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Supplemental Information

Screen Description

Grid Screen PEG 6,000

Hampton Research / Grid Screen PEG 6,000
Created 2009-11-16.

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Grid Screen PEG 6,000

    Screen Structure

    Grid Screen PEG 6,000

    Well Count 24
    Number of distinct ingredients 6
    Internal diversity (score mean dist) 0.665

    Screen Composition

    2025-06-13T21:31:49.617089 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Grid Screen PEG 6,000
    Buffer 24
    Polymer 24
    Salt 8

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Grid Screen PEG 6,000 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 5 w/v -
    pH 4
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 5 w/v -
    pH 5
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    A3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 5 w/v -
    pH 6
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    800 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 5 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    800 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 5 w/v -
    pH 8
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 5 w/v -
    pH 9
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 10 w/v -
    pH 4
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    700 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    pH 5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 10 w/v -
    pH 6
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    700 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 10 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 10 w/v -
    pH 8
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    700 μl of water (100 v/v)
    B6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 10 w/v -
    pH 9
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    500 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    500 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    C6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 20 w/v -
    pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 30 w/v -
    pH 4
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    300 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 30 w/v -
    pH 5
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    300 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 30 w/v -
    pH 6
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    300 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 30 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 30 w/v -
    pH 8
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    D6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 30 w/v -
    pH 9
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)