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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
LCP_C3_right
BCC Core Screens / LCP_C3_right
Created 2025-03-06.
The right-hand half (columns 7-12) of LCP screen. This is purely for operational purposes in the BCC lab.
Export
LCP_C3_right
Barcodes
LCP_C3_right
No barcodes found.
Screen Structure
LCP_C3_right
Well Count | 96 |
---|---|
Number of distinct ingredients | 48 |
Internal diversity (score mean dist) | 0.876 |
Screen Composition
ingredient type | LCP_C3_right |
---|---|
Buffer | 54 |
Cryoprotectant | 3 |
Organic (non-volatile) | 8 |
Organic (volatile) | 8 |
Polymer | 44 |
Salt | 63 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 | NA | 1000.0 μl of water (100 v/v) | |
A2 | NA | 1000.0 μl of water (100 v/v) | |
A3 | NA | 1000.0 μl of water (100 v/v) | |
A4 | NA | 1000.0 μl of water (100 v/v) | |
A5 | NA | 1000.0 μl of water (100 v/v) | |
A6 | NA | 1000.0 μl of water (100 v/v) | |
A7 |
| pH 6 | 9.0 μl of jeffamine M-600 BCC pH 7 (50 v/v) 162.5 μl of polyethylene glycol 400 BCC (80 v/v) 85.0 μl of potassium sodium tartrate BCC (2 M) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 643.5 μl of water (100 v/v) |
A8 |
| pH 7.5 | 60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 175.0 μl of polyethylene glycol 400 BCC (80 v/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 665.0 μl of water (100 v/v) |
A9 |
| pH 7.5 | 225.0 μl of polyethylene glycol 400 BCC (80 v/v) 84.4 μl of tris chloride BCC pH 7 (1 M) 15.6 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of trisodium citrate BCC (1 M) 575.0 μl of water (100 v/v) |
A10 |
| pH 7 | 225.0 μl of polyethylene glycol 400 BCC (80 v/v) 200.0 μl of potassium sulfate BCC (0.5 M) 40.9 μl of sodium HEPES BCC pH 6.5 (1 M) 9.1 μl of sodium HEPES BCC pH 8.5 (1 M) 525.0 μl of water (100 v/v) |
A11 |
| pH 5 | 50.0 μl of lithium sulfate BCC (1 M) 231.2 μl of polyethylene glycol 400 BCC (80 v/v) 100.0 μl of sodium chloride BCC (1 M) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 518.8 μl of water (100 v/v) |
A12 |
| pH 7 | 140.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 243.8 μl of polyethylene glycol 400 BCC (80 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 516.2 μl of water (100 v/v) |
B1 | NA | 1000.0 μl of water (100 v/v) | |
B2 | NA | 1000.0 μl of water (100 v/v) | |
B3 | NA | 1000.0 μl of water (100 v/v) | |
B4 | NA | 1000.0 μl of water (100 v/v) | |
B5 | NA | 1000.0 μl of water (100 v/v) | |
B6 | NA | 1000.0 μl of water (100 v/v) | |
B7 |
| pH 6.7 | 35.0 μl of 1,4-butanediol BCC (100 v/v) 40.0 μl of dimethyl sulfoxide BCC (100 v/v) 200.0 μl of lithium sulfate BCC (1 M) 325.0 μl of polyethylene glycol 400 BCC (80 v/v) 16.9 μl of sodium MES BCC pH 5.2 (1 M) 83.1 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
B8 |
| pH 6 | 325.0 μl of polyethylene glycol 400 BCC (80 v/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 88.2 μl of sodium malonate BCC (3.4 M) 5.0 μl of strontium chloride BCC (1 M) 481.8 μl of water (100 v/v) |
B9 |
| pH 8 | 300.0 μl of ammonium sulfate BCC (1 M) 325.0 μl of polyethylene glycol 400 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 275.0 μl of water (100 v/v) |
B10 |
| pH 5 | 40.0 μl of 2,5-hexanediol BCC (50 v/v) 331.2 μl of polyethylene glycol 400 BCC (80 v/v) 25.0 μl of sodium thiocyanate BCC (2 M) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 503.8 μl of water (100 v/v) |
B11 |
| pH 7.8 | 50.0 μl of 1,4-butanediol BCC (100 v/v) 337.5 μl of polyethylene glycol 400 BCC (80 v/v) 55.0 μl of sodium formate BCC (4 M) 71.1 μl of tris chloride BCC pH 7 (1 M) 28.9 μl of tris chloride BCC pH 9 (1 M) 457.5 μl of water (100 v/v) |
B12 |
| pH 6.9 | 150.0 μl of ammonium fluoride BCC (1 M) 50.0 μl of jeffamine M-600 BCC pH 7 (50 v/v) 337.5 μl of polyethylene glycol 400 BCC (80 v/v) 86.6 μl of sodium HEPES BCC pH 6.5 (1 M) 13.4 μl of sodium HEPES BCC pH 8.5 (1 M) 362.5 μl of water (100 v/v) |
C1 | NA | 1000.0 μl of water (100 v/v) | |
C2 | NA | 1000.0 μl of water (100 v/v) | |
C3 | NA | 1000.0 μl of water (100 v/v) | |
C4 | NA | 1000.0 μl of water (100 v/v) | |
C5 | NA | 1000.0 μl of water (100 v/v) | |
C6 | NA | 1000.0 μl of water (100 v/v) | |
C7 |
| pH 4 | 200.0 μl of lithium sulfate BCC (1 M) 362.5 μl of polyethylene glycol 400 BCC (80 v/v) 21.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 29.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 387.5 μl of water (100 v/v) |
C8 |
| pH 7.8 | 50.0 μl of 1,4-butanediol BCC (100 v/v) 368.8 μl of polyethylene glycol 400 BCC (80 v/v) 87.5 μl of sodium formate BCC (4 M) 71.1 μl of tris chloride BCC pH 7 (1 M) 28.9 μl of tris chloride BCC pH 9 (1 M) 393.8 μl of water (100 v/v) |
C9 |
| pH 8 | 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 58.8 μl of sodium malonate BCC (3.4 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 466.2 μl of water (100 v/v) |
C10 |
| pH 6.5 | 185.0 μl of lithium sulfate BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 96.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 4.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 340.0 μl of water (100 v/v) |
C11 |
| pH 5 | 200.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 68.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 32.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 325.0 μl of water (100 v/v) |
C12 |
| pH 5.5 | 200.0 μl of lithium sulfate BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 325.0 μl of water (100 v/v) |
D1 | NA | 1000.0 μl of water (100 v/v) | |
D2 | NA | 1000.0 μl of water (100 v/v) | |
D3 | NA | 1000.0 μl of water (100 v/v) | |
D4 | NA | 1000.0 μl of water (100 v/v) | |
D5 | NA | 1000.0 μl of water (100 v/v) | |
D6 | NA | 1000.0 μl of water (100 v/v) | |
D7 |
| pH 6.3 | 100.0 μl of ammonium formate BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 40.7 μl of sodium MES BCC pH 5.2 (1 M) 59.3 μl of sodium MES BCC pH 7.2 (1 M) 425.0 μl of water (100 v/v) |
D8 |
| pH 7 | 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 50.0 μl of sodium thiocyanate BCC (2 M) 475.0 μl of water (100 v/v) |
D9 |
| pH 6.5 | 20.0 μl of calcium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 50.0 μl of sodium thiocyanate BCC (2 M) 455.0 μl of water (100 v/v) |
D10 |
| pH 8 | 20.0 μl of calcium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium thiocyanate BCC (2 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 455.0 μl of water (100 v/v) |
D11 |
| pH 5.5 | 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 100.0 μl of sodium chloride BCC (1 M) 90.0 μl of trehalose BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 335.0 μl of water (100 v/v) |
D12 |
| pH 6.5 | 100.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 475.0 μl of water (100 v/v) |
E1 | NA | 1000.0 μl of water (100 v/v) | |
E2 | NA | 1000.0 μl of water (100 v/v) | |
E3 | NA | 1000.0 μl of water (100 v/v) | |
E4 | NA | 1000.0 μl of water (100 v/v) | |
E5 | NA | 1000.0 μl of water (100 v/v) | |
E6 | NA | 1000.0 μl of water (100 v/v) | |
E7 |
| pH 4.5 | 130.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 400 BCC (80 v/v) 75.0 μl of sodium chloride BCC (1 M) 56.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 44.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 295.0 μl of water (100 v/v) |
E8 |
| pH 6.8 | 60.0 μl of 1,4-butanediol BCC (100 v/v) 86.0 μl of bis-tris propane chloride BCC pH 6 (1 M) 14.0 μl of bis-tris propane chloride BCC pH 10 (1 M) 406.2 μl of polyethylene glycol 400 BCC (80 v/v) 125.0 μl of sodium sulfate BCC (1.2 M) 308.8 μl of water (100 v/v) |
E9 |
| pH 6.2 | 50.0 μl of ethylene glycol BCC (100 v/v) 406.2 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of potassium sodium tartrate BCC (2 M) 47.6 μl of sodium MES BCC pH 5.2 (1 M) 52.4 μl of sodium MES BCC pH 7.2 (1 M) 393.8 μl of water (100 v/v) |
E10 |
| pH 8.7 | 120.0 μl of ammonium sulfate BCC (1 M) 425.0 μl of polyethylene glycol 400 BCC (80 v/v) 10.9 μl of tris chloride BCC pH 7 (1 M) 89.1 μl of tris chloride BCC pH 9 (1 M) 90.0 μl of trisodium citrate BCC (1 M) 265.0 μl of water (100 v/v) |
E11 |
| pH 7 | 75.0 μl of dimethyl sulfoxide BCC (100 v/v) 300.0 μl of lithium sulfate BCC (1 M) 425.0 μl of polyethylene glycol 400 BCC (80 v/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v) |
E12 |
| pH 8.2 | 50.0 μl of glycerol BCC (80 v/v) 437.5 μl of polyethylene glycol 400 BCC (80 v/v) 47.6 μl of tricine BCC pH 7.2 (1 M) 52.4 μl of tricine BCC pH 9.2 (1 M) 80.0 μl of trisodium citrate BCC (1 M) 332.5 μl of water (100 v/v) |
F1 | NA | 1000.0 μl of water (100 v/v) | |
F2 | NA | 1000.0 μl of water (100 v/v) | |
F3 | NA | 1000.0 μl of water (100 v/v) | |
F4 | NA | 1000.0 μl of water (100 v/v) | |
F5 | NA | 1000.0 μl of water (100 v/v) | |
F6 | NA | 1000.0 μl of water (100 v/v) | |
F7 |
| pH 8 | 10.5 μl of 1,7-heptanediol BCC (95 v/v) 40.0 μl of dimethyl sulfoxide BCC (100 v/v) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 349.5 μl of water (100 v/v) |
F8 |
| pH 6.5 | 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 320.0 μl of sodium chloride BCC (5 M) 130.0 μl of water (100 v/v) |
F9 |
| pH 6.5 | 109.4 μl of ADA BCC pH 5.5 (0.5 M) 90.6 μl of ADA BCC pH 7.5 (0.5 M) 160.0 μl of lithium chloride BCC (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 4.0 μl of strontium chloride BCC (1 M) 136.0 μl of water (100 v/v) |
F10 |
| pH 6.5 | 109.4 μl of ADA BCC pH 5.5 (0.5 M) 90.6 μl of ADA BCC pH 7.5 (0.5 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 400 BCC (80 v/v) 100.0 μl of water (100 v/v) |
F11 |
| pH 6.5 | 525.0 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 150.0 μl of sodium acetate BCC (1 M) 225.0 μl of water (100 v/v) |
F12 |
| pH 6 | 200.0 μl of ammonium formate BCC (1 M) 10.0 μl of copper(II) chloride BCC (1 M) 312.5 μl of polyethylene glycol dimethyl ether 500 BCC (80 v/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 377.5 μl of water (100 v/v) |
G1 | NA | 1000.0 μl of water (100 v/v) | |
G2 | NA | 1000.0 μl of water (100 v/v) | |
G3 | NA | 1000.0 μl of water (100 v/v) | |
G4 | NA | 1000.0 μl of water (100 v/v) | |
G5 | NA | 1000.0 μl of water (100 v/v) | |
G6 | NA | 1000.0 μl of water (100 v/v) | |
G7 |
| pH 7 | 21.2 μl of ADA BCC pH 5.5 (0.5 M) 78.8 μl of ADA BCC pH 7.5 (0.5 M) 550.0 μl of ammonium sulfate BCC (1 M) 329.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 20.6 μl of water (100 v/v) |
G8 |
| pH 7 | 42.5 μl of ADA BCC pH 5.5 (0.5 M) 157.5 μl of ADA BCC pH 7.5 (0.5 M) 500.0 μl of polyethylene glycol 600 BCC (50 w/v) 300.0 μl of water (100 v/v) |
G9 |
| pH 5.5 | 450.0 μl of polyethylene glycol 1500 BCC (50 w/v) 90.0 μl of sodium MES BCC pH 5.2 (1 M) 10.0 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of sodium acetate BCC (1 M) 150.0 μl of water (100 v/v) |
G10 |
| pH 4.6 | 240.0 μl of polyethylene glycol 3350 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 58.8 μl of sodium malonate BCC (3.4 M) 601.2 μl of water (100 v/v) |
G11 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
G12 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
H1 | NA | 1000.0 μl of water (100 v/v) | |
H2 | NA | 1000.0 μl of water (100 v/v) | |
H3 | NA | 1000.0 μl of water (100 v/v) | |
H4 | NA | 1000.0 μl of water (100 v/v) | |
H5 | NA | 1000.0 μl of water (100 v/v) | |
H6 | NA | 1000.0 μl of water (100 v/v) | |
H7 |
| pH 5.6 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 24.0 μl of magnesium acetate BCC (2.5 M) 20.0 μl of sodium chloride BCC (5 M) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 806.0 μl of water (100 v/v) |
H8 |
| pH 6.7 | 80.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 81.2 μl of ADA BCC pH 5.5 (0.5 M) 118.8 μl of ADA BCC pH 7.5 (0.5 M) 400.0 μl of potassium nitrate BCC (1 M) 100.0 μl of tripotassium citrate BCC (1 M) 220.0 μl of water (100 v/v) |
H9 |
| pH 5.1 | 326.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (3 M) 6.7 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (3 M) 300.0 μl of trehalose BCC (1 M) 366.7 μl of water (100 v/v) |
H10 |
| pH 4.6 | 28.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 46.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 300.0 μl of sodium chloride BCC (5 M) 625.0 μl of water (100 v/v) |
H11 |
| pH 6.5 | 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 333.3 μl of sodium acetate BCC (3 M) 566.7 μl of water (100 v/v) |
H12 |
| pH 8.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 10.0 μl of nickel(II) chloride BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 490.0 μl of water (100 v/v) |