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Supplemental Information

Screen Description

Crystal Screen 2

Hampton Research / Crystal Screen 2
Created 2009-11-16.

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Crystal Screen 2

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Crystal Screen 2

    Screen Structure

    Crystal Screen 2

    Well Count 48
    Number of distinct ingredients 46
    Internal diversity (score mean dist) 0.950

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:38:29.114926 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Crystal Screen 2
    Buffer 46
    Cryoprotectant 1
    Detergent 1
    Organic (non-volatile) 17
    Organic (volatile) 6
    Polymer 17
    Salt 53

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Crystal Screen 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    400 μl of water (100 v/v)
    A2
    • Sodium chloride 0.5 M -
    • Magnesium chloride 0.01 M -
    • Cetyltrimethylammonium bromide 0.01 mM -
    		NA
    0 μl of Cetyltrimethylammonium bromide (50 mM)
    100 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    894 μl of water (100 v/v)
    A3
    • Ethylene glycol 25 v/v -
    		NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    A4
    • 1,4-Dioxane 35 v/v -
    		NA
    350 μl of 1,4-Dioxane (100 v/v)
    650 μl of water (100 v/v)
    A5
    • Ammonium sulfate 2 M -
    • 2-Propanol 5 v/v -
    		NA
    571 μl of Ammonium sulfate (3.5 M)
    50 μl of 2-Propanol (100 v/v)
    378 μl of water (100 v/v)
    A6
    • Imidazole 1 M -
    		NA
    1000 μl of Imidazole pH 7 (1 M)
    A7
    • Polyethylene glycol 1,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    800 μl of water (100 v/v)
    A8
    • Sodium chloride 1.5 M -
    • Ethanol 10 v/v -
    		NA
    100 μl of Ethanol (100 v/v)
    300 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    B1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    B2
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Sodium chloride (5 M)
    560 μl of water (100 v/v)
    B3
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.1 M pH 4.6
    • 1,6-Hexanediol 1 M -
    		pH 4.6
    10 μl of Cobalt (II) chloride (1 M)
    166 μl of 1,6-Hexanediol (6 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    723 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.1 M -
    		pH 4.6
    100 μl of Cadmium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    		pH 4.6
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    242 μl of water (100 v/v)
    B6
    • Potassium Sodium tartrate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    133 μl of Potassium Sodium tartrate (1.5 M)
    195 μl of water (100 v/v)
    B7
    • Ammonium sulfate 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Lithium sulfate 1 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of Lithium sulfate (2 M)
    142 μl of Ammonium sulfate (3.5 M)
    257 μl of water (100 v/v)
    B8
    • Sodium chloride 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ethylene imine polymer 2 v/v -
    		pH 5.6
    40 μl of Ethylene imine polymer (50 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of Sodium chloride (5 M)
    760 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Methyl-2-propanol 35 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    875 μl of 2-Methyl-2-propanol (40 v/v)
    25 μl of water (100 v/v)
    C2
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    10 μl of Iron (III) chloride (1 M)
    200 μl of Jeffamine® M-600 pH 7 (50 v/v)
    690 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.5 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    416 μl of 1,6-Hexanediol (6 M)
    483 μl of water (100 v/v)
    C4
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    		pH 6.5
    800 μl of Magnesium sulfate (2 M)
    100 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    C5
    • Sodium di-hydrogen phosphate 0.1 M -
    • MES 0.1 M pH 6.5
    • Sodium chloride 2 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    		pH 6.5
    25 μl of Sodium di-hydrogen phosphate (4 M)
    66 μl of Potassium di-hydrogen phosphate (1.5 M)
    400 μl of Sodium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    408 μl of water (100 v/v)
    C6
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    		pH 6.5
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    C7
    • Ammonium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    • 1,4-Dioxane 10 v/v -
    		pH 6.5
    457 μl of Ammonium sulfate (3.5 M)
    100 μl of 1,4-Dioxane (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    342 μl of water (100 v/v)
    C8
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    		pH 6.5
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    D1
    • Cobalt (II) chloride 0.01 M -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    		pH 6.5
    10 μl of Cobalt (II) chloride (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    100 μl of MES pH 6.5 (1 M)
    375 μl of water (100 v/v)
    D2
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    		pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of MES pH 6.5 (1 M)
    242 μl of water (100 v/v)
    D3
    • Zinc sulfate 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    		pH 6.5
    5 μl of Zinc sulfate (2 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    645 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 1.6 M -
    		NA
    1000 μl of tri-Sodium citrate (1.6 M)
    D5
    • Ammonium sulfate 0.5 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    142 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    457 μl of water (100 v/v)
    D6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 10 w/v -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    		pH 7.5
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    D7
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Jeffamine® M-600 pH 7 (50 v/v)
    500 μl of water (100 v/v)
    D8
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    		pH 7.5
    457 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    422 μl of water (100 v/v)
    E1
    • HEPES 0.1 M pH 7.5
    • Ammonium formate 2 M -
    		pH 7.5
    200 μl of Ammonium formate (10 M)
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    E2
    • Cadmium sulfate 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of Sodium acetate (3 M)
    50 μl of Cadmium sulfate (1 M)
    516 μl of water (100 v/v)
    E3
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    		pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 4.3 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    860 μl of Sodium chloride (5 M)
    40 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 8 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    620 μl of water (100 v/v)
    E6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    		pH 7.5
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 M -
    		pH 8.5
    566 μl of 1,6-Hexanediol (6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    233 μl of water (100 v/v)
    E8
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Methyl-2-propanol 25 v/v -
    		pH 8.5
    625 μl of 2-Methyl-2-propanol (40 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    275 μl of water (100 v/v)
    F1
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    		pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    500 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    397 μl of water (100 v/v)
    F2
    • Ammonium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 12 v/v -
    		pH 8.5
    428 μl of Ammonium sulfate (3.5 M)
    120 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    351 μl of water (100 v/v)
    F3
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    		pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ethanol (100 v/v)
    700 μl of water (100 v/v)
    F5
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    		pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    497 μl of water (100 v/v)
    F6
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    		pH 9
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    20 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    F7
    • BICINE 0.1 M pH 9
    • Magnesium chloride 2 M -
    		pH 9
    400 μl of Magnesium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    F8
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    • Polyethylene glycol 20,000 10 w/v -
    		pH 9
    20 μl of 1,4-Dioxane (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    546 μl of water (100 v/v)