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Supplemental Information

Screen Description

JCSG Core IV

Qiagen / JCSG Core IV
Created 2009-11-16.

Export

JCSG Core IV

Barcodes

JCSG Core IV

    Screen Structure

    JCSG Core IV

    Well Count 96
    Number of distinct ingredients 49
    Internal diversity (score mean dist) 0.949

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:33:17.220430 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG Core IV
    Buffer 116
    Cryoprotectant 15
    Organic (non-volatile) 41
    Organic (volatile) 12
    Polymer 38
    Salt 104

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG Core IV Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium sulfate 0.2 M -
    • CAPS 0.1 M pH 10
    • Ammonium sulfate 2 M -
    		pH 10
    74 μl of Lithium sulfate (2.7 M)
    500 μl of Ammonium sulfate (4 M)
    50 μl of CAPS pH 11 (1 M)
    50 μl of CAPS pH 10 (1 M)
    325 μl of water (100 v/v)
    A2
    • Sodium di-hydrogen phosphate 1.2 M -
    • Potassium di-hydrogen phosphate 0.8 M -
    • Lithium sulfate 0.2 M -
    • Glycine 0.1 M pH 10
    		pH 10
    74 μl of Lithium sulfate (2.7 M)
    400 μl of Sodium di-hydrogen phosphate (3 M)
    200 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of Glycine pH 10 (1 M)
    225 μl of water (100 v/v)
    A3
    • CAPS 0.1 M pH 10
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 10
    50 μl of CAPS pH 11 (1 M)
    50 μl of CAPS pH 10 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    A4
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 3,000 10 w/v -
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of CHES pH 10 (1 M)
    700 μl of water (100 v/v)
    A5
    • Potassium Sodium tartrate 1 M -
    • Lithium sulfate 0.2 M -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    74 μl of Lithium sulfate (2.7 M)
    500 μl of Potassium Sodium tartrate (2 M)
    50 μl of CHES pH 9 (1 M)
    50 μl of CHES pH 10 (1 M)
    325 μl of water (100 v/v)
    A6
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 400 30 v/v -
    		pH 9.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of CHES pH 9 (1 M)
    50 μl of CHES pH 10 (1 M)
    600 μl of water (100 v/v)
    A7
    • CHES 0.1 M pH 9.5
    • Ethanol 15 v/v -
    		pH 9.5
    150 μl of Ethanol (100 v/v)
    50 μl of CHES pH 9 (1 M)
    50 μl of CHES pH 10 (1 M)
    750 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 300 40 v/v -
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of CHES pH 10 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    375 μl of water (100 v/v)
    A9
    • CHES 0.1 M pH 9.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 9.5
    50 μl of CHES pH 9 (1 M)
    50 μl of CHES pH 10 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    A10
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 1.6 M -
    		pH 9
    400 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    A11
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 0.8 M -
    		pH 9
    200 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    A12
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    		pH 9
    600 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)
    B1
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 8.5
    31 μl of BICINE pH 7.5 (1 M)
    68 μl of BICINE pH 9 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    B2
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    		pH 9
    600 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)
    B3
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 8.5
    31 μl of BICINE pH 7.5 (1 M)
    68 μl of BICINE pH 9 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    B4
    • BICINE 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 8.5
    31 μl of BICINE pH 7.5 (1 M)
    68 μl of BICINE pH 9 (1 M)
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    B5
    • Magnesium chloride 2 M -
    • BICINE 0.1 M pH 9
    		pH 9
    800 μl of Magnesium chloride (2.5 M)
    100 μl of BICINE pH 9 (1 M)
    100 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 10 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    800 μl of water (100 v/v)
    B7
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ethylene glycol 50 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Ethylene glycol (100 v/v)
    320 μl of water (100 v/v)
    B8
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    		pH 8.5
    100 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    250 μl of 1,2-Propanediol (100 v/v)
    470 μl of water (100 v/v)
    B9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    220 μl of water (100 v/v)
    B10
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    B11
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    		pH 8.5
    74 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    225 μl of water (100 v/v)
    B12
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 30 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    200 μl of Ammonium acetate (1 M)
    400 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.5 M -
    • Glycerol 12 v/v -
    		pH 8.5
    375 μl of Ammonium sulfate (4 M)
    120 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    405 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    C3
    • Sodium acetate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 8.5
    150 μl of Glycerol (100 v/v)
    85 μl of tris chloride BCC pH 8.5 (1 M)
    56 μl of Sodium acetate (3 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    198 μl of water (100 v/v)
    C4
    • Imidazole 0.1 M pH 8
    • 2-Propanol 10 v/v -
    		pH 8
    100 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    800 μl of water (100 v/v)
    C5
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 2.5 M -
    		pH 8
    500 μl of Sodium chloride (5 M)
    133 μl of Zinc acetate (1.5 M)
    100 μl of Imidazole pH 8 (1 M)
    266 μl of water (100 v/v)
    C6
    • Imidazole 0.1 M pH 8
    • Sodium chloride 2.5 M -
    		pH 8
    500 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    400 μl of water (100 v/v)
    C7
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 8,000 10 w/v -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    700 μl of water (100 v/v)
    C8
    • di-Ammonium hydrogen phosphate 1 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of Imidazole pH 8 (1 M)
    574 μl of water (100 v/v)
    C9
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.6 M -
    		pH 8.5
    400 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C10
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 5 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    800 μl of water (100 v/v)
    C11
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    C12
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    D1
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 3.2 M -
    		pH 8
    800 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of water (100 v/v)
    D2
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.26 M -
    		pH 7.5
    315 μl of Ammonium sulfate (4 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    585 μl of water (100 v/v)
    D3
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    61 μl of HEPES pH 8 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    510 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 50 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of water (100 v/v)
    D5
    • Lithium sulfate 1.5 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    555 μl of Lithium sulfate (2.7 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    344 μl of water (100 v/v)
    D6
    • Sodium chloride 4.3 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    860 μl of Sodium chloride (5 M)
    61 μl of HEPES pH 8 (1 M)
    40 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    475 μl of water (100 v/v)
    D8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    • Ethylene glycol 8 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    61 μl of HEPES pH 8 (1 M)
    80 μl of Ethylene glycol (100 v/v)
    420 μl of water (100 v/v)
    D9
    • tri-Sodium citrate 1.26 M -
    • HEPES 0.09 M pH 7.5
    • Glycerol 10 v/v -
    		pH 7.5
    100 μl of Glycerol (100 v/v)
    35 μl of HEPES pH 6.5 (1 M)
    55 μl of HEPES pH 8 (1 M)
    787 μl of tri-Sodium citrate (1.6 M)
    22 μl of water (100 v/v)
    D10
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 400 1.7 v/v -
    • Ammonium sulfate 1.7 M -
    • Glycerol 15 v/v -
    		pH 7.5
    425 μl of Ammonium sulfate (4 M)
    150 μl of Glycerol (100 v/v)
    17 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of HEPES pH 6.5 (1 M)
    52 μl of HEPES pH 8 (1 M)
    323 μl of water (100 v/v)
    D11
    • Lithium sulfate 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 600 30 w/v -
    • Glycerol 10 v/v -
    		pH 7.5
    18 μl of Lithium sulfate (2.7 M)
    100 μl of Glycerol (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 600 (75 w/v)
    381 μl of water (100 v/v)
    D12
    • HEPES 0.1 M pH 7.5
    • 1,2-Propanediol 30 v/v -
    • Polyethylene glycol 400 20 v/v -
    		pH 7.5
    200 μl of Polyethylene glycol 400 (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    300 μl of 1,2-Propanediol (100 v/v)
    400 μl of water (100 v/v)
    E1
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    		pH 7
    50 μl of Ammonium sulfate (4 M)
    100 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    250 μl of 1,2-Propanediol (100 v/v)
    500 μl of water (100 v/v)
    E2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,000 5 w/v -
    • Ethylene glycol 40 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Ethylene glycol (100 v/v)
    400 μl of water (100 v/v)
    E3
    • tris chloride BCC 0.1 M pH 7
    • Ammonium sulfate 0.2 M -
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 7
    50 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    450 μl of water (100 v/v)
    E4
    • Sodium formate 4 M -
    		NA
    571 μl of Sodium formate (7 M)
    428 μl of water (100 v/v)
    E5
    • Sodium formate 3.6 M -
    • Glycerol 10 v/v -
    		NA
    100 μl of Glycerol (100 v/v)
    514 μl of Sodium formate (7 M)
    385 μl of water (100 v/v)
    E6
    • Calcium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 40 v/v -
    		pH 7.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    E7
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,000 30 w/v -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    260 μl of water (100 v/v)
    E8
    • Potassium Sodium tartrate 1 M -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    74 μl of Lithium sulfate (2.7 M)
    500 μl of Potassium Sodium tartrate (2 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    325 μl of water (100 v/v)
    E9
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 600 40 w/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    200 μl of Calcium acetate (1 M)
    533 μl of Polyethylene glycol 600 (75 w/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    166 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 6.5
    • Ammonium sulfate 0.8 M -
    		pH 6.5
    200 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 3.2 M -
    		pH 7
    800 μl of Ammonium sulfate (4 M)
    77 μl of HEPES pH 6.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    100 μl of water (100 v/v)
    E12
    • HEPES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 6.5
    100 μl of HEPES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    F1
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    100 μl of Lithium chloride (10 M)
    22 μl of HEPES pH 8 (1 M)
    800 μl of water (100 v/v)
    F2
    • Sodium chloride 1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 600 30 w/v -
    • Glycerol 10 v/v -
    		pH 6.5
    100 μl of Glycerol (100 v/v)
    200 μl of Sodium chloride (5 M)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    400 μl of Polyethylene glycol 600 (75 w/v)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    200 μl of water (100 v/v)
    F3
    • Zinc acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 10 v/v -
    		pH 6.5
    100 μl of 2-Propanol (100 v/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    133 μl of Zinc acetate (1.5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    666 μl of water (100 v/v)
    F4
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Glycerol 45 v/v -
    		pH 6.5
    450 μl of Glycerol (100 v/v)
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    200 μl of Calcium acetate (1 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    250 μl of water (100 v/v)
    F5
    • HEPES 0.1 M pH 7
    • Jeffamine® M-600 30 v/v -
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    600 μl of Jeffamine® M-600 (50 v/v)
    22 μl of HEPES pH 8 (1 M)
    300 μl of water (100 v/v)
    F6
    • Sodium di-hydrogen phosphate 0.1 M -
    • Sodium chloride 2 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    400 μl of Sodium chloride (5 M)
    33 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of MES pH 6.5 (1 M)
    25 μl of Potassium di-hydrogen phosphate (4 M)
    441 μl of water (100 v/v)
    F7
    • Zinc acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    		pH 6.5
    200 μl of Glycerol (100 v/v)
    38 μl of Sodium cacodylate pH 6.4 (1 M)
    106 μl of Zinc acetate (1.5 M)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    41 μl of Sodium cacodylate pH 6.6 (1 M)
    325 μl of water (100 v/v)
    F8
    • tri-Sodium citrate 0.1 M pH 5.5
    • 1,2-Propanediol 30 v/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    300 μl of 1,2-Propanediol (100 v/v)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    F9
    • Zinc acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of Zinc acetate (1.5 M)
    466 μl of water (100 v/v)
    F10
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 5 w/v -
    • 2-Propanol 35 v/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    350 μl of 2-Propanol (100 v/v)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    450 μl of water (100 v/v)
    F11
    • MES 0.1 M pH 6
    • Glycerol 10 v/v -
    • Polyethylene glycol 600 30 w/v -
    • Polyethylene glycol 1,000 5 w/v -
    		pH 6
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Glycerol (100 v/v)
    400 μl of Polyethylene glycol 600 (75 w/v)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    300 μl of water (100 v/v)
    F12
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    G1
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • 2-Propanol 35 v/v -
    		pH 8
    350 μl of 2-Propanol (100 v/v)
    133 μl of Zinc acetate (1.5 M)
    100 μl of Imidazole pH 8 (1 M)
    416 μl of water (100 v/v)
    G2
    • Potassium Sodium tartrate 1 M -
    • MES 0.1 M pH 6
    		pH 6
    500 μl of Potassium Sodium tartrate (2 M)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    400 μl of water (100 v/v)
    G3
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6
    • 1,4-Butanediol 20 v/v -
    		pH 6
    74 μl of Lithium sulfate (2.7 M)
    200 μl of 1,4-Butanediol (100 v/v)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    625 μl of water (100 v/v)
    G4
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6
    • Ethanol 15 v/v -
    		pH 6
    150 μl of Ethanol (100 v/v)
    133 μl of Zinc acetate (1.5 M)
    45 μl of MES pH 7 (1 M)
    54 μl of MES pH 5 (1 M)
    616 μl of water (100 v/v)
    G5
    • MES 0.1 M pH 5
    • Ammonium sulfate 1.6 M -
    		pH 5
    400 μl of Ammonium sulfate (4 M)
    100 μl of MES pH 5 (1 M)
    500 μl of water (100 v/v)
    G6
    • MES 0.1 M pH 5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 5
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 5 (1 M)
    300 μl of water (100 v/v)
    G7
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 300 40 v/v -
    		pH 8
    400 μl of Polyethylene glycol 300 (100 v/v)
    133 μl of Zinc acetate (1.5 M)
    100 μl of Imidazole pH 8 (1 M)
    366 μl of water (100 v/v)
    G8
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Ammonium acetate (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    G9
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    10 μl of Iron (III) chloride (1 M)
    200 μl of Jeffamine® M-600 (50 v/v)
    690 μl of water (100 v/v)
    G10
    • Ammonium di-hydrogen phosphate 0.7 M -
    • tri-Sodium citrate 0.07 M pH 5.6
    • Glycerol 30 v/v -
    		pH 5.6
    300 μl of Glycerol (100 v/v)
    280 μl of Ammonium di-hydrogen phosphate (2.5 M)
    70 μl of tri-Sodium citrate pH 5.6 (1 M)
    350 μl of water (100 v/v)
    G11
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Ethanol 15 v/v -
    		pH 5.5
    74 μl of Lithium sulfate (2.7 M)
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    150 μl of Ethanol (100 v/v)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    675 μl of water (100 v/v)
    G12
    • Calcium acetate 0.05 M -
    • Sodium acetate 0.1 M pH 4.5
    • 1,2-Propanediol 40 v/v -
    		pH 4.5
    50 μl of Calcium acetate (1 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    51 μl of Sodium acetate pH 4.4 (1 M)
    450 μl of water (100 v/v)
    H1
    • Sodium acetate 0.1 M pH 4.5
    • 2-Propanol 35 v/v -
    		pH 4.5
    350 μl of 2-Propanol (100 v/v)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    550 μl of water (100 v/v)
    H2
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    		pH 4.6
    200 μl of Ammonium acetate (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of water (100 v/v)
    H3
    • Ammonium acetate 0.17 M -
    • Sodium acetate 0.085 M pH 4.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		pH 4.6
    150 μl of Glycerol (100 v/v)
    170 μl of Ammonium acetate (1 M)
    85 μl of Sodium acetate pH 4.6 (1 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of water (100 v/v)
    H4
    • Zinc acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,000 20 w/v -
    		pH 4.5
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    133 μl of Zinc acetate (1.5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    366 μl of water (100 v/v)
    H5
    • di-Ammonium hydrogen phosphate 1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    614 μl of water (100 v/v)
    H6
    • Sodium di-hydrogen phosphate 0.8 M -
    • di-Potassium hydrogen phosphate 1.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    266 μl of Sodium di-hydrogen phosphate (3 M)
    300 μl of di-Potassium hydrogen phosphate (4 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    333 μl of water (100 v/v)
    H7
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Ammonium sulfate 0.2 M -
    • Ethylene glycol 40 v/v -
    		pH 4.2
    50 μl of Ammonium sulfate (4 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Ethylene glycol (100 v/v)
    450 μl of water (100 v/v)
    H8
    • Sodium chloride 1.5 M -
    • Ethanol 10 v/v -
    		NA
    100 μl of Ethanol (100 v/v)
    300 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    H9
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    		NA
    375 μl of Ammonium sulfate (4 M)
    250 μl of Glycerol (100 v/v)
    375 μl of water (100 v/v)
    H10
    • Sodium di-hydrogen phosphate 1.6 M -
    • di-Potassium hydrogen phosphate 0.4 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    		pH 4.2
    533 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of di-Potassium hydrogen phosphate (4 M)
    266 μl of water (100 v/v)
    H11
    • tri-Sodium citrate 0.1 M pH 2.5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 2.5
    24 μl of tri-Sodium citrate pH 1 (1 M)
    75 μl of tri-Sodium citrate pH 3 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    H12
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 30 w/v -
    		pH 4
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of water (100 v/v)