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Supplemental Information

Screen Description

Created 2025-12-03.
C3's take on the LCP screen designed by Martin Caffrey and published in 2015, now with buffered tricine in well E12

Rigaku Recipe XML

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Barcodes

  • Z0002839 Lot 5 (1500.0 µl)
  • Z0002840 Lot 6 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 74
Internal diversity (Score mean dist)0.88
Internal diversity (Ion mean dist)0.84
Internal diversity (PEG mean dist)0.8
Internal diversity (Known pH mean dist)0.72
Internal diversity (Penalty mean dist)0.84

Screen Composition

Chemical classes bar chart
ingredient type LCP_C3
Buffer 106
Cryoprotectant 4
Organic (non-volatile) 13
Organic (volatile) 11
Polymer 93
Salt 128

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
LCP_C3 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • 1,7-heptanediol BCC 1.2 v/v -
  • polyethylene glycol 300 BCC 15 v/v -
  • disodium EDTA BCC 0.05 M -
  • sodium HEPES BCC 0.1 M pH 7.6
pH 7.6
12.63 μl of 1,7-heptanediol BCC (95 v/v)
100.0 μl of disodium EDTA BCC (0.5 M)
187.5 μl of polyethylene glycol 300 BCC (80 v/v)
43.0 μl of sodium HEPES BCC pH 6.5 (1 M)
57.0 μl of sodium HEPES BCC pH 8.5 (1 M)
599.87 μl of water (100 v/v)
A2
  • polyethylene glycol 300 BCC 30 v/v -
  • 2-methyl-2,4-pentanediol BCC 2 v/v -
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
375.0 μl of polyethylene glycol 300 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
465.0 μl of water (100 v/v)
A3
  • polyethylene glycol 300 BCC 30 v/v -
  • sodium chloride BCC 0.15 M -
  • sodium dihydrogen phosphate-disodium hydrogen phosphate BCC 0.1 M pH 6.3
pH 6.3
375.0 μl of polyethylene glycol 300 BCC (80 v/v)
150.0 μl of sodium chloride BCC (1 M)
75.67 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 4.6 (1 M)
24.33 μl of sodium dihydrogen phosphate-disodium hydrogen phosphate BCC pH 9.3 (1 M)
375.0 μl of water (100 v/v)
A4
  • polyethylene glycol 300 BCC 32 v/v -
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • 1,7-heptanediol BCC 1 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
10.53 μl of 1,7-heptanediol BCC (95 v/v)
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400.0 μl of polyethylene glycol 300 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
449.47 μl of water (100 v/v)
A5
  • polyethylene glycol 300 BCC 37 v/v -
  • diammonium hydrogen citrate BCC 0.1 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.5
pH 6.5
94.27 μl of bis-tris propane chloride BCC pH 6 (1 M)
5.73 μl of bis-tris propane chloride BCC pH 10 (1 M)
50.0 μl of diammonium hydrogen citrate BCC (2 M)
462.5 μl of polyethylene glycol 300 BCC (80 v/v)
387.5 μl of water (100 v/v)
A6
  • polyethylene glycol 400 BCC 10 v/v -
  • ammonium sulfate BCC 0.15 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
150.0 μl of ammonium sulfate BCC (1 M)
125.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
A7
  • polyethylene glycol 400 BCC 13 v/v -
  • jeffamine M-600 BCC 0.45 v/v -
  • potassium sodium tartrate BCC 0.17 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
9.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
162.5 μl of polyethylene glycol 400 BCC (80 v/v)
85.0 μl of potassium sodium tartrate BCC (2 M)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
643.5 μl of water (100 v/v)
A8
  • polyethylene glycol 400 BCC 14 v/v -
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
175.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.39 μl of tris chloride BCC pH 7 (1 M)
15.61 μl of tris chloride BCC pH 9 (1 M)
665.0 μl of water (100 v/v)
A9
  • polyethylene glycol 400 BCC 18 v/v -
  • trisodium citrate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.39 μl of tris chloride BCC pH 7 (1 M)
15.61 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of trisodium citrate BCC (1 M)
575.0 μl of water (100 v/v)
A10
  • polyethylene glycol 400 BCC 18 v/v -
  • potassium sulfate BCC 0.1 M -
  • sodium HEPES BCC 0.05 M pH 7
pH 7
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
200.0 μl of potassium sulfate BCC (0.5 M)
40.87 μl of sodium HEPES BCC pH 6.5 (1 M)
9.13 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
A11
  • polyethylene glycol 400 BCC 18.5 v/v -
  • lithium sulfate BCC 0.05 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
50.0 μl of lithium sulfate BCC (1 M)
231.25 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
67.33 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.67 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
518.75 μl of water (100 v/v)
A12
  • polyethylene glycol 400 BCC 19.5 v/v -
  • ammonium dihydrogen phosphate BCC 0.35 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
140.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
243.75 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
516.25 μl of water (100 v/v)
B1
  • polyethylene glycol 400 BCC 20 v/v -
  • taurine BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
88.24 μl of sodium malonate BCC (3.4 M)
50.0 μl of taurine BCC (0.1 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
511.76 μl of water (100 v/v)
B2
  • polyethylene glycol 400 BCC 20 v/v -
  • sodium chloride BCC 0.4 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of sodium chloride BCC (1 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
B3
  • polyethylene glycol 400 BCC 20 v/v -
  • potassium nitrate BCC 0.4 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
250.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of potassium nitrate BCC (1 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
250.0 μl of water (100 v/v)
B4
  • polyethylene glycol 400 BCC 21 v/v -
  • diammonium hydrogen citrate BCC 0.07 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
35.0 μl of diammonium hydrogen citrate BCC (2 M)
262.5 μl of polyethylene glycol 400 BCC (80 v/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
602.5 μl of water (100 v/v)
B5
  • polyethylene glycol 400 BCC 23 v/v -
  • nickel(II) chloride BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.25
pH 5.25
5.0 μl of nickel(II) chloride BCC (1 M)
287.5 μl of polyethylene glycol 400 BCC (80 v/v)
88.24 μl of sodium malonate BCC (3.4 M)
73.14 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
26.86 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
519.26 μl of water (100 v/v)
B6
  • polyethylene glycol 400 BCC 25 v/v -
  • potassium sodium tartrate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
312.5 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of potassium sodium tartrate BCC (2 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
487.5 μl of water (100 v/v)
B7
  • polyethylene glycol 400 BCC 26 v/v -
  • 1,4-butanediol BCC 3.5 v/v -
  • lithium sulfate BCC 0.2 M -
  • dimethyl sulfoxide BCC 4 v/v -
  • sodium MES BCC 0.1 M pH 6.7
pH 6.7
35.0 μl of 1,4-butanediol BCC (100 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
200.0 μl of lithium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
16.89 μl of sodium MES BCC pH 5.2 (1 M)
83.11 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
B8
  • polyethylene glycol 400 BCC 26 v/v -
  • strontium chloride BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
88.24 μl of sodium malonate BCC (3.4 M)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
5.0 μl of strontium chloride BCC (1 M)
481.76 μl of water (100 v/v)
B9
  • polyethylene glycol 400 BCC 26 v/v -
  • ammonium sulfate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of ammonium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
275.0 μl of water (100 v/v)
B10
  • polyethylene glycol 400 BCC 26.5 v/v -
  • sodium thiocyanate BCC 0.05 M -
  • 2,5-hexanediol BCC 2 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
40.0 μl of 2,5-hexanediol BCC (50 v/v)
331.25 μl of polyethylene glycol 400 BCC (80 v/v)
25.0 μl of sodium thiocyanate BCC (2 M)
67.33 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.67 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
503.75 μl of water (100 v/v)
B11
  • polyethylene glycol 400 BCC 27 v/v -
  • 1,4-butanediol BCC 5 v/v -
  • sodium formate BCC 0.22 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
55.0 μl of sodium formate BCC (4 M)
71.13 μl of tris chloride BCC pH 7 (1 M)
28.87 μl of tris chloride BCC pH 9 (1 M)
457.5 μl of water (100 v/v)
B12
  • polyethylene glycol 400 BCC 27 v/v -
  • ammonium fluoride BCC 0.15 M -
  • jeffamine M-600 BCC 2.5 v/v -
  • sodium HEPES BCC 0.1 M pH 6.9
pH 6.9
150.0 μl of ammonium fluoride BCC (1 M)
50.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
86.57 μl of sodium HEPES BCC pH 6.5 (1 M)
13.43 μl of sodium HEPES BCC pH 8.5 (1 M)
362.5 μl of water (100 v/v)
C1
  • polyethylene glycol 400 BCC 27.5 v/v -
  • 1,3-butanediol BCC 3 v/v -
  • disodium tartrate BCC 0.12 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.7
pH 6.7
30.0 μl of 1,3-butanediol BCC (100 v/v)
87.0 μl of bis-tris propane chloride BCC pH 6 (1 M)
13.0 μl of bis-tris propane chloride BCC pH 10 (1 M)
120.0 μl of disodium tartrate BCC (1 M)
343.75 μl of polyethylene glycol 400 BCC (80 v/v)
406.25 μl of water (100 v/v)
C2
  • polyethylene glycol 400 BCC 27.5 v/v -
  • potassium sodium tartrate BCC 0.15 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.4
pH 6.4
95.6 μl of bis-tris propane chloride BCC pH 6 (1 M)
4.4 μl of bis-tris propane chloride BCC pH 10 (1 M)
343.75 μl of polyethylene glycol 400 BCC (80 v/v)
75.0 μl of potassium sodium tartrate BCC (2 M)
481.25 μl of water (100 v/v)
C3
  • polyethylene glycol 400 BCC 28 v/v -
  • potassium formate BCC 0.3 M -
  • bis-tris propane chloride BCC 0.1 M pH 7
pH 7
77.2 μl of bis-tris propane chloride BCC pH 6 (1 M)
22.8 μl of bis-tris propane chloride BCC pH 10 (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
300.0 μl of potassium formate BCC (1 M)
250.0 μl of water (100 v/v)
C4
  • polyethylene glycol 400 BCC 28 v/v -
  • ammonium dihydrogen phosphate BCC 0.3 M -
  • magnesium chloride BCC 0.01 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
120.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
10.0 μl of magnesium chloride BCC (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
55.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
420.0 μl of water (100 v/v)
C5
  • polyethylene glycol 400 BCC 28.5 v/v -
  • diammonium hydrogen citrate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
50.0 μl of diammonium hydrogen citrate BCC (2 M)
356.25 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
493.75 μl of water (100 v/v)
C6
  • polyethylene glycol 400 BCC 29 v/v -
  • diammonium hydrogen citrate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.8
pH 6.8
100.0 μl of diammonium hydrogen citrate BCC (2 M)
362.5 μl of polyethylene glycol 400 BCC (80 v/v)
12.37 μl of sodium MES BCC pH 5.2 (1 M)
87.63 μl of sodium MES BCC pH 7.2 (1 M)
437.5 μl of water (100 v/v)
C7
  • polyethylene glycol 400 BCC 29 v/v -
  • lithium sulfate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 4
pH 4
200.0 μl of lithium sulfate BCC (1 M)
362.5 μl of polyethylene glycol 400 BCC (80 v/v)
21.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
29.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
387.5 μl of water (100 v/v)
C8
  • polyethylene glycol 400 BCC 29.5 v/v -
  • 1,4-butanediol BCC 5 v/v -
  • sodium formate BCC 0.35 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
368.75 μl of polyethylene glycol 400 BCC (80 v/v)
87.5 μl of sodium formate BCC (4 M)
71.13 μl of tris chloride BCC pH 7 (1 M)
28.87 μl of tris chloride BCC pH 9 (1 M)
393.75 μl of water (100 v/v)
C9
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium malonate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
58.82 μl of sodium malonate BCC (3.4 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
466.18 μl of water (100 v/v)
C10
  • polyethylene glycol 400 BCC 30 v/v -
  • lithium sulfate BCC 0.185 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6.5
pH 6.5
185.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
96.76 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
3.24 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
340.0 μl of water (100 v/v)
C11
  • polyethylene glycol 400 BCC 30 v/v -
  • magnesium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
67.33 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.67 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
C12
  • polyethylene glycol 400 BCC 30 v/v -
  • lithium sulfate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
D1
  • polyethylene glycol 400 BCC 30 v/v -
  • ammonium acetate BCC 0.3 M -
  • glucose BCC 0.11 M -
  • bis-tris propane chloride BCC 0.1 M pH 7.5
pH 7.5
60.0 μl of ammonium acetate BCC (5 M)
61.67 μl of bis-tris propane chloride BCC pH 6 (1 M)
38.33 μl of bis-tris propane chloride BCC pH 10 (1 M)
110.0 μl of glucose BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
355.0 μl of water (100 v/v)
D2
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
425.0 μl of water (100 v/v)
D3
  • polyethylene glycol 400 BCC 30 v/v -
  • polypropylene glycol P400 BCC 2.5 v/v -
  • magnesium sulfate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
100.0 μl of magnesium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
41.67 μl of polypropylene glycol P400 BCC (60 v/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
383.33 μl of water (100 v/v)
D4
  • polyethylene glycol 400 BCC 30 v/v -
  • potassium nitrate BCC 0.4 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of potassium nitrate BCC (1 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
125.0 μl of water (100 v/v)
D5
  • polyethylene glycol 400 BCC 30 v/v -
  • lithium chloride BCC 0.4 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of lithium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.39 μl of tris chloride BCC pH 7 (1 M)
15.61 μl of tris chloride BCC pH 9 (1 M)
125.0 μl of water (100 v/v)
D6
  • polyethylene glycol 400 BCC 30 v/v -
  • magnesium sulfate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of magnesium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
425.0 μl of water (100 v/v)
D7
  • polyethylene glycol 400 BCC 30 v/v -
  • ammonium formate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.3
pH 6.3
100.0 μl of ammonium formate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.7 μl of sodium MES BCC pH 5.2 (1 M)
59.3 μl of sodium MES BCC pH 7.2 (1 M)
425.0 μl of water (100 v/v)
D8
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium thiocyanate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
475.0 μl of water (100 v/v)
D9
  • polyethylene glycol 400 BCC 30 v/v -
  • calcium chloride BCC 0.02 M -
  • sodium thiocyanate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
455.0 μl of water (100 v/v)
D10
  • polyethylene glycol 400 BCC 30 v/v -
  • calcium chloride BCC 0.02 M -
  • sodium thiocyanate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium thiocyanate BCC (2 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
455.0 μl of water (100 v/v)
D11
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • trehalose BCC 0.09 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
90.0 μl of trehalose BCC (1 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
335.0 μl of water (100 v/v)
D12
  • polyethylene glycol 400 BCC 30 v/v -
  • magnesium chloride BCC 0.1 M -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
100.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
13.88 μl of sodium MES BCC pH 5.2 (1 M)
36.12 μl of sodium MES BCC pH 7.2 (1 M)
475.0 μl of water (100 v/v)
E1
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
E2
  • hexammine cobalt(III) chloride BCC 0.005 M -
  • polyethylene glycol 400 BCC 31 v/v -
  • sodium malonate BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
50.0 μl of hexammine cobalt(III) chloride BCC (0.1 M)
387.5 μl of polyethylene glycol 400 BCC (80 v/v)
88.24 μl of sodium malonate BCC (3.4 M)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
374.26 μl of water (100 v/v)
E3
  • polyethylene glycol 400 BCC 31 v/v -
  • magnesium chloride BCC 0.35 M -
  • trisodium citrate BCC 0.15 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
350.0 μl of magnesium chloride BCC (1 M)
387.5 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
150.0 μl of trisodium citrate BCC (1 M)
12.5 μl of water (100 v/v)
E4
  • polyethylene glycol 400 BCC 32 v/v -
  • ammonium fluoride BCC 0.07 M -
  • polypropylene glycol P400 BCC 6 v/v -
  • sodium HEPES BCC 0.1 M pH 7.8
pH 7.8
70.0 μl of ammonium fluoride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of polypropylene glycol P400 BCC (60 v/v)
29.69 μl of sodium HEPES BCC pH 6.5 (1 M)
70.31 μl of sodium HEPES BCC pH 8.5 (1 M)
330.0 μl of water (100 v/v)
E5
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E6
  • polyethylene glycol 400 BCC 32 v/v -
  • calcium chloride BCC 0.02 M -
  • sodium thiocyanate BCC 0.1 M -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
13.88 μl of sodium MES BCC pH 5.2 (1 M)
36.12 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
480.0 μl of water (100 v/v)
E7
  • polyethylene glycol 400 BCC 32 v/v -
  • magnesium chloride BCC 0.13 M -
  • sodium chloride BCC 0.075 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
130.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
75.0 μl of sodium chloride BCC (1 M)
55.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
295.0 μl of water (100 v/v)
E8
  • polyethylene glycol 400 BCC 32.5 v/v -
  • 1,4-butanediol BCC 6 v/v -
  • sodium sulfate BCC 0.15 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.75
pH 6.75
60.0 μl of 1,4-butanediol BCC (100 v/v)
85.6 μl of bis-tris propane chloride BCC pH 6 (1 M)
14.4 μl of bis-tris propane chloride BCC pH 10 (1 M)
406.25 μl of polyethylene glycol 400 BCC (80 v/v)
125.0 μl of sodium sulfate BCC (1.2 M)
308.75 μl of water (100 v/v)
E9
  • polyethylene glycol 400 BCC 32.5 v/v -
  • ethylene glycol BCC 5 v/v -
  • potassium sodium tartrate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.2
pH 6.2
50.0 μl of ethylene glycol BCC (100 v/v)
406.25 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of potassium sodium tartrate BCC (2 M)
47.65 μl of sodium MES BCC pH 5.2 (1 M)
52.35 μl of sodium MES BCC pH 7.2 (1 M)
393.75 μl of water (100 v/v)
E10
  • polyethylene glycol 400 BCC 34 v/v -
  • ammonium sulfate BCC 0.12 M -
  • trisodium citrate BCC 0.09 M -
  • tris chloride BCC 0.1 M pH 8.7
pH 8.7
120.0 μl of ammonium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
10.92 μl of tris chloride BCC pH 7 (1 M)
89.08 μl of tris chloride BCC pH 9 (1 M)
90.0 μl of trisodium citrate BCC (1 M)
265.0 μl of water (100 v/v)
E11
  • polyethylene glycol 400 BCC 34 v/v -
  • lithium sulfate BCC 0.3 M -
  • dimethyl sulfoxide BCC 7.5 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
75.0 μl of dimethyl sulfoxide BCC (100 v/v)
300.0 μl of lithium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
E12
  • polyethylene glycol 400 BCC 35 v/v -
  • glycerol BCC 4 v/v -
  • trisodium citrate BCC 0.08 M -
  • tricine BCC 0.1 M pH 8.2
pH 8.2
50.0 μl of glycerol BCC (80 v/v)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
47.65 μl of tricine BCC pH 7.2 (1 M)
52.35 μl of tricine BCC pH 9.2 (1 M)
80.0 μl of trisodium citrate BCC (1 M)
332.5 μl of water (100 v/v)
F1
  • polyethylene glycol 400 BCC 35 v/v -
  • ammonium acetate BCC 0.37 M -
  • 1-propanol BCC 3 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
37.5 μl of 1-propanol BCC (80 v/v)
74.0 μl of ammonium acetate BCC (5 M)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
67.33 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.67 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
351.0 μl of water (100 v/v)
F2
  • polyethylene glycol 400 BCC 36 v/v -
  • ammonium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.2
pH 7.2
100.0 μl of ammonium chloride BCC (1 M)
450.0 μl of polyethylene glycol 400 BCC (80 v/v)
70.31 μl of sodium HEPES BCC pH 6.5 (1 M)
29.69 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
F3
  • polyethylene glycol 400 BCC 37.5 v/v -
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • diammonium tartrate BCC 0.18 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
90.0 μl of diammonium tartrate BCC (2 M)
468.75 μl of polyethylene glycol 400 BCC (80 v/v)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
301.25 μl of water (100 v/v)
F4
  • polyethylene glycol 400 BCC 38 v/v -
  • lithium chloride BCC 0.3 M -
  • strontium chloride BCC 0.03 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
300.0 μl of lithium chloride BCC (1 M)
475.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
30.0 μl of strontium chloride BCC (1 M)
95.0 μl of water (100 v/v)
F5
  • polyethylene glycol 400 BCC 39 v/v -
  • potassium chloride BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
487.5 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of potassium chloride BCC (1 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
312.5 μl of water (100 v/v)
F6
  • polyethylene glycol 400 BCC 39.8 v/v -
  • ammonium dihydrogen phosphate BCC 0.1 M -
  • bis-tris propane chloride BCC 0.1 M pH 7.2
pH 7.2
40.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
70.33 μl of bis-tris propane chloride BCC pH 6 (1 M)
29.67 μl of bis-tris propane chloride BCC pH 10 (1 M)
497.5 μl of polyethylene glycol 400 BCC (80 v/v)
362.5 μl of water (100 v/v)
F7
  • polyethylene glycol 400 BCC 40 v/v -
  • dimethyl sulfoxide BCC 4 v/v -
  • 1,7-heptanediol BCC 1 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
10.53 μl of 1,7-heptanediol BCC (95 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
349.47 μl of water (100 v/v)
F8
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium chloride BCC 1.6 M -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
320.0 μl of sodium chloride BCC (5 M)
13.88 μl of sodium MES BCC pH 5.2 (1 M)
36.12 μl of sodium MES BCC pH 7.2 (1 M)
130.0 μl of water (100 v/v)
F9
  • polyethylene glycol 400 BCC 40 v/v -
  • lithium chloride BCC 0.16 M -
  • strontium chloride BCC 0.004 M -
  • ADA BCC 0.1 M pH 6.5
pH 6.5
109.38 μl of ADA BCC pH 5.5 (0.5 M)
90.62 μl of ADA BCC pH 7.5 (0.5 M)
160.0 μl of lithium chloride BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
4.0 μl of strontium chloride BCC (1 M)
136.0 μl of water (100 v/v)
F10
  • polyethylene glycol 400 BCC 40 v/v -
  • lithium sulfate BCC 0.2 M -
  • ADA BCC 0.1 M pH 6.5
pH 6.5
109.38 μl of ADA BCC pH 5.5 (0.5 M)
90.62 μl of ADA BCC pH 7.5 (0.5 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of water (100 v/v)
F11
  • polyethylene glycol 400 BCC 42 v/v -
  • sodium acetate BCC 0.15 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
525.0 μl of polyethylene glycol 400 BCC (80 v/v)
150.0 μl of sodium acetate BCC (1 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
225.0 μl of water (100 v/v)
F12
  • polyethylene glycol dimethyl ether 500 BCC 25 v/v -
  • copper(II) chloride BCC 0.01 M -
  • ammonium formate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
200.0 μl of ammonium formate BCC (1 M)
10.0 μl of copper(II) chloride BCC (1 M)
312.5 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
377.5 μl of water (100 v/v)
G1
  • polyethylene glycol dimethyl ether 500 BCC 30 v/v -
  • cadmium chloride BCC 0.002 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
20.0 μl of cadmium chloride BCC (0.1 M)
375.0 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
505.0 μl of water (100 v/v)
G2
  • polyethylene glycol dimethyl ether 500 BCC 30 v/v -
  • ammonium sulfate BCC 0.1 M -
  • magnesium chloride BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 6
pH 6
100.0 μl of ammonium sulfate BCC (1 M)
100.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
90.5 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.5 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
225.0 μl of water (100 v/v)
G3
  • polyethylene glycol monomethyl ether 550 BCC 14 v/v -
  • lithium sulfate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of lithium sulfate BCC (1 M)
164.71 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
535.29 μl of water (100 v/v)
G4
  • polyethylene glycol monomethyl ether 550 BCC 15 v/v -
  • potassium sodium tartrate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
176.47 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of potassium sodium tartrate BCC (2 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
623.53 μl of water (100 v/v)
G5
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • sodium nitrate BCC 0.35 M -
  • sodium malonate-malonic acid BCC 0.05 M -
  • ADA BCC 0.05 M pH 6.25
pH 6.25
71.13 μl of ADA BCC pH 5.5 (0.5 M)
28.87 μl of ADA BCC pH 7.5 (0.5 M)
294.12 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
14.71 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
350.0 μl of sodium nitrate BCC (1 M)
241.17 μl of water (100 v/v)
G6
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
  • lithium sulfate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of lithium sulfate BCC (1 M)
329.41 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
470.59 μl of water (100 v/v)
G7
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
  • ammonium sulfate BCC 0.55 M -
  • ADA BCC 0.05 M pH 7
pH 7
21.23 μl of ADA BCC pH 5.5 (0.5 M)
78.77 μl of ADA BCC pH 7.5 (0.5 M)
550.0 μl of ammonium sulfate BCC (1 M)
329.41 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.59 μl of water (100 v/v)
G8
  • polyethylene glycol 600 BCC 25 w/v -
  • ADA BCC 0.1 M pH 7
pH 7
42.46 μl of ADA BCC pH 5.5 (0.5 M)
157.54 μl of ADA BCC pH 7.5 (0.5 M)
500.0 μl of polyethylene glycol 600 BCC (50 w/v)
300.0 μl of water (100 v/v)
G9
  • polyethylene glycol 1500 BCC 22.5 w/v -
  • sodium acetate BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
450.0 μl of polyethylene glycol 1500 BCC (50 w/v)
300.0 μl of sodium acetate BCC (1 M)
89.96 μl of sodium MES BCC pH 5.2 (1 M)
10.04 μl of sodium MES BCC pH 7.2 (1 M)
150.0 μl of water (100 v/v)
G10
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
240.0 μl of polyethylene glycol 3350 BCC (50 w/v)
37.93 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.07 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
58.82 μl of sodium malonate BCC (3.4 M)
601.18 μl of water (100 v/v)
G11
  • polyethylene glycol 3350 BCC 25 w/v -
  • lithium sulfate BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of water (100 v/v)
G12
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H1
  • polyethylene glycol 4000 BCC 10 w/v -
  • potassium chloride BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
200.0 μl of potassium chloride BCC (1 M)
37.93 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.07 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of water (100 v/v)
H2
  • polyethylene glycol 6000 BCC 20 w/v -
  • sodium bicine BCC 0.1 M pH 9
pH 9
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
12.37 μl of sodium bicine BCC pH 7.4 (1 M)
87.63 μl of sodium bicine BCC pH 9.4 (1 M)
500.0 μl of water (100 v/v)
H3
  • polyethylene glycol 8000 BCC 30 w/v -
  • ammonium sulfate BCC 0.2 M -
NA
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of water (100 v/v)
H4
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 10 w/v -
  • ammonium chloride BCC 0.1 M -
  • calcium chloride BCC 0.01 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of ammonium chloride BCC (1 M)
10.0 μl of calcium chloride BCC (1 M)
200.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
590.0 μl of water (100 v/v)
H5
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 20 w/v -
  • potassium formate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
100.0 μl of potassium formate BCC (1 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
H6
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • lithium nitrate BCC 0.1 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of lithium nitrate BCC (1 M)
20.0 μl of sodium chloride BCC (5 M)
81.43 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.57 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
740.0 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • magnesium acetate BCC 0.06 M -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
24.0 μl of magnesium acetate BCC (2.5 M)
20.0 μl of sodium chloride BCC (5 M)
81.43 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.57 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
806.0 μl of water (100 v/v)
H8
  • potassium nitrate BCC 0.4 M -
  • 2-methyl-2,4-pentanediol BCC 8 v/v -
  • tripotassium citrate BCC 0.1 M -
  • ADA BCC 0.1 M pH 6.7
pH 6.7
80.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
81.24 μl of ADA BCC pH 5.5 (0.5 M)
118.76 μl of ADA BCC pH 7.5 (0.5 M)
400.0 μl of potassium nitrate BCC (1 M)
100.0 μl of tripotassium citrate BCC (1 M)
220.0 μl of water (100 v/v)
H9
  • trehalose BCC 0.3 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1 M pH 5.1
pH 5.1
328.69 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
4.64 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
300.0 μl of trehalose BCC (1 M)
366.67 μl of water (100 v/v)
H10
  • sodium chloride BCC 1.5 M -
  • sodium acetate-acetic acid BCC 0.075 M pH 4.6
pH 4.6
28.45 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
46.55 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
300.0 μl of sodium chloride BCC (5 M)
625.0 μl of water (100 v/v)
H11
  • sodium acetate BCC 1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
333.33 μl of sodium acetate BCC (3 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
566.67 μl of water (100 v/v)
H12
  • nickel(II) chloride BCC 0.01 M -
  • lithium sulfate BCC 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of lithium sulfate BCC (2.5 M)
10.0 μl of nickel(II) chloride BCC (1 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)