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Supplemental Information

Screen Description

JCSG Core II

Qiagen / JCSG Core II
Created 2009-11-16.

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JCSG Core II

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Screen Structure

JCSG Core II

Well Count 96
Number of distinct ingredients 54
Internal diversity (score mean dist) 0.954

Screen Composition

2025-08-16T01:39:05.212711 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG Core II
Buffer 92
Cryoprotectant 17
Organic (non-volatile) 40
Organic (volatile) 8
Polymer 62
Salt 112

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Core II Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium chloride 0.2 M -
  • CAPS 0.1 M pH 10
  • Polyethylene glycol 8,000 20 w/v -
pH 10
50.0 μl of CAPS pH 10 (1 M)
50.0 μl of CAPS pH 11 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
A2
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
  • Ammonium sulfate 1.26 M -
pH 9.5
315.0 μl of Ammonium sulfate (4 M)
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
40.0 μl of Sodium chloride (5 M)
545.0 μl of water (100 v/v)
A3
  • tri-Sodium citrate 1 M -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
A4
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 10 w/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
660.0 μl of water (100 v/v)
A5
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 20,000 10 w/v -
  • 1,4-Dioxane 2 v/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
333.3 μl of Polyethylene glycol 20,000 (30 w/v)
526.7 μl of water (100 v/v)
A6
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
A7
  • Lithium chloride 1 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 10 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
600.0 μl of water (100 v/v)
A8
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 5 w/v -
  • Polyethylene glycol 300 20 v/v -
  • Glycerol 10 v/v -
pH 8.5
100.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
A9
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
2.5 μl of Nickel (II) chloride (4 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
497.5 μl of water (100 v/v)
A10
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 20 v/v -
pH 8.5
200.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
A11
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
A12
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 40 v/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
425.9 μl of water (100 v/v)
B3
  • Calcium acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 8,000 10 w/v -
pH 8
200.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
B4
  • Magnesium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 8
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
470.0 μl of water (100 v/v)
B5
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 20 w/v -
pH 8.5
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B7
  • Lithium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
40.0 μl of Lithium acetate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B8
  • Magnesium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
420.0 μl of water (100 v/v)
B9
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Ethanol 15 v/v -
pH 7.5
150.0 μl of Ethanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
670.0 μl of water (100 v/v)
B10
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of water (100 v/v)
B11
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 4,000 17 w/v -
  • 2-Propanol 8.5 v/v -
  • Glycerol 15 v/v -
pH 7.5
85.0 μl of 2-Propanol (100 v/v)
150.0 μl of Glycerol (100 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)
B12
  • Potassium di-hydrogen phosphate 0.6 M -
  • HEPES 0.075 M pH 7.5
  • Glycerol 25 v/v -
  • Sodium di-hydrogen phosphate 0.6 M -
pH 7.5
250.0 μl of Glycerol (100 v/v)
75.0 μl of HEPES pH 7.5 (1 M)
150.0 μl of Potassium di-hydrogen phosphate (4 M)
200.0 μl of Sodium di-hydrogen phosphate (3 M)
325.0 μl of water (100 v/v)
C1
  • Magnesium chloride 0.2 M -
  • HEPES 0.09 M pH 7.5
  • Polyethylene glycol 400 27 v/v -
  • Glycerol 10 v/v -
pH 7.5
100.0 μl of Glycerol (100 v/v)
90.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
270.0 μl of Polyethylene glycol 400 (100 v/v)
460.0 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
  • Ammonium sulfate 2 M -
pH 7.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
380.0 μl of water (100 v/v)
C3
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
C4
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 200 50 v/v -
pH 6.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
426.7 μl of water (100 v/v)
C5
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
C6
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Ammonium sulfate 2 M -
pH 7
500.0 μl of Ammonium sulfate (4 M)
74.1 μl of Lithium sulfate (2.7 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
325.9 μl of water (100 v/v)
C7
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
300.0 μl of water (100 v/v)
C8
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 1,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
C9
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 10 w/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
600.0 μl of water (100 v/v)
C10
  • HEPES 0.1 M pH 6.5
  • Polyethylene glycol 6,000 10 w/v -
pH 6.5
100.0 μl of HEPES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C11
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 400 40 v/v -
pH 6.2
400.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
526.7 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 200 50 v/v -
pH 5.5
500.0 μl of Polyethylene glycol 200 (100 v/v)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.2 (1 M)
400.0 μl of water (100 v/v)
D1
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Glycerol 10 v/v -
  • 1,2-Propanediol 25 v/v -
pH 6.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
616.7 μl of water (100 v/v)
D2
  • Sodium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium nitrate (7 M)
571.4 μl of water (100 v/v)
D3
  • Lithium sulfate 0.05 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 200 50 v/v -
pH 7
18.5 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
381.5 μl of water (100 v/v)
D4
  • Potassium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium sulfate (0.5 M)
200.0 μl of water (100 v/v)
D5
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
800.0 μl of water (100 v/v)
D6
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 600 40 w/v -
pH 5.5
533.3 μl of Polyethylene glycol 600 (75 w/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
366.7 μl of water (100 v/v)
D7
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 1,000 20 w/v -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
420.0 μl of water (100 v/v)
D8
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 3,000 10 w/v -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
620.0 μl of water (100 v/v)
D9
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
74.1 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
525.9 μl of water (100 v/v)
D10
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
pH 6.5
500.0 μl of Ammonium sulfate (4 M)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
D11
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
D12
  • Lithium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
525.9 μl of water (100 v/v)
E1
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 1,000 20 w/v -
pH 6.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
526.7 μl of water (100 v/v)
E2
  • MES 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 5 (1 M)
800.0 μl of water (100 v/v)
E3
  • Lithium chloride 1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 20 w/v -
pH 6
100.0 μl of Lithium chloride (10 M)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of water (100 v/v)
E4
  • Lithium chloride 1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 10 w/v -
pH 6
100.0 μl of Lithium chloride (10 M)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
600.0 μl of water (100 v/v)
E5
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 5 w/v -
pH 5
100.0 μl of MES pH 5 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
E6
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 8
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
133.3 μl of Zinc acetate (1.5 M)
416.7 μl of water (100 v/v)
E7
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 600 40 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
533.3 μl of Polyethylene glycol 600 (75 w/v)
133.3 μl of Zinc acetate (1.5 M)
233.3 μl of water (100 v/v)
E8
  • Ammonium sulfate 0.5 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
  • Glycerol 10 v/v -
pH 7
125.0 μl of Ammonium sulfate (4 M)
100.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 600 (75 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
275.0 μl of water (100 v/v)
E9
  • Lithium sulfate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 0.5 M -
pH 5.6
125.0 μl of Ammonium sulfate (4 M)
370.4 μl of Lithium sulfate (2.7 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
404.6 μl of water (100 v/v)
E10
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
100.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
E12
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 300 40 v/v -
pH 4.5
400.0 μl of Polyethylene glycol 300 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
F1
  • Sodium acetate 0.1 M pH 4.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Glycerol 10 v/v -
pH 4.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Glycerol (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
450.0 μl of water (100 v/v)
F2
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
F3
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 5 w/v -
  • Ethylene glycol 50 v/v -
pH 4.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
300.0 μl of water (100 v/v)
F4
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 200 30 v/v -
pH 4.5
300.0 μl of Polyethylene glycol 200 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
F5
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
500.0 μl of water (100 v/v)
F6
  • Sodium acetate 0.1 M pH 4.5
  • Ethylene glycol 40 v/v -
pH 4.5
400.0 μl of Ethylene glycol (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
500.0 μl of water (100 v/v)
F7
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
800.0 μl of water (100 v/v)
F8
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 2.4 M -
pH 4
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
300.0 μl of water (100 v/v)
F9
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 1.6 M -
pH 4
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
500.0 μl of water (100 v/v)
F10
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 0.8 M -
pH 4
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
700.0 μl of water (100 v/v)
F11
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
400.0 μl of water (100 v/v)
F12
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 3,000 5 w/v -
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 4.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
450.0 μl of water (100 v/v)
G1
  • Ammonium sulfate 2 M -
  • 2-Propanol 5 v/v -
NA
50.0 μl of 2-Propanol (100 v/v)
500.0 μl of Ammonium sulfate (4 M)
450.0 μl of water (100 v/v)
G2
  • Ammonium sulfate 2 M -
NA
500.0 μl of Ammonium sulfate (4 M)
500.0 μl of water (100 v/v)
G3
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
pH 5.5
19.8 μl of MES pH 6.5 (1 M)
80.2 μl of MES pH 5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
420.0 μl of water (100 v/v)
G4
  • Cobalt (II) chloride 0.01 M -
  • Sodium acetate 0.1 M pH 4.6
  • 1,6-Hexanediol 1 v/v -
pH 4.6
14.1 μl of 1,6-Hexanediol (71 v/v)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
875.9 μl of water (100 v/v)
G5
  • Sodium acetate 0.08 M pH 4.6
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
pH 4.6
400.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
320.0 μl of water (100 v/v)
G6
  • Sodium acetate 0.07 M pH 4.6
  • Polyethylene glycol 4,000 5.6 w/v -
  • Glycerol 30 v/v -
pH 4.6
300.0 μl of Glycerol (100 v/v)
112.0 μl of Polyethylene glycol 4,000 (50 w/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
518.0 μl of water (100 v/v)
G7
  • Calcium chloride 0.14 M -
  • Sodium acetate 0.07 M pH 4.6
  • Glycerol 30 v/v -
  • 2-Propanol 14 v/v -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
70.0 μl of Calcium chloride (2 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
420.0 μl of water (100 v/v)
G8
  • Ammonium sulfate 0.16 M -
  • Sodium acetate 0.08 M pH 4.6
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 20 v/v -
pH 4.6
40.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
280.0 μl of water (100 v/v)
G9
  • Calcium chloride 0.18 M -
  • Sodium acetate 0.09 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 27 v/v -
  • Glycerol 10 v/v -
pH 4.6
270.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
90.0 μl of Calcium chloride (2 M)
100.0 μl of Glycerol (100 v/v)
90.0 μl of Sodium acetate pH 4.6 (1 M)
450.0 μl of water (100 v/v)
G10
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
G11
  • Zinc acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,000 10 w/v -
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
133.3 μl of Zinc acetate (1.5 M)
566.7 μl of water (100 v/v)
G12
  • Ammonium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 20 v/v -
  • Glycerol 10 v/v -
pH 4.2
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
550.0 μl of water (100 v/v)
H1
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
400.0 μl of water (100 v/v)
H2
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 8,000 30 w/v -
pH 4.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
225.9 μl of water (100 v/v)
H3
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
H4
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • 2-Propanol 10 v/v -
pH 4.2
100.0 μl of 2-Propanol (100 v/v)
74.1 μl of Lithium sulfate (2.7 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
725.9 μl of water (100 v/v)
H5
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 8,000 20 w/v -
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
H7
  • Ammonium sulfate 0.17 M -
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
NA
42.5 μl of Ammonium sulfate (4 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
297.5 μl of water (100 v/v)
H8
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
H9
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
160.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
840.0 μl of water (100 v/v)
H10
  • 1,4-Dioxane 35 v/v -
NA
700.0 μl of 1,4-Dioxane (50 v/v)
300.0 μl of water (100 v/v)
H11
  • tri-Sodium citrate 0.1 M pH 2.5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 2.5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
70.8 μl of tri-Sodium citrate pH 1 (1 M)
29.2 μl of tri-Sodium citrate pH 3 (1 M)
800.0 μl of water (100 v/v)
H12
  • tri-Sodium citrate 0.1 M pH 2.5
  • Polyethylene glycol 6,000 20 w/v -
pH 2.5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
70.8 μl of tri-Sodium citrate pH 1 (1 M)
29.2 μl of tri-Sodium citrate pH 3 (1 M)
500.0 μl of water (100 v/v)