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Supplemental Information

Screen Description

PS gradient - mid range

BCC Core Screens / PS gradient - mid range
Created 2025-03-06.
pH and salt gradient: The salt (1.2 - 2.2M) is either ammonium sulfate or sodium malonate, pH ranges from 4 - 10 (one buffer system), and 5.5, 6.5 ,7.5 or 8.5 (distinct buffers).

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Barcodes

PS gradient - mid range

  • Z0002252
  • Z0002681

Screen Structure

PS gradient - mid range

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.741

Screen Composition

2025-06-12T23:03:02.015785 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type PS gradient - mid range
Buffer 132
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PS gradient - mid range Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 1.2 M -
NA
342 μl of ammonium sulfate BCC (3.5 M)
657 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1.4 M -
NA
400 μl of ammonium sulfate BCC (3.5 M)
600 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 1.6 M -
NA
457 μl of ammonium sulfate BCC (3.5 M)
542 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 1.8 M -
NA
514 μl of ammonium sulfate BCC (3.5 M)
485 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
428 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2.2 M -
NA
628 μl of ammonium sulfate BCC (3.5 M)
371 μl of water (100 v/v)
A7
  • sodium malonate-malonic acid BCC 1.2 M -
NA
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
647 μl of water (100 v/v)
A8
  • sodium malonate-malonic acid BCC 1.4 M -
NA
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
588 μl of water (100 v/v)
A9
  • sodium malonate-malonic acid BCC 1.6 M -
NA
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
529 μl of water (100 v/v)
A10
  • sodium malonate-malonic acid BCC 1.8 M -
NA
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
470 μl of water (100 v/v)
A11
  • sodium malonate-malonic acid BCC 2 M -
NA
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
411 μl of water (100 v/v)
A12
  • sodium malonate-malonic acid BCC 2.2 M -
NA
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
352 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
342 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
557 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
400 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
457 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
442 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
514 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
385 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
571 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
328 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
628 μl of ammonium sulfate BCC (3.5 M)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
271 μl of water (100 v/v)
B7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
547 μl of water (100 v/v)
B8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
488 μl of water (100 v/v)
B9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
429 μl of water (100 v/v)
B10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
370 μl of water (100 v/v)
B11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
B12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 5
pH 5
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
80 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
20 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
252 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
342 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
557 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
400 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
457 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
442 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
514 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
385 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
571 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
328 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
628 μl of ammonium sulfate BCC (3.5 M)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
271 μl of water (100 v/v)
C7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
547 μl of water (100 v/v)
C8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
488 μl of water (100 v/v)
C9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
429 μl of water (100 v/v)
C10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
370 μl of water (100 v/v)
C11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
C12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 7
pH 7
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
40 μl of DL-malate-MES-tris BCC pH 4 (100 v/v)
60 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
252 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
342 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
557 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
400 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
500 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
457 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
442 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
514 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
385 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
571 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
328 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
628 μl of ammonium sulfate BCC (3.5 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
271 μl of water (100 v/v)
D7
  • sodium malonate-malonic acid BCC 1.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
547 μl of water (100 v/v)
D8
  • sodium malonate-malonic acid BCC 1.4 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
488 μl of water (100 v/v)
D9
  • sodium malonate-malonic acid BCC 1.6 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
429 μl of water (100 v/v)
D10
  • sodium malonate-malonic acid BCC 1.8 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
370 μl of water (100 v/v)
D11
  • sodium malonate-malonic acid BCC 2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
311 μl of water (100 v/v)
D12
  • sodium malonate-malonic acid BCC 2.2 M -
  • DL-malate-MES-tris BCC 10 v/v pH 9
pH 9
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of DL-malate-MES-tris BCC pH 9 (100 v/v)
252 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 1.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
342 μl of ammonium sulfate BCC (3.5 M)
557 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 1.4 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
400 μl of ammonium sulfate BCC (3.5 M)
500 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 1.6 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
442 μl of water (100 v/v)
E4
  • ammonium sulfate BCC 1.8 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
514 μl of ammonium sulfate BCC (3.5 M)
385 μl of water (100 v/v)
E5
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 2.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
628 μl of ammonium sulfate BCC (3.5 M)
271 μl of water (100 v/v)
E7
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 5.5
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
547 μl of water (100 v/v)
E8
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 5.5
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
488 μl of water (100 v/v)
E9
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 5.5
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
429 μl of water (100 v/v)
E10
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 5.5
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
370 μl of water (100 v/v)
E11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2 M -
pH 5.5
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
311 μl of water (100 v/v)
E12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 5.5
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
252 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
342 μl of ammonium sulfate BCC (3.5 M)
557 μl of water (100 v/v)
F2
  • ammonium sulfate BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
400 μl of ammonium sulfate BCC (3.5 M)
500 μl of water (100 v/v)
F3
  • ammonium sulfate BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
442 μl of water (100 v/v)
F4
  • ammonium sulfate BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
514 μl of ammonium sulfate BCC (3.5 M)
385 μl of water (100 v/v)
F5
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
628 μl of ammonium sulfate BCC (3.5 M)
271 μl of water (100 v/v)
F7
  • sodium malonate-malonic acid BCC 1.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
547 μl of water (100 v/v)
F8
  • sodium malonate-malonic acid BCC 1.4 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
488 μl of water (100 v/v)
F9
  • sodium malonate-malonic acid BCC 1.6 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
429 μl of water (100 v/v)
F10
  • sodium malonate-malonic acid BCC 1.8 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
370 μl of water (100 v/v)
F11
  • sodium malonate-malonic acid BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
311 μl of water (100 v/v)
F12
  • sodium malonate-malonic acid BCC 2.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
252 μl of water (100 v/v)
G1
  • ammonium sulfate BCC 1.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
342 μl of ammonium sulfate BCC (3.5 M)
557 μl of water (100 v/v)
G2
  • ammonium sulfate BCC 1.4 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
400 μl of ammonium sulfate BCC (3.5 M)
500 μl of water (100 v/v)
G3
  • ammonium sulfate BCC 1.6 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
442 μl of water (100 v/v)
G4
  • ammonium sulfate BCC 1.8 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
514 μl of ammonium sulfate BCC (3.5 M)
385 μl of water (100 v/v)
G5
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
G6
  • ammonium sulfate BCC 2.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
628 μl of ammonium sulfate BCC (3.5 M)
271 μl of water (100 v/v)
G7
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
547 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
488 μl of water (100 v/v)
G9
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
429 μl of water (100 v/v)
G10
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
370 μl of water (100 v/v)
G11
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
311 μl of water (100 v/v)
G12
  • sodium HEPES BCC 0.1 M pH 7.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 7.5
50 μl of sodium HEPES BCC pH 8.5 (1 M)
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
252 μl of water (100 v/v)
H1
  • ammonium sulfate BCC 1.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
342 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
557 μl of water (100 v/v)
H2
  • ammonium sulfate BCC 1.4 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
400 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
500 μl of water (100 v/v)
H3
  • ammonium sulfate BCC 1.6 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
442 μl of water (100 v/v)
H4
  • ammonium sulfate BCC 1.8 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
514 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
385 μl of water (100 v/v)
H5
  • ammonium sulfate BCC 2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
328 μl of water (100 v/v)
H6
  • ammonium sulfate BCC 2.2 M -
  • gly-gly BCC 0.1 M pH 8.5
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
628 μl of ammonium sulfate BCC (3.5 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
271 μl of water (100 v/v)
H7
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.2 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
352 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
547 μl of water (100 v/v)
H8
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.4 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
411 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
488 μl of water (100 v/v)
H9
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.6 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
470 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
429 μl of water (100 v/v)
H10
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 1.8 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
529 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
370 μl of water (100 v/v)
H11
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
588 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
311 μl of water (100 v/v)
H12
  • gly-gly BCC 0.1 M pH 8.5
  • sodium malonate-malonic acid BCC 2.2 M -
pH 8.5
34 μl of gly-gly BCC pH 7.3 (1 M)
647 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
66 μl of gly-gly BCC pH 9.3 (1 M)
252 μl of water (100 v/v)