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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
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Supplemental Information
Screen Description
MemGold_C3
BCC Core Screens / MemGold_C3
Created 2025-04-10.
Based on Molecular Dimensions MemGold screen, made up by C3, and modified to have only 80 stocks
Export
MemGold_C3
Barcodes
MemGold_C3
- Z0002497
- Z0002766
- Z0002767
Screen Structure
MemGold_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 59 |
Internal diversity (score mean dist) | 0.927 |
Screen Composition
ingredient type | MemGold_C3 |
---|---|
Buffer | 98 |
Cryoprotectant | 2 |
Organic (non-volatile) | 4 |
Polymer | 89 |
Salt | 120 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.2 | 22 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 628 μl of ammonium sulfate BCC (3.5 M) 57 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 291 μl of water (100 v/v) |
A2 |
| pH 8 | 750 μl of trisodium citrate BCC (1.6 M) 5 μl of tris chloride BCC pH 7 (1 M) 4 μl of tris chloride BCC pH 9 (1 M) 240 μl of water (100 v/v) |
A3 |
| pH 8.5 | 10 μl of tricine BCC pH 9.2 (1 M) 4 μl of tricine BCC pH 7.2 (1 M) 480 μl of polyethylene glycol 4000 BCC (50 w/v) 505 μl of water (100 v/v) |
A4 |
| pH 7 | 72 μl of sodium chloride BCC (5 M) 5 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 198 μl of polyethylene glycol 4000 BCC (50 w/v) 9 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 715 μl of water (100 v/v) |
A5 |
| pH 8 | 60 μl of sodium chloride BCC (5 M) 5 μl of tris chloride BCC pH 7 (1 M) 4 μl of tris chloride BCC pH 9 (1 M) 550 μl of polyethylene glycol 4000 BCC (50 w/v) 380 μl of water (100 v/v) |
A6 |
| NA | 24 μl of sodium MES BCC pH 5.2 (1 M) 88 μl of sodium MES BCC pH 7.2 (1 M) 113 μl of bis-tris chloride BCC pH 6.5 (1 M) 132 μl of polyethylene glycol 6000 BCC (50 w/v) 642 μl of water (100 v/v) |
A7 |
| pH 7.5 | 275 μl of glycerol BCC (80 v/v) 28 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 240 μl of polyethylene glycol 4000 BCC (50 w/v) 356 μl of water (100 v/v) |
A8 |
| pH 6.5 | 154 μl of polyethylene glycol 1500 BCC (50 w/v) 10 μl of magnesium sulfate BCC (1 M) 4 μl of sodium chloride BCC (5 M) 5 μl of sodium MES BCC pH 5.2 (1 M) 14 μl of sodium MES BCC pH 7.2 (1 M) 20 μl of calcium chloride BCC (1 M) 792 μl of water (100 v/v) |
A9 |
| pH 7.5 | 714 μl of ammonium sulfate BCC (3.5 M) 25 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of sodium HEPES BCC pH 6.5 (1 M) 235 μl of water (100 v/v) |
A10 |
| pH 7.5 | 687 μl of trisodium citrate BCC (1.6 M) 33 μl of sodium HEPES BCC pH 8.5 (1 M) 33 μl of sodium HEPES BCC pH 6.5 (1 M) 245 μl of water (100 v/v) |
A11 |
| NA | 75 μl of dipotassium hydrogen phosphate BCC (2 M) 857 μl of ammonium sulfate BCC (3.5 M) 67 μl of water (100 v/v) |
A12 |
| pH 5.8 | 700 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 14 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 40 μl of magnesium acetate BCC (2.5 M) 86 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 160 μl of water (100 v/v) |
B1 |
| pH 5.6 | 20 μl of sodium chloride BCC (5 M) 220 μl of polyethylene glycol 3350 BCC (50 w/v) 3 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 16 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 740 μl of water (100 v/v) |
B2 |
| pH 5.6 | 20 μl of sodium chloride BCC (5 M) 110 μl of polyethylene glycol 3350 BCC (50 w/v) 3 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 16 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 850 μl of water (100 v/v) |
B3 |
| pH 8.2 | 50 μl of barium chloride BCC (1 M) 400 μl of polyethylene glycol 400 BCC (80 v/v) 40 μl of tris chloride BCC pH 7 (1 M) 59 μl of tris chloride BCC pH 9 (1 M) 50 μl of calcium chloride BCC (1 M) 400 μl of water (100 v/v) |
B4 |
| pH 6.2 | 10 μl of sodium chloride BCC (5 M) 80 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 320 μl of polyethylene glycol 4000 BCC (50 w/v) 20 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 570 μl of water (100 v/v) |
B5 |
| pH 8.2 | 100 μl of magnesium chloride BCC (1 M) 12 μl of tris chloride BCC pH 7 (1 M) 17 μl of tris chloride BCC pH 9 (1 M) 380 μl of polyethylene glycol 4000 BCC (50 w/v) 490 μl of water (100 v/v) |
B6 |
| pH 7.5 | 40 μl of sodium chloride BCC (5 M) 12 μl of sodium HEPES BCC pH 8.5 (1 M) 12 μl of sodium HEPES BCC pH 6.5 (1 M) 260 μl of polyethylene glycol 4000 BCC (50 w/v) 675 μl of water (100 v/v) |
B7 |
| pH 7.5 | 220 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 680 μl of water (100 v/v) |
B8 |
| pH 6.7 | 16 μl of sodium chloride BCC (5 M) 3 μl of sodium MES BCC pH 5.2 (1 M) 6 μl of potassium chloride BCC (3 M) 16 μl of sodium MES BCC pH 7.2 (1 M) 132 μl of polyethylene glycol 4000 BCC (50 w/v) 825 μl of water (100 v/v) |
B9 |
| pH 7 | 33 μl of potassium chloride BCC (3 M) 20 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 546 μl of water (100 v/v) |
B10 |
| pH 6.7 | 132 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of magnesium chloride BCC (1 M) 22 μl of sodium cacodylate BCC pH 5.3 (1 M) 77 μl of sodium cacodylate BCC pH 7.3 (1 M) 718 μl of water (100 v/v) |
B11 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 66 μl of potassium chloride BCC (3 M) 740 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 93 μl of water (100 v/v) |
B12 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 110 μl of polyethylene glycol 4000 BCC (50 w/v) 790 μl of water (100 v/v) |
C1 |
| pH 7 | 20 μl of sodium chloride BCC (5 M) 20 μl of tris chloride BCC pH 7 (1 M) 154 μl of polyethylene glycol 4000 BCC (50 w/v) 806 μl of water (100 v/v) |
C2 |
| pH 7.5 | 275 μl of polyethylene glycol 400 BCC (80 v/v) 100 μl of magnesium chloride BCC (1 M) 84 μl of tris chloride BCC pH 7 (1 M) 15 μl of tris chloride BCC pH 9 (1 M) 525 μl of water (100 v/v) |
C3 |
| pH 8 | 8 μl of sodium chloride BCC (5 M) 337 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 21 μl of tris chloride BCC pH 7 (1 M) 18 μl of tris chloride BCC pH 9 (1 M) 614 μl of water (100 v/v) |
C4 |
| pH 6 | 10 μl of sodium chloride BCC (5 M) 10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 275 μl of polyethylene glycol 400 BCC (80 v/v) 20 μl of magnesium chloride BCC (1 M) 90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 595 μl of water (100 v/v) |
C5 |
| pH 5.5 | 176 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 724 μl of water (100 v/v) |
C6 |
| NA | 260 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 80 μl of ammonium acetate BCC (5 M) 660 μl of water (100 v/v) |
C7 |
| pH 7 | 300 μl of polyethylene glycol 2000 BCC (50 w/v) 7 μl of bis-tris chloride BCC pH 6.5 (1 M) 12 μl of bis-tris chloride BCC pH 7.5 (1 M) 680 μl of water (100 v/v) |
C8 |
| pH 7.5 | 220 μl of polyethylene glycol 1500 BCC (50 w/v) 20 μl of sodium chloride BCC (5 M) 100 μl of magnesium chloride BCC (1 M) 16 μl of tris chloride BCC pH 7 (1 M) 3 μl of tris chloride BCC pH 9 (1 M) 640 μl of water (100 v/v) |
C9 |
| pH 8 | 220 μl of polyethylene glycol 1500 BCC (50 w/v) 20 μl of sodium chloride BCC (5 M) 100 μl of magnesium chloride BCC (1 M) 81 μl of sodium HEPES BCC pH 8.5 (1 M) 18 μl of sodium HEPES BCC pH 6.5 (1 M) 560 μl of water (100 v/v) |
C10 |
| pH 7 | 66 μl of potassium chloride BCC (3 M) 440 μl of polyethylene glycol 3000 BCC (50 w/v) 200 μl of sodium acetate BCC (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 193 μl of water (100 v/v) |
C11 |
| pH 7 | 660 μl of jeffamine M-600 BCC pH 7 (50 v/v) 1 μl of sodium HEPES BCC pH 8.5 (1 M) 20 μl of nickel(II) sulfate BCC (1 M) 8 μl of sodium HEPES BCC pH 6.5 (1 M) 310 μl of water (100 v/v) |
C12 |
| pH 8 | 30 μl of sodium chloride BCC (5 M) 54 μl of tris chloride BCC pH 7 (1 M) 260 μl of polyethylene glycol 6000 BCC (50 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 610 μl of water (100 v/v) |
D1 |
| pH 7.5 | 662 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of calcium chloride BCC (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 37 μl of water (100 v/v) |
D2 |
| pH 5 | 350 μl of polyethylene glycol 400 BCC (80 v/v) 20 μl of magnesium acetate BCC (2.5 M) 17 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 32 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 580 μl of water (100 v/v) |
D3 |
| pH 7.5 | 25 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of sodium HEPES BCC pH 6.5 (1 M) 440 μl of polyethylene glycol 4000 BCC (50 w/v) 510 μl of water (100 v/v) |
D4 |
| pH 8 | 550 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 200 μl of calcium chloride BCC (1 M) 150 μl of water (100 v/v) |
D5 |
| pH 5.4 | 300 μl of polyethylene glycol 400 BCC (80 v/v) 20 μl of magnesium acetate BCC (2.5 M) 6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 43 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 630 μl of water (100 v/v) |
D6 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 325 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of calcium chloride BCC (1 M) 375 μl of water (100 v/v) |
D7 |
| pH 8.5 | 487 μl of polyethylene glycol 400 BCC (80 v/v) 33 μl of potassium chloride BCC (3 M) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 379 μl of water (100 v/v) |
D8 |
| pH 9 | 275 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of magnesium chloride BCC (1 M) 30 μl of glycine BCC pH 8.5 (1 M) 69 μl of glycine BCC pH 10 (1 M) 575 μl of water (100 v/v) |
D9 |
| pH 3.8 | 28 μl of ammonium sulfate BCC (3.5 M) 100 μl of glycine BCC pH 3.8 (1 M) 280 μl of triethylene glycol BCC (100 v/v) 591 μl of water (100 v/v) |
D10 |
| pH 7.2 | 360 μl of polyethylene glycol 3350 BCC (50 w/v) 29 μl of sodium HEPES BCC pH 8.5 (1 M) 37 μl of sodium formate BCC (4 M) 70 μl of sodium HEPES BCC pH 6.5 (1 M) 502 μl of water (100 v/v) |
D11 |
| NA | 200 μl of sodium acetate BCC (1 M) 176 μl of polyethylene glycol 6000 BCC (50 w/v) 624 μl of water (100 v/v) |
D12 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 66 μl of potassium chloride BCC (3 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 360 μl of polyethylene glycol 6000 BCC (50 w/v) 473 μl of water (100 v/v) |
E1 |
| pH 8 | 437 μl of polyethylene glycol 400 BCC (80 v/v) 137 μl of trisodium citrate BCC (1.6 M) 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 325 μl of water (100 v/v) |
E2 |
| pH 4.5 | 212 μl of polyethylene glycol 400 BCC (80 v/v) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 687 μl of water (100 v/v) |
E3 |
| pH 8.5 | 4 μl of tris chloride BCC pH 7 (1 M) 400 μl of lithium sulfate BCC (2.5 M) 36 μl of polyethylene glycol 8000 BCC (50 w/v) 15 μl of tris chloride BCC pH 9 (1 M) 544 μl of water (100 v/v) |
E4 |
| pH 7.5 | 16 μl of tris chloride BCC pH 7 (1 M) 258 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 3 μl of tris chloride BCC pH 9 (1 M) 721 μl of water (100 v/v) |
E5 |
| pH 10 | 10 μl of sodium chloride BCC (5 M) 0 μl of glycine BCC pH 8.5 (1 M) 19 μl of glycine BCC pH 10 (1 M) 660 μl of polyethylene glycol 1000 BCC (50 w/v) 310 μl of water (100 v/v) |
E6 |
| pH 8.5 | 200 μl of magnesium chloride BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 200 μl of water (100 v/v) |
E7 |
| pH 6.5 | 200 μl of magnesium chloride BCC (1 M) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 620 μl of polyethylene glycol 2000 BCC (50 w/v) 80 μl of water (100 v/v) |
E8 |
| pH 4.6 | 360 μl of polyethylene glycol 3350 BCC (50 w/v) 397 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 242 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) |
E9 |
| pH 8 | 20 μl of sodium chloride BCC (5 M) 412 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 100 μl of cadmium chloride BCC (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 367 μl of water (100 v/v) |
E10 |
| pH 8.9 | 620 μl of polyethylene glycol 2000 BCC (50 w/v) 16 μl of sodium bicine BCC pH 7.4 (1 M) 83 μl of sodium bicine BCC pH 9.4 (1 M) 280 μl of water (100 v/v) |
E11 |
| pH 8.5 | 437 μl of polyethylene glycol 400 BCC (80 v/v) 10 μl of tris chloride BCC pH 7 (1 M) 20 μl of lithium sulfate BCC (2.5 M) 39 μl of tris chloride BCC pH 9 (1 M) 41 μl of sodium sulfate BCC (1.2 M) 450 μl of water (100 v/v) |
E12 |
| pH 9.5 | 20 μl of sodium chloride BCC (5 M) 4 μl of glycine BCC pH 8.5 (1 M) 45 μl of glycine BCC pH 10 (1 M) 412 μl of polyethylene glycol 300 BCC (80 v/v) 517 μl of water (100 v/v) |
F1 |
| pH 8 | 460 μl of polyethylene glycol 2000 BCC (50 w/v) 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 300 μl of magnesium nitrate BCC (1 M) 140 μl of water (100 v/v) |
F2 |
| NA | 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 16 μl of tris chloride BCC pH 7 (1 M) 48 μl of lithium sulfate BCC (2.5 M) 3 μl of tris chloride BCC pH 9 (1 M) 250 μl of polyethylene glycol 300 BCC (80 v/v) 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 582 μl of water (100 v/v) |
F3 |
| pH 9 | 20 μl of sodium chloride BCC (5 M) 250 μl of polyethylene glycol 400 BCC (80 v/v) 120 μl of tris chloride BCC pH 9 (1 M) 610 μl of water (100 v/v) |
F4 |
| pH 7 | 40 μl of sodium chloride BCC (5 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 258 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 601 μl of water (100 v/v) |
F5 |
| pH 8 | 20 μl of sodium chloride BCC (5 M) 262 μl of polyethylene glycol 400 BCC (80 v/v) 325 μl of sodium acetate BCC (1 M) 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 292 μl of water (100 v/v) |
F6 |
| pH 6.2 | 64 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 360 μl of polyethylene glycol 2000 BCC (50 w/v) 12 μl of trisodium citrate BCC (1.6 M) 16 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 547 μl of water (100 v/v) |
F7 |
| NA | 30 μl of tripotassium citrate BCC (1 M) 20 μl of potassium nitrate BCC (1 M) 154 μl of polyethylene glycol 4000 BCC (50 w/v) 796 μl of water (100 v/v) |
F8 |
| pH 8.5 | 20 μl of sodium chloride BCC (5 M) 5 μl of magnesium chloride BCC (1 M) 600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 275 μl of water (100 v/v) |
F9 |
| pH 7 | 412 μl of polyethylene glycol 400 BCC (80 v/v) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of calcium chloride BCC (1 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 287 μl of water (100 v/v) |
F10 |
| pH 7 | 260 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 100 μl of tris chloride BCC pH 7 (1 M) 100 μl of calcium chloride BCC (1 M) 540 μl of water (100 v/v) |
F11 |
| pH 4 | 4 μl of sodium chloride BCC (5 M) 18 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 1 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 412 μl of polyethylene glycol 200 BCC (80 v/v) 506 μl of water (100 v/v) |
F12 |
| pH 4.5 | 14 μl of sodium chloride BCC (5 M) 220 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 275 μl of polyethylene glycol 400 BCC (80 v/v) 280 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 211 μl of water (100 v/v) |
G1 |
| pH 4.6 | 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 329 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 513 μl of water (100 v/v) |
G2 |
| pH 9 | 687 μl of polyethylene glycol 400 BCC (80 v/v) 15 μl of glycine BCC pH 8.5 (1 M) 34 μl of glycine BCC pH 10 (1 M) 262 μl of water (100 v/v) |
G3 |
| pH 8.5 | 20 μl of sodium chloride BCC (5 M) 412 μl of polyethylene glycol 400 BCC (80 v/v) 100 μl of magnesium chloride BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 367 μl of water (100 v/v) |
G4 |
| pH 4 | 108 μl of phosphate-citrate BCC pH 9.3 (1 M) 92 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 40 μl of lithium sulfate BCC (2.5 M) 380 μl of polyethylene glycol 1000 BCC (50 w/v) 380 μl of water (100 v/v) |
G5 |
| pH 4 | 14 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 412 μl of polyethylene glycol 400 BCC (80 v/v) 200 μl of magnesium chloride BCC (1 M) 33 μl of potassium chloride BCC (3 M) 10 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 329 μl of water (100 v/v) |
G6 |
| pH 6.1 | 27 μl of sodium MES BCC pH 5.2 (1 M) 50 μl of zinc acetate BCC (1 M) 22 μl of sodium MES BCC pH 7.2 (1 M) 220 μl of polyethylene glycol 8000 BCC (50 w/v) 680 μl of water (100 v/v) |
G7 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 440 μl of polyethylene glycol 8000 BCC (50 w/v) 45 μl of tris chloride BCC pH 9 (1 M) 300 μl of magnesium nitrate BCC (1 M) 160 μl of water (100 v/v) |
G8 |
| pH 6.5 | 20 μl of sodium chloride BCC (5 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 412 μl of polyethylene glycol 400 BCC (80 v/v) 40 μl of ethylene glycol BCC (100 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 427 μl of water (100 v/v) |
G9 |
| pH 5.5 | 10 μl of sodium chloride BCC (5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 325 μl of polyethylene glycol 400 BCC (80 v/v) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 565 μl of water (100 v/v) |
G10 |
| pH 9.3 | 375 μl of polyethylene glycol 400 BCC (80 v/v) 15 μl of glycine BCC pH 8.5 (1 M) 85 μl of glycine BCC pH 10 (1 M) 40 μl of lithium sulfate BCC (2.5 M) 485 μl of water (100 v/v) |
G11 |
| NA | 440 μl of polyethylene glycol 6000 BCC (50 w/v) 150 μl of tripotassium citrate BCC (1 M) 100 μl of sodium dihydrogen phosphate BCC (1 M) 50 μl of trilithium citrate BCC (1 M) 260 μl of water (100 v/v) |
G12 |
| pH 7.8 | 35 μl of sodium HEPES BCC pH 8.5 (1 M) 10 μl of zinc sulfate BCC (0.1 M) 560 μl of polyethylene glycol 600 BCC (50 w/v) 14 μl of sodium HEPES BCC pH 6.5 (1 M) 380 μl of water (100 v/v) |
H1 |
| pH 7 | 20 μl of sodium chloride BCC (5 M) 36 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 412 μl of polyethylene glycol 300 BCC (80 v/v) 64 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 467 μl of water (100 v/v) |
H2 |
| pH 9 | 20 μl of sodium chloride BCC (5 M) 6 μl of sodium bicine BCC pH 7.4 (1 M) 43 μl of sodium bicine BCC pH 9.4 (1 M) 412 μl of polyethylene glycol 300 BCC (80 v/v) 517 μl of water (100 v/v) |
H3 |
| pH 6 | 50 μl of zinc acetate BCC (1 M) 60 μl of ethylene glycol BCC (100 v/v) 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 132 μl of polyethylene glycol 8000 BCC (50 w/v) 658 μl of water (100 v/v) |
H4 |
| pH 3.5 | 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 350 μl of polyethylene glycol 400 BCC (80 v/v) 80 μl of lithium sulfate BCC (2.5 M) 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 470 μl of water (100 v/v) |
H5 |
| pH 7.5 | 20 μl of sodium chloride BCC (5 M) 84 μl of tris chloride BCC pH 7 (1 M) 15 μl of tris chloride BCC pH 9 (1 M) 220 μl of polyethylene glycol 4000 BCC (50 w/v) 660 μl of water (100 v/v) |
H6 |
| pH 7.4 | 140 μl of polyethylene glycol 3000 BCC (50 w/v) 6 μl of tricine BCC pH 9.2 (1 M) 20 μl of lithium sulfate BCC (2.5 M) 93 μl of tricine BCC pH 7.2 (1 M) 740 μl of water (100 v/v) |
H7 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 412 μl of polyethylene glycol 400 BCC (80 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of calcium chloride BCC (1 M) 287 μl of water (100 v/v) |
H8 |
| pH 6 | 200 μl of sodium chloride BCC (5 M) 10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 560 μl of polyethylene glycol 4000 BCC (50 w/v) 90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 140 μl of water (100 v/v) |
H9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 220 μl of polyethylene glycol 4000 BCC (50 w/v) 680 μl of water (100 v/v) |
H10 |
| pH 7 | 14 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 20 μl of zinc sulfate BCC (0.1 M) 65 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of water (100 v/v) |
H11 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 30 μl of magnesium chloride BCC (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 495 μl of water (100 v/v) |
H12 |
| pH 7 | 600 μl of sodium chloride BCC (5 M) 78 μl of bis-tris propane chloride BCC pH 6 (1 M) 22 μl of bis-tris propane chloride BCC pH 10 (1 M) 300 μl of water (100 v/v) |