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Supplemental Information

Screen Description

PEG Ion Screen HT

Hampton Research / PEG Ion Screen HT
Created 2009-11-16.

Export

PEG Ion Screen HT

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PEG Ion Screen HT

    Screen Structure

    PEG Ion Screen HT

    Well Count 96
    Number of distinct ingredients 68
    Internal diversity (score mean dist) 0.689

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:23:15.282323 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Ion Screen HT
    Buffer 70
    Complex ingredient 15
    Polymer 96
    Salt 95

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Ion Screen HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    		NA
    250 μl of Sodium fluoride (0.8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    350 μl of water (100 v/v)
    A2
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium fluoride 0.2 M -
    		NA
    33 μl of Potassium fluoride (6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    566 μl of water (100 v/v)
    A3
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium fluoride 0.2 M -
    		NA
    20 μl of Ammonium fluoride (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    A4
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium chloride 0.2 M -
    		NA
    20 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium chloride 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    500 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium chloride 0.2 M -
    		NA
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    A7
    • Polyethylene glycol 3,350 20 w/v -
    • Calcium chloride 0.2 M -
    		NA
    100 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    A8
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium chloride 0.2 M -
    		NA
    50 μl of Potassium chloride (4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    A9
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium chloride 0.2 M -
    		NA
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    A10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    		NA
    200 μl of Sodium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A11
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium iodide 0.2 M -
    		NA
    200 μl of Potassium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A12
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium iodide 0.2 M -
    		NA
    100 μl of Ammonium iodide (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    B1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium thiocyanate 0.2 M -
    		NA
    25 μl of Sodium thiocyanate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    B2
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    		NA
    25 μl of Potassium thiocyanate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium nitrate 0.2 M -
    		NA
    25 μl of Lithium nitrate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium nitrate 0.2 M -
    		NA
    66 μl of Magnesium nitrate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    B5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    		NA
    28 μl of Sodium nitrate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    B6
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium nitrate 0.2 M -
    		NA
    66 μl of Potassium nitrate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium nitrate 0.2 M -
    		NA
    20 μl of Ammonium nitrate (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B8
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium formate 0.2 M -
    		NA
    200 μl of Magnesium formate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    B9
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    		NA
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    B10
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium formate 0.2 M -
    		NA
    14 μl of Potassium formate (14 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    585 μl of water (100 v/v)
    B11
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium formate 0.2 M -
    		NA
    20 μl of Ammonium formate (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B12
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium acetate 0.2 M -
    		NA
    40 μl of Lithium acetate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    C1
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium acetate 0.2 M -
    		NA
    200 μl of Magnesium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C2
    • Polyethylene glycol 3,350 20 w/v -
    • Zinc acetate 0.2 M -
    		NA
    200 μl of Zinc acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Sodium acetate (3 M)
    533 μl of water (100 v/v)
    C4
    • Polyethylene glycol 3,350 20 w/v -
    • Calcium acetate 0.2 M -
    		NA
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C5
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium acetate 0.2 M -
    		NA
    40 μl of Potassium acetate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    C6
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium acetate 0.2 M -
    		NA
    200 μl of Ammonium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C7
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium sulfate 0.2 M -
    		NA
    100 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    C8
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium sulfate 0.2 M -
    		NA
    100 μl of Magnesium sulfate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    C9
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    		NA
    200 μl of Sodium sulfate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C10
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium sulfate 0.2 M -
    		NA
    400 μl of Potassium sulfate (0.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    C11
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    57 μl of Ammonium sulfate (3.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    542 μl of water (100 v/v)
    C12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium tartrate 0.2 M -
    		NA
    133 μl of di-Sodium tartrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    D1
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    		NA
    133 μl of Potassium Sodium tartrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    D2
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium tartrate 0.2 M -
    		NA
    100 μl of di-Ammonium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    D3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium di-hydrogen phosphate 0.2 M -
    		NA
    50 μl of Sodium di-hydrogen phosphate (4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    D4
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium hydrogen phosphate 0.2 M -
    		NA
    200 μl of di-Sodium hydrogen phosphate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium di-hydrogen phosphate 0.2 M -
    		NA
    133 μl of Potassium di-hydrogen phosphate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    D6
    • Polyethylene glycol 3,350 20 w/v -
    • di-Potassium hydrogen phosphate 0.2 M -
    		NA
    50 μl of di-Potassium hydrogen phosphate (4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    D7
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium di-hydrogen phosphate 0.2 M -
    		NA
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    D8
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium hydrogen phosphate 0.2 M -
    		NA
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    542 μl of water (100 v/v)
    D9
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Lithium citrate 0.2 M -
    		NA
    133 μl of tri-Lithium citrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    D10
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    		NA
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    D11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Potassium citrate 0.2 M -
    		NA
    80 μl of tri-Potassium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    D12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium hydrogen citrate 0.2 M -
    		NA
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    E1
    • di-Sodium malonate 0.1 M pH 4
    • Polyethylene glycol 3,350 12 w/v -
    		pH 4
    29 μl of di-Sodium malonate pH 4 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    E2
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 4 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    E3
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 5 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    E4
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 5 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    E5
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 6 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    E6
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 6 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    E7
    • di-Sodium malonate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    29 μl of di-Sodium malonate pH 7 (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    730 μl of water (100 v/v)
    E8
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    E9
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    E10
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    E11
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    E12
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F1
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    F2
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F3
    • Tacsimate 12 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    120 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    640 μl of water (100 v/v)
    F4
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F5
    • Tacsimate 4 v/v -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of Tacsimate pH 7 (100 v/v)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    F6
    • Tacsimate 8 v/v -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F7
    • di-Sodium succinate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    83 μl of di-Sodium succinate (1.2 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    676 μl of water (100 v/v)
    F8
    • di-Sodium succinate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    166 μl of di-Sodium succinate (1.2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    433 μl of water (100 v/v)
    F9
    • tri-Ammonium citrate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    40 μl of tri-Ammonium citrate (2.5 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    F10
    • tri-Ammonium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of tri-Ammonium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F11
    • di-Sodium DL-malate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    33 μl of di-Sodium DL-malate (3 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    726 μl of water (100 v/v)
    F12
    • di-Sodium DL-malate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    66 μl of di-Sodium DL-malate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    G1
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    25 μl of Sodium acetate (4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    735 μl of water (100 v/v)
    G2
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    50 μl of Sodium acetate (4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    G3
    • Sodium formate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    20 μl of Sodium formate (5 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    740 μl of water (100 v/v)
    G4
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    40 μl of Sodium formate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    G5
    • di-Ammonium tartrate 0.1 M -
    • Polyethylene glycol 3,350 12 w/v -
    		NA
    62 μl of di-Ammonium tartrate (1.6 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    697 μl of water (100 v/v)
    G6
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    125 μl of di-Ammonium tartrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    G7
    • Tacsimate 2 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 3,350 16 w/v -
    		pH 4.6
    20 μl of Tacsimate pH 7 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    G8
    • Tacsimate 2 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 3,350 16 w/v -
    		pH 5.6
    20 μl of Tacsimate pH 7 (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    G9
    • Tacsimate 2 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 6.5
    20 μl of Tacsimate pH 7 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    480 μl of water (100 v/v)
    G10
    • Tacsimate 2 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 7.5
    20 μl of Tacsimate pH 7 (100 v/v)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of water (100 v/v)
    G11
    • Tacsimate 2 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 8.5
    20 μl of Tacsimate pH 7 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    G12
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 3.4
    		pH 3.4
    100 μl of CBTP buffer pH 2.2 (1 M)
    0 μl of CBTP buffer pH 7.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    H1
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 4.1
    		pH 4.1
    99 μl of CBTP buffer pH 2.2 (1 M)
    0 μl of CBTP buffer pH 7.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    H2
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 5
    		pH 5
    98 μl of CBTP buffer pH 2.2 (1 M)
    1 μl of CBTP buffer pH 7.2 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    H3
    • Polyethylene glycol 3,350 20 w/v -
    • CBTP buffer 0.1 M pH 6.4
    		pH 6.4
    67 μl of CBTP buffer pH 2.2 (1 M)
    32 μl of CBTP buffer pH 7.2 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    H4
    • Polyethylene glycol 3,350 20 w/v -
    • CBTP buffer 0.1 M pH 7.6
    		pH 7.6
    18 μl of CBTP buffer pH 2.2 (1 M)
    81 μl of CBTP buffer pH 8.8 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    H5
    • Polyethylene glycol 3,350 16 w/v -
    • CBTP buffer 0.1 M pH 8.8
    		pH 8.8
    0 μl of CBTP buffer pH 2.2 (1 M)
    100 μl of CBTP buffer pH 8.8 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    H6
    • Calcium chloride 0.02 M -
    • Cadmium chloride 0.02 M -
    • Cobalt (II) chloride 0.02 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    20 μl of Cobalt (II) chloride (1 M)
    20 μl of Cadmium chloride (1 M)
    10 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    H7
    • Magnesium chloride 0.01 M -
    • Nickel (II) chloride 0.005 M -
    • Polyethylene glycol 3,350 15 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    50 μl of Nickel (II) chloride (0.1 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    5 μl of Magnesium chloride (2 M)
    545 μl of water (100 v/v)
    H8
    • Zinc chloride 0.02 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    10 μl of Zinc chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    590 μl of water (100 v/v)
    H9
    • Cesium chloride 0.15 M -
    • Polyethylene glycol 3,350 15 w/v -
    		NA
    150 μl of Cesium chloride (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    550 μl of water (100 v/v)
    H10
    • Sodium bromide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    H11
    • Tryptone 1 w/v -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 12 w/v -
    • Sodium azide 1 mM -
    		pH 7
    100 μl of Tryptone (10 w/v)
    50 μl of HEPES pH 7 (1 M)
    10 μl of Sodium azide (100 mM)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    H12
    • Tryptone 1 w/v -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium azide 1 mM -
    		pH 7
    100 μl of Tryptone (10 w/v)
    50 μl of HEPES pH 7 (1 M)
    10 μl of Sodium azide (100 mM)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    440 μl of water (100 v/v)