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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
PS gradient
BCC Core Screens / PS gradient
Created 2025-03-06.
pH and salt gradient: The salt (0.5 - 3.0M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).
Export
PS gradient
Barcodes
PS gradient
- Z0002679
- Z0002714
- Z0002715
Screen Structure
PS gradient
Well Count | 96 |
---|---|
Number of distinct ingredients | 7 |
Internal diversity (score mean dist) | 0.779 |
Screen Composition
ingredient type | PS gradient |
---|---|
Buffer | 132 |
Salt | 108 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 500 μl of ammonium sulfate BCC (1 M) 500 μl of water (100 v/v) |
A2 |
| NA | 1000 μl of ammonium sulfate BCC (1 M) |
A3 |
| NA | 428 μl of ammonium sulfate BCC (3.5 M) 571 μl of water (100 v/v) |
A4 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 428 μl of water (100 v/v) |
A5 |
| NA | 714 μl of ammonium sulfate BCC (3.5 M) 285 μl of water (100 v/v) |
A6 |
| NA | 857 μl of ammonium sulfate BCC (3.5 M) 142 μl of water (100 v/v) |
A7 |
| NA | 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 852 μl of water (100 v/v) |
A8 |
| NA | 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 705 μl of water (100 v/v) |
A9 |
| NA | 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 558 μl of water (100 v/v) |
A10 |
| NA | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 411 μl of water (100 v/v) |
A11 |
| NA | 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 264 μl of water (100 v/v) |
A12 |
| NA | 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 117 μl of water (100 v/v) |
B1 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
B2 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
B3 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
B4 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
B5 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
B6 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
B7 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 752 μl of water (100 v/v) |
B8 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 605 μl of water (100 v/v) |
B9 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 458 μl of water (100 v/v) |
B10 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
B11 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 164 μl of water (100 v/v) |
B12 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 17 μl of water (100 v/v) |
C1 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
C2 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
C3 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
C4 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
C5 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
C6 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
C7 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 752 μl of water (100 v/v) |
C8 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 605 μl of water (100 v/v) |
C9 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 458 μl of water (100 v/v) |
C10 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
C11 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 164 μl of water (100 v/v) |
C12 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 17 μl of water (100 v/v) |
D1 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
D2 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
D3 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
D4 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
D5 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
D6 |
| pH 9 | 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
D7 |
| pH 9 | 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 752 μl of water (100 v/v) |
D8 |
| pH 9 | 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 605 μl of water (100 v/v) |
D9 |
| pH 9 | 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 458 μl of water (100 v/v) |
D10 |
| pH 9 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
D11 |
| pH 9 | 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 164 μl of water (100 v/v) |
D12 |
| pH 9 | 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 17 μl of water (100 v/v) |
E1 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
E2 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
E3 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
E4 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
E5 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
E6 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
E7 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 752 μl of water (100 v/v) |
E8 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 605 μl of water (100 v/v) |
E9 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 458 μl of water (100 v/v) |
E10 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 311 μl of water (100 v/v) |
E11 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 164 μl of water (100 v/v) |
E12 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 17 μl of water (100 v/v) |
F1 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
F2 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
F3 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
F4 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
F5 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
F6 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
F7 |
| pH 6.5 | 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 752 μl of water (100 v/v) |
F8 |
| pH 6.5 | 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 605 μl of water (100 v/v) |
F9 |
| pH 6.5 | 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 458 μl of water (100 v/v) |
F10 |
| pH 6.5 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 311 μl of water (100 v/v) |
F11 |
| pH 6.5 | 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 164 μl of water (100 v/v) |
F12 |
| pH 6.5 | 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 17 μl of water (100 v/v) |
G1 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
G2 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
G3 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
G4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
G5 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
G6 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
G7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 752 μl of water (100 v/v) |
G8 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 605 μl of water (100 v/v) |
G9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 458 μl of water (100 v/v) |
G10 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 311 μl of water (100 v/v) |
G11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 164 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 17 μl of water (100 v/v) |
H1 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 500 μl of ammonium sulfate BCC (1 M) 400 μl of water (100 v/v) |
H2 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 614 μl of water (100 v/v) |
H3 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 471 μl of water (100 v/v) |
H4 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
H5 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 714 μl of ammonium sulfate BCC (3.5 M) 185 μl of water (100 v/v) |
H6 |
| pH 8.5 | 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 857 μl of ammonium sulfate BCC (3.5 M) 42 μl of water (100 v/v) |
H7 |
| pH 8.5 | 147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 752 μl of water (100 v/v) |
H8 |
| pH 8.5 | 294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 605 μl of water (100 v/v) |
H9 |
| pH 8.5 | 441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 458 μl of water (100 v/v) |
H10 |
| pH 8.5 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 311 μl of water (100 v/v) |
H11 |
| pH 8.5 | 735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 164 μl of water (100 v/v) |
H12 |
| pH 8.5 | 882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 34 μl of gly-gly BCC pH 7.3 (1 M) 17 μl of water (100 v/v) |