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Screen Description

Classics Suite I

Qiagen / Classics Suite I
Created 2009-11-16.

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Classics Suite I

    Screen Structure

    Classics Suite I

    Well Count 96
    Number of distinct ingredients 55
    Internal diversity (score mean dist) 0.952

    Screen Composition

    2025-06-13T21:40:05.752078 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classics Suite I
    Buffer 107
    Cryoprotectant 1
    Detergent 1
    Organic (non-volatile) 24
    Organic (volatile) 13
    Polymer 41
    Salt 116

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classics Suite I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.6
    • 1,6-Hexanediol 1 v/v -
    • Cobalt (II) chloride 0.01 M -
    pH 4.6
    10 μl of Cobalt (II) chloride (1 M)
    14 μl of 1,6-Hexanediol (71 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    875 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.5 v/v -
    pH 5.6
    35 μl of 1,6-Hexanediol (71 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    864 μl of water (100 v/v)
    A3
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 v/v -
    • Magnesium chloride 0.2 M -
    pH 8.5
    47 μl of 1,6-Hexanediol (71 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    772 μl of water (100 v/v)
    A4
    • 2-Propanol 5 v/v -
    • Ammonium sulfate 2 M -
    NA
    500 μl of Ammonium sulfate (4 M)
    50 μl of 2-Propanol (100 v/v)
    450 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 20 v/v -
    • Calcium chloride 0.2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Calcium chloride (2 M)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    A8
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 20 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    575 μl of water (100 v/v)
    A9
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 30 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 6.5
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    300 μl of 2-Propanol (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    A10
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 30 v/v -
    • Magnesium chloride 0.2 M -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    520 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 30 v/v -
    • Ammonium acetate 0.2 M -
    pH 8.5
    200 μl of Ammonium acetate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    A12
    • Ethanol 10 v/v -
    • Sodium chloride 1.5 M -
    NA
    100 μl of Ethanol (100 v/v)
    300 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    pH 8.5
    200 μl of Ethanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    B2
    • Ethylene glycol 25 v/v -
    NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    B3
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Calcium chloride 0.02 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Calcium chloride (2 M)
    590 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium chloride 0.2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    40 μl of Sodium chloride (5 M)
    560 μl of water (100 v/v)
    B5
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Ammonium acetate 0.2 M -
    pH 5.6
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    B6
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Magnesium acetate 0.2 M -
    pH 6.5
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    58 μl of Magnesium acetate (3.4 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    541 μl of water (100 v/v)
    B7
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    B8
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Ammonium sulfate 0.5 M -
    pH 7.5
    125 μl of Ammonium sulfate (4 M)
    38 μl of HEPES pH 6.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    475 μl of water (100 v/v)
    B9
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • Ammonium di-hydrogen phosphate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    320 μl of water (100 v/v)
    B10
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of water (100 v/v)
    B11
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Methyl-2-propanol 25 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of 2-Methyl-2-propanol (100 v/v)
    650 μl of water (100 v/v)
    B12
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Methyl-2-propanol 35 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    350 μl of 2-Methyl-2-propanol (100 v/v)
    550 μl of water (100 v/v)
    C1
    • Ammonium di-hydrogen phosphate 0.4 M -
    NA
    160 μl of Ammonium di-hydrogen phosphate (2.5 M)
    840 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 1 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of water (100 v/v)
    C3
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 2 M -
    pH 8.5
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7.5
    • Ammonium formate 2 M -
    pH 7.5
    200 μl of Ammonium formate (10 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    700 μl of water (100 v/v)
    C5
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    500 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    C6
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    500 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    C7
    • Ammonium sulfate 2 M -
    NA
    500 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    C8
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    • Sodium chloride 0.1 M -
    pH 7.5
    400 μl of Ammonium sulfate (4 M)
    38 μl of HEPES pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    61 μl of HEPES pH 8 (1 M)
    480 μl of water (100 v/v)
    C9
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    • Cobalt (II) chloride 0.01 M -
    pH 6.5
    10 μl of Cobalt (II) chloride (1 M)
    450 μl of Ammonium sulfate (4 M)
    100 μl of MES pH 6.5 (1 M)
    440 μl of water (100 v/v)
    C10
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    • Potassium Sodium tartrate 0.2 M -
    pH 5.6
    500 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of Potassium Sodium tartrate (2 M)
    300 μl of water (100 v/v)
    C11
    • Imidazole 1 M -
    NA
    1000 μl of Imidazole (1 M)
    C12
    • Potassium Sodium tartrate 0.4 M -
    NA
    200 μl of Potassium Sodium tartrate (2 M)
    800 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.8 M -
    pH 7.5
    400 μl of Potassium Sodium tartrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    D2
    • Imidazole 0.1 M pH 6.5
    • Sodium acetate 1 M -
    pH 6.5
    333 μl of Sodium acetate (3 M)
    63 μl of Imidazole pH 6 (1 M)
    36 μl of Imidazole pH 7 (1 M)
    566 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1 M -
    • Cadmium sulfate 0.05 M -
    pH 7.5
    333 μl of Sodium acetate (3 M)
    50 μl of Cadmium sulfate (1 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    516 μl of water (100 v/v)
    D4
    • Sodium cacodylate 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    466 μl of Sodium acetate (3 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    433 μl of water (100 v/v)
    D5
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    D6
    • MES 0.1 M pH 6.5
    • Sodium chloride 2 M -
    • Sodium di-hydrogen phosphate 0.1 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    pH 6.5
    25 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of MES pH 6.5 (1 M)
    400 μl of Sodium chloride (5 M)
    33 μl of Sodium di-hydrogen phosphate (3 M)
    441 μl of water (100 v/v)
    D7
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 4.3 M -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    860 μl of Sodium chloride (5 M)
    61 μl of HEPES pH 8 (1 M)
    40 μl of water (100 v/v)
    D8
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    875 μl of tri-Sodium citrate (1.6 M)
    25 μl of water (100 v/v)
    D9
    • tri-Sodium citrate 1.6 M -
    NA
    1000 μl of tri-Sodium citrate (1.6 M)
    D10
    • HEPES 0.1 M pH 7.5
    • Sodium di-hydrogen phosphate 0.8 M -
    • Potassium di-hydrogen phosphate 0.8 M -
    pH 7.5
    200 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    266 μl of Sodium di-hydrogen phosphate (3 M)
    433 μl of water (100 v/v)
    D11
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 2 M -
    pH 4.6
    285 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    D12
    • Sodium formate 4 M -
    NA
    571 μl of Sodium formate (7 M)
    428 μl of water (100 v/v)
    E1
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    • Polyethylene glycol 20,000 10 w/v -
    pH 9
    40 μl of 1,4-Dioxane (50 v/v)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of BICINE pH 9 (1 M)
    526 μl of water (100 v/v)
    E2
    • MES 0.1 M pH 6.5
    • 1,4-Dioxane 10 v/v -
    • Ammonium sulfate 1.6 M -
    pH 6.5
    200 μl of 1,4-Dioxane (50 v/v)
    400 μl of Ammonium sulfate (4 M)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    E3
    • 1,4-Dioxane 35 v/v -
    NA
    700 μl of 1,4-Dioxane (50 v/v)
    300 μl of water (100 v/v)
    E4
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ethylene imine polymer 2 v/v -
    • Sodium chloride 0.5 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Ethylene imine polymer (50 v/v)
    100 μl of Sodium chloride (5 M)
    760 μl of water (100 v/v)
    E5
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 12 v/v -
    • Ammonium sulfate 1.5 M -
    pH 8.5
    375 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Glycerol (80 v/v)
    375 μl of water (100 v/v)
    E6
    • Cetyltrimethylammonium bromide 0.01 mM -
    • Sodium chloride 0.5 M -
    • Magnesium chloride 0.01 M -
    NA
    0 μl of Cetyltrimethylammonium bromide (50 mM)
    4 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium chloride (5 M)
    895 μl of water (100 v/v)
    E7
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    • Iron (III) chloride 0.01 M -
    pH 5.6
    200 μl of Jeffamine® M-600 (50 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    10 μl of Iron (III) chloride (1 M)
    690 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    pH 7.5
    400 μl of Jeffamine® M-600 (50 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    500 μl of water (100 v/v)
    E9
    • tri-Sodium citrate 0.1 M pH 5.6
    • Lithium sulfate 1 M -
    • Ammonium sulfate 0.5 M -
    pH 5.6
    125 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    370 μl of Lithium sulfate (2.7 M)
    404 μl of water (100 v/v)
    E10
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    • Nickel (II) chloride 0.01 M -
    pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    370 μl of Lithium sulfate (2.7 M)
    527 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    pH 7.5
    555 μl of Lithium sulfate (2.7 M)
    100 μl of HEPES pH 7.5 (1 M)
    344 μl of water (100 v/v)
    E12
    • BICINE 0.1 M pH 9
    • Magnesium chloride 2 M -
    pH 9
    800 μl of Magnesium chloride (2.5 M)
    100 μl of BICINE pH 9 (1 M)
    100 μl of water (100 v/v)
    F1
    • Magnesium formate 0.2 M -
    NA
    200 μl of Magnesium formate (1 M)
    800 μl of water (100 v/v)
    F2
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    pH 6.5
    640 μl of Magnesium sulfate (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    260 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 8 w/v -
    pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    700 μl of water (100 v/v)
    F5
    • Polyethylene glycol 8,000 15 w/v -
    • Lithium sulfate 0.5 M -
    NA
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    185 μl of Lithium sulfate (2.7 M)
    514 μl of water (100 v/v)
    F6
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    • Zinc acetate 0.2 M -
    pH 6.5
    133 μl of Zinc acetate (1.5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    406 μl of water (100 v/v)
    F7
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    • Calcium acetate 0.2 M -
    pH 6.5
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    340 μl of water (100 v/v)
    F8
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    • Magnesium acetate 0.2 M -
    pH 6.5
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    58 μl of Magnesium acetate (3.4 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    441 μl of water (100 v/v)
    F9
    • Polyethylene glycol 8,000 20 w/v -
    • Potassium di-hydrogen phosphate 0.05 M -
    NA
    12 μl of Potassium di-hydrogen phosphate (4 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    587 μl of water (100 v/v)
    F10
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    pH 6.5
    50 μl of Ammonium sulfate (4 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    250 μl of water (100 v/v)
    F11
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    • Sodium acetate 0.2 M -
    pH 6.5
    66 μl of Sodium acetate (3 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    233 μl of water (100 v/v)
    F12
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    350 μl of water (100 v/v)
    G1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 v/v -
    • Ammonium sulfate 2 M -
    pH 7.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    380 μl of water (100 v/v)
    G2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 v/v -
    • Calcium chloride 0.2 M -
    pH 7.5
    280 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    G3
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.1 M -
    pH 4.6
    100 μl of Cadmium chloride (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    G4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    • Magnesium chloride 0.2 M -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    G6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 18.3 v/v -
    • Sodium chloride 0.1 M -
    pH 9
    182 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    20 μl of Sodium chloride (5 M)
    697 μl of water (100 v/v)
    G7
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 22.9 v/v -
    • Zinc sulfate 0.01 M -
    pH 6.5
    228 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    5 μl of Zinc sulfate (2 M)
    666 μl of water (100 v/v)
    G8
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    G9
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    G10
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • Nickel (II) chloride 0.01 M -
    pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    497 μl of water (100 v/v)
    G11
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    pH 4.6
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of water (100 v/v)
    G12
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    H1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    • Ammonium sulfate 0.2 M -
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    350 μl of water (100 v/v)
    H2
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium acetate 0.2 M -
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of water (100 v/v)
    H3
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium acetate 0.2 M -
    pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of water (100 v/v)
    H4
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    • Magnesium chloride 0.2 M -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    • Lithium sulfate 0.2 M -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    225 μl of water (100 v/v)
    H6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    • Sodium acetate 0.2 M -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    H7
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    350 μl of water (100 v/v)
    H8
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    pH 6.5
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    H9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 10 w/v -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    650 μl of water (100 v/v)
    H10
    • Polyethylene glycol 6,000 10 w/v -
    • Sodium chloride 2 M -
    NA
    400 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    H11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    • Ethylene glycol 8 v/v -
    pH 7.5
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    38 μl of HEPES pH 6.5 (1 M)
    80 μl of Ethylene glycol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    420 μl of water (100 v/v)
    H12
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)