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Supplemental Information

Screen Description

Crystal Screen HT

Hampton Research / Crystal Screen HT
Created 2009-11-16.

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Crystal Screen HT

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Screen Structure

Crystal Screen HT

Well Count 96
Number of distinct ingredients 56
Internal diversity (score mean dist) 0.953

Screen Composition

2025-08-12T11:04:36.367297 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Crystal Screen HT
Buffer 108
Cryoprotectant 1
Detergent 1
Organic (non-volatile) 25
Organic (volatile) 13
Polymer 41
Salt 116

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Crystal Screen HT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
A2
  • Potassium Sodium tartrate 0.4 M -
NA
266.7 μl of Potassium Sodium tartrate (1.5 M)
733.3 μl of water (100 v/v)
A3
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
160.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
840.0 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A6
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A7
  • Sodium cacodylate 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
466.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
433.3 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 30 v/v -
pH 6.5
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A9
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
100.0 μl of water (100 v/v)
A10
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
A11
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
500.0 μl of water (100 v/v)
A12
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 30 v/v -
pH 7.5
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
B2
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
B3
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
242.9 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
B5
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
B6
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
B7
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 30 v/v -
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B8
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
342.9 μl of water (100 v/v)
B9
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Magnesium acetate (1 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B10
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
B12
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 20 v/v -
pH 4.6
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
600.0 μl of water (100 v/v)
C1
  • Imidazole 0.1 M pH 6.5
  • Sodium acetate 1 M -
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
333.3 μl of Sodium acetate (3 M)
566.7 μl of water (100 v/v)
C2
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
C3
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 20 v/v -
pH 7.5
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
575.0 μl of water (100 v/v)
C4
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
233.3 μl of water (100 v/v)
C5
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
533.3 μl of Potassium Sodium tartrate (1.5 M)
366.7 μl of water (100 v/v)
C6
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.9 μl of water (100 v/v)
C7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
342.9 μl of water (100 v/v)
C8
  • Ammonium sulfate 2 M -
NA
571.4 μl of Ammonium sulfate (3.5 M)
428.6 μl of water (100 v/v)
C9
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
C10
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
C11
  • HEPES 0.1 M pH 7.5
  • Sodium di-hydrogen phosphate 0.8 M -
  • Potassium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
533.3 μl of Potassium di-hydrogen phosphate (1.5 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
166.7 μl of water (100 v/v)
C12
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
D1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
  • Ammonium sulfate 2 M -
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
308.6 μl of water (100 v/v)
D4
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
D6
  • Potassium di-hydrogen phosphate 0.05 M -
  • Polyethylene glycol 8,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Potassium di-hydrogen phosphate (1.5 M)
566.7 μl of water (100 v/v)
D7
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
D8
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
800.0 μl of water (100 v/v)
D9
  • Zinc acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of Zinc acetate (1 M)
340.0 μl of water (100 v/v)
D10
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
340.0 μl of water (100 v/v)
D11
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
328.6 μl of water (100 v/v)
D12
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
E1
  • Sodium chloride 2 M -
  • Polyethylene glycol 6,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
E2
  • Sodium chloride 0.5 M -
  • Magnesium chloride 0.01 M -
  • Cetyltrimethylammonium bromide 10 mM -
NA
200.0 μl of Cetyltrimethylammonium bromide (50 mM)
5.0 μl of Magnesium chloride (2 M)
100.0 μl of Sodium chloride (5 M)
695.0 μl of water (100 v/v)
E3
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
E4
  • 1,4-Dioxane 35 v/v -
NA
350.0 μl of 1,4-Dioxane (100 v/v)
650.0 μl of water (100 v/v)
E5
  • Ammonium sulfate 2 M -
  • 2-Propanol 5 v/v -
NA
50.0 μl of 2-Propanol (100 v/v)
571.4 μl of Ammonium sulfate (3.5 M)
378.6 μl of water (100 v/v)
E6
  • Imidazole 1 M -
NA
1000.0 μl of Imidazole pH 7 (1 M)
E7
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
E8
  • Sodium chloride 1.5 M -
  • Ethanol 10 v/v -
NA
100.0 μl of Ethanol (100 v/v)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
E9
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
E10
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
E11
  • Cobalt (II) chloride 0.01 M -
  • Sodium acetate 0.1 M pH 4.6
  • 1,6-Hexanediol 1 M -
pH 4.6
166.7 μl of 1,6-Hexanediol (6 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
723.3 μl of water (100 v/v)
E12
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
  • Cadmium chloride 0.1 M -
pH 4.6
100.0 μl of Cadmium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
F1
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
242.9 μl of water (100 v/v)
F2
  • Potassium Sodium tartrate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
571.4 μl of Ammonium sulfate (3.5 M)
133.3 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
195.2 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.5 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 1 M -
pH 5.6
142.9 μl of Ammonium sulfate (3.5 M)
500.0 μl of Lithium sulfate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
257.1 μl of water (100 v/v)
F4
  • Sodium chloride 0.5 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ethylene imine polymer 2 v/v -
pH 5.6
40.0 μl of Ethylene imine polymer (50 v/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
760.0 μl of water (100 v/v)
F5
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Methyl-2-propanol 35 v/v -
pH 5.6
875.0 μl of 2-Methyl-2-propanol (40 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
25.0 μl of water (100 v/v)
F6
  • Iron (III) chloride 0.01 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Jeffamine® M-600 10 v/v -
pH 5.6
10.0 μl of Iron (III) chloride (1 M)
200.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
F7
  • tri-Sodium citrate 0.1 M pH 5.6
  • 1,6-Hexanediol 2.5 M -
pH 5.6
416.7 μl of 1,6-Hexanediol (6 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
483.3 μl of water (100 v/v)
F8
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
F9
  • Sodium di-hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
  • Potassium di-hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
66.7 μl of Potassium di-hydrogen phosphate (1.5 M)
400.0 μl of Sodium chloride (5 M)
25.0 μl of Sodium di-hydrogen phosphate (4 M)
408.3 μl of water (100 v/v)
F10
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
F11
  • Ammonium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
  • 1,4-Dioxane 10 v/v -
pH 6.5
100.0 μl of 1,4-Dioxane (100 v/v)
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
342.9 μl of water (100 v/v)
F12
  • Cesium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Jeffamine® M-600 30 v/v -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
G1
  • Cobalt (II) chloride 0.01 M -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
514.3 μl of Ammonium sulfate (3.5 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
375.7 μl of water (100 v/v)
G2
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
242.9 μl of water (100 v/v)
G3
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
5.0 μl of Zinc sulfate (2 M)
645.0 μl of water (100 v/v)
G4
  • tri-Sodium citrate 1.6 M -
NA
1000.0 μl of tri-Sodium citrate (1.6 M)
G5
  • Ammonium sulfate 0.5 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
457.1 μl of water (100 v/v)
G6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
G7
  • HEPES 0.1 M pH 7.5
  • Jeffamine® M-600 20 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
500.0 μl of water (100 v/v)
G8
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
pH 7.5
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Sodium chloride (5 M)
422.9 μl of water (100 v/v)
G9
  • HEPES 0.1 M pH 7.5
  • Ammonium formate 2 M -
pH 7.5
200.0 μl of Ammonium formate (10 M)
100.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of water (100 v/v)
G10
  • Cadmium sulfate 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1 M -
pH 7.5
50.0 μl of Cadmium sulfate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
333.3 μl of Sodium acetate (3 M)
516.7 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
G12
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)
H1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
H2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
H3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 1,6-Hexanediol 3.4 M -
pH 8.5
566.7 μl of 1,6-Hexanediol (6 M)
100.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
H4
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Methyl-2-propanol 25 v/v -
pH 8.5
625.0 μl of 2-Methyl-2-propanol (40 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
275.0 μl of water (100 v/v)
H5
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1 M -
pH 8.5
500.0 μl of Lithium sulfate (2 M)
2.5 μl of Nickel (II) chloride (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
397.5 μl of water (100 v/v)
H6
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 12 v/v -
pH 8.5
428.6 μl of Ammonium sulfate (3.5 M)
120.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
351.4 μl of water (100 v/v)
H7
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 20 v/v -
pH 8.5
200.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
H9
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
2.5 μl of Nickel (II) chloride (4 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
497.5 μl of water (100 v/v)
H10
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
H11
  • BICINE 0.1 M pH 9
  • Magnesium chloride 2 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Magnesium chloride (5 M)
500.0 μl of water (100 v/v)
H12
  • BICINE 0.1 M pH 9
  • 1,4-Dioxane 2 v/v -
  • Polyethylene glycol 20,000 10 w/v -
pH 9
20.0 μl of 1,4-Dioxane (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
333.3 μl of Polyethylene glycol 20,000 (30 w/v)
546.7 μl of water (100 v/v)