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Supplemental Information

Screen Description

MemGold_C3

BCC Core Screens / MemGold_C3
Created 2025-04-10.
Based on Molecular Dimensions MemGold screen, made up by C3, and modified to have only 80 stocks

Export

Barcodes

MemGold_C3

  • Z0002497
  • Z0002766
  • Z0002767

Screen Structure

MemGold_C3

Well Count 96
Number of distinct ingredients 59
Internal diversity (score mean dist) 0.927

Screen Composition

2025-06-12T23:10:40.456210 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type MemGold_C3
Buffer 98
Cryoprotectant 2
Organic (non-volatile) 4
Polymer 89
Salt 120

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemGold_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 2.2 M -
  • trisodium citrate-citric acid BCC 0.08 M pH 5.2
pH 5.2
22 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
628 μl of ammonium sulfate BCC (3.5 M)
57 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
291 μl of water (100 v/v)
A2
  • tris chloride BCC 0.01 M pH 8
  • trisodium citrate BCC 1.2 M -
pH 8
750 μl of trisodium citrate BCC (1.6 M)
5 μl of tris chloride BCC pH 7 (1 M)
4 μl of tris chloride BCC pH 9 (1 M)
240 μl of water (100 v/v)
A3
  • polyethylene glycol 4000 BCC 24 w/v -
  • tricine BCC 0.015 M pH 8.5
pH 8.5
10 μl of tricine BCC pH 9.2 (1 M)
4 μl of tricine BCC pH 7.2 (1 M)
480 μl of polyethylene glycol 4000 BCC (50 w/v)
505 μl of water (100 v/v)
A4
  • polyethylene glycol 4000 BCC 9.9 w/v -
  • sodium chloride BCC 0.36 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.015 M pH 7
pH 7
72 μl of sodium chloride BCC (5 M)
5 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
198 μl of polyethylene glycol 4000 BCC (50 w/v)
9 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
715 μl of water (100 v/v)
A5
  • polyethylene glycol 4000 BCC 27.5 w/v -
  • sodium chloride BCC 0.3 M -
  • tris chloride BCC 0.01 M pH 8
pH 8
60 μl of sodium chloride BCC (5 M)
5 μl of tris chloride BCC pH 7 (1 M)
4 μl of tris chloride BCC pH 9 (1 M)
550 μl of polyethylene glycol 4000 BCC (50 w/v)
380 μl of water (100 v/v)
A6
  • polyethylene glycol 6000 BCC 6.6 w/v -
  • sodium MES BCC 0.113 M pH 6.6
  • bis-tris chloride BCC 0.113 M pH 6.5
NA
24 μl of sodium MES BCC pH 5.2 (1 M)
88 μl of sodium MES BCC pH 7.2 (1 M)
113 μl of bis-tris chloride BCC pH 6.5 (1 M)
132 μl of polyethylene glycol 6000 BCC (50 w/v)
642 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 0.1 M -
  • glycerol BCC 22 v/v -
  • polyethylene glycol 4000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
275 μl of glycerol BCC (80 v/v)
28 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
240 μl of polyethylene glycol 4000 BCC (50 w/v)
356 μl of water (100 v/v)
A8
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 1500 BCC 7.7 w/v -
  • sodium chloride BCC 0.02 M -
  • sodium MES BCC 0.02 M pH 6.5
  • magnesium sulfate BCC 0.01 M -
pH 6.5
154 μl of polyethylene glycol 1500 BCC (50 w/v)
10 μl of magnesium sulfate BCC (1 M)
4 μl of sodium chloride BCC (5 M)
5 μl of sodium MES BCC pH 5.2 (1 M)
14 μl of sodium MES BCC pH 7.2 (1 M)
20 μl of calcium chloride BCC (1 M)
792 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 2.5 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
714 μl of ammonium sulfate BCC (3.5 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
235 μl of water (100 v/v)
A10
  • sodium HEPES BCC 0.067 M pH 7.5
  • trisodium citrate BCC 1.1 M -
pH 7.5
687 μl of trisodium citrate BCC (1.6 M)
33 μl of sodium HEPES BCC pH 8.5 (1 M)
33 μl of sodium HEPES BCC pH 6.5 (1 M)
245 μl of water (100 v/v)
A11
  • ammonium sulfate BCC 3 M -
  • dipotassium hydrogen phosphate BCC 0.15 M -
NA
75 μl of dipotassium hydrogen phosphate BCC (2 M)
857 μl of ammonium sulfate BCC (3.5 M)
67 μl of water (100 v/v)
A12
  • magnesium acetate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 5000 BCC 14 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.8
pH 5.8
700 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
14 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
40 μl of magnesium acetate BCC (2.5 M)
86 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
160 μl of water (100 v/v)
B1
  • polyethylene glycol 3350 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.02 M pH 5.6
pH 5.6
20 μl of sodium chloride BCC (5 M)
220 μl of polyethylene glycol 3350 BCC (50 w/v)
3 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
16 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
740 μl of water (100 v/v)
B2
  • polyethylene glycol 3350 BCC 5.5 w/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.02 M pH 5.6
pH 5.6
20 μl of sodium chloride BCC (5 M)
110 μl of polyethylene glycol 3350 BCC (50 w/v)
3 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
16 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
850 μl of water (100 v/v)
B3
  • barium chloride BCC 0.05 M -
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
50 μl of barium chloride BCC (1 M)
400 μl of polyethylene glycol 400 BCC (80 v/v)
40 μl of tris chloride BCC pH 7 (1 M)
59 μl of tris chloride BCC pH 9 (1 M)
50 μl of calcium chloride BCC (1 M)
400 μl of water (100 v/v)
B4
  • polyethylene glycol 4000 BCC 16 w/v -
  • sodium chloride BCC 0.05 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
10 μl of sodium chloride BCC (5 M)
80 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
320 μl of polyethylene glycol 4000 BCC (50 w/v)
20 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
570 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 4000 BCC 19 w/v -
  • tris chloride BCC 0.03 M pH 8.2
pH 8.2
100 μl of magnesium chloride BCC (1 M)
12 μl of tris chloride BCC pH 7 (1 M)
17 μl of tris chloride BCC pH 9 (1 M)
380 μl of polyethylene glycol 4000 BCC (50 w/v)
490 μl of water (100 v/v)
B6
  • polyethylene glycol 4000 BCC 13 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.025 M pH 7.5
pH 7.5
40 μl of sodium chloride BCC (5 M)
12 μl of sodium HEPES BCC pH 8.5 (1 M)
12 μl of sodium HEPES BCC pH 6.5 (1 M)
260 μl of polyethylene glycol 4000 BCC (50 w/v)
675 μl of water (100 v/v)
B7
  • polyethylene glycol 3350 BCC 11 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
220 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
680 μl of water (100 v/v)
B8
  • polyethylene glycol 4000 BCC 6.6 w/v -
  • potassium chloride BCC 0.02 M -
  • sodium chloride BCC 0.08 M -
  • sodium MES BCC 0.02 M pH 6.7
pH 6.7
16 μl of sodium chloride BCC (5 M)
3 μl of sodium MES BCC pH 5.2 (1 M)
6 μl of potassium chloride BCC (3 M)
16 μl of sodium MES BCC pH 7.2 (1 M)
132 μl of polyethylene glycol 4000 BCC (50 w/v)
825 μl of water (100 v/v)
B9
  • polyethylene glycol 4000 BCC 20 w/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
33 μl of potassium chloride BCC (3 M)
20 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
546 μl of water (100 v/v)
B10
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol 3350 BCC 6.6 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.7
pH 6.7
132 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of magnesium chloride BCC (1 M)
22 μl of sodium cacodylate BCC pH 5.3 (1 M)
77 μl of sodium cacodylate BCC pH 7.3 (1 M)
718 μl of water (100 v/v)
B11
  • potassium chloride BCC 0.2 M -
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 37 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
66 μl of potassium chloride BCC (3 M)
740 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
93 μl of water (100 v/v)
B12
  • polyethylene glycol 4000 BCC 5.5 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
110 μl of polyethylene glycol 4000 BCC (50 w/v)
790 μl of water (100 v/v)
C1
  • polyethylene glycol 4000 BCC 7.7 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
20 μl of sodium chloride BCC (5 M)
20 μl of tris chloride BCC pH 7 (1 M)
154 μl of polyethylene glycol 4000 BCC (50 w/v)
806 μl of water (100 v/v)
C2
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 22 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
275 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of magnesium chloride BCC (1 M)
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
525 μl of water (100 v/v)
C3
  • sodium chloride BCC 0.04 M -
  • tris chloride BCC 0.04 M pH 8
  • polyethylene glycol monomethyl ether 350 BCC 27 v/v -
pH 8
8 μl of sodium chloride BCC (5 M)
337 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
21 μl of tris chloride BCC pH 7 (1 M)
18 μl of tris chloride BCC pH 9 (1 M)
614 μl of water (100 v/v)
C4
  • magnesium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 22 v/v -
  • sodium chloride BCC 0.05 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
10 μl of sodium chloride BCC (5 M)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
275 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of magnesium chloride BCC (1 M)
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
595 μl of water (100 v/v)
C5
  • polyethylene glycol monomethyl ether 2000 BCC 8.8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
176 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
724 μl of water (100 v/v)
C6
  • ammonium acetate BCC 0.4 M -
  • polyethylene glycol monomethyl ether 2000 BCC 13 w/v -
NA
260 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
80 μl of ammonium acetate BCC (5 M)
660 μl of water (100 v/v)
C7
  • polyethylene glycol 2000 BCC 15 w/v -
  • bis-tris chloride BCC 0.02 M pH 7
pH 7
300 μl of polyethylene glycol 2000 BCC (50 w/v)
7 μl of bis-tris chloride BCC pH 6.5 (1 M)
12 μl of bis-tris chloride BCC pH 7.5 (1 M)
680 μl of water (100 v/v)
C8
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 1500 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
220 μl of polyethylene glycol 1500 BCC (50 w/v)
20 μl of sodium chloride BCC (5 M)
100 μl of magnesium chloride BCC (1 M)
16 μl of tris chloride BCC pH 7 (1 M)
3 μl of tris chloride BCC pH 9 (1 M)
640 μl of water (100 v/v)
C9
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 1500 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 8
pH 8
220 μl of polyethylene glycol 1500 BCC (50 w/v)
20 μl of sodium chloride BCC (5 M)
100 μl of magnesium chloride BCC (1 M)
81 μl of sodium HEPES BCC pH 8.5 (1 M)
18 μl of sodium HEPES BCC pH 6.5 (1 M)
560 μl of water (100 v/v)
C10
  • polyethylene glycol 3000 BCC 22 w/v -
  • potassium chloride BCC 0.2 M -
  • sodium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
66 μl of potassium chloride BCC (3 M)
440 μl of polyethylene glycol 3000 BCC (50 w/v)
200 μl of sodium acetate BCC (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
193 μl of water (100 v/v)
C11
  • jeffamine M-600 BCC 33 v/v -
  • sodium HEPES BCC 0.01 M pH 7
  • nickel(II) sulfate BCC 0.02 M -
pH 7
660 μl of jeffamine M-600 BCC pH 7 (50 v/v)
1 μl of sodium HEPES BCC pH 8.5 (1 M)
20 μl of nickel(II) sulfate BCC (1 M)
8 μl of sodium HEPES BCC pH 6.5 (1 M)
310 μl of water (100 v/v)
C12
  • polyethylene glycol 6000 BCC 13 w/v -
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
30 μl of sodium chloride BCC (5 M)
54 μl of tris chloride BCC pH 7 (1 M)
260 μl of polyethylene glycol 6000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
610 μl of water (100 v/v)
D1
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 53 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
662 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of calcium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
37 μl of water (100 v/v)
D2
  • magnesium acetate BCC 0.05 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
pH 5
350 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of magnesium acetate BCC (2.5 M)
17 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
32 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
580 μl of water (100 v/v)
D3
  • polyethylene glycol 4000 BCC 22 w/v -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
25 μl of sodium HEPES BCC pH 8.5 (1 M)
25 μl of sodium HEPES BCC pH 6.5 (1 M)
440 μl of polyethylene glycol 4000 BCC (50 w/v)
510 μl of water (100 v/v)
D4
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 44 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
550 μl of polyethylene glycol 400 BCC (80 v/v)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
200 μl of calcium chloride BCC (1 M)
150 μl of water (100 v/v)
D5
  • magnesium acetate BCC 0.05 M -
  • polyethylene glycol 400 BCC 24 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5.4
pH 5.4
300 μl of polyethylene glycol 400 BCC (80 v/v)
20 μl of magnesium acetate BCC (2.5 M)
6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
43 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
630 μl of water (100 v/v)
D6
  • calcium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol monomethyl ether 350 BCC 26 v/v -
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
325 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of calcium chloride BCC (1 M)
375 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 39 v/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
487 μl of polyethylene glycol 400 BCC (80 v/v)
33 μl of potassium chloride BCC (3 M)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
379 μl of water (100 v/v)
D8
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol 400 BCC 22 v/v -
  • glycine BCC 0.1 M pH 9
pH 9
275 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of magnesium chloride BCC (1 M)
30 μl of glycine BCC pH 8.5 (1 M)
69 μl of glycine BCC pH 10 (1 M)
575 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 0.1 M -
  • glycine BCC 0.1 M pH 3.8
  • triethylene glycol BCC 28 v/v -
pH 3.8
28 μl of ammonium sulfate BCC (3.5 M)
100 μl of glycine BCC pH 3.8 (1 M)
280 μl of triethylene glycol BCC (100 v/v)
591 μl of water (100 v/v)
D10
  • polyethylene glycol 3350 BCC 18 w/v -
  • sodium formate BCC 0.15 M -
  • sodium HEPES BCC 0.1 M pH 7.2
pH 7.2
360 μl of polyethylene glycol 3350 BCC (50 w/v)
29 μl of sodium HEPES BCC pH 8.5 (1 M)
37 μl of sodium formate BCC (4 M)
70 μl of sodium HEPES BCC pH 6.5 (1 M)
502 μl of water (100 v/v)
D11
  • polyethylene glycol 6000 BCC 8.8 w/v -
  • sodium acetate BCC 0.2 M -
NA
200 μl of sodium acetate BCC (1 M)
176 μl of polyethylene glycol 6000 BCC (50 w/v)
624 μl of water (100 v/v)
D12
  • polyethylene glycol 6000 BCC 18 w/v -
  • potassium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
66 μl of potassium chloride BCC (3 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
360 μl of polyethylene glycol 6000 BCC (50 w/v)
473 μl of water (100 v/v)
E1
  • polyethylene glycol 400 BCC 35 v/v -
  • tris chloride BCC 0.1 M pH 8
  • trisodium citrate BCC 0.22 M -
pH 8
437 μl of polyethylene glycol 400 BCC (80 v/v)
137 μl of trisodium citrate BCC (1.6 M)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
325 μl of water (100 v/v)
E2
  • polyethylene glycol 400 BCC 17 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
212 μl of polyethylene glycol 400 BCC (80 v/v)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
687 μl of water (100 v/v)
E3
  • lithium sulfate BCC 1 M -
  • tris chloride BCC 0.02 M pH 8.5
  • polyethylene glycol 8000 BCC 1.8 w/v -
pH 8.5
4 μl of tris chloride BCC pH 7 (1 M)
400 μl of lithium sulfate BCC (2.5 M)
36 μl of polyethylene glycol 8000 BCC (50 w/v)
15 μl of tris chloride BCC pH 9 (1 M)
544 μl of water (100 v/v)
E4
  • polyethylene glycol monomethyl ether 550 BCC 22 v/v -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
16 μl of tris chloride BCC pH 7 (1 M)
258 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
3 μl of tris chloride BCC pH 9 (1 M)
721 μl of water (100 v/v)
E5
  • polyethylene glycol 1000 BCC 33 w/v -
  • sodium chloride BCC 0.05 M -
  • glycine BCC 0.02 M pH 10
pH 10
10 μl of sodium chloride BCC (5 M)
0 μl of glycine BCC pH 8.5 (1 M)
19 μl of glycine BCC pH 10 (1 M)
660 μl of polyethylene glycol 1000 BCC (50 w/v)
310 μl of water (100 v/v)
E6
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 4000 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 4000 BCC (50 w/v)
200 μl of water (100 v/v)
E7
  • magnesium chloride BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 2000 BCC 31 w/v -
pH 6.5
200 μl of magnesium chloride BCC (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
620 μl of polyethylene glycol 2000 BCC (50 w/v)
80 μl of water (100 v/v)
E8
  • polyethylene glycol 3350 BCC 18 w/v -
  • sodium acetate-acetic acid BCC 0.64 M pH 4.6
pH 4.6
360 μl of polyethylene glycol 3350 BCC (50 w/v)
397 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
242 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
E9
  • cadmium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20 μl of sodium chloride BCC (5 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
54 μl of tris chloride BCC pH 7 (1 M)
100 μl of cadmium chloride BCC (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
367 μl of water (100 v/v)
E10
  • polyethylene glycol 2000 BCC 31 w/v -
  • sodium bicine BCC 0.1 M pH 8.9
pH 8.9
620 μl of polyethylene glycol 2000 BCC (50 w/v)
16 μl of sodium bicine BCC pH 7.4 (1 M)
83 μl of sodium bicine BCC pH 9.4 (1 M)
280 μl of water (100 v/v)
E11
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 400 BCC 35 v/v -
  • sodium sulfate BCC 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
437 μl of polyethylene glycol 400 BCC (80 v/v)
10 μl of tris chloride BCC pH 7 (1 M)
20 μl of lithium sulfate BCC (2.5 M)
39 μl of tris chloride BCC pH 9 (1 M)
41 μl of sodium sulfate BCC (1.2 M)
450 μl of water (100 v/v)
E12
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • glycine BCC 0.05 M pH 9.5
pH 9.5
20 μl of sodium chloride BCC (5 M)
4 μl of glycine BCC pH 8.5 (1 M)
45 μl of glycine BCC pH 10 (1 M)
412 μl of polyethylene glycol 300 BCC (80 v/v)
517 μl of water (100 v/v)
F1
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 2000 BCC 23 w/v -
pH 8
460 μl of polyethylene glycol 2000 BCC (50 w/v)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
300 μl of magnesium nitrate BCC (1 M)
140 μl of water (100 v/v)
F2
  • lithium sulfate BCC 0.12 M -
  • polyethylene glycol 300 BCC 20 v/v -
  • tris chloride BCC 0.02 M pH 7.5
  • trisodium citrate-citric acid BCC 0.1 M pH 5
NA
32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
16 μl of tris chloride BCC pH 7 (1 M)
48 μl of lithium sulfate BCC (2.5 M)
3 μl of tris chloride BCC pH 9 (1 M)
250 μl of polyethylene glycol 300 BCC (80 v/v)
68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
582 μl of water (100 v/v)
F3
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.12 M pH 9
pH 9
20 μl of sodium chloride BCC (5 M)
250 μl of polyethylene glycol 400 BCC (80 v/v)
120 μl of tris chloride BCC pH 9 (1 M)
610 μl of water (100 v/v)
F4
  • polyethylene glycol monomethyl ether 550 BCC 22 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
40 μl of sodium chloride BCC (5 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
258 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
601 μl of water (100 v/v)
F5
  • polyethylene glycol 400 BCC 21 v/v -
  • sodium acetate BCC 0.325 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20 μl of sodium chloride BCC (5 M)
262 μl of polyethylene glycol 400 BCC (80 v/v)
325 μl of sodium acetate BCC (1 M)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
292 μl of water (100 v/v)
F6
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.08 M pH 6.2
  • trisodium citrate BCC 0.02 M -
  • polyethylene glycol 2000 BCC 18 w/v -
pH 6.2
64 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
360 μl of polyethylene glycol 2000 BCC (50 w/v)
12 μl of trisodium citrate BCC (1.6 M)
16 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
547 μl of water (100 v/v)
F7
  • polyethylene glycol 4000 BCC 7.7 w/v -
  • tripotassium citrate BCC 0.03 M -
  • potassium nitrate BCC 0.02 M -
NA
30 μl of tripotassium citrate BCC (1 M)
20 μl of potassium nitrate BCC (1 M)
154 μl of polyethylene glycol 4000 BCC (50 w/v)
796 μl of water (100 v/v)
F8
  • magnesium chloride BCC 0.005 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
pH 8.5
20 μl of sodium chloride BCC (5 M)
5 μl of magnesium chloride BCC (1 M)
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
275 μl of water (100 v/v)
F9
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
412 μl of polyethylene glycol 400 BCC (80 v/v)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of calcium chloride BCC (1 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
287 μl of water (100 v/v)
F10
  • calcium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7
  • polyethylene glycol monomethyl ether 2000 BCC 13 w/v -
pH 7
260 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of tris chloride BCC pH 7 (1 M)
100 μl of calcium chloride BCC (1 M)
540 μl of water (100 v/v)
F11
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 200 BCC 33 v/v -
  • sodium chloride BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.02 M pH 4
pH 4
4 μl of sodium chloride BCC (5 M)
18 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
1 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
412 μl of polyethylene glycol 200 BCC (80 v/v)
506 μl of water (100 v/v)
F12
  • polyethylene glycol 400 BCC 22 v/v -
  • sodium chloride BCC 0.07 M -
  • trisodium citrate-citric acid BCC 0.5 M pH 4.5
pH 4.5
14 μl of sodium chloride BCC (5 M)
220 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
275 μl of polyethylene glycol 400 BCC (80 v/v)
280 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
211 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
329 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
513 μl of water (100 v/v)
G2
  • polyethylene glycol 400 BCC 55 v/v -
  • glycine BCC 0.05 M pH 9
pH 9
687 μl of polyethylene glycol 400 BCC (80 v/v)
15 μl of glycine BCC pH 8.5 (1 M)
34 μl of glycine BCC pH 10 (1 M)
262 μl of water (100 v/v)
G3
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
20 μl of sodium chloride BCC (5 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
100 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
367 μl of water (100 v/v)
G4
  • lithium sulfate BCC 0.1 M -
  • phosphate-citrate BCC 0.1 M pH 4
  • polyethylene glycol 1000 BCC 19 w/v -
pH 4
108 μl of phosphate-citrate BCC pH 9.3 (1 M)
92 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
40 μl of lithium sulfate BCC (2.5 M)
380 μl of polyethylene glycol 1000 BCC (50 w/v)
380 μl of water (100 v/v)
G5
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 33 v/v -
  • potassium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.025 M pH 4
pH 4
14 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of magnesium chloride BCC (1 M)
33 μl of potassium chloride BCC (3 M)
10 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
329 μl of water (100 v/v)
G6
  • sodium MES BCC 0.05 M pH 6.1
  • zinc acetate BCC 0.05 M -
  • polyethylene glycol 8000 BCC 11 w/v -
pH 6.1
27 μl of sodium MES BCC pH 5.2 (1 M)
50 μl of zinc acetate BCC (1 M)
22 μl of sodium MES BCC pH 7.2 (1 M)
220 μl of polyethylene glycol 8000 BCC (50 w/v)
680 μl of water (100 v/v)
G7
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 22 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
440 μl of polyethylene glycol 8000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
300 μl of magnesium nitrate BCC (1 M)
160 μl of water (100 v/v)
G8
  • ethylene glycol BCC 4 v/v -
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
20 μl of sodium chloride BCC (5 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
40 μl of ethylene glycol BCC (100 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
427 μl of water (100 v/v)
G9
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium chloride BCC 0.05 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
10 μl of sodium chloride BCC (5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325 μl of polyethylene glycol 400 BCC (80 v/v)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
565 μl of water (100 v/v)
G10
  • lithium sulfate BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • glycine BCC 0.1 M pH 9.3
pH 9.3
375 μl of polyethylene glycol 400 BCC (80 v/v)
15 μl of glycine BCC pH 8.5 (1 M)
85 μl of glycine BCC pH 10 (1 M)
40 μl of lithium sulfate BCC (2.5 M)
485 μl of water (100 v/v)
G11
  • polyethylene glycol 6000 BCC 22 w/v -
  • tripotassium citrate BCC 0.15 M -
  • trilithium citrate BCC 0.05 M -
  • sodium dihydrogen phosphate BCC 0.1 M -
NA
440 μl of polyethylene glycol 6000 BCC (50 w/v)
150 μl of tripotassium citrate BCC (1 M)
100 μl of sodium dihydrogen phosphate BCC (1 M)
50 μl of trilithium citrate BCC (1 M)
260 μl of water (100 v/v)
G12
  • polyethylene glycol 600 BCC 28 w/v -
  • sodium HEPES BCC 0.05 M pH 7.8
  • zinc sulfate BCC 0.001 M -
pH 7.8
35 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of zinc sulfate BCC (0.1 M)
560 μl of polyethylene glycol 600 BCC (50 w/v)
14 μl of sodium HEPES BCC pH 6.5 (1 M)
380 μl of water (100 v/v)
H1
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 7
pH 7
20 μl of sodium chloride BCC (5 M)
36 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
412 μl of polyethylene glycol 300 BCC (80 v/v)
64 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
467 μl of water (100 v/v)
H2
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium bicine BCC 0.05 M pH 9
pH 9
20 μl of sodium chloride BCC (5 M)
6 μl of sodium bicine BCC pH 7.4 (1 M)
43 μl of sodium bicine BCC pH 9.4 (1 M)
412 μl of polyethylene glycol 300 BCC (80 v/v)
517 μl of water (100 v/v)
H3
  • ethylene glycol BCC 6 v/v -
  • sodium cacodylate BCC 0.1 M pH 6
  • zinc acetate BCC 0.05 M -
  • polyethylene glycol 8000 BCC 6.6 w/v -
pH 6
50 μl of zinc acetate BCC (1 M)
60 μl of ethylene glycol BCC (100 v/v)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
132 μl of polyethylene glycol 8000 BCC (50 w/v)
658 μl of water (100 v/v)
H4
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
350 μl of polyethylene glycol 400 BCC (80 v/v)
80 μl of lithium sulfate BCC (2.5 M)
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
470 μl of water (100 v/v)
H5
  • polyethylene glycol 4000 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
20 μl of sodium chloride BCC (5 M)
84 μl of tris chloride BCC pH 7 (1 M)
15 μl of tris chloride BCC pH 9 (1 M)
220 μl of polyethylene glycol 4000 BCC (50 w/v)
660 μl of water (100 v/v)
H6
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 3000 BCC 7 w/v -
  • tricine BCC 0.1 M pH 7.4
pH 7.4
140 μl of polyethylene glycol 3000 BCC (50 w/v)
6 μl of tricine BCC pH 9.2 (1 M)
20 μl of lithium sulfate BCC (2.5 M)
93 μl of tricine BCC pH 7.2 (1 M)
740 μl of water (100 v/v)
H7
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 33 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
412 μl of polyethylene glycol 400 BCC (80 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of calcium chloride BCC (1 M)
287 μl of water (100 v/v)
H8
  • polyethylene glycol 4000 BCC 28 w/v -
  • sodium chloride BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
200 μl of sodium chloride BCC (5 M)
10 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
560 μl of polyethylene glycol 4000 BCC (50 w/v)
90 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
140 μl of water (100 v/v)
H9
  • polyethylene glycol 4000 BCC 11 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
220 μl of polyethylene glycol 4000 BCC (50 w/v)
680 μl of water (100 v/v)
H10
  • sodium HEPES BCC 0.08 M pH 7
  • zinc sulfate BCC 0.002 M -
  • jeffamine ED-2001 BCC 25 w/v -
pH 7
14 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
20 μl of zinc sulfate BCC (0.1 M)
65 μl of sodium HEPES BCC pH 6.5 (1 M)
400 μl of water (100 v/v)
H11
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
30 μl of magnesium chloride BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
495 μl of water (100 v/v)
H12
  • sodium chloride BCC 3 M -
  • bis-tris propane chloride BCC 0.1 M pH 7
pH 7
600 μl of sodium chloride BCC (5 M)
78 μl of bis-tris propane chloride BCC pH 6 (1 M)
22 μl of bis-tris propane chloride BCC pH 10 (1 M)
300 μl of water (100 v/v)