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Supplemental Information

Screen Description

PS gradient

BCC Core Screens / PS gradient
Created 2025-03-06.
pH and salt gradient: The salt (0.5 - 3.0M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

Export

PS gradient

Barcodes

PS gradient

  • Z0002714 - 14/3/23 Lot 2 (1500.0 µl)
  • Z0002715 - 14/3/23 Lot 3 (1500.0 µl)
  • Z0002679 - 20/6/22 Lot 1 (0.0 µl)

Screen Structure

PS gradient

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.779

Screen Composition

More information about screen composition chart.
2025-08-11T20:36:03.988774 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PS gradient
Buffer 132
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PS gradient Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 0.5 M -
NA
500.0 μl of ammonium sulfate BCC (1 M)
500.0 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1 M -
NA
1000.0 μl of ammonium sulfate BCC (1 M)
A3
  • ammonium sulfate BCC 1.5 M -
NA
428.6 μl of ammonium sulfate BCC (3.5 M)
571.4 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 2 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
428.6 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2.5 M -
NA
714.3 μl of ammonium sulfate BCC (3.5 M)
285.7 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 3 M -
NA
857.1 μl of ammonium sulfate BCC (3.5 M)
142.9 μl of water (100 v/v)
A7
  • sodium malonate-malonic acid BCC 0.5 M -
NA
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
852.9 μl of water (100 v/v)
A8
  • sodium malonate-malonic acid BCC 1 M -
NA
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
705.9 μl of water (100 v/v)
A9
  • sodium malonate-malonic acid BCC 1.5 M -
NA
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
558.8 μl of water (100 v/v)
A10
  • sodium malonate-malonic acid BCC 2 M -
NA
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
411.8 μl of water (100 v/v)
A11
  • sodium malonate-malonic acid BCC 2.5 M -
NA
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
264.7 μl of water (100 v/v)
A12
  • sodium malonate-malonic acid BCC 3 M -
NA
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
117.6 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285.7 μl of ammonium sulfate BCC (3.5 M)
614.3 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428.6 μl of ammonium sulfate BCC (3.5 M)
471.4 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714.3 μl of ammonium sulfate BCC (3.5 M)
185.7 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857.1 μl of ammonium sulfate BCC (3.5 M)
42.9 μl of water (100 v/v)
B7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
B8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
B9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
B10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
B11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
B12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285.7 μl of ammonium sulfate BCC (3.5 M)
614.3 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428.6 μl of ammonium sulfate BCC (3.5 M)
471.4 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714.3 μl of ammonium sulfate BCC (3.5 M)
185.7 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857.1 μl of ammonium sulfate BCC (3.5 M)
42.9 μl of water (100 v/v)
C7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
C8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
C9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
C10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
C11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
C12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500.0 μl of ammonium sulfate BCC (1 M)
400.0 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285.7 μl of ammonium sulfate BCC (3.5 M)
614.3 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428.6 μl of ammonium sulfate BCC (3.5 M)
471.4 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714.3 μl of ammonium sulfate BCC (3.5 M)
185.7 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857.1 μl of ammonium sulfate BCC (3.5 M)
42.9 μl of water (100 v/v)
D7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
D8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
D9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
D10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
D11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 0.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
500.0 μl of ammonium sulfate BCC (1 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400.0 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
285.7 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
614.3 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 1.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
428.6 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
471.4 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
328.6 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 2.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
714.3 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
185.7 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
857.1 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
42.9 μl of water (100 v/v)
E7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 5.5
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
752.9 μl of water (100 v/v)
E8
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1 M -
pH 5.5
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
605.9 μl of water (100 v/v)
E9
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 5.5
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
458.8 μl of water (100 v/v)
E10
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2 M -
pH 5.5
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
311.8 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 5.5
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
164.7 μl of water (100 v/v)
E12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 3 M -
pH 5.5
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
17.6 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
500.0 μl of ammonium sulfate BCC (1 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
400.0 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
285.7 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
614.3 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 1.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
428.6 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
471.4 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
328.6 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 2.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
714.3 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
185.7 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
857.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
42.9 μl of water (100 v/v)
F7
  • sodium malonate-malonic acid BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
F8
  • sodium malonate-malonic acid BCC 1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
F9
  • sodium malonate-malonic acid BCC 1.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
F10
  • sodium malonate-malonic acid BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
F11
  • sodium malonate-malonic acid BCC 2.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
F12
  • sodium malonate-malonic acid BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 0.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of ammonium sulfate BCC (1 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
285.7 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
614.3 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
428.6 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 2.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
714.3 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
185.7 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
857.1 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
42.9 μl of water (100 v/v)
G7
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
G10
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
G11
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 3 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)
H1
  • ammonium sulfate BCC 0.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of ammonium sulfate BCC (1 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
400.0 μl of water (100 v/v)
H2
  • ammonium sulfate BCC 1 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
285.7 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
614.3 μl of water (100 v/v)
H3
  • ammonium sulfate BCC 1.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
471.4 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
328.6 μl of water (100 v/v)
H5
  • ammonium sulfate BCC 2.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
714.3 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
185.7 μl of water (100 v/v)
H6
  • ammonium sulfate BCC 3 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
857.1 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
42.9 μl of water (100 v/v)
H7
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
147.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
752.9 μl of water (100 v/v)
H8
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
294.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
605.9 μl of water (100 v/v)
H9
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
441.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
458.8 μl of water (100 v/v)
H10
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
H11
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
735.3 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
164.7 μl of water (100 v/v)
H12
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 3 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
882.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
17.6 μl of water (100 v/v)