Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Created 2025-08-28.
C3's take on the LCP screen designed by Martin Caffrey and published in 2015, now with buffered tricine in well E12

Rigaku Recipe XML

μl
Stocks

Export

Barcodes

  • Z0002839 Lot 5 (1500.0 µl)
  • Z0002840 Lot 6 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 74
Internal diversity (score mean dist) 0.887

Screen Composition

2025-10-20T20:58:08.560335 image/svg+xml Matplotlib v3.10.6, https://matplotlib.org/
ingredient type LCP_C3
Buffer 106
Cryoprotectant 4
Organic (non-volatile) 13
Organic (volatile) 11
Polymer 93
Salt 128

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
LCP_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • polyethylene glycol 300 BCC 15 v/v -
  • sodium HEPES BCC 0.1 M pH 7.6
  • disodium EDTA BCC 0.05 M -
  • 1,7-heptanediol BCC 1.2 v/v -
pH 7.6
12.6 μl of 1,7-heptanediol BCC (95 v/v)
100.0 μl of disodium EDTA BCC (0.5 M)
187.5 μl of polyethylene glycol 300 BCC (80 v/v)
43.0 μl of sodium HEPES BCC pH 6.5 (1 M)
57.0 μl of sodium HEPES BCC pH 8.5 (1 M)
599.9 μl of water (100 v/v)
A2
  • 2-methyl-2,4-pentanediol BCC 2 v/v -
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • polyethylene glycol 300 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
375.0 μl of polyethylene glycol 300 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
465.0 μl of water (100 v/v)
A3
  • polyethylene glycol 300 BCC 30 v/v -
  • sodium chloride BCC 0.15 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 6.3
pH 6.3
375.0 μl of polyethylene glycol 300 BCC (80 v/v)
150.0 μl of sodium chloride BCC (1 M)
75.7 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
24.3 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
375.0 μl of water (100 v/v)
A4
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • polyethylene glycol 300 BCC 32 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • 1,7-heptanediol BCC 1 v/v -
pH 7.5
10.5 μl of 1,7-heptanediol BCC (95 v/v)
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400.0 μl of polyethylene glycol 300 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
449.5 μl of water (100 v/v)
A5
  • diammonium hydrogen citrate BCC 0.1 M -
  • polyethylene glycol 300 BCC 37 v/v -
  • bis-tris propane chloride BCC 0.1 M pH 6.5
pH 6.5
94.3 μl of bis-tris propane chloride BCC pH 6 (1 M)
5.7 μl of bis-tris propane chloride BCC pH 10 (1 M)
50.0 μl of diammonium hydrogen citrate BCC (2 M)
462.5 μl of polyethylene glycol 300 BCC (80 v/v)
387.5 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 0.15 M -
  • polyethylene glycol 400 BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
150.0 μl of ammonium sulfate BCC (1 M)
125.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
A7
  • jeffamine M-600 BCC 0.45 v/v -
  • polyethylene glycol 400 BCC 13 v/v -
  • potassium sodium tartrate BCC 0.17 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
9.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
162.5 μl of polyethylene glycol 400 BCC (80 v/v)
85.0 μl of potassium sodium tartrate BCC (2 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
643.5 μl of water (100 v/v)
A8
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
  • polyethylene glycol 400 BCC 14 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
175.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
665.0 μl of water (100 v/v)
A9
  • polyethylene glycol 400 BCC 18 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • trisodium citrate BCC 0.1 M -
pH 7.5
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of trisodium citrate BCC (1 M)
575.0 μl of water (100 v/v)
A10
  • polyethylene glycol 400 BCC 18 v/v -
  • potassium sulfate BCC 0.1 M -
  • sodium HEPES BCC 0.05 M pH 7
pH 7
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
200.0 μl of potassium sulfate BCC (0.5 M)
40.9 μl of sodium HEPES BCC pH 6.5 (1 M)
9.1 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
A11
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 400 BCC 18.5 v/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
50.0 μl of lithium sulfate BCC (1 M)
231.2 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
518.8 μl of water (100 v/v)
A12
  • ammonium dihydrogen phosphate BCC 0.35 M -
  • polyethylene glycol 400 BCC 19.5 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
140.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
243.8 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
516.2 μl of water (100 v/v)
B1
  • polyethylene glycol 400 BCC 20 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate BCC 0.3 M -
  • taurine BCC 0.005 M -
pH 5.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
88.2 μl of sodium malonate BCC (3.4 M)
50.0 μl of taurine BCC (0.1 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
511.8 μl of water (100 v/v)
B2
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium chloride BCC 0.4 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of sodium chloride BCC (1 M)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
B3
  • polyethylene glycol 400 BCC 20 v/v -
  • potassium nitrate BCC 0.4 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of potassium nitrate BCC (1 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
250.0 μl of water (100 v/v)
B4
  • diammonium hydrogen citrate BCC 0.07 M -
  • polyethylene glycol 400 BCC 21 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
35.0 μl of diammonium hydrogen citrate BCC (2 M)
262.5 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
602.5 μl of water (100 v/v)
B5
  • nickel(II) chloride BCC 0.005 M -
  • polyethylene glycol 400 BCC 23 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.25
  • sodium malonate BCC 0.3 M -
pH 5.25
5.0 μl of nickel(II) chloride BCC (1 M)
287.5 μl of polyethylene glycol 400 BCC (80 v/v)
88.2 μl of sodium malonate BCC (3.4 M)
73.1 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
26.9 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
519.3 μl of water (100 v/v)
B6
  • polyethylene glycol 400 BCC 25 v/v -
  • potassium sodium tartrate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
312.5 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of potassium sodium tartrate BCC (2 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
487.5 μl of water (100 v/v)
B7
  • 1,4-butanediol BCC 3.5 v/v -
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium MES BCC 0.1 M pH 6.7
  • dimethyl sulfoxide BCC 4 v/v -
pH 6.7
35.0 μl of 1,4-butanediol BCC (100 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
200.0 μl of lithium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
16.9 μl of sodium MES BCC pH 5.2 (1 M)
83.1 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
B8
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium MES BCC 0.1 M pH 6
  • strontium chloride BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
pH 6
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
88.2 μl of sodium malonate BCC (3.4 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
5.0 μl of strontium chloride BCC (1 M)
481.8 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 0.3 M -
  • polyethylene glycol 400 BCC 26 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of ammonium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
275.0 μl of water (100 v/v)
B10
  • polyethylene glycol 400 BCC 26.5 v/v -
  • sodium thiocyanate BCC 0.05 M -
  • 2,5-hexanediol BCC 2 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
40.0 μl of 2,5-hexanediol BCC (50 v/v)
331.2 μl of polyethylene glycol 400 BCC (80 v/v)
25.0 μl of sodium thiocyanate BCC (2 M)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
503.8 μl of water (100 v/v)
B11
  • 1,4-butanediol BCC 5 v/v -
  • polyethylene glycol 400 BCC 27 v/v -
  • sodium formate BCC 0.22 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
55.0 μl of sodium formate BCC (4 M)
71.1 μl of tris chloride BCC pH 7 (1 M)
28.9 μl of tris chloride BCC pH 9 (1 M)
457.5 μl of water (100 v/v)
B12
  • ammonium fluoride BCC 0.15 M -
  • jeffamine M-600 BCC 2.5 v/v -
  • polyethylene glycol 400 BCC 27 v/v -
  • sodium HEPES BCC 0.1 M pH 6.9
pH 6.9
150.0 μl of ammonium fluoride BCC (1 M)
50.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
86.6 μl of sodium HEPES BCC pH 6.5 (1 M)
13.4 μl of sodium HEPES BCC pH 8.5 (1 M)
362.5 μl of water (100 v/v)
C1
  • polyethylene glycol 400 BCC 27.5 v/v -
  • 1,3-butanediol BCC 3 v/v -
  • disodium tartrate BCC 0.12 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.7
pH 6.7
30.0 μl of 1,3-butanediol BCC (100 v/v)
87.0 μl of bis-tris propane chloride BCC pH 6 (1 M)
13.0 μl of bis-tris propane chloride BCC pH 10 (1 M)
120.0 μl of disodium tartrate BCC (1 M)
343.8 μl of polyethylene glycol 400 BCC (80 v/v)
406.2 μl of water (100 v/v)
C2
  • polyethylene glycol 400 BCC 27.5 v/v -
  • potassium sodium tartrate BCC 0.15 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.4
pH 6.4
95.6 μl of bis-tris propane chloride BCC pH 6 (1 M)
4.4 μl of bis-tris propane chloride BCC pH 10 (1 M)
343.8 μl of polyethylene glycol 400 BCC (80 v/v)
75.0 μl of potassium sodium tartrate BCC (2 M)
481.2 μl of water (100 v/v)
C3
  • polyethylene glycol 400 BCC 28 v/v -
  • potassium formate BCC 0.3 M -
  • bis-tris propane chloride BCC 0.1 M pH 7
pH 7
77.2 μl of bis-tris propane chloride BCC pH 6 (1 M)
22.8 μl of bis-tris propane chloride BCC pH 10 (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
300.0 μl of potassium formate BCC (1 M)
250.0 μl of water (100 v/v)
C4
  • ammonium dihydrogen phosphate BCC 0.3 M -
  • magnesium chloride BCC 0.01 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
120.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
10.0 μl of magnesium chloride BCC (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
55.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
420.0 μl of water (100 v/v)
C5
  • diammonium hydrogen citrate BCC 0.1 M -
  • polyethylene glycol 400 BCC 28.5 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
50.0 μl of diammonium hydrogen citrate BCC (2 M)
356.2 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
493.8 μl of water (100 v/v)
C6
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 400 BCC 29 v/v -
  • sodium MES BCC 0.1 M pH 6.8
pH 6.8
100.0 μl of diammonium hydrogen citrate BCC (2 M)
362.5 μl of polyethylene glycol 400 BCC (80 v/v)
12.4 μl of sodium MES BCC pH 5.2 (1 M)
87.6 μl of sodium MES BCC pH 7.2 (1 M)
437.5 μl of water (100 v/v)
C7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 29 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 4
pH 4
200.0 μl of lithium sulfate BCC (1 M)
362.5 μl of polyethylene glycol 400 BCC (80 v/v)
21.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
29.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
387.5 μl of water (100 v/v)
C8
  • 1,4-butanediol BCC 5 v/v -
  • polyethylene glycol 400 BCC 29.5 v/v -
  • sodium formate BCC 0.35 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
368.8 μl of polyethylene glycol 400 BCC (80 v/v)
87.5 μl of sodium formate BCC (4 M)
71.1 μl of tris chloride BCC pH 7 (1 M)
28.9 μl of tris chloride BCC pH 9 (1 M)
393.8 μl of water (100 v/v)
C9
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • sodium malonate BCC 0.2 M -
pH 8
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
58.8 μl of sodium malonate BCC (3.4 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
466.2 μl of water (100 v/v)
C10
  • lithium sulfate BCC 0.185 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 6.5
pH 6.5
185.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
96.8 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
3.2 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
340.0 μl of water (100 v/v)
C11
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
C12
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
D1
  • ammonium acetate BCC 0.3 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • glucose BCC 0.11 M -
  • bis-tris propane chloride BCC 0.1 M pH 7.5
pH 7.5
60.0 μl of ammonium acetate BCC (5 M)
61.7 μl of bis-tris propane chloride BCC pH 6 (1 M)
38.3 μl of bis-tris propane chloride BCC pH 10 (1 M)
110.0 μl of glucose BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
355.0 μl of water (100 v/v)
D2
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
425.0 μl of water (100 v/v)
D3
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6
  • polypropylene glycol P400 BCC 2.5 v/v -
  • magnesium sulfate BCC 0.1 M -
pH 6
100.0 μl of magnesium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
41.7 μl of polypropylene glycol P400 BCC (60 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
383.3 μl of water (100 v/v)
D4
  • polyethylene glycol 400 BCC 30 v/v -
  • potassium nitrate BCC 0.4 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of potassium nitrate BCC (1 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
125.0 μl of water (100 v/v)
D5
  • lithium chloride BCC 0.4 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of lithium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
125.0 μl of water (100 v/v)
D6
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • magnesium sulfate BCC 0.1 M -
pH 8
100.0 μl of magnesium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
425.0 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.3
  • ammonium formate BCC 0.1 M -
pH 6.3
100.0 μl of ammonium formate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.7 μl of sodium MES BCC pH 5.2 (1 M)
59.3 μl of sodium MES BCC pH 7.2 (1 M)
425.0 μl of water (100 v/v)
D8
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7
  • sodium thiocyanate BCC 0.1 M -
pH 7
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
475.0 μl of water (100 v/v)
D9
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • sodium thiocyanate BCC 0.1 M -
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
455.0 μl of water (100 v/v)
D10
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium thiocyanate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium thiocyanate BCC (2 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
455.0 μl of water (100 v/v)
D11
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • trehalose BCC 0.09 M -
pH 5.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
90.0 μl of trehalose BCC (1 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
335.0 μl of water (100 v/v)
D12
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
100.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
475.0 μl of water (100 v/v)
E1
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
E2
  • polyethylene glycol 400 BCC 31 v/v -
  • sodium MES BCC 0.1 M pH 6
  • hexammine cobalt(III) chloride BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
pH 6
50.0 μl of hexammine cobalt(III) chloride BCC (0.1 M)
387.5 μl of polyethylene glycol 400 BCC (80 v/v)
88.2 μl of sodium malonate BCC (3.4 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
374.3 μl of water (100 v/v)
E3
  • magnesium chloride BCC 0.35 M -
  • polyethylene glycol 400 BCC 31 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 0.15 M -
pH 7.5
350.0 μl of magnesium chloride BCC (1 M)
387.5 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
150.0 μl of trisodium citrate BCC (1 M)
12.5 μl of water (100 v/v)
E4
  • ammonium fluoride BCC 0.07 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium HEPES BCC 0.1 M pH 7.8
  • polypropylene glycol P400 BCC 6 v/v -
pH 7.8
70.0 μl of ammonium fluoride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of polypropylene glycol P400 BCC (60 v/v)
29.7 μl of sodium HEPES BCC pH 6.5 (1 M)
70.3 μl of sodium HEPES BCC pH 8.5 (1 M)
330.0 μl of water (100 v/v)
E5
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E6
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium MES BCC 0.05 M pH 6.5
  • sodium thiocyanate BCC 0.1 M -
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
480.0 μl of water (100 v/v)
E7
  • magnesium chloride BCC 0.13 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium chloride BCC 0.075 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
130.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
75.0 μl of sodium chloride BCC (1 M)
55.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
295.0 μl of water (100 v/v)
E8
  • 1,4-butanediol BCC 6 v/v -
  • polyethylene glycol 400 BCC 32.5 v/v -
  • sodium sulfate BCC 0.15 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.75
pH 6.75
60.0 μl of 1,4-butanediol BCC (100 v/v)
85.6 μl of bis-tris propane chloride BCC pH 6 (1 M)
14.4 μl of bis-tris propane chloride BCC pH 10 (1 M)
406.2 μl of polyethylene glycol 400 BCC (80 v/v)
125.0 μl of sodium sulfate BCC (1.2 M)
308.8 μl of water (100 v/v)
E9
  • ethylene glycol BCC 5 v/v -
  • polyethylene glycol 400 BCC 32.5 v/v -
  • potassium sodium tartrate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.2
pH 6.2
50.0 μl of ethylene glycol BCC (100 v/v)
406.2 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of potassium sodium tartrate BCC (2 M)
47.6 μl of sodium MES BCC pH 5.2 (1 M)
52.4 μl of sodium MES BCC pH 7.2 (1 M)
393.8 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 0.12 M -
  • polyethylene glycol 400 BCC 34 v/v -
  • tris chloride BCC 0.1 M pH 8.7
  • trisodium citrate BCC 0.09 M -
pH 8.7
120.0 μl of ammonium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
10.9 μl of tris chloride BCC pH 7 (1 M)
89.1 μl of tris chloride BCC pH 9 (1 M)
90.0 μl of trisodium citrate BCC (1 M)
265.0 μl of water (100 v/v)
E11
  • lithium sulfate BCC 0.3 M -
  • polyethylene glycol 400 BCC 34 v/v -
  • sodium HEPES BCC 0.1 M pH 7
  • dimethyl sulfoxide BCC 7.5 v/v -
pH 7
75.0 μl of dimethyl sulfoxide BCC (100 v/v)
300.0 μl of lithium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
E12
  • glycerol BCC 4 v/v -
  • polyethylene glycol 400 BCC 35 v/v -
  • tricine BCC 0.1 M pH 8.2
  • trisodium citrate BCC 0.08 M -
pH 8.2
50.0 μl of glycerol BCC (80 v/v)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
47.6 μl of tricine BCC pH 7.2 (1 M)
52.4 μl of tricine BCC pH 9.2 (1 M)
80.0 μl of trisodium citrate BCC (1 M)
332.5 μl of water (100 v/v)
F1
  • ammonium acetate BCC 0.37 M -
  • polyethylene glycol 400 BCC 35 v/v -
  • 1-propanol BCC 3 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
37.5 μl of 1-propanol BCC (80 v/v)
74.0 μl of ammonium acetate BCC (5 M)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
351.0 μl of water (100 v/v)
F2
  • ammonium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 36 v/v -
  • sodium HEPES BCC 0.1 M pH 7.2
pH 7.2
100.0 μl of ammonium chloride BCC (1 M)
450.0 μl of polyethylene glycol 400 BCC (80 v/v)
70.3 μl of sodium HEPES BCC pH 6.5 (1 M)
29.7 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
F3
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • polyethylene glycol 400 BCC 37.5 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
  • diammonium tartrate BCC 0.18 M -
pH 6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
90.0 μl of diammonium tartrate BCC (2 M)
468.8 μl of polyethylene glycol 400 BCC (80 v/v)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
301.2 μl of water (100 v/v)
F4
  • lithium chloride BCC 0.3 M -
  • polyethylene glycol 400 BCC 38 v/v -
  • sodium HEPES BCC 0.1 M pH 7
  • strontium chloride BCC 0.03 M -
pH 7
300.0 μl of lithium chloride BCC (1 M)
475.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
30.0 μl of strontium chloride BCC (1 M)
95.0 μl of water (100 v/v)
F5
  • polyethylene glycol 400 BCC 39 v/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
487.5 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of potassium chloride BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
312.5 μl of water (100 v/v)
F6
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • polyethylene glycol 400 BCC 39.8 v/v -
  • bis-tris propane chloride BCC 0.1 M pH 7.2
pH 7.2
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
70.3 μl of bis-tris propane chloride BCC pH 6 (1 M)
29.7 μl of bis-tris propane chloride BCC pH 10 (1 M)
497.5 μl of polyethylene glycol 400 BCC (80 v/v)
362.5 μl of water (100 v/v)
F7
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
  • dimethyl sulfoxide BCC 4 v/v -
  • 1,7-heptanediol BCC 1 v/v -
pH 8
10.5 μl of 1,7-heptanediol BCC (95 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
349.5 μl of water (100 v/v)
F8
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium chloride BCC 1.6 M -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
320.0 μl of sodium chloride BCC (5 M)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
130.0 μl of water (100 v/v)
F9
  • ADA BCC 0.1 M pH 6.5
  • lithium chloride BCC 0.16 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • strontium chloride BCC 0.004 M -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
160.0 μl of lithium chloride BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
4.0 μl of strontium chloride BCC (1 M)
136.0 μl of water (100 v/v)
F10
  • ADA BCC 0.1 M pH 6.5
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of water (100 v/v)
F11
  • polyethylene glycol 400 BCC 42 v/v -
  • sodium acetate BCC 0.15 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
525.0 μl of polyethylene glycol 400 BCC (80 v/v)
150.0 μl of sodium acetate BCC (1 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
225.0 μl of water (100 v/v)
F12
  • sodium MES BCC 0.1 M pH 6
  • copper(II) chloride BCC 0.01 M -
  • ammonium formate BCC 0.2 M -
  • polyethylene glycol dimethyl ether 500 BCC 25 v/v -
pH 6
200.0 μl of ammonium formate BCC (1 M)
10.0 μl of copper(II) chloride BCC (1 M)
312.5 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
377.5 μl of water (100 v/v)
G1
  • cadmium chloride BCC 0.002 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol dimethyl ether 500 BCC 30 v/v -
pH 6
20.0 μl of cadmium chloride BCC (0.1 M)
375.0 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
505.0 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 0.1 M -
  • magnesium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
  • polyethylene glycol dimethyl ether 500 BCC 30 v/v -
pH 6
100.0 μl of ammonium sulfate BCC (1 M)
100.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
225.0 μl of water (100 v/v)
G3
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 550 BCC 14 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of lithium sulfate BCC (1 M)
164.7 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
535.3 μl of water (100 v/v)
G4
  • polyethylene glycol monomethyl ether 550 BCC 15 v/v -
  • potassium sodium tartrate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
176.5 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of potassium sodium tartrate BCC (2 M)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
623.5 μl of water (100 v/v)
G5
  • ADA BCC 0.05 M pH 6.25
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • sodium nitrate BCC 0.35 M -
  • sodium malonate-malonic acid BCC 0.05 M -
pH 6.25
71.1 μl of ADA BCC pH 5.5 (0.5 M)
28.9 μl of ADA BCC pH 7.5 (0.5 M)
294.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
14.7 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
350.0 μl of sodium nitrate BCC (1 M)
241.2 μl of water (100 v/v)
G6
  • lithium sulfate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of lithium sulfate BCC (1 M)
329.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
470.6 μl of water (100 v/v)
G7
  • ADA BCC 0.05 M pH 7
  • ammonium sulfate BCC 0.55 M -
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
pH 7
21.2 μl of ADA BCC pH 5.5 (0.5 M)
78.8 μl of ADA BCC pH 7.5 (0.5 M)
550.0 μl of ammonium sulfate BCC (1 M)
329.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.6 μl of water (100 v/v)
G8
  • ADA BCC 0.1 M pH 7
  • polyethylene glycol 600 BCC 25 w/v -
pH 7
42.5 μl of ADA BCC pH 5.5 (0.5 M)
157.5 μl of ADA BCC pH 7.5 (0.5 M)
500.0 μl of polyethylene glycol 600 BCC (50 w/v)
300.0 μl of water (100 v/v)
G9
  • polyethylene glycol 1500 BCC 22.5 w/v -
  • sodium acetate BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
450.0 μl of polyethylene glycol 1500 BCC (50 w/v)
300.0 μl of sodium acetate BCC (1 M)
90.0 μl of sodium MES BCC pH 5.2 (1 M)
10.0 μl of sodium MES BCC pH 7.2 (1 M)
150.0 μl of water (100 v/v)
G10
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
240.0 μl of polyethylene glycol 3350 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
58.8 μl of sodium malonate BCC (3.4 M)
601.2 μl of water (100 v/v)
G11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of water (100 v/v)
G12
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H1
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
  • potassium chloride BCC 0.2 M -
  • polyethylene glycol 4000 BCC 10 w/v -
pH 4.6
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
200.0 μl of potassium chloride BCC (1 M)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of water (100 v/v)
H2
  • polyethylene glycol 6000 BCC 20 w/v -
  • sodium bicine BCC 0.1 M pH 9
pH 9
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
12.4 μl of sodium bicine BCC pH 7.4 (1 M)
87.6 μl of sodium bicine BCC pH 9.4 (1 M)
500.0 μl of water (100 v/v)
H3
  • polyethylene glycol 8000 BCC 30 w/v -
  • ammonium sulfate BCC 0.2 M -
NA
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of water (100 v/v)
H4
  • sodium MES BCC 0.1 M pH 6.5
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 10 w/v -
  • ammonium chloride BCC 0.1 M -
  • calcium chloride BCC 0.01 M -
pH 6.5
100.0 μl of ammonium chloride BCC (1 M)
10.0 μl of calcium chloride BCC (1 M)
200.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
590.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 20 w/v -
  • potassium formate BCC 0.1 M -
pH 8
400.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
100.0 μl of potassium formate BCC (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
H6
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • lithium nitrate BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
pH 5.6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of lithium nitrate BCC (1 M)
20.0 μl of sodium chloride BCC (5 M)
81.4 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.6 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
740.0 μl of water (100 v/v)
H7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • magnesium acetate BCC 0.06 M -
  • sodium chloride BCC 0.1 M -
pH 5.6
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
24.0 μl of magnesium acetate BCC (2.5 M)
20.0 μl of sodium chloride BCC (5 M)
81.4 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.6 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
806.0 μl of water (100 v/v)
H8
  • ADA BCC 0.1 M pH 6.7
  • 2-methyl-2,4-pentanediol BCC 8 v/v -
  • tripotassium citrate BCC 0.1 M -
  • potassium nitrate BCC 0.4 M -
pH 6.7
80.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
81.2 μl of ADA BCC pH 5.5 (0.5 M)
118.8 μl of ADA BCC pH 7.5 (0.5 M)
400.0 μl of potassium nitrate BCC (1 M)
100.0 μl of tripotassium citrate BCC (1 M)
220.0 μl of water (100 v/v)
H9
  • trehalose BCC 0.3 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1 M pH 5.1
pH 5.1
328.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
4.6 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
300.0 μl of trehalose BCC (1 M)
366.7 μl of water (100 v/v)
H10
  • sodium acetate-acetic acid BCC 0.075 M pH 4.6
  • sodium chloride BCC 1.5 M -
pH 4.6
28.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
46.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
300.0 μl of sodium chloride BCC (5 M)
625.0 μl of water (100 v/v)
H11
  • sodium MES BCC 0.1 M pH 6.5
  • sodium acetate BCC 1 M -
pH 6.5
333.3 μl of sodium acetate BCC (3 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
566.7 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • lithium sulfate BCC 1 M -
  • nickel(II) chloride BCC 0.01 M -
pH 8.5
400.0 μl of lithium sulfate BCC (2.5 M)
10.0 μl of nickel(II) chloride BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)