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Supplemental Information

Screen Description

JCSG Core I

Qiagen / JCSG Core I
Created 2009-11-16.

Export

JCSG Core I

Barcodes

JCSG Core I

    Screen Structure

    JCSG Core I

    Well Count 96
    Number of distinct ingredients 65
    Internal diversity (score mean dist) 0.924

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:07:23.363744 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG Core I
    Buffer 83
    Cryoprotectant 2
    Organic (non-volatile) 17
    Organic (volatile) 5
    Polymer 81
    Salt 101

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG Core I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • CHES 0.1 M pH 9
    • Polyethylene glycol 8,000 20 w/v -
    		pH 9
    100 μl of CHES pH 9 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    A2
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 8.5
    		pH 8.5
    31 μl of BICINE pH 7.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    68 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    A3
    • Lithium sulfate 0.05 M -
    • Sodium sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Sodium sulfate (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    18 μl of Lithium sulfate (2.7 M)
    581 μl of water (100 v/v)
    A4
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    		pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    320 μl of water (100 v/v)
    A5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 v/v -
    		pH 8.5
    47 μl of 1,6-Hexanediol (71 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    772 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 40 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ethanol (100 v/v)
    20 μl of Magnesium chloride (2.5 M)
    480 μl of water (100 v/v)
    A7
    • tri-Potassium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of tri-Potassium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    A9
    • tri-Lithium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    133 μl of tri-Lithium citrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    A10
    • Calcium acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 1,000 20 w/v -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    A11
    • Potassium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Potassium acetate (5 M)
    560 μl of water (100 v/v)
    A12
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    58 μl of Magnesium acetate (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    B1
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 7.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    38 μl of HEPES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    61 μl of HEPES pH 8 (1 M)
    460 μl of water (100 v/v)
    B2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    700 μl of water (100 v/v)
    B4
    • Calcium chloride 0.19 M -
    • HEPES 0.095 M pH 7.5
    • Polyethylene glycol 400 26.6 v/v -
    • Glycerol 5 v/v -
    		pH 7.5
    36 μl of HEPES pH 6.5 (1 M)
    58 μl of HEPES pH 8 (1 M)
    266 μl of Polyethylene glycol 400 (100 v/v)
    95 μl of Calcium chloride (2 M)
    50 μl of Glycerol (100 v/v)
    494 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • Sodium di-hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 0.8 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    266 μl of Sodium di-hydrogen phosphate (3 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Potassium di-hydrogen phosphate (4 M)
    433 μl of water (100 v/v)
    B7
    • di-Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    133 μl of di-Sodium tartrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    B8
    • Calcium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    B9
    • Potassium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    14 μl of Potassium formate (14 M)
    585 μl of water (100 v/v)
    B10
    • Potassium Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Potassium Sodium tartrate (2 M)
    500 μl of water (100 v/v)
    B11
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium formate (7 M)
    571 μl of water (100 v/v)
    B12
    • Potassium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    33 μl of Potassium fluoride (6 M)
    566 μl of water (100 v/v)
    C1
    • Ammonium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    400 μl of water (100 v/v)
    C2
    • Lithium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    25 μl of Lithium nitrate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    C3
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 5 w/v -
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    400 μl of water (100 v/v)
    C4
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 8,000 10 w/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    620 μl of water (100 v/v)
    C5
    • Calcium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,000 20 w/v -
    		pH 7
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    C6
    • Sodium chloride 2.5 M -
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    500 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    C7
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C8
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Sodium acetate (3 M)
    533 μl of water (100 v/v)
    C9
    • Potassium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    575 μl of water (100 v/v)
    C10
    • HEPES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 6.5
    100 μl of HEPES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    C11
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Potassium nitrate (3 M)
    533 μl of water (100 v/v)
    C12
    • Sodium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    25 μl of Sodium thiocyanate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    D1
    • Sodium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium iodide (1 M)
    400 μl of water (100 v/v)
    D2
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Potassium chloride (4 M)
    550 μl of water (100 v/v)
    D3
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    D4
    • Potassium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    200 μl of Potassium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D5
    • Lithium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Lithium chloride (10 M)
    580 μl of water (100 v/v)
    D6
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 200 50 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    D7
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Ammonium tartrate (2 M)
    500 μl of water (100 v/v)
    D8
    • Sodium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    400 μl of water (100 v/v)
    D9
    • Ammonium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium formate (10 M)
    580 μl of water (100 v/v)
    D10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 10 w/v -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    650 μl of water (100 v/v)
    D11
    • tri-Sodium citrate 1.6 M pH 6.5
    		pH 6.5
    800 μl of tri-Sodium citrate pH 6.5 (2 M)
    200 μl of water (100 v/v)
    D12
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    58 μl of Magnesium acetate (3.4 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    441 μl of water (100 v/v)
    E1
    • Ammonium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium nitrate (10 M)
    580 μl of water (100 v/v)
    E2
    • Ammonium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium chloride (5 M)
    560 μl of water (100 v/v)
    E3
    • Sodium chloride 0.2 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 8,000 10 w/v -
    		pH 6.2
    40 μl of Sodium chloride (5 M)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    726 μl of water (100 v/v)
    E4
    • Ammonium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    200 μl of Ammonium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    E5
    • Ammonium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    20 μl of Ammonium fluoride (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    E6
    • MES 0.1 M pH 6
    • Polyethylene glycol 3,000 5 w/v -
    • Polyethylene glycol 200 30 v/v -
    		pH 6
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    54 μl of MES pH 5 (1 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    45 μl of MES pH 7 (1 M)
    500 μl of water (100 v/v)
    E7
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 8,000 20 w/v -
    		pH 6
    54 μl of MES pH 5 (1 M)
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    45 μl of MES pH 7 (1 M)
    300 μl of water (100 v/v)
    E8
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    		pH 6
    54 μl of MES pH 5 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    74 μl of Lithium sulfate (2.7 M)
    45 μl of MES pH 7 (1 M)
    475 μl of water (100 v/v)
    E9
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    E10
    • MES 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    E11
    • MES 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    700 μl of water (100 v/v)
    E12
    • MES 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    F1
    • MES 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    F2
    • Magnesium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium sulfate (2.5 M)
    520 μl of water (100 v/v)
    F3
    • Magnesium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    200 μl of Magnesium formate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    F4
    • Magnesium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    66 μl of Magnesium nitrate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    F5
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    520 μl of water (100 v/v)
    F6
    • tri-Sodium citrate 0.095 M pH 5.6
    • 2-Propanol 19 v/v -
    • Polyethylene glycol 4,000 19 w/v -
    • Glycerol 5 v/v -
    		pH 5.6
    190 μl of 2-Propanol (100 v/v)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    95 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Glycerol (100 v/v)
    285 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.6
    200 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of water (100 v/v)
    F8
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 5.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    500 μl of water (100 v/v)
    F9
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 200 50 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    40 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    360 μl of water (100 v/v)
    F10
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 5 w/v -
    • Ethanol 40 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of Ethanol (100 v/v)
    400 μl of water (100 v/v)
    F11
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    • Polyethylene glycol 400 50 v/v -
    		pH 4.5
    500 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    325 μl of water (100 v/v)
    F12
    • Sodium phosphate citrate 0.1 M pH 4.2
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    G1
    • tri-Ammonium citrate 0.18 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    72 μl of tri-Ammonium citrate (2.5 M)
    528 μl of water (100 v/v)
    G2
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    700 μl of water (100 v/v)
    G3
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Lithium chloride (10 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    G4
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    G5
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)
    G6
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 5 w/v -
    		pH 4
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    G7
    • Potassium di-hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Potassium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    G8
    • Ammonium di-hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    520 μl of water (100 v/v)
    G9
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of water (100 v/v)
    G10
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    		pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    G11
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    50 μl of Ammonium sulfate (4 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    350 μl of water (100 v/v)
    G12
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    590 μl of water (100 v/v)
    H1
    • Sodium acetate 0.1 M pH 4.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    		pH 4.5
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    550 μl of water (100 v/v)
    H2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 4.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    500 μl of water (100 v/v)
    H3
    • Sodium di-hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    66 μl of Sodium di-hydrogen phosphate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    H4
    • Potassium di-hydrogen phosphate 0.05 M -
    • Polyethylene glycol 8,000 20 w/v -
    		NA
    12 μl of Potassium di-hydrogen phosphate (4 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    587 μl of water (100 v/v)
    H5
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 3,000 10 w/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    660 μl of water (100 v/v)
    H6
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Ammonium sulfate 2 M -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    H7
    • Lithium sulfate 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 20 w/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    425 μl of water (100 v/v)
    H8
    • tri-Sodium citrate 0.1 M pH 2.5
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 2.5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of tri-Sodium citrate pH 2.5 (2 M)
    750 μl of water (100 v/v)
    H9
    • tri-Sodium citrate 0.1 M pH 3.5
    • Ammonium sulfate 0.8 M -
    		pH 3.5
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    H10
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Lithium chloride (10 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of water (100 v/v)
    H11
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 10 w/v -
    		pH 4
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Lithium chloride (10 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    H12
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 10 w/v -
    		pH 4
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)