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Supplemental Information

Screen Description

Shotgun_CSIRO

BCC Core Screens / Shotgun_CSIRO
Created 2025-03-07.
This is the new Shotgun as of May 2021 - the updated 96 most successful commercial conditions. Also available and known at Molecular Dimensions as SG2.

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Barcodes

Shotgun_CSIRO

  • Z0002738
  • Z0002756
  • Z0002757
  • Z0002782
  • Z0002783

Screen Structure

Shotgun_CSIRO

Well Count 96
Number of distinct ingredients 52
Internal diversity (score mean dist) 0.929

Screen Composition

2025-06-14T13:41:22.103676 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Shotgun_CSIRO
Buffer 82
Complex ingredient 1
Cryoprotectant 1
Organic (non-volatile) 7
Organic (volatile) 3
Polymer 72
Salt 82

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Shotgun_CSIRO Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of magnesium chloride BCC (1 M)
600 μl of polyethylene glycol 4000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
100 μl of water (100 v/v)
A2
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125 μl of 2-propanol BCC (80 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
375 μl of water (100 v/v)
A3
  • polyethylene glycol 4000 BCC 30 w/v -
  • sodium acetate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of sodium acetate BCC (1 M)
600 μl of polyethylene glycol 4000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
100 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
328 μl of water (100 v/v)
A5
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
400 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
25 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
303 μl of water (100 v/v)
A7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
600 μl of polyethylene glycol 4000 BCC (50 w/v)
80 μl of lithium sulfate BCC (2.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
220 μl of water (100 v/v)
A8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 1.4 M -
pH 7.5
875 μl of trisodium citrate BCC (1.6 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
25 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
57 μl of ammonium sulfate BCC (3.5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
342 μl of water (100 v/v)
A10
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
200 μl of water (100 v/v)
A11
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
420 μl of water (100 v/v)
A12
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
40 μl of sodium chloride BCC (5 M)
360 μl of water (100 v/v)
B1
  • magnesium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
80 μl of magnesium acetate BCC (2.5 M)
420 μl of water (100 v/v)
B2
  • polyethylene glycol 1500 BCC 25 w/v -
NA
500 μl of polyethylene glycol 1500 BCC (50 w/v)
500 μl of water (100 v/v)
B3
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
600 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
57 μl of ammonium sulfate BCC (3.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
242 μl of water (100 v/v)
B5
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
200 μl of magnesium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
571 μl of ammonium sulfate BCC (3.5 M)
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
328 μl of water (100 v/v)
B7
  • ammonium sulfate BCC 2 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
428 μl of water (100 v/v)
B8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
80 μl of lithium sulfate BCC (2.5 M)
320 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
100 μl of dioxane BCC (100 v/v)
72 μl of sodium MES BCC pH 7.2 (1 M)
342 μl of water (100 v/v)
B10
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 10000 BCC 20 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
666 μl of polyethylene glycol 10000 BCC (30 w/v)
233 μl of water (100 v/v)
B11
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
328 μl of water (100 v/v)
B12
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 25 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
57 μl of ammonium sulfate BCC (3.5 M)
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500 μl of polyethylene glycol 4000 BCC (50 w/v)
342 μl of water (100 v/v)
C1
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
40 μl of ammonium acetate BCC (5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
360 μl of water (100 v/v)
C2
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 4000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
40 μl of ammonium acetate BCC (5 M)
600 μl of polyethylene glycol 4000 BCC (50 w/v)
260 μl of water (100 v/v)
C3
  • polyethylene glycol 3350 BCC 20 w/v -
  • trisodium citrate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
125 μl of trisodium citrate BCC (1.6 M)
475 μl of water (100 v/v)
C4
  • sodium formate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500 μl of sodium formate BCC (4 M)
400 μl of water (100 v/v)
C5
  • sodium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
200 μl of sodium acetate BCC (1 M)
600 μl of polyethylene glycol 8000 BCC (50 w/v)
100 μl of water (100 v/v)
C6
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 28 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
350 μl of polyethylene glycol 400 BCC (80 v/v)
200 μl of calcium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
350 μl of water (100 v/v)
C7
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
500 μl of water (100 v/v)
C8
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
80 μl of lithium sulfate BCC (2.5 M)
320 μl of water (100 v/v)
C9
  • ammonium sulfate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
600 μl of polyethylene glycol 8000 BCC (50 w/v)
242 μl of water (100 v/v)
C10
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of water (100 v/v)
C11
  • calcium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 18 w/v -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
200 μl of calcium acetate BCC (1 M)
360 μl of polyethylene glycol 8000 BCC (50 w/v)
340 μl of water (100 v/v)
C12
  • lithium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
600 μl of lithium sulfate BCC (2.5 M)
300 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
342 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 6000 BCC (20 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
350 μl of water (100 v/v)
D3
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
200 μl of water (100 v/v)
D4
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 400 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
200 μl of magnesium chloride BCC (1 M)
375 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
325 μl of water (100 v/v)
D5
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of magnesium chloride BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
300 μl of water (100 v/v)
D6
  • polyethylene glycol 3350 BCC 20 w/v -
  • triammonium citrate-citric acid BCC 0.2 M pH 7
pH 7
400 μl of polyethylene glycol 3350 BCC (50 w/v)
4 μl of triammonium citrate-citric acid BCC pH 2.3 (1 M)
196 μl of triammonium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of water (100 v/v)
D7
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
328 μl of water (100 v/v)
D8
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
27 μl of sodium MES BCC pH 5.2 (1 M)
640 μl of magnesium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
260 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 2 M -
  • potassium sodium tartrate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
100 μl of potassium sodium tartrate BCC (2 M)
571 μl of ammonium sulfate BCC (3.5 M)
82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
228 μl of water (100 v/v)
D10
  • ammonium acetate BCC 0.1 M -
  • polyethylene glycol 10000 BCC 17 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
20 μl of ammonium acetate BCC (5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
566 μl of polyethylene glycol 10000 BCC (30 w/v)
313 μl of water (100 v/v)
D11
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 2.4 M pH 7
pH 7
705 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
294 μl of water (100 v/v)
E1
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
40 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
360 μl of water (100 v/v)
E2
  • ethylene glycol BCC 8 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
80 μl of ethylene glycol BCC (100 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
620 μl of water (100 v/v)
E3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
40 μl of ammonium acetate BCC (5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
360 μl of water (100 v/v)
E4
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium sodium tartrate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of potassium sodium tartrate BCC (2 M)
500 μl of water (100 v/v)
E5
  • 2-propanol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
250 μl of 2-propanol BCC (80 v/v)
82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of polyethylene glycol 4000 BCC (50 w/v)
18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
250 μl of water (100 v/v)
E6
  • sodium cacodylate BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
625 μl of trisodium citrate BCC (1.6 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
275 μl of water (100 v/v)
E7
  • sodium formate BCC 4 M -
NA
1000 μl of sodium formate BCC (4 M)
E8
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of magnesium formate BCC (1 M)
400 μl of water (100 v/v)
E9
  • polyethylene glycol monomethyl ether 2000 BCC 28 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
560 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
340 μl of water (100 v/v)
E10
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium formate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium formate BCC (4 M)
550 μl of water (100 v/v)
E11
  • polyethylene glycol 4000 BCC 8 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
160 μl of polyethylene glycol 4000 BCC (50 w/v)
740 μl of water (100 v/v)
E12
  • polyethylene glycol 3000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of polyethylene glycol 3000 BCC (50 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1.6 M -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
20 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
422 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
57 μl of ammonium sulfate BCC (3.5 M)
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
242 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 0.5 M -
  • lithium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.6
pH 5.6
142 μl of ammonium sulfate BCC (3.5 M)
82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of lithium sulfate BCC (2.5 M)
357 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
40 μl of sodium chloride BCC (5 M)
288 μl of water (100 v/v)
F5
  • polyethylene glycol 3350 BCC 25 w/v -
NA
500 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
F6
  • ammonium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of ammonium chloride BCC (1 M)
400 μl of water (100 v/v)
F7
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • calcium chloride BCC 0.02 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.6
pH 4.6
37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
20 μl of calcium chloride BCC (1 M)
300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
580 μl of water (100 v/v)
F8
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
50 μl of calcium chloride BCC (1 M)
497 μl of water (100 v/v)
F9
  • calcium acetate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6
61 μl of sodium MES BCC pH 5.2 (1 M)
200 μl of calcium acetate BCC (1 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
300 μl of water (100 v/v)
F10
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
342 μl of water (100 v/v)
F11
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
80 μl of lithium sulfate BCC (2.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
320 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium thiocyanate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of potassium thiocyanate BCC (2 M)
500 μl of water (100 v/v)
G1
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
40 μl of sodium chloride BCC (5 M)
21 μl of tris chloride BCC pH 7 (1 M)
360 μl of water (100 v/v)
G2
  • diammonium hydrogen citrate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of diammonium hydrogen citrate BCC (2 M)
500 μl of water (100 v/v)
G3
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
40 μl of ammonium acetate BCC (5 M)
21 μl of tris chloride BCC pH 7 (1 M)
360 μl of water (100 v/v)
G4
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 7
pH 7
400 μl of polyethylene glycol 3350 BCC (50 w/v)
58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
541 μl of water (100 v/v)
G5
  • tacsimate BCC 60 v/v pH 7
pH 7
600 μl of tacsimate BCC pH 7 (100 v/v)
400 μl of water (100 v/v)
G6
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 30 w/v -
pH 7
600 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
G7
  • sodium DL-malate BCC 2.1 M pH 7
pH 7
840 μl of sodium DL-malate BCC pH 7 (2.5 M)
160 μl of water (100 v/v)
G8
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium thiocyanate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of sodium thiocyanate BCC (2 M)
500 μl of water (100 v/v)
G9
  • sodium chloride BCC 4.3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
860 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
40 μl of water (100 v/v)
G10
  • sodium formate BCC 3.5 M -
NA
875 μl of sodium formate BCC (4 M)
125 μl of water (100 v/v)
G11
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50 μl of potassium thiocyanate BCC (2 M)
350 μl of water (100 v/v)
G12
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of magnesium chloride BCC (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
497 μl of water (100 v/v)
H1
  • calcium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of calcium chloride BCC (1 M)
400 μl of water (100 v/v)
H2
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 9.5
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
475 μl of water (100 v/v)
H3
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • proline BCC 0.2 M -
pH 7.5
200 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of proline BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 12 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
150 μl of glycerol BCC (80 v/v)
21 μl of tris chloride BCC pH 7 (1 M)
321 μl of water (100 v/v)
H5
  • polyethylene glycol monomethyl ether 550 BCC 25 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • zinc sulfate BCC 0.01 M -
pH 6.5
294 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
100 μl of zinc sulfate BCC (0.1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
505 μl of water (100 v/v)
H6
  • phosphate-citrate BCC 0.1 M pH 4.2
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 20 w/v -
pH 4.2
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
40 μl of sodium chloride BCC (5 M)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
360 μl of water (100 v/v)
H7
  • polyethylene glycol 3350 BCC 20 w/v -
  • ammonium formate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of ammonium formate BCC (1 M)
400 μl of water (100 v/v)
H8
  • polyethylene glycol 3350 BCC 25 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of water (100 v/v)
H10
  • calcium acetate BCC 0.16 M -
  • glycerol BCC 20 v/v -
  • sodium cacodylate BCC 0.08 M pH 6.5
  • polyethylene glycol 8000 BCC 14.4 w/v -
pH 6.5
27 μl of sodium cacodylate BCC pH 5.3 (1 M)
52 μl of sodium cacodylate BCC pH 7.3 (1 M)
160 μl of calcium acetate BCC (1 M)
250 μl of glycerol BCC (80 v/v)
288 μl of polyethylene glycol 8000 BCC (50 w/v)
222 μl of water (100 v/v)
H11
  • magnesium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 20 w/v -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
80 μl of magnesium acetate BCC (2.5 M)
520 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 8 w/v -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
160 μl of polyethylene glycol 8000 BCC (50 w/v)
740 μl of water (100 v/v)