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Supplemental Information

Screen Description

MembFac Screen HT

Hampton Research / MembFac Screen HT
Created 2009-11-16.

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MembFac Screen HT

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MembFac Screen HT

    Screen Structure

    MembFac Screen HT

    Well Count 96
    Number of distinct ingredients 35
    Internal diversity (score mean dist) 0.931

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:38:06.881652 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MembFac Screen HT
    Buffer 120
    Organic (non-volatile) 23
    Organic (volatile) 10
    Polymer 44
    Salt 138

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MembFac Screen HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    		pH 4.6
    20 μl of Sodium chloride (5 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    760 μl of water (100 v/v)
    A2
    • Zinc acetate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Zinc acetate (1 M)
    560 μl of water (100 v/v)
    A3
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 10 w/v -
    		pH 4.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    642 μl of water (100 v/v)
    A4
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 12 v/v -
    		pH 4.6
    120 μl of 2-Propanol (100 v/v)
    20 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    760 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    660 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    614 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1 M -
    		pH 4.6
    500 μl of Magnesium sulfate (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    A8
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 18 v/v -
    		pH 4.6
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Magnesium chloride (2 M)
    670 μl of water (100 v/v)
    A9
    • Lithium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium di-hydrogen phosphate 1 M -
    		pH 4.6
    50 μl of Lithium sulfate (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    450 μl of water (100 v/v)
    A10
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    		pH 4.6
    20 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    640 μl of water (100 v/v)
    A11
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Magnesium chloride (2 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    610 μl of water (100 v/v)
    A12
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 18 v/v -
    		pH 5.6
    180 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    700 μl of water (100 v/v)
    B1
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    610 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 10 v/v -
    		pH 5.6
    100 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    62 μl of tri-Sodium citrate (1.6 M)
    737 μl of water (100 v/v)
    B3
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    		pH 5.6
    20 μl of Sodium chloride (5 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    760 μl of water (100 v/v)
    B4
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium sulfate 1 M -
    		pH 5.6
    500 μl of Magnesium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of water (100 v/v)
    B5
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    640 μl of water (100 v/v)
    B6
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 6,000 12 w/v -
    		pH 5.6
    50 μl of Lithium sulfate (2 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    610 μl of water (100 v/v)
    B7
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    		pH 5.6
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    810 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.1 M -
    		pH 5.6
    20 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    880 μl of water (100 v/v)
    B9
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 4 v/v -
    		pH 5.6
    40 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    810 μl of water (100 v/v)
    B10
    • ADA 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    		pH 6.5
    100 μl of ADA pH 6.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    614 μl of water (100 v/v)
    B11
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    • 2-Propanol 2 v/v -
    		pH 6.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Lithium sulfate (2 M)
    20 μl of 2-Propanol (100 v/v)
    100 μl of ADA pH 6.5 (1 M)
    590 μl of water (100 v/v)
    B12
    • ADA 0.1 M pH 6.5
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 6.5
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of ADA pH 6.5 (1 M)
    614 μl of water (100 v/v)
    C1
    • Magnesium chloride 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 6,000 12 w/v -
    		pH 6.5
    50 μl of Magnesium chloride (2 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of ADA pH 6.5 (1 M)
    610 μl of water (100 v/v)
    C2
    • ADA 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    		pH 6.5
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of ADA pH 6.5 (1 M)
    780 μl of water (100 v/v)
    C3
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Magnesium sulfate 1 M -
    		pH 6.5
    50 μl of Lithium sulfate (2 M)
    400 μl of Magnesium sulfate (2.5 M)
    100 μl of ADA pH 6.5 (1 M)
    450 μl of water (100 v/v)
    C4
    • Lithium sulfate 0.3 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 4 v/v -
    		pH 6.5
    40 μl of Polyethylene glycol 400 (100 v/v)
    150 μl of Lithium sulfate (2 M)
    100 μl of ADA pH 6.5 (1 M)
    710 μl of water (100 v/v)
    C5
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • di-Sodium hydrogen phosphate 0.5 M -
    • di-Potassium hydrogen phosphate 0.5 M -
    		pH 7.5
    125 μl of di-Potassium hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of di-Sodium hydrogen phosphate (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    246 μl of water (100 v/v)
    C6
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    C7
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 18 v/v -
    		pH 7.5
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    670 μl of water (100 v/v)
    C8
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    666 μl of Potassium Sodium tartrate (1.5 M)
    233 μl of water (100 v/v)
    C9
    • Ammonium sulfate 0.1 M -
    • Polyethylene glycol 400 18 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    691 μl of water (100 v/v)
    C10
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    C11
    • tri-Sodium citrate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    717 μl of water (100 v/v)
    C12
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    D1
    • Magnesium sulfate 0.6 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 4 v/v -
    		pH 7.5
    40 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Magnesium sulfate (2.5 M)
    620 μl of water (100 v/v)
    D2
    • Magnesium sulfate 0.6 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    240 μl of Magnesium sulfate (2.5 M)
    620 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Lithium sulfate (2 M)
    66 μl of Potassium Sodium tartrate (1.5 M)
    783 μl of water (100 v/v)
    D4
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    		pH 8.5
    50 μl of Lithium sulfate (2 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    730 μl of water (100 v/v)
    D5
    • di-Ammonium hydrogen phosphate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium hydrogen phosphate 0.5 M -
    • di-Potassium hydrogen phosphate 0.5 M -
    		pH 8.5
    125 μl of di-Potassium hydrogen phosphate (4 M)
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    500 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    246 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 0.1 M -
    		pH 8.5
    33 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    866 μl of water (100 v/v)
    D7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    		pH 8.5
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    880 μl of water (100 v/v)
    D8
    • di-Ammonium hydrogen phosphate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 12 w/v -
    		pH 8.5
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    631 μl of water (100 v/v)
    D9
    • Potassium Sodium tartrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium sulfate 0.4 M -
    		pH 8.5
    66 μl of Potassium Sodium tartrate (1.5 M)
    160 μl of Magnesium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    673 μl of water (100 v/v)
    D10
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.5 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    142 μl of Ammonium sulfate (3.5 M)
    757 μl of water (100 v/v)
    D12
    • tri-Sodium citrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 5 v/v -
    		pH 8.5
    50 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    62 μl of tri-Sodium citrate (1.6 M)
    787 μl of water (100 v/v)
    E1
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    		pH 4.6
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    10 μl of Calcium chloride (2 M)
    740 μl of water (100 v/v)
    E2
    • Potassium Sodium tartrate 0.2 M -
    		NA
    133 μl of Potassium Sodium tartrate (1.5 M)
    866 μl of water (100 v/v)
    E3
    • Ammonium di-hydrogen phosphate 0.2 M -
    		NA
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    920 μl of water (100 v/v)
    E4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    614 μl of water (100 v/v)
    E5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    625 μl of water (100 v/v)
    E6
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    E7
    • Sodium cacodylate 0.1 M pH 6.5
    • Sodium acetate 0.7 M -
    		pH 6.5
    233 μl of Sodium acetate (3 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    666 μl of water (100 v/v)
    E8
    • tri-Sodium citrate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 15 v/v -
    		pH 6.5
    150 μl of 2-Propanol (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    625 μl of water (100 v/v)
    E9
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 15 w/v -
    		pH 5.6
    200 μl of Ammonium acetate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of water (100 v/v)
    E10
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 15 w/v -
    		pH 4.6
    200 μl of Ammonium acetate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    E11
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 0.5 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Ammonium di-hydrogen phosphate (2.5 M)
    700 μl of water (100 v/v)
    E12
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 15 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of 2-Propanol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    650 μl of water (100 v/v)
    F1
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 15 v/v -
    		pH 8.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    625 μl of water (100 v/v)
    F2
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 14 v/v -
    		pH 7.5
    140 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium chloride (2 M)
    660 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 15 w/v -
    		pH 6.5
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    542 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 0.75 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    375 μl of Lithium sulfate (2 M)
    525 μl of water (100 v/v)
    F5
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    F6
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 6.5
    200 μl of Magnesium acetate (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of water (100 v/v)
    F7
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 15 v/v -
    		pH 8.5
    200 μl of Ammonium acetate (1 M)
    150 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    550 μl of water (100 v/v)
    F8
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12.5 w/v -
    		pH 4.6
    250 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    592 μl of water (100 v/v)
    F9
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    		pH 6.5
    200 μl of Magnesium acetate (1 M)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    550 μl of water (100 v/v)
    F10
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    533 μl of water (100 v/v)
    F11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 15 v/v -
    		pH 7.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    650 μl of water (100 v/v)
    F12
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 10 v/v -
    		pH 4.6
    100 μl of 2-Propanol (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Calcium chloride (2 M)
    700 μl of water (100 v/v)
    G1
    • Imidazole 0.1 M pH 6.5
    • Sodium acetate 0.5 M -
    		pH 6.5
    166 μl of Sodium acetate (3 M)
    100 μl of Imidazole pH 6.5 (1 M)
    733 μl of water (100 v/v)
    G2
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    		pH 5.6
    200 μl of Ammonium acetate (1 M)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    550 μl of water (100 v/v)
    G3
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    675 μl of water (100 v/v)
    G4
    • Sodium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 15 w/v -
    		pH 6.5
    66 μl of Sodium acetate (3 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    533 μl of water (100 v/v)
    G5
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.4 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    266 μl of Potassium Sodium tartrate (1.5 M)
    633 μl of water (100 v/v)
    G6
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 15 w/v -
    		NA
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    642 μl of water (100 v/v)
    G7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 15 w/v -
    		NA
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    642 μl of water (100 v/v)
    G8
    • Ammonium sulfate 1 M -
    		NA
    285 μl of Ammonium sulfate (3.5 M)
    714 μl of water (100 v/v)
    G9
    • Sodium formate 2 M -
    		NA
    285 μl of Sodium formate (7 M)
    714 μl of water (100 v/v)
    G10
    • Sodium formate 1 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    142 μl of Sodium formate (7 M)
    757 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • Sodium di-hydrogen phosphate 0.4 M -
    • Potassium di-hydrogen phosphate 0.4 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Sodium di-hydrogen phosphate (4 M)
    266 μl of Potassium di-hydrogen phosphate (1.5 M)
    533 μl of water (100 v/v)
    G12
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 4 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Polyethylene glycol 8,000 (50 w/v)
    820 μl of water (100 v/v)
    H1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 4 w/v -
    		pH 4.6
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    820 μl of water (100 v/v)
    H2
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.7 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    437 μl of tri-Sodium citrate (1.6 M)
    462 μl of water (100 v/v)
    H3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 v/v -
    • Ammonium sulfate 1 M -
    		pH 7.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    594 μl of water (100 v/v)
    H4
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 10 w/v -
    		pH 5.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    600 μl of water (100 v/v)
    H5
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 5 v/v -
    • Polyethylene glycol 4,000 10 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of 2-Propanol (100 v/v)
    650 μl of water (100 v/v)
    H6
    • Potassium di-hydrogen phosphate 0.05 M -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    33 μl of Potassium di-hydrogen phosphate (1.5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    766 μl of water (100 v/v)
    H7
    • Polyethylene glycol 1,500 15 w/v -
    		NA
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    700 μl of water (100 v/v)
    H8
    • Magnesium formate 0.1 M -
    		NA
    100 μl of Magnesium formate (1 M)
    900 μl of water (100 v/v)
    H9
    • Zinc acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 9 w/v -
    		pH 6.5
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Zinc acetate (1 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    520 μl of water (100 v/v)
    H10
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 9 w/v -
    		pH 6.5
    200 μl of Calcium acetate (1 M)
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    520 μl of water (100 v/v)
    H11
    • Ammonium sulfate 1 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    614 μl of water (100 v/v)
    H12
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 1 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of water (100 v/v)