Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

PEG Ion Screen HT

Hampton Research / PEG Ion Screen HT
Created 2009-11-16.

Export

Barcodes

PEG Ion Screen HT

No barcodes found.

Screen Structure

PEG Ion Screen HT

Well Count 96
Number of distinct ingredients 68
Internal diversity (score mean dist) 0.689

Screen Composition

2025-08-11T22:48:56.970496 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PEG Ion Screen HT
Buffer 70
Complex ingredient 15
Polymer 96
Salt 95

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG Ion Screen HT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Potassium fluoride (6 M)
566.7 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium fluoride 0.2 M -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium chloride 0.2 M -
NA
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium chloride 0.2 M -
NA
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium chloride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 3,350 20 w/v -
  • Calcium chloride 0.2 M -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium chloride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium iodide 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium iodide (1 M)
400.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium iodide 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium iodide (1 M)
400.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium iodide 0.2 M -
NA
100.0 μl of Ammonium iodide (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium nitrate 0.2 M -
NA
25.0 μl of Lithium nitrate (8 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
575.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium nitrate 0.2 M -
NA
66.7 μl of Magnesium nitrate (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
533.3 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium nitrate (7 M)
571.4 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate (3 M)
533.3 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
B10
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.3 μl of Potassium formate (14 M)
585.7 μl of water (100 v/v)
B11
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium acetate 0.2 M -
NA
40.0 μl of Lithium acetate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium acetate 0.2 M -
NA
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 3,350 20 w/v -
  • Zinc acetate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Zinc acetate (1 M)
400.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium acetate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
533.3 μl of water (100 v/v)
C4
  • Polyethylene glycol 3,350 20 w/v -
  • Calcium acetate 0.2 M -
NA
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium acetate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Potassium acetate (5 M)
560.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium acetate 0.2 M -
NA
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium sulfate 0.2 M -
NA
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium sulfate 0.2 M -
NA
100.0 μl of Magnesium sulfate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium sulfate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
400.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium sulfate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium sulfate (0.5 M)
200.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
542.9 μl of water (100 v/v)
C12
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of di-Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
D1
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
D2
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium di-hydrogen phosphate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium di-hydrogen phosphate (4 M)
550.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium hydrogen phosphate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of di-Sodium hydrogen phosphate (1 M)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium di-hydrogen phosphate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium di-hydrogen phosphate (1.5 M)
466.7 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 20 w/v -
  • di-Potassium hydrogen phosphate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of di-Potassium hydrogen phosphate (4 M)
550.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
NA
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen phosphate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
542.9 μl of water (100 v/v)
D9
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Lithium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Lithium citrate (1.5 M)
466.7 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate (2.5 M)
520.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
520.0 μl of water (100 v/v)
E1
  • di-Sodium malonate 0.1 M pH 4
  • Polyethylene glycol 3,350 12 w/v -
pH 4
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 4 (3.4 M)
730.6 μl of water (100 v/v)
E2
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 4 (3.4 M)
541.2 μl of water (100 v/v)
E3
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 5 (3.4 M)
730.6 μl of water (100 v/v)
E4
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 5 (3.4 M)
541.2 μl of water (100 v/v)
E5
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 6 (3.4 M)
730.6 μl of water (100 v/v)
E6
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 6 (3.4 M)
541.2 μl of water (100 v/v)
E7
  • di-Sodium malonate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
29.4 μl of di-Sodium malonate pH 7 (3.4 M)
730.6 μl of water (100 v/v)
E8
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
541.2 μl of water (100 v/v)
E9
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
E10
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
E11
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
E12
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
F1
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
F2
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
F3
  • Tacsimate 12 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
120.0 μl of Tacsimate pH 7 (100 v/v)
640.0 μl of water (100 v/v)
F4
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
F5
  • Tacsimate 4 v/v -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Tacsimate pH 7 (100 v/v)
720.0 μl of water (100 v/v)
F6
  • Tacsimate 8 v/v -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Tacsimate pH 7 (100 v/v)
520.0 μl of water (100 v/v)
F7
  • di-Sodium succinate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
83.3 μl of di-Sodium succinate (1.2 M)
676.7 μl of water (100 v/v)
F8
  • di-Sodium succinate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
166.7 μl of di-Sodium succinate (1.2 M)
433.3 μl of water (100 v/v)
F9
  • tri-Ammonium citrate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of tri-Ammonium citrate (2.5 M)
720.0 μl of water (100 v/v)
F10
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
520.0 μl of water (100 v/v)
F11
  • di-Sodium DL-malate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of di-Sodium DL-malate (3 M)
726.7 μl of water (100 v/v)
F12
  • di-Sodium DL-malate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of di-Sodium DL-malate (3 M)
533.3 μl of water (100 v/v)
G1
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium acetate (4 M)
735.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate (4 M)
550.0 μl of water (100 v/v)
G3
  • Sodium formate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Sodium formate (5 M)
740.0 μl of water (100 v/v)
G4
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium formate (5 M)
560.0 μl of water (100 v/v)
G5
  • di-Ammonium tartrate 0.1 M -
  • Polyethylene glycol 3,350 12 w/v -
NA
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
62.5 μl of di-Ammonium tartrate (1.6 M)
697.5 μl of water (100 v/v)
G6
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of di-Ammonium tartrate (1.6 M)
475.0 μl of water (100 v/v)
G7
  • Tacsimate 2 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 3,350 16 w/v -
pH 4.6
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Tacsimate pH 7 (100 v/v)
560.0 μl of water (100 v/v)
G8
  • Tacsimate 2 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 3,350 16 w/v -
pH 5.6
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
560.0 μl of water (100 v/v)
G9
  • Tacsimate 2 v/v -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
480.0 μl of water (100 v/v)
G10
  • Tacsimate 2 v/v -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
480.0 μl of water (100 v/v)
G11
  • Tacsimate 2 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
20.0 μl of Tacsimate pH 7 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 3.4
pH 3.4
100.0 μl of CBTP buffer pH 2.2 (1 M)
0.0 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 4.1
pH 4.1
99.8 μl of CBTP buffer pH 2.2 (1 M)
0.2 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 5
pH 5
98.4 μl of CBTP buffer pH 2.2 (1 M)
1.6 μl of CBTP buffer pH 7.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 3,350 20 w/v -
  • CBTP buffer 0.1 M pH 6.4
pH 6.4
67.3 μl of CBTP buffer pH 2.2 (1 M)
32.7 μl of CBTP buffer pH 7.2 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 3,350 20 w/v -
  • CBTP buffer 0.1 M pH 7.6
pH 7.6
81.2 μl of CBTP buffer pH 8.8 (1 M)
18.8 μl of CBTP buffer pH 2.2 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 3,350 16 w/v -
  • CBTP buffer 0.1 M pH 8.8
pH 8.8
100.0 μl of CBTP buffer pH 8.8 (1 M)
-0.0 μl of CBTP buffer pH 2.2 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H6
  • Calcium chloride 0.02 M -
  • Cadmium chloride 0.02 M -
  • Cobalt (II) chloride 0.02 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Cadmium chloride (1 M)
10.0 μl of Calcium chloride (2 M)
20.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
H7
  • Magnesium chloride 0.01 M -
  • Nickel (II) chloride 0.005 M -
  • Polyethylene glycol 3,350 15 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
5.0 μl of Magnesium chloride (2 M)
50.0 μl of Nickel (II) chloride (0.1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
545.0 μl of water (100 v/v)
H8
  • Zinc chloride 0.02 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Zinc chloride (2 M)
590.0 μl of water (100 v/v)
H9
  • Cesium chloride 0.15 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
150.0 μl of Cesium chloride (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
H10
  • Sodium bromide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium bromide (5 M)
560.0 μl of water (100 v/v)
H11
  • Tryptone 1 w/v -
  • HEPES 0.05 M pH 7
  • Polyethylene glycol 3,350 12 w/v -
  • Sodium azide 1 mM -
pH 7
50.0 μl of HEPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Sodium azide (100 mM)
100.0 μl of Tryptone (10 w/v)
600.0 μl of water (100 v/v)
H12
  • Tryptone 1 w/v -
  • HEPES 0.05 M pH 7
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium azide 1 mM -
pH 7
50.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Sodium azide (100 mM)
100.0 μl of Tryptone (10 w/v)
440.0 μl of water (100 v/v)