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Supplemental Information

Screen Description

Shotgun_CSIRO

BCC Core Screens / Shotgun_CSIRO
Created 2025-07-04.
This is the new Shotgun as of May 2021 - the updated 96 most successful commercial conditions. Also available and known at Molecular Dimensions as SG2.

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Shotgun_CSIRO

  • Z0002782 Lot 5 (1500.0 µl)
  • Z0002783 Lot 6 (1500.0 µl)
  • Z0002738 Lot 2 (0.0 µl)
  • Z0002756 Lot 3 (0.0 µl)
  • Z0002757 Lot 4 (0.0 µl)

Screen Structure

Shotgun_CSIRO

Well Count 96
Number of distinct ingredients 52
Internal diversity (score mean dist) 0.929

Screen Composition

2025-08-15T13:32:49.398099 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Shotgun_CSIRO
Buffer 82
Complex ingredient 1
Cryoprotectant 1
Organic (non-volatile) 7
Organic (volatile) 3
Polymer 72
Salt 82

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Shotgun_CSIRO Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
600.0 μl of polyethylene glycol 4000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
A2
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-propanol BCC (80 v/v)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
375.0 μl of water (100 v/v)
A3
  • polyethylene glycol 4000 BCC 30 w/v -
  • sodium acetate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of polyethylene glycol 4000 BCC (50 w/v)
200.0 μl of sodium acetate BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of ammonium sulfate BCC (3.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
328.6 μl of water (100 v/v)
A5
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
25.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
303.6 μl of water (100 v/v)
A7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of lithium sulfate BCC (2.5 M)
600.0 μl of polyethylene glycol 4000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
220.0 μl of water (100 v/v)
A8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 1.4 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
875.0 μl of trisodium citrate BCC (1.6 M)
25.0 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
57.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
342.9 μl of water (100 v/v)
A10
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of water (100 v/v)
A11
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
420.0 μl of water (100 v/v)
A12
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
B1
  • magnesium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6.5
80.0 μl of magnesium acetate BCC (2.5 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
420.0 μl of water (100 v/v)
B2
  • polyethylene glycol 1500 BCC 25 w/v -
NA
500.0 μl of polyethylene glycol 1500 BCC (50 w/v)
500.0 μl of water (100 v/v)
B3
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
57.1 μl of ammonium sulfate BCC (3.5 M)
600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
242.9 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
571.4 μl of ammonium sulfate BCC (3.5 M)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
328.6 μl of water (100 v/v)
B7
  • ammonium sulfate BCC 2 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
428.6 μl of water (100 v/v)
B8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
80.0 μl of lithium sulfate BCC (2.5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
320.0 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
457.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of dioxane BCC (100 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
342.9 μl of water (100 v/v)
B10
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 10000 BCC 20 w/v -
pH 7.5
666.7 μl of polyethylene glycol 10000 BCC (30 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B11
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
328.6 μl of water (100 v/v)
B12
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
57.1 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of polyethylene glycol 4000 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
342.9 μl of water (100 v/v)
C1
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
40.0 μl of ammonium acetate BCC (5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
360.0 μl of water (100 v/v)
C2
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
40.0 μl of ammonium acetate BCC (5 M)
600.0 μl of polyethylene glycol 4000 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
260.0 μl of water (100 v/v)
C3
  • polyethylene glycol 3350 BCC 20 w/v -
  • trisodium citrate BCC 0.2 M -
NA
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
125.0 μl of trisodium citrate BCC (1.6 M)
475.0 μl of water (100 v/v)
C4
  • sodium formate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium formate BCC (4 M)
400.0 μl of water (100 v/v)
C5
  • sodium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of sodium acetate BCC (1 M)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
100.0 μl of water (100 v/v)
C6
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of calcium chloride BCC (1 M)
350.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
C7
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 7.5
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
80.0 μl of lithium sulfate BCC (2.5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
C9
  • ammonium sulfate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
57.1 μl of ammonium sulfate BCC (3.5 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
242.9 μl of water (100 v/v)
C10
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
C11
  • calcium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 18 w/v -
pH 6.5
200.0 μl of calcium acetate BCC (1 M)
360.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
340.0 μl of water (100 v/v)
C12
  • lithium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of lithium sulfate BCC (2.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
57.1 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
500.0 μl of polyethylene glycol 6000 BCC (20 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
D3
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
200.0 μl of water (100 v/v)
D4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of magnesium chloride BCC (1 M)
375.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
325.0 μl of water (100 v/v)
D5
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
D6
  • polyethylene glycol 3350 BCC 20 w/v -
  • triammonium citrate-citric acid BCC 0.2 M pH 7
pH 7
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
196.0 μl of triammonium citrate-citric acid BCC pH 8.1 (1 M)
4.0 μl of triammonium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
D7
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
D8
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
640.0 μl of magnesium sulfate BCC (2.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
260.0 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 2 M -
  • potassium sodium tartrate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of potassium sodium tartrate BCC (2 M)
82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
228.6 μl of water (100 v/v)
D10
  • ammonium acetate BCC 0.1 M -
  • polyethylene glycol 10000 BCC 17 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
20.0 μl of ammonium acetate BCC (5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
566.7 μl of polyethylene glycol 10000 BCC (30 w/v)
313.3 μl of water (100 v/v)
D11
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
500.0 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 2.4 M pH 7
pH 7
705.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
294.1 μl of water (100 v/v)
E1
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
E2
  • ethylene glycol BCC 8 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7.5
80.0 μl of ethylene glycol BCC (100 v/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
620.0 μl of water (100 v/v)
E3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
40.0 μl of ammonium acetate BCC (5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
E4
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium sodium tartrate BCC 0.2 M -
NA
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of potassium sodium tartrate BCC (2 M)
500.0 μl of water (100 v/v)
E5
  • 2-propanol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
250.0 μl of 2-propanol BCC (80 v/v)
400.0 μl of polyethylene glycol 4000 BCC (50 w/v)
82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250.0 μl of water (100 v/v)
E6
  • sodium cacodylate BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
275.0 μl of water (100 v/v)
E7
  • sodium formate BCC 4 M -
NA
1000.0 μl of sodium formate BCC (4 M)
E8
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of magnesium formate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
E9
  • polyethylene glycol monomethyl ether 2000 BCC 28 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
560.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
340.0 μl of water (100 v/v)
E10
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium formate BCC 0.2 M -
NA
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
50.0 μl of sodium formate BCC (4 M)
550.0 μl of water (100 v/v)
E11
  • polyethylene glycol 4000 BCC 8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
160.0 μl of polyethylene glycol 4000 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
740.0 μl of water (100 v/v)
E12
  • polyethylene glycol 3000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of polyethylene glycol 3000 BCC (50 w/v)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1.6 M -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457.1 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
20.0 μl of sodium chloride BCC (5 M)
422.9 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
57.1 μl of ammonium sulfate BCC (3.5 M)
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
242.9 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 0.5 M -
  • lithium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
142.9 μl of ammonium sulfate BCC (3.5 M)
400.0 μl of lithium sulfate BCC (2.5 M)
82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
357.1 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
40.0 μl of sodium chloride BCC (5 M)
288.6 μl of water (100 v/v)
F5
  • polyethylene glycol 3350 BCC 25 w/v -
NA
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
500.0 μl of water (100 v/v)
F6
  • ammonium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of ammonium chloride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
F7
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • calcium chloride BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of calcium chloride BCC (1 M)
37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
580.0 μl of water (100 v/v)
F8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
50.0 μl of calcium chloride BCC (1 M)
352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497.1 μl of water (100 v/v)
F9
  • calcium acetate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6
200.0 μl of calcium acetate BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
F10
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
57.1 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
342.9 μl of water (100 v/v)
F11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of lithium sulfate BCC (2.5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
320.0 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium thiocyanate BCC 0.2 M -
NA
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of potassium thiocyanate BCC (2 M)
500.0 μl of water (100 v/v)
G1
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
40.0 μl of sodium chloride BCC (5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
360.0 μl of water (100 v/v)
G2
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
100.0 μl of diammonium hydrogen citrate BCC (2 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
500.0 μl of water (100 v/v)
G3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40.0 μl of ammonium acetate BCC (5 M)
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
360.0 μl of water (100 v/v)
G4
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 7
pH 7
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
58.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
541.2 μl of water (100 v/v)
G5
  • tacsimate BCC 60 v/v pH 7
pH 7
600.0 μl of tacsimate BCC pH 7 (100 v/v)
400.0 μl of water (100 v/v)
G6
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 30 w/v -
pH 7
600.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G7
  • sodium DL-malate BCC 2.1 M pH 7
pH 7
840.0 μl of sodium DL-malate BCC pH 7 (2.5 M)
160.0 μl of water (100 v/v)
G8
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium thiocyanate BCC 0.2 M -
NA
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of sodium thiocyanate BCC (2 M)
500.0 μl of water (100 v/v)
G9
  • sodium chloride BCC 4.3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
860.0 μl of sodium chloride BCC (5 M)
40.0 μl of water (100 v/v)
G10
  • sodium formate BCC 3.5 M -
NA
875.0 μl of sodium formate BCC (4 M)
125.0 μl of water (100 v/v)
G11
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50.0 μl of potassium thiocyanate BCC (2 M)
350.0 μl of water (100 v/v)
G12
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of magnesium chloride BCC (1 M)
352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
497.1 μl of water (100 v/v)
H1
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
200.0 μl of calcium chloride BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H2
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
475.0 μl of water (100 v/v)
H3
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • proline BCC 0.2 M -
pH 7.5
200.0 μl of polyethylene glycol 3350 BCC (50 w/v)
200.0 μl of proline BCC (1 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 12 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of ammonium sulfate BCC (3.5 M)
150.0 μl of glycerol BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
321.4 μl of water (100 v/v)
H5
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • zinc sulfate BCC 0.01 M -
pH 6.5
294.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
100.0 μl of zinc sulfate BCC (0.1 M)
505.9 μl of water (100 v/v)
H6
  • phosphate-citrate BCC 0.1 M pH 4.2
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 20 w/v -
pH 4.2
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
H7
  • polyethylene glycol 3350 BCC 20 w/v -
  • ammonium formate BCC 0.2 M -
NA
200.0 μl of ammonium formate BCC (1 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
400.0 μl of water (100 v/v)
H8
  • polyethylene glycol 3350 BCC 25 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
500.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
H10
  • calcium acetate BCC 0.16 M -
  • glycerol BCC 20 v/v -
  • sodium cacodylate BCC 0.08 M pH 6.5
  • polyethylene glycol 8000 BCC 14.4 w/v -
pH 6.5
160.0 μl of calcium acetate BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
288.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
52.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
222.0 μl of water (100 v/v)
H11
  • magnesium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
80.0 μl of magnesium acetate BCC (2.5 M)
400.0 μl of polyethylene glycol 3350 BCC (50 w/v)
520.0 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 8 w/v -
pH 8.5
160.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
740.0 μl of water (100 v/v)