C6 - Screen Contents - MembFac Screen

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Supplemental Information

Screen Description

MembFac Screen

Hampton Research / MembFac Screen

Created 2009-11-16.

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MembFac Screen

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MembFac Screen

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Screen Structure

MembFac Screen

Well Count	48
Number of distinct ingredients	23
Internal diversity (score mean dist)	0.912

Screen Composition

More information about screen composition chart.

ingredient type	MembFac Screen
Buffer	58
Organic (non-volatile)	15
Organic (volatile)	3
Polymer	21
Salt	75

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

MembFac Screen Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 4.6	120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 20.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v)
A2	Zinc acetate 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 4.6	240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of Zinc acetate (1 M) 560.0 μl of water (100 v/v)
A3	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 10 w/v - 🔍	pH 4.6	57.1 μl of Ammonium sulfate (3.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 642.9 μl of water (100 v/v)
A4	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 2-Propanol 12 v/v - 🔍	pH 4.6	120.0 μl of 2-Propanol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 20.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v)
A5	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 4.6	240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 660.0 μl of water (100 v/v)
A6	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 1 M - 🔍	pH 4.6	285.7 μl of Ammonium sulfate (3.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 614.3 μl of water (100 v/v)
A7	Sodium acetate 0.1 M pH 4.6 Magnesium sulfate 1 M - 🔍	pH 4.6	500.0 μl of Magnesium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of water (100 v/v)
A8	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 18 v/v - 🔍	pH 4.6	50.0 μl of Magnesium chloride (2 M) 180.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 670.0 μl of water (100 v/v)
B1	Lithium sulfate 0.1 M - Sodium acetate 0.1 M pH 4.6 Ammonium di-hydrogen phosphate 1 M - 🔍	pH 4.6	400.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 50.0 μl of Lithium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 450.0 μl of water (100 v/v)
B2	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 4.6	240.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 20.0 μl of Sodium chloride (5 M) 640.0 μl of water (100 v/v)
B3	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 4.6	50.0 μl of Magnesium chloride (2 M) 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 610.0 μl of water (100 v/v)
B4	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 400 18 v/v - 🔍	pH 5.6	180.0 μl of Polyethylene glycol 400 (100 v/v) 20.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 700.0 μl of water (100 v/v)
B5	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 5.6	50.0 μl of Lithium sulfate (2 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 610.0 μl of water (100 v/v)
B6	tri-Sodium citrate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - 🔍	pH 5.6	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 62.5 μl of tri-Sodium citrate (1.6 M) 737.5 μl of water (100 v/v)
B7	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 5.6	120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 760.0 μl of water (100 v/v)
B8	tri-Sodium citrate 0.1 M pH 5.6 Magnesium sulfate 1 M - 🔍	pH 5.6	500.0 μl of Magnesium sulfate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v)
C1	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 5.6	240.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 640.0 μl of water (100 v/v)
C2	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 5.6	50.0 μl of Lithium sulfate (2 M) 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 610.0 μl of water (100 v/v)
C3	Magnesium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 4 v/v - 🔍	pH 5.6	40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of Magnesium chloride (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 810.0 μl of water (100 v/v)
C4	tri-Sodium citrate 0.1 M pH 5.6 Sodium chloride 0.1 M - 🔍	pH 5.6	20.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 880.0 μl of water (100 v/v)
C5	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 400 4 v/v - 🔍	pH 5.6	50.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 810.0 μl of water (100 v/v)
C6	ADA 0.1 M pH 6.5 Ammonium sulfate 1 M - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 285.7 μl of Ammonium sulfate (3.5 M) 614.3 μl of water (100 v/v)
C7	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol 4,000 12 w/v - 2-Propanol 2 v/v - 🔍	pH 6.5	20.0 μl of 2-Propanol (100 v/v) 100.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Lithium sulfate (2 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 590.0 μl of water (100 v/v)
C8	ADA 0.1 M pH 6.5 di-Ammonium hydrogen phosphate 1 M - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 614.3 μl of water (100 v/v)
D1	Magnesium chloride 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol 6,000 12 w/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Magnesium chloride (2 M) 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 610.0 μl of water (100 v/v)
D2	ADA 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 6.5	120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of ADA pH 6.5 (1 M) 780.0 μl of water (100 v/v)
D3	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.5 Magnesium sulfate 1 M - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Lithium sulfate (2 M) 400.0 μl of Magnesium sulfate (2.5 M) 450.0 μl of water (100 v/v)
D4	Lithium sulfate 0.3 M - ADA 0.1 M pH 6.5 Polyethylene glycol 400 4 v/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 150.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 400 (100 v/v) 710.0 μl of water (100 v/v)
D5	Ammonium sulfate 0.1 M - HEPES 0.1 M pH 7.5 di-Sodium hydrogen phosphate 0.5 M - di-Potassium hydrogen phosphate 0.5 M - 🔍	pH 7.5	28.6 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 125.0 μl of di-Potassium hydrogen phosphate (4 M) 500.0 μl of di-Sodium hydrogen phosphate (1 M) 246.4 μl of water (100 v/v)
D6	Sodium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Sodium chloride (5 M) 680.0 μl of water (100 v/v)
D7	Magnesium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 18 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Magnesium chloride (2 M) 180.0 μl of Polyethylene glycol 400 (100 v/v) 670.0 μl of water (100 v/v)
D8	HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 1 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 666.7 μl of Potassium Sodium tartrate (1.5 M) 233.3 μl of water (100 v/v)
E1	Ammonium sulfate 0.1 M - Polyethylene glycol 400 18 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	28.6 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 180.0 μl of Polyethylene glycol 400 (100 v/v) 691.4 μl of water (100 v/v)
E2	Ammonium sulfate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	28.6 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 671.4 μl of water (100 v/v)
E3	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 7.5	120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 62.5 μl of tri-Sodium citrate (1.6 M) 717.5 μl of water (100 v/v)
E4	HEPES 0.1 M pH 7.5 tri-Sodium citrate 1 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v)
E5	Magnesium sulfate 0.6 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 4 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Magnesium sulfate (2.5 M) 40.0 μl of Polyethylene glycol 400 (100 v/v) 620.0 μl of water (100 v/v)
E6	Magnesium sulfate 0.6 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 4 v/v - 🔍	pH 7.5	40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Magnesium sulfate (2.5 M) 620.0 μl of water (100 v/v)
E7	Lithium sulfate 0.1 M - HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 0.1 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Lithium sulfate (2 M) 66.7 μl of Potassium Sodium tartrate (1.5 M) 783.3 μl of water (100 v/v)
E8	Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 8.5	120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of Lithium sulfate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 730.0 μl of water (100 v/v)
F1	di-Ammonium hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 di-Sodium hydrogen phosphate 0.5 M - di-Potassium hydrogen phosphate 0.5 M - 🔍	pH 8.5	28.6 μl of di-Ammonium hydrogen phosphate (3.5 M) 125.0 μl of di-Potassium hydrogen phosphate (4 M) 500.0 μl of di-Sodium hydrogen phosphate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 246.4 μl of water (100 v/v)
F2	tris chloride BCC 0.1 M pH 8.5 Sodium acetate 0.1 M - 🔍	pH 8.5	33.3 μl of Sodium acetate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 866.7 μl of water (100 v/v)
F3	tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.1 M - 🔍	pH 8.5	20.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 880.0 μl of water (100 v/v)
F4	di-Ammonium hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 6,000 12 w/v - 🔍	pH 8.5	240.0 μl of Polyethylene glycol 6,000 (50 w/v) 28.6 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 631.4 μl of water (100 v/v)
F5	Potassium Sodium tartrate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Magnesium sulfate 0.4 M - 🔍	pH 8.5	160.0 μl of Magnesium sulfate (2.5 M) 66.7 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 673.3 μl of water (100 v/v)
F6	tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	100.0 μl of Lithium sulfate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 800.0 μl of water (100 v/v)
F7	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 0.5 M - 🔍	pH 8.5	142.9 μl of Ammonium sulfate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 757.1 μl of water (100 v/v)
F8	tri-Sodium citrate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 5 v/v - 🔍	pH 8.5	50.0 μl of Polyethylene glycol 400 (100 v/v) 62.5 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 787.5 μl of water (100 v/v)