C6 - Screen Contents - MembFac Screen

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Supplemental Information

Screen Description

MembFac Screen

Hampton Research / MembFac Screen

Created 2009-11-16.

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MembFac Screen

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MembFac Screen

Screen Structure

MembFac Screen

Well Count	48
Number of distinct ingredients	23
Internal diversity (score mean dist)	0.912

Screen Composition

More information about screen composition chart.

ingredient type	MembFac Screen
Buffer	58
Organic (non-volatile)	15
Organic (volatile)	3
Polymer	21
Salt	75

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

MembFac Screen Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 4.6	120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Sodium chloride (5 M) 760 μl of water (100 v/v)
A2	Zinc acetate 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 4.6	100 μl of Zinc acetate (1 M) 100 μl of Sodium acetate pH 4.6 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 560 μl of water (100 v/v)
A3	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 10 w/v - 🔍	pH 4.6	57 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 642 μl of water (100 v/v)
A4	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 2-Propanol 12 v/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 120 μl of 2-Propanol (100 v/v) 20 μl of Sodium chloride (5 M) 760 μl of water (100 v/v)
A5	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 660 μl of water (100 v/v)
A6	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 1 M - 🔍	pH 4.6	285 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 614 μl of water (100 v/v)
A7	Sodium acetate 0.1 M pH 4.6 Magnesium sulfate 1 M - 🔍	pH 4.6	500 μl of Magnesium sulfate (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of water (100 v/v)
A8	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 18 v/v - 🔍	pH 4.6	50 μl of Magnesium chloride (2 M) 180 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Sodium acetate pH 4.6 (1 M) 670 μl of water (100 v/v)
B1	Lithium sulfate 0.1 M - Sodium acetate 0.1 M pH 4.6 Ammonium di-hydrogen phosphate 1 M - 🔍	pH 4.6	50 μl of Lithium sulfate (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 450 μl of water (100 v/v)
B2	Sodium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 4.6	240 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Sodium chloride (5 M) 640 μl of water (100 v/v)
B3	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 4.6	50 μl of Magnesium chloride (2 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 610 μl of water (100 v/v)
B4	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 400 18 v/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 180 μl of Polyethylene glycol 400 (100 v/v) 20 μl of Sodium chloride (5 M) 700 μl of water (100 v/v)
B5	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 50 μl of Lithium sulfate (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 610 μl of water (100 v/v)
B6	tri-Sodium citrate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 100 μl of 2-Propanol (100 v/v) 62 μl of tri-Sodium citrate (1.6 M) 737 μl of water (100 v/v)
B7	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20 μl of Sodium chloride (5 M) 760 μl of water (100 v/v)
B8	tri-Sodium citrate 0.1 M pH 5.6 Magnesium sulfate 1 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 500 μl of Magnesium sulfate (2 M) 400 μl of water (100 v/v)
C1	Sodium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 20 μl of Sodium chloride (5 M) 640 μl of water (100 v/v)
C2	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 6,000 12 w/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 50 μl of Lithium sulfate (2 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 610 μl of water (100 v/v)
C3	Magnesium chloride 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 4 v/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 50 μl of Magnesium chloride (2 M) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 810 μl of water (100 v/v)
C4	tri-Sodium citrate 0.1 M pH 5.6 Sodium chloride 0.1 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 20 μl of Sodium chloride (5 M) 880 μl of water (100 v/v)
C5	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 400 4 v/v - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 50 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 400 (100 v/v) 810 μl of water (100 v/v)
C6	ADA 0.1 M pH 6.5 Ammonium sulfate 1 M - 🔍	pH 6.5	285 μl of Ammonium sulfate (3.5 M) 100 μl of ADA pH 6.5 (1 M) 614 μl of water (100 v/v)
C7	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol 4,000 12 w/v - 2-Propanol 2 v/v - 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 50 μl of Lithium sulfate (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 20 μl of 2-Propanol (100 v/v) 590 μl of water (100 v/v)
C8	ADA 0.1 M pH 6.5 di-Ammonium hydrogen phosphate 1 M - 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 614 μl of water (100 v/v)
D1	Magnesium chloride 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol 6,000 12 w/v - 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 50 μl of Magnesium chloride (2 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 610 μl of water (100 v/v)
D2	ADA 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 780 μl of water (100 v/v)
D3	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.5 Magnesium sulfate 1 M - 🔍	pH 6.5	400 μl of Magnesium sulfate (2.5 M) 100 μl of ADA pH 6.5 (1 M) 50 μl of Lithium sulfate (2 M) 450 μl of water (100 v/v)
D4	Lithium sulfate 0.3 M - ADA 0.1 M pH 6.5 Polyethylene glycol 400 4 v/v - 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 150 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 400 (100 v/v) 710 μl of water (100 v/v)
D5	Ammonium sulfate 0.1 M - HEPES 0.1 M pH 7.5 di-Sodium hydrogen phosphate 0.5 M - di-Potassium hydrogen phosphate 0.5 M - 🔍	pH 7.5	28 μl of Ammonium sulfate (3.5 M) 100 μl of HEPES pH 7.5 (1 M) 500 μl of di-Sodium hydrogen phosphate (1 M) 125 μl of di-Potassium hydrogen phosphate (4 M) 246 μl of water (100 v/v)
D6	Sodium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 20 μl of Sodium chloride (5 M) 680 μl of water (100 v/v)
D7	Magnesium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 18 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 50 μl of Magnesium chloride (2 M) 180 μl of Polyethylene glycol 400 (100 v/v) 670 μl of water (100 v/v)
D8	HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 1 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 666 μl of Potassium Sodium tartrate (1.5 M) 233 μl of water (100 v/v)
E1	Ammonium sulfate 0.1 M - Polyethylene glycol 400 18 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	28 μl of Ammonium sulfate (3.5 M) 100 μl of HEPES pH 7.5 (1 M) 180 μl of Polyethylene glycol 400 (100 v/v) 691 μl of water (100 v/v)
E2	Ammonium sulfate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	28 μl of Ammonium sulfate (3.5 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 671 μl of water (100 v/v)
E3	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 62 μl of tri-Sodium citrate (1.6 M) 717 μl of water (100 v/v)
E4	HEPES 0.1 M pH 7.5 tri-Sodium citrate 1 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 625 μl of tri-Sodium citrate (1.6 M) 275 μl of water (100 v/v)
E5	Magnesium sulfate 0.6 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 4 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 240 μl of Magnesium sulfate (2.5 M) 40 μl of Polyethylene glycol 400 (100 v/v) 620 μl of water (100 v/v)
E6	Magnesium sulfate 0.6 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 4 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 240 μl of Magnesium sulfate (2.5 M) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 620 μl of water (100 v/v)
E7	Lithium sulfate 0.1 M - HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 0.1 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 50 μl of Lithium sulfate (2 M) 66 μl of Potassium Sodium tartrate (1.5 M) 783 μl of water (100 v/v)
E8	Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 12 v/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Lithium sulfate (2 M) 120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 730 μl of water (100 v/v)
F1	di-Ammonium hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 di-Sodium hydrogen phosphate 0.5 M - di-Potassium hydrogen phosphate 0.5 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of di-Sodium hydrogen phosphate (1 M) 28 μl of di-Ammonium hydrogen phosphate (3.5 M) 125 μl of di-Potassium hydrogen phosphate (4 M) 246 μl of water (100 v/v)
F2	tris chloride BCC 0.1 M pH 8.5 Sodium acetate 0.1 M - 🔍	pH 8.5	33 μl of Sodium acetate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 866 μl of water (100 v/v)
F3	tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.1 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 20 μl of Sodium chloride (5 M) 880 μl of water (100 v/v)
F4	di-Ammonium hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 6,000 12 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 28 μl of di-Ammonium hydrogen phosphate (3.5 M) 631 μl of water (100 v/v)
F5	Potassium Sodium tartrate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Magnesium sulfate 0.4 M - 🔍	pH 8.5	160 μl of Magnesium sulfate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Potassium Sodium tartrate (1.5 M) 673 μl of water (100 v/v)
F6	tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Lithium sulfate (2 M) 800 μl of water (100 v/v)
F7	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 0.5 M - 🔍	pH 8.5	142 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 757 μl of water (100 v/v)
F8	tri-Sodium citrate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 5 v/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Polyethylene glycol 400 (100 v/v) 62 μl of tri-Sodium citrate (1.6 M) 787 μl of water (100 v/v)