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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG Core IV
Barcodes
JCSG Core IV
No barcodes found.
Screen Structure
JCSG Core IV
Well Count | 96 |
---|---|
Number of distinct ingredients | 49 |
Internal diversity (score mean dist) | 0.949 |
Screen Composition
ingredient type | JCSG Core IV |
---|---|
Buffer | 116 |
Cryoprotectant | 15 |
Organic (non-volatile) | 41 |
Organic (volatile) | 12 |
Polymer | 38 |
Salt | 104 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 10 | 500.0 μl of Ammonium sulfate (4 M) 50.0 μl of CAPS pH 10 (1 M) 50.0 μl of CAPS pH 11 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 325.9 μl of water (100 v/v) |
A2 |
| pH 10 | 100.0 μl of Glycine pH 10 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 200.0 μl of Potassium di-hydrogen phosphate (4 M) 400.0 μl of Sodium di-hydrogen phosphate (3 M) 225.9 μl of water (100 v/v) |
A3 |
| pH 10 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of CAPS pH 10 (1 M) 50.0 μl of CAPS pH 11 (1 M) 500.0 μl of water (100 v/v) |
A4 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 700.0 μl of water (100 v/v) |
A5 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Potassium Sodium tartrate (2 M) 325.9 μl of water (100 v/v) |
A6 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 600.0 μl of water (100 v/v) |
A7 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 150.0 μl of Ethanol (100 v/v) 750.0 μl of water (100 v/v) |
A8 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 125.0 μl of tri-Sodium citrate (1.6 M) 375.0 μl of water (100 v/v) |
A9 |
| pH 9.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 500.0 μl of water (100 v/v) |
A10 |
| pH 9 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 500.0 μl of water (100 v/v) |
A11 |
| pH 9 | 200.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 700.0 μl of water (100 v/v) |
A12 |
| pH 9 | 600.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 300.0 μl of water (100 v/v) |
B1 |
| pH 8.5 | 31.6 μl of BICINE pH 7.5 (1 M) 68.4 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
B2 |
| pH 9 | 600.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 300.0 μl of water (100 v/v) |
B3 |
| pH 8.5 | 31.6 μl of BICINE pH 7.5 (1 M) 68.4 μl of BICINE pH 9 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
B4 |
| pH 8.5 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 31.6 μl of BICINE pH 7.5 (1 M) 68.4 μl of BICINE pH 9 (1 M) 250.0 μl of water (100 v/v) |
B5 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of Magnesium chloride (2.5 M) 100.0 μl of water (100 v/v) |
B6 |
| pH 8.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 800.0 μl of water (100 v/v) |
B7 |
| pH 8.5 | 500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
B8 |
| pH 8.5 | 250.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Glycerol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 470.0 μl of water (100 v/v) |
B9 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
B10 |
| pH 8.5 | 300.0 μl of Polyethylene glycol 400 (100 v/v) 125.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 475.0 μl of water (100 v/v) |
B11 |
| pH 8.5 | 74.1 μl of Lithium sulfate (2.7 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 225.9 μl of water (100 v/v) |
B12 |
| pH 8.5 | 300.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
C1 |
| pH 8.5 | 375.0 μl of Ammonium sulfate (4 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 405.0 μl of water (100 v/v) |
C2 |
| pH 8.5 | 300.0 μl of Polyethylene glycol 400 (100 v/v) 125.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 475.0 μl of water (100 v/v) |
C3 |
| pH 8.5 | 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 56.7 μl of Sodium acetate (3 M) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 198.3 μl of water (100 v/v) |
C4 |
| pH 8 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 800.0 μl of water (100 v/v) |
C5 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium chloride (5 M) 133.3 μl of Zinc acetate (1.5 M) 266.7 μl of water (100 v/v) |
C6 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium chloride (5 M) 400.0 μl of water (100 v/v) |
C7 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v) |
C8 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 40.0 μl of Sodium chloride (5 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 574.3 μl of water (100 v/v) |
C9 |
| pH 8.5 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
C10 |
| pH 8.5 | 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 800.0 μl of water (100 v/v) |
C11 |
| pH 8.5 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
C12 |
| pH 8.5 | 100.0 μl of Lithium chloride (10 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
D1 |
| pH 8 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 100.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 315.0 μl of Ammonium sulfate (4 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 585.0 μl of water (100 v/v) |
D3 |
| pH 7.5 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 40.0 μl of Sodium chloride (5 M) 510.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 555.6 μl of Lithium sulfate (2.7 M) 344.4 μl of water (100 v/v) |
D6 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 860.0 μl of Sodium chloride (5 M) 40.0 μl of water (100 v/v) |
D7 |
| pH 7.5 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 125.0 μl of tri-Sodium citrate (1.6 M) 475.0 μl of water (100 v/v) |
D8 |
| pH 7.5 | 80.0 μl of Ethylene glycol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 420.0 μl of water (100 v/v) |
D9 |
| pH 7.5 | 100.0 μl of Glycerol (100 v/v) 35.0 μl of HEPES pH 6.5 (1 M) 55.0 μl of HEPES pH 8 (1 M) 787.5 μl of tri-Sodium citrate (1.6 M) 22.5 μl of water (100 v/v) |
D10 |
| pH 7.5 | 425.0 μl of Ammonium sulfate (4 M) 150.0 μl of Glycerol (100 v/v) 33.0 μl of HEPES pH 6.5 (1 M) 52.0 μl of HEPES pH 8 (1 M) 17.0 μl of Polyethylene glycol 400 (100 v/v) 323.0 μl of water (100 v/v) |
D11 |
| pH 7.5 | 100.0 μl of Glycerol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 18.5 μl of Lithium sulfate (2.7 M) 400.0 μl of Polyethylene glycol 600 (75 w/v) 381.5 μl of water (100 v/v) |
D12 |
| pH 7.5 | 300.0 μl of 1,2-Propanediol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v) |
E1 |
| pH 7 | 250.0 μl of 1,2-Propanediol (100 v/v) 50.0 μl of Ammonium sulfate (4 M) 100.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 500.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 400.0 μl of Ethylene glycol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 400.0 μl of water (100 v/v) |
E3 |
| pH 7 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 450.0 μl of water (100 v/v) |
E4 |
| NA | 571.4 μl of Sodium formate (7 M) 428.6 μl of water (100 v/v) |
E5 |
| NA | 100.0 μl of Glycerol (100 v/v) 514.3 μl of Sodium formate (7 M) 385.7 μl of water (100 v/v) |
E6 |
| pH 7.5 | 200.0 μl of Calcium acetate (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 300.0 μl of water (100 v/v) |
E7 |
| pH 7 | 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 260.0 μl of water (100 v/v) |
E8 |
| pH 7 | 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Potassium Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 325.9 μl of water (100 v/v) |
E9 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 533.3 μl of Polyethylene glycol 600 (75 w/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 166.7 μl of water (100 v/v) |
E10 |
| pH 6.5 | 200.0 μl of Ammonium sulfate (4 M) 100.0 μl of HEPES pH 6.5 (1 M) 700.0 μl of water (100 v/v) |
E11 |
| pH 7 | 800.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 100.0 μl of water (100 v/v) |
E12 |
| pH 6.5 | 100.0 μl of HEPES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
F1 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 100.0 μl of Lithium chloride (10 M) 800.0 μl of water (100 v/v) |
F2 |
| pH 6.5 | 100.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 600 (75 w/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 200.0 μl of Sodium chloride (5 M) 200.0 μl of water (100 v/v) |
F3 |
| pH 6.5 | 100.0 μl of 2-Propanol (100 v/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 133.3 μl of Zinc acetate (1.5 M) 666.7 μl of water (100 v/v) |
F4 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 450.0 μl of Glycerol (100 v/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 250.0 μl of water (100 v/v) |
F5 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 300.0 μl of water (100 v/v) |
F6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 25.0 μl of Potassium di-hydrogen phosphate (4 M) 400.0 μl of Sodium chloride (5 M) 33.3 μl of Sodium di-hydrogen phosphate (3 M) 441.7 μl of water (100 v/v) |
F7 |
| pH 6.5 | 200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 38.5 μl of Sodium cacodylate pH 6.4 (1 M) 41.5 μl of Sodium cacodylate pH 6.6 (1 M) 106.7 μl of Zinc acetate (1.5 M) 325.3 μl of water (100 v/v) |
F8 |
| pH 5.5 | 300.0 μl of 1,2-Propanediol (100 v/v) 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 36.3 μl of tri-Sodium citrate pH 5.4 (1 M) 63.7 μl of tri-Sodium citrate pH 5.8 (1 M) 400.0 μl of water (100 v/v) |
F9 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of Zinc acetate (1.5 M) 466.7 μl of water (100 v/v) |
F10 |
| pH 5.5 | 350.0 μl of 2-Propanol (100 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 36.3 μl of tri-Sodium citrate pH 5.4 (1 M) 63.7 μl of tri-Sodium citrate pH 5.8 (1 M) 450.0 μl of water (100 v/v) |
F11 |
| pH 6 | 100.0 μl of Glycerol (100 v/v) 54.7 μl of MES pH 5 (1 M) 45.3 μl of MES pH 7 (1 M) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of Polyethylene glycol 600 (75 w/v) 300.0 μl of water (100 v/v) |
F12 |
| pH 5.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 36.3 μl of tri-Sodium citrate pH 5.4 (1 M) 63.7 μl of tri-Sodium citrate pH 5.8 (1 M) 500.0 μl of water (100 v/v) |
G1 |
| pH 8 | 350.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 133.3 μl of Zinc acetate (1.5 M) 416.7 μl of water (100 v/v) |
G2 |
| pH 6 | 54.7 μl of MES pH 5 (1 M) 45.3 μl of MES pH 7 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 400.0 μl of water (100 v/v) |
G3 |
| pH 6 | 200.0 μl of 1,4-Butanediol (100 v/v) 74.1 μl of Lithium sulfate (2.7 M) 54.7 μl of MES pH 5 (1 M) 45.3 μl of MES pH 7 (1 M) 625.9 μl of water (100 v/v) |
G4 |
| pH 6 | 150.0 μl of Ethanol (100 v/v) 54.7 μl of MES pH 5 (1 M) 45.3 μl of MES pH 7 (1 M) 133.3 μl of Zinc acetate (1.5 M) 616.7 μl of water (100 v/v) |
G5 |
| pH 5 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of MES pH 5 (1 M) 500.0 μl of water (100 v/v) |
G6 |
| pH 5 | 100.0 μl of MES pH 5 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
G7 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 133.3 μl of Zinc acetate (1.5 M) 366.7 μl of water (100 v/v) |
G8 |
| pH 5.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v) |
G9 |
| pH 5.6 | 10.0 μl of Iron (III) chloride (1 M) 200.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 690.0 μl of water (100 v/v) |
G10 |
| pH 5.6 | 280.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of tri-Sodium citrate pH 5.6 (1 M) 350.0 μl of water (100 v/v) |
G11 |
| pH 5.5 | 150.0 μl of Ethanol (100 v/v) 74.1 μl of Lithium sulfate (2.7 M) 36.3 μl of tri-Sodium citrate pH 5.4 (1 M) 63.7 μl of tri-Sodium citrate pH 5.8 (1 M) 675.9 μl of water (100 v/v) |
G12 |
| pH 4.5 | 400.0 μl of 1,2-Propanediol (100 v/v) 50.0 μl of Calcium acetate (1 M) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 450.0 μl of water (100 v/v) |
H1 |
| pH 4.5 | 350.0 μl of 2-Propanol (100 v/v) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 550.0 μl of water (100 v/v) |
H2 |
| pH 4.6 | 200.0 μl of Ammonium acetate (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of water (100 v/v) |
H3 |
| pH 4.6 | 170.0 μl of Ammonium acetate (1 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of Sodium acetate pH 4.6 (1 M) 85.0 μl of water (100 v/v) |
H4 |
| pH 4.5 | 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 133.3 μl of Zinc acetate (1.5 M) 366.7 μl of water (100 v/v) |
H5 |
| pH 4.5 | 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 614.3 μl of water (100 v/v) |
H6 |
| pH 4.5 | 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 266.7 μl of Sodium di-hydrogen phosphate (3 M) 300.0 μl of di-Potassium hydrogen phosphate (4 M) 333.3 μl of water (100 v/v) |
H7 |
| pH 4.2 | 50.0 μl of Ammonium sulfate (4 M) 400.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 450.0 μl of water (100 v/v) |
H8 |
| NA | 100.0 μl of Ethanol (100 v/v) 300.0 μl of Sodium chloride (5 M) 600.0 μl of water (100 v/v) |
H9 |
| NA | 375.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 375.0 μl of water (100 v/v) |
H10 |
| pH 4.2 | 533.3 μl of Sodium di-hydrogen phosphate (3 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 100.0 μl of di-Potassium hydrogen phosphate (4 M) 266.7 μl of water (100 v/v) |
H11 |
| pH 2.5 | 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 70.8 μl of tri-Sodium citrate pH 1 (1 M) 29.2 μl of tri-Sodium citrate pH 3 (1 M) 300.0 μl of water (100 v/v) |
H12 |
| pH 4 | 100.0 μl of Lithium chloride (10 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 200.0 μl of water (100 v/v) |