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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Shotgun_CSIRO
BCC Core Screens / Shotgun_CSIRO
Created 2025-03-07.
This is the new Shotgun as of May 2021 - the updated 96 most successful commercial conditions. Also available and known at Molecular Dimensions as SG2.
Export
Shotgun_CSIRO
Barcodes
Shotgun_CSIRO
- Z0002738
- Z0002756
- Z0002757
- Z0002782
- Z0002783
Screen Structure
Shotgun_CSIRO
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.929 |
Screen Composition
ingredient type | Shotgun_CSIRO |
---|---|
Buffer | 82 |
Complex ingredient | 1 |
Cryoprotectant | 1 |
Organic (non-volatile) | 7 |
Organic (volatile) | 3 |
Polymer | 72 |
Salt | 82 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 200 μl of magnesium chloride BCC (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 100 μl of water (100 v/v) |
A2 |
| pH 7.5 | 125 μl of 2-propanol BCC (80 v/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 375 μl of water (100 v/v) |
A3 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 200 μl of sodium acetate BCC (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 100 μl of water (100 v/v) |
A4 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 328 μl of water (100 v/v) |
A5 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 400 μl of water (100 v/v) |
A6 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 25 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 303 μl of water (100 v/v) |
A7 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 80 μl of lithium sulfate BCC (2.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 220 μl of water (100 v/v) |
A8 |
| pH 7.5 | 875 μl of trisodium citrate BCC (1.6 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of water (100 v/v) |
A9 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 57 μl of ammonium sulfate BCC (3.5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 342 μl of water (100 v/v) |
A10 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium chloride BCC (1 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of water (100 v/v) |
A11 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 480 μl of polyethylene glycol 20000 BCC (25 w/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 420 μl of water (100 v/v) |
A12 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 40 μl of sodium chloride BCC (5 M) 360 μl of water (100 v/v) |
B1 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 80 μl of magnesium acetate BCC (2.5 M) 420 μl of water (100 v/v) |
B2 |
| NA | 500 μl of polyethylene glycol 1500 BCC (50 w/v) 500 μl of water (100 v/v) |
B3 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
B4 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 600 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 57 μl of ammonium sulfate BCC (3.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 242 μl of water (100 v/v) |
B5 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 200 μl of magnesium chloride BCC (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of water (100 v/v) |
B6 |
| pH 4.6 | 571 μl of ammonium sulfate BCC (3.5 M) 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 328 μl of water (100 v/v) |
B7 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 428 μl of water (100 v/v) |
B8 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 80 μl of lithium sulfate BCC (2.5 M) 320 μl of water (100 v/v) |
B9 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 100 μl of dioxane BCC (100 v/v) 72 μl of sodium MES BCC pH 7.2 (1 M) 342 μl of water (100 v/v) |
B10 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 666 μl of polyethylene glycol 10000 BCC (30 w/v) 233 μl of water (100 v/v) |
B11 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 328 μl of water (100 v/v) |
B12 |
| pH 4.6 | 57 μl of ammonium sulfate BCC (3.5 M) 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500 μl of polyethylene glycol 4000 BCC (50 w/v) 342 μl of water (100 v/v) |
C1 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 40 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 360 μl of water (100 v/v) |
C2 |
| pH 4.6 | 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 40 μl of ammonium acetate BCC (5 M) 600 μl of polyethylene glycol 4000 BCC (50 w/v) 260 μl of water (100 v/v) |
C3 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 125 μl of trisodium citrate BCC (1.6 M) 475 μl of water (100 v/v) |
C4 |
| pH 4.6 | 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500 μl of sodium formate BCC (4 M) 400 μl of water (100 v/v) |
C5 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 200 μl of sodium acetate BCC (1 M) 600 μl of polyethylene glycol 8000 BCC (50 w/v) 100 μl of water (100 v/v) |
C6 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 350 μl of polyethylene glycol 400 BCC (80 v/v) 200 μl of calcium chloride BCC (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 350 μl of water (100 v/v) |
C7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 500 μl of water (100 v/v) |
C8 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 80 μl of lithium sulfate BCC (2.5 M) 320 μl of water (100 v/v) |
C9 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 600 μl of polyethylene glycol 8000 BCC (50 w/v) 242 μl of water (100 v/v) |
C10 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of water (100 v/v) |
C11 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 200 μl of calcium acetate BCC (1 M) 360 μl of polyethylene glycol 8000 BCC (50 w/v) 340 μl of water (100 v/v) |
C12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 600 μl of lithium sulfate BCC (2.5 M) 300 μl of water (100 v/v) |
D1 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 342 μl of water (100 v/v) |
D2 |
| pH 7.5 | 500 μl of polyethylene glycol 6000 BCC (20 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 350 μl of water (100 v/v) |
D3 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 200 μl of magnesium chloride BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 200 μl of water (100 v/v) |
D4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 200 μl of magnesium chloride BCC (1 M) 375 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 325 μl of water (100 v/v) |
D5 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 200 μl of magnesium chloride BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 300 μl of water (100 v/v) |
D6 |
| pH 7 | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 4 μl of triammonium citrate-citric acid BCC pH 2.3 (1 M) 196 μl of triammonium citrate-citric acid BCC pH 8.1 (1 M) 400 μl of water (100 v/v) |
D7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 328 μl of water (100 v/v) |
D8 |
| pH 6.5 | 27 μl of sodium MES BCC pH 5.2 (1 M) 640 μl of magnesium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 260 μl of water (100 v/v) |
D9 |
| pH 5.6 | 100 μl of potassium sodium tartrate BCC (2 M) 571 μl of ammonium sulfate BCC (3.5 M) 82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 228 μl of water (100 v/v) |
D10 |
| pH 5.5 | 20 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 566 μl of polyethylene glycol 10000 BCC (30 w/v) 313 μl of water (100 v/v) |
D11 |
| pH 6.5 | 400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of water (100 v/v) |
D12 |
| pH 7 | 705 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 294 μl of water (100 v/v) |
E1 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 40 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 360 μl of water (100 v/v) |
E2 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 80 μl of ethylene glycol BCC (100 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of polyethylene glycol 8000 BCC (50 w/v) 620 μl of water (100 v/v) |
E3 |
| pH 7.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 40 μl of ammonium acetate BCC (5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 360 μl of water (100 v/v) |
E4 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of potassium sodium tartrate BCC (2 M) 500 μl of water (100 v/v) |
E5 |
| pH 5.6 | 250 μl of 2-propanol BCC (80 v/v) 82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 400 μl of polyethylene glycol 4000 BCC (50 w/v) 18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 250 μl of water (100 v/v) |
E6 |
| pH 6.5 | 625 μl of trisodium citrate BCC (1.6 M) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 275 μl of water (100 v/v) |
E7 |
| NA | 1000 μl of sodium formate BCC (4 M) |
E8 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of magnesium formate BCC (1 M) 400 μl of water (100 v/v) |
E9 |
| pH 6.5 | 560 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 340 μl of water (100 v/v) |
E10 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium formate BCC (4 M) 550 μl of water (100 v/v) |
E11 |
| pH 4.6 | 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 160 μl of polyethylene glycol 4000 BCC (50 w/v) 740 μl of water (100 v/v) |
E12 |
| pH 5.5 | 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 400 μl of polyethylene glycol 3000 BCC (50 w/v) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500 μl of water (100 v/v) |
F1 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 20 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 422 μl of water (100 v/v) |
F2 |
| pH 4.6 | 57 μl of ammonium sulfate BCC (3.5 M) 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 242 μl of water (100 v/v) |
F3 |
| pH 5.6 | 142 μl of ammonium sulfate BCC (3.5 M) 82 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 18 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of lithium sulfate BCC (2.5 M) 357 μl of water (100 v/v) |
F4 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 40 μl of sodium chloride BCC (5 M) 288 μl of water (100 v/v) |
F5 |
| NA | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 500 μl of water (100 v/v) |
F6 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of ammonium chloride BCC (1 M) 400 μl of water (100 v/v) |
F7 |
| pH 4.6 | 37 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 62 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 20 μl of calcium chloride BCC (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 580 μl of water (100 v/v) |
F8 |
| pH 6.5 | 352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 50 μl of calcium chloride BCC (1 M) 497 μl of water (100 v/v) |
F9 |
| pH 6 | 61 μl of sodium MES BCC pH 5.2 (1 M) 200 μl of calcium acetate BCC (1 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 300 μl of water (100 v/v) |
F10 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 342 μl of water (100 v/v) |
F11 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 80 μl of lithium sulfate BCC (2.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 320 μl of water (100 v/v) |
F12 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of potassium thiocyanate BCC (2 M) 500 μl of water (100 v/v) |
G1 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 40 μl of sodium chloride BCC (5 M) 21 μl of tris chloride BCC pH 7 (1 M) 360 μl of water (100 v/v) |
G2 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of diammonium hydrogen citrate BCC (2 M) 500 μl of water (100 v/v) |
G3 |
| pH 8.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 40 μl of ammonium acetate BCC (5 M) 21 μl of tris chloride BCC pH 7 (1 M) 360 μl of water (100 v/v) |
G4 |
| pH 7 | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 541 μl of water (100 v/v) |
G5 |
| pH 7 | 600 μl of tacsimate BCC pH 7 (100 v/v) 400 μl of water (100 v/v) |
G6 |
| pH 7 | 600 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
G7 |
| pH 7 | 840 μl of sodium DL-malate BCC pH 7 (2.5 M) 160 μl of water (100 v/v) |
G8 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of sodium thiocyanate BCC (2 M) 500 μl of water (100 v/v) |
G9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 860 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 40 μl of water (100 v/v) |
G10 |
| NA | 875 μl of sodium formate BCC (4 M) 125 μl of water (100 v/v) |
G11 |
| NA | 600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 50 μl of potassium thiocyanate BCC (2 M) 350 μl of water (100 v/v) |
G12 |
| pH 7.5 | 352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of magnesium chloride BCC (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 497 μl of water (100 v/v) |
H1 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of calcium chloride BCC (1 M) 400 μl of water (100 v/v) |
H2 |
| pH 9.5 | 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 475 μl of water (100 v/v) |
H3 |
| pH 7.5 | 200 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of proline BCC (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of water (100 v/v) |
H4 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 150 μl of glycerol BCC (80 v/v) 21 μl of tris chloride BCC pH 7 (1 M) 321 μl of water (100 v/v) |
H5 |
| pH 6.5 | 294 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 100 μl of zinc sulfate BCC (0.1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 505 μl of water (100 v/v) |
H6 |
| pH 4.2 | 88 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 40 μl of sodium chloride BCC (5 M) 112 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 400 μl of polyethylene glycol 8000 BCC (50 w/v) 360 μl of water (100 v/v) |
H7 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of ammonium formate BCC (1 M) 400 μl of water (100 v/v) |
H8 |
| pH 3.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of water (100 v/v) |
H9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of water (100 v/v) |
H10 |
| pH 6.5 | 27 μl of sodium cacodylate BCC pH 5.3 (1 M) 52 μl of sodium cacodylate BCC pH 7.3 (1 M) 160 μl of calcium acetate BCC (1 M) 250 μl of glycerol BCC (80 v/v) 288 μl of polyethylene glycol 8000 BCC (50 w/v) 222 μl of water (100 v/v) |
H11 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 80 μl of magnesium acetate BCC (2.5 M) 520 μl of water (100 v/v) |
H12 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 160 μl of polyethylene glycol 8000 BCC (50 w/v) 740 μl of water (100 v/v) |