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Supplemental Information

Screen Description

JCSG Core I

Qiagen / JCSG Core I
Created 2009-11-16.

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Barcodes

JCSG Core I

No barcodes found.

Screen Structure

JCSG Core I

Well Count 96
Number of distinct ingredients 65
Internal diversity (score mean dist) 0.924

Screen Composition

2025-08-16T04:27:16.891382 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG Core I
Buffer 83
Cryoprotectant 2
Organic (non-volatile) 17
Organic (volatile) 5
Polymer 81
Salt 101

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Core I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • CHES 0.1 M pH 9
  • Polyethylene glycol 8,000 20 w/v -
pH 9
100.0 μl of CHES pH 9 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
A3
  • Lithium sulfate 0.05 M -
  • Sodium sulfate 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
18.5 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium sulfate (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
581.5 μl of water (100 v/v)
A4
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
A5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 1,6-Hexanediol 3.4 v/v -
pH 8.5
47.9 μl of 1,6-Hexanediol (71 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
772.1 μl of water (100 v/v)
A6
  • Magnesium chloride 0.05 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 40 v/v -
pH 8.5
400.0 μl of Ethanol (100 v/v)
20.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
480.0 μl of water (100 v/v)
A7
  • tri-Potassium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate (2.5 M)
520.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A9
  • tri-Lithium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Lithium citrate (1.5 M)
466.7 μl of water (100 v/v)
A10
  • Calcium acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 1,000 20 w/v -
pH 8
200.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
300.0 μl of water (100 v/v)
A11
  • Potassium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Potassium acetate (5 M)
560.0 μl of water (100 v/v)
A12
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
58.8 μl of Magnesium acetate (3.4 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
541.2 μl of water (100 v/v)
B1
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
B2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
B4
  • Calcium chloride 0.19 M -
  • HEPES 0.095 M pH 7.5
  • Polyethylene glycol 400 26.6 v/v -
  • Glycerol 5 v/v -
pH 7.5
95.0 μl of Calcium chloride (2 M)
50.0 μl of Glycerol (100 v/v)
36.9 μl of HEPES pH 6.5 (1 M)
58.1 μl of HEPES pH 8 (1 M)
266.0 μl of Polyethylene glycol 400 (100 v/v)
494.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
B6
  • Sodium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
  • Potassium di-hydrogen phosphate 0.8 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
266.7 μl of Sodium di-hydrogen phosphate (3 M)
433.3 μl of water (100 v/v)
B7
  • di-Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of di-Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
B8
  • Calcium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B9
  • Potassium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.3 μl of Potassium formate (14 M)
585.7 μl of water (100 v/v)
B10
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
B11
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
B12
  • Potassium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Potassium fluoride (6 M)
566.7 μl of water (100 v/v)
C1
  • Ammonium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Lithium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
25.0 μl of Lithium nitrate (8 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
575.0 μl of water (100 v/v)
C3
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 5 w/v -
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
400.0 μl of water (100 v/v)
C4
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 8,000 10 w/v -
pH 7
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
620.0 μl of water (100 v/v)
C5
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,000 20 w/v -
pH 7
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
300.0 μl of water (100 v/v)
C6
  • Sodium chloride 2.5 M -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
320.0 μl of water (100 v/v)
C7
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
C8
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
533.3 μl of water (100 v/v)
C9
  • Potassium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
C10
  • HEPES 0.1 M pH 6.5
  • Polyethylene glycol 6,000 20 w/v -
pH 6.5
100.0 μl of HEPES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C11
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate (3 M)
533.3 μl of water (100 v/v)
C12
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
D1
  • Sodium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium iodide (1 M)
400.0 μl of water (100 v/v)
D2
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
D3
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
D4
  • Potassium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium iodide (1 M)
400.0 μl of water (100 v/v)
D5
  • Lithium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
D6
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 200 50 v/v -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
320.0 μl of water (100 v/v)
D7
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
D8
  • Sodium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
400.0 μl of water (100 v/v)
D9
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
D10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
D11
  • tri-Sodium citrate 1.6 M pH 6.5
pH 6.5
800.0 μl of tri-Sodium citrate pH 6.5 (2 M)
200.0 μl of water (100 v/v)
D12
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
58.8 μl of Magnesium acetate (3.4 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
441.2 μl of water (100 v/v)
E1
  • Ammonium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium nitrate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
E2
  • Ammonium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
E3
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 8,000 10 w/v -
pH 6.2
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
726.7 μl of water (100 v/v)
E4
  • Ammonium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Ammonium iodide (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
E5
  • Ammonium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
E6
  • MES 0.1 M pH 6
  • Polyethylene glycol 3,000 5 w/v -
  • Polyethylene glycol 200 30 v/v -
pH 6
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
300.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
500.0 μl of water (100 v/v)
E7
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 8,000 20 w/v -
pH 6
200.0 μl of Calcium acetate (1 M)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
300.0 μl of water (100 v/v)
E8
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
74.1 μl of Lithium sulfate (2.7 M)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
475.9 μl of water (100 v/v)
E9
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
E10
  • MES 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 5 (1 M)
500.0 μl of water (100 v/v)
E11
  • MES 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 5 (1 M)
700.0 μl of water (100 v/v)
E12
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
100.0 μl of MES pH 5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
F1
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
100.0 μl of MES pH 5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
F2
  • Magnesium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
80.0 μl of Magnesium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
F3
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F4
  • Magnesium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
66.7 μl of Magnesium nitrate (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
533.3 μl of water (100 v/v)
F5
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
F6
  • tri-Sodium citrate 0.095 M pH 5.6
  • 2-Propanol 19 v/v -
  • Polyethylene glycol 4,000 19 w/v -
  • Glycerol 5 v/v -
pH 5.6
190.0 μl of 2-Propanol (100 v/v)
50.0 μl of Glycerol (100 v/v)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
95.0 μl of tri-Sodium citrate pH 5.6 (1 M)
285.0 μl of water (100 v/v)
F7
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
F8
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
500.0 μl of water (100 v/v)
F9
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 200 50 v/v -
pH 4.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
360.0 μl of water (100 v/v)
F10
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 5 w/v -
  • Ethanol 40 v/v -
pH 4.2
400.0 μl of Ethanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
F11
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
  • Polyethylene glycol 400 50 v/v -
pH 4.5
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
325.9 μl of water (100 v/v)
F12
  • Sodium phosphate citrate 0.1 M pH 4.2
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 4.2
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
G1
  • tri-Ammonium citrate 0.18 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
72.0 μl of tri-Ammonium citrate (2.5 M)
528.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
700.0 μl of water (100 v/v)
G3
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
600.0 μl of water (100 v/v)
G4
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
G5
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
G6
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 5 w/v -
pH 4
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
800.0 μl of water (100 v/v)
G7
  • Potassium di-hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate (4 M)
550.0 μl of water (100 v/v)
G8
  • Ammonium di-hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
G9
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Ammonium sulfate 0.2 M -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
250.0 μl of water (100 v/v)
G10
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
G11
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
G12
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
H1
  • Sodium acetate 0.1 M pH 4.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 4.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
550.0 μl of water (100 v/v)
H2
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,000 20 w/v -
pH 4.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
500.0 μl of water (100 v/v)
H3
  • Sodium di-hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium di-hydrogen phosphate (3 M)
533.3 μl of water (100 v/v)
H4
  • Potassium di-hydrogen phosphate 0.05 M -
  • Polyethylene glycol 8,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
12.5 μl of Potassium di-hydrogen phosphate (4 M)
587.5 μl of water (100 v/v)
H5
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 3,000 10 w/v -
pH 4.2
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
660.0 μl of water (100 v/v)
H6
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Ammonium sulfate 2 M -
pH 4.2
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
H7
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 20 w/v -
pH 4.2
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
425.9 μl of water (100 v/v)
H8
  • tri-Sodium citrate 0.1 M pH 2.5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 2.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of tri-Sodium citrate pH 2.5 (2 M)
750.0 μl of water (100 v/v)
H9
  • tri-Sodium citrate 0.1 M pH 3.5
  • Ammonium sulfate 0.8 M -
pH 3.5
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
700.0 μl of water (100 v/v)
H10
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
400.0 μl of water (100 v/v)
H11
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 10 w/v -
pH 4
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
600.0 μl of water (100 v/v)
H12
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 10 w/v -
pH 4
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)