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Supplemental Information

Screen Description

Classics Suite I

Qiagen / Classics Suite I
Created 2009-11-16.

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Classics Suite I

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Screen Structure

Classics Suite I

Well Count 96
Number of distinct ingredients 55
Internal diversity (score mean dist) 0.952

Screen Composition

2025-08-16T14:27:56.259230 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classics Suite I
Buffer 107
Cryoprotectant 1
Detergent 1
Organic (non-volatile) 24
Organic (volatile) 13
Polymer 41
Salt 116

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classics Suite I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.6
  • 1,6-Hexanediol 1 v/v -
  • Cobalt (II) chloride 0.01 M -
pH 4.6
14.1 μl of 1,6-Hexanediol (71 v/v)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
875.9 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.6
  • 1,6-Hexanediol 2.5 v/v -
pH 5.6
35.2 μl of 1,6-Hexanediol (71 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
864.8 μl of water (100 v/v)
A3
  • tris chloride BCC 0.1 M pH 8.5
  • 1,6-Hexanediol 3.4 v/v -
  • Magnesium chloride 0.2 M -
pH 8.5
47.9 μl of 1,6-Hexanediol (71 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
772.1 μl of water (100 v/v)
A4
  • 2-Propanol 5 v/v -
  • Ammonium sulfate 2 M -
NA
50.0 μl of 2-Propanol (100 v/v)
500.0 μl of Ammonium sulfate (4 M)
450.0 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 20 v/v -
  • Calcium chloride 0.2 M -
pH 4.6
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
600.0 μl of water (100 v/v)
A7
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
A8
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 20 v/v -
  • tri-Sodium citrate 0.2 M -
pH 7.5
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
575.0 μl of water (100 v/v)
A9
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 30 v/v -
  • tri-Sodium citrate 0.2 M -
pH 6.5
300.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A10
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 30 v/v -
  • Magnesium chloride 0.2 M -
pH 7.5
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
520.0 μl of water (100 v/v)
A11
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 30 v/v -
  • Ammonium acetate 0.2 M -
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
A12
  • Ethanol 10 v/v -
  • Sodium chloride 1.5 M -
NA
100.0 μl of Ethanol (100 v/v)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 20 v/v -
pH 8.5
200.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
B2
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Calcium chloride 0.02 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
B4
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium chloride 0.2 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Ammonium acetate 0.2 M -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
B6
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Magnesium acetate 0.2 M -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
58.8 μl of Magnesium acetate (3.4 M)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
541.2 μl of water (100 v/v)
B7
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • tri-Sodium citrate 0.2 M -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
B8
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Ammonium sulfate 0.5 M -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
125.0 μl of Ammonium sulfate (4 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
475.0 μl of water (100 v/v)
B9
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B10
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of water (100 v/v)
B11
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Methyl-2-propanol 25 v/v -
pH 8.5
250.0 μl of 2-Methyl-2-propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
B12
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Methyl-2-propanol 35 v/v -
pH 5.6
350.0 μl of 2-Methyl-2-propanol (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
550.0 μl of water (100 v/v)
C1
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
160.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
840.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
500.0 μl of water (100 v/v)
C3
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7.5
  • Ammonium formate 2 M -
pH 7.5
200.0 μl of Ammonium formate (10 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
700.0 μl of water (100 v/v)
C5
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C6
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C7
  • Ammonium sulfate 2 M -
NA
500.0 μl of Ammonium sulfate (4 M)
500.0 μl of water (100 v/v)
C8
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
  • Sodium chloride 0.1 M -
pH 7.5
400.0 μl of Ammonium sulfate (4 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
20.0 μl of Sodium chloride (5 M)
480.0 μl of water (100 v/v)
C9
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
  • Cobalt (II) chloride 0.01 M -
pH 6.5
450.0 μl of Ammonium sulfate (4 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
440.0 μl of water (100 v/v)
C10
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
  • Potassium Sodium tartrate 0.2 M -
pH 5.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
C11
  • Imidazole 1 M -
NA
1000.0 μl of Imidazole (1 M)
C12
  • Potassium Sodium tartrate 0.4 M -
NA
200.0 μl of Potassium Sodium tartrate (2 M)
800.0 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
D2
  • Imidazole 0.1 M pH 6.5
  • Sodium acetate 1 M -
pH 6.5
63.5 μl of Imidazole pH 6 (1 M)
36.5 μl of Imidazole pH 7 (1 M)
333.3 μl of Sodium acetate (3 M)
566.7 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1 M -
  • Cadmium sulfate 0.05 M -
pH 7.5
50.0 μl of Cadmium sulfate (1 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
333.3 μl of Sodium acetate (3 M)
516.7 μl of water (100 v/v)
D4
  • Sodium cacodylate 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
466.7 μl of Sodium acetate (3 M)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
433.3 μl of water (100 v/v)
D5
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
D6
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
  • Sodium di-hydrogen phosphate 0.1 M -
  • Potassium di-hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
25.0 μl of Potassium di-hydrogen phosphate (4 M)
400.0 μl of Sodium chloride (5 M)
33.3 μl of Sodium di-hydrogen phosphate (3 M)
441.7 μl of water (100 v/v)
D7
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4.3 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)
D8
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
D9
  • tri-Sodium citrate 1.6 M -
NA
1000.0 μl of tri-Sodium citrate (1.6 M)
D10
  • HEPES 0.1 M pH 7.5
  • Sodium di-hydrogen phosphate 0.8 M -
  • Potassium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
266.7 μl of Sodium di-hydrogen phosphate (3 M)
433.3 μl of water (100 v/v)
D11
  • Sodium acetate 0.1 M pH 4.6
  • Sodium formate 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
D12
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
E1
  • BICINE 0.1 M pH 9
  • 1,4-Dioxane 2 v/v -
  • Polyethylene glycol 20,000 10 w/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
333.3 μl of Polyethylene glycol 20,000 (30 w/v)
526.7 μl of water (100 v/v)
E2
  • MES 0.1 M pH 6.5
  • 1,4-Dioxane 10 v/v -
  • Ammonium sulfate 1.6 M -
pH 6.5
200.0 μl of 1,4-Dioxane (50 v/v)
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of water (100 v/v)
E3
  • 1,4-Dioxane 35 v/v -
NA
700.0 μl of 1,4-Dioxane (50 v/v)
300.0 μl of water (100 v/v)
E4
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ethylene imine polymer 2 v/v -
  • Sodium chloride 0.5 M -
pH 5.6
40.0 μl of Ethylene imine polymer (50 v/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
760.0 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 12 v/v -
  • Ammonium sulfate 1.5 M -
pH 8.5
375.0 μl of Ammonium sulfate (4 M)
150.0 μl of Glycerol (80 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
375.0 μl of water (100 v/v)
E6
  • Cetyltrimethylammonium bromide 0.01 mM -
  • Sodium chloride 0.5 M -
  • Magnesium chloride 0.01 M -
NA
0.2 μl of Cetyltrimethylammonium bromide (50 mM)
4.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium chloride (5 M)
895.8 μl of water (100 v/v)
E7
  • tri-Sodium citrate 0.1 M pH 5.6
  • Jeffamine® M-600 10 v/v -
  • Iron (III) chloride 0.01 M -
pH 5.6
10.0 μl of Iron (III) chloride (1 M)
200.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
E8
  • HEPES 0.1 M pH 7.5
  • Jeffamine® M-600 20 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Jeffamine® M-600 (50 v/v)
500.0 μl of water (100 v/v)
E9
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 1 M -
  • Ammonium sulfate 0.5 M -
pH 5.6
125.0 μl of Ammonium sulfate (4 M)
370.4 μl of Lithium sulfate (2.7 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
404.6 μl of water (100 v/v)
E10
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1 M -
  • Nickel (II) chloride 0.01 M -
pH 8.5
370.4 μl of Lithium sulfate (2.7 M)
2.5 μl of Nickel (II) chloride (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
527.1 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
555.6 μl of Lithium sulfate (2.7 M)
344.4 μl of water (100 v/v)
E12
  • BICINE 0.1 M pH 9
  • Magnesium chloride 2 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of water (100 v/v)
F1
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
800.0 μl of water (100 v/v)
F2
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
640.0 μl of Magnesium sulfate (2.5 M)
260.0 μl of water (100 v/v)
F3
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 8,000 15 w/v -
  • Lithium sulfate 0.5 M -
NA
185.2 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
514.8 μl of water (100 v/v)
F6
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
  • Zinc acetate 0.2 M -
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
133.3 μl of Zinc acetate (1.5 M)
406.7 μl of water (100 v/v)
F7
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
340.0 μl of water (100 v/v)
F8
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium acetate 0.2 M -
pH 6.5
58.8 μl of Magnesium acetate (3.4 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
441.2 μl of water (100 v/v)
F9
  • Polyethylene glycol 8,000 20 w/v -
  • Potassium di-hydrogen phosphate 0.05 M -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
12.5 μl of Potassium di-hydrogen phosphate (4 M)
587.5 μl of water (100 v/v)
F10
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
pH 6.5
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
250.0 μl of water (100 v/v)
F11
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium acetate 0.2 M -
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
233.3 μl of water (100 v/v)
F12
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
350.0 μl of water (100 v/v)
G1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
  • Ammonium sulfate 2 M -
pH 7.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
380.0 μl of water (100 v/v)
G2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
G3
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
  • Cadmium chloride 0.1 M -
pH 4.6
100.0 μl of Cadmium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
G5
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.2 M -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
G6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 18.3 v/v -
  • Sodium chloride 0.1 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
182.8 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
20.0 μl of Sodium chloride (5 M)
697.2 μl of water (100 v/v)
G7
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 22.9 v/v -
  • Zinc sulfate 0.01 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
228.5 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
5.0 μl of Zinc sulfate (2 M)
666.5 μl of water (100 v/v)
G8
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
G10
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Nickel (II) chloride 0.01 M -
pH 8.5
2.5 μl of Nickel (II) chloride (4 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
497.5 μl of water (100 v/v)
G11
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Ammonium sulfate 0.2 M -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
250.0 μl of water (100 v/v)
G12
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
H1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
H2
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
H3
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
100.0 μl of water (100 v/v)
H4
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
225.9 μl of water (100 v/v)
H6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
  • Sodium acetate 0.2 M -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
H7
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
350.0 μl of water (100 v/v)
H8
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • Ammonium sulfate 0.2 M -
pH 6.5
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
250.0 μl of water (100 v/v)
H9
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 6,000 10 w/v -
  • Sodium chloride 2 M -
NA
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
H11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
420.0 μl of water (100 v/v)
H12
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)