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Supplemental Information

Screen Description

PS gradient

BCC Core Screens / PS gradient
Created 2025-03-06.
pH and salt gradient: The salt (0.5 - 3.0M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

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Barcodes

PS gradient

  • Z0002679
  • Z0002714
  • Z0002715

Screen Structure

PS gradient

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.779

Screen Composition

2025-06-12T23:00:26.404383 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type PS gradient
Buffer 132
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PS gradient Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 0.5 M -
NA
500 μl of ammonium sulfate BCC (1 M)
500 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1 M -
NA
1000 μl of ammonium sulfate BCC (1 M)
A3
  • ammonium sulfate BCC 1.5 M -
NA
428 μl of ammonium sulfate BCC (3.5 M)
571 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 2 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
428 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2.5 M -
NA
714 μl of ammonium sulfate BCC (3.5 M)
285 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 3 M -
NA
857 μl of ammonium sulfate BCC (3.5 M)
142 μl of water (100 v/v)
A7
  • sodium malonate-malonic acid BCC 0.5 M -
NA
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
852 μl of water (100 v/v)
A8
  • sodium malonate-malonic acid BCC 1 M -
NA
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
705 μl of water (100 v/v)
A9
  • sodium malonate-malonic acid BCC 1.5 M -
NA
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
558 μl of water (100 v/v)
A10
  • sodium malonate-malonic acid BCC 2 M -
NA
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
411 μl of water (100 v/v)
A11
  • sodium malonate-malonic acid BCC 2.5 M -
NA
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
264 μl of water (100 v/v)
A12
  • sodium malonate-malonic acid BCC 3 M -
NA
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
117 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
B7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
752 μl of water (100 v/v)
B8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
605 μl of water (100 v/v)
B9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
458 μl of water (100 v/v)
B10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
B11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
164 μl of water (100 v/v)
B12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
17 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
C7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
752 μl of water (100 v/v)
C8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
605 μl of water (100 v/v)
C9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
458 μl of water (100 v/v)
C10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
C11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
164 μl of water (100 v/v)
C12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
17 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
D7
  • sodium malonate-malonic acid BCC 0.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
752 μl of water (100 v/v)
D8
  • sodium malonate-malonic acid BCC 1 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
605 μl of water (100 v/v)
D9
  • sodium malonate-malonic acid BCC 1.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
458 μl of water (100 v/v)
D10
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
D11
  • sodium malonate-malonic acid BCC 2.5 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
164 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 3 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
17 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 0.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 1.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 2.5 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
E7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
752 μl of water (100 v/v)
E8
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
605 μl of water (100 v/v)
E9
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
458 μl of water (100 v/v)
E10
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
311 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
164 μl of water (100 v/v)
E12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 3 M -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
17 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 1.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 2.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
F7
  • sodium malonate-malonic acid BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
752 μl of water (100 v/v)
F8
  • sodium malonate-malonic acid BCC 1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
605 μl of water (100 v/v)
F9
  • sodium malonate-malonic acid BCC 1.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
458 μl of water (100 v/v)
F10
  • sodium malonate-malonic acid BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
311 μl of water (100 v/v)
F11
  • sodium malonate-malonic acid BCC 2.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
164 μl of water (100 v/v)
F12
  • sodium malonate-malonic acid BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
17 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 0.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 2.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
G7
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
752 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
605 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
458 μl of water (100 v/v)
G10
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
311 μl of water (100 v/v)
G11
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
164 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 3 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
17 μl of water (100 v/v)
H1
  • ammonium sulfate BCC 0.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
500 μl of ammonium sulfate BCC (1 M)
400 μl of water (100 v/v)
H2
  • ammonium sulfate BCC 1 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
614 μl of water (100 v/v)
H3
  • ammonium sulfate BCC 1.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
471 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
H5
  • ammonium sulfate BCC 2.5 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
714 μl of ammonium sulfate BCC (3.5 M)
185 μl of water (100 v/v)
H6
  • ammonium sulfate BCC 3 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
857 μl of ammonium sulfate BCC (3.5 M)
42 μl of water (100 v/v)
H7
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 0.5 M -
pH 8.5
147 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
752 μl of water (100 v/v)
H8
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1 M -
pH 8.5
294 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
605 μl of water (100 v/v)
H9
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.5 M -
pH 8.5
441 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
458 μl of water (100 v/v)
H10
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2 M -
pH 8.5
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
311 μl of water (100 v/v)
H11
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2.5 M -
pH 8.5
735 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
164 μl of water (100 v/v)
H12
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 3 M -
pH 8.5
882 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
34 μl of gly-gly BCC pH 7.3 (1 M)
17 μl of water (100 v/v)