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Supplemental Information

Screen Description

PEG Ion Screen 2

Hampton Research / PEG Ion Screen 2
Created 2009-11-16.

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PEG Ion Screen 2

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PEG Ion Screen 2

    Screen Structure

    PEG Ion Screen 2

    Well Count 48
    Number of distinct ingredients 24
    Internal diversity (score mean dist) 0.659

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:33:16.678996 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Ion Screen 2
    Buffer 48
    Complex ingredient 15
    Polymer 48
    Salt 45

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Ion Screen 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 4 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    A2
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 4 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    A3
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    29 μl of di-Sodium malonate pH 5 (3.4 M)
    730 μl of water (100 v/v)
    A4
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of di-Sodium malonate pH 5 (3.4 M)
    541 μl of water (100 v/v)
    A5
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 6 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    A6
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 6 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    A7
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 7 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    A8
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    B1
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    B2
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    B3
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    B4
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    B5
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    B6
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    B7
    • Tacsimate 12 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    120 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    640 μl of water (100 v/v)
    B8
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    C1
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    C2
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    C3
    • di-Sodium succinate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    83 μl of di-Sodium succinate (1.2 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    676 μl of water (100 v/v)
    C4
    • di-Sodium succinate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    166 μl of di-Sodium succinate (1.2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    433 μl of water (100 v/v)
    C5
    • tri-Ammonium citrate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of tri-Ammonium citrate (2.5 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    C6
    • tri-Ammonium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of tri-Ammonium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    C7
    • di-Sodium DL-malate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    33 μl of di-Sodium DL-malate (3 M)
    726 μl of water (100 v/v)
    C8
    • di-Sodium DL-malate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of di-Sodium DL-malate (3 M)
    533 μl of water (100 v/v)
    D1
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    25 μl of Sodium acetate (4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    735 μl of water (100 v/v)
    D2
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    50 μl of Sodium acetate (4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    D3
    • Sodium formate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Sodium formate (5 M)
    740 μl of water (100 v/v)
    D4
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium formate (5 M)
    560 μl of water (100 v/v)
    D5
    • di-Ammonium tartrate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    62 μl of di-Ammonium tartrate (1.6 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    697 μl of water (100 v/v)
    D6
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    125 μl of di-Ammonium tartrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    D7
    • Tacsimate 2 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 3,350 16 w/v -
    		pH 4.6
    20 μl of Tacsimate pH 7 (100 v/v)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    560 μl of water (100 v/v)
    D8
    • Tacsimate 2 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 3,350 16 w/v -
    		pH 5.6
    20 μl of Tacsimate pH 7 (100 v/v)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    560 μl of water (100 v/v)
    E1
    • Tacsimate 2 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    20 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    480 μl of water (100 v/v)
    E2
    • Tacsimate 2 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    480 μl of water (100 v/v)
    E3
    • Tacsimate 2 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 8.5
    20 μl of Tacsimate pH 7 (100 v/v)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of water (100 v/v)
    E4
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 3.4
    		pH 3.4
    0 μl of CBTP buffer pH 7.2 (1 M)
    100 μl of CBTP buffer pH 2.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    E5
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 4.1
    		pH 4.1
    0 μl of CBTP buffer pH 7.2 (1 M)
    99 μl of CBTP buffer pH 2.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    E6
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0 M pH 5
    		pH 5
    0 μl of CBTP buffer pH 7.2 (1 M)
    0 μl of CBTP buffer pH 2.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    680 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,350 20 w/v -
    • CBTP buffer 0 M pH 6.4
    		pH 6.4
    0 μl of CBTP buffer pH 7.2 (1 M)
    0 μl of CBTP buffer pH 2.2 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    E8
    • Polyethylene glycol 3,350 20 w/v -
    • CBTP buffer 0.1 M pH 7.6
    		pH 7.6
    18 μl of CBTP buffer pH 2.2 (1 M)
    81 μl of CBTP buffer pH 8.8 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 8.8
    		pH 8.8
    0 μl of CBTP buffer pH 2.2 (1 M)
    100 μl of CBTP buffer pH 8.8 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    F2
    • Calcium chloride 0.02 M -
    • Cadmium chloride 0.02 M -
    • Cobalt (II) chloride 0.02 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    20 μl of Cobalt (II) chloride (1 M)
    10 μl of Calcium chloride (2 M)
    20 μl of Cadmium chloride (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    F3
    • Magnesium chloride 0.01 M -
    • Nickel (II) chloride 0.005 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    50 μl of Nickel (II) chloride (0.1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    5 μl of Magnesium chloride (2 M)
    545 μl of water (100 v/v)
    F4
    • Zinc chloride 0.02 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    10 μl of Zinc chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    590 μl of water (100 v/v)
    F5
    • Cesium chloride 0.15 M -
    • Polyethylene glycol 3,350 15 w/v -
    		NA
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    150 μl of Cesium chloride (1 M)
    550 μl of water (100 v/v)
    F6
    • Sodium bromide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    F7
    • Tryptone 1 w/v -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 12 w/v -
    		pH 7
    50 μl of HEPES pH 7 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Tryptone (10 w/v)
    610 μl of water (100 v/v)
    F8
    • Tryptone 1 w/v -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 20 w/v -
    		pH 7
    50 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Tryptone (10 w/v)
    450 μl of water (100 v/v)