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Supplemental Information

Screen Description

Crystal Screen Cryo

Hampton Research / Crystal Screen Cryo
Created 2009-11-16.

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Crystal Screen Cryo

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Crystal Screen Cryo

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Screen Structure

Crystal Screen Cryo

Well Count 96
Number of distinct ingredients 29
Internal diversity (score mean dist) 0.824

Screen Composition

More information about screen composition chart.
2025-08-11T20:34:49.696308 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Crystal Screen Cryo
Buffer 62
Cryoprotectant 44
Organic (non-volatile) 52
Organic (volatile) 7
Polymer 25
Salt 65

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Crystal Screen Cryo Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
A2
  • Potassium Sodium tartrate 0.26 M -
  • Glycerol 35 v/v -
NA
350.0 μl of Glycerol (100 v/v)
173.3 μl of Potassium Sodium tartrate (1.5 M)
476.7 μl of water (100 v/v)
A3
  • Ammonium di-hydrogen phosphate 0.26 M -
  • Glycerol 35 v/v -
NA
104.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
350.0 μl of Glycerol (100 v/v)
546.0 μl of water (100 v/v)
A4
  • tris chloride BCC 0.075 M pH 8.5
  • Ammonium sulfate 1.5 M -
  • Glycerol 25 v/v -
pH 8.5
428.6 μl of Ammonium sulfate (3.5 M)
250.0 μl of Glycerol (100 v/v)
75.0 μl of tris chloride BCC pH 8.5 (1 M)
246.4 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A6
  • Magnesium chloride 0.16 M -
  • tris chloride BCC 0.08 M pH 8.5
  • Polyethylene glycol 4,000 24 w/v -
  • Glycerol 20 v/v -
pH 8.5
200.0 μl of Glycerol (100 v/v)
80.0 μl of Magnesium chloride (2 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
160.0 μl of water (100 v/v)
A7
  • Sodium cacodylate 0.07 M pH 6.5
  • Sodium acetate 0.98 M -
  • Glycerol 30 v/v -
pH 6.5
300.0 μl of Glycerol (100 v/v)
326.7 μl of Sodium acetate (3 M)
70.0 μl of Sodium cacodylate pH 6.5 (1 M)
303.3 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.14 M -
  • Sodium cacodylate 0.07 M pH 6.5
  • 2-Propanol 21 v/v -
  • Glycerol 30 v/v -
pH 6.5
210.0 μl of 2-Propanol (100 v/v)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium cacodylate pH 6.5 (1 M)
87.5 μl of tri-Sodium citrate (1.6 M)
332.5 μl of water (100 v/v)
A9
  • Ammonium acetate 0.17 M -
  • tri-Sodium citrate 0.085 M pH 5.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 5.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
85.0 μl of water (100 v/v)
A10
  • Ammonium acetate 0.17 M -
  • Sodium acetate 0.085 M pH 4.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 4.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate pH 4.6 (1 M)
85.0 μl of water (100 v/v)
A11
  • tri-Sodium citrate 0.07 M pH 5.6
  • Ammonium di-hydrogen phosphate 0.7 M -
  • Glycerol 30 v/v -
pH 5.6
280.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of tri-Sodium citrate pH 5.6 (1 M)
350.0 μl of water (100 v/v)
A12
  • Magnesium chloride 0.18 M -
  • HEPES 0.09 M pH 7.5
  • 2-Propanol 27 v/v -
  • Glycerol 10 v/v -
pH 7.5
270.0 μl of 2-Propanol (100 v/v)
100.0 μl of Glycerol (100 v/v)
90.0 μl of HEPES pH 7.5 (1 M)
90.0 μl of Magnesium chloride (2 M)
450.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
B2
  • Calcium chloride 0.19 M -
  • HEPES 0.095 M pH 7.5
  • Polyethylene glycol 400 26.6 v/v -
  • Glycerol 5 v/v -
pH 7.5
95.0 μl of Calcium chloride (2 M)
50.0 μl of Glycerol (100 v/v)
95.0 μl of HEPES pH 7.5 (1 M)
266.0 μl of Polyethylene glycol 400 (100 v/v)
494.0 μl of water (100 v/v)
B3
  • Ammonium sulfate 0.17 M -
  • Sodium cacodylate 0.085 M pH 6.5
  • Polyethylene glycol 8,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 6.5
48.6 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 8,000 (50 w/v)
85.0 μl of Sodium cacodylate pH 6.5 (1 M)
206.4 μl of water (100 v/v)
B4
  • HEPES 0.075 M pH 7.5
  • Lithium sulfate 1.12 M -
  • Glycerol 25 v/v -
pH 7.5
250.0 μl of Glycerol (100 v/v)
75.0 μl of HEPES pH 7.5 (1 M)
562.5 μl of Lithium sulfate (2 M)
112.5 μl of water (100 v/v)
B5
  • Lithium sulfate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 8.5
150.0 μl of Glycerol (100 v/v)
85.0 μl of Lithium sulfate (2 M)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
170.0 μl of water (100 v/v)
B6
  • Magnesium acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
pH 6.5
200.0 μl of Glycerol (100 v/v)
160.0 μl of Magnesium acetate (1 M)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
240.0 μl of water (100 v/v)
B7
  • Ammonium acetate 0.16 M -
  • tris chloride BCC 0.08 M pH 8.5
  • 2-Propanol 24 v/v -
  • Glycerol 20 v/v -
pH 8.5
240.0 μl of 2-Propanol (100 v/v)
160.0 μl of Ammonium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B8
  • Ammonium sulfate 0.16 M -
  • Sodium acetate 0.08 M pH 4.6
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 20 v/v -
pH 4.6
45.7 μl of Ammonium sulfate (3.5 M)
200.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
274.3 μl of water (100 v/v)
B9
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Magnesium acetate (1 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B10
  • Sodium acetate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
56.7 μl of Sodium acetate (3 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
198.3 μl of water (100 v/v)
B11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
B12
  • Calcium chloride 0.14 M -
  • Sodium acetate 0.07 M pH 4.6
  • 2-Propanol 14 v/v -
  • Glycerol 30 v/v -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
70.0 μl of Calcium chloride (2 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
420.0 μl of water (100 v/v)
C1
  • Imidazole 0.07 M pH 6.5
  • Sodium acetate 0.7 M -
  • Glycerol 30 v/v -
pH 6.5
300.0 μl of Glycerol (100 v/v)
70.0 μl of Imidazole pH 6.5 (1 M)
233.3 μl of Sodium acetate (3 M)
396.7 μl of water (100 v/v)
C2
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
C3
  • tri-Sodium citrate 0.14 M -
  • HEPES 0.07 M pH 7.5
  • 2-Propanol 14 v/v -
  • Glycerol 30 v/v -
pH 7.5
140.0 μl of 2-Propanol (100 v/v)
300.0 μl of Glycerol (100 v/v)
70.0 μl of HEPES pH 7.5 (1 M)
87.5 μl of tri-Sodium citrate (1.6 M)
402.5 μl of water (100 v/v)
C4
  • Sodium acetate 0.17 M -
  • Sodium cacodylate 0.085 M pH 6.5
  • Polyethylene glycol 8,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 6.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 8,000 (50 w/v)
56.7 μl of Sodium acetate (3 M)
85.0 μl of Sodium cacodylate pH 6.5 (1 M)
198.3 μl of water (100 v/v)
C5
  • HEPES 0.065 M pH 7.5
  • Potassium Sodium tartrate 0.52 M -
  • Glycerol 35 v/v -
pH 7.5
350.0 μl of Glycerol (100 v/v)
65.0 μl of HEPES pH 7.5 (1 M)
346.7 μl of Potassium Sodium tartrate (1.5 M)
238.3 μl of water (100 v/v)
C6
  • Ammonium sulfate 0.17 M -
  • Polyethylene glycol 8,000 25.5 w/v -
  • Glycerol 15 v/v -
NA
48.6 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 8,000 (50 w/v)
291.4 μl of water (100 v/v)
C7
  • Ammonium sulfate 0.17 M -
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
NA
48.6 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
291.4 μl of water (100 v/v)
C8
  • Ammonium sulfate 1.5 M -
  • Glycerol 25 v/v -
NA
428.6 μl of Ammonium sulfate (3.5 M)
250.0 μl of Glycerol (100 v/v)
321.4 μl of water (100 v/v)
C9
  • Sodium formate 3.6 M -
  • Glycerol 10 v/v -
NA
100.0 μl of Glycerol (100 v/v)
514.3 μl of Sodium formate (7 M)
385.7 μl of water (100 v/v)
C10
  • Sodium formate 1.4 M -
  • Sodium acetate 0.07 M pH 4.6
  • Glycerol 30 v/v -
pH 4.6
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
200.0 μl of Sodium formate (7 M)
430.0 μl of water (100 v/v)
C11
  • HEPES 0.075 M pH 7.5
  • Sodium di-hydrogen phosphate 0.6 M -
  • Potassium di-hydrogen phosphate 0.6 M -
  • Glycerol 25 v/v -
pH 7.5
250.0 μl of Glycerol (100 v/v)
75.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium di-hydrogen phosphate (1.5 M)
150.0 μl of Sodium di-hydrogen phosphate (4 M)
125.0 μl of water (100 v/v)
C12
  • tris chloride BCC 0.065 M pH 8.5
  • Polyethylene glycol 8,000 5.2 w/v -
  • Glycerol 35 v/v -
pH 8.5
350.0 μl of Glycerol (100 v/v)
104.0 μl of Polyethylene glycol 8,000 (50 w/v)
65.0 μl of tris chloride BCC pH 8.5 (1 M)
481.0 μl of water (100 v/v)
D1
  • Sodium acetate 0.07 M pH 4.6
  • Polyethylene glycol 4,000 5.6 w/v -
  • Glycerol 30 v/v -
pH 4.6
300.0 μl of Glycerol (100 v/v)
112.0 μl of Polyethylene glycol 4,000 (50 w/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
518.0 μl of water (100 v/v)
D2
  • HEPES 0.09 M pH 7.5
  • tri-Sodium citrate 1.26 M -
  • Glycerol 10 v/v -
pH 7.5
100.0 μl of Glycerol (100 v/v)
90.0 μl of HEPES pH 7.5 (1 M)
787.5 μl of tri-Sodium citrate (1.6 M)
22.5 μl of water (100 v/v)
D3
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 400 1.7 v/v -
  • Ammonium sulfate 1.7 M -
  • Glycerol 15 v/v -
pH 7.5
485.7 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
17.0 μl of Polyethylene glycol 400 (100 v/v)
262.3 μl of water (100 v/v)
D4
  • tri-Sodium citrate 0.095 M pH 5.6
  • 2-Propanol 19 v/v -
  • Polyethylene glycol 4,000 19 w/v -
  • Glycerol 5 v/v -
pH 5.6
190.0 μl of 2-Propanol (100 v/v)
50.0 μl of Glycerol (100 v/v)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
95.0 μl of tri-Sodium citrate pH 5.6 (1 M)
285.0 μl of water (100 v/v)
D5
  • HEPES 0.085 M pH 7.5
  • 2-Propanol 8.5 v/v -
  • Polyethylene glycol 4,000 17 w/v -
  • Glycerol 15 v/v -
pH 7.5
85.0 μl of 2-Propanol (100 v/v)
150.0 μl of Glycerol (100 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)
D6
  • Potassium di-hydrogen phosphate 0.04 M -
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
26.7 μl of Potassium di-hydrogen phosphate (1.5 M)
453.3 μl of water (100 v/v)
D7
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
D8
  • Magnesium formate 0.1 M -
  • Glycerol 50 v/v -
NA
500.0 μl of Glycerol (100 v/v)
100.0 μl of Magnesium formate (1 M)
400.0 μl of water (100 v/v)
D9
  • Zinc acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
pH 6.5
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
160.0 μl of Zinc acetate (1 M)
272.0 μl of water (100 v/v)
D10
  • Calcium acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
272.0 μl of water (100 v/v)
D11
  • Ammonium sulfate 1.6 M -
  • Sodium acetate 0.08 M pH 4.6
  • Glycerol 20 v/v -
pH 4.6
457.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
262.9 μl of water (100 v/v)
D12
  • tris chloride BCC 0.08 M pH 8.5
  • Ammonium di-hydrogen phosphate 1.6 M -
  • Glycerol 20 v/v -
pH 8.5
640.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
80.0 μl of water (100 v/v)
E1
  • Lithium sulfate 0.8 M -
  • Polyethylene glycol 8,000 1.6 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
400.0 μl of Lithium sulfate (2 M)
32.0 μl of Polyethylene glycol 8,000 (50 w/v)
368.0 μl of water (100 v/v)
E2
  • Lithium sulfate 0.4 M -
  • Polyethylene glycol 8,000 12 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
200.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
360.0 μl of water (100 v/v)
E3NA
1000.0 μl of water (100 v/v)
E4NA
1000.0 μl of water (100 v/v)
E5NA
1000.0 μl of water (100 v/v)
E6NA
1000.0 μl of water (100 v/v)
E7NA
1000.0 μl of water (100 v/v)
E8NA
1000.0 μl of water (100 v/v)
E9NA
1000.0 μl of water (100 v/v)
E10NA
1000.0 μl of water (100 v/v)
E11NA
1000.0 μl of water (100 v/v)
E12NA
1000.0 μl of water (100 v/v)
F1NA
1000.0 μl of water (100 v/v)
F2NA
1000.0 μl of water (100 v/v)
F3NA
1000.0 μl of water (100 v/v)
F4NA
1000.0 μl of water (100 v/v)
F5NA
1000.0 μl of water (100 v/v)
F6NA
1000.0 μl of water (100 v/v)
F7NA
1000.0 μl of water (100 v/v)
F8NA
1000.0 μl of water (100 v/v)
F9NA
1000.0 μl of water (100 v/v)
F10NA
1000.0 μl of water (100 v/v)
F11NA
1000.0 μl of water (100 v/v)
F12NA
1000.0 μl of water (100 v/v)
G1NA
1000.0 μl of water (100 v/v)
G2NA
1000.0 μl of water (100 v/v)
G3NA
1000.0 μl of water (100 v/v)
G4NA
1000.0 μl of water (100 v/v)
G5NA
1000.0 μl of water (100 v/v)
G6NA
1000.0 μl of water (100 v/v)
G7NA
1000.0 μl of water (100 v/v)
G8NA
1000.0 μl of water (100 v/v)
G9NA
1000.0 μl of water (100 v/v)
G10NA
1000.0 μl of water (100 v/v)
G11NA
1000.0 μl of water (100 v/v)
G12NA
1000.0 μl of water (100 v/v)
H1NA
1000.0 μl of water (100 v/v)
H2NA
1000.0 μl of water (100 v/v)
H3NA
1000.0 μl of water (100 v/v)
H4NA
1000.0 μl of water (100 v/v)
H5NA
1000.0 μl of water (100 v/v)
H6NA
1000.0 μl of water (100 v/v)
H7NA
1000.0 μl of water (100 v/v)
H8NA
1000.0 μl of water (100 v/v)
H9NA
1000.0 μl of water (100 v/v)
H10NA
1000.0 μl of water (100 v/v)
H11NA
1000.0 μl of water (100 v/v)
H12NA
1000.0 μl of water (100 v/v)