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Supplemental Information

Screen Description

PS gradient - mid range

BCC Core Screens / PS gradient - mid range
Created 2025-03-06.
pH and salt gradient: The salt (1.2 - 2.2M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

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PS gradient - mid range

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PS gradient - mid range

  • Z0002252 Lot 1 (1500.0 µl)
  • Z0002681 - 20/6/22 Lot 2 (1500.0 µl)

Screen Structure

PS gradient - mid range

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.741

Screen Composition

More information about screen composition chart.
2025-08-11T21:25:55.425822 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PS gradient - mid range
Buffer 132
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PS gradient - mid range Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 1.2 M -
NA
342.9 μl of ammonium sulfate BCC (3.5 M)
657.1 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1.4 M -
NA
400.0 μl of ammonium sulfate BCC (3.5 M)
600.0 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 1.6 M -
NA
457.1 μl of ammonium sulfate BCC (3.5 M)
542.9 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 1.8 M -
NA
514.3 μl of ammonium sulfate BCC (3.5 M)
485.7 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
428.6 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2.2 M -
NA
628.6 μl of ammonium sulfate BCC (3.5 M)
371.4 μl of water (100 v/v)
A7
  • sodium malonate-malonic acid BCC 1.2 M -
NA
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
647.1 μl of water (100 v/v)
A8
  • sodium malonate-malonic acid BCC 1.4 M -
NA
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
588.2 μl of water (100 v/v)
A9
  • sodium malonate-malonic acid BCC 1.6 M -
NA
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
529.4 μl of water (100 v/v)
A10
  • sodium malonate-malonic acid BCC 1.8 M -
NA
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
470.6 μl of water (100 v/v)
A11
  • sodium malonate-malonic acid BCC 2 M -
NA
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
411.8 μl of water (100 v/v)
A12
  • sodium malonate-malonic acid BCC 2.2 M -
NA
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
352.9 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
B7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
B8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
B9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
B10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
B11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
B12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
C7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
C8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
C9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
C10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
C11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
C12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40.0 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
342.9 μl of ammonium sulfate BCC (3.5 M)
557.1 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
400.0 μl of ammonium sulfate BCC (3.5 M)
500.0 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
457.1 μl of ammonium sulfate BCC (3.5 M)
442.9 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
514.3 μl of ammonium sulfate BCC (3.5 M)
385.7 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
628.6 μl of ammonium sulfate BCC (3.5 M)
271.4 μl of water (100 v/v)
D7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
D8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
D9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
D10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
D11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
100.0 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 1.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
342.9 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
557.1 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 1.4 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 1.6 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
457.1 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
442.9 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 1.8 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
514.3 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
385.7 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
328.6 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 2.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
628.6 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
271.4 μl of water (100 v/v)
E7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 5.5
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
547.1 μl of water (100 v/v)
E8
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 5.5
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
488.2 μl of water (100 v/v)
E9
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 5.5
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
429.4 μl of water (100 v/v)
E10
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 5.5
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
370.6 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2 M -
pH 5.5
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
311.8 μl of water (100 v/v)
E12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 5.5
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
252.9 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
342.9 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
557.1 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
500.0 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
457.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
442.9 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
514.3 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
385.7 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
328.6 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
628.6 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
271.4 μl of water (100 v/v)
F7
  • sodium malonate-malonic acid BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
F8
  • sodium malonate-malonic acid BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
F9
  • sodium malonate-malonic acid BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
F10
  • sodium malonate-malonic acid BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
F11
  • sodium malonate-malonic acid BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
F12
  • sodium malonate-malonic acid BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 1.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
342.9 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
557.1 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 1.4 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 1.6 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457.1 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
442.9 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 1.8 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
514.3 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
385.7 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 2.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
628.6 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
271.4 μl of water (100 v/v)
G7
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
G10
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
G11
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)
H1
  • ammonium sulfate BCC 1.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
342.9 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
557.1 μl of water (100 v/v)
H2
  • ammonium sulfate BCC 1.4 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
500.0 μl of water (100 v/v)
H3
  • ammonium sulfate BCC 1.6 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
457.1 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
442.9 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 1.8 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
514.3 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
385.7 μl of water (100 v/v)
H5
  • ammonium sulfate BCC 2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
328.6 μl of water (100 v/v)
H6
  • ammonium sulfate BCC 2.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
628.6 μl of ammonium sulfate BCC (3.5 M)
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
271.4 μl of water (100 v/v)
H7
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
352.9 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
547.1 μl of water (100 v/v)
H8
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
411.8 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
488.2 μl of water (100 v/v)
H9
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
470.6 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
429.4 μl of water (100 v/v)
H10
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
529.4 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
370.6 μl of water (100 v/v)
H11
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
588.2 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
311.8 μl of water (100 v/v)
H12
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 8.5
34.0 μl of gly-gly BCC pH 7.3 (1 M)
66.0 μl of gly-gly BCC pH 9.3 (1 M)
647.1 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
252.9 μl of water (100 v/v)