C6 - Screen Contents - Crystal Screen 2

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Supplemental Information

Screen Description

Crystal Screen 2

Hampton Research / Crystal Screen 2

Created 2009-11-16.

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Crystal Screen 2

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Crystal Screen 2

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Screen Structure

Crystal Screen 2

Well Count	48
Number of distinct ingredients	46
Internal diversity (score mean dist)	0.950

Screen Composition

More information about screen composition chart.

ingredient type	Crystal Screen 2
Buffer	46
Cryoprotectant	1
Detergent	1
Organic (non-volatile)	17
Organic (volatile)	6
Polymer	17
Salt	53

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Crystal Screen 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 2 M - Polyethylene glycol 6,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of Sodium chloride (5 M) 400.0 μl of water (100 v/v)
A2	Sodium chloride 0.5 M - Magnesium chloride 0.01 M - Cetyltrimethylammonium bromide 0.01 mM - 🔍	NA	0.2 μl of Cetyltrimethylammonium bromide (50 mM) 5.0 μl of Magnesium chloride (2 M) 100.0 μl of Sodium chloride (5 M) 894.8 μl of water (100 v/v)
A3	Ethylene glycol 25 v/v - 🔍	NA	250.0 μl of Ethylene glycol (100 v/v) 750.0 μl of water (100 v/v)
A4	1,4-Dioxane 35 v/v - 🔍	NA	350.0 μl of 1,4-Dioxane (100 v/v) 650.0 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - 2-Propanol 5 v/v - 🔍	NA	50.0 μl of 2-Propanol (100 v/v) 571.4 μl of Ammonium sulfate (3.5 M) 378.6 μl of water (100 v/v)
A6	Imidazole 1 M - 🔍	NA	1000.0 μl of Imidazole pH 7 (1 M)
A7	Polyethylene glycol 1,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 800.0 μl of water (100 v/v)
A8	Sodium chloride 1.5 M - Ethanol 10 v/v - 🔍	NA	100.0 μl of Ethanol (100 v/v) 300.0 μl of Sodium chloride (5 M) 600.0 μl of water (100 v/v)
B1	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
B2	Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 40.0 μl of Sodium chloride (5 M) 560.0 μl of water (100 v/v)
B3	Cobalt (II) chloride 0.01 M - Sodium acetate 0.1 M pH 4.6 1,6-Hexanediol 1 M - 🔍	pH 4.6	166.7 μl of 1,6-Hexanediol (6 M) 10.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 723.3 μl of water (100 v/v)
B4	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 30 v/v - Cadmium chloride 0.1 M - 🔍	pH 4.6	100.0 μl of Cadmium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v)
B5	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	pH 4.6	57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 242.9 μl of water (100 v/v)
B6	Potassium Sodium tartrate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 2 M - 🔍	pH 5.6	571.4 μl of Ammonium sulfate (3.5 M) 133.3 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 195.2 μl of water (100 v/v)
B7	Ammonium sulfate 0.5 M - tri-Sodium citrate 0.1 M pH 5.6 Lithium sulfate 1 M - 🔍	pH 5.6	142.9 μl of Ammonium sulfate (3.5 M) 500.0 μl of Lithium sulfate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 257.1 μl of water (100 v/v)
B8	Sodium chloride 0.5 M - tri-Sodium citrate 0.1 M pH 5.6 Ethylene imine polymer 2 v/v - 🔍	pH 5.6	40.0 μl of Ethylene imine polymer (50 v/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 760.0 μl of water (100 v/v)
C1	tri-Sodium citrate 0.1 M pH 5.6 2-Methyl-2-propanol 35 v/v - 🔍	pH 5.6	875.0 μl of 2-Methyl-2-propanol (40 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 25.0 μl of water (100 v/v)
C2	Iron (III) chloride 0.01 M - tri-Sodium citrate 0.1 M pH 5.6 Jeffamine® M-600 10 v/v - 🔍	pH 5.6	10.0 μl of Iron (III) chloride (1 M) 200.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 690.0 μl of water (100 v/v)
C3	tri-Sodium citrate 0.1 M pH 5.6 1,6-Hexanediol 2.5 M - 🔍	pH 5.6	416.7 μl of 1,6-Hexanediol (6 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 483.3 μl of water (100 v/v)
C4	MES 0.1 M pH 6.5 Magnesium sulfate 1.6 M - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 800.0 μl of Magnesium sulfate (2 M) 100.0 μl of water (100 v/v)
C5	Sodium di-hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 66.7 μl of Potassium di-hydrogen phosphate (1.5 M) 400.0 μl of Sodium chloride (5 M) 25.0 μl of Sodium di-hydrogen phosphate (4 M) 408.3 μl of water (100 v/v)
C6	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 20,000 (30 w/v) 500.0 μl of water (100 v/v)
C7	Ammonium sulfate 1.6 M - MES 0.1 M pH 6.5 1,4-Dioxane 10 v/v - 🔍	pH 6.5	100.0 μl of 1,4-Dioxane (100 v/v) 457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 6.5 (1 M) 342.9 μl of water (100 v/v)
C8	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
D1	Cobalt (II) chloride 0.01 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	514.3 μl of Ammonium sulfate (3.5 M) 10.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 375.7 μl of water (100 v/v)
D2	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 242.9 μl of water (100 v/v)
D3	Zinc sulfate 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 25 v/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 5.0 μl of Zinc sulfate (2 M) 645.0 μl of water (100 v/v)
D4	tri-Sodium citrate 1.6 M - 🔍	NA	1000.0 μl of tri-Sodium citrate (1.6 M)
D5	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 142.9 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 457.1 μl of water (100 v/v)
D6	HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 10 w/v - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 7.5	50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 650.0 μl of water (100 v/v)
D7	HEPES 0.1 M pH 7.5 Jeffamine® M-600 20 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 500.0 μl of water (100 v/v)
D8	Sodium chloride 0.1 M - HEPES 0.1 M pH 7.5 Ammonium sulfate 1.6 M - 🔍	pH 7.5	457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Sodium chloride (5 M) 422.9 μl of water (100 v/v)
E1	HEPES 0.1 M pH 7.5 Ammonium formate 2 M - 🔍	pH 7.5	200.0 μl of Ammonium formate (10 M) 100.0 μl of HEPES pH 7.5 (1 M) 700.0 μl of water (100 v/v)
E2	Cadmium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Sodium acetate 1 M - 🔍	pH 7.5	50.0 μl of Cadmium sulfate (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 333.3 μl of Sodium acetate (3 M) 516.7 μl of water (100 v/v)
E3	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 70 v/v - 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v)
E4	HEPES 0.1 M pH 7.5 Sodium chloride 4.3 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 860.0 μl of Sodium chloride (5 M) 40.0 μl of water (100 v/v)
E5	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 10 w/v - Ethylene glycol 8 v/v - 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v)
E6	HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v)
E7	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 1,6-Hexanediol 3.4 M - 🔍	pH 8.5	566.7 μl of 1,6-Hexanediol (6 M) 100.0 μl of Magnesium chloride (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v)
E8	tris chloride BCC 0.1 M pH 8.5 2-Methyl-2-propanol 25 v/v - 🔍	pH 8.5	625.0 μl of 2-Methyl-2-propanol (40 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 275.0 μl of water (100 v/v)
F1	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1 M - 🔍	pH 8.5	500.0 μl of Lithium sulfate (2 M) 2.5 μl of Nickel (II) chloride (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
F2	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Glycerol 12 v/v - 🔍	pH 8.5	428.6 μl of Ammonium sulfate (3.5 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 351.4 μl of water (100 v/v)
F3	Ammonium di-hydrogen phosphate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
F4	tris chloride BCC 0.1 M pH 8.5 Ethanol 20 v/v - 🔍	pH 8.5	200.0 μl of Ethanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v)
F5	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 2,000 20 w/v - 🔍	pH 8.5	2.5 μl of Nickel (II) chloride (4 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 497.5 μl of water (100 v/v)
F6	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 20 v/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 20.0 μl of Sodium chloride (5 M) 680.0 μl of water (100 v/v)
F7	BICINE 0.1 M pH 9 Magnesium chloride 2 M - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Magnesium chloride (5 M) 500.0 μl of water (100 v/v)
F8	BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - Polyethylene glycol 20,000 10 w/v - 🔍	pH 9	20.0 μl of 1,4-Dioxane (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 546.7 μl of water (100 v/v)