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Supplemental Information

Screen Description

Cations

Qiagen / Cations
Created 2009-11-16.

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Cations

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Screen Structure

Cations

Well Count 96
Number of distinct ingredients 19
Internal diversity (score mean dist) 0.906

Screen Composition

2025-08-16T06:25:35.823364 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Cations
Buffer 136
Salt 122

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Cations Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium chloride 3.5 M -
pH 4.6
700.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
200.0 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium chloride 1.75 M -
pH 4.6
350.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
A3
  • Sodium acetate 0.1 M pH 4.6
  • Lithium chloride 4 M -
pH 4.6
400.0 μl of Lithium chloride (10 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.6
  • Lithium chloride 2 M -
pH 4.6
200.0 μl of Lithium chloride (10 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 1.5 M -
pH 4.6
600.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 0.75 M -
pH 4.6
300.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
600.0 μl of water (100 v/v)
A7
  • MES 0.1 M pH 6.5
  • Ammonium chloride 3.5 M -
pH 6.5
700.0 μl of Ammonium chloride (5 M)
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of water (100 v/v)
A8
  • MES 0.1 M pH 6.5
  • Ammonium chloride 1.75 M -
pH 6.5
350.0 μl of Ammonium chloride (5 M)
100.0 μl of MES pH 6.5 (1 M)
550.0 μl of water (100 v/v)
A9
  • MES 0.1 M pH 6.5
  • Lithium chloride 4 M -
pH 6.5
400.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
A10
  • MES 0.1 M pH 6.5
  • Lithium chloride 2 M -
pH 6.5
200.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
A11
  • MES 0.1 M pH 6.5
  • Magnesium chloride 1.5 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Magnesium chloride (2.5 M)
300.0 μl of water (100 v/v)
A12
  • MES 0.1 M pH 6.5
  • Magnesium chloride 0.75 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Magnesium chloride (2.5 M)
600.0 μl of water (100 v/v)
B1
  • HEPES 0.1 M pH 7.5
  • Ammonium chloride 3.5 M -
pH 7.5
700.0 μl of Ammonium chloride (5 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of water (100 v/v)
B2
  • HEPES 0.1 M pH 7.5
  • Ammonium chloride 1.75 M -
pH 7.5
350.0 μl of Ammonium chloride (5 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
550.0 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Lithium chloride 4 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Lithium chloride (10 M)
500.0 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7.5
  • Lithium chloride 2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Lithium chloride (10 M)
700.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 1.5 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
600.0 μl of Magnesium chloride (2.5 M)
300.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.75 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
300.0 μl of Magnesium chloride (2.5 M)
600.0 μl of water (100 v/v)
B7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium chloride 3.5 M -
pH 8.5
700.0 μl of Ammonium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
B8
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium chloride 1.75 M -
pH 8.5
350.0 μl of Ammonium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
B9
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium chloride 4 M -
pH 8.5
400.0 μl of Lithium chloride (10 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B10
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium chloride 2 M -
pH 8.5
200.0 μl of Lithium chloride (10 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
B11
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 1.5 M -
pH 8.5
600.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B12
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.75 M -
pH 8.5
300.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium acetate 4.5 M -
pH 4.6
562.5 μl of Ammonium acetate (8 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
337.5 μl of water (100 v/v)
C2
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium acetate 2.25 M -
pH 4.6
281.2 μl of Ammonium acetate (8 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
618.8 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 4.6
  • Lithium acetate 2.5 M -
pH 4.6
500.0 μl of Lithium acetate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C4
  • Sodium acetate 0.1 M pH 4.6
  • Lithium acetate 1.25 M -
pH 4.6
250.0 μl of Lithium acetate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
650.0 μl of water (100 v/v)
C5
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium acetate 2 M -
pH 4.6
588.2 μl of Magnesium acetate (3.4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
311.8 μl of water (100 v/v)
C6
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium acetate 1 M -
pH 4.6
294.1 μl of Magnesium acetate (3.4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
605.9 μl of water (100 v/v)
C7
  • MES 0.1 M pH 6.5
  • Ammonium acetate 4.5 M -
pH 6.5
562.5 μl of Ammonium acetate (8 M)
100.0 μl of MES pH 6.5 (1 M)
337.5 μl of water (100 v/v)
C8
  • MES 0.1 M pH 6.5
  • Ammonium acetate 2.25 M -
pH 6.5
281.2 μl of Ammonium acetate (8 M)
100.0 μl of MES pH 6.5 (1 M)
618.8 μl of water (100 v/v)
C9
  • MES 0.1 M pH 6.5
  • Lithium acetate 2.5 M -
pH 6.5
500.0 μl of Lithium acetate (5 M)
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of water (100 v/v)
C10
  • MES 0.1 M pH 6.5
  • Lithium acetate 1.25 M -
pH 6.5
250.0 μl of Lithium acetate (5 M)
100.0 μl of MES pH 6.5 (1 M)
650.0 μl of water (100 v/v)
C11
  • MES 0.1 M pH 6.5
  • Magnesium acetate 2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
588.2 μl of Magnesium acetate (3.4 M)
311.8 μl of water (100 v/v)
C12
  • MES 0.1 M pH 6.5
  • Magnesium acetate 1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
294.1 μl of Magnesium acetate (3.4 M)
605.9 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 4.5 M -
pH 7.5
562.5 μl of Ammonium acetate (8 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
337.5 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 2.25 M -
pH 7.5
281.2 μl of Ammonium acetate (8 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
618.8 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 7.5
  • Lithium acetate 2.5 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
500.0 μl of Lithium acetate (5 M)
400.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7.5
  • Lithium acetate 1.25 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
250.0 μl of Lithium acetate (5 M)
650.0 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • Magnesium acetate 2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
588.2 μl of Magnesium acetate (3.4 M)
311.8 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 7.5
  • Magnesium acetate 1 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
294.1 μl of Magnesium acetate (3.4 M)
605.9 μl of water (100 v/v)
D7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium acetate 4.5 M -
pH 8.5
562.5 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
337.5 μl of water (100 v/v)
D8
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium acetate 2.25 M -
pH 8.5
281.2 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
618.8 μl of water (100 v/v)
D9
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium acetate 2.5 M -
pH 8.5
500.0 μl of Lithium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D10
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium acetate 1.25 M -
pH 8.5
250.0 μl of Lithium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium acetate 2 M -
pH 8.5
588.2 μl of Magnesium acetate (3.4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
311.8 μl of water (100 v/v)
D12
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium acetate 1 M -
pH 8.5
294.1 μl of Magnesium acetate (3.4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
605.9 μl of water (100 v/v)
E1
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 2.2 M -
pH 4.6
880.0 μl of Calcium chloride (2.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of water (100 v/v)
E2
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 1.1 M -
pH 4.6
550.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
E3
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 3.2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
640.0 μl of Sodium chloride (5 M)
260.0 μl of water (100 v/v)
E4
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 1.6 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
320.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
E5
  • Sodium acetate 0.1 M pH 4.6
  • Zinc sulfate 1.6 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
800.0 μl of Zinc sulfate (2 M)
100.0 μl of water (100 v/v)
E6
  • Sodium acetate 0.1 M pH 4.6
  • Zinc sulfate 0.8 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Zinc sulfate (2 M)
500.0 μl of water (100 v/v)
E7
  • MES 0.1 M pH 6.5
  • Calcium chloride 2.2 M -
pH 6.5
880.0 μl of Calcium chloride (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
20.0 μl of water (100 v/v)
E8
  • MES 0.1 M pH 6.5
  • Calcium chloride 1.1 M -
pH 6.5
550.0 μl of Calcium chloride (2 M)
100.0 μl of MES pH 6.5 (1 M)
350.0 μl of water (100 v/v)
E9
  • MES 0.1 M pH 6.5
  • Sodium chloride 3.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
640.0 μl of Sodium chloride (5 M)
260.0 μl of water (100 v/v)
E10
  • MES 0.1 M pH 6.5
  • Sodium chloride 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
320.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
E11
  • Sodium cacodylate 0.1 M pH 6.5
  • Zinc sulfate 1.6 M -
pH 6.5
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
800.0 μl of Zinc sulfate (2 M)
100.0 μl of water (100 v/v)
E12
  • Sodium cacodylate 0.1 M pH 6.5
  • Zinc sulfate 0.8 M -
pH 6.5
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
400.0 μl of Zinc sulfate (2 M)
500.0 μl of water (100 v/v)
F1
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 2.2 M -
pH 7.5
880.0 μl of Calcium chloride (2.5 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
20.0 μl of water (100 v/v)
F2
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 1.1 M -
pH 7.5
550.0 μl of Calcium chloride (2 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
350.0 μl of water (100 v/v)
F3
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 3.2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
640.0 μl of Sodium chloride (5 M)
260.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 1.6 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
320.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
F5
  • Imidazole 0.1 M pH 7.5
  • Zinc sulfate 1.6 M -
pH 7.5
36.5 μl of Imidazole pH 7 (1 M)
63.5 μl of Imidazole pH 8 (1 M)
800.0 μl of Zinc sulfate (2 M)
100.0 μl of water (100 v/v)
F6
  • Imidazole 0.1 M pH 7.5
  • Zinc sulfate 0.8 M -
pH 7.5
36.5 μl of Imidazole pH 7 (1 M)
63.5 μl of Imidazole pH 8 (1 M)
400.0 μl of Zinc sulfate (2 M)
500.0 μl of water (100 v/v)
F7
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 2.2 M -
pH 8.5
880.0 μl of Calcium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
20.0 μl of water (100 v/v)
F8
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 1.1 M -
pH 8.5
550.0 μl of Calcium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
F9
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 3.2 M -
pH 8.5
640.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
260.0 μl of water (100 v/v)
F10
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 1.6 M -
pH 8.5
320.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
F11
  • BICINE 0.1 M pH 8.5
  • Zinc sulfate 1.6 M -
pH 8.5
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
800.0 μl of Zinc sulfate (2 M)
100.0 μl of water (100 v/v)
F12
  • BICINE 0.1 M pH 8.5
  • Zinc sulfate 0.8 M -
pH 8.5
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
400.0 μl of Zinc sulfate (2 M)
500.0 μl of water (100 v/v)
G1
  • Sodium acetate 0.1 M pH 4.6
  • Potassium acetate 4 M -
pH 4.6
800.0 μl of Potassium acetate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.1 M pH 4.6
  • Potassium acetate 2 M -
pH 4.6
400.0 μl of Potassium acetate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G3
  • Sodium acetate 0.1 M pH 4.6
  • Potassium chloride 2.2 M -
pH 4.6
550.0 μl of Potassium chloride (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
G4
  • Sodium acetate 0.1 M pH 4.6
  • Potassium chloride 1.1 M -
pH 4.6
275.0 μl of Potassium chloride (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
625.0 μl of water (100 v/v)
G5
  • Sodium acetate 0.1 M pH 4.6
  • Zinc acetate 1.2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
800.0 μl of Zinc acetate (1.5 M)
100.0 μl of water (100 v/v)
G6
  • Sodium acetate 0.1 M pH 4.6
  • Zinc acetate 0.6 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Zinc acetate (1.5 M)
500.0 μl of water (100 v/v)
G7
  • MES 0.1 M pH 6.5
  • Potassium acetate 4 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Potassium acetate (5 M)
100.0 μl of water (100 v/v)
G8
  • MES 0.1 M pH 6.5
  • Potassium acetate 2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Potassium acetate (5 M)
500.0 μl of water (100 v/v)
G9
  • MES 0.1 M pH 6.5
  • Potassium chloride 2.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
550.0 μl of Potassium chloride (4 M)
350.0 μl of water (100 v/v)
G10
  • MES 0.1 M pH 6.5
  • Potassium chloride 1.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
275.0 μl of Potassium chloride (4 M)
625.0 μl of water (100 v/v)
G11
  • MES 0.1 M pH 6.5
  • Zinc acetate 1.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Zinc acetate (1.5 M)
100.0 μl of water (100 v/v)
G12
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Zinc acetate (1.5 M)
500.0 μl of water (100 v/v)
H1
  • HEPES 0.1 M pH 7.5
  • Potassium acetate 4 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
800.0 μl of Potassium acetate (5 M)
100.0 μl of water (100 v/v)
H2
  • HEPES 0.1 M pH 7.5
  • Potassium acetate 2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Potassium acetate (5 M)
500.0 μl of water (100 v/v)
H3
  • HEPES 0.1 M pH 7.5
  • Potassium chloride 2.2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
550.0 μl of Potassium chloride (4 M)
350.0 μl of water (100 v/v)
H4
  • HEPES 0.1 M pH 7.5
  • Potassium chloride 1.1 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
275.0 μl of Potassium chloride (4 M)
625.0 μl of water (100 v/v)
H5
  • Imidazole 0.1 M pH 7.5
  • Zinc acetate 1.2 M -
pH 7.5
36.5 μl of Imidazole pH 7 (1 M)
63.5 μl of Imidazole pH 8 (1 M)
800.0 μl of Zinc acetate (1.5 M)
100.0 μl of water (100 v/v)
H6
  • Imidazole 0.1 M pH 7.5
  • Zinc acetate 0.6 M -
pH 7.5
36.5 μl of Imidazole pH 7 (1 M)
63.5 μl of Imidazole pH 8 (1 M)
400.0 μl of Zinc acetate (1.5 M)
500.0 μl of water (100 v/v)
H7
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium acetate 4 M -
pH 8.5
800.0 μl of Potassium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium acetate 2 M -
pH 8.5
400.0 μl of Potassium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H9
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium chloride 2.2 M -
pH 8.5
550.0 μl of Potassium chloride (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
H10
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium chloride 1.1 M -
pH 8.5
275.0 μl of Potassium chloride (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
H11
  • tris chloride BCC 0.1 M pH 8.5
  • Zinc acetate 1.2 M -
pH 8.5
800.0 μl of Zinc acetate (1.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8.5
  • Zinc acetate 0.6 M -
pH 8.5
400.0 μl of Zinc acetate (1.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)