C6 - Screen Contents - PS gradient

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Supplemental Information

Screen Description

PS gradient

BCC Core Screens / PS gradient

Created 2025-03-06.

pH and salt gradient: The salt (0.5 - 3.0M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

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PS gradient

Barcodes

PS gradient

Z0002714 Lot 2 (1500.0 µl)
Z0002715 Lot 3 (1500.0 µl)
Z0002679 Lot 1 (0.0 µl)

Screen Structure

PS gradient

Well Count	96
Number of distinct ingredients	7
Internal diversity (score mean dist)	0.779

Screen Composition

More information about screen composition chart.

ingredient type	PS gradient
Buffer	132
Salt	108

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

PS gradient Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	ammonium sulfate BCC 0.5 M - 🔍	NA	500.0 μl of ammonium sulfate BCC (1 M) 500.0 μl of water (100 v/v)
A2	ammonium sulfate BCC 1 M - 🔍	NA	1000.0 μl of ammonium sulfate BCC (1 M)
A3	ammonium sulfate BCC 1.5 M - 🔍	NA	428.6 μl of ammonium sulfate BCC (3.5 M) 571.4 μl of water (100 v/v)
A4	ammonium sulfate BCC 2 M - 🔍	NA	571.4 μl of ammonium sulfate BCC (3.5 M) 428.6 μl of water (100 v/v)
A5	ammonium sulfate BCC 2.5 M - 🔍	NA	714.3 μl of ammonium sulfate BCC (3.5 M) 285.7 μl of water (100 v/v)
A6	ammonium sulfate BCC 3 M - 🔍	NA	857.1 μl of ammonium sulfate BCC (3.5 M) 142.9 μl of water (100 v/v)
A7	sodium malonate-malonic acid BCC 0.5 M - 🔍	NA	147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 852.9 μl of water (100 v/v)
A8	sodium malonate-malonic acid BCC 1 M - 🔍	NA	294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 705.9 μl of water (100 v/v)
A9	sodium malonate-malonic acid BCC 1.5 M - 🔍	NA	441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 558.8 μl of water (100 v/v)
A10	sodium malonate-malonic acid BCC 2 M - 🔍	NA	588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 411.8 μl of water (100 v/v)
A11	sodium malonate-malonic acid BCC 2.5 M - 🔍	NA	735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 264.7 μl of water (100 v/v)
A12	sodium malonate-malonic acid BCC 3 M - 🔍	NA	882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 117.6 μl of water (100 v/v)
B1	ammonium sulfate BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of water (100 v/v)
B2	ammonium sulfate BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285.7 μl of ammonium sulfate BCC (3.5 M) 614.3 μl of water (100 v/v)
B3	ammonium sulfate BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428.6 μl of ammonium sulfate BCC (3.5 M) 471.4 μl of water (100 v/v)
B4	ammonium sulfate BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571.4 μl of ammonium sulfate BCC (3.5 M) 328.6 μl of water (100 v/v)
B5	ammonium sulfate BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714.3 μl of ammonium sulfate BCC (3.5 M) 185.7 μl of water (100 v/v)
B6	ammonium sulfate BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857.1 μl of ammonium sulfate BCC (3.5 M) 42.9 μl of water (100 v/v)
B7	sodium malonate-malonic acid BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
B8	sodium malonate-malonic acid BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
B9	sodium malonate-malonic acid BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
B10	sodium malonate-malonic acid BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
B11	sodium malonate-malonic acid BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
B12	sodium malonate-malonic acid BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 5 🔍	pH 5	80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)
C1	ammonium sulfate BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of water (100 v/v)
C2	ammonium sulfate BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285.7 μl of ammonium sulfate BCC (3.5 M) 614.3 μl of water (100 v/v)
C3	ammonium sulfate BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428.6 μl of ammonium sulfate BCC (3.5 M) 471.4 μl of water (100 v/v)
C4	ammonium sulfate BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571.4 μl of ammonium sulfate BCC (3.5 M) 328.6 μl of water (100 v/v)
C5	ammonium sulfate BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714.3 μl of ammonium sulfate BCC (3.5 M) 185.7 μl of water (100 v/v)
C6	ammonium sulfate BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857.1 μl of ammonium sulfate BCC (3.5 M) 42.9 μl of water (100 v/v)
C7	sodium malonate-malonic acid BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
C8	sodium malonate-malonic acid BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
C9	sodium malonate-malonic acid BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
C10	sodium malonate-malonic acid BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
C11	sodium malonate-malonic acid BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
C12	sodium malonate-malonic acid BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 7 🔍	pH 7	40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)
D1	ammonium sulfate BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500.0 μl of ammonium sulfate BCC (1 M) 400.0 μl of water (100 v/v)
D2	ammonium sulfate BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 285.7 μl of ammonium sulfate BCC (3.5 M) 614.3 μl of water (100 v/v)
D3	ammonium sulfate BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 428.6 μl of ammonium sulfate BCC (3.5 M) 471.4 μl of water (100 v/v)
D4	ammonium sulfate BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 571.4 μl of ammonium sulfate BCC (3.5 M) 328.6 μl of water (100 v/v)
D5	ammonium sulfate BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 714.3 μl of ammonium sulfate BCC (3.5 M) 185.7 μl of water (100 v/v)
D6	ammonium sulfate BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 857.1 μl of ammonium sulfate BCC (3.5 M) 42.9 μl of water (100 v/v)
D7	sodium malonate-malonic acid BCC 0.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
D8	sodium malonate-malonic acid BCC 1 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
D9	sodium malonate-malonic acid BCC 1.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
D10	sodium malonate-malonic acid BCC 2 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
D11	sodium malonate-malonic acid BCC 2.5 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
D12	sodium malonate-malonic acid BCC 3 M - DL-malate-MES-tris BCC 10 v/v pH 9 🔍	pH 9	100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)
E1	ammonium sulfate BCC 0.5 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	500.0 μl of ammonium sulfate BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400.0 μl of water (100 v/v)
E2	ammonium sulfate BCC 1 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	285.7 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 614.3 μl of water (100 v/v)
E3	ammonium sulfate BCC 1.5 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	428.6 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 471.4 μl of water (100 v/v)
E4	ammonium sulfate BCC 2 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	571.4 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 328.6 μl of water (100 v/v)
E5	ammonium sulfate BCC 2.5 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	714.3 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 185.7 μl of water (100 v/v)
E6	ammonium sulfate BCC 3 M - trisodium citrate-citric acid BCC 0.1 M pH 5.5 🔍	pH 5.5	857.1 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 42.9 μl of water (100 v/v)
E7	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 0.5 M - 🔍	pH 5.5	147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 752.9 μl of water (100 v/v)
E8	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 1 M - 🔍	pH 5.5	294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 605.9 μl of water (100 v/v)
E9	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 1.5 M - 🔍	pH 5.5	441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 458.8 μl of water (100 v/v)
E10	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 2 M - 🔍	pH 5.5	588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 311.8 μl of water (100 v/v)
E11	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 2.5 M - 🔍	pH 5.5	735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 164.7 μl of water (100 v/v)
E12	trisodium citrate-citric acid BCC 0.1 M pH 5.5 sodium malonate-malonic acid BCC 3 M - 🔍	pH 5.5	882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 17.6 μl of water (100 v/v)
F1	ammonium sulfate BCC 0.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	500.0 μl of ammonium sulfate BCC (1 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 400.0 μl of water (100 v/v)
F2	ammonium sulfate BCC 1 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	285.7 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 614.3 μl of water (100 v/v)
F3	ammonium sulfate BCC 1.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	428.6 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 471.4 μl of water (100 v/v)
F4	ammonium sulfate BCC 2 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 328.6 μl of water (100 v/v)
F5	ammonium sulfate BCC 2.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	714.3 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 185.7 μl of water (100 v/v)
F6	ammonium sulfate BCC 3 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	857.1 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 42.9 μl of water (100 v/v)
F7	sodium malonate-malonic acid BCC 0.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
F8	sodium malonate-malonic acid BCC 1 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
F9	sodium malonate-malonic acid BCC 1.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
F10	sodium malonate-malonic acid BCC 2 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
F11	sodium malonate-malonic acid BCC 2.5 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
F12	sodium malonate-malonic acid BCC 3 M - bis-tris chloride BCC 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)
G1	ammonium sulfate BCC 0.5 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	500.0 μl of ammonium sulfate BCC (1 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
G2	ammonium sulfate BCC 1 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	285.7 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 614.3 μl of water (100 v/v)
G3	ammonium sulfate BCC 1.5 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	428.6 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 471.4 μl of water (100 v/v)
G4	ammonium sulfate BCC 2 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	571.4 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v)
G5	ammonium sulfate BCC 2.5 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	714.3 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 185.7 μl of water (100 v/v)
G6	ammonium sulfate BCC 3 M - sodium HEPES BCC 0.1 M pH 7.5 🔍	pH 7.5	857.1 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 42.9 μl of water (100 v/v)
G7	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 0.5 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
G8	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 1 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
G9	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 1.5 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
G10	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 2 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
G11	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 2.5 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
G12	sodium HEPES BCC 0.1 M pH 7.5 sodium malonate-malonic acid BCC 3 M - 🔍	pH 7.5	50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)
H1	ammonium sulfate BCC 0.5 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	500.0 μl of ammonium sulfate BCC (1 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 400.0 μl of water (100 v/v)
H2	ammonium sulfate BCC 1 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	285.7 μl of ammonium sulfate BCC (3.5 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 614.3 μl of water (100 v/v)
H3	ammonium sulfate BCC 1.5 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	428.6 μl of ammonium sulfate BCC (3.5 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 471.4 μl of water (100 v/v)
H4	ammonium sulfate BCC 2 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	571.4 μl of ammonium sulfate BCC (3.5 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 328.6 μl of water (100 v/v)
H5	ammonium sulfate BCC 2.5 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	714.3 μl of ammonium sulfate BCC (3.5 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 185.7 μl of water (100 v/v)
H6	ammonium sulfate BCC 3 M - gly-gly BCC 0.1 M pH 8.5 🔍	pH 8.5	857.1 μl of ammonium sulfate BCC (3.5 M) 34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 42.9 μl of water (100 v/v)
H7	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 0.5 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 752.9 μl of water (100 v/v)
H8	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 1 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 605.9 μl of water (100 v/v)
H9	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 1.5 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 458.8 μl of water (100 v/v)
H10	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 2 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 311.8 μl of water (100 v/v)
H11	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 2.5 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 164.7 μl of water (100 v/v)
H12	gly-gly BCC 0.1 M pH 8.5 sodium malonate-malonic acid BCC 3 M - 🔍	pH 8.5	34.0 μl of gly-gly BCC pH 7.3 (1 M) 66.0 μl of gly-gly BCC pH 9.3 (1 M) 882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 17.6 μl of water (100 v/v)