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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Shotgun_CSIRO
BCC Core Screens / Shotgun_CSIRO
Created 2025-07-04.
This is the new Shotgun as of May 2021 - the updated 96 most successful commercial conditions. Also available and known at Molecular Dimensions as SG2.
Export
Shotgun_CSIRO
Barcodes
Shotgun_CSIRO
Screen Structure
Shotgun_CSIRO
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.929 |
Screen Composition
ingredient type | Shotgun_CSIRO |
---|---|
Buffer | 82 |
Complex ingredient | 1 |
Cryoprotectant | 1 |
Organic (non-volatile) | 7 |
Organic (volatile) | 3 |
Polymer | 72 |
Salt | 82 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
A2 |
| pH 7.5 | 125.0 μl of 2-propanol BCC (80 v/v) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 375.0 μl of water (100 v/v) |
A3 |
| pH 8.5 | 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
A4 |
| pH 8.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 328.6 μl of water (100 v/v) |
A5 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
A6 |
| pH 7.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 25.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 303.6 μl of water (100 v/v) |
A7 |
| pH 8.5 | 80.0 μl of lithium sulfate BCC (2.5 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 220.0 μl of water (100 v/v) |
A8 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 875.0 μl of trisodium citrate BCC (1.6 M) 25.0 μl of water (100 v/v) |
A9 |
| pH 5.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 342.9 μl of water (100 v/v) |
A10 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of water (100 v/v) |
A11 |
| pH 6.5 | 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 420.0 μl of water (100 v/v) |
A12 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
B1 |
| pH 6.5 | 80.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 420.0 μl of water (100 v/v) |
B2 |
| NA | 500.0 μl of polyethylene glycol 1500 BCC (50 w/v) 500.0 μl of water (100 v/v) |
B3 |
| pH 7.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
B4 |
| pH 6.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 242.9 μl of water (100 v/v) |
B5 |
| pH 7.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
B6 |
| pH 4.6 | 571.4 μl of ammonium sulfate BCC (3.5 M) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 328.6 μl of water (100 v/v) |
B7 |
| NA | 571.4 μl of ammonium sulfate BCC (3.5 M) 428.6 μl of water (100 v/v) |
B8 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 80.0 μl of lithium sulfate BCC (2.5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 320.0 μl of water (100 v/v) |
B9 |
| pH 6.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of dioxane BCC (100 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 342.9 μl of water (100 v/v) |
B10 |
| pH 7.5 | 666.7 μl of polyethylene glycol 10000 BCC (30 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
B11 |
| pH 5.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 328.6 μl of water (100 v/v) |
B12 |
| pH 4.6 | 57.1 μl of ammonium sulfate BCC (3.5 M) 500.0 μl of polyethylene glycol 4000 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 342.9 μl of water (100 v/v) |
C1 |
| pH 5.5 | 40.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 360.0 μl of water (100 v/v) |
C2 |
| pH 4.6 | 40.0 μl of ammonium acetate BCC (5 M) 600.0 μl of polyethylene glycol 4000 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 260.0 μl of water (100 v/v) |
C3 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 125.0 μl of trisodium citrate BCC (1.6 M) 475.0 μl of water (100 v/v) |
C4 |
| pH 4.6 | 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium formate BCC (4 M) 400.0 μl of water (100 v/v) |
C5 |
| pH 6.5 | 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 100.0 μl of water (100 v/v) |
C6 |
| pH 7.5 | 200.0 μl of calcium chloride BCC (1 M) 350.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
C7 |
| pH 7.5 | 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
C8 |
| pH 7.5 | 80.0 μl of lithium sulfate BCC (2.5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
C9 |
| pH 6.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 242.9 μl of water (100 v/v) |
C10 |
| pH 8.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 400.0 μl of water (100 v/v) |
C11 |
| pH 6.5 | 200.0 μl of calcium acetate BCC (1 M) 360.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 340.0 μl of water (100 v/v) |
C12 |
| pH 7.5 | 600.0 μl of lithium sulfate BCC (2.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
D1 |
| pH 7.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 342.9 μl of water (100 v/v) |
D2 |
| pH 7.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500.0 μl of polyethylene glycol 6000 BCC (20 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
D3 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 200.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 200.0 μl of magnesium chloride BCC (1 M) 375.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 325.0 μl of water (100 v/v) |
D5 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
D6 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 196.0 μl of triammonium citrate-citric acid BCC pH 8.1 (1 M) 4.0 μl of triammonium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
D7 |
| pH 7.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v) |
D8 |
| pH 6.5 | 640.0 μl of magnesium sulfate BCC (2.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 260.0 μl of water (100 v/v) |
D9 |
| pH 5.6 | 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of potassium sodium tartrate BCC (2 M) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 228.6 μl of water (100 v/v) |
D10 |
| pH 5.5 | 20.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 566.7 μl of polyethylene glycol 10000 BCC (30 w/v) 313.3 μl of water (100 v/v) |
D11 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 500.0 μl of water (100 v/v) |
D12 |
| pH 7 | 705.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 294.1 μl of water (100 v/v) |
E1 |
| pH 7.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 80.0 μl of ethylene glycol BCC (100 v/v) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 620.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 40.0 μl of ammonium acetate BCC (5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 360.0 μl of water (100 v/v) |
E4 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium sodium tartrate BCC (2 M) 500.0 μl of water (100 v/v) |
E5 |
| pH 5.6 | 250.0 μl of 2-propanol BCC (80 v/v) 400.0 μl of polyethylene glycol 4000 BCC (50 w/v) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 275.0 μl of water (100 v/v) |
E7 |
| NA | 1000.0 μl of sodium formate BCC (4 M) |
E8 |
| NA | 200.0 μl of magnesium formate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
E9 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 560.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 340.0 μl of water (100 v/v) |
E10 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 50.0 μl of sodium formate BCC (4 M) 550.0 μl of water (100 v/v) |
E11 |
| pH 4.6 | 160.0 μl of polyethylene glycol 4000 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 740.0 μl of water (100 v/v) |
E12 |
| pH 5.5 | 400.0 μl of polyethylene glycol 3000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
F1 |
| pH 7.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 20.0 μl of sodium chloride BCC (5 M) 422.9 μl of water (100 v/v) |
F2 |
| pH 4.6 | 57.1 μl of ammonium sulfate BCC (3.5 M) 600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 242.9 μl of water (100 v/v) |
F3 |
| pH 5.6 | 142.9 μl of ammonium sulfate BCC (3.5 M) 400.0 μl of lithium sulfate BCC (2.5 M) 82.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 357.1 μl of water (100 v/v) |
F4 |
| pH 6.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 40.0 μl of sodium chloride BCC (5 M) 288.6 μl of water (100 v/v) |
F5 |
| NA | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
F6 |
| NA | 200.0 μl of ammonium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
F7 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20.0 μl of calcium chloride BCC (1 M) 37.9 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62.1 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 580.0 μl of water (100 v/v) |
F8 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 50.0 μl of calcium chloride BCC (1 M) 352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 497.1 μl of water (100 v/v) |
F9 |
| pH 6 | 200.0 μl of calcium acetate BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
F10 |
| pH 8.5 | 57.1 μl of ammonium sulfate BCC (3.5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 342.9 μl of water (100 v/v) |
F11 |
| pH 8.5 | 80.0 μl of lithium sulfate BCC (2.5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 320.0 μl of water (100 v/v) |
F12 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of potassium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
G1 |
| pH 8.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 40.0 μl of sodium chloride BCC (5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 360.0 μl of water (100 v/v) |
G2 |
| NA | 100.0 μl of diammonium hydrogen citrate BCC (2 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 500.0 μl of water (100 v/v) |
G3 |
| pH 8.5 | 40.0 μl of ammonium acetate BCC (5 M) 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 360.0 μl of water (100 v/v) |
G4 |
| pH 7 | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 58.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 541.2 μl of water (100 v/v) |
G5 |
| pH 7 | 600.0 μl of tacsimate BCC pH 7 (100 v/v) 400.0 μl of water (100 v/v) |
G6 |
| pH 7 | 600.0 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G7 |
| pH 7 | 840.0 μl of sodium DL-malate BCC pH 7 (2.5 M) 160.0 μl of water (100 v/v) |
G8 |
| NA | 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 100.0 μl of sodium thiocyanate BCC (2 M) 500.0 μl of water (100 v/v) |
G9 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 860.0 μl of sodium chloride BCC (5 M) 40.0 μl of water (100 v/v) |
G10 |
| NA | 875.0 μl of sodium formate BCC (4 M) 125.0 μl of water (100 v/v) |
G11 |
| NA | 600.0 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 50.0 μl of potassium thiocyanate BCC (2 M) 350.0 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50.0 μl of magnesium chloride BCC (1 M) 352.9 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 497.1 μl of water (100 v/v) |
H1 |
| NA | 200.0 μl of calcium chloride BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
H2 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 475.0 μl of water (100 v/v) |
H3 |
| pH 7.5 | 200.0 μl of polyethylene glycol 3350 BCC (50 w/v) 200.0 μl of proline BCC (1 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
H4 |
| pH 8.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 150.0 μl of glycerol BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 321.4 μl of water (100 v/v) |
H5 |
| pH 6.5 | 294.1 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 100.0 μl of zinc sulfate BCC (0.1 M) 505.9 μl of water (100 v/v) |
H6 |
| pH 4.2 | 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
H7 |
| NA | 200.0 μl of ammonium formate BCC (1 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 400.0 μl of water (100 v/v) |
H8 |
| pH 3.5 | 500.0 μl of polyethylene glycol 3350 BCC (50 w/v) 30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v) |
H9 |
| pH 7.5 | 700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
H10 |
| pH 6.5 | 160.0 μl of calcium acetate BCC (1 M) 250.0 μl of glycerol BCC (80 v/v) 288.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 52.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 222.0 μl of water (100 v/v) |
H11 |
| NA | 80.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 3350 BCC (50 w/v) 520.0 μl of water (100 v/v) |
H12 |
| pH 8.5 | 160.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 740.0 μl of water (100 v/v) |