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Supplemental Information

Screen Description

Qiagen / Classics Suite II
Created 2009-11-16.

Rigaku Recipe XML

μl
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Screen Structure

Well Count 96
Number of distinct ingredients 47
Internal diversity (Score mean dist)0.91
Internal diversity (Ion mean dist)0.89
Internal diversity (PEG mean dist)0.88
Internal diversity (Known pH mean dist)0.82
Internal diversity (Penalty mean dist)0.89

Screen Composition

Chemical classes bar chart
ingredient type Classics Suite II
Buffer 113
Organic (non-volatile) 8
Polymer 58
Salt 95

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classics Suite II Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Ammonium sulfate 2 M -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
400.0 μl of water (100 v/v)
A2
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
500.0 μl of Ammonium sulfate (4 M)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
400.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
400.0 μl of water (100 v/v)
A4
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of water (100 v/v)
A5
  • Ammonium sulfate 2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
500.0 μl of Ammonium sulfate (4 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
400.0 μl of water (100 v/v)
A6
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
A7
  • Sodium chloride 3 M -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
300.0 μl of water (100 v/v)
A8
  • Sodium chloride 3 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A9
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A10
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A11
  • Sodium chloride 3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A12
  • Sodium chloride 3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • Magnesium formate 0.3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Magnesium formate (1 M)
600.0 μl of water (100 v/v)
B2
  • Magnesium formate 0.5 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Magnesium formate (1 M)
400.0 μl of water (100 v/v)
B3
  • Magnesium formate 0.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Magnesium formate (1 M)
400.0 μl of water (100 v/v)
B4
  • Magnesium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of Magnesium formate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
B5
  • Sodium di-hydrogen phosphate 1.26 M -
  • Potassium di-hydrogen phosphate 0.14 M -
NA
35.0 μl of Potassium di-hydrogen phosphate (4 M)
420.0 μl of Sodium di-hydrogen phosphate (3 M)
545.0 μl of water (100 v/v)
B6
  • Sodium di-hydrogen phosphate 0.49 M -
  • Potassium di-hydrogen phosphate 0.91 M -
NA
227.5 μl of Potassium di-hydrogen phosphate (4 M)
163.33 μl of Sodium di-hydrogen phosphate (3 M)
609.17 μl of water (100 v/v)
B7
  • Sodium di-hydrogen phosphate 0.056 M -
  • Potassium di-hydrogen phosphate 1.33 M -
NA
333.5 μl of Potassium di-hydrogen phosphate (4 M)
18.67 μl of Sodium di-hydrogen phosphate (3 M)
647.83 μl of water (100 v/v)
B8
  • tri-Sodium citrate 1.4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
700.0 μl of tri-Sodium citrate pH 7 (2 M)
200.0 μl of water (100 v/v)
B9
  • tri-Ammonium citrate 1.8 M -
NA
600.0 μl of tri-Ammonium citrate pH 7 (3 M)
400.0 μl of water (100 v/v)
B10
  • di-Sodium succinate 0.8 M -
NA
800.0 μl of di-Sodium succinate pH 7 (1 M)
200.0 μl of water (100 v/v)
B11
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate pH 7 (3 M)
300.0 μl of water (100 v/v)
B12
  • Sodium acetate 2.8 M -
NA
933.33 μl of Sodium acetate pH 7 (3 M)
66.67 μl of water (100 v/v)
C1
  • Sodium formate 3.5 M -
NA
500.0 μl of Sodium formate pH 7 (7 M)
500.0 μl of water (100 v/v)
C2
  • di-Ammonium tartrate 1.1 M -
NA
687.5 μl of di-Ammonium tartrate pH 7 (1.6 M)
312.5 μl of water (100 v/v)
C3
  • di-Sodium malonate 2.4 M -
NA
705.88 μl of di-Sodium malonate pH 7 (3.4 M)
294.12 μl of water (100 v/v)
C4
  • tri-Sodium citrate 0.56 M -
NA
280.0 μl of tri-Sodium citrate pH 7 (2 M)
720.0 μl of water (100 v/v)
C5
  • tri-Sodium citrate 0.96 M -
NA
480.0 μl of tri-Sodium citrate pH 7 (2 M)
520.0 μl of water (100 v/v)
C6
  • Ammonium sulfate 1.5 M -
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
375.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
20.0 μl of Sodium chloride (5 M)
505.0 μl of water (100 v/v)
C7
  • Potassium Sodium tartrate 0.8 M -
  • Polyethylene glycol monomethyl ether 5,000 0.5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
10.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
400.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
C8
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 3,350 1 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
250.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
630.0 μl of water (100 v/v)
C9
  • di-Sodium malonate 1.1 M -
  • Jeffamine® ED-2001 0.5 v/v -
  • HEPES 0.1 M pH 7
pH 7
323.53 μl of di-Sodium malonate pH 7 (3.4 M)
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
10.0 μl of Jeffamine® ED-2001 (50 v/v)
566.47 μl of water (100 v/v)
C10
  • di-Sodium succinate 1 M -
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
  • HEPES 0.1 M pH 7
pH 7
833.33 μl of di-Sodium succinate (1.2 M)
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
46.67 μl of water (100 v/v)
C11
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 8,000 0.5 w/v -
  • HEPES 0.1 M pH 7
pH 7
250.0 μl of Ammonium sulfate (4 M)
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
10.0 μl of Polyethylene glycol 8,000 (50 w/v)
640.0 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.191 M -
  • Polyethylene glycol 3,350 2 w/v -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
40.0 μl of Polyethylene glycol 3,350 (50 w/v)
119.38 μl of tri-Sodium citrate (1.6 M)
740.62 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 25 w/v -
NA
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
D2
  • Jeffamine® M-600 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
300.0 μl of water (100 v/v)
D3
  • Jeffamine® ED-2001 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® ED-2001 (50 v/v)
300.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 25 w/v -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
400.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 25 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 3,350 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D10
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
D11
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
D12
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
E1
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
E2
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
E3
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
250.0 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
250.0 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
E6
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Calcium chloride 0.05 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
E7
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Magnesium chloride 0.05 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
20.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
580.0 μl of water (100 v/v)
E8
  • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
  • Potassium chloride 0.2 M -
  • HEPES 0.05 M pH 7.5
pH 7.5
19.42 μl of HEPES pH 6.5 (1 M)
30.58 μl of HEPES pH 8 (1 M)
350.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
E9
  • Pentaerythritol ethoxylate (3/4 EO/OH) 30 v/v -
  • Ammonium sulfate 0.05 M -
  • BIS-TRIS 0.05 M pH 6.5
pH 6.5
12.5 μl of Ammonium sulfate (4 M)
50.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (50 v/v)
337.5 μl of water (100 v/v)
E10
  • Polypropylene glycol 400 45 v/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
450.0 μl of Polypropylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
E11
  • Poly(acrylic acid sodium salt) 5,100 22 v/v -
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v)
452.0 μl of water (100 v/v)
E12
  • Polyvinylpyrrolidone K15 20 w/v -
  • Cobalt (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
10.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 10 w/v -
  • L-Proline 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
200.0 μl of L-Proline (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F2
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F3
  • Polyethylene glycol monomethyl ether 5,000 10 w/v -
  • tri-Sodium citrate 0.064 M -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
40.0 μl of tri-Sodium citrate (1.6 M)
660.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 3,350 12 w/v -
  • Cobalt (II) chloride 0.005 M -
  • Cadmium chloride 0.005 M -
  • Nickel (II) chloride 0.005 M -
  • Magnesium chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
5.0 μl of Cadmium chloride (1 M)
5.0 μl of Cobalt (II) chloride (1 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
2.0 μl of Magnesium chloride (2.5 M)
1.25 μl of Nickel (II) chloride (4 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
646.75 μl of water (100 v/v)
F5
  • Polyethylene glycol 10,000 17 w/v -
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
50.0 μl of Ammonium sulfate (4 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of Ammonium sulfate (4 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
325.93 μl of water (100 v/v)
G3
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
325.93 μl of water (100 v/v)
G4
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
325.93 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
325.93 μl of water (100 v/v)
G6
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Ammonium acetate (1 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Ammonium acetate (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.24 M pH 7
pH 7
70.59 μl of di-Sodium malonate pH 7 (3.4 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
529.41 μl of water (100 v/v)
H4
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Ammonium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.67 μl of tri-Ammonium citrate pH 7 (3 M)
533.33 μl of water (100 v/v)
H5
  • Polyethylene glycol 3,350 15 w/v -
  • di-Sodium succinate 0.1 M pH 7
pH 7
100.0 μl of di-Sodium succinate pH 7 (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.57 μl of Sodium formate (7 M)
571.43 μl of water (100 v/v)
H7
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium DL-malate 0.15 M pH 7
pH 7
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 3,350 15 w/v -
  • Magnesium formate 0.1 M -
NA
100.0 μl of Magnesium formate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 3,350 20 w/v -
  • Zinc acetate 0.05 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.33 μl of Zinc acetate (1.5 M)
566.67 μl of water (100 v/v)
H10
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
H11
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium thiocyanate 0.1 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
H12
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)