Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Jump to well contents.

Supplemental Information

Screen Description

PS gradient - mid range

BCC Core Screens / PS gradient - mid range
Created 2025-03-06.
pH and salt gradient: The salt (1.2 - 2.2M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

Export

PS gradient - mid range

Barcodes

PS gradient - mid range

  • Z0002252 Lot 1 (1500.0 µl)
  • Z0002681 Lot 2 (1500.0 µl)

Screen Structure

PS gradient - mid range

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.741

Screen Composition

More information about screen composition chart.
2025-08-15T23:26:01.849380 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PS gradient - mid range
Buffer 132
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PS gradient - mid range Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 1.2 M -
NA
342.9 μl of ammonium sulfate BCC (3.5 M)
657.1 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1.4 M -
NA
400.0 μl of ammonium sulfate BCC (3.5 M)
600.0 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 1.6 M -
NA
457.1 μl of ammonium sulfate BCC (3.5 M)
542.9 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 1.8 M -
NA
514.3 μl of ammonium sulfate BCC (3.5 M)
485.7 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
428.6 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2.2 M -
NA
628.6 μl of ammonium sulfate BCC (3.5 M)
371.4 μl of water (100 v/v)
A7
  • sodium malonate-malonic acid BCC 1.2 M -
NA
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
647.1 μl of water (100 v/v)
A8
  • sodium malonate-malonic acid BCC 1.4 M -
NA
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
588.2 μl of water (100 v/v)
A9
  • sodium malonate-malonic acid BCC 1.6 M -
NA
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
529.4 μl of water (100 v/v)
A10
  • sodium malonate-malonic acid BCC 1.8 M -
NA
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
470.6 μl of water (100 v/v)
A11
  • sodium malonate-malonic acid BCC 2 M -
NA
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
411.8 μl of water (100 v/v)
A12
  • sodium malonate-malonic acid BCC 2.2 M -
NA
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
352.9 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
B7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
B8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
B9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
B10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
B11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
B12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
C7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
C8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
C9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
C10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
C11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
C12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
D7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
D8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
D9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
D10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
D11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 1.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
342.9 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
557.1 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 1.4 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 1.6 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
457.1 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
442.9 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 1.8 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
514.3 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
385.7 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
328.6 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 2.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
628.6 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
271.4 μl of water (100 v/v)
E7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 5.5
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
547.1 μl of water (100 v/v)
E8
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 5.5
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
488.2 μl of water (100 v/v)
E9
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 5.5
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
429.4 μl of water (100 v/v)
E10
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 5.5
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
370.6 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2 M -
pH 5.5
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
311.8 μl of water (100 v/v)
E12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 5.5
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
252.9 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
342.9 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
557.1 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
500.0 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
457.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
442.9 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
514.3 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
385.7 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
328.6 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
628.6 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
271.4 μl of water (100 v/v)
F7
  • sodium malonate-malonic acid BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
F8
  • sodium malonate-malonic acid BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
F9
  • sodium malonate-malonic acid BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
F10
  • sodium malonate-malonic acid BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
F11
  • sodium malonate-malonic acid BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
F12
  • sodium malonate-malonic acid BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 1.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
342.9 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
557.1 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 1.4 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 1.6 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457.1 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
442.9 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 1.8 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
514.3 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
385.7 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 2.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
628.6 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
271.4 μl of water (100 v/v)
G7
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
G10
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
G11
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
H1
  • ammonium sulfate BCC 1.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
342.9 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
557.1 μl of water (100 v/v)
H2
  • ammonium sulfate BCC 1.4 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
500.0 μl of water (100 v/v)
H3
  • ammonium sulfate BCC 1.6 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
457.1 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
442.9 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 1.8 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
514.3 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
385.7 μl of water (100 v/v)
H5
  • ammonium sulfate BCC 2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
328.6 μl of water (100 v/v)
H6
  • ammonium sulfate BCC 2.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
628.6 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
271.4 μl of water (100 v/v)
H7
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
H8
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
H9
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
H10
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
H11
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
H12
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)