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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
PS gradient - mid range
BCC Core Screens / PS gradient - mid range
Created 2025-03-06.
pH and salt gradient: The salt (1.2 - 2.2M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).
Export
PS gradient - mid range
Barcodes
PS gradient - mid range
- Z0002252
- Z0002681
Screen Structure
PS gradient - mid range
Well Count | 96 |
---|---|
Number of distinct ingredients | 7 |
Internal diversity (score mean dist) | 0.741 |
Screen Composition
ingredient type | PS gradient - mid range |
---|---|
Buffer | 132 |
Salt | 108 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 342 μl of ammonium sulfate BCC (3.5 M) 657 μl of water (100 v/v) |
A2 |
| NA | 400 μl of ammonium sulfate BCC (3.5 M) 600 μl of water (100 v/v) |
A3 |
| NA | 457 μl of ammonium sulfate BCC (3.5 M) 542 μl of water (100 v/v) |
A4 |
| NA | 514 μl of ammonium sulfate BCC (3.5 M) 485 μl of water (100 v/v) |
A5 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 428 μl of water (100 v/v) |
A6 |
| NA | 628 μl of ammonium sulfate BCC (3.5 M) 371 μl of water (100 v/v) |
A7 |
| NA | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 647 μl of water (100 v/v) |
A8 |
| NA | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 588 μl of water (100 v/v) |
A9 |
| NA | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 529 μl of water (100 v/v) |
A10 |
| NA | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 470 μl of water (100 v/v) |
A11 |
| NA | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 411 μl of water (100 v/v) |
A12 |
| NA | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 352 μl of water (100 v/v) |
B1 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 342 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 557 μl of water (100 v/v) |
B2 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 400 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of water (100 v/v) |
B3 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 457 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 442 μl of water (100 v/v) |
B4 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 514 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 385 μl of water (100 v/v) |
B5 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 571 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 328 μl of water (100 v/v) |
B6 |
| pH 5 | 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 628 μl of ammonium sulfate BCC (3.5 M) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 271 μl of water (100 v/v) |
B7 |
| pH 5 | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 547 μl of water (100 v/v) |
B8 |
| pH 5 | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 488 μl of water (100 v/v) |
B9 |
| pH 5 | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 429 μl of water (100 v/v) |
B10 |
| pH 5 | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 370 μl of water (100 v/v) |
B11 |
| pH 5 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
B12 |
| pH 5 | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 252 μl of water (100 v/v) |
C1 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 342 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 557 μl of water (100 v/v) |
C2 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 400 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of water (100 v/v) |
C3 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 457 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 442 μl of water (100 v/v) |
C4 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 514 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 385 μl of water (100 v/v) |
C5 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 571 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 328 μl of water (100 v/v) |
C6 |
| pH 7 | 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 628 μl of ammonium sulfate BCC (3.5 M) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 271 μl of water (100 v/v) |
C7 |
| pH 7 | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 547 μl of water (100 v/v) |
C8 |
| pH 7 | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 488 μl of water (100 v/v) |
C9 |
| pH 7 | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 429 μl of water (100 v/v) |
C10 |
| pH 7 | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 370 μl of water (100 v/v) |
C11 |
| pH 7 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
C12 |
| pH 7 | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v) 60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 252 μl of water (100 v/v) |
D1 |
| pH 9 | 342 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 557 μl of water (100 v/v) |
D2 |
| pH 9 | 400 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 500 μl of water (100 v/v) |
D3 |
| pH 9 | 457 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 442 μl of water (100 v/v) |
D4 |
| pH 9 | 514 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 385 μl of water (100 v/v) |
D5 |
| pH 9 | 571 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 328 μl of water (100 v/v) |
D6 |
| pH 9 | 628 μl of ammonium sulfate BCC (3.5 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 271 μl of water (100 v/v) |
D7 |
| pH 9 | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 547 μl of water (100 v/v) |
D8 |
| pH 9 | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 488 μl of water (100 v/v) |
D9 |
| pH 9 | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 429 μl of water (100 v/v) |
D10 |
| pH 9 | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 370 μl of water (100 v/v) |
D11 |
| pH 9 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 311 μl of water (100 v/v) |
D12 |
| pH 9 | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v) 252 μl of water (100 v/v) |
E1 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 342 μl of ammonium sulfate BCC (3.5 M) 557 μl of water (100 v/v) |
E2 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 400 μl of ammonium sulfate BCC (3.5 M) 500 μl of water (100 v/v) |
E3 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 442 μl of water (100 v/v) |
E4 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 514 μl of ammonium sulfate BCC (3.5 M) 385 μl of water (100 v/v) |
E5 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
E6 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 628 μl of ammonium sulfate BCC (3.5 M) 271 μl of water (100 v/v) |
E7 |
| pH 5.5 | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 547 μl of water (100 v/v) |
E8 |
| pH 5.5 | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 488 μl of water (100 v/v) |
E9 |
| pH 5.5 | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 429 μl of water (100 v/v) |
E10 |
| pH 5.5 | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 370 μl of water (100 v/v) |
E11 |
| pH 5.5 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 311 μl of water (100 v/v) |
E12 |
| pH 5.5 | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 252 μl of water (100 v/v) |
F1 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 342 μl of ammonium sulfate BCC (3.5 M) 557 μl of water (100 v/v) |
F2 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 400 μl of ammonium sulfate BCC (3.5 M) 500 μl of water (100 v/v) |
F3 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 442 μl of water (100 v/v) |
F4 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 514 μl of ammonium sulfate BCC (3.5 M) 385 μl of water (100 v/v) |
F5 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
F6 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 628 μl of ammonium sulfate BCC (3.5 M) 271 μl of water (100 v/v) |
F7 |
| pH 6.5 | 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 547 μl of water (100 v/v) |
F8 |
| pH 6.5 | 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 488 μl of water (100 v/v) |
F9 |
| pH 6.5 | 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 429 μl of water (100 v/v) |
F10 |
| pH 6.5 | 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 370 μl of water (100 v/v) |
F11 |
| pH 6.5 | 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 311 μl of water (100 v/v) |
F12 |
| pH 6.5 | 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 252 μl of water (100 v/v) |
G1 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 342 μl of ammonium sulfate BCC (3.5 M) 557 μl of water (100 v/v) |
G2 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of ammonium sulfate BCC (3.5 M) 500 μl of water (100 v/v) |
G3 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 442 μl of water (100 v/v) |
G4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 514 μl of ammonium sulfate BCC (3.5 M) 385 μl of water (100 v/v) |
G5 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
G6 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 628 μl of ammonium sulfate BCC (3.5 M) 271 μl of water (100 v/v) |
G7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 547 μl of water (100 v/v) |
G8 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 488 μl of water (100 v/v) |
G9 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 429 μl of water (100 v/v) |
G10 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 370 μl of water (100 v/v) |
G11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 311 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 252 μl of water (100 v/v) |
H1 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 342 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 557 μl of water (100 v/v) |
H2 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 400 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 500 μl of water (100 v/v) |
H3 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 442 μl of water (100 v/v) |
H4 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 514 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 385 μl of water (100 v/v) |
H5 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 328 μl of water (100 v/v) |
H6 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 628 μl of ammonium sulfate BCC (3.5 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 271 μl of water (100 v/v) |
H7 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 547 μl of water (100 v/v) |
H8 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 488 μl of water (100 v/v) |
H9 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 429 μl of water (100 v/v) |
H10 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 370 μl of water (100 v/v) |
H11 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 311 μl of water (100 v/v) |
H12 |
| pH 8.5 | 34 μl of gly-gly BCC pH 7.3 (1 M) 647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 66 μl of gly-gly BCC pH 9.3 (1 M) 252 μl of water (100 v/v) |