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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Classics Suite II
Barcodes
Classics Suite II
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Screen Structure
Classics Suite II
Well Count | 96 |
---|---|
Number of distinct ingredients | 47 |
Internal diversity (score mean dist) | 0.906 |
Screen Composition
ingredient type | Classics Suite II |
---|---|
Buffer | 113 |
Organic (non-volatile) | 8 |
Polymer | 58 |
Salt | 95 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 400.0 μl of water (100 v/v) |
A2 |
| pH 4.5 | 500.0 μl of Ammonium sulfate (4 M) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 400.0 μl of water (100 v/v) |
A3 |
| pH 5.5 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 400.0 μl of water (100 v/v) |
A4 |
| pH 6.5 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of water (100 v/v) |
A5 |
| pH 7.5 | 500.0 μl of Ammonium sulfate (4 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 400.0 μl of water (100 v/v) |
A6 |
| pH 8.5 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
A7 |
| pH 3.5 | 600.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 300.0 μl of water (100 v/v) |
A8 |
| pH 4.5 | 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
A9 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
A10 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
A11 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
A12 |
| pH 8.5 | 600.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
B1 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Magnesium formate (1 M) 600.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Magnesium formate (1 M) 400.0 μl of water (100 v/v) |
B3 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Magnesium formate (1 M) 400.0 μl of water (100 v/v) |
B4 |
| pH 8.5 | 300.0 μl of Magnesium formate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
B5 |
| NA | 35.0 μl of Potassium di-hydrogen phosphate (4 M) 420.0 μl of Sodium di-hydrogen phosphate (3 M) 545.0 μl of water (100 v/v) |
B6 |
| NA | 227.5 μl of Potassium di-hydrogen phosphate (4 M) 163.3 μl of Sodium di-hydrogen phosphate (3 M) 609.2 μl of water (100 v/v) |
B7 |
| NA | 333.5 μl of Potassium di-hydrogen phosphate (4 M) 18.7 μl of Sodium di-hydrogen phosphate (3 M) 647.8 μl of water (100 v/v) |
B8 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 700.0 μl of tri-Sodium citrate pH 7 (2 M) 200.0 μl of water (100 v/v) |
B9 |
| NA | 600.0 μl of tri-Ammonium citrate pH 7 (3 M) 400.0 μl of water (100 v/v) |
B10 |
| NA | 800.0 μl of di-Sodium succinate pH 7 (1 M) 200.0 μl of water (100 v/v) |
B11 |
| NA | 700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v) |
B12 |
| NA | 933.3 μl of Sodium acetate pH 7 (3 M) 66.7 μl of water (100 v/v) |
C1 |
| NA | 500.0 μl of Sodium formate pH 7 (7 M) 500.0 μl of water (100 v/v) |
C2 |
| NA | 687.5 μl of di-Ammonium tartrate pH 7 (1.6 M) 312.5 μl of water (100 v/v) |
C3 |
| NA | 705.9 μl of di-Sodium malonate pH 7 (3.4 M) 294.1 μl of water (100 v/v) |
C4 |
| NA | 280.0 μl of tri-Sodium citrate pH 7 (2 M) 720.0 μl of water (100 v/v) |
C5 |
| NA | 480.0 μl of tri-Sodium citrate pH 7 (2 M) 520.0 μl of water (100 v/v) |
C6 |
| pH 6.5 | 375.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 20.0 μl of Sodium chloride (5 M) 505.0 μl of water (100 v/v) |
C7 |
| pH 8.5 | 10.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 400.0 μl of Potassium Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v) |
C8 |
| pH 5.5 | 250.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 20.0 μl of Polyethylene glycol 3,350 (50 w/v) 630.0 μl of water (100 v/v) |
C9 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 10.0 μl of Jeffamine® ED-2001 (50 v/v) 323.5 μl of di-Sodium malonate pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
C10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 833.3 μl of di-Sodium succinate (1.2 M) 46.7 μl of water (100 v/v) |
C11 |
| pH 7 | 250.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 10.0 μl of Polyethylene glycol 8,000 (50 w/v) 640.0 μl of water (100 v/v) |
C12 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 40.0 μl of Polyethylene glycol 3,350 (50 w/v) 119.4 μl of tri-Sodium citrate (1.6 M) 740.6 μl of water (100 v/v) |
D1 |
| NA | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
D2 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 300.0 μl of water (100 v/v) |
D3 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Jeffamine® ED-2001 (50 v/v) 300.0 μl of water (100 v/v) |
D4 |
| pH 3.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 400.0 μl of water (100 v/v) |
D5 |
| pH 4.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 400.0 μl of water (100 v/v) |
D6 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
D8 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
D9 |
| pH 8.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v) |
D11 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 340.0 μl of water (100 v/v) |
D12 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Calcium chloride (2 M) 350.0 μl of water (100 v/v) |
E1 |
| pH 6.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Calcium chloride (2 M) 350.0 μl of water (100 v/v) |
E2 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 250.0 μl of water (100 v/v) |
E3 |
| pH 6.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 250.0 μl of water (100 v/v) |
E4 |
| pH 7.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 250.0 μl of water (100 v/v) |
E5 |
| pH 8.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 25.0 μl of Calcium chloride (2 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 575.0 μl of water (100 v/v) |
E7 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 20.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 580.0 μl of water (100 v/v) |
E8 |
| pH 7.5 | 19.4 μl of HEPES pH 6.5 (1 M) 30.6 μl of HEPES pH 8 (1 M) 350.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v) 50.0 μl of Potassium chloride (4 M) 550.0 μl of water (100 v/v) |
E9 |
| pH 6.5 | 12.5 μl of Ammonium sulfate (4 M) 50.0 μl of BIS-TRIS pH 6.5 (1 M) 600.0 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (50 v/v) 337.5 μl of water (100 v/v) |
E10 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 450.0 μl of Polypropylene glycol 400 (100 v/v) 450.0 μl of water (100 v/v) |
E11 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v) 452.0 μl of water (100 v/v) |
E12 |
| pH 8.5 | 10.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v) |
F1 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 200.0 μl of L-Proline (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F2 |
| pH 8.5 | 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F3 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 40.0 μl of tri-Sodium citrate (1.6 M) 660.0 μl of water (100 v/v) |
F4 |
| pH 7.5 | 5.0 μl of Cadmium chloride (1 M) 5.0 μl of Cobalt (II) chloride (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 2.0 μl of Magnesium chloride (2.5 M) 1.2 μl of Nickel (II) chloride (4 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 646.8 μl of water (100 v/v) |
F5 |
| pH 5.5 | 100.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 460.0 μl of water (100 v/v) |
F6 |
| pH 5.5 | 50.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
F7 |
| pH 6.5 | 50.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
F8 |
| pH 7.5 | 50.0 μl of Ammonium sulfate (4 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
F9 |
| pH 8.5 | 50.0 μl of Ammonium sulfate (4 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
F10 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
F11 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
F12 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
G1 |
| pH 8.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 360.0 μl of water (100 v/v) |
G2 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 325.9 μl of water (100 v/v) |
G3 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 325.9 μl of water (100 v/v) |
G4 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 325.9 μl of water (100 v/v) |
G5 |
| pH 8.5 | 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 325.9 μl of water (100 v/v) |
G6 |
| pH 5.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
G7 |
| pH 6.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
G8 |
| pH 7.5 | 200.0 μl of Ammonium acetate (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
G9 |
| pH 8.5 | 200.0 μl of Ammonium acetate (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
G10 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
G11 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
G12 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
H1 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
H2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Potassium Sodium tartrate (2 M) 500.0 μl of water (100 v/v) |
H3 |
| pH 7 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 70.6 μl of di-Sodium malonate pH 7 (3.4 M) 529.4 μl of water (100 v/v) |
H4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of tri-Ammonium citrate pH 7 (3 M) 533.3 μl of water (100 v/v) |
H5 |
| pH 7 | 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Sodium succinate pH 7 (1 M) 600.0 μl of water (100 v/v) |
H6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium formate (7 M) 571.4 μl of water (100 v/v) |
H7 |
| pH 7 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of di-Sodium DL-malate pH 7 (3 M) 550.0 μl of water (100 v/v) |
H8 |
| NA | 100.0 μl of Magnesium formate (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 600.0 μl of water (100 v/v) |
H9 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of Zinc acetate (1.5 M) 566.7 μl of water (100 v/v) |
H10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 125.0 μl of tri-Sodium citrate (1.6 M) 475.0 μl of water (100 v/v) |
H11 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v) |
H12 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v) |