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Supplemental Information

Screen Description

JCSG Core II

Qiagen / JCSG Core II
Created 2009-11-16.

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JCSG Core II

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JCSG Core II

    Screen Structure

    JCSG Core II

    Well Count 96
    Number of distinct ingredients 54
    Internal diversity (score mean dist) 0.954

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:18:57.006610 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG Core II
    Buffer 92
    Cryoprotectant 17
    Organic (non-volatile) 40
    Organic (volatile) 8
    Polymer 62
    Salt 112

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG Core II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 0.2 M -
    • CAPS 0.1 M pH 10
    • Polyethylene glycol 8,000 20 w/v -
    		pH 10
    50 μl of CAPS pH 10 (1 M)
    50 μl of CAPS pH 11 (1 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    A2
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Ammonium sulfate 1.26 M -
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    315 μl of Ammonium sulfate (4 M)
    40 μl of Sodium chloride (5 M)
    50 μl of CHES pH 10 (1 M)
    545 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 1 M -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    50 μl of CHES pH 10 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    A4
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    40 μl of Sodium chloride (5 M)
    50 μl of CHES pH 10 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    660 μl of water (100 v/v)
    A5
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 20,000 10 w/v -
    • 1,4-Dioxane 2 v/v -
    		pH 9
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    40 μl of 1,4-Dioxane (50 v/v)
    100 μl of BICINE pH 9 (1 M)
    526 μl of water (100 v/v)
    A6
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    		pH 9
    20 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    680 μl of water (100 v/v)
    A7
    • Lithium chloride 1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 10 w/v -
    		pH 9
    100 μl of Lithium chloride (10 M)
    100 μl of BICINE pH 9 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    A8
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 5 w/v -
    • Polyethylene glycol 300 20 v/v -
    • Glycerol 10 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Glycerol (100 v/v)
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    A9
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    		pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    2 μl of Nickel (II) chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    497 μl of water (100 v/v)
    A10
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ethanol (100 v/v)
    700 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of water (100 v/v)
    A12
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 8 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    740 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    B2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 40 v/v -
    		pH 8.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    425 μl of water (100 v/v)
    B3
    • Calcium acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 8,000 10 w/v -
    		pH 8
    200 μl of Calcium acetate (1 M)
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    B4
    • Magnesium chloride 0.2 M -
    • Imidazole 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    		pH 8
    80 μl of Magnesium chloride (2.5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    470 μl of water (100 v/v)
    B5
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    B7
    • Lithium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    40 μl of Lithium acetate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    B8
    • Magnesium chloride 0.2 M -
    • Imidazole 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 8
    80 μl of Magnesium chloride (2.5 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    420 μl of water (100 v/v)
    B9
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Ethanol 15 v/v -
    		pH 7.5
    150 μl of Ethanol (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    670 μl of water (100 v/v)
    B10
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    		pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of water (100 v/v)
    B11
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 4,000 17 w/v -
    • 2-Propanol 8.5 v/v -
    • Glycerol 15 v/v -
    		pH 7.5
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of 2-Propanol (100 v/v)
    150 μl of Glycerol (100 v/v)
    85 μl of HEPES pH 7.5 (1 M)
    340 μl of water (100 v/v)
    B12
    • Potassium di-hydrogen phosphate 0.6 M -
    • HEPES 0.075 M pH 7.5
    • Glycerol 25 v/v -
    • Sodium di-hydrogen phosphate 0.6 M -
    		pH 7.5
    150 μl of Potassium di-hydrogen phosphate (4 M)
    250 μl of Glycerol (100 v/v)
    200 μl of Sodium di-hydrogen phosphate (3 M)
    75 μl of HEPES pH 7.5 (1 M)
    325 μl of water (100 v/v)
    C1
    • Magnesium chloride 0.2 M -
    • HEPES 0.09 M pH 7.5
    • Polyethylene glycol 400 27 v/v -
    • Glycerol 10 v/v -
    		pH 7.5
    270 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Glycerol (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    90 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 v/v -
    • Ammonium sulfate 2 M -
    		pH 7.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    380 μl of water (100 v/v)
    C3
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    C4
    • Sodium chloride 0.2 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 200 50 v/v -
    		pH 6.2
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    40 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    426 μl of water (100 v/v)
    C5
    • Sodium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    250 μl of Sodium fluoride (0.8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    350 μl of water (100 v/v)
    C6
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Ammonium sulfate 2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    500 μl of Ammonium sulfate (4 M)
    325 μl of water (100 v/v)
    C7
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 300 40 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    300 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 1,000 20 w/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    500 μl of water (100 v/v)
    C9
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 10 w/v -
    		pH 7
    100 μl of Lithium chloride (10 M)
    77 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    600 μl of water (100 v/v)
    C10
    • HEPES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 6.5
    100 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C11
    • Sodium chloride 0.2 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 400 40 v/v -
    		pH 6.2
    400 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    40 μl of Sodium chloride (5 M)
    526 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 200 50 v/v -
    		pH 5.5
    5 μl of tri-Sodium citrate pH 5.2 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of water (100 v/v)
    D1
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Glycerol 10 v/v -
    • 1,2-Propanediol 25 v/v -
    		pH 6.2
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    100 μl of Glycerol (100 v/v)
    250 μl of 1,2-Propanediol (100 v/v)
    616 μl of water (100 v/v)
    D2
    • Sodium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    28 μl of Sodium nitrate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.05 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 200 50 v/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    18 μl of Lithium sulfate (2.7 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    381 μl of water (100 v/v)
    D4
    • Potassium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Potassium sulfate (0.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    D5
    • Magnesium formate 0.2 M -
    		NA
    200 μl of Magnesium formate (1 M)
    800 μl of water (100 v/v)
    D6
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 600 40 w/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    533 μl of Polyethylene glycol 600 (75 w/v)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    366 μl of water (100 v/v)
    D7
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 1,000 20 w/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    420 μl of water (100 v/v)
    D8
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 3,000 10 w/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    620 μl of water (100 v/v)
    D9
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    525 μl of water (100 v/v)
    D10
    • Sodium cacodylate 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    • Sodium chloride 0.2 M -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    500 μl of Ammonium sulfate (4 M)
    40 μl of Sodium chloride (5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    360 μl of water (100 v/v)
    D11
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    		pH 6.5
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    D12
    • Lithium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    74 μl of Lithium sulfate (2.7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    525 μl of water (100 v/v)
    E1
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 1,000 20 w/v -
    		pH 6.2
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    526 μl of water (100 v/v)
    E2
    • MES 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    800 μl of water (100 v/v)
    E3
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    		pH 6
    100 μl of Lithium chloride (10 M)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    E4
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 10 w/v -
    		pH 6
    100 μl of Lithium chloride (10 M)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    E5
    • MES 0.1 M pH 5
    • Polyethylene glycol 6,000 5 w/v -
    		pH 5
    100 μl of MES pH 5 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    800 μl of water (100 v/v)
    E6
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    		pH 8
    133 μl of Zinc acetate (1.5 M)
    100 μl of Glycerol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    250 μl of 1,2-Propanediol (100 v/v)
    416 μl of water (100 v/v)
    E7
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 600 40 w/v -
    		pH 8
    133 μl of Zinc acetate (1.5 M)
    100 μl of Imidazole pH 8 (1 M)
    533 μl of Polyethylene glycol 600 (75 w/v)
    233 μl of water (100 v/v)
    E8
    • Ammonium sulfate 0.5 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    • Glycerol 10 v/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Glycerol (100 v/v)
    125 μl of Ammonium sulfate (4 M)
    400 μl of Polyethylene glycol 600 (75 v/v)
    275 μl of water (100 v/v)
    E9
    • Lithium sulfate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 0.5 M -
    		pH 5.6
    370 μl of Lithium sulfate (2.7 M)
    125 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    404 μl of water (100 v/v)
    E10
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium acetate 0.2 M -
    		pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of water (100 v/v)
    E11
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    E12
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 40 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    40 μl of Sodium chloride (5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    460 μl of water (100 v/v)
    F1
    • Sodium acetate 0.1 M pH 4.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Glycerol 10 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    100 μl of Glycerol (100 v/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    450 μl of water (100 v/v)
    F2
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    500 μl of water (100 v/v)
    F3
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,000 5 w/v -
    • Ethylene glycol 50 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Ethylene glycol (100 v/v)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    300 μl of water (100 v/v)
    F4
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 200 30 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    20 μl of Sodium chloride (5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    580 μl of water (100 v/v)
    F5
    • Sodium acetate 0.1 M pH 4.5
    • 1,2-Propanediol 40 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    500 μl of water (100 v/v)
    F6
    • Sodium acetate 0.1 M pH 4.5
    • Ethylene glycol 40 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Ethylene glycol (100 v/v)
    500 μl of water (100 v/v)
    F7
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 5
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    800 μl of water (100 v/v)
    F8
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 2.4 M -
    		pH 4
    600 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    300 μl of water (100 v/v)
    F9
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 1.6 M -
    		pH 4
    400 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    500 μl of water (100 v/v)
    F10
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    		pH 4
    200 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    700 μl of water (100 v/v)
    F11
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    F12
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 3,000 5 w/v -
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Glycerol (100 v/v)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    250 μl of 1,2-Propanediol (100 v/v)
    450 μl of water (100 v/v)
    G1
    • Ammonium sulfate 2 M -
    • 2-Propanol 5 v/v -
    		NA
    50 μl of 2-Propanol (100 v/v)
    500 μl of Ammonium sulfate (4 M)
    450 μl of water (100 v/v)
    G2
    • Ammonium sulfate 2 M -
    		NA
    500 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    G3
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    		pH 5.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of MES pH 5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    19 μl of MES pH 6.5 (1 M)
    420 μl of water (100 v/v)
    G4
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.1 M pH 4.6
    • 1,6-Hexanediol 1 v/v -
    		pH 4.6
    14 μl of 1,6-Hexanediol (71 v/v)
    10 μl of Cobalt (II) chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    875 μl of water (100 v/v)
    G5
    • Sodium acetate 0.08 M pH 4.6
    • Ammonium sulfate 1.6 M -
    • Glycerol 20 v/v -
    		pH 4.6
    200 μl of Glycerol (100 v/v)
    400 μl of Ammonium sulfate (4 M)
    80 μl of Sodium acetate pH 4.6 (1 M)
    320 μl of water (100 v/v)
    G6
    • Sodium acetate 0.07 M pH 4.6
    • Polyethylene glycol 4,000 5.6 w/v -
    • Glycerol 30 v/v -
    		pH 4.6
    112 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    518 μl of water (100 v/v)
    G7
    • Calcium chloride 0.14 M -
    • Sodium acetate 0.07 M pH 4.6
    • Glycerol 30 v/v -
    • 2-Propanol 14 v/v -
    		pH 4.6
    140 μl of 2-Propanol (100 v/v)
    70 μl of Calcium chloride (2 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    420 μl of water (100 v/v)
    G8
    • Ammonium sulfate 0.16 M -
    • Sodium acetate 0.08 M pH 4.6
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 20 v/v -
    		pH 4.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    40 μl of Ammonium sulfate (4 M)
    80 μl of Sodium acetate pH 4.6 (1 M)
    280 μl of water (100 v/v)
    G9
    • Calcium chloride 0.18 M -
    • Sodium acetate 0.09 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 27 v/v -
    • Glycerol 10 v/v -
    		pH 4.6
    90 μl of Calcium chloride (2 M)
    100 μl of Glycerol (100 v/v)
    90 μl of Sodium acetate pH 4.6 (1 M)
    270 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    450 μl of water (100 v/v)
    G10
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    500 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    G11
    • Zinc acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,000 10 w/v -
    		pH 4.5
    133 μl of Zinc acetate (1.5 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    566 μl of water (100 v/v)
    G12
    • Ammonium sulfate 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 20 v/v -
    • Glycerol 10 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Glycerol (100 v/v)
    200 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    H1
    • Calcium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 30 v/v -
    		pH 4.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    200 μl of Calcium acetate (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    H2
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 8,000 30 w/v -
    		pH 4.5
    74 μl of Lithium sulfate (2.7 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    225 μl of water (100 v/v)
    H3
    • Ethylene glycol 25 v/v -
    		NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    H4
    • Lithium sulfate 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • 2-Propanol 10 v/v -
    		pH 4.2
    74 μl of Lithium sulfate (2.7 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of 2-Propanol (100 v/v)
    725 μl of water (100 v/v)
    H5
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 8,000 20 w/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    H6
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    H7
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		NA
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    42 μl of Ammonium sulfate (4 M)
    297 μl of water (100 v/v)
    H8
    • Polyethylene glycol 1,500 30 w/v -
    		NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    H9
    • Ammonium di-hydrogen phosphate 0.4 M -
    		NA
    160 μl of Ammonium di-hydrogen phosphate (2.5 M)
    840 μl of water (100 v/v)
    H10
    • 1,4-Dioxane 35 v/v -
    		NA
    700 μl of 1,4-Dioxane (50 v/v)
    300 μl of water (100 v/v)
    H11
    • tri-Sodium citrate 0.1 M pH 2.5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 2.5
    75 μl of tri-Sodium citrate pH 3 (1 M)
    24 μl of tri-Sodium citrate pH 1 (1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    800 μl of water (100 v/v)
    H12
    • tri-Sodium citrate 0.1 M pH 2.5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 2.5
    75 μl of tri-Sodium citrate pH 3 (1 M)
    24 μl of tri-Sodium citrate pH 1 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)