCC pH 9 (1 M)
200 μl of magnesium formate BCC (1 M)
370 μl of water (100 v/v)
D9
  • calcium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 5.2
  • polyethylene glycol monomethyl ether 350 BCC 20 v/v -
pH 5.2
100 μl of sodium MES BCC pH 5.2 (1 M)
200 μl of calcium chloride BCC (1 M)
250 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
450 μl of water (100 v/v)
D10
  • ammonium nitrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium HEPES BCC 0.05 M pH 7
pH 7
200 μl of ammonium nitrate BCC (1 M)
40 μl of sodium HEPES BCC pH 6.5 (1 M)
9 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
350 μl of water (100 v/v)
D11
  • lithium chloride BCC 0.02 M -
  • magnesium chloride BCC 0.5 M -
  • polyethylene glycol 1000 BCC 33 w/v -
  • glycine BCC 0.02 M pH 10
pH 10
20 μl of lithium chloride BCC (1 M)
660 μl of polyethylene glycol 1000 BCC (50 w/v)
0 μl of glycine BCC pH 8.5 (1 M)
19 μl of glycine BCC pH 10 (1 M)
200 μl of magnesium chloride BCC (2.5 M)
100 μl of water (100 v/v)
D12
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 400 BCC 24 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of calcium acetate BCC (1 M)
400 μl of water (100 v/v)
E1
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium acetate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
350 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of sodium acetate BCC (1 M)
350 μl of water (100 v/v)
E2
  • calcium acetate BCC 0.05 M -
  • polyethylene glycol 400 BCC 29 v/v -
  • sodium chloride BCC 0.2 M -
NA
362 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of calcium acetate BCC (1 M)
200 μl of sodium chloride BCC (1 M)
387 μl of water (100 v/v)
E3
  • polyethylene glycol 400 BCC 29 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
362 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of sodium chloride BCC (1 M)
337 μl of water (100 v/v)
E4
  • tris chloride BCC 0.1 M pH 7
  • pentaerythritol ethoxylate (15/4 EO/OH) BCC 31 w/v -
  • ammonium formate BCC 0.2 M -
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
200 μl of ammonium formate BCC (1 M)
620 μl of pentaerythritol ethoxylate (15/4 EO/OH) BCC (50 w/v)
80 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 35 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
57 μl of ammonium sulfate BCC (3.5 M)
700 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
142 μl of water (100 v/v)
E6
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 400 BCC 38 v/v -
  • sodium acetate BCC 0.1 M -
NA
475 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of calcium acetate BCC (1 M)
100 μl of sodium acetate BCC (1 M)
225 μl of water (100 v/v)
E7
  • polyethylene glycol 400 BCC 38 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium MOPS BCC 0.1 M pH 7.5
pH 7.5
475 μl of polyethylene glycol 400 BCC (80 v/v)
59 μl of sodium MOPS BCC pH 6.2 (0.5 M)
140 μl of sodium MOPS BCC pH 8.2 (0.5 M)
200 μl of sodium chloride BCC (1 M)
125 μl of water (100 v/v)
E8
  • ammonium sulfate BCC 2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
571 μl of ammonium sulfate BCC (3.5 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
200 μl of sodium chloride BCC (1 M)
128 μl of water (100 v/v)
E9
  • ammonium sulfate BCC 0.225 M -
  • polyethylene glycol 4000 BCC 12 w/v -
  • sodium acetate BCC 0.05 M -
NA
64 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium acetate BCC (1 M)
240 μl of polyethylene glycol 4000 BCC (50 w/v)
645 μl of water (100 v/v)
E10
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium acetate BCC 0.05 M -
  • sodium chloride BCC 0.23 M -
NA
412 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium acetate BCC (1 M)
230 μl of sodium chloride BCC (1 M)
307 μl of water (100 v/v)
E11
  • magnesium formate BCC 0.25 M -
  • polyethylene glycol 3000 BCC 22 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
440 μl of polyethylene glycol 3000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
250 μl of magnesium formate BCC (1 M)
210 μl of water (100 v/v)
E12
  • magnesium chloride BCC 0.25 M -
  • polyethylene glycol 1000 BCC 40 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
800 μl of polyethylene glycol 1000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
100 μl of magnesium chloride BCC (2.5 M)
F1
  • lithium sulfate BCC 0.3 M -
  • polyethylene glycol 400 BCC 25 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
312 μl of polyethylene glycol 400 BCC (80 v/v)
300 μl of lithium sulfate BCC (1 M)
287 μl of water (100 v/v)
F2
  • polyethylene glycol monomethyl ether 550 BCC 33 v/v -
  • tris chloride BCC 0.05 M pH 9
  • ammonium formate BCC 0.3 M -
pH 9
50 μl of tris chloride BCC pH 9 (1 M)
388 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
300 μl of ammonium formate BCC (1 M)
261 μl of water (100 v/v)
F3
  • barium chloride BCC 0.3 M -
  • polyethylene glycol 400 BCC 34 v/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
425 μl of polyethylene glycol 400 BCC (80 v/v)
300 μl of barium chloride BCC (1 M)
175 μl of water (100 v/v)
F4
  • lithium chloride BCC 0.32 M -
  • polyethylene glycol 4000 BCC 14 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
320 μl of lithium chloride BCC (1 M)
280 μl of polyethylene glycol 4000 BCC (50 w/v)
300 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 0.34 M -
  • polyethylene glycol 4000 BCC 12 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
97 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
240 μl of polyethylene glycol 4000 BCC (50 w/v)
562 μl of water (100 v/v)
F6
  • lithium sulfate BCC 0.35 M -
  • polyethylene glycol 600 BCC 11 w/v -
  • sodium acetate BCC 0.1 M -
NA
100 μl of sodium acetate BCC (1 M)
220 μl of polyethylene glycol 600 BCC (50 w/v)
350 μl of lithium sulfate BCC (1 M)
330 μl of water (100 v/v)
F7
  • polyethylene glycol 400 BCC 22 v/v -
  • potassium nitrate BCC 0.37 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
275 μl of polyethylene glycol 400 BCC (80 v/v)
370 μl of potassium nitrate BCC (1 M)
255 μl of water (100 v/v)
F8
  • ammonium sulfate BCC 0.4 M -
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
114 μl of ammonium sulfate BCC (3.5 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of polyethylene glycol 3350 BCC (50 w/v)
585 μl of water (100 v/v)
F9
  • magnesium chloride BCC 0.04 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium chloride BCC 0.05 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 400 BCC (80 v/v)
16 μl of magnesium chloride BCC (2.5 M)
50 μl of sodium chloride BCC (1 M)
434 μl of water (100 v/v)
F10
  • polyethylene glycol 400 BCC 12 v/v -
  • potassium chloride BCC 0.4 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
400 μl of potassium chloride BCC (1 M)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
150 μl of polyethylene glycol 400 BCC (80 v/v)
400 μl of water (100 v/v)
F11
  • ammonium nitrate BCC 0.4 M -
  • polyethylene glycol 4000 BCC 15 w/v -
  • sodium acetate BCC 0.1 M -
NA
400 μl of ammonium nitrate BCC (1 M)
100 μl of sodium acetate BCC (1 M)
300 μl of polyethylene glycol 4000 BCC (50 w/v)
200 μl of water (100 v/v)
F12
  • polyethylene glycol 4000 BCC 16 w/v -
  • sodium acetate BCC 0.1 M -
  • sodium thiocyanate BCC 0.4 M -
NA
200 μl of sodium thiocyanate BCC (2 M)
100 μl of sodium acetate BCC (1 M)
320 μl of polyethylene glycol 4000 BCC (50 w/v)
380 μl of water (100 v/v)
G1
  • polyethylene glycol 400 BCC 20 v/v -
  • potassium chloride BCC 0.5 M -
  • sodium HEPES BCC 0.05 M pH 6.5
pH 6.5
500 μl of potassium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of water (100 v/v)
G2
  • magnesium chloride BCC 0.5 M -
  • tris chloride BCC 0.05 M pH 7.5
  • polyethylene glycol monomethyl ether 350 BCC 21 v/v -
pH 7.5
42 μl of tris chloride BCC pH 7 (1 M)
262 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
7 μl of tris chloride BCC pH 9 (1 M)
200 μl of magnesium chloride BCC (2.5 M)
487 μl of water (100 v/v)
G3
  • polyethylene glycol 4000 BCC 11 w/v -
  • potassium formate BCC 0.8 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
160 μl of potassium formate BCC (5 M)
220 μl of polyethylene glycol 4000 BCC (50 w/v)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
520 μl of water (100 v/v)
G4
  • polyethylene glycol 8000 BCC 9 w/v -
  • sodium MOPS BCC 0.1 M pH 7
pH 7
127 μl of sodium MOPS BCC pH 6.2 (0.5 M)
72 μl of sodium MOPS BCC pH 8.2 (0.5 M)
180 μl of polyethylene glycol 8000 BCC (50 w/v)
620 μl of water (100 v/v)
G5
  • polyethylene glycol 20000 BCC 11 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
440 μl of polyethylene glycol 20000 BCC (25 w/v)
460 μl of water (100 v/v)
G6
  • polyethylene glycol 400 BCC 13 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
162 μl of polyethylene glycol 400 BCC (80 v/v)
737 μl of water (100 v/v)
G7
  • ADA BCC 0.1 M pH 5.5
  • polyethylene glycol 6000 BCC 14 w/v -
pH 5.5
280 μl of polyethylene glycol 6000 BCC (50 w/v)
200 μl of ADA BCC pH 5.5 (0.5 M)
520 μl of water (100 v/v)
G8
  • tris chloride BCC 0.05 M pH 7.5
  • polyethylene glycol monomethyl ether 350 BCC 17 v/v -
pH 7.5
42 μl of tris chloride BCC pH 7 (1 M)
212 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
7 μl of tris chloride BCC pH 9 (1 M)
737 μl of water (100 v/v)
G9
  • polyethylene glycol 300 BCC 22 v/v -
  • trisodium citrate-citric acid BCC 0.07 M pH 4.5
pH 4.5
30 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
39 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
275 μl of polyethylene glycol 300 BCC (80 v/v)
655 μl of water (100 v/v)
G10
  • ADA BCC 0.05 M pH 6.5
  • polyethylene glycol 400 BCC 24 v/v -
pH 6.5
300 μl of polyethylene glycol 400 BCC (80 v/v)
54 μl of ADA BCC pH 5.5 (0.5 M)
45 μl of ADA BCC pH 7.5 (0.5 M)
600 μl of water (100 v/v)
G11
  • polyethylene glycol 1500 BCC 24 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
480 μl of polyethylene glycol 1500 BCC (50 w/v)
420 μl of water (100 v/v)
G12
  • polyethylene glycol 600 BCC 28 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
560 μl of polyethylene glycol 600 BCC (50 w/v)
340 μl of water (100 v/v)
H1
  • polyethylene glycol 400 BCC 28 v/v -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
350 μl of polyethylene glycol 400 BCC (80 v/v)
10 μl of tris chloride BCC pH 7 (1 M)
39 μl of tris chloride BCC pH 9 (1 M)
600 μl of water (100 v/v)
H2
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium bicine BCC 0.1 M pH 9
pH 9
375 μl of polyethylene glycol 400 BCC (80 v/v)
12 μl of sodium bicine BCC pH 7.4 (1 M)
87 μl of sodium bicine BCC pH 9.4 (1 M)
525 μl of water (100 v/v)
H3
  • ADA BCC 0.1 M pH 7
  • polyethylene glycol 600 BCC 31 w/v -
pH 7
42 μl of ADA BCC pH 5.5 (0.5 M)
157 μl of ADA BCC pH 7.5 (0.5 M)
620 μl of polyethylene glycol 600 BCC (50 w/v)
180 μl of water (100 v/v)
H4
  • polyethylene glycol monomethyl ether 550 BCC 32 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
376 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
523 μl of water (100 v/v)
H5
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
487 μl of water (100 v/v)
H6
  • polyethylene glycol 3350 BCC 34 w/v -
  • trisodium citrate-citric acid BCC 0.18 M pH 4
pH 4
104 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
75 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
680 μl of polyethylene glycol 3350 BCC (50 w/v)
140 μl of water (100 v/v)
H7
  • polyethylene glycol 200 BCC 44 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
550 μl of polyethylene glycol 200 BCC (80 v/v)
350 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
45 μl of tris chloride BCC pH 9 (1 M)
250 μl of water (100 v/v)
H9
  • ammonium chloride BCC 2.75 M -
  • bis-tris chloride BCC 0.025 M pH 7
pH 7
550 μl of ammonium chloride BCC (5 M)
8 μl of bis-tris chloride BCC pH 6.5 (1 M)
16 μl of bis-tris chloride BCC pH 7.5 (1 M)
425 μl of water (100 v/v)
H10
  • ammonium chloride BCC 2.8 M -
  • sodium HEPES BCC 0.075 M pH 7.5
pH 7.5
560 μl of ammonium chloride BCC (5 M)
37 μl of sodium HEPES BCC pH 6.5 (1 M)
37 μl of sodium HEPES BCC pH 8.5 (1 M)
365 μl of water (100 v/v)
H11
  • ammonium sulfate BCC 3 M -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
857 μl of ammonium sulfate BCC (3.5 M)
90 μl of sodium MES BCC pH 5.2 (1 M)
10 μl of sodium MES BCC pH 7.2 (1 M)
42 μl of water (100 v/v)
H12
  • 1,6-hexanediol BCC 3.25 M -
  • sodium HEPES BCC 0.01 M pH 7.5
pH 7.5
650 μl of 1,6-hexanediol BCC (5 M)
5 μl of sodium HEPES BCC pH 6.5 (1 M)
5 μl of sodium HEPES BCC pH 8.5 (1 M)
340 μl of water (100 v/v)