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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
MemGold_C3
BCC Core Screens / MemGold_C3
Created 2025-04-10.
Based on Molecular Dimensions MemGold screen, made up by C3, and modified to have only 80 stocks
Export
MemGold_C3
Barcodes
MemGold_C3
Screen Structure
MemGold_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 59 |
Internal diversity (score mean dist) | 0.927 |
Screen Composition
ingredient type | MemGold_C3 |
---|---|
Buffer | 98 |
Cryoprotectant | 2 |
Organic (non-volatile) | 4 |
Polymer | 89 |
Salt | 120 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.2 | 628.6 μl of ammonium sulfate BCC (3.5 M) 57.6 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 22.4 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 291.4 μl of water (100 v/v) |
A2 |
| pH 8 | 5.5 μl of tris chloride BCC pH 7 (1 M) 4.5 μl of tris chloride BCC pH 9 (1 M) 750.0 μl of trisodium citrate BCC (1.6 M) 240.0 μl of water (100 v/v) |
A3 |
| pH 8.5 | 480.0 μl of polyethylene glycol 4000 BCC (50 w/v) 4.2 μl of tricine BCC pH 7.2 (1 M) 10.8 μl of tricine BCC pH 9.2 (1 M) 505.0 μl of water (100 v/v) |
A4 |
| pH 7 | 198.0 μl of polyethylene glycol 4000 BCC (50 w/v) 72.0 μl of sodium chloride BCC (5 M) 5.4 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 9.6 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 715.0 μl of water (100 v/v) |
A5 |
| pH 8 | 550.0 μl of polyethylene glycol 4000 BCC (50 w/v) 60.0 μl of sodium chloride BCC (5 M) 5.5 μl of tris chloride BCC pH 7 (1 M) 4.5 μl of tris chloride BCC pH 9 (1 M) 380.0 μl of water (100 v/v) |
A6 |
| NA | 113.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 132.0 μl of polyethylene glycol 6000 BCC (50 w/v) 24.9 μl of sodium MES BCC pH 5.2 (1 M) 88.1 μl of sodium MES BCC pH 7.2 (1 M) 642.0 μl of water (100 v/v) |
A7 |
| pH 7.5 | 28.6 μl of ammonium sulfate BCC (3.5 M) 275.0 μl of glycerol BCC (80 v/v) 240.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 356.4 μl of water (100 v/v) |
A8 |
| pH 6.5 | 20.0 μl of calcium chloride BCC (1 M) 10.0 μl of magnesium sulfate BCC (1 M) 154.0 μl of polyethylene glycol 1500 BCC (50 w/v) 5.6 μl of sodium MES BCC pH 5.2 (1 M) 14.4 μl of sodium MES BCC pH 7.2 (1 M) 4.0 μl of sodium chloride BCC (5 M) 792.0 μl of water (100 v/v) |
A9 |
| pH 7.5 | 714.3 μl of ammonium sulfate BCC (3.5 M) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 235.7 μl of water (100 v/v) |
A10 |
| pH 7.5 | 33.5 μl of sodium HEPES BCC pH 6.5 (1 M) 33.5 μl of sodium HEPES BCC pH 8.5 (1 M) 687.5 μl of trisodium citrate BCC (1.6 M) 245.5 μl of water (100 v/v) |
A11 |
| NA | 857.1 μl of ammonium sulfate BCC (3.5 M) 75.0 μl of dipotassium hydrogen phosphate BCC (2 M) 67.9 μl of water (100 v/v) |
A12 |
| pH 5.8 | 40.0 μl of magnesium acetate BCC (2.5 M) 700.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 86.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 14.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 160.0 μl of water (100 v/v) |
B1 |
| pH 5.6 | 220.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 16.4 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 3.6 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 740.0 μl of water (100 v/v) |
B2 |
| pH 5.6 | 110.0 μl of polyethylene glycol 3350 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 16.4 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 3.6 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 850.0 μl of water (100 v/v) |
B3 |
| pH 8.2 | 50.0 μl of barium chloride BCC (1 M) 50.0 μl of calcium chloride BCC (1 M) 400.0 μl of polyethylene glycol 400 BCC (80 v/v) 40.6 μl of tris chloride BCC pH 7 (1 M) 59.4 μl of tris chloride BCC pH 9 (1 M) 400.0 μl of water (100 v/v) |
B4 |
| pH 6.2 | 320.0 μl of polyethylene glycol 4000 BCC (50 w/v) 10.0 μl of sodium chloride BCC (5 M) 80.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 20.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 570.0 μl of water (100 v/v) |
B5 |
| pH 8.2 | 100.0 μl of magnesium chloride BCC (1 M) 380.0 μl of polyethylene glycol 4000 BCC (50 w/v) 12.2 μl of tris chloride BCC pH 7 (1 M) 17.8 μl of tris chloride BCC pH 9 (1 M) 490.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 260.0 μl of polyethylene glycol 4000 BCC (50 w/v) 12.5 μl of sodium HEPES BCC pH 6.5 (1 M) 12.5 μl of sodium HEPES BCC pH 8.5 (1 M) 40.0 μl of sodium chloride BCC (5 M) 675.0 μl of water (100 v/v) |
B7 |
| pH 7.5 | 220.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 680.0 μl of water (100 v/v) |
B8 |
| pH 6.7 | 132.0 μl of polyethylene glycol 4000 BCC (50 w/v) 6.7 μl of potassium chloride BCC (3 M) 3.4 μl of sodium MES BCC pH 5.2 (1 M) 16.6 μl of sodium MES BCC pH 7.2 (1 M) 16.0 μl of sodium chloride BCC (5 M) 825.3 μl of water (100 v/v) |
B9 |
| pH 7 | 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 33.3 μl of potassium chloride BCC (3 M) 20.0 μl of tris chloride BCC pH 7 (1 M) 546.7 μl of water (100 v/v) |
B10 |
| pH 6.7 | 50.0 μl of magnesium chloride BCC (1 M) 132.0 μl of polyethylene glycol 3350 BCC (50 w/v) 22.7 μl of sodium cacodylate BCC pH 5.3 (1 M) 77.3 μl of sodium cacodylate BCC pH 7.3 (1 M) 718.0 μl of water (100 v/v) |
B11 |
| pH 5.5 | 740.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v) 66.7 μl of potassium chloride BCC (3 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 93.3 μl of water (100 v/v) |
B12 |
| pH 8 | 110.0 μl of polyethylene glycol 4000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 790.0 μl of water (100 v/v) |
C1 |
| pH 7 | 154.0 μl of polyethylene glycol 4000 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 20.0 μl of tris chloride BCC pH 7 (1 M) 806.0 μl of water (100 v/v) |
C2 |
| pH 7.5 | 100.0 μl of magnesium chloride BCC (1 M) 275.0 μl of polyethylene glycol 400 BCC (80 v/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 525.0 μl of water (100 v/v) |
C3 |
| pH 8 | 337.5 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 8.0 μl of sodium chloride BCC (5 M) 21.9 μl of tris chloride BCC pH 7 (1 M) 18.1 μl of tris chloride BCC pH 9 (1 M) 614.5 μl of water (100 v/v) |
C4 |
| pH 6 | 20.0 μl of magnesium chloride BCC (1 M) 275.0 μl of polyethylene glycol 400 BCC (80 v/v) 10.0 μl of sodium chloride BCC (5 M) 90.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 595.0 μl of water (100 v/v) |
C5 |
| pH 5.5 | 176.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 724.0 μl of water (100 v/v) |
C6 |
| NA | 80.0 μl of ammonium acetate BCC (5 M) 260.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 660.0 μl of water (100 v/v) |
C7 |
| pH 7 | 7.1 μl of bis-tris chloride BCC pH 6.5 (1 M) 12.9 μl of bis-tris chloride BCC pH 7.5 (1 M) 300.0 μl of polyethylene glycol 2000 BCC (50 w/v) 680.0 μl of water (100 v/v) |
C8 |
| pH 7.5 | 100.0 μl of magnesium chloride BCC (1 M) 220.0 μl of polyethylene glycol 1500 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 16.9 μl of tris chloride BCC pH 7 (1 M) 3.1 μl of tris chloride BCC pH 9 (1 M) 640.0 μl of water (100 v/v) |
C9 |
| pH 8 | 100.0 μl of magnesium chloride BCC (1 M) 220.0 μl of polyethylene glycol 1500 BCC (50 w/v) 18.3 μl of sodium HEPES BCC pH 6.5 (1 M) 81.7 μl of sodium HEPES BCC pH 8.5 (1 M) 20.0 μl of sodium chloride BCC (5 M) 560.0 μl of water (100 v/v) |
C10 |
| pH 7 | 440.0 μl of polyethylene glycol 3000 BCC (50 w/v) 66.7 μl of potassium chloride BCC (3 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of sodium acetate BCC (1 M) 193.3 μl of water (100 v/v) |
C11 |
| pH 7 | 660.0 μl of jeffamine M-600 BCC pH 7 (50 v/v) 20.0 μl of nickel(II) sulfate BCC (1 M) 8.2 μl of sodium HEPES BCC pH 6.5 (1 M) 1.8 μl of sodium HEPES BCC pH 8.5 (1 M) 310.0 μl of water (100 v/v) |
C12 |
| pH 8 | 260.0 μl of polyethylene glycol 6000 BCC (50 w/v) 30.0 μl of sodium chloride BCC (5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 610.0 μl of water (100 v/v) |
D1 |
| pH 7.5 | 200.0 μl of calcium chloride BCC (1 M) 662.5 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 37.5 μl of water (100 v/v) |
D2 |
| pH 5 | 20.0 μl of magnesium acetate BCC (2.5 M) 350.0 μl of polyethylene glycol 400 BCC (80 v/v) 32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 580.0 μl of water (100 v/v) |
D3 |
| pH 7.5 | 440.0 μl of polyethylene glycol 4000 BCC (50 w/v) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 510.0 μl of water (100 v/v) |
D4 |
| pH 8 | 200.0 μl of calcium chloride BCC (1 M) 550.0 μl of polyethylene glycol 400 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 150.0 μl of water (100 v/v) |
D5 |
| pH 5.4 | 20.0 μl of magnesium acetate BCC (2.5 M) 300.0 μl of polyethylene glycol 400 BCC (80 v/v) 43.7 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 6.3 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 630.0 μl of water (100 v/v) |
D6 |
| pH 6.5 | 200.0 μl of calcium chloride BCC (1 M) 325.0 μl of polyethylene glycol monomethyl ether 350 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 375.0 μl of water (100 v/v) |
D7 |
| pH 8.5 | 487.5 μl of polyethylene glycol 400 BCC (80 v/v) 33.3 μl of potassium chloride BCC (3 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 379.2 μl of water (100 v/v) |
D8 |
| pH 9 | 30.9 μl of glycine BCC pH 8.5 (1 M) 69.1 μl of glycine BCC pH 10 (1 M) 50.0 μl of magnesium chloride BCC (1 M) 275.0 μl of polyethylene glycol 400 BCC (80 v/v) 575.0 μl of water (100 v/v) |
D9 |
| pH 3.8 | 28.6 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of glycine BCC pH 3.8 (1 M) 280.0 μl of triethylene glycol BCC (100 v/v) 591.4 μl of water (100 v/v) |
D10 |
| pH 7.2 | 360.0 μl of polyethylene glycol 3350 BCC (50 w/v) 70.3 μl of sodium HEPES BCC pH 6.5 (1 M) 29.7 μl of sodium HEPES BCC pH 8.5 (1 M) 37.5 μl of sodium formate BCC (4 M) 502.5 μl of water (100 v/v) |
D11 |
| NA | 176.0 μl of polyethylene glycol 6000 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 624.0 μl of water (100 v/v) |
D12 |
| pH 6.5 | 360.0 μl of polyethylene glycol 6000 BCC (50 w/v) 66.7 μl of potassium chloride BCC (3 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 473.3 μl of water (100 v/v) |
E1 |
| pH 8 | 437.5 μl of polyethylene glycol 400 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 137.5 μl of trisodium citrate BCC (1.6 M) 325.0 μl of water (100 v/v) |
E2 |
| pH 4.5 | 212.5 μl of polyethylene glycol 400 BCC (80 v/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 687.5 μl of water (100 v/v) |
E3 |
| pH 8.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 36.0 μl of polyethylene glycol 8000 BCC (50 w/v) 4.2 μl of tris chloride BCC pH 7 (1 M) 15.8 μl of tris chloride BCC pH 9 (1 M) 544.0 μl of water (100 v/v) |
E4 |
| pH 7.5 | 258.8 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 16.9 μl of tris chloride BCC pH 7 (1 M) 3.1 μl of tris chloride BCC pH 9 (1 M) 721.2 μl of water (100 v/v) |
E5 |
| pH 10 | 0.4 μl of glycine BCC pH 8.5 (1 M) 19.6 μl of glycine BCC pH 10 (1 M) 660.0 μl of polyethylene glycol 1000 BCC (50 w/v) 10.0 μl of sodium chloride BCC (5 M) 310.0 μl of water (100 v/v) |
E6 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
E7 |
| pH 6.5 | 200.0 μl of magnesium chloride BCC (1 M) 620.0 μl of polyethylene glycol 2000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 80.0 μl of water (100 v/v) |
E8 |
| pH 4.6 | 360.0 μl of polyethylene glycol 3350 BCC (50 w/v) 242.7 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 397.3 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) |
E9 |
| pH 8 | 100.0 μl of cadmium chloride BCC (1 M) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 20.0 μl of sodium chloride BCC (5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 367.5 μl of water (100 v/v) |
E10 |
| pH 8.9 | 620.0 μl of polyethylene glycol 2000 BCC (50 w/v) 16.9 μl of sodium bicine BCC pH 7.4 (1 M) 83.1 μl of sodium bicine BCC pH 9.4 (1 M) 280.0 μl of water (100 v/v) |
E11 |
| pH 8.5 | 20.0 μl of lithium sulfate BCC (2.5 M) 437.5 μl of polyethylene glycol 400 BCC (80 v/v) 41.7 μl of sodium sulfate BCC (1.2 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 450.8 μl of water (100 v/v) |
E12 |
| pH 9.5 | 4.5 μl of glycine BCC pH 8.5 (1 M) 45.5 μl of glycine BCC pH 10 (1 M) 412.5 μl of polyethylene glycol 300 BCC (80 v/v) 20.0 μl of sodium chloride BCC (5 M) 517.5 μl of water (100 v/v) |
F1 |
| pH 8 | 300.0 μl of magnesium nitrate BCC (1 M) 460.0 μl of polyethylene glycol 2000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 140.0 μl of water (100 v/v) |
F2 |
| NA | 48.0 μl of lithium sulfate BCC (2.5 M) 250.0 μl of polyethylene glycol 300 BCC (80 v/v) 16.9 μl of tris chloride BCC pH 7 (1 M) 3.1 μl of tris chloride BCC pH 9 (1 M) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 582.0 μl of water (100 v/v) |
F3 |
| pH 9 | 250.0 μl of polyethylene glycol 400 BCC (80 v/v) 20.0 μl of sodium chloride BCC (5 M) 120.0 μl of tris chloride BCC pH 9 (1 M) 610.0 μl of water (100 v/v) |
F4 |
| pH 7 | 258.8 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 40.0 μl of sodium chloride BCC (5 M) 601.2 μl of water (100 v/v) |
F5 |
| pH 8 | 262.5 μl of polyethylene glycol 400 BCC (80 v/v) 325.0 μl of sodium acetate BCC (1 M) 20.0 μl of sodium chloride BCC (5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 292.5 μl of water (100 v/v) |
F6 |
| pH 6.2 | 360.0 μl of polyethylene glycol 2000 BCC (50 w/v) 64.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 16.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 12.5 μl of trisodium citrate BCC (1.6 M) 547.5 μl of water (100 v/v) |
F7 |
| NA | 154.0 μl of polyethylene glycol 4000 BCC (50 w/v) 20.0 μl of potassium nitrate BCC (1 M) 30.0 μl of tripotassium citrate BCC (1 M) 796.0 μl of water (100 v/v) |
F8 |
| pH 8.5 | 5.0 μl of magnesium chloride BCC (1 M) 600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 275.0 μl of water (100 v/v) |
F9 |
| pH 7 | 200.0 μl of calcium chloride BCC (1 M) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 287.5 μl of water (100 v/v) |
F10 |
| pH 7 | 100.0 μl of calcium chloride BCC (1 M) 260.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 540.0 μl of water (100 v/v) |
F11 |
| pH 4 | 57.1 μl of ammonium sulfate BCC (3.5 M) 412.5 μl of polyethylene glycol 200 BCC (80 v/v) 1.2 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 18.8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 4.0 μl of sodium chloride BCC (5 M) 506.4 μl of water (100 v/v) |
F12 |
| pH 4.5 | 275.0 μl of polyethylene glycol 400 BCC (80 v/v) 14.0 μl of sodium chloride BCC (5 M) 280.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 220.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 211.0 μl of water (100 v/v) |
G1 |
| pH 4.6 | 57.1 μl of ammonium sulfate BCC (3.5 M) 329.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 513.4 μl of water (100 v/v) |
G2 |
| pH 9 | 15.5 μl of glycine BCC pH 8.5 (1 M) 34.5 μl of glycine BCC pH 10 (1 M) 687.5 μl of polyethylene glycol 400 BCC (80 v/v) 262.5 μl of water (100 v/v) |
G3 |
| pH 8.5 | 100.0 μl of magnesium chloride BCC (1 M) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 20.0 μl of sodium chloride BCC (5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 367.5 μl of water (100 v/v) |
G4 |
| pH 4 | 40.0 μl of lithium sulfate BCC (2.5 M) 92.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 108.0 μl of phosphate-citrate BCC pH 9.3 (1 M) 380.0 μl of polyethylene glycol 1000 BCC (50 w/v) 380.0 μl of water (100 v/v) |
G5 |
| pH 4 | 200.0 μl of magnesium chloride BCC (1 M) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 33.3 μl of potassium chloride BCC (3 M) 10.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 14.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 329.2 μl of water (100 v/v) |
G6 |
| pH 6.1 | 220.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.3 μl of sodium MES BCC pH 5.2 (1 M) 22.7 μl of sodium MES BCC pH 7.2 (1 M) 50.0 μl of zinc acetate BCC (1 M) 680.0 μl of water (100 v/v) |
G7 |
| pH 8 | 300.0 μl of magnesium nitrate BCC (1 M) 440.0 μl of polyethylene glycol 8000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 160.0 μl of water (100 v/v) |
G8 |
| pH 6.5 | 40.0 μl of ethylene glycol BCC (100 v/v) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 20.0 μl of sodium chloride BCC (5 M) 427.5 μl of water (100 v/v) |
G9 |
| pH 5.5 | 325.0 μl of polyethylene glycol 400 BCC (80 v/v) 10.0 μl of sodium chloride BCC (5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 565.0 μl of water (100 v/v) |
G10 |
| pH 9.3 | 15.0 μl of glycine BCC pH 8.5 (1 M) 85.0 μl of glycine BCC pH 10 (1 M) 40.0 μl of lithium sulfate BCC (2.5 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 485.0 μl of water (100 v/v) |
G11 |
| NA | 440.0 μl of polyethylene glycol 6000 BCC (50 w/v) 100.0 μl of sodium dihydrogen phosphate BCC (1 M) 50.0 μl of trilithium citrate BCC (1 M) 150.0 μl of tripotassium citrate BCC (1 M) 260.0 μl of water (100 v/v) |
G12 |
| pH 7.8 | 560.0 μl of polyethylene glycol 600 BCC (50 w/v) 14.8 μl of sodium HEPES BCC pH 6.5 (1 M) 35.2 μl of sodium HEPES BCC pH 8.5 (1 M) 10.0 μl of zinc sulfate BCC (0.1 M) 380.0 μl of water (100 v/v) |
H1 |
| pH 7 | 412.5 μl of polyethylene glycol 300 BCC (80 v/v) 20.0 μl of sodium chloride BCC (5 M) 36.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M) 64.0 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M) 467.5 μl of water (100 v/v) |
H2 |
| pH 9 | 412.5 μl of polyethylene glycol 300 BCC (80 v/v) 6.2 μl of sodium bicine BCC pH 7.4 (1 M) 43.8 μl of sodium bicine BCC pH 9.4 (1 M) 20.0 μl of sodium chloride BCC (5 M) 517.5 μl of water (100 v/v) |
H3 |
| pH 6 | 60.0 μl of ethylene glycol BCC (100 v/v) 132.0 μl of polyethylene glycol 8000 BCC (50 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 50.0 μl of zinc acetate BCC (1 M) 658.0 μl of water (100 v/v) |
H4 |
| pH 3.5 | 80.0 μl of lithium sulfate BCC (2.5 M) 350.0 μl of polyethylene glycol 400 BCC (80 v/v) 30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 470.0 μl of water (100 v/v) |
H5 |
| pH 7.5 | 220.0 μl of polyethylene glycol 4000 BCC (50 w/v) 20.0 μl of sodium chloride BCC (5 M) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 660.0 μl of water (100 v/v) |
H6 |
| pH 7.4 | 20.0 μl of lithium sulfate BCC (2.5 M) 140.0 μl of polyethylene glycol 3000 BCC (50 w/v) 93.9 μl of tricine BCC pH 7.2 (1 M) 6.1 μl of tricine BCC pH 9.2 (1 M) 740.0 μl of water (100 v/v) |
H7 |
| pH 6.5 | 200.0 μl of calcium chloride BCC (1 M) 412.5 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 287.5 μl of water (100 v/v) |
H8 |
| pH 6 | 560.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of sodium chloride BCC (5 M) 90.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 10.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 140.0 μl of water (100 v/v) |
H9 |
| pH 7.5 | 220.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 680.0 μl of water (100 v/v) |
H10 |
| pH 7 | 500.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 65.4 μl of sodium HEPES BCC pH 6.5 (1 M) 14.6 μl of sodium HEPES BCC pH 8.5 (1 M) 20.0 μl of zinc sulfate BCC (0.1 M) 400.0 μl of water (100 v/v) |
H11 |
| pH 6.5 | 30.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 495.0 μl of water (100 v/v) |
H12 |
| pH 7 | 78.0 μl of bis-tris propane chloride BCC pH 6 (1 M) 22.0 μl of bis-tris propane chloride BCC pH 10 (1 M) 600.0 μl of sodium chloride BCC (5 M) 300.0 μl of water (100 v/v) |