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Supplemental Information

Screen Description

Crystal Screen Cryo

Hampton Research / Crystal Screen Cryo
Created 2009-11-16.

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Crystal Screen Cryo

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Crystal Screen Cryo

    Screen Structure

    Crystal Screen Cryo

    Well Count 96
    Number of distinct ingredients 29
    Internal diversity (score mean dist) 0.824

    Screen Composition

    More information about screen composition chart.
    2025-06-14T14:12:49.075926 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Crystal Screen Cryo
    Buffer 62
    Cryoprotectant 44
    Organic (non-volatile) 52
    Organic (volatile) 7
    Polymer 25
    Salt 65

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Crystal Screen Cryo Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    10 μl of Calcium chloride (2 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    590 μl of water (100 v/v)
    A2
    • Potassium Sodium tartrate 0.26 M -
    • Glycerol 35 v/v -
    		NA
    173 μl of Potassium Sodium tartrate (1.5 M)
    350 μl of Glycerol (100 v/v)
    476 μl of water (100 v/v)
    A3
    • Ammonium di-hydrogen phosphate 0.26 M -
    • Glycerol 35 v/v -
    		NA
    104 μl of Ammonium di-hydrogen phosphate (2.5 M)
    350 μl of Glycerol (100 v/v)
    546 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.075 M pH 8.5
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    		pH 8.5
    75 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of Glycerol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    246 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    475 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.16 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Polyethylene glycol 4,000 24 w/v -
    • Glycerol 20 v/v -
    		pH 8.5
    80 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2 M)
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    160 μl of water (100 v/v)
    A7
    • Sodium cacodylate 0.07 M pH 6.5
    • Sodium acetate 0.98 M -
    • Glycerol 30 v/v -
    		pH 6.5
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium cacodylate pH 6.5 (1 M)
    326 μl of Sodium acetate (3 M)
    303 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.14 M -
    • Sodium cacodylate 0.07 M pH 6.5
    • 2-Propanol 21 v/v -
    • Glycerol 30 v/v -
    		pH 6.5
    300 μl of Glycerol (100 v/v)
    210 μl of 2-Propanol (100 v/v)
    87 μl of tri-Sodium citrate (1.6 M)
    70 μl of Sodium cacodylate pH 6.5 (1 M)
    332 μl of water (100 v/v)
    A9
    • Ammonium acetate 0.17 M -
    • tri-Sodium citrate 0.085 M pH 5.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 5.6
    150 μl of Glycerol (100 v/v)
    85 μl of tri-Sodium citrate pH 5.6 (1 M)
    170 μl of Ammonium acetate (1 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of water (100 v/v)
    A10
    • Ammonium acetate 0.17 M -
    • Sodium acetate 0.085 M pH 4.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 4.6
    150 μl of Glycerol (100 v/v)
    170 μl of Ammonium acetate (1 M)
    85 μl of Sodium acetate pH 4.6 (1 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of water (100 v/v)
    A11
    • tri-Sodium citrate 0.07 M pH 5.6
    • Ammonium di-hydrogen phosphate 0.7 M -
    • Glycerol 30 v/v -
    		pH 5.6
    280 μl of Ammonium di-hydrogen phosphate (2.5 M)
    300 μl of Glycerol (100 v/v)
    70 μl of tri-Sodium citrate pH 5.6 (1 M)
    350 μl of water (100 v/v)
    A12
    • Magnesium chloride 0.18 M -
    • HEPES 0.09 M pH 7.5
    • 2-Propanol 27 v/v -
    • Glycerol 10 v/v -
    		pH 7.5
    90 μl of Magnesium chloride (2 M)
    100 μl of Glycerol (100 v/v)
    270 μl of 2-Propanol (100 v/v)
    90 μl of HEPES pH 7.5 (1 M)
    450 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    475 μl of water (100 v/v)
    B2
    • Calcium chloride 0.19 M -
    • HEPES 0.095 M pH 7.5
    • Polyethylene glycol 400 26.6 v/v -
    • Glycerol 5 v/v -
    		pH 7.5
    95 μl of Calcium chloride (2 M)
    50 μl of Glycerol (100 v/v)
    95 μl of HEPES pH 7.5 (1 M)
    266 μl of Polyethylene glycol 400 (100 v/v)
    494 μl of water (100 v/v)
    B3
    • Ammonium sulfate 0.17 M -
    • Sodium cacodylate 0.085 M pH 6.5
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 6.5
    150 μl of Glycerol (100 v/v)
    85 μl of Sodium cacodylate pH 6.5 (1 M)
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    48 μl of Ammonium sulfate (3.5 M)
    206 μl of water (100 v/v)
    B4
    • HEPES 0.075 M pH 7.5
    • Lithium sulfate 1.12 M -
    • Glycerol 25 v/v -
    		pH 7.5
    562 μl of Lithium sulfate (2 M)
    250 μl of Glycerol (100 v/v)
    75 μl of HEPES pH 7.5 (1 M)
    112 μl of water (100 v/v)
    B5
    • Lithium sulfate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 8.5
    85 μl of tris chloride BCC pH 8.5 (1 M)
    85 μl of Lithium sulfate (2 M)
    150 μl of Glycerol (100 v/v)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    170 μl of water (100 v/v)
    B6
    • Magnesium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    		pH 6.5
    200 μl of Glycerol (100 v/v)
    160 μl of Magnesium acetate (1 M)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    240 μl of water (100 v/v)
    B7
    • Ammonium acetate 0.16 M -
    • tris chloride BCC 0.08 M pH 8.5
    • 2-Propanol 24 v/v -
    • Glycerol 20 v/v -
    		pH 8.5
    80 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Glycerol (100 v/v)
    240 μl of 2-Propanol (100 v/v)
    160 μl of Ammonium acetate (1 M)
    320 μl of water (100 v/v)
    B8
    • Ammonium sulfate 0.16 M -
    • Sodium acetate 0.08 M pH 4.6
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 20 v/v -
    		pH 4.6
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    45 μl of Ammonium sulfate (3.5 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    274 μl of water (100 v/v)
    B9
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 6.5
    200 μl of Magnesium acetate (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B10
    • Sodium acetate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 8.5
    85 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Glycerol (100 v/v)
    56 μl of Sodium acetate (3 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    198 μl of water (100 v/v)
    B11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    100 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    B12
    • Calcium chloride 0.14 M -
    • Sodium acetate 0.07 M pH 4.6
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    		pH 4.6
    70 μl of Calcium chloride (2 M)
    300 μl of Glycerol (100 v/v)
    140 μl of 2-Propanol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    420 μl of water (100 v/v)
    C1
    • Imidazole 0.07 M pH 6.5
    • Sodium acetate 0.7 M -
    • Glycerol 30 v/v -
    		pH 6.5
    300 μl of Glycerol (100 v/v)
    70 μl of Imidazole pH 6.5 (1 M)
    233 μl of Sodium acetate (3 M)
    396 μl of water (100 v/v)
    C2
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium acetate (1 M)
    400 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.14 M -
    • HEPES 0.07 M pH 7.5
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    		pH 7.5
    300 μl of Glycerol (100 v/v)
    140 μl of 2-Propanol (100 v/v)
    87 μl of tri-Sodium citrate (1.6 M)
    70 μl of HEPES pH 7.5 (1 M)
    402 μl of water (100 v/v)
    C4
    • Sodium acetate 0.17 M -
    • Sodium cacodylate 0.085 M pH 6.5
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 6.5
    150 μl of Glycerol (100 v/v)
    85 μl of Sodium cacodylate pH 6.5 (1 M)
    56 μl of Sodium acetate (3 M)
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    198 μl of water (100 v/v)
    C5
    • HEPES 0.065 M pH 7.5
    • Potassium Sodium tartrate 0.52 M -
    • Glycerol 35 v/v -
    		pH 7.5
    346 μl of Potassium Sodium tartrate (1.5 M)
    350 μl of Glycerol (100 v/v)
    65 μl of HEPES pH 7.5 (1 M)
    238 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    		NA
    150 μl of Glycerol (100 v/v)
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    48 μl of Ammonium sulfate (3.5 M)
    291 μl of water (100 v/v)
    C7
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		NA
    150 μl of Glycerol (100 v/v)
    48 μl of Ammonium sulfate (3.5 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    291 μl of water (100 v/v)
    C8
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    		NA
    250 μl of Glycerol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    321 μl of water (100 v/v)
    C9
    • Sodium formate 3.6 M -
    • Glycerol 10 v/v -
    		NA
    514 μl of Sodium formate (7 M)
    100 μl of Glycerol (100 v/v)
    385 μl of water (100 v/v)
    C10
    • Sodium formate 1.4 M -
    • Sodium acetate 0.07 M pH 4.6
    • Glycerol 30 v/v -
    		pH 4.6
    200 μl of Sodium formate (7 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    430 μl of water (100 v/v)
    C11
    • HEPES 0.075 M pH 7.5
    • Sodium di-hydrogen phosphate 0.6 M -
    • Potassium di-hydrogen phosphate 0.6 M -
    • Glycerol 25 v/v -
    		pH 7.5
    250 μl of Glycerol (100 v/v)
    75 μl of HEPES pH 7.5 (1 M)
    400 μl of Potassium di-hydrogen phosphate (1.5 M)
    150 μl of Sodium di-hydrogen phosphate (4 M)
    125 μl of water (100 v/v)
    C12
    • tris chloride BCC 0.065 M pH 8.5
    • Polyethylene glycol 8,000 5.2 w/v -
    • Glycerol 35 v/v -
    		pH 8.5
    65 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of Glycerol (100 v/v)
    104 μl of Polyethylene glycol 8,000 (50 w/v)
    481 μl of water (100 v/v)
    D1
    • Sodium acetate 0.07 M pH 4.6
    • Polyethylene glycol 4,000 5.6 w/v -
    • Glycerol 30 v/v -
    		pH 4.6
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    112 μl of Polyethylene glycol 4,000 (50 w/v)
    518 μl of water (100 v/v)
    D2
    • HEPES 0.09 M pH 7.5
    • tri-Sodium citrate 1.26 M -
    • Glycerol 10 v/v -
    		pH 7.5
    100 μl of Glycerol (100 v/v)
    787 μl of tri-Sodium citrate (1.6 M)
    90 μl of HEPES pH 7.5 (1 M)
    22 μl of water (100 v/v)
    D3
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 400 1.7 v/v -
    • Ammonium sulfate 1.7 M -
    • Glycerol 15 v/v -
    		pH 7.5
    150 μl of Glycerol (100 v/v)
    85 μl of HEPES pH 7.5 (1 M)
    485 μl of Ammonium sulfate (3.5 M)
    17 μl of Polyethylene glycol 400 (100 v/v)
    262 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.095 M pH 5.6
    • 2-Propanol 19 v/v -
    • Polyethylene glycol 4,000 19 w/v -
    • Glycerol 5 v/v -
    		pH 5.6
    50 μl of Glycerol (100 v/v)
    95 μl of tri-Sodium citrate pH 5.6 (1 M)
    190 μl of 2-Propanol (100 v/v)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    285 μl of water (100 v/v)
    D5
    • HEPES 0.085 M pH 7.5
    • 2-Propanol 8.5 v/v -
    • Polyethylene glycol 4,000 17 w/v -
    • Glycerol 15 v/v -
    		pH 7.5
    150 μl of Glycerol (100 v/v)
    85 μl of 2-Propanol (100 v/v)
    85 μl of HEPES pH 7.5 (1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    D6
    • Potassium di-hydrogen phosphate 0.04 M -
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    26 μl of Potassium di-hydrogen phosphate (1.5 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    453 μl of water (100 v/v)
    D7
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    		NA
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Glycerol (100 v/v)
    320 μl of water (100 v/v)
    D8
    • Magnesium formate 0.1 M -
    • Glycerol 50 v/v -
    		NA
    500 μl of Glycerol (100 v/v)
    100 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    D9
    • Zinc acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    		pH 6.5
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Zinc acetate (1 M)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    272 μl of water (100 v/v)
    D10
    • Calcium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    		pH 6.5
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Calcium acetate (1 M)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    272 μl of water (100 v/v)
    D11
    • Ammonium sulfate 1.6 M -
    • Sodium acetate 0.08 M pH 4.6
    • Glycerol 20 v/v -
    		pH 4.6
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    262 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.08 M pH 8.5
    • Ammonium di-hydrogen phosphate 1.6 M -
    • Glycerol 20 v/v -
    		pH 8.5
    80 μl of tris chloride BCC pH 8.5 (1 M)
    640 μl of Ammonium di-hydrogen phosphate (2.5 M)
    200 μl of Glycerol (100 v/v)
    80 μl of water (100 v/v)
    E1
    • Lithium sulfate 0.8 M -
    • Polyethylene glycol 8,000 1.6 w/v -
    • Glycerol 20 v/v -
    		NA
    400 μl of Lithium sulfate (2 M)
    200 μl of Glycerol (100 v/v)
    32 μl of Polyethylene glycol 8,000 (50 w/v)
    368 μl of water (100 v/v)
    E2
    • Lithium sulfate 0.4 M -
    • Polyethylene glycol 8,000 12 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Lithium sulfate (2 M)
    200 μl of Glycerol (100 v/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    360 μl of water (100 v/v)
    E3NA
    check warnings
    E4NA
    check warnings
    E5NA
    check warnings
    E6NA
    check warnings
    E7NA
    check warnings
    E8NA
    check warnings
    E9NA
    check warnings
    E10NA
    check warnings
    E11NA
    check warnings
    E12NA
    check warnings
    F1NA
    check warnings
    F2NA
    check warnings
    F3NA
    check warnings
    F4NA
    check warnings
    F5NA
    check warnings
    F6NA
    check warnings
    F7NA
    check warnings
    F8NA
    check warnings
    F9NA
    check warnings
    F10NA
    check warnings
    F11NA
    check warnings
    F12NA
    check warnings
    G1NA
    check warnings
    G2NA
    check warnings
    G3NA
    check warnings
    G4NA
    check warnings
    G5NA
    check warnings
    G6NA
    check warnings
    G7NA
    check warnings
    G8NA
    check warnings
    G9NA
    check warnings
    G10NA
    check warnings
    G11NA
    check warnings
    G12NA
    check warnings
    H1NA
    check warnings
    H2NA
    check warnings
    H3NA
    check warnings
    H4NA
    check warnings
    H5NA
    check warnings
    H6NA
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    H7NA
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    H8NA
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    H9NA
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    H10NA
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    H11NA
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    H12NA
    check warnings