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Supplemental Information

Screen Description

Lysozyme QA Screen

BCC Core Screens / Lysozyme QA Screen
Created 2025-06-04.
Four conditions that grow lysozyme crystals with different morphology for routine testing of robotics in BCC lab.

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Barcodes

Lysozyme QA Screen

  • Z0002749
  • Z0002750
  • Z0002795
  • Z0002796

Screen Structure

Lysozyme QA Screen

Well Count 96
Number of distinct ingredients 11
Internal diversity (score mean dist) 0.758

Screen Composition

2025-06-12T23:07:59.650570 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Lysozyme QA Screen
Buffer 120
Polymer 72
Salt 120

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Lysozyme QA Screen Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
A2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
A3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
A4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
A5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
A6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
A7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
A8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
A9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
A10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
A11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
A12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
B1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
B2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
B3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
B4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
B5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
B6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
B7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
B8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
B9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
B10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
B11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
B12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
C1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
C2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
C3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
C4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
C5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
C6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
C7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
C8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
C9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
C10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
C11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
C12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
D1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
D2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
D3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
D4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
D5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
D6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
D7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
D8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
D9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
D10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
D11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
D12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
E1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
E2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
E3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
E4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
E5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
E6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
E7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
E8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
E9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
E10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
E11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
E12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
F1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
F2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
F3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
F4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
F5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
F6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
F7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
F8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
F9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
F10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
F11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
F12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
G1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
G2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
G3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
G4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
G5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
G6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
G7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
G8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
G10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
G11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
G12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
H1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
H2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
H3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
H4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
H5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
H6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
H7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
H8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)
H9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
18 μl of sodium HEPES BCC pH 8.5 (1 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
566 μl of water (100 v/v)
H10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100 μl of lithium chloride BCC (10 M)
58 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of polyethylene glycol 6000 BCC (50 w/v)
42 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
H11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
H12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
400 μl of water (100 v/v)