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Supplemental Information

Screen Description

JCSG Core IV

Qiagen / JCSG Core IV
Created 2009-11-16.

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Barcodes

JCSG Core IV

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Screen Structure

JCSG Core IV

Well Count 96
Number of distinct ingredients 49
Internal diversity (score mean dist) 0.949

Screen Composition

2025-08-16T09:05:38.766155 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG Core IV
Buffer 116
Cryoprotectant 15
Organic (non-volatile) 41
Organic (volatile) 12
Polymer 38
Salt 104

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Core IV Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Lithium sulfate 0.2 M -
  • CAPS 0.1 M pH 10
  • Ammonium sulfate 2 M -
pH 10
500.0 μl of Ammonium sulfate (4 M)
50.0 μl of CAPS pH 10 (1 M)
50.0 μl of CAPS pH 11 (1 M)
74.1 μl of Lithium sulfate (2.7 M)
325.9 μl of water (100 v/v)
A2
  • Sodium di-hydrogen phosphate 1.2 M -
  • Potassium di-hydrogen phosphate 0.8 M -
  • Lithium sulfate 0.2 M -
  • Glycine 0.1 M pH 10
pH 10
100.0 μl of Glycine pH 10 (1 M)
74.1 μl of Lithium sulfate (2.7 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
400.0 μl of Sodium di-hydrogen phosphate (3 M)
225.9 μl of water (100 v/v)
A3
  • CAPS 0.1 M pH 10
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 10
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CAPS pH 10 (1 M)
50.0 μl of CAPS pH 11 (1 M)
500.0 μl of water (100 v/v)
A4
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 3,000 10 w/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
700.0 μl of water (100 v/v)
A5
  • Potassium Sodium tartrate 1 M -
  • Lithium sulfate 0.2 M -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Potassium Sodium tartrate (2 M)
325.9 μl of water (100 v/v)
A6
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 400 30 v/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
A7
  • CHES 0.1 M pH 9.5
  • Ethanol 15 v/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
150.0 μl of Ethanol (100 v/v)
750.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.2 M -
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 300 40 v/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
375.0 μl of water (100 v/v)
A9
  • CHES 0.1 M pH 9.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 9.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
500.0 μl of water (100 v/v)
A10
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 1.6 M -
pH 9
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
500.0 μl of water (100 v/v)
A11
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 0.8 M -
pH 9
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
700.0 μl of water (100 v/v)
A12
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2.4 M -
pH 9
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of water (100 v/v)
B1
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 10 w/v -
pH 8.5
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
B2
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2.4 M -
pH 9
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of water (100 v/v)
B3
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 30 w/v -
pH 8.5
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
B4
  • BICINE 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
31.6 μl of BICINE pH 7.5 (1 M)
68.4 μl of BICINE pH 9 (1 M)
250.0 μl of water (100 v/v)
B5
  • Magnesium chloride 2 M -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 10 v/v -
pH 8.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
B7
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ethylene glycol 50 v/v -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B8
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 8.5
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
470.0 μl of water (100 v/v)
B9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
B10
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
B11
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
225.9 μl of water (100 v/v)
B12
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 30 v/v -
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C1
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.5 M -
  • Glycerol 12 v/v -
pH 8.5
375.0 μl of Ammonium sulfate (4 M)
120.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
405.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
56.7 μl of Sodium acetate (3 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
198.3 μl of water (100 v/v)
C4
  • Imidazole 0.1 M pH 8
  • 2-Propanol 10 v/v -
pH 8
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
C5
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Sodium chloride 2.5 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium chloride (5 M)
133.3 μl of Zinc acetate (1.5 M)
266.7 μl of water (100 v/v)
C6
  • Imidazole 0.1 M pH 8
  • Sodium chloride 2.5 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
C7
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 8,000 10 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
C8
  • di-Ammonium hydrogen phosphate 1 M -
  • Imidazole 0.1 M pH 8
  • Sodium chloride 0.2 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
574.3 μl of water (100 v/v)
C9
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.6 M -
pH 8.5
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C10
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 5 w/v -
pH 8.5
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
C11
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
C12
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 10 w/v -
pH 8.5
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
D1
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 3.2 M -
pH 8
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.26 M -
pH 7.5
315.0 μl of Ammonium sulfate (4 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
585.0 μl of water (100 v/v)
D3
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 7.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
510.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 200 50 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of water (100 v/v)
D5
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
555.6 μl of Lithium sulfate (2.7 M)
344.4 μl of water (100 v/v)
D6
  • Sodium chloride 4.3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)
D7
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
D8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
420.0 μl of water (100 v/v)
D9
  • tri-Sodium citrate 1.26 M -
  • HEPES 0.09 M pH 7.5
  • Glycerol 10 v/v -
pH 7.5
100.0 μl of Glycerol (100 v/v)
35.0 μl of HEPES pH 6.5 (1 M)
55.0 μl of HEPES pH 8 (1 M)
787.5 μl of tri-Sodium citrate (1.6 M)
22.5 μl of water (100 v/v)
D10
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 400 1.7 v/v -
  • Ammonium sulfate 1.7 M -
  • Glycerol 15 v/v -
pH 7.5
425.0 μl of Ammonium sulfate (4 M)
150.0 μl of Glycerol (100 v/v)
33.0 μl of HEPES pH 6.5 (1 M)
52.0 μl of HEPES pH 8 (1 M)
17.0 μl of Polyethylene glycol 400 (100 v/v)
323.0 μl of water (100 v/v)
D11
  • Lithium sulfate 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 30 w/v -
  • Glycerol 10 v/v -
pH 7.5
100.0 μl of Glycerol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
18.5 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 600 (75 w/v)
381.5 μl of water (100 v/v)
D12
  • HEPES 0.1 M pH 7.5
  • 1,2-Propanediol 30 v/v -
  • Polyethylene glycol 400 20 v/v -
pH 7.5
300.0 μl of 1,2-Propanediol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E1
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 7
250.0 μl of 1,2-Propanediol (100 v/v)
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
E2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 5 w/v -
  • Ethylene glycol 40 v/v -
pH 7.5
400.0 μl of Ethylene glycol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of water (100 v/v)
E3
  • tris chloride BCC 0.1 M pH 7
  • Ammonium sulfate 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 7
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
450.0 μl of water (100 v/v)
E4
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
E5
  • Sodium formate 3.6 M -
  • Glycerol 10 v/v -
NA
100.0 μl of Glycerol (100 v/v)
514.3 μl of Sodium formate (7 M)
385.7 μl of water (100 v/v)
E6
  • Calcium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 40 v/v -
pH 7.5
200.0 μl of Calcium acetate (1 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
E7
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,000 30 w/v -
pH 7
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E8
  • Potassium Sodium tartrate 1 M -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
325.9 μl of water (100 v/v)
E9
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 600 40 w/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
533.3 μl of Polyethylene glycol 600 (75 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
166.7 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 6.5
  • Ammonium sulfate 0.8 M -
pH 6.5
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 3.2 M -
pH 7
800.0 μl of Ammonium sulfate (4 M)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
100.0 μl of water (100 v/v)
E12
  • HEPES 0.1 M pH 6.5
  • Polyethylene glycol 6,000 30 w/v -
pH 6.5
100.0 μl of HEPES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
F1
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
100.0 μl of Lithium chloride (10 M)
800.0 μl of water (100 v/v)
F2
  • Sodium chloride 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 600 30 w/v -
  • Glycerol 10 v/v -
pH 6.5
100.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
200.0 μl of Sodium chloride (5 M)
200.0 μl of water (100 v/v)
F3
  • Zinc acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 10 v/v -
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
133.3 μl of Zinc acetate (1.5 M)
666.7 μl of water (100 v/v)
F4
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Glycerol 45 v/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
450.0 μl of Glycerol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
250.0 μl of water (100 v/v)
F5
  • HEPES 0.1 M pH 7
  • Jeffamine® M-600 30 v/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
300.0 μl of water (100 v/v)
F6
  • Sodium di-hydrogen phosphate 0.1 M -
  • Sodium chloride 2 M -
  • Potassium di-hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
25.0 μl of Potassium di-hydrogen phosphate (4 M)
400.0 μl of Sodium chloride (5 M)
33.3 μl of Sodium di-hydrogen phosphate (3 M)
441.7 μl of water (100 v/v)
F7
  • Zinc acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
pH 6.5
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
38.5 μl of Sodium cacodylate pH 6.4 (1 M)
41.5 μl of Sodium cacodylate pH 6.6 (1 M)
106.7 μl of Zinc acetate (1.5 M)
325.3 μl of water (100 v/v)
F8
  • tri-Sodium citrate 0.1 M pH 5.5
  • 1,2-Propanediol 30 v/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5.5
300.0 μl of 1,2-Propanediol (100 v/v)
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
400.0 μl of water (100 v/v)
F9
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Zinc acetate (1.5 M)
466.7 μl of water (100 v/v)
F10
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 5 w/v -
  • 2-Propanol 35 v/v -
pH 5.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
450.0 μl of water (100 v/v)
F11
  • MES 0.1 M pH 6
  • Glycerol 10 v/v -
  • Polyethylene glycol 600 30 w/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 6
100.0 μl of Glycerol (100 v/v)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
300.0 μl of water (100 v/v)
F12
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 5.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
500.0 μl of water (100 v/v)
G1
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • 2-Propanol 35 v/v -
pH 8
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
133.3 μl of Zinc acetate (1.5 M)
416.7 μl of water (100 v/v)
G2
  • Potassium Sodium tartrate 1 M -
  • MES 0.1 M pH 6
pH 6
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
500.0 μl of Potassium Sodium tartrate (2 M)
400.0 μl of water (100 v/v)
G3
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
  • 1,4-Butanediol 20 v/v -
pH 6
200.0 μl of 1,4-Butanediol (100 v/v)
74.1 μl of Lithium sulfate (2.7 M)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
625.9 μl of water (100 v/v)
G4
  • Zinc acetate 0.2 M -
  • MES 0.1 M pH 6
  • Ethanol 15 v/v -
pH 6
150.0 μl of Ethanol (100 v/v)
54.7 μl of MES pH 5 (1 M)
45.3 μl of MES pH 7 (1 M)
133.3 μl of Zinc acetate (1.5 M)
616.7 μl of water (100 v/v)
G5
  • MES 0.1 M pH 5
  • Ammonium sulfate 1.6 M -
pH 5
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 5 (1 M)
500.0 μl of water (100 v/v)
G6
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 30 w/v -
pH 5
100.0 μl of MES pH 5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
G7
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 300 40 v/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
133.3 μl of Zinc acetate (1.5 M)
366.7 μl of water (100 v/v)
G8
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G9
  • Iron (III) chloride 0.01 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Jeffamine® M-600 10 v/v -
pH 5.6
10.0 μl of Iron (III) chloride (1 M)
200.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
G10
  • Ammonium di-hydrogen phosphate 0.7 M -
  • tri-Sodium citrate 0.07 M pH 5.6
  • Glycerol 30 v/v -
pH 5.6
280.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of tri-Sodium citrate pH 5.6 (1 M)
350.0 μl of water (100 v/v)
G11
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Ethanol 15 v/v -
pH 5.5
150.0 μl of Ethanol (100 v/v)
74.1 μl of Lithium sulfate (2.7 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
675.9 μl of water (100 v/v)
G12
  • Calcium acetate 0.05 M -
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
50.0 μl of Calcium acetate (1 M)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
450.0 μl of water (100 v/v)
H1
  • Sodium acetate 0.1 M pH 4.5
  • 2-Propanol 35 v/v -
pH 4.5
350.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
550.0 μl of water (100 v/v)
H2
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
H3
  • Ammonium acetate 0.17 M -
  • Sodium acetate 0.085 M pH 4.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 4.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate pH 4.6 (1 M)
85.0 μl of water (100 v/v)
H4
  • Zinc acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 20 w/v -
pH 4.5
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
133.3 μl of Zinc acetate (1.5 M)
366.7 μl of water (100 v/v)
H5
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
614.3 μl of water (100 v/v)
H6
  • Sodium di-hydrogen phosphate 0.8 M -
  • di-Potassium hydrogen phosphate 1.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
266.7 μl of Sodium di-hydrogen phosphate (3 M)
300.0 μl of di-Potassium hydrogen phosphate (4 M)
333.3 μl of water (100 v/v)
H7
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Ammonium sulfate 0.2 M -
  • Ethylene glycol 40 v/v -
pH 4.2
50.0 μl of Ammonium sulfate (4 M)
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
450.0 μl of water (100 v/v)
H8
  • Sodium chloride 1.5 M -
  • Ethanol 10 v/v -
NA
100.0 μl of Ethanol (100 v/v)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
H9
  • Ammonium sulfate 1.5 M -
  • Glycerol 25 v/v -
NA
375.0 μl of Ammonium sulfate (4 M)
250.0 μl of Glycerol (100 v/v)
375.0 μl of water (100 v/v)
H10
  • Sodium di-hydrogen phosphate 1.6 M -
  • di-Potassium hydrogen phosphate 0.4 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
533.3 μl of Sodium di-hydrogen phosphate (3 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
100.0 μl of di-Potassium hydrogen phosphate (4 M)
266.7 μl of water (100 v/v)
H11
  • tri-Sodium citrate 0.1 M pH 2.5
  • Polyethylene glycol 6,000 30 w/v -
pH 2.5
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
70.8 μl of tri-Sodium citrate pH 1 (1 M)
29.2 μl of tri-Sodium citrate pH 3 (1 M)
300.0 μl of water (100 v/v)
H12
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 30 w/v -
pH 4
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
200.0 μl of water (100 v/v)