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Supplemental Information

Screen Description

BCC Core Screens / MemGold_C3
Created 2025-04-10.
Based on Molecular Dimensions MemGold screen, made up by C3, and modified to have only 80 stocks

Rigaku Recipe XML

μl
Stocks

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Barcodes

  • Z0002766 Lot 2 (1500.0 µl)
  • Z0002767 Lot 3 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 59
Internal diversity (Score mean dist)0.93
Internal diversity (Ion mean dist)0.87
Internal diversity (PEG mean dist)0.86
Internal diversity (Known pH mean dist)0.77
Internal diversity (Penalty mean dist)0.88

Screen Composition

Chemical classes bar chart
ingredient type MemGold_C3
Buffer 98
Cryoprotectant 2
Organic (non-volatile) 4
Polymer 89
Salt 120

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemGold_C3 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • ammonium sulfate BCC 2.2 M -
  • trisodium citrate-citric acid BCC 0.08 M pH 5.2
pH 5.2
628.57 μl of ammonium sulfate BCC (3.5 M)
57.47 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
22.53 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
291.43 μl of water (100 v/v)
A2
  • trisodium citrate BCC 1.2 M -
  • tris chloride BCC 0.01 M pH 8
pH 8
5.47 μl of tris chloride BCC pH 7 (1 M)
4.53 μl of tris chloride BCC pH 9 (1 M)
750.0 μl of trisodium citrate BCC (1.6 M)
240.0 μl of water (100 v/v)
A3
  • polyethylene glycol 4000 BCC 24 w/v -
  • tricine BCC 0.015 M pH 8.5
pH 8.5
480.0 μl of polyethylene glycol 4000 BCC (50 w/v)
4.16 μl of tricine BCC pH 7.2 (1 M)
10.84 μl of tricine BCC pH 9.2 (1 M)
505.0 μl of water (100 v/v)
A4
  • polyethylene glycol 4000 BCC 9.9 w/v -
  • sodium chloride BCC 0.36 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.015 M pH 7
pH 7
198.0 μl of polyethylene glycol 4000 BCC (50 w/v)
72.0 μl of sodium chloride BCC (5 M)
5.67 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
9.33 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
715.0 μl of water (100 v/v)
A5
  • polyethylene glycol 4000 BCC 27.5 w/v -
  • sodium chloride BCC 0.3 M -
  • tris chloride BCC 0.01 M pH 8
pH 8
550.0 μl of polyethylene glycol 4000 BCC (50 w/v)
60.0 μl of sodium chloride BCC (5 M)
5.47 μl of tris chloride BCC pH 7 (1 M)
4.53 μl of tris chloride BCC pH 9 (1 M)
380.0 μl of water (100 v/v)
A6
  • polyethylene glycol 6000 BCC 6.6 w/v -
  • sodium MES BCC 0.113 M pH 6.6
  • bis-tris chloride BCC 0.113 M pH 6.5
NA
113.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
132.0 μl of polyethylene glycol 6000 BCC (50 w/v)
24.9 μl of sodium MES BCC pH 5.2 (1 M)
88.1 μl of sodium MES BCC pH 7.2 (1 M)
642.0 μl of water (100 v/v)
A7
  • glycerol BCC 22 v/v -
  • ammonium sulfate BCC 0.1 M -
  • polyethylene glycol 4000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
28.57 μl of ammonium sulfate BCC (3.5 M)
275.0 μl of glycerol BCC (80 v/v)
240.0 μl of polyethylene glycol 4000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
356.43 μl of water (100 v/v)
A8
  • polyethylene glycol 1500 BCC 7.7 w/v -
  • calcium chloride BCC 0.02 M -
  • sodium chloride BCC 0.02 M -
  • magnesium sulfate BCC 0.01 M -
  • sodium MES BCC 0.02 M pH 6.5
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
10.0 μl of magnesium sulfate BCC (1 M)
154.0 μl of polyethylene glycol 1500 BCC (50 w/v)
4.0 μl of sodium chloride BCC (5 M)
5.55 μl of sodium MES BCC pH 5.2 (1 M)
14.45 μl of sodium MES BCC pH 7.2 (1 M)
792.0 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 2.5 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
714.29 μl of ammonium sulfate BCC (3.5 M)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
235.71 μl of water (100 v/v)
A10
  • trisodium citrate BCC 1.1 M -
  • sodium HEPES BCC 0.067 M pH 7.5
pH 7.5
33.5 μl of sodium HEPES BCC pH 6.5 (1 M)
33.5 μl of sodium HEPES BCC pH 8.5 (1 M)
687.5 μl of trisodium citrate BCC (1.6 M)
245.5 μl of water (100 v/v)
A11
  • ammonium sulfate BCC 3 M -
  • dipotassium hydrogen phosphate BCC 0.15 M -
NA
857.14 μl of ammonium sulfate BCC (3.5 M)
75.0 μl of dipotassium hydrogen phosphate BCC (2 M)
67.86 μl of water (100 v/v)
A12
  • polyethylene glycol monomethyl ether 5000 BCC 14 w/v -
  • magnesium acetate BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.8
pH 5.8
40.0 μl of magnesium acetate BCC (2.5 M)
700.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
85.64 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
14.36 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
160.0 μl of water (100 v/v)
B1
  • polyethylene glycol 3350 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.02 M pH 5.6
pH 5.6
220.0 μl of polyethylene glycol 3350 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
16.29 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
3.71 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
740.0 μl of water (100 v/v)
B2
  • polyethylene glycol 3350 BCC 5.5 w/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.02 M pH 5.6
pH 5.6
110.0 μl of polyethylene glycol 3350 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
16.29 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
3.71 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
850.0 μl of water (100 v/v)
B3
  • polyethylene glycol 400 BCC 32 v/v -
  • barium chloride BCC 0.05 M -
  • calcium chloride BCC 0.05 M -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
50.0 μl of barium chloride BCC (1 M)
50.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.62 μl of tris chloride BCC pH 7 (1 M)
59.38 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
B4
  • polyethylene glycol 4000 BCC 16 w/v -
  • sodium chloride BCC 0.05 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
320.0 μl of polyethylene glycol 4000 BCC (50 w/v)
10.0 μl of sodium chloride BCC (5 M)
79.74 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
20.26 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
570.0 μl of water (100 v/v)
B5
  • polyethylene glycol 4000 BCC 19 w/v -
  • magnesium chloride BCC 0.1 M -
  • tris chloride BCC 0.03 M pH 8.2
pH 8.2
100.0 μl of magnesium chloride BCC (1 M)
380.0 μl of polyethylene glycol 4000 BCC (50 w/v)
12.18 μl of tris chloride BCC pH 7 (1 M)
17.82 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)
B6
  • polyethylene glycol 4000 BCC 13 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.025 M pH 7.5
pH 7.5
260.0 μl of polyethylene glycol 4000 BCC (50 w/v)
40.0 μl of sodium chloride BCC (5 M)
12.5 μl of sodium HEPES BCC pH 6.5 (1 M)
12.5 μl of sodium HEPES BCC pH 8.5 (1 M)
675.0 μl of water (100 v/v)
B7
  • polyethylene glycol 3350 BCC 11 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
220.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
680.0 μl of water (100 v/v)
B8
  • polyethylene glycol 4000 BCC 6.6 w/v -
  • potassium chloride BCC 0.02 M -
  • sodium chloride BCC 0.08 M -
  • sodium MES BCC 0.02 M pH 6.7
pH 6.7
132.0 μl of polyethylene glycol 4000 BCC (50 w/v)
6.67 μl of potassium chloride BCC (3 M)
16.0 μl of sodium chloride BCC (5 M)
3.38 μl of sodium MES BCC pH 5.2 (1 M)
16.62 μl of sodium MES BCC pH 7.2 (1 M)
825.33 μl of water (100 v/v)
B9
  • polyethylene glycol 4000 BCC 20 w/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
33.33 μl of potassium chloride BCC (3 M)
20.0 μl of tris chloride BCC pH 7 (1 M)
546.67 μl of water (100 v/v)
B10
  • polyethylene glycol 3350 BCC 6.6 w/v -
  • magnesium chloride BCC 0.05 M -
  • sodium cacodylate BCC 0.1 M pH 6.7
pH 6.7
50.0 μl of magnesium chloride BCC (1 M)
132.0 μl of polyethylene glycol 3350 BCC (50 w/v)
22.68 μl of sodium cacodylate BCC pH 5.3 (1 M)
77.32 μl of sodium cacodylate BCC pH 7.3 (1 M)
718.0 μl of water (100 v/v)
B11
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 37 w/v -
  • potassium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
740.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
66.67 μl of potassium chloride BCC (3 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
93.33 μl of water (100 v/v)
B12
  • polyethylene glycol 4000 BCC 5.5 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
110.0 μl of polyethylene glycol 4000 BCC (50 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
790.0 μl of water (100 v/v)
C1
  • polyethylene glycol 4000 BCC 7.7 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
154.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
20.0 μl of tris chloride BCC pH 7 (1 M)
806.0 μl of water (100 v/v)
C2
  • polyethylene glycol 400 BCC 22 v/v -
  • magnesium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of magnesium chloride BCC (1 M)
275.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.39 μl of tris chloride BCC pH 7 (1 M)
15.61 μl of tris chloride BCC pH 9 (1 M)
525.0 μl of water (100 v/v)
C3
  • polyethylene glycol monomethyl ether 350 BCC 27 v/v -
  • sodium chloride BCC 0.04 M -
  • tris chloride BCC 0.04 M pH 8
pH 8
337.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
8.0 μl of sodium chloride BCC (5 M)
21.88 μl of tris chloride BCC pH 7 (1 M)
18.12 μl of tris chloride BCC pH 9 (1 M)
614.5 μl of water (100 v/v)
C4
  • polyethylene glycol 400 BCC 22 v/v -
  • magnesium chloride BCC 0.02 M -
  • sodium chloride BCC 0.05 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
20.0 μl of magnesium chloride BCC (1 M)
275.0 μl of polyethylene glycol 400 BCC (80 v/v)
10.0 μl of sodium chloride BCC (5 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
595.0 μl of water (100 v/v)
C5
  • polyethylene glycol monomethyl ether 2000 BCC 8.8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
176.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
91.39 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.61 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
724.0 μl of water (100 v/v)
C6
  • polyethylene glycol monomethyl ether 2000 BCC 13 w/v -
  • ammonium acetate BCC 0.4 M -
NA
80.0 μl of ammonium acetate BCC (5 M)
260.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
660.0 μl of water (100 v/v)
C7
  • polyethylene glycol 2000 BCC 15 w/v -
  • bis-tris chloride BCC 0.02 M pH 7
pH 7
7.13 μl of bis-tris chloride BCC pH 6.5 (1 M)
12.87 μl of bis-tris chloride BCC pH 7.5 (1 M)
300.0 μl of polyethylene glycol 2000 BCC (50 w/v)
680.0 μl of water (100 v/v)
C8
  • polyethylene glycol 1500 BCC 11 w/v -
  • magnesium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
100.0 μl of magnesium chloride BCC (1 M)
220.0 μl of polyethylene glycol 1500 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
16.88 μl of tris chloride BCC pH 7 (1 M)
3.12 μl of tris chloride BCC pH 9 (1 M)
640.0 μl of water (100 v/v)
C9
  • polyethylene glycol 1500 BCC 11 w/v -
  • magnesium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 8
pH 8
100.0 μl of magnesium chloride BCC (1 M)
220.0 μl of polyethylene glycol 1500 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
18.25 μl of sodium HEPES BCC pH 6.5 (1 M)
81.75 μl of sodium HEPES BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
C10
  • polyethylene glycol 3000 BCC 22 w/v -
  • potassium chloride BCC 0.2 M -
  • sodium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
440.0 μl of polyethylene glycol 3000 BCC (50 w/v)
66.67 μl of potassium chloride BCC (3 M)
200.0 μl of sodium acetate BCC (1 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
193.33 μl of water (100 v/v)
C11
  • jeffamine M-600 BCC 33 v/v -
  • nickel(II) sulfate BCC 0.02 M -
  • sodium HEPES BCC 0.01 M pH 7
pH 7
660.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
20.0 μl of nickel(II) sulfate BCC (1 M)
8.18 μl of sodium HEPES BCC pH 6.5 (1 M)
1.82 μl of sodium HEPES BCC pH 8.5 (1 M)
310.0 μl of water (100 v/v)
C12
  • polyethylene glycol 6000 BCC 13 w/v -
  • sodium chloride BCC 0.15 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
260.0 μl of polyethylene glycol 6000 BCC (50 w/v)
30.0 μl of sodium chloride BCC (5 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
610.0 μl of water (100 v/v)
D1
  • polyethylene glycol 400 BCC 53 v/v -
  • calcium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of calcium chloride BCC (1 M)
662.5 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
37.5 μl of water (100 v/v)
D2
  • polyethylene glycol 400 BCC 28 v/v -
  • magnesium acetate BCC 0.05 M -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
pH 5
20.0 μl of magnesium acetate BCC (2.5 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
32.77 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.23 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
580.0 μl of water (100 v/v)
D3
  • polyethylene glycol 4000 BCC 22 w/v -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
440.0 μl of polyethylene glycol 4000 BCC (50 w/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
510.0 μl of water (100 v/v)
D4
  • polyethylene glycol 400 BCC 44 v/v -
  • calcium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of calcium chloride BCC (1 M)
550.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
150.0 μl of water (100 v/v)
D5
  • polyethylene glycol 400 BCC 24 v/v -
  • magnesium acetate BCC 0.05 M -
  • sodium acetate-acetic acid BCC 0.05 M pH 5.4
pH 5.4
20.0 μl of magnesium acetate BCC (2.5 M)
300.0 μl of polyethylene glycol 400 BCC (80 v/v)
43.71 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
6.29 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
630.0 μl of water (100 v/v)
D6
  • polyethylene glycol monomethyl ether 350 BCC 26 v/v -
  • calcium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of calcium chloride BCC (1 M)
325.0 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
375.0 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 39 v/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
487.5 μl of polyethylene glycol 400 BCC (80 v/v)
33.33 μl of potassium chloride BCC (3 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
379.17 μl of water (100 v/v)
D8
  • polyethylene glycol 400 BCC 22 v/v -
  • magnesium chloride BCC 0.05 M -
  • glycine BCC 0.1 M pH 9
pH 9
84.39 μl of glycine BCC pH 8.5 (1 M)
15.61 μl of glycine BCC pH 10.5 (1 M)
50.0 μl of magnesium chloride BCC (1 M)
275.0 μl of polyethylene glycol 400 BCC (80 v/v)
575.0 μl of water (100 v/v)
D9
  • triethylene glycol BCC 28 v/v -
  • ammonium sulfate BCC 0.1 M -
  • glycine BCC 0.1 M pH 3.8
pH 3.8
28.57 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of glycine BCC pH 3.8 (1 M)
280.0 μl of triethylene glycol BCC (100 v/v)
591.43 μl of water (100 v/v)
D10
  • polyethylene glycol 3350 BCC 18 w/v -
  • sodium formate BCC 0.15 M -
  • sodium HEPES BCC 0.1 M pH 7.2
pH 7.2
360.0 μl of polyethylene glycol 3350 BCC (50 w/v)
37.5 μl of sodium formate BCC (4 M)
70.31 μl of sodium HEPES BCC pH 6.5 (1 M)
29.69 μl of sodium HEPES BCC pH 8.5 (1 M)
502.5 μl of water (100 v/v)
D11
  • polyethylene glycol 6000 BCC 8.8 w/v -
  • sodium acetate BCC 0.2 M -
NA
176.0 μl of polyethylene glycol 6000 BCC (50 w/v)
200.0 μl of sodium acetate BCC (1 M)
624.0 μl of water (100 v/v)
D12
  • polyethylene glycol 6000 BCC 18 w/v -
  • potassium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
360.0 μl of polyethylene glycol 6000 BCC (50 w/v)
66.67 μl of potassium chloride BCC (3 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
473.33 μl of water (100 v/v)
E1
  • polyethylene glycol 400 BCC 35 v/v -
  • trisodium citrate BCC 0.22 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
137.5 μl of trisodium citrate BCC (1.6 M)
325.0 μl of water (100 v/v)
E2
  • polyethylene glycol 400 BCC 17 v/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
212.5 μl of polyethylene glycol 400 BCC (80 v/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
687.5 μl of water (100 v/v)
E3
  • polyethylene glycol 8000 BCC 1.8 w/v -
  • lithium sulfate BCC 1 M -
  • tris chloride BCC 0.02 M pH 8.5
pH 8.5
400.0 μl of lithium sulfate BCC (2.5 M)
36.0 μl of polyethylene glycol 8000 BCC (50 w/v)
4.25 μl of tris chloride BCC pH 7 (1 M)
15.75 μl of tris chloride BCC pH 9 (1 M)
544.0 μl of water (100 v/v)
E4
  • polyethylene glycol monomethyl ether 550 BCC 22 v/v -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
258.82 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
16.88 μl of tris chloride BCC pH 7 (1 M)
3.12 μl of tris chloride BCC pH 9 (1 M)
721.18 μl of water (100 v/v)
E5
  • polyethylene glycol 1000 BCC 33 w/v -
  • sodium chloride BCC 0.05 M -
  • glycine BCC 0.02 M pH 10
pH 10
4.25 μl of glycine BCC pH 8.5 (1 M)
15.75 μl of glycine BCC pH 10.5 (1 M)
660.0 μl of polyethylene glycol 1000 BCC (50 w/v)
10.0 μl of sodium chloride BCC (5 M)
310.0 μl of water (100 v/v)
E6
  • polyethylene glycol 4000 BCC 25 w/v -
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
200.0 μl of water (100 v/v)
E7
  • polyethylene glycol 2000 BCC 31 w/v -
  • magnesium chloride BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of magnesium chloride BCC (1 M)
620.0 μl of polyethylene glycol 2000 BCC (50 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
80.0 μl of water (100 v/v)
E8
  • polyethylene glycol 3350 BCC 18 w/v -
  • sodium acetate-acetic acid BCC 0.64 M pH 4.6
pH 4.6
360.0 μl of polyethylene glycol 3350 BCC (50 w/v)
242.75 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
397.25 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
E9
  • polyethylene glycol 400 BCC 33 v/v -
  • cadmium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of cadmium chloride BCC (1 M)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
367.5 μl of water (100 v/v)
E10
  • polyethylene glycol 2000 BCC 31 w/v -
  • sodium bicine BCC 0.1 M pH 8.9
pH 8.9
620.0 μl of polyethylene glycol 2000 BCC (50 w/v)
16.89 μl of sodium bicine BCC pH 7.4 (1 M)
83.11 μl of sodium bicine BCC pH 9.4 (1 M)
280.0 μl of water (100 v/v)
E11
  • polyethylene glycol 400 BCC 35 v/v -
  • lithium sulfate BCC 0.05 M -
  • sodium sulfate BCC 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
20.0 μl of lithium sulfate BCC (2.5 M)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
41.67 μl of sodium sulfate BCC (1.2 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
450.83 μl of water (100 v/v)
E12
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • glycine BCC 0.05 M pH 9.5
pH 9.5
27.34 μl of glycine BCC pH 8.5 (1 M)
22.66 μl of glycine BCC pH 10.5 (1 M)
412.5 μl of polyethylene glycol 300 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
517.5 μl of water (100 v/v)
F1
  • polyethylene glycol 2000 BCC 23 w/v -
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of magnesium nitrate BCC (1 M)
460.0 μl of polyethylene glycol 2000 BCC (50 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
140.0 μl of water (100 v/v)
F2
  • polyethylene glycol 300 BCC 20 v/v -
  • lithium sulfate BCC 0.12 M -
  • tris chloride BCC 0.02 M pH 7.5
  • trisodium citrate-citric acid BCC 0.1 M pH 5
NA
48.0 μl of lithium sulfate BCC (2.5 M)
250.0 μl of polyethylene glycol 300 BCC (80 v/v)
16.88 μl of tris chloride BCC pH 7 (1 M)
3.12 μl of tris chloride BCC pH 9 (1 M)
67.33 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.67 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
582.0 μl of water (100 v/v)
F3
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.12 M pH 9
pH 9
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
120.0 μl of tris chloride BCC pH 9 (1 M)
610.0 μl of water (100 v/v)
F4
  • polyethylene glycol monomethyl ether 550 BCC 22 v/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
258.82 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
40.0 μl of sodium chloride BCC (5 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
601.18 μl of water (100 v/v)
F5
  • polyethylene glycol 400 BCC 21 v/v -
  • sodium acetate BCC 0.325 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
262.5 μl of polyethylene glycol 400 BCC (80 v/v)
325.0 μl of sodium acetate BCC (1 M)
20.0 μl of sodium chloride BCC (5 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
292.5 μl of water (100 v/v)
F6
  • polyethylene glycol 2000 BCC 18 w/v -
  • trisodium citrate BCC 0.02 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.08 M pH 6.2
pH 6.2
360.0 μl of polyethylene glycol 2000 BCC (50 w/v)
63.8 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
16.2 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
12.5 μl of trisodium citrate BCC (1.6 M)
547.5 μl of water (100 v/v)
F7
  • polyethylene glycol 4000 BCC 7.7 w/v -
  • tripotassium citrate BCC 0.03 M -
  • potassium nitrate BCC 0.02 M -
NA
154.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of potassium nitrate BCC (1 M)
30.0 μl of tripotassium citrate BCC (1 M)
796.0 μl of water (100 v/v)
F8
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
  • magnesium chloride BCC 0.005 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
5.0 μl of magnesium chloride BCC (1 M)
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
275.0 μl of water (100 v/v)
F9
  • polyethylene glycol 400 BCC 33 v/v -
  • calcium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
200.0 μl of calcium chloride BCC (1 M)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
287.5 μl of water (100 v/v)
F10
  • polyethylene glycol monomethyl ether 2000 BCC 13 w/v -
  • calcium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
100.0 μl of calcium chloride BCC (1 M)
260.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
540.0 μl of water (100 v/v)
F11
  • polyethylene glycol 200 BCC 33 v/v -
  • ammonium sulfate BCC 0.2 M -
  • sodium chloride BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.02 M pH 4
pH 4
57.14 μl of ammonium sulfate BCC (3.5 M)
412.5 μl of polyethylene glycol 200 BCC (80 v/v)
1.2 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
18.8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
4.0 μl of sodium chloride BCC (5 M)
506.36 μl of water (100 v/v)
F12
  • polyethylene glycol 400 BCC 22 v/v -
  • sodium chloride BCC 0.07 M -
  • trisodium citrate-citric acid BCC 0.5 M pH 4.5
pH 4.5
275.0 μl of polyethylene glycol 400 BCC (80 v/v)
14.0 μl of sodium chloride BCC (5 M)
275.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
225.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
211.0 μl of water (100 v/v)
G1
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
  • ammonium sulfate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
57.14 μl of ammonium sulfate BCC (3.5 M)
329.41 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
37.93 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.07 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
513.45 μl of water (100 v/v)
G2
  • polyethylene glycol 400 BCC 55 v/v -
  • glycine BCC 0.05 M pH 9
pH 9
42.19 μl of glycine BCC pH 8.5 (1 M)
7.81 μl of glycine BCC pH 10.5 (1 M)
687.5 μl of polyethylene glycol 400 BCC (80 v/v)
262.5 μl of water (100 v/v)
G3
  • polyethylene glycol 400 BCC 33 v/v -
  • magnesium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of magnesium chloride BCC (1 M)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
367.5 μl of water (100 v/v)
G4
  • polyethylene glycol 1000 BCC 19 w/v -
  • lithium sulfate BCC 0.1 M -
  • phosphate-citrate BCC 0.1 M pH 4
pH 4
40.0 μl of lithium sulfate BCC (2.5 M)
92.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
108.0 μl of phosphate-citrate BCC pH 9.3 (1 M)
380.0 μl of polyethylene glycol 1000 BCC (50 w/v)
380.0 μl of water (100 v/v)
G5
  • polyethylene glycol 400 BCC 33 v/v -
  • magnesium chloride BCC 0.2 M -
  • potassium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.025 M pH 4
pH 4
200.0 μl of magnesium chloride BCC (1 M)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
33.33 μl of potassium chloride BCC (3 M)
10.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
14.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
329.17 μl of water (100 v/v)
G6
  • polyethylene glycol 8000 BCC 11 w/v -
  • zinc acetate BCC 0.05 M -
  • sodium MES BCC 0.05 M pH 6.1
pH 6.1
220.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.34 μl of sodium MES BCC pH 5.2 (1 M)
22.66 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of zinc acetate BCC (1 M)
680.0 μl of water (100 v/v)
G7
  • polyethylene glycol 8000 BCC 22 w/v -
  • magnesium nitrate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of magnesium nitrate BCC (1 M)
440.0 μl of polyethylene glycol 8000 BCC (50 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
160.0 μl of water (100 v/v)
G8
  • polyethylene glycol 400 BCC 33 v/v -
  • ethylene glycol BCC 4 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
40.0 μl of ethylene glycol BCC (100 v/v)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
427.5 μl of water (100 v/v)
G9
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium chloride BCC 0.05 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
10.0 μl of sodium chloride BCC (5 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
565.0 μl of water (100 v/v)
G10
  • polyethylene glycol 400 BCC 30 v/v -
  • lithium sulfate BCC 0.1 M -
  • glycine BCC 0.1 M pH 9.3
pH 9.3
68.05 μl of glycine BCC pH 8.5 (1 M)
31.95 μl of glycine BCC pH 10.5 (1 M)
40.0 μl of lithium sulfate BCC (2.5 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
485.0 μl of water (100 v/v)
G11
  • polyethylene glycol 6000 BCC 22 w/v -
  • tripotassium citrate BCC 0.15 M -
  • trilithium citrate BCC 0.05 M -
  • sodium dihydrogen phosphate BCC 0.1 M -
NA
440.0 μl of polyethylene glycol 6000 BCC (50 w/v)
100.0 μl of sodium dihydrogen phosphate BCC (1 M)
50.0 μl of trilithium citrate BCC (1 M)
150.0 μl of tripotassium citrate BCC (1 M)
260.0 μl of water (100 v/v)
G12
  • polyethylene glycol 600 BCC 28 w/v -
  • zinc sulfate BCC 0.001 M -
  • sodium HEPES BCC 0.05 M pH 7.8
pH 7.8
560.0 μl of polyethylene glycol 600 BCC (50 w/v)
14.85 μl of sodium HEPES BCC pH 6.5 (1 M)
35.15 μl of sodium HEPES BCC pH 8.5 (1 M)
10.0 μl of zinc sulfate BCC (0.1 M)
380.0 μl of water (100 v/v)
H1
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 7
pH 7
412.5 μl of polyethylene glycol 300 BCC (80 v/v)
20.0 μl of sodium chloride BCC (5 M)
37.82 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
62.18 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
467.5 μl of water (100 v/v)
H2
  • polyethylene glycol 300 BCC 33 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium bicine BCC 0.05 M pH 9
pH 9
412.5 μl of polyethylene glycol 300 BCC (80 v/v)
6.18 μl of sodium bicine BCC pH 7.4 (1 M)
43.82 μl of sodium bicine BCC pH 9.4 (1 M)
20.0 μl of sodium chloride BCC (5 M)
517.5 μl of water (100 v/v)
H3
  • polyethylene glycol 8000 BCC 6.6 w/v -
  • ethylene glycol BCC 6 v/v -
  • zinc acetate BCC 0.05 M -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
60.0 μl of ethylene glycol BCC (100 v/v)
132.0 μl of polyethylene glycol 8000 BCC (50 w/v)
69.11 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.89 μl of sodium cacodylate BCC pH 7.3 (1 M)
50.0 μl of zinc acetate BCC (1 M)
658.0 μl of water (100 v/v)
H4
  • polyethylene glycol 400 BCC 28 v/v -
  • lithium sulfate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
80.0 μl of lithium sulfate BCC (2.5 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
29.83 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70.17 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
470.0 μl of water (100 v/v)
H5
  • polyethylene glycol 4000 BCC 11 w/v -
  • sodium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
220.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of sodium chloride BCC (5 M)
84.39 μl of tris chloride BCC pH 7 (1 M)
15.61 μl of tris chloride BCC pH 9 (1 M)
660.0 μl of water (100 v/v)
H6
  • polyethylene glycol 3000 BCC 7 w/v -
  • lithium sulfate BCC 0.05 M -
  • tricine BCC 0.1 M pH 7.4
pH 7.4
20.0 μl of lithium sulfate BCC (2.5 M)
140.0 μl of polyethylene glycol 3000 BCC (50 w/v)
93.87 μl of tricine BCC pH 7.2 (1 M)
6.13 μl of tricine BCC pH 9.2 (1 M)
740.0 μl of water (100 v/v)
H7
  • polyethylene glycol 400 BCC 33 v/v -
  • calcium chloride BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of calcium chloride BCC (1 M)
412.5 μl of polyethylene glycol 400 BCC (80 v/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
287.5 μl of water (100 v/v)
H8
  • polyethylene glycol 4000 BCC 28 w/v -
  • sodium chloride BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
560.0 μl of polyethylene glycol 4000 BCC (50 w/v)
200.0 μl of sodium chloride BCC (5 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
140.0 μl of water (100 v/v)
H9
  • polyethylene glycol 4000 BCC 11 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
220.0 μl of polyethylene glycol 4000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
680.0 μl of water (100 v/v)
H10
  • jeffamine ED-2001 BCC 25 w/v -
  • zinc sulfate BCC 0.002 M -
  • sodium HEPES BCC 0.08 M pH 7
pH 7
500.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
65.4 μl of sodium HEPES BCC pH 6.5 (1 M)
14.6 μl of sodium HEPES BCC pH 8.5 (1 M)
20.0 μl of zinc sulfate BCC (0.1 M)
400.0 μl of water (100 v/v)
H11
  • polyethylene glycol 400 BCC 30 v/v -
  • magnesium chloride BCC 0.03 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
30.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
495.0 μl of water (100 v/v)
H12
  • sodium chloride BCC 3 M -
  • bis-tris propane chloride BCC 0.1 M pH 7
pH 7
77.2 μl of bis-tris propane chloride BCC pH 6 (1 M)
22.8 μl of bis-tris propane chloride BCC pH 10 (1 M)
600.0 μl of sodium chloride BCC (5 M)
300.0 μl of water (100 v/v)