C6 - Crystal Screen Cryo - Screen Contents

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Supplemental Information

Screen Description

Hampton Research / Crystal Screen Cryo

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	96
Number of distinct ingredients	29
Internal diversity (score mean dist)	0.83

Screen Composition

More information about screen composition chart.

ingredient type	Crystal Screen Cryo
Buffer	62
Cryoprotectant	44
Organic (non-volatile)	52
Organic (volatile)	7
Polymer	25
Salt	65

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Crystal Screen Cryo Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 590.0 μl of water (100 v/v)
A2	Potassium Sodium tartrate 0.26 M - Glycerol 35 v/v - 🔍	NA	350.0 μl of Glycerol (100 v/v) 173.33 μl of Potassium Sodium tartrate (1.5 M) 476.67 μl of water (100 v/v)
A3	Ammonium di-hydrogen phosphate 0.26 M - Glycerol 35 v/v - 🔍	NA	104.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 350.0 μl of Glycerol (100 v/v) 546.0 μl of water (100 v/v)
A4	tris chloride BCC 0.075 M pH 8.5 Ammonium sulfate 1.5 M - Glycerol 25 v/v - 🔍	pH 8.5	428.57 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 246.43 μl of water (100 v/v)
A5	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 125.0 μl of tri-Sodium citrate (1.6 M) 475.0 μl of water (100 v/v)
A6	Magnesium chloride 0.16 M - tris chloride BCC 0.08 M pH 8.5 Polyethylene glycol 4,000 24 w/v - Glycerol 20 v/v - 🔍	pH 8.5	200.0 μl of Glycerol (100 v/v) 80.0 μl of Magnesium chloride (2 M) 480.0 μl of Polyethylene glycol 4,000 (50 w/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 160.0 μl of water (100 v/v)
A7	Sodium cacodylate 0.07 M pH 6.5 Sodium acetate 0.98 M - Glycerol 30 v/v - 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 326.67 μl of Sodium acetate (3 M) 70.0 μl of Sodium cacodylate pH 6.5 (1 M) 303.33 μl of water (100 v/v)
A8	tri-Sodium citrate 0.14 M - Sodium cacodylate 0.07 M pH 6.5 2-Propanol 21 v/v - Glycerol 30 v/v - 🔍	pH 6.5	210.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium cacodylate pH 6.5 (1 M) 87.5 μl of tri-Sodium citrate (1.6 M) 332.5 μl of water (100 v/v)
A9	Ammonium acetate 0.17 M - tri-Sodium citrate 0.085 M pH 5.6 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 5.6	170.0 μl of Ammonium acetate (1 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of tri-Sodium citrate pH 5.6 (1 M) 85.0 μl of water (100 v/v)
A10	Ammonium acetate 0.17 M - Sodium acetate 0.085 M pH 4.6 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 4.6	170.0 μl of Ammonium acetate (1 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of Sodium acetate pH 4.6 (1 M) 85.0 μl of water (100 v/v)
A11	tri-Sodium citrate 0.07 M pH 5.6 Ammonium di-hydrogen phosphate 0.7 M - Glycerol 30 v/v - 🔍	pH 5.6	280.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of tri-Sodium citrate pH 5.6 (1 M) 350.0 μl of water (100 v/v)
A12	Magnesium chloride 0.18 M - HEPES 0.09 M pH 7.5 2-Propanol 27 v/v - Glycerol 10 v/v - 🔍	pH 7.5	270.0 μl of 2-Propanol (100 v/v) 100.0 μl of Glycerol (100 v/v) 90.0 μl of HEPES pH 7.5 (1 M) 90.0 μl of Magnesium chloride (2 M) 450.0 μl of water (100 v/v)
B1	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 30 v/v - 🔍	pH 8.5	300.0 μl of Polyethylene glycol 400 (100 v/v) 125.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 475.0 μl of water (100 v/v)
B2	Calcium chloride 0.19 M - HEPES 0.095 M pH 7.5 Polyethylene glycol 400 26.6 v/v - Glycerol 5 v/v - 🔍	pH 7.5	95.0 μl of Calcium chloride (2 M) 50.0 μl of Glycerol (100 v/v) 95.0 μl of HEPES pH 7.5 (1 M) 266.0 μl of Polyethylene glycol 400 (100 v/v) 494.0 μl of water (100 v/v)
B3	Ammonium sulfate 0.17 M - Sodium cacodylate 0.085 M pH 6.5 Polyethylene glycol 8,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 6.5	48.57 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 8,000 (50 w/v) 85.0 μl of Sodium cacodylate pH 6.5 (1 M) 206.43 μl of water (100 v/v)
B4	HEPES 0.075 M pH 7.5 Lithium sulfate 1.12 M - Glycerol 25 v/v - 🔍	pH 7.5	250.0 μl of Glycerol (100 v/v) 75.0 μl of HEPES pH 7.5 (1 M) 562.5 μl of Lithium sulfate (2 M) 112.5 μl of water (100 v/v)
B5	Lithium sulfate 0.17 M - tris chloride BCC 0.085 M pH 8.5 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 8.5	150.0 μl of Glycerol (100 v/v) 85.0 μl of Lithium sulfate (2 M) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 170.0 μl of water (100 v/v)
B6	Magnesium acetate 0.16 M - Sodium cacodylate 0.08 M pH 6.5 Polyethylene glycol 8,000 16 w/v - Glycerol 20 v/v - 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 160.0 μl of Magnesium acetate (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 240.0 μl of water (100 v/v)
B7	Ammonium acetate 0.16 M - tris chloride BCC 0.08 M pH 8.5 2-Propanol 24 v/v - Glycerol 20 v/v - 🔍	pH 8.5	240.0 μl of 2-Propanol (100 v/v) 160.0 μl of Ammonium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
B8	Ammonium sulfate 0.16 M - Sodium acetate 0.08 M pH 4.6 Polyethylene glycol 4,000 20 w/v - Glycerol 20 v/v - 🔍	pH 4.6	45.71 μl of Ammonium sulfate (3.5 M) 200.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 274.29 μl of water (100 v/v)
B9	Magnesium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 6.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Magnesium acetate (1 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v)
B10	Sodium acetate 0.17 M - tris chloride BCC 0.085 M pH 8.5 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 8.5	150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 56.67 μl of Sodium acetate (3 M) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 198.33 μl of water (100 v/v)
B11	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 30 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 500.0 μl of water (100 v/v)
B12	Calcium chloride 0.14 M - Sodium acetate 0.07 M pH 4.6 2-Propanol 14 v/v - Glycerol 30 v/v - 🔍	pH 4.6	140.0 μl of 2-Propanol (100 v/v) 70.0 μl of Calcium chloride (2 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 420.0 μl of water (100 v/v)
C1	Imidazole 0.07 M pH 6.5 Sodium acetate 0.7 M - Glycerol 30 v/v - 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 70.0 μl of Imidazole pH 6.5 (1 M) 233.33 μl of Sodium acetate (3 M) 396.67 μl of water (100 v/v)
C2	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 5.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v)
C3	tri-Sodium citrate 0.14 M - HEPES 0.07 M pH 7.5 2-Propanol 14 v/v - Glycerol 30 v/v - 🔍	pH 7.5	140.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 70.0 μl of HEPES pH 7.5 (1 M) 87.5 μl of tri-Sodium citrate (1.6 M) 402.5 μl of water (100 v/v)
C4	Sodium acetate 0.17 M - Sodium cacodylate 0.085 M pH 6.5 Polyethylene glycol 8,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 6.5	150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 8,000 (50 w/v) 56.67 μl of Sodium acetate (3 M) 85.0 μl of Sodium cacodylate pH 6.5 (1 M) 198.33 μl of water (100 v/v)
C5	HEPES 0.065 M pH 7.5 Potassium Sodium tartrate 0.52 M - Glycerol 35 v/v - 🔍	pH 7.5	350.0 μl of Glycerol (100 v/v) 65.0 μl of HEPES pH 7.5 (1 M) 346.67 μl of Potassium Sodium tartrate (1.5 M) 238.33 μl of water (100 v/v)
C6	Ammonium sulfate 0.17 M - Polyethylene glycol 8,000 25.5 w/v - Glycerol 15 v/v - 🔍	NA	48.57 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 8,000 (50 w/v) 291.43 μl of water (100 v/v)
C7	Ammonium sulfate 0.17 M - Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	NA	48.57 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 291.43 μl of water (100 v/v)
C8	Ammonium sulfate 1.5 M - Glycerol 25 v/v - 🔍	NA	428.57 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 321.43 μl of water (100 v/v)
C9	Sodium formate 3.6 M - Glycerol 10 v/v - 🔍	NA	100.0 μl of Glycerol (100 v/v) 514.29 μl of Sodium formate (7 M) 385.71 μl of water (100 v/v)
C10	Sodium formate 1.4 M - Sodium acetate 0.07 M pH 4.6 Glycerol 30 v/v - 🔍	pH 4.6	300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of Sodium formate (7 M) 430.0 μl of water (100 v/v)
C11	HEPES 0.075 M pH 7.5 Sodium di-hydrogen phosphate 0.6 M - Potassium di-hydrogen phosphate 0.6 M - Glycerol 25 v/v - 🔍	pH 7.5	250.0 μl of Glycerol (100 v/v) 75.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Potassium di-hydrogen phosphate (1.5 M) 150.0 μl of Sodium di-hydrogen phosphate (4 M) 125.0 μl of water (100 v/v)
C12	tris chloride BCC 0.065 M pH 8.5 Polyethylene glycol 8,000 5.2 w/v - Glycerol 35 v/v - 🔍	pH 8.5	350.0 μl of Glycerol (100 v/v) 104.0 μl of Polyethylene glycol 8,000 (50 w/v) 65.0 μl of tris chloride BCC pH 8.5 (1 M) 481.0 μl of water (100 v/v)
D1	Sodium acetate 0.07 M pH 4.6 Polyethylene glycol 4,000 5.6 w/v - Glycerol 30 v/v - 🔍	pH 4.6	300.0 μl of Glycerol (100 v/v) 112.0 μl of Polyethylene glycol 4,000 (50 w/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 518.0 μl of water (100 v/v)
D2	HEPES 0.09 M pH 7.5 tri-Sodium citrate 1.26 M - Glycerol 10 v/v - 🔍	pH 7.5	100.0 μl of Glycerol (100 v/v) 90.0 μl of HEPES pH 7.5 (1 M) 787.5 μl of tri-Sodium citrate (1.6 M) 22.5 μl of water (100 v/v)
D3	HEPES 0.085 M pH 7.5 Polyethylene glycol 400 1.7 v/v - Ammonium sulfate 1.7 M - Glycerol 15 v/v - 🔍	pH 7.5	485.71 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 17.0 μl of Polyethylene glycol 400 (100 v/v) 262.29 μl of water (100 v/v)
D4	tri-Sodium citrate 0.095 M pH 5.6 2-Propanol 19 v/v - Polyethylene glycol 4,000 19 w/v - Glycerol 5 v/v - 🔍	pH 5.6	190.0 μl of 2-Propanol (100 v/v) 50.0 μl of Glycerol (100 v/v) 380.0 μl of Polyethylene glycol 4,000 (50 w/v) 95.0 μl of tri-Sodium citrate pH 5.6 (1 M) 285.0 μl of water (100 v/v)
D5	HEPES 0.085 M pH 7.5 2-Propanol 8.5 v/v - Polyethylene glycol 4,000 17 w/v - Glycerol 15 v/v - 🔍	pH 7.5	85.0 μl of 2-Propanol (100 v/v) 150.0 μl of Glycerol (100 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 340.0 μl of Polyethylene glycol 4,000 (50 w/v) 340.0 μl of water (100 v/v)
D6	Potassium di-hydrogen phosphate 0.04 M - Polyethylene glycol 8,000 16 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 26.67 μl of Potassium di-hydrogen phosphate (1.5 M) 453.33 μl of water (100 v/v)
D7	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v)
D8	Magnesium formate 0.1 M - Glycerol 50 v/v - 🔍	NA	500.0 μl of Glycerol (100 v/v) 100.0 μl of Magnesium formate (1 M) 400.0 μl of water (100 v/v)
D9	Zinc acetate 0.16 M - Sodium cacodylate 0.08 M pH 6.5 Polyethylene glycol 8,000 14.4 w/v - Glycerol 20 v/v - 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 160.0 μl of Zinc acetate (1 M) 272.0 μl of water (100 v/v)
D10	Calcium acetate 0.16 M - Sodium cacodylate 0.08 M pH 6.5 Polyethylene glycol 8,000 14.4 w/v - Glycerol 20 v/v - 🔍	pH 6.5	160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 272.0 μl of water (100 v/v)
D11	Ammonium sulfate 1.6 M - Sodium acetate 0.08 M pH 4.6 Glycerol 20 v/v - 🔍	pH 4.6	457.14 μl of Ammonium sulfate (3.5 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 262.86 μl of water (100 v/v)
D12	tris chloride BCC 0.08 M pH 8.5 Ammonium di-hydrogen phosphate 1.6 M - Glycerol 20 v/v - 🔍	pH 8.5	640.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 80.0 μl of water (100 v/v)
E1	Lithium sulfate 0.8 M - Polyethylene glycol 8,000 1.6 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 400.0 μl of Lithium sulfate (2 M) 32.0 μl of Polyethylene glycol 8,000 (50 w/v) 368.0 μl of water (100 v/v)
E2	Lithium sulfate 0.4 M - Polyethylene glycol 8,000 12 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 200.0 μl of Lithium sulfate (2 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 360.0 μl of water (100 v/v)
E3		NA
E4		NA
E5		NA
E6		NA
E7		NA
E8		NA
E9		NA
E10		NA
E11		NA
E12		NA
F1		NA
F2		NA
F3		NA
F4		NA
F5		NA
F6		NA
F7		NA
F8		NA
F9		NA
F10		NA
F11		NA
F12		NA
G1		NA
G2		NA
G3		NA
G4		NA
G5		NA
G6		NA
G7		NA
G8		NA
G9		NA
G10		NA
G11		NA
G12		NA
H1		NA
H2		NA
H3		NA
H4		NA
H5		NA
H6		NA
H7		NA
H8		NA
H9		NA
H10		NA
H11		NA
H12		NA