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Supplemental Information

Screen Description

Grid Screen Ammonium sulfate

Hampton Research / Grid Screen Ammonium sulfate
Created 2009-11-16.

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Grid Screen Ammonium sulfate

    Screen Structure

    Grid Screen Ammonium sulfate

    Well Count 24
    Number of distinct ingredients 6
    Internal diversity (score mean dist) 0.543

    Screen Composition

    2025-06-13T21:33:16.507973 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Grid Screen Ammonium sulfate
    Buffer 24
    Salt 32

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Grid Screen Ammonium sulfate Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 0.8 M -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A3
    • MES 0.1 M pH 6
    • Ammonium sulfate 0.8 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 0.8 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.8 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 0.8 M -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 1.6 M -
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 1.6 M -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6
    • Ammonium sulfate 1.6 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1.6 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1.6 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 1.6 M -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 2.4 M -
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 2.4 M -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 6
    • Ammonium sulfate 2.4 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2.4 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 2.4 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    C6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 3 M -
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 3 M -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6
    • Ammonium sulfate 3 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 3 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 3 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    D6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 3 M -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)