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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Classics Suite II
Barcodes
Classics Suite II
Screen Structure
Classics Suite II
Well Count | 96 |
---|---|
Number of distinct ingredients | 47 |
Internal diversity (score mean dist) | 0.906 |
Screen Composition
ingredient type | Classics Suite II |
---|---|
Buffer | 113 |
Organic (non-volatile) | 8 |
Polymer | 58 |
Salt | 95 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 500 μl of Ammonium sulfate (4 M) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 400 μl of water (100 v/v) |
A2 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 500 μl of Ammonium sulfate (4 M) 41 μl of Sodium acetate pH 5 (1 M) 400 μl of water (100 v/v) |
A3 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Ammonium sulfate (4 M) 400 μl of water (100 v/v) |
A4 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Ammonium sulfate (4 M) 400 μl of water (100 v/v) |
A5 |
| pH 7.5 | 61 μl of HEPES pH 8 (1 M) 500 μl of Ammonium sulfate (4 M) 38 μl of HEPES pH 6.5 (1 M) 400 μl of water (100 v/v) |
A6 |
| pH 8.5 | 500 μl of Ammonium sulfate (4 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
A7 |
| pH 3.5 | 600 μl of Sodium chloride (5 M) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 300 μl of water (100 v/v) |
A8 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 600 μl of Sodium chloride (5 M) 41 μl of Sodium acetate pH 5 (1 M) 300 μl of water (100 v/v) |
A9 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 600 μl of Sodium chloride (5 M) 300 μl of water (100 v/v) |
A10 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 600 μl of Sodium chloride (5 M) 300 μl of water (100 v/v) |
A11 |
| pH 7.5 | 61 μl of HEPES pH 8 (1 M) 600 μl of Sodium chloride (5 M) 38 μl of HEPES pH 6.5 (1 M) 300 μl of water (100 v/v) |
A12 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Sodium chloride (5 M) 300 μl of water (100 v/v) |
B1 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of Magnesium formate (1 M) 600 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Magnesium formate (1 M) 400 μl of water (100 v/v) |
B3 |
| pH 7.5 | 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 500 μl of Magnesium formate (1 M) 400 μl of water (100 v/v) |
B4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of Magnesium formate (1 M) 600 μl of water (100 v/v) |
B5 |
| NA | 420 μl of Sodium di-hydrogen phosphate (3 M) 35 μl of Potassium di-hydrogen phosphate (4 M) 545 μl of water (100 v/v) |
B6 |
| NA | 163 μl of Sodium di-hydrogen phosphate (3 M) 227 μl of Potassium di-hydrogen phosphate (4 M) 609 μl of water (100 v/v) |
B7 |
| NA | 18 μl of Sodium di-hydrogen phosphate (3 M) 333 μl of Potassium di-hydrogen phosphate (4 M) 647 μl of water (100 v/v) |
B8 |
| pH 7.5 | 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 700 μl of tri-Sodium citrate pH 7 (2 M) 200 μl of water (100 v/v) |
B9 |
| NA | 600 μl of tri-Ammonium citrate pH 7 (3 M) 400 μl of water (100 v/v) |
B10 |
| NA | 800 μl of di-Sodium succinate pH 7 (1 M) 200 μl of water (100 v/v) |
B11 |
| NA | 700 μl of di-Sodium DL-malate pH 7 (3 M) 300 μl of water (100 v/v) |
B12 |
| NA | 933 μl of Sodium acetate pH 7 (3 M) 66 μl of water (100 v/v) |
C1 |
| NA | 500 μl of Sodium formate pH 7 (7 M) 500 μl of water (100 v/v) |
C2 |
| NA | 687 μl of di-Ammonium tartrate pH 7 (1.6 M) 312 μl of water (100 v/v) |
C3 |
| NA | 705 μl of di-Sodium malonate pH 7 (3.4 M) 294 μl of water (100 v/v) |
C4 |
| NA | 280 μl of tri-Sodium citrate pH 7 (2 M) 720 μl of water (100 v/v) |
C5 |
| NA | 480 μl of tri-Sodium citrate pH 7 (2 M) 520 μl of water (100 v/v) |
C6 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 375 μl of Ammonium sulfate (4 M) 20 μl of Sodium chloride (5 M) 505 μl of water (100 v/v) |
C7 |
| pH 8.5 | 10 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Potassium Sodium tartrate (2 M) 490 μl of water (100 v/v) |
C8 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 20 μl of Polyethylene glycol 3,350 (50 w/v) 250 μl of Ammonium sulfate (4 M) 630 μl of water (100 v/v) |
C9 |
| pH 7 | 10 μl of Jeffamine® ED-2001 (50 v/v) 323 μl of di-Sodium malonate pH 7 (3.4 M) 22 μl of HEPES pH 8 (1 M) 77 μl of HEPES pH 6.5 (1 M) 566 μl of water (100 v/v) |
C10 |
| pH 7 | 20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 22 μl of HEPES pH 8 (1 M) 833 μl of di-Sodium succinate (1.2 M) 77 μl of HEPES pH 6.5 (1 M) 46 μl of water (100 v/v) |
C11 |
| pH 7 | 22 μl of HEPES pH 8 (1 M) 250 μl of Ammonium sulfate (4 M) 77 μl of HEPES pH 6.5 (1 M) 10 μl of Polyethylene glycol 8,000 (50 w/v) 640 μl of water (100 v/v) |
C12 |
| pH 7 | 40 μl of Polyethylene glycol 3,350 (50 w/v) 22 μl of HEPES pH 8 (1 M) 119 μl of tri-Sodium citrate (1.6 M) 77 μl of HEPES pH 6.5 (1 M) 740 μl of water (100 v/v) |
D1 |
| NA | 500 μl of Polyethylene glycol 1,500 (50 w/v) 500 μl of water (100 v/v) |
D2 |
| pH 7 | 600 μl of Jeffamine® M-600 (50 v/v) 22 μl of HEPES pH 8 (1 M) 77 μl of HEPES pH 6.5 (1 M) 300 μl of water (100 v/v) |
D3 |
| pH 7 | 600 μl of Jeffamine® ED-2001 (50 v/v) 22 μl of HEPES pH 8 (1 M) 77 μl of HEPES pH 6.5 (1 M) 300 μl of water (100 v/v) |
D4 |
| pH 3.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 400 μl of water (100 v/v) |
D5 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 41 μl of Sodium acetate pH 5 (1 M) 400 μl of water (100 v/v) |
D6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
D8 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 400 μl of water (100 v/v) |
D9 |
| pH 8.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500 μl of water (100 v/v) |
D11 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 340 μl of water (100 v/v) |
D12 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Calcium chloride (2 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 350 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 100 μl of Calcium chloride (2 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 350 μl of water (100 v/v) |
E2 |
| pH 5.5 | 200 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
E3 |
| pH 6.5 | 200 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
E4 |
| pH 7.5 | 200 μl of Ammonium acetate (1 M) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
E5 |
| pH 8.5 | 200 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
E6 |
| pH 6.5 | 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 25 μl of Calcium chloride (2 M) 575 μl of water (100 v/v) |
E7 |
| pH 7.5 | 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 20 μl of Magnesium chloride (2.5 M) 580 μl of water (100 v/v) |
E8 |
| pH 7.5 | 350 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v) 30 μl of HEPES pH 8 (1 M) 50 μl of Potassium chloride (4 M) 19 μl of HEPES pH 6.5 (1 M) 550 μl of water (100 v/v) |
E9 |
| pH 6.5 | 50 μl of BIS-TRIS pH 6.5 (1 M) 600 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (50 v/v) 12 μl of Ammonium sulfate (4 M) 337 μl of water (100 v/v) |
E10 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 450 μl of Polypropylene glycol 400 (100 v/v) 450 μl of water (100 v/v) |
E11 |
| pH 7.5 | 61 μl of HEPES pH 8 (1 M) 440 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v) 38 μl of HEPES pH 6.5 (1 M) 8 μl of Magnesium chloride (2.5 M) 452 μl of water (100 v/v) |
E12 |
| pH 8.5 | 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 10 μl of Cobalt (II) chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 490 μl of water (100 v/v) |
F1 |
| pH 7.5 | 200 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 200 μl of L-Proline (1 M) 500 μl of water (100 v/v) |
F2 |
| pH 8.5 | 200 μl of Trimethylamine N-oxide (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
F3 |
| pH 7 | 200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 22 μl of HEPES pH 8 (1 M) 40 μl of tri-Sodium citrate (1.6 M) 77 μl of HEPES pH 6.5 (1 M) 660 μl of water (100 v/v) |
F4 |
| pH 7.5 | 5 μl of Cadmium chloride (1 M) 1 μl of Nickel (II) chloride (4 M) 240 μl of Polyethylene glycol 3,350 (50 w/v) 5 μl of Cobalt (II) chloride (1 M) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 2 μl of Magnesium chloride (2.5 M) 646 μl of water (100 v/v) |
F5 |
| pH 5.5 | 100 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 460 μl of water (100 v/v) |
F6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Ammonium sulfate (4 M) 350 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Ammonium sulfate (4 M) 350 μl of water (100 v/v) |
F8 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 50 μl of Ammonium sulfate (4 M) 38 μl of HEPES pH 6.5 (1 M) 350 μl of water (100 v/v) |
F9 |
| pH 8.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Ammonium sulfate (4 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 350 μl of water (100 v/v) |
F10 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
F11 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
F12 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 40 μl of Sodium chloride (5 M) 38 μl of HEPES pH 6.5 (1 M) 360 μl of water (100 v/v) |
G1 |
| pH 8.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
G2 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 74 μl of Lithium sulfate (2.7 M) 325 μl of water (100 v/v) |
G3 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 74 μl of Lithium sulfate (2.7 M) 325 μl of water (100 v/v) |
G4 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 74 μl of Lithium sulfate (2.7 M) 325 μl of water (100 v/v) |
G5 |
| pH 8.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 74 μl of Lithium sulfate (2.7 M) 325 μl of water (100 v/v) |
G6 |
| pH 5.5 | 200 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of water (100 v/v) |
G7 |
| pH 6.5 | 200 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of water (100 v/v) |
G8 |
| pH 7.5 | 200 μl of Ammonium acetate (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 200 μl of water (100 v/v) |
G9 |
| pH 8.5 | 200 μl of Ammonium acetate (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of water (100 v/v) |
G10 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
G11 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
G12 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 61 μl of HEPES pH 8 (1 M) 38 μl of HEPES pH 6.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
H1 |
| pH 8.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
H2 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Potassium Sodium tartrate (2 M) 500 μl of water (100 v/v) |
H3 |
| pH 7 | 400 μl of Polyethylene glycol 3,350 (50 w/v) 70 μl of di-Sodium malonate pH 7 (3.4 M) 529 μl of water (100 v/v) |
H4 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of tri-Ammonium citrate pH 7 (3 M) 533 μl of water (100 v/v) |
H5 |
| pH 7 | 300 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of di-Sodium succinate pH 7 (1 M) 600 μl of water (100 v/v) |
H6 |
| NA | 28 μl of Sodium formate (7 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 571 μl of water (100 v/v) |
H7 |
| pH 7 | 50 μl of di-Sodium DL-malate pH 7 (3 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 550 μl of water (100 v/v) |
H8 |
| NA | 300 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Magnesium formate (1 M) 600 μl of water (100 v/v) |
H9 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 33 μl of Zinc acetate (1.5 M) 566 μl of water (100 v/v) |
H10 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 125 μl of tri-Sodium citrate (1.6 M) 475 μl of water (100 v/v) |
H11 |
| NA | 12 μl of Potassium thiocyanate (8 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 387 μl of water (100 v/v) |
H12 |
| NA | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37 μl of Potassium bromide (4 M) 362 μl of water (100 v/v) |