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Supplemental Information

Screen Description

Grid Screen PEG 6,000

Hampton Research / Grid Screen PEG 6,000
Created 2009-11-16.

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Grid Screen PEG 6,000

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Screen Structure

Grid Screen PEG 6,000

Well Count 24
Number of distinct ingredients 6
Internal diversity (score mean dist) 0.665

Screen Composition

2025-08-12T11:04:36.667129 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Grid Screen PEG 6,000
Buffer 24
Polymer 24
Salt 8

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Grid Screen PEG 6,000 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 5 w/v -
pH 4
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
800.0 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 5 w/v -
pH 5
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
800.0 μl of water (100 v/v)
A3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 5 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
A4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 5 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
A5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 5 w/v -
pH 8
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
800.0 μl of water (100 v/v)
A6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 5 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 10 w/v -
pH 4
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
700.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
B3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 10 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 10 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
B5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 10 w/v -
pH 8
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
B6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 10 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
500.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
C3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 20 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 20 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 20 w/v -
pH 8
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
C6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 20 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 30 w/v -
pH 4
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
300.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 30 w/v -
pH 5
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
D3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 30 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 30 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 30 w/v -
pH 8
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 30 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)