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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG Ion Screen 2
Barcodes
PEG Ion Screen 2
No barcodes found.
Screen Structure
PEG Ion Screen 2
Well Count | 48 |
---|---|
Number of distinct ingredients | 24 |
Internal diversity (score mean dist) | 0.659 |
Screen Composition
ingredient type | PEG Ion Screen 2 |
---|---|
Buffer | 48 |
Complex ingredient | 15 |
Polymer | 48 |
Salt | 45 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 29.4 μl of di-Sodium malonate pH 4 (3.4 M) 730.6 μl of water (100 v/v) |
A2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.8 μl of di-Sodium malonate pH 4 (3.4 M) 541.2 μl of water (100 v/v) |
A3 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 29.4 μl of di-Sodium malonate pH 5 (3.4 M) 730.6 μl of water (100 v/v) |
A4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.8 μl of di-Sodium malonate pH 5 (3.4 M) 541.2 μl of water (100 v/v) |
A5 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 29.4 μl of di-Sodium malonate pH 6 (3.4 M) 730.6 μl of water (100 v/v) |
A6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.8 μl of di-Sodium malonate pH 6 (3.4 M) 541.2 μl of water (100 v/v) |
A7 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 29.4 μl of di-Sodium malonate pH 7 (3.4 M) 730.6 μl of water (100 v/v) |
A8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.8 μl of di-Sodium malonate pH 7 (3.4 M) 541.2 μl of water (100 v/v) |
B1 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Tacsimate pH 7 (100 v/v) 720.0 μl of water (100 v/v) |
B2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of Tacsimate pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
B3 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Tacsimate pH 7 (100 v/v) 720.0 μl of water (100 v/v) |
B4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of Tacsimate pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
B5 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Tacsimate pH 7 (100 v/v) 720.0 μl of water (100 v/v) |
B6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of Tacsimate pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
B7 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 120.0 μl of Tacsimate pH 7 (100 v/v) 640.0 μl of water (100 v/v) |
B8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of Tacsimate pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
C1 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Tacsimate pH 7 (100 v/v) 720.0 μl of water (100 v/v) |
C2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of Tacsimate pH 7 (100 v/v) 520.0 μl of water (100 v/v) |
C3 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 83.3 μl of di-Sodium succinate (1.2 M) 676.7 μl of water (100 v/v) |
C4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 166.7 μl of di-Sodium succinate (1.2 M) 433.3 μl of water (100 v/v) |
C5 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of tri-Ammonium citrate (2.5 M) 720.0 μl of water (100 v/v) |
C6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Ammonium citrate (2.5 M) 520.0 μl of water (100 v/v) |
C7 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of di-Sodium DL-malate (3 M) 726.7 μl of water (100 v/v) |
C8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium DL-malate (3 M) 533.3 μl of water (100 v/v) |
D1 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium acetate (4 M) 735.0 μl of water (100 v/v) |
D2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Sodium acetate (4 M) 550.0 μl of water (100 v/v) |
D3 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Sodium formate (5 M) 740.0 μl of water (100 v/v) |
D4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium formate (5 M) 560.0 μl of water (100 v/v) |
D5 |
| NA | 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 62.5 μl of di-Ammonium tartrate (1.6 M) 697.5 μl of water (100 v/v) |
D6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 125.0 μl of di-Ammonium tartrate (1.6 M) 475.0 μl of water (100 v/v) |
D7 |
| pH 4.6 | 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 20.0 μl of Tacsimate pH 7 (100 v/v) 560.0 μl of water (100 v/v) |
D8 |
| pH 5.6 | 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Tacsimate pH 7 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 560.0 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Tacsimate pH 7 (100 v/v) 480.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Tacsimate pH 7 (100 v/v) 480.0 μl of water (100 v/v) |
E3 |
| pH 8.5 | 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Tacsimate pH 7 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 560.0 μl of water (100 v/v) |
E4 |
| pH 3.4 | 100.0 μl of CBTP buffer pH 2.2 (1 M) 0.0 μl of CBTP buffer pH 7.2 (1 M) 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
E5 |
| pH 4.1 | 99.8 μl of CBTP buffer pH 2.2 (1 M) 0.2 μl of CBTP buffer pH 7.2 (1 M) 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
E6 |
| pH 5 | 0.0 μl of CBTP buffer pH 2.2 (1 M) 0.0 μl of CBTP buffer pH 7.2 (1 M) 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 680.0 μl of water (100 v/v) |
E7 |
| pH 6.4 | 0.0 μl of CBTP buffer pH 2.2 (1 M) 0.0 μl of CBTP buffer pH 7.2 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 600.0 μl of water (100 v/v) |
E8 |
| pH 7.6 | 81.2 μl of CBTP buffer pH 8.8 (1 M) 18.8 μl of CBTP buffer pH 2.2 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F1 |
| pH 8.8 | 100.0 μl of CBTP buffer pH 8.8 (1 M) -0.0 μl of CBTP buffer pH 2.2 (1 M) 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
F2 |
| NA | 20.0 μl of Cadmium chloride (1 M) 10.0 μl of Calcium chloride (2 M) 20.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 550.0 μl of water (100 v/v) |
F3 |
| NA | 5.0 μl of Magnesium chloride (2 M) 50.0 μl of Nickel (II) chloride (0.1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 545.0 μl of water (100 v/v) |
F4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 10.0 μl of Zinc chloride (2 M) 590.0 μl of water (100 v/v) |
F5 |
| NA | 150.0 μl of Cesium chloride (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 550.0 μl of water (100 v/v) |
F6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium bromide (5 M) 560.0 μl of water (100 v/v) |
F7 |
| pH 7 | 50.0 μl of HEPES pH 7 (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Tryptone (10 w/v) 610.0 μl of water (100 v/v) |
F8 |
| pH 7 | 50.0 μl of HEPES pH 7 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Tryptone (10 w/v) 450.0 μl of water (100 v/v) |