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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Lysozyme QA Screen
BCC Core Screens / Lysozyme QA Screen
Created 2025-07-17.
Four conditions that grow lysozyme crystals with different morphology for routine testing of robotics in BCC lab.
Export
Lysozyme QA Screen
Barcodes
Lysozyme QA Screen
Screen Structure
Lysozyme QA Screen
Well Count | 96 |
---|---|
Number of distinct ingredients | 11 |
Internal diversity (score mean dist) | 0.758 |
Screen Composition
ingredient type | Lysozyme QA Screen |
---|---|
Buffer | 120 |
Polymer | 72 |
Salt | 120 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
A2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
A3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
A4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
A5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
A6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
A7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
A8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
A9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
A10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
A11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
A12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
B1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
B2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
B3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
B4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
B5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
B6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
B7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
B8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
B9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
B10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
B11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
B12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
C1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
C2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
C3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
C4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
C5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
C6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
C7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
C8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
C9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
C10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
C11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
C12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
D1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
D2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
D3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
D4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
D5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
D6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
D7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
D8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
D9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
D10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
D11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
D12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
E1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
E2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
E3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
E4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
E5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
E6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
E7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
E8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
E9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
E10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
E11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
E12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
F1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
F2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
F3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
F4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
F5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
F6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
F7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
F8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
F9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
F10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
F11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
F12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
G1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
G2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
G3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
G4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
G5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
G6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
G7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
G8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
G9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
G10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
G11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
G12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
H1 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
H2 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
H3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
H4 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
H5 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
H6 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
H7 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
H8 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |
H9 |
| pH 7 | 10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
H10 |
| pH 4 | 100.0 μl of lithium chloride BCC (10 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
H11 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
H12 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) 400.0 μl of water (100 v/v) |