Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Crystal Screen HT

Hampton Research / Crystal Screen HT
Created 2009-11-16.

Export

Barcodes

Crystal Screen HT

    Screen Structure

    Crystal Screen HT

    Well Count 96
    Number of distinct ingredients 56
    Internal diversity (score mean dist) 0.953

    Screen Composition

    2025-06-14T13:49:13.586766 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Crystal Screen HT
    Buffer 108
    Cryoprotectant 1
    Detergent 1
    Organic (non-volatile) 25
    Organic (volatile) 13
    Polymer 41
    Salt 116

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Crystal Screen HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    10 μl of Calcium chloride (2 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    590 μl of water (100 v/v)
    A2
    • Potassium Sodium tartrate 0.4 M -
    NA
    266 μl of Potassium Sodium tartrate (1.5 M)
    733 μl of water (100 v/v)
    A3
    • Ammonium di-hydrogen phosphate 0.4 M -
    NA
    160 μl of Ammonium di-hydrogen phosphate (2.5 M)
    840 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    475 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    100 μl of Magnesium chloride (2 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    A7
    • Sodium cacodylate 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    466 μl of Sodium acetate (3 M)
    433 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 30 v/v -
    pH 6.5
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    475 μl of water (100 v/v)
    A9
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of water (100 v/v)
    A10
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of water (100 v/v)
    A11
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 1 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of water (100 v/v)
    A12
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 30 v/v -
    pH 7.5
    100 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    500 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    475 μl of water (100 v/v)
    B2
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 v/v -
    pH 7.5
    100 μl of Calcium chloride (2 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    B3
    • Ammonium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    242 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    750 μl of Lithium sulfate (2 M)
    150 μl of water (100 v/v)
    B5
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    B6
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Magnesium acetate (1 M)
    300 μl of water (100 v/v)
    B7
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 30 v/v -
    pH 8.5
    200 μl of Ammonium acetate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    B8
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    342 μl of water (100 v/v)
    B9
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 6.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Magnesium acetate (1 M)
    400 μl of water (100 v/v)
    B10
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    B11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    B12
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 20 v/v -
    pH 4.6
    100 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    C1
    • Imidazole 0.1 M pH 6.5
    • Sodium acetate 1 M -
    pH 6.5
    333 μl of Sodium acetate (3 M)
    100 μl of Imidazole pH 6.5 (1 M)
    566 μl of water (100 v/v)
    C2
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 5.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 20 v/v -
    pH 7.5
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    575 μl of water (100 v/v)
    C4
    • Sodium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    66 μl of Sodium acetate (3 M)
    233 μl of water (100 v/v)
    C5
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.8 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Potassium Sodium tartrate (1.5 M)
    366 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 30 w/v -
    NA
    57 μl of Ammonium sulfate (3.5 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    342 μl of water (100 v/v)
    C7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    NA
    57 μl of Ammonium sulfate (3.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    342 μl of water (100 v/v)
    C8
    • Ammonium sulfate 2 M -
    NA
    571 μl of Ammonium sulfate (3.5 M)
    428 μl of water (100 v/v)
    C9
    • Sodium formate 4 M -
    NA
    571 μl of Sodium formate (7 M)
    428 μl of water (100 v/v)
    C10
    • Sodium formate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    285 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    C11
    • HEPES 0.1 M pH 7.5
    • Sodium di-hydrogen phosphate 0.8 M -
    • Potassium di-hydrogen phosphate 0.8 M -
    pH 7.5
    200 μl of Sodium di-hydrogen phosphate (4 M)
    533 μl of Potassium di-hydrogen phosphate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    166 μl of water (100 v/v)
    C12
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 8 w/v -
    pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    D1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    D2
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    pH 7.5
    875 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 v/v -
    • Ammonium sulfate 2 M -
    pH 7.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    308 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of water (100 v/v)
    D5
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    D6
    • Potassium di-hydrogen phosphate 0.05 M -
    • Polyethylene glycol 8,000 20 w/v -
    NA
    33 μl of Potassium di-hydrogen phosphate (1.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    566 μl of water (100 v/v)
    D7
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D8
    • Magnesium formate 0.2 M -
    NA
    200 μl of Magnesium formate (1 M)
    800 μl of water (100 v/v)
    D9
    • Zinc acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Zinc acetate (1 M)
    340 μl of water (100 v/v)
    D10
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Calcium acetate (1 M)
    340 μl of water (100 v/v)
    D11
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    328 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of water (100 v/v)
    E1
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    400 μl of water (100 v/v)
    E2
    • Sodium chloride 0.5 M -
    • Magnesium chloride 0.01 M -
    • Cetyltrimethylammonium bromide 10 mM -
    NA
    200 μl of Cetyltrimethylammonium bromide (50 mM)
    100 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    695 μl of water (100 v/v)
    E3
    • Ethylene glycol 25 v/v -
    NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    E4
    • 1,4-Dioxane 35 v/v -
    NA
    350 μl of 1,4-Dioxane (100 v/v)
    650 μl of water (100 v/v)
    E5
    • Ammonium sulfate 2 M -
    • 2-Propanol 5 v/v -
    NA
    571 μl of Ammonium sulfate (3.5 M)
    50 μl of 2-Propanol (100 v/v)
    378 μl of water (100 v/v)
    E6
    • Imidazole 1 M -
    NA
    1000 μl of Imidazole pH 7 (1 M)
    E7
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    600 μl of water (100 v/v)
    E8
    • Sodium chloride 1.5 M -
    • Ethanol 10 v/v -
    NA
    300 μl of Sodium chloride (5 M)
    100 μl of Ethanol (100 v/v)
    600 μl of water (100 v/v)
    E9
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    pH 4.6
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    E10
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    40 μl of Sodium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    560 μl of water (100 v/v)
    E11
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.1 M pH 4.6
    • 1,6-Hexanediol 1 M -
    pH 4.6
    10 μl of Cobalt (II) chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    166 μl of 1,6-Hexanediol (6 M)
    723 μl of water (100 v/v)
    E12
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.1 M -
    pH 4.6
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Cadmium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    F1
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 4.6
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    242 μl of water (100 v/v)
    F2
    • Potassium Sodium tartrate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    pH 5.6
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    133 μl of Potassium Sodium tartrate (1.5 M)
    195 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Lithium sulfate 1 M -
    pH 5.6
    142 μl of Ammonium sulfate (3.5 M)
    500 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    257 μl of water (100 v/v)
    F4
    • Sodium chloride 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ethylene imine polymer 2 v/v -
    pH 5.6
    100 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Ethylene imine polymer (50 v/v)
    760 μl of water (100 v/v)
    F5
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Methyl-2-propanol 35 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    875 μl of 2-Methyl-2-propanol (40 v/v)
    25 μl of water (100 v/v)
    F6
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    pH 5.6
    10 μl of Iron (III) chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Jeffamine® M-600 pH 7 (50 v/v)
    690 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.5 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    416 μl of 1,6-Hexanediol (6 M)
    483 μl of water (100 v/v)
    F8
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    pH 6.5
    800 μl of Magnesium sulfate (2 M)
    100 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    F9
    • Sodium di-hydrogen phosphate 0.1 M -
    • MES 0.1 M pH 6.5
    • Sodium chloride 2 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    25 μl of Sodium di-hydrogen phosphate (4 M)
    400 μl of Sodium chloride (5 M)
    66 μl of Potassium di-hydrogen phosphate (1.5 M)
    408 μl of water (100 v/v)
    F10
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    500 μl of water (100 v/v)
    F11
    • Ammonium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    • 1,4-Dioxane 10 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    100 μl of 1,4-Dioxane (100 v/v)
    342 μl of water (100 v/v)
    F12
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    250 μl of water (100 v/v)
    G1
    • Cobalt (II) chloride 0.01 M -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    10 μl of Cobalt (II) chloride (1 M)
    375 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    242 μl of water (100 v/v)
    G3
    • Zinc sulfate 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    5 μl of Zinc sulfate (2 M)
    645 μl of water (100 v/v)
    G4
    • tri-Sodium citrate 1.6 M -
    NA
    1000 μl of tri-Sodium citrate (1.6 M)
    G5
    • Ammonium sulfate 0.5 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    142 μl of Ammonium sulfate (3.5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    457 μl of water (100 v/v)
    G6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 10 w/v -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    pH 7.5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    G7
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Jeffamine® M-600 pH 7 (50 v/v)
    500 μl of water (100 v/v)
    G8
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    pH 7.5
    457 μl of Ammonium sulfate (3.5 M)
    20 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    422 μl of water (100 v/v)
    G9
    • HEPES 0.1 M pH 7.5
    • Ammonium formate 2 M -
    pH 7.5
    200 μl of Ammonium formate (10 M)
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    G10
    • Cadmium sulfate 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of Sodium acetate (3 M)
    50 μl of Cadmium sulfate (1 M)
    516 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    G12
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 4.3 M -
    pH 7.5
    860 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of water (100 v/v)
    H1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 8 v/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    H2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    H3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 M -
    pH 8.5
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    566 μl of 1,6-Hexanediol (6 M)
    233 μl of water (100 v/v)
    H4
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Methyl-2-propanol 25 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    625 μl of 2-Methyl-2-propanol (40 v/v)
    275 μl of water (100 v/v)
    H5
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    pH 8.5
    500 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    2 μl of Nickel (II) chloride (4 M)
    397 μl of water (100 v/v)
    H6
    • Ammonium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 12 v/v -
    pH 8.5
    428 μl of Ammonium sulfate (3.5 M)
    120 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    351 μl of water (100 v/v)
    H7
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    320 μl of water (100 v/v)
    H8
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    pH 8.5
    200 μl of Ethanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    H9
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    2 μl of Nickel (II) chloride (4 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    497 μl of water (100 v/v)
    H10
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 9
    20 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    680 μl of water (100 v/v)
    H11
    • BICINE 0.1 M pH 9
    • Magnesium chloride 2 M -
    pH 9
    400 μl of Magnesium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    H12
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    • Polyethylene glycol 20,000 10 w/v -
    pH 9
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    20 μl of 1,4-Dioxane (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    546 μl of water (100 v/v)