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Supplemental Information

Screen Description

PEG Ion Screen 2

Hampton Research / PEG Ion Screen 2
Created 2009-11-16.

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PEG Ion Screen 2

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PEG Ion Screen 2

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Screen Structure

PEG Ion Screen 2

Well Count 48
Number of distinct ingredients 24
Internal diversity (score mean dist) 0.659

Screen Composition

More information about screen composition chart.
2025-08-11T21:59:43.127620 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PEG Ion Screen 2
Buffer 48
Complex ingredient 15
Polymer 48
Salt 45

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG Ion Screen 2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 4 (3.4 M)
730.6 μl of water (100 v/v)
A2
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 4 (3.4 M)
541.2 μl of water (100 v/v)
A3
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 5 (3.4 M)
730.6 μl of water (100 v/v)
A4
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 5 (3.4 M)
541.2 μl of water (100 v/v)
A5
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 6 (3.4 M)
730.6 μl of water (100 v/v)
A6
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 6 (3.4 M)
541.2 μl of water (100 v/v)
A7
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 7 (3.4 M)
730.6 μl of water (100 v/v)
A8
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
541.2 μl of water (100 v/v)
B1
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
B2
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
B3
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
B4
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
B5
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
B6
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
B7
  • Tacsimate 12 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
120.0 μl of Tacsimate pH 7 (100 v/v)
640.0 μl of water (100 v/v)
B8
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
C1
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
C2
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
C3
  • di-Sodium succinate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
83.3 μl of di-Sodium succinate (1.2 M)
676.7 μl of water (100 v/v)
C4
  • di-Sodium succinate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
166.7 μl of di-Sodium succinate (1.2 M)
433.3 μl of water (100 v/v)
C5
  • tri-Ammonium citrate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of tri-Ammonium citrate (2.5 M)
720.0 μl of water (100 v/v)
C6
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
520.0 μl of water (100 v/v)
C7
  • di-Sodium DL-malate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of di-Sodium DL-malate (3 M)
726.7 μl of water (100 v/v)
C8
  • di-Sodium DL-malate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of di-Sodium DL-malate (3 M)
533.3 μl of water (100 v/v)
D1
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium acetate (4 M)
735.0 μl of water (100 v/v)
D2
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate (4 M)
550.0 μl of water (100 v/v)
D3
  • Sodium formate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Sodium formate (5 M)
740.0 μl of water (100 v/v)
D4
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium formate (5 M)
560.0 μl of water (100 v/v)
D5
  • di-Ammonium tartrate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
62.5 μl of di-Ammonium tartrate (1.6 M)
697.5 μl of water (100 v/v)
D6
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of di-Ammonium tartrate (1.6 M)
475.0 μl of water (100 v/v)
D7
  • Tacsimate 2 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 3,350 16 w/v -
pH 4.6
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Tacsimate pH 7 (100 v/v)
560.0 μl of water (100 v/v)
D8
  • Tacsimate 2 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 3,350 16 w/v -
pH 5.6
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
560.0 μl of water (100 v/v)
E1
  • Tacsimate 2 v/v -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
480.0 μl of water (100 v/v)
E2
  • Tacsimate 2 v/v -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
480.0 μl of water (100 v/v)
E3
  • Tacsimate 2 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 3.4
pH 3.4
100.0 μl of CBTP buffer pH 2.2 (1 M)
0.0 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 4.1
pH 4.1
99.8 μl of CBTP buffer pH 2.2 (1 M)
0.2 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0 M pH 5
pH 5
0.0 μl of CBTP buffer pH 2.2 (1 M)
0.0 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
680.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 3,350 20 w/v -
  • CBTP buffer 0 M pH 6.4
pH 6.4
0.0 μl of CBTP buffer pH 2.2 (1 M)
0.0 μl of CBTP buffer pH 7.2 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 3,350 20 w/v -
  • CBTP buffer 0.1 M pH 7.6
pH 7.6
81.2 μl of CBTP buffer pH 8.8 (1 M)
18.8 μl of CBTP buffer pH 2.2 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 8.8
pH 8.8
100.0 μl of CBTP buffer pH 8.8 (1 M)
-0.0 μl of CBTP buffer pH 2.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
F2
  • Calcium chloride 0.02 M -
  • Cadmium chloride 0.02 M -
  • Cobalt (II) chloride 0.02 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Cadmium chloride (1 M)
10.0 μl of Calcium chloride (2 M)
20.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
F3
  • Magnesium chloride 0.01 M -
  • Nickel (II) chloride 0.005 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
5.0 μl of Magnesium chloride (2 M)
50.0 μl of Nickel (II) chloride (0.1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
545.0 μl of water (100 v/v)
F4
  • Zinc chloride 0.02 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Zinc chloride (2 M)
590.0 μl of water (100 v/v)
F5
  • Cesium chloride 0.15 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
150.0 μl of Cesium chloride (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
F6
  • Sodium bromide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium bromide (5 M)
560.0 μl of water (100 v/v)
F7
  • Tryptone 1 w/v -
  • HEPES 0.05 M pH 7
  • Polyethylene glycol 3,350 12 w/v -
pH 7
50.0 μl of HEPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Tryptone (10 w/v)
610.0 μl of water (100 v/v)
F8
  • Tryptone 1 w/v -
  • HEPES 0.05 M pH 7
  • Polyethylene glycol 3,350 20 w/v -
pH 7
50.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Tryptone (10 w/v)
450.0 μl of water (100 v/v)