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Supplemental Information

Screen Description

Qiagen / JCSG Core IV
Created 2009-11-16.

Rigaku Recipe XML

μl
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Screen Structure

Well Count 96
Number of distinct ingredients 49
Internal diversity (Score mean dist)0.95
Internal diversity (Ion mean dist)0.93
Internal diversity (PEG mean dist)0.93
Internal diversity (Known pH mean dist)0.83
Internal diversity (Penalty mean dist)0.93

Screen Composition

Chemical classes bar chart
ingredient type JCSG Core IV
Buffer 116
Cryoprotectant 15
Organic (non-volatile) 41
Organic (volatile) 12
Polymer 38
Salt 104

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Core IV Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Ammonium sulfate 2 M -
  • Lithium sulfate 0.2 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
500.0 μl of Ammonium sulfate (4 M)
50.0 μl of CAPS pH 10.4 (1 M)
50.0 μl of CAPS pH 10.6 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
325.93 μl of water (100 v/v)
A2
  • Sodium di-hydrogen phosphate 1.2 M -
  • Potassium di-hydrogen phosphate 0.8 M -
  • Lithium sulfate 0.2 M -
  • Glycine 0.1 M pH 10.5
pH 10.5
100.0 μl of Glycine pH 10.5 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
400.0 μl of Sodium di-hydrogen phosphate (3 M)
225.93 μl of water (100 v/v)
A3
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • CAPS 0.1 M pH 10.5
pH 10.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CAPS pH 10.4 (1 M)
50.0 μl of CAPS pH 10.6 (1 M)
500.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 3,000 10 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
700.0 μl of water (100 v/v)
A5
  • Potassium Sodium tartrate 1 M -
  • Lithium sulfate 0.2 M -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Potassium Sodium tartrate (2 M)
325.93 μl of water (100 v/v)
A6
  • Polyethylene glycol 400 30 v/v -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
A7
  • Ethanol 15 v/v -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
150.0 μl of Ethanol (100 v/v)
750.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 300 40 v/v -
  • tri-Sodium citrate 0.2 M -
  • CHES 0.1 M pH 9.5
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
375.0 μl of water (100 v/v)
A9
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • CHES 0.1 M pH 9.5
pH 9.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
500.0 μl of water (100 v/v)
A10
  • Ammonium sulfate 1.6 M -
  • BICINE 0.1 M pH 9
pH 9
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
500.0 μl of water (100 v/v)
A11
  • Ammonium sulfate 0.8 M -
  • BICINE 0.1 M pH 9
pH 9
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
700.0 μl of water (100 v/v)
A12
  • Ammonium sulfate 2.4 M -
  • BICINE 0.1 M pH 9
pH 9
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 6,000 10 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
31.65 μl of BICINE pH 7.5 (1 M)
68.35 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
B2
  • Ammonium sulfate 2.4 M -
  • BICINE 0.1 M pH 9
pH 9
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 6,000 30 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
31.65 μl of BICINE pH 7.5 (1 M)
68.35 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
B4
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
31.65 μl of BICINE pH 7.5 (1 M)
68.35 μl of BICINE pH 9 (1 M)
250.0 μl of water (100 v/v)
B5
  • Magnesium chloride 2 M -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of water (100 v/v)
B6
  • 2-Propanol 10 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
B7
  • Ethylene glycol 50 v/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B8
  • 1,2-Propanediol 25 v/v -
  • Magnesium chloride 0.2 M -
  • Glycerol 10 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
470.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 4,000 30 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
74.07 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
225.93 μl of water (100 v/v)
B12
  • 2-Propanol 30 v/v -
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 1.5 M -
  • Glycerol 12 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
375.0 μl of Ammonium sulfate (4 M)
120.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
405.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 4,000 25.5 w/v -
  • Sodium acetate 0.17 M -
  • Glycerol 15 v/v -
  • tris chloride BCC 0.085 M pH 8.5
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
56.67 μl of Sodium acetate (3 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
198.33 μl of water (100 v/v)
C4
  • 2-Propanol 10 v/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
C5
  • Zinc acetate 0.2 M -
  • Sodium chloride 2.5 M -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium chloride (5 M)
133.33 μl of Zinc acetate (1.5 M)
266.67 μl of water (100 v/v)
C6
  • Sodium chloride 2.5 M -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
C8
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
pH 8
285.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
574.29 μl of water (100 v/v)
C9
  • Ammonium sulfate 1.6 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 6,000 5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
C11
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 6,000 10 w/v -
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
D1
  • Ammonium sulfate 3.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)
D2
  • Ammonium sulfate 1.26 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
315.0 μl of Ammonium sulfate (4 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
585.0 μl of water (100 v/v)
D3
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
510.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 200 50 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of water (100 v/v)
D5
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
555.56 μl of Lithium sulfate (2.7 M)
344.44 μl of water (100 v/v)
D6
  • Sodium chloride 4.3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)
D7
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 10,000 20 w/v -
  • Ethylene glycol 8 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
420.0 μl of water (100 v/v)
D9
  • tri-Sodium citrate 1.26 M -
  • Glycerol 10 v/v -
  • HEPES 0.09 M pH 7.5
pH 7.5
100.0 μl of Glycerol (100 v/v)
34.95 μl of HEPES pH 6.5 (1 M)
55.05 μl of HEPES pH 8 (1 M)
787.5 μl of tri-Sodium citrate (1.6 M)
22.5 μl of water (100 v/v)
D10
  • Ammonium sulfate 1.7 M -
  • Polyethylene glycol 400 1.7 v/v -
  • Glycerol 15 v/v -
  • HEPES 0.085 M pH 7.5
pH 7.5
425.0 μl of Ammonium sulfate (4 M)
150.0 μl of Glycerol (100 v/v)
33.01 μl of HEPES pH 6.5 (1 M)
51.99 μl of HEPES pH 8 (1 M)
17.0 μl of Polyethylene glycol 400 (100 v/v)
323.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 600 30 w/v -
  • Lithium sulfate 0.05 M -
  • Glycerol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Glycerol (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
18.52 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 600 (75 w/v)
381.48 μl of water (100 v/v)
D12
  • 1,2-Propanediol 30 v/v -
  • Polyethylene glycol 400 20 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
300.0 μl of 1,2-Propanediol (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E1
  • 1,2-Propanediol 25 v/v -
  • Ammonium sulfate 0.2 M -
  • Glycerol 10 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
250.0 μl of 1,2-Propanediol (100 v/v)
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
E2
  • Ethylene glycol 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
400.0 μl of Ethylene glycol (100 v/v)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of water (100 v/v)
E3
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
450.0 μl of water (100 v/v)
E4
  • Sodium formate 4 M -
NA
571.43 μl of Sodium formate (7 M)
428.57 μl of water (100 v/v)
E5
  • Sodium formate 3.6 M -
  • Glycerol 10 v/v -
NA
100.0 μl of Glycerol (100 v/v)
514.29 μl of Sodium formate (7 M)
385.71 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 40 v/v -
  • Calcium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Calcium acetate (1 M)
38.84 μl of HEPES pH 6.5 (1 M)
61.16 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 3,000 30 w/v -
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E8
  • Potassium Sodium tartrate 1 M -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
74.07 μl of Lithium sulfate (2.7 M)
500.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
325.93 μl of water (100 v/v)
E9
  • Polyethylene glycol 600 40 w/v -
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
200.0 μl of Calcium acetate (1 M)
533.33 μl of Polyethylene glycol 600 (75 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
166.67 μl of water (100 v/v)
E10
  • Ammonium sulfate 0.8 M -
  • HEPES 0.1 M pH 6.5
pH 6.5
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
E11
  • Ammonium sulfate 3.2 M -
  • HEPES 0.1 M pH 7
pH 7
800.0 μl of Ammonium sulfate (4 M)
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
100.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 6,000 30 w/v -
  • HEPES 0.1 M pH 6.5
pH 6.5
100.0 μl of HEPES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
F1
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
100.0 μl of Lithium chloride (10 M)
800.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 600 30 w/v -
  • Sodium chloride 1 M -
  • Glycerol 10 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
200.0 μl of Sodium chloride (5 M)
200.0 μl of water (100 v/v)
F3
  • Zinc acetate 0.2 M -
  • 2-Propanol 10 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
133.33 μl of Zinc acetate (1.5 M)
666.67 μl of water (100 v/v)
F4
  • Glycerol 45 v/v -
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
200.0 μl of Calcium acetate (1 M)
450.0 μl of Glycerol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
250.0 μl of water (100 v/v)
F5
  • Jeffamine® M-600 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
77.67 μl of HEPES pH 6.5 (1 M)
22.33 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
300.0 μl of water (100 v/v)
F6
  • Sodium chloride 2 M -
  • Sodium di-hydrogen phosphate 0.1 M -
  • Potassium di-hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
25.0 μl of Potassium di-hydrogen phosphate (4 M)
400.0 μl of Sodium chloride (5 M)
33.33 μl of Sodium di-hydrogen phosphate (3 M)
441.67 μl of water (100 v/v)
F7
  • Zinc acetate 0.16 M -
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
  • Sodium cacodylate 0.08 M pH 6.5
pH 6.5
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
38.48 μl of Sodium cacodylate pH 6.4 (1 M)
41.52 μl of Sodium cacodylate pH 6.6 (1 M)
106.67 μl of Zinc acetate (1.5 M)
325.33 μl of water (100 v/v)
F8
  • 1,2-Propanediol 30 v/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
300.0 μl of 1,2-Propanediol (100 v/v)
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
21.0 μl of tri-Sodium citrate pH 5.4 (1 M)
79.0 μl of tri-Sodium citrate pH 5.8 (1 M)
400.0 μl of water (100 v/v)
F9
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.33 μl of Zinc acetate (1.5 M)
466.67 μl of water (100 v/v)
F10
  • 2-Propanol 35 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
21.0 μl of tri-Sodium citrate pH 5.4 (1 M)
79.0 μl of tri-Sodium citrate pH 5.8 (1 M)
450.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 600 30 w/v -
  • Glycerol 10 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of Glycerol (100 v/v)
54.69 μl of MES pH 5 (1 M)
45.31 μl of MES pH 7 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
300.0 μl of water (100 v/v)
F12
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
21.0 μl of tri-Sodium citrate pH 5.4 (1 M)
79.0 μl of tri-Sodium citrate pH 5.8 (1 M)
500.0 μl of water (100 v/v)
G1
  • 2-Propanol 35 v/v -
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
pH 8
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
133.33 μl of Zinc acetate (1.5 M)
416.67 μl of water (100 v/v)
G2
  • Potassium Sodium tartrate 1 M -
  • MES 0.1 M pH 6
pH 6
54.69 μl of MES pH 5 (1 M)
45.31 μl of MES pH 7 (1 M)
500.0 μl of Potassium Sodium tartrate (2 M)
400.0 μl of water (100 v/v)
G3
  • 1,4-Butanediol 20 v/v -
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
pH 6
200.0 μl of 1,4-Butanediol (100 v/v)
74.07 μl of Lithium sulfate (2.7 M)
54.69 μl of MES pH 5 (1 M)
45.31 μl of MES pH 7 (1 M)
625.93 μl of water (100 v/v)
G4
  • Zinc acetate 0.2 M -
  • Ethanol 15 v/v -
  • MES 0.1 M pH 6
pH 6
150.0 μl of Ethanol (100 v/v)
54.69 μl of MES pH 5 (1 M)
45.31 μl of MES pH 7 (1 M)
133.33 μl of Zinc acetate (1.5 M)
616.67 μl of water (100 v/v)
G5
  • Ammonium sulfate 1.6 M -
  • MES 0.1 M pH 5
pH 5
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 5 (1 M)
500.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 6,000 30 w/v -
  • MES 0.1 M pH 5
pH 5
100.0 μl of MES pH 5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
G7
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 300 40 v/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
133.33 μl of Zinc acetate (1.5 M)
366.67 μl of water (100 v/v)
G8
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G9
  • Jeffamine® M-600 10 v/v -
  • Iron (III) chloride 0.01 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
10.0 μl of Iron (III) chloride (1 M)
200.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
G10
  • Glycerol 30 v/v -
  • Ammonium di-hydrogen phosphate 0.7 M -
  • tri-Sodium citrate 0.07 M pH 5.6
pH 5.6
280.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of tri-Sodium citrate pH 5.6 (1 M)
350.0 μl of water (100 v/v)
G11
  • Ethanol 15 v/v -
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
150.0 μl of Ethanol (100 v/v)
74.07 μl of Lithium sulfate (2.7 M)
21.0 μl of tri-Sodium citrate pH 5.4 (1 M)
79.0 μl of tri-Sodium citrate pH 5.8 (1 M)
675.93 μl of water (100 v/v)
G12
  • 1,2-Propanediol 40 v/v -
  • Calcium acetate 0.05 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
50.0 μl of Calcium acetate (1 M)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
450.0 μl of water (100 v/v)
H1
  • 2-Propanol 35 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
350.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
550.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium acetate 0.17 M -
  • Glycerol 15 v/v -
  • Sodium acetate 0.085 M pH 4.6
pH 4.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate pH 4.6 (1 M)
85.0 μl of water (100 v/v)
H4
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 1,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
133.33 μl of Zinc acetate (1.5 M)
366.67 μl of water (100 v/v)
H5
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
285.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
614.29 μl of water (100 v/v)
H6
  • di-Potassium hydrogen phosphate 1.2 M -
  • Sodium di-hydrogen phosphate 0.8 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
300.0 μl of di-Potassium hydrogen phosphate (4 M)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
266.67 μl of Sodium di-hydrogen phosphate (3 M)
333.33 μl of water (100 v/v)
H7
  • Ethylene glycol 40 v/v -
  • Ammonium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
50.0 μl of Ammonium sulfate (4 M)
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
450.0 μl of water (100 v/v)
H8
  • Sodium chloride 1.5 M -
  • Ethanol 10 v/v -
NA
100.0 μl of Ethanol (100 v/v)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
H9
  • Ammonium sulfate 1.5 M -
  • Glycerol 25 v/v -
NA
375.0 μl of Ammonium sulfate (4 M)
250.0 μl of Glycerol (100 v/v)
375.0 μl of water (100 v/v)
H10
  • Sodium di-hydrogen phosphate 1.6 M -
  • di-Potassium hydrogen phosphate 0.4 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
100.0 μl of di-Potassium hydrogen phosphate (4 M)
533.33 μl of Sodium di-hydrogen phosphate (3 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
266.67 μl of water (100 v/v)
H11
  • Polyethylene glycol 6,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 2.5
pH 2.5
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
95.11 μl of tri-Sodium citrate pH 1 (1 M)
4.89 μl of tri-Sodium citrate pH 3 (1 M)
300.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 6,000 30 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
58.0 μl of tri-Sodium citrate pH 3.5 (1 M)
42.0 μl of tri-Sodium citrate pH 5 (1 M)
200.0 μl of water (100 v/v)