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Supplemental Information

Screen Description

MembFac Screen HT

Hampton Research / MembFac Screen HT
Created 2009-11-16.

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MembFac Screen HT

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Screen Structure

MembFac Screen HT

Well Count 96
Number of distinct ingredients 35
Internal diversity (score mean dist) 0.931

Screen Composition

2025-08-11T22:48:56.659967 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MembFac Screen HT
Buffer 120
Organic (non-volatile) 23
Organic (volatile) 10
Polymer 44
Salt 138

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MembFac Screen HT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 4.6
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
A2
  • Zinc acetate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of Zinc acetate (1 M)
560.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 10 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
642.9 μl of water (100 v/v)
A4
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 12 v/v -
pH 4.6
120.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
660.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 1 M -
pH 4.6
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
614.3 μl of water (100 v/v)
A7
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium sulfate 1 M -
pH 4.6
500.0 μl of Magnesium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
A8
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 18 v/v -
pH 4.6
50.0 μl of Magnesium chloride (2 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
670.0 μl of water (100 v/v)
A9
  • Lithium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 4.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
50.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
450.0 μl of water (100 v/v)
A10
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
A11
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
50.0 μl of Magnesium chloride (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
610.0 μl of water (100 v/v)
A12
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 18 v/v -
pH 5.6
180.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
700.0 μl of water (100 v/v)
B1
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
610.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
737.5 μl of water (100 v/v)
B3
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 5.6
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
760.0 μl of water (100 v/v)
B4
  • tri-Sodium citrate 0.1 M pH 5.6
  • Magnesium sulfate 1 M -
pH 5.6
500.0 μl of Magnesium sulfate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
B5
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
640.0 μl of water (100 v/v)
B6
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 6,000 12 w/v -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
610.0 μl of water (100 v/v)
B7
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
pH 5.6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Magnesium chloride (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
810.0 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.1 M -
pH 5.6
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
880.0 μl of water (100 v/v)
B9
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 4 v/v -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
810.0 μl of water (100 v/v)
B10
  • ADA 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
285.7 μl of Ammonium sulfate (3.5 M)
614.3 μl of water (100 v/v)
B11
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
  • 2-Propanol 2 v/v -
pH 6.5
20.0 μl of 2-Propanol (100 v/v)
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
590.0 μl of water (100 v/v)
B12
  • ADA 0.1 M pH 6.5
  • di-Ammonium hydrogen phosphate 1 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
614.3 μl of water (100 v/v)
C1
  • Magnesium chloride 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 6,000 12 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
610.0 μl of water (100 v/v)
C2
  • ADA 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 6.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of ADA pH 6.5 (1 M)
780.0 μl of water (100 v/v)
C3
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Magnesium sulfate 1 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
400.0 μl of Magnesium sulfate (2.5 M)
450.0 μl of water (100 v/v)
C4
  • Lithium sulfate 0.3 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 4 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
150.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
710.0 μl of water (100 v/v)
C5
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • di-Sodium hydrogen phosphate 0.5 M -
  • di-Potassium hydrogen phosphate 0.5 M -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of di-Potassium hydrogen phosphate (4 M)
500.0 μl of di-Sodium hydrogen phosphate (1 M)
246.4 μl of water (100 v/v)
C6
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
C7
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 18 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
670.0 μl of water (100 v/v)
C8
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
666.7 μl of Potassium Sodium tartrate (1.5 M)
233.3 μl of water (100 v/v)
C9
  • Ammonium sulfate 0.1 M -
  • Polyethylene glycol 400 18 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
691.4 μl of water (100 v/v)
C10
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
671.4 μl of water (100 v/v)
C11
  • tri-Sodium citrate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 7.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
717.5 μl of water (100 v/v)
C12
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
D1
  • Magnesium sulfate 0.6 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 4 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Magnesium sulfate (2.5 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
620.0 μl of water (100 v/v)
D2
  • Magnesium sulfate 0.6 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
pH 7.5
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Magnesium sulfate (2.5 M)
620.0 μl of water (100 v/v)
D3
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
66.7 μl of Potassium Sodium tartrate (1.5 M)
783.3 μl of water (100 v/v)
D4
  • Lithium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 8.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
730.0 μl of water (100 v/v)
D5
  • di-Ammonium hydrogen phosphate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • di-Sodium hydrogen phosphate 0.5 M -
  • di-Potassium hydrogen phosphate 0.5 M -
pH 8.5
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
125.0 μl of di-Potassium hydrogen phosphate (4 M)
500.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
246.4 μl of water (100 v/v)
D6
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.1 M -
pH 8.5
33.3 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
866.7 μl of water (100 v/v)
D7
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.1 M -
pH 8.5
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
880.0 μl of water (100 v/v)
D8
  • di-Ammonium hydrogen phosphate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
631.4 μl of water (100 v/v)
D9
  • Potassium Sodium tartrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium sulfate 0.4 M -
pH 8.5
160.0 μl of Magnesium sulfate (2.5 M)
66.7 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
673.3 μl of water (100 v/v)
D10
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 0.5 M -
pH 8.5
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
757.1 μl of water (100 v/v)
D12
  • tri-Sodium citrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 5 v/v -
pH 8.5
50.0 μl of Polyethylene glycol 400 (100 v/v)
62.5 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
787.5 μl of water (100 v/v)
E1
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
pH 4.6
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
E2
  • Potassium Sodium tartrate 0.2 M -
NA
133.3 μl of Potassium Sodium tartrate (1.5 M)
866.7 μl of water (100 v/v)
E3
  • Ammonium di-hydrogen phosphate 0.2 M -
NA
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
920.0 μl of water (100 v/v)
E4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1 M -
pH 8.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
614.3 μl of water (100 v/v)
E5
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
pH 7.5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
625.0 μl of water (100 v/v)
E6
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
E7
  • Sodium cacodylate 0.1 M pH 6.5
  • Sodium acetate 0.7 M -
pH 6.5
233.3 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
666.7 μl of water (100 v/v)
E8
  • tri-Sodium citrate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 15 v/v -
pH 6.5
150.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
625.0 μl of water (100 v/v)
E9
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 15 w/v -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
E10
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 15 w/v -
pH 4.6
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
E11
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 0.5 M -
pH 5.6
200.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
700.0 μl of water (100 v/v)
E12
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 15 v/v -
pH 7.5
150.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
650.0 μl of water (100 v/v)
F1
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 15 v/v -
pH 8.5
150.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
F2
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 14 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
140.0 μl of Polyethylene glycol 400 (100 v/v)
660.0 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 15 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
542.9 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.75 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
375.0 μl of Lithium sulfate (2 M)
525.0 μl of water (100 v/v)
F5
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
F6
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
200.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
F7
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 15 v/v -
pH 8.5
150.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
F8
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12.5 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
250.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
592.9 μl of water (100 v/v)
F9
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
pH 6.5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Magnesium acetate (1 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
F10
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
533.3 μl of water (100 v/v)
F11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 15 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
650.0 μl of water (100 v/v)
F12
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 10 v/v -
pH 4.6
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
G1
  • Imidazole 0.1 M pH 6.5
  • Sodium acetate 0.5 M -
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
166.7 μl of Sodium acetate (3 M)
733.3 μl of water (100 v/v)
G2
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
pH 5.6
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
550.0 μl of water (100 v/v)
G3
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
675.0 μl of water (100 v/v)
G4
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
533.3 μl of water (100 v/v)
G5
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
266.7 μl of Potassium Sodium tartrate (1.5 M)
633.3 μl of water (100 v/v)
G6
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
642.9 μl of water (100 v/v)
G7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 15 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
642.9 μl of water (100 v/v)
G8
  • Ammonium sulfate 1 M -
NA
285.7 μl of Ammonium sulfate (3.5 M)
714.3 μl of water (100 v/v)
G9
  • Sodium formate 2 M -
NA
285.7 μl of Sodium formate (7 M)
714.3 μl of water (100 v/v)
G10
  • Sodium formate 1 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
142.9 μl of Sodium formate (7 M)
757.1 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7.5
  • Sodium di-hydrogen phosphate 0.4 M -
  • Potassium di-hydrogen phosphate 0.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
266.7 μl of Potassium di-hydrogen phosphate (1.5 M)
100.0 μl of Sodium di-hydrogen phosphate (4 M)
533.3 μl of water (100 v/v)
G12
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 4 w/v -
pH 8.5
80.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
820.0 μl of water (100 v/v)
H1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 4 w/v -
pH 4.6
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
820.0 μl of water (100 v/v)
H2
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.7 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
437.5 μl of tri-Sodium citrate (1.6 M)
462.5 μl of water (100 v/v)
H3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
  • Ammonium sulfate 1 M -
pH 7.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
594.3 μl of water (100 v/v)
H4
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 10 w/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
600.0 μl of water (100 v/v)
H5
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 5 v/v -
  • Polyethylene glycol 4,000 10 w/v -
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
H6
  • Potassium di-hydrogen phosphate 0.05 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Potassium di-hydrogen phosphate (1.5 M)
766.7 μl of water (100 v/v)
H7
  • Polyethylene glycol 1,500 15 w/v -
NA
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
700.0 μl of water (100 v/v)
H8
  • Magnesium formate 0.1 M -
NA
100.0 μl of Magnesium formate (1 M)
900.0 μl of water (100 v/v)
H9
  • Zinc acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 9 w/v -
pH 6.5
180.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of Zinc acetate (1 M)
520.0 μl of water (100 v/v)
H10
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 9 w/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
180.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
520.0 μl of water (100 v/v)
H11
  • Ammonium sulfate 1 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
614.3 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 1 M -
pH 8.5
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)