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Supplemental Information

Screen Description

BCC Core Screens / LCP_C3_right
Created 2025-03-06.
The right-hand half (columns 7-12) of LCP screen. This is purely for operational purposes in the BCC lab.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 48
Internal diversity (score mean dist) 0.88

Screen Composition

2025-12-05T23:38:04.636682 image/svg+xml Matplotlib v3.10.7, https://matplotlib.org/
ingredient type LCP_C3_right
Buffer 54
Cryoprotectant 3
Organic (non-volatile) 8
Organic (volatile) 8
Polymer 44
Salt 63

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
LCP_C3_right Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1NA
A2NA
A3NA
A4NA
A5NA
A6NA
A7
  • jeffamine M-600 BCC 0.45 v/v -
  • polyethylene glycol 400 BCC 13 v/v -
  • potassium sodium tartrate BCC 0.17 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
9.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
162.5 μl of polyethylene glycol 400 BCC (80 v/v)
85.0 μl of potassium sodium tartrate BCC (2 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
643.5 μl of water (100 v/v)
A8
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
  • polyethylene glycol 400 BCC 14 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
175.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
665.0 μl of water (100 v/v)
A9
  • polyethylene glycol 400 BCC 18 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • trisodium citrate BCC 0.1 M -
pH 7.5
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
84.4 μl of tris chloride BCC pH 7 (1 M)
15.6 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of trisodium citrate BCC (1 M)
575.0 μl of water (100 v/v)
A10
  • polyethylene glycol 400 BCC 18 v/v -
  • potassium sulfate BCC 0.1 M -
  • sodium HEPES BCC 0.05 M pH 7
pH 7
225.0 μl of polyethylene glycol 400 BCC (80 v/v)
200.0 μl of potassium sulfate BCC (0.5 M)
40.9 μl of sodium HEPES BCC pH 6.5 (1 M)
9.1 μl of sodium HEPES BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
A11
  • lithium sulfate BCC 0.05 M -
  • polyethylene glycol 400 BCC 18.5 v/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
50.0 μl of lithium sulfate BCC (1 M)
231.2 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
518.8 μl of water (100 v/v)
A12
  • ammonium dihydrogen phosphate BCC 0.35 M -
  • polyethylene glycol 400 BCC 19.5 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
140.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
243.8 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
516.2 μl of water (100 v/v)
B1NA
B2NA
B3NA
B4NA
B5NA
B6NA
B7
  • 1,4-butanediol BCC 3.5 v/v -
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium MES BCC 0.1 M pH 6.7
  • dimethyl sulfoxide BCC 4 v/v -
pH 6.7
35.0 μl of 1,4-butanediol BCC (100 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
200.0 μl of lithium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
16.9 μl of sodium MES BCC pH 5.2 (1 M)
83.1 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
B8
  • polyethylene glycol 400 BCC 26 v/v -
  • sodium MES BCC 0.1 M pH 6
  • strontium chloride BCC 0.005 M -
  • sodium malonate BCC 0.3 M -
pH 6
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
88.2 μl of sodium malonate BCC (3.4 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
5.0 μl of strontium chloride BCC (1 M)
481.8 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 0.3 M -
  • polyethylene glycol 400 BCC 26 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of ammonium sulfate BCC (1 M)
325.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
275.0 μl of water (100 v/v)
B10
  • polyethylene glycol 400 BCC 26.5 v/v -
  • sodium thiocyanate BCC 0.05 M -
  • 2,5-hexanediol BCC 2 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
40.0 μl of 2,5-hexanediol BCC (50 v/v)
331.2 μl of polyethylene glycol 400 BCC (80 v/v)
25.0 μl of sodium thiocyanate BCC (2 M)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
503.8 μl of water (100 v/v)
B11
  • 1,4-butanediol BCC 5 v/v -
  • polyethylene glycol 400 BCC 27 v/v -
  • sodium formate BCC 0.22 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
55.0 μl of sodium formate BCC (4 M)
71.1 μl of tris chloride BCC pH 7 (1 M)
28.9 μl of tris chloride BCC pH 9 (1 M)
457.5 μl of water (100 v/v)
B12
  • ammonium fluoride BCC 0.15 M -
  • jeffamine M-600 BCC 2.5 v/v -
  • polyethylene glycol 400 BCC 27 v/v -
  • sodium HEPES BCC 0.1 M pH 6.9
pH 6.9
150.0 μl of ammonium fluoride BCC (1 M)
50.0 μl of jeffamine M-600 BCC pH 7 (50 v/v)
337.5 μl of polyethylene glycol 400 BCC (80 v/v)
86.6 μl of sodium HEPES BCC pH 6.5 (1 M)
13.4 μl of sodium HEPES BCC pH 8.5 (1 M)
362.5 μl of water (100 v/v)
C1NA
C2NA
C3NA
C4NA
C5NA
C6NA
C7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 29 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 4
pH 4
200.0 μl of lithium sulfate BCC (1 M)
362.5 μl of polyethylene glycol 400 BCC (80 v/v)
21.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
29.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
387.5 μl of water (100 v/v)
C8
  • 1,4-butanediol BCC 5 v/v -
  • polyethylene glycol 400 BCC 29.5 v/v -
  • sodium formate BCC 0.35 M -
  • tris chloride BCC 0.1 M pH 7.75
pH 7.75
50.0 μl of 1,4-butanediol BCC (100 v/v)
368.8 μl of polyethylene glycol 400 BCC (80 v/v)
87.5 μl of sodium formate BCC (4 M)
71.1 μl of tris chloride BCC pH 7 (1 M)
28.9 μl of tris chloride BCC pH 9 (1 M)
393.8 μl of water (100 v/v)
C9
  • polyethylene glycol 400 BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • sodium malonate BCC 0.2 M -
pH 8
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
58.8 μl of sodium malonate BCC (3.4 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
466.2 μl of water (100 v/v)
C10
  • lithium sulfate BCC 0.185 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 6.5
pH 6.5
185.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
96.8 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
3.2 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
340.0 μl of water (100 v/v)
C11
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5
pH 5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
67.3 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
32.7 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
C12
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of lithium sulfate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
325.0 μl of water (100 v/v)
D1NA
D2NA
D3NA
D4NA
D5NA
D6NA
D7
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.3
  • ammonium formate BCC 0.1 M -
pH 6.3
100.0 μl of ammonium formate BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
40.7 μl of sodium MES BCC pH 5.2 (1 M)
59.3 μl of sodium MES BCC pH 7.2 (1 M)
425.0 μl of water (100 v/v)
D8
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7
  • sodium thiocyanate BCC 0.1 M -
pH 7
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
475.0 μl of water (100 v/v)
D9
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • sodium thiocyanate BCC 0.1 M -
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium thiocyanate BCC (2 M)
455.0 μl of water (100 v/v)
D10
  • calcium chloride BCC 0.02 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium thiocyanate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20.0 μl of calcium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium thiocyanate BCC (2 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
455.0 μl of water (100 v/v)
D11
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium chloride BCC 0.1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • trehalose BCC 0.09 M -
pH 5.5
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of sodium chloride BCC (1 M)
90.0 μl of trehalose BCC (1 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
335.0 μl of water (100 v/v)
D12
  • magnesium chloride BCC 0.1 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
100.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
475.0 μl of water (100 v/v)
E1NA
E2NA
E3NA
E4NA
E5NA
E6NA
E7
  • magnesium chloride BCC 0.13 M -
  • polyethylene glycol 400 BCC 32 v/v -
  • sodium chloride BCC 0.075 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 4.5
pH 4.5
130.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 400 BCC (80 v/v)
75.0 μl of sodium chloride BCC (1 M)
55.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
295.0 μl of water (100 v/v)
E8
  • 1,4-butanediol BCC 6 v/v -
  • polyethylene glycol 400 BCC 32.5 v/v -
  • sodium sulfate BCC 0.15 M -
  • bis-tris propane chloride BCC 0.1 M pH 6.75
pH 6.75
60.0 μl of 1,4-butanediol BCC (100 v/v)
85.6 μl of bis-tris propane chloride BCC pH 6 (1 M)
14.4 μl of bis-tris propane chloride BCC pH 10 (1 M)
406.2 μl of polyethylene glycol 400 BCC (80 v/v)
125.0 μl of sodium sulfate BCC (1.2 M)
308.8 μl of water (100 v/v)
E9
  • ethylene glycol BCC 5 v/v -
  • polyethylene glycol 400 BCC 32.5 v/v -
  • potassium sodium tartrate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.2
pH 6.2
50.0 μl of ethylene glycol BCC (100 v/v)
406.2 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of potassium sodium tartrate BCC (2 M)
47.6 μl of sodium MES BCC pH 5.2 (1 M)
52.4 μl of sodium MES BCC pH 7.2 (1 M)
393.8 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 0.12 M -
  • polyethylene glycol 400 BCC 34 v/v -
  • tris chloride BCC 0.1 M pH 8.7
  • trisodium citrate BCC 0.09 M -
pH 8.7
120.0 μl of ammonium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
10.9 μl of tris chloride BCC pH 7 (1 M)
89.1 μl of tris chloride BCC pH 9 (1 M)
90.0 μl of trisodium citrate BCC (1 M)
265.0 μl of water (100 v/v)
E11
  • lithium sulfate BCC 0.3 M -
  • polyethylene glycol 400 BCC 34 v/v -
  • sodium HEPES BCC 0.1 M pH 7
  • dimethyl sulfoxide BCC 7.5 v/v -
pH 7
75.0 μl of dimethyl sulfoxide BCC (100 v/v)
300.0 μl of lithium sulfate BCC (1 M)
425.0 μl of polyethylene glycol 400 BCC (80 v/v)
81.8 μl of sodium HEPES BCC pH 6.5 (1 M)
18.2 μl of sodium HEPES BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
E12
  • glycerol BCC 4 v/v -
  • polyethylene glycol 400 BCC 35 v/v -
  • tricine BCC 0.1 M pH 8.2
  • trisodium citrate BCC 0.08 M -
pH 8.2
50.0 μl of glycerol BCC (80 v/v)
437.5 μl of polyethylene glycol 400 BCC (80 v/v)
47.6 μl of tricine BCC pH 7.2 (1 M)
52.4 μl of tricine BCC pH 9.2 (1 M)
80.0 μl of trisodium citrate BCC (1 M)
332.5 μl of water (100 v/v)
F1NA
F2NA
F3NA
F4NA
F5NA
F6NA
F7
  • polyethylene glycol 400 BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
  • dimethyl sulfoxide BCC 4 v/v -
  • 1,7-heptanediol BCC 1 v/v -
pH 8
10.5 μl of 1,7-heptanediol BCC (95 v/v)
40.0 μl of dimethyl sulfoxide BCC (100 v/v)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
349.5 μl of water (100 v/v)
F8
  • polyethylene glycol 400 BCC 40 v/v -
  • sodium chloride BCC 1.6 M -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
320.0 μl of sodium chloride BCC (5 M)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
130.0 μl of water (100 v/v)
F9
  • ADA BCC 0.1 M pH 6.5
  • lithium chloride BCC 0.16 M -
  • polyethylene glycol 400 BCC 40 v/v -
  • strontium chloride BCC 0.004 M -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
160.0 μl of lithium chloride BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
4.0 μl of strontium chloride BCC (1 M)
136.0 μl of water (100 v/v)
F10
  • ADA BCC 0.1 M pH 6.5
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 400 BCC 40 v/v -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 400 BCC (80 v/v)
100.0 μl of water (100 v/v)
F11
  • polyethylene glycol 400 BCC 42 v/v -
  • sodium acetate BCC 0.15 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
525.0 μl of polyethylene glycol 400 BCC (80 v/v)
150.0 μl of sodium acetate BCC (1 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
225.0 μl of water (100 v/v)
F12
  • sodium MES BCC 0.1 M pH 6
  • copper(II) chloride BCC 0.01 M -
  • ammonium formate BCC 0.2 M -
  • polyethylene glycol dimethyl ether 500 BCC 25 v/v -
pH 6
200.0 μl of ammonium formate BCC (1 M)
10.0 μl of copper(II) chloride BCC (1 M)
312.5 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
377.5 μl of water (100 v/v)
G1NA
G2NA
G3NA
G4NA
G5NA
G6NA
G7
  • ADA BCC 0.05 M pH 7
  • ammonium sulfate BCC 0.55 M -
  • polyethylene glycol monomethyl ether 550 BCC 28 v/v -
pH 7
21.2 μl of ADA BCC pH 5.5 (0.5 M)
78.8 μl of ADA BCC pH 7.5 (0.5 M)
550.0 μl of ammonium sulfate BCC (1 M)
329.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.6 μl of water (100 v/v)
G8
  • ADA BCC 0.1 M pH 7
  • polyethylene glycol 600 BCC 25 w/v -
pH 7
42.5 μl of ADA BCC pH 5.5 (0.5 M)
157.5 μl of ADA BCC pH 7.5 (0.5 M)
500.0 μl of polyethylene glycol 600 BCC (50 w/v)
300.0 μl of water (100 v/v)
G9
  • polyethylene glycol 1500 BCC 22.5 w/v -
  • sodium acetate BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 5.5
pH 5.5
450.0 μl of polyethylene glycol 1500 BCC (50 w/v)
300.0 μl of sodium acetate BCC (1 M)
90.0 μl of sodium MES BCC pH 5.2 (1 M)
10.0 μl of sodium MES BCC pH 7.2 (1 M)
150.0 μl of water (100 v/v)
G10
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium malonate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
240.0 μl of polyethylene glycol 3350 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
58.8 μl of sodium malonate BCC (3.4 M)
601.2 μl of water (100 v/v)
G11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of water (100 v/v)
G12
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H1NA
H2NA
H3NA
H4NA
H5NA
H6NA
H7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • magnesium acetate BCC 0.06 M -
  • sodium chloride BCC 0.1 M -
pH 5.6
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
24.0 μl of magnesium acetate BCC (2.5 M)
20.0 μl of sodium chloride BCC (5 M)
81.4 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.6 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
806.0 μl of water (100 v/v)
H8
  • ADA BCC 0.1 M pH 6.7
  • 2-methyl-2,4-pentanediol BCC 8 v/v -
  • tripotassium citrate BCC 0.1 M -
  • potassium nitrate BCC 0.4 M -
pH 6.7
80.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
81.2 μl of ADA BCC pH 5.5 (0.5 M)
118.8 μl of ADA BCC pH 7.5 (0.5 M)
400.0 μl of potassium nitrate BCC (1 M)
100.0 μl of tripotassium citrate BCC (1 M)
220.0 μl of water (100 v/v)
H9
  • trehalose BCC 0.3 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 1 M pH 5.1
pH 5.1
328.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M)
4.6 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M)
300.0 μl of trehalose BCC (1 M)
366.7 μl of water (100 v/v)
H10
  • sodium acetate-acetic acid BCC 0.075 M pH 4.6
  • sodium chloride BCC 1.5 M -
pH 4.6
28.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
46.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
300.0 μl of sodium chloride BCC (5 M)
625.0 μl of water (100 v/v)
H11
  • sodium MES BCC 0.1 M pH 6.5
  • sodium acetate BCC 1 M -
pH 6.5
333.3 μl of sodium acetate BCC (3 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
566.7 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • lithium sulfate BCC 1 M -
  • nickel(II) chloride BCC 0.01 M -
pH 8.5
400.0 μl of lithium sulfate BCC (2.5 M)
10.0 μl of nickel(II) chloride BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)