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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
LCP_C3_right
BCC Core Screens / LCP_C3_right
Created 2025-03-06.
The right-hand half (columns 7-12) of LCP screen. This is purely for operational purposes in the BCC lab.
Export
LCP_C3_right
Barcodes
LCP_C3_right
Screen Structure
LCP_C3_right
Well Count | 96 |
---|---|
Number of distinct ingredients | 48 |
Internal diversity (score mean dist) | 0.876 |
Screen Composition
ingredient type | LCP_C3_right |
---|---|
Buffer | 54 |
Cryoprotectant | 3 |
Organic (non-volatile) | 8 |
Organic (volatile) | 8 |
Polymer | 44 |
Salt | 63 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 | NA | ||
A2 | NA | ||
A3 | NA | ||
A4 | NA | ||
A5 | NA | ||
A6 | NA | ||
A7 |
| pH 6 | 61 μl of sodium MES BCC pH 5.2 (1 M) 9 μl of jeffamine M-600 BCC pH 7 (50 v/v) 162 μl of polyethylene glycol 400 BCC (80 v/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 85 μl of potassium sodium tartrate BCC (2 M) 643 μl of water (100 v/v) |
A8 |
| pH 7.5 | 15 μl of tris chloride BCC pH 9 (1 M) 175 μl of polyethylene glycol 400 BCC (80 v/v) 84 μl of tris chloride BCC pH 7 (1 M) 60 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 665 μl of water (100 v/v) |
A9 |
| pH 7.5 | 15 μl of tris chloride BCC pH 9 (1 M) 225 μl of polyethylene glycol 400 BCC (80 v/v) 100 μl of trisodium citrate BCC (1 M) 84 μl of tris chloride BCC pH 7 (1 M) 575 μl of water (100 v/v) |
A10 |
| pH 7 | 40 μl of sodium HEPES BCC pH 6.5 (1 M) 200 μl of potassium sulfate BCC (0.5 M) 9 μl of sodium HEPES BCC pH 8.5 (1 M) 225 μl of polyethylene glycol 400 BCC (80 v/v) 525 μl of water (100 v/v) |
A11 |
| pH 5 | 100 μl of sodium chloride BCC (1 M) 50 μl of lithium sulfate BCC (1 M) 231 μl of polyethylene glycol 400 BCC (80 v/v) 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 518 μl of water (100 v/v) |
A12 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 140 μl of ammonium dihydrogen phosphate BCC (2.5 M) 243 μl of polyethylene glycol 400 BCC (80 v/v) 516 μl of water (100 v/v) |
B1 | NA | ||
B2 | NA | ||
B3 | NA | ||
B4 | NA | ||
B5 | NA | ||
B6 | NA | ||
B7 |
| pH 6.7 | 16 μl of sodium MES BCC pH 5.2 (1 M) 200 μl of lithium sulfate BCC (1 M) 40 μl of dimethyl sulfoxide BCC (100 v/v) 325 μl of polyethylene glycol 400 BCC (80 v/v) 35 μl of 1,4-butanediol BCC (100 v/v) 83 μl of sodium MES BCC pH 7.2 (1 M) 300 μl of water (100 v/v) |
B8 |
| pH 6 | 5 μl of strontium chloride BCC (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 88 μl of sodium malonate BCC (3.4 M) 325 μl of polyethylene glycol 400 BCC (80 v/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 481 μl of water (100 v/v) |
B9 |
| pH 8 | 300 μl of ammonium sulfate BCC (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 325 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 275 μl of water (100 v/v) |
B10 |
| pH 5 | 25 μl of sodium thiocyanate BCC (2 M) 40 μl of 2,5-hexanediol BCC (50 v/v) 331 μl of polyethylene glycol 400 BCC (80 v/v) 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 503 μl of water (100 v/v) |
B11 |
| pH 7.8 | 55 μl of sodium formate BCC (4 M) 28 μl of tris chloride BCC pH 9 (1 M) 337 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of 1,4-butanediol BCC (100 v/v) 71 μl of tris chloride BCC pH 7 (1 M) 457 μl of water (100 v/v) |
B12 |
| pH 6.9 | 86 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of jeffamine M-600 BCC pH 7 (50 v/v) 13 μl of sodium HEPES BCC pH 8.5 (1 M) 337 μl of polyethylene glycol 400 BCC (80 v/v) 150 μl of ammonium fluoride BCC (1 M) 362 μl of water (100 v/v) |
C1 | NA | ||
C2 | NA | ||
C3 | NA | ||
C4 | NA | ||
C5 | NA | ||
C6 | NA | ||
C7 |
| pH 4 | 200 μl of lithium sulfate BCC (1 M) 362 μl of polyethylene glycol 400 BCC (80 v/v) 21 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 29 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 387 μl of water (100 v/v) |
C8 |
| pH 7.8 | 87 μl of sodium formate BCC (4 M) 28 μl of tris chloride BCC pH 9 (1 M) 368 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of 1,4-butanediol BCC (100 v/v) 71 μl of tris chloride BCC pH 7 (1 M) 393 μl of water (100 v/v) |
C9 |
| pH 8 | 58 μl of sodium malonate BCC (3.4 M) 45 μl of tris chloride BCC pH 9 (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 466 μl of water (100 v/v) |
C10 |
| pH 6.5 | 185 μl of lithium sulfate BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 96 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 4 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 340 μl of water (100 v/v) |
C11 |
| pH 5 | 200 μl of magnesium chloride BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 68 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 325 μl of water (100 v/v) |
C12 |
| pH 5.5 | 200 μl of lithium sulfate BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 325 μl of water (100 v/v) |
D1 | NA | ||
D2 | NA | ||
D3 | NA | ||
D4 | NA | ||
D5 | NA | ||
D6 | NA | ||
D7 |
| pH 6.3 | 40 μl of sodium MES BCC pH 5.2 (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 59 μl of sodium MES BCC pH 7.2 (1 M) 100 μl of ammonium formate BCC (1 M) 425 μl of water (100 v/v) |
D8 |
| pH 7 | 50 μl of sodium thiocyanate BCC (2 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 475 μl of water (100 v/v) |
D9 |
| pH 6.5 | 50 μl of sodium thiocyanate BCC (2 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 20 μl of calcium chloride BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 455 μl of water (100 v/v) |
D10 |
| pH 8 | 50 μl of sodium thiocyanate BCC (2 M) 45 μl of tris chloride BCC pH 9 (1 M) 20 μl of calcium chloride BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 455 μl of water (100 v/v) |
D11 |
| pH 5.5 | 100 μl of sodium chloride BCC (1 M) 90 μl of trehalose BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 335 μl of water (100 v/v) |
D12 |
| pH 6.5 | 13 μl of sodium MES BCC pH 5.2 (1 M) 100 μl of magnesium chloride BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 36 μl of sodium MES BCC pH 7.2 (1 M) 475 μl of water (100 v/v) |
E1 | NA | ||
E2 | NA | ||
E3 | NA | ||
E4 | NA | ||
E5 | NA | ||
E6 | NA | ||
E7 |
| pH 4.5 | 75 μl of sodium chloride BCC (1 M) 130 μl of magnesium chloride BCC (1 M) 400 μl of polyethylene glycol 400 BCC (80 v/v) 56 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 44 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 295 μl of water (100 v/v) |
E8 |
| pH 6.8 | 125 μl of sodium sulfate BCC (1.2 M) 14 μl of bis-tris propane chloride BCC pH 10 (1 M) 86 μl of bis-tris propane chloride BCC pH 6 (1 M) 406 μl of polyethylene glycol 400 BCC (80 v/v) 60 μl of 1,4-butanediol BCC (100 v/v) 308 μl of water (100 v/v) |
E9 |
| pH 6.2 | 47 μl of sodium MES BCC pH 5.2 (1 M) 406 μl of polyethylene glycol 400 BCC (80 v/v) 52 μl of sodium MES BCC pH 7.2 (1 M) 50 μl of ethylene glycol BCC (100 v/v) 50 μl of potassium sodium tartrate BCC (2 M) 393 μl of water (100 v/v) |
E10 |
| pH 8.7 | 120 μl of ammonium sulfate BCC (1 M) 89 μl of tris chloride BCC pH 9 (1 M) 425 μl of polyethylene glycol 400 BCC (80 v/v) 90 μl of trisodium citrate BCC (1 M) 10 μl of tris chloride BCC pH 7 (1 M) 265 μl of water (100 v/v) |
E11 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of lithium sulfate BCC (1 M) 75 μl of dimethyl sulfoxide BCC (100 v/v) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 425 μl of polyethylene glycol 400 BCC (80 v/v) 100 μl of water (100 v/v) |
E12 |
| pH 8.2 | 52 μl of tricine BCC pH 9.2 (1 M) 50 μl of glycerol BCC (80 v/v) 437 μl of polyethylene glycol 400 BCC (80 v/v) 47 μl of tricine BCC pH 7.2 (1 M) 80 μl of trisodium citrate BCC (1 M) 332 μl of water (100 v/v) |
F1 | NA | ||
F2 | NA | ||
F3 | NA | ||
F4 | NA | ||
F5 | NA | ||
F6 | NA | ||
F7 |
| pH 8 | 10 μl of 1,7-heptanediol BCC (95 v/v) 40 μl of dimethyl sulfoxide BCC (100 v/v) 45 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 400 BCC (80 v/v) 54 μl of tris chloride BCC pH 7 (1 M) 349 μl of water (100 v/v) |
F8 |
| pH 6.5 | 13 μl of sodium MES BCC pH 5.2 (1 M) 320 μl of sodium chloride BCC (5 M) 500 μl of polyethylene glycol 400 BCC (80 v/v) 36 μl of sodium MES BCC pH 7.2 (1 M) 130 μl of water (100 v/v) |
F9 |
| pH 6.5 | 4 μl of strontium chloride BCC (1 M) 500 μl of polyethylene glycol 400 BCC (80 v/v) 109 μl of ADA BCC pH 5.5 (0.5 M) 160 μl of lithium chloride BCC (1 M) 90 μl of ADA BCC pH 7.5 (0.5 M) 136 μl of water (100 v/v) |
F10 |
| pH 6.5 | 200 μl of lithium sulfate BCC (1 M) 500 μl of polyethylene glycol 400 BCC (80 v/v) 109 μl of ADA BCC pH 5.5 (0.5 M) 90 μl of ADA BCC pH 7.5 (0.5 M) 100 μl of water (100 v/v) |
F11 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 150 μl of sodium acetate BCC (1 M) 525 μl of polyethylene glycol 400 BCC (80 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 225 μl of water (100 v/v) |
F12 |
| pH 6 | 61 μl of sodium MES BCC pH 5.2 (1 M) 10 μl of copper(II) chloride BCC (1 M) 312 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v) 38 μl of sodium MES BCC pH 7.2 (1 M) 200 μl of ammonium formate BCC (1 M) 377 μl of water (100 v/v) |
G1 | NA | ||
G2 | NA | ||
G3 | NA | ||
G4 | NA | ||
G5 | NA | ||
G6 | NA | ||
G7 |
| pH 7 | 329 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 550 μl of ammonium sulfate BCC (1 M) 21 μl of ADA BCC pH 5.5 (0.5 M) 78 μl of ADA BCC pH 7.5 (0.5 M) 20 μl of water (100 v/v) |
G8 |
| pH 7 | 500 μl of polyethylene glycol 600 BCC (50 w/v) 42 μl of ADA BCC pH 5.5 (0.5 M) 157 μl of ADA BCC pH 7.5 (0.5 M) 300 μl of water (100 v/v) |
G9 |
| pH 5.5 | 90 μl of sodium MES BCC pH 5.2 (1 M) 300 μl of sodium acetate BCC (1 M) 450 μl of polyethylene glycol 1500 BCC (50 w/v) 10 μl of sodium MES BCC pH 7.2 (1 M) 150 μl of water (100 v/v) |
G10 |
| pH 4.6 | 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 58 μl of sodium malonate BCC (3.4 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 240 μl of polyethylene glycol 3350 BCC (50 w/v) 601 μl of water (100 v/v) |
G11 |
| pH 5.5 | 200 μl of lithium sulfate BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of water (100 v/v) |
G12 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 400 μl of water (100 v/v) |
H1 | NA | ||
H2 | NA | ||
H3 | NA | ||
H4 | NA | ||
H5 | NA | ||
H6 | NA | ||
H7 |
| pH 5.6 | 20 μl of sodium chloride BCC (5 M) 82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 24 μl of magnesium acetate BCC (2.5 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 806 μl of water (100 v/v) |
H8 |
| pH 6.7 | 100 μl of tripotassium citrate BCC (1 M) 400 μl of potassium nitrate BCC (1 M) 81 μl of ADA BCC pH 5.5 (0.5 M) 118 μl of ADA BCC pH 7.5 (0.5 M) 80 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 220 μl of water (100 v/v) |
H9 |
| pH 5.1 | 326 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 300 μl of trehalose BCC (1 M) 6 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 366 μl of water (100 v/v) |
H10 |
| pH 4.6 | 300 μl of sodium chloride BCC (5 M) 28 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 46 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 625 μl of water (100 v/v) |
H11 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 333 μl of sodium acetate BCC (3 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 566 μl of water (100 v/v) |
H12 |
| pH 8.5 | 10 μl of nickel(II) chloride BCC (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 400 μl of lithium sulfate BCC (2.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 490 μl of water (100 v/v) |