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Supplemental Information

Screen Description

Lysozyme QA Screen

BCC Core Screens / Lysozyme QA Screen
Created 2025-07-17.
Four conditions that grow lysozyme crystals with different morphology for routine testing of robotics in BCC lab.

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Lysozyme QA Screen

  • Z0002830 Lot 5 (1500.0 µl)
  • Z0002831 Lot 6 (1500.0 µl)
  • Z0002832 Lot 7 (1500.0 µl)
  • Z0002749 Lot 1 (0.0 µl)
  • Z0002750 Lot 2 (0.0 µl)
  • Z0002795 Lot 3 (0.0 µl)
  • Z0002796 Lot 4 (0.0 µl)

Screen Structure

Lysozyme QA Screen

Well Count 96
Number of distinct ingredients 11
Internal diversity (score mean dist) 0.758

Screen Composition

2025-08-15T11:09:52.020709 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Lysozyme QA Screen
Buffer 120
Polymer 72
Salt 120

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Lysozyme QA Screen Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
A2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
A3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
A4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
A5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
A6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
A7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
A8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
A9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
A10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
A11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
A12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
B1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
B2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
B3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
B4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
B5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
B6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
B7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
B8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
B9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
B10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
B11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
B12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
C1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
C2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
C3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
C4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
C5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
C6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
C7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
C8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
C9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
C10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
C11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
C12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
D1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
D2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
D3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
D5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
D6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
D7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
D9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
D10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
D11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
E1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
E2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
E3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
E4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
E5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
E6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
E7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
E8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
E9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
E10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
E11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
E12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
F1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
F2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
F3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
F5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
F6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
F7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
F9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
F10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
F11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
G1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
G2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
G3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
G4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
G5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
G6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
G7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
G8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
G10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
G11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
G12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
H1
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
H2
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
H3
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
H4
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
H5
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
H6
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
H7
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
H8
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)
H9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M -
pH 7
10.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
323.5 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
566.5 μl of water (100 v/v)
H10
  • lithium chloride BCC 1 M -
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4
pH 4
100.0 μl of lithium chloride BCC (10 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
42.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
58.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
H11
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
H12
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
400.0 μl of water (100 v/v)