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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG+
Barcodes
JCSG+
No barcodes found.
Screen Structure
JCSG+
Well Count | 96 |
---|---|
Number of distinct ingredients | 71 |
Internal diversity (score mean dist) | 0.962 |
Screen Composition
ingredient type | JCSG+ |
---|---|
Buffer | 102 |
Cryoprotectant | 6 |
Organic (non-volatile) | 25 |
Organic (volatile) | 6 |
Polymer | 65 |
Salt | 103 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 325.9 μl of water (100 v/v) |
A2 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 36.3 μl of tri-Sodium citrate pH 5.4 (1 M) 63.7 μl of tri-Sodium citrate pH 5.8 (1 M) 500.0 μl of water (100 v/v) |
A3 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 72.0 μl of tri-Ammonium citrate (2.5 M) 528.0 μl of water (100 v/v) |
A4 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 590.0 μl of water (100 v/v) |
A5 |
| NA | 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
A6 |
| pH 4.2 | 74.1 μl of Lithium sulfate (2.7 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 425.9 μl of water (100 v/v) |
A7 |
| pH 9.5 | 50.0 μl of CHES pH 9 (1 M) 50.0 μl of CHES pH 10 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
A8 |
| NA | 20.0 μl of Ammonium formate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
A9 |
| NA | 40.0 μl of Ammonium chloride (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v) |
A10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 14.3 μl of Potassium formate (14 M) 585.7 μl of water (100 v/v) |
A11 |
| pH 8.5 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57.1 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 342.9 μl of water (100 v/v) |
A12 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate (3 M) 533.3 μl of water (100 v/v) |
B1 |
| pH 4 | 200.0 μl of Ammonium sulfate (4 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
B2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
B3 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 80.0 μl of Ethylene glycol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v) |
B5 |
| pH 6.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 400.0 μl of water (100 v/v) |
B6 |
| pH 4.2 | 400.0 μl of Ethanol (100 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 400.0 μl of water (100 v/v) |
B7 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
B8 |
| pH 7 | 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 620.0 μl of water (100 v/v) |
B9 |
| pH 5 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
B10 |
| pH 6.5 | 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 320.0 μl of water (100 v/v) |
B11 |
| NA | 1000.0 μl of tri-Sodium citrate (1.6 M) |
B12 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Potassium citrate (2.5 M) 520.0 μl of water (100 v/v) |
C1 |
| pH 4.2 | 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 460.0 μl of water (100 v/v) |
C2 |
| pH 4 | 100.0 μl of Lithium chloride (10 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 400.0 μl of water (100 v/v) |
C3 |
| NA | 20.0 μl of Ammonium nitrate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
C4 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
C5 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 200.0 μl of Potassium di-hydrogen phosphate (4 M) 266.7 μl of Sodium di-hydrogen phosphate (3 M) 433.3 μl of water (100 v/v) |
C6 |
| pH 4.2 | 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 500.0 μl of water (100 v/v) |
C7 |
| pH 4.5 | 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 133.3 μl of Zinc acetate (1.5 M) 566.7 μl of water (100 v/v) |
C8 |
| pH 8.5 | 200.0 μl of Ethanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v) |
C9 |
| pH 6.2 | 250.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Glycerol (100 v/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 616.7 μl of water (100 v/v) |
C10 |
| pH 9 | 40.0 μl of 1,4-Dioxane (50 v/v) 100.0 μl of BICINE pH 9 (1 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 526.7 μl of water (100 v/v) |
C11 |
| pH 4.6 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of water (100 v/v) |
C12 |
| NA | 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
D1 |
| NA | 200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v) |
D3 |
| pH 6.2 | 500.0 μl of Polyethylene glycol 200 (100 v/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40.0 μl of Sodium chloride (5 M) 426.7 μl of water (100 v/v) |
D4 |
| pH 4.5 | 74.1 μl of Lithium sulfate (2.7 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 225.9 μl of water (100 v/v) |
D5 |
| pH 7.5 | 700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 200.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v) |
D7 |
| pH 8.5 | 74.1 μl of Lithium sulfate (2.7 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 425.9 μl of water (100 v/v) |
D8 |
| pH 8 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
D9 |
| NA | 42.5 μl of Ammonium sulfate (4 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 297.5 μl of water (100 v/v) |
D10 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 300.0 μl of water (100 v/v) |
D11 |
| pH 4.6 | 140.0 μl of 2-Propanol (100 v/v) 70.0 μl of Calcium chloride (2 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 420.0 μl of water (100 v/v) |
D12 |
| NA | 200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 10.0 μl of Potassium di-hydrogen phosphate (4 M) 470.0 μl of water (100 v/v) |
E1 |
| pH 6.5 | 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v) |
E2 |
| pH 6.5 | 500.0 μl of Ammonium sulfate (4 M) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 40.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v) |
E4 |
| pH 8.5 | 315.0 μl of Ammonium sulfate (4 M) 74.1 μl of Lithium sulfate (2.7 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 510.9 μl of water (100 v/v) |
E5 |
| pH 10 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of CAPS pH 10 (1 M) 50.0 μl of CAPS pH 11 (1 M) 500.0 μl of water (100 v/v) |
E6 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 133.3 μl of Zinc acetate (1.5 M) 366.7 μl of water (100 v/v) |
E7 |
| pH 6.5 | 100.0 μl of 2-Propanol (100 v/v) 48.1 μl of Sodium cacodylate pH 6.4 (1 M) 51.9 μl of Sodium cacodylate pH 6.6 (1 M) 133.3 μl of Zinc acetate (1.5 M) 666.7 μl of water (100 v/v) |
E8 |
| pH 4.5 | 48.1 μl of Sodium acetate pH 4.6 (1 M) 51.9 μl of Sodium acetate pH 4.4 (1 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 614.3 μl of water (100 v/v) |
E9 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Magnesium sulfate (2.5 M) 260.0 μl of water (100 v/v) |
E10 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
E11 |
| pH 6.5 | 160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 38.5 μl of Sodium cacodylate pH 6.4 (1 M) 41.5 μl of Sodium cacodylate pH 6.6 (1 M) 272.0 μl of water (100 v/v) |
E12 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v) |
F1 |
| pH 6.5 | 50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v) |
F2 |
| pH 5 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
F3 |
| pH 8.5 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v) |
F4 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 400.0 μl of Jeffamine® M-600 (50 v/v) 500.0 μl of water (100 v/v) |
F5 |
| pH 8.5 | 500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
F6 |
| pH 9 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of water (100 v/v) |
F7 |
| NA | 800.0 μl of di-Sodium succinate pH 7 (1 M) 200.0 μl of water (100 v/v) |
F8 |
| NA | 700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v) |
F9 |
| NA | 705.9 μl of di-Sodium malonate pH 7 (3.4 M) 294.1 μl of water (100 v/v) |
F10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 10.0 μl of Jeffamine® ED-2001 (50 v/v) 323.5 μl of di-Sodium malonate pH 7 (3.4 M) 566.5 μl of water (100 v/v) |
F11 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 833.3 μl of di-Sodium succinate (1.2 M) 46.7 μl of water (100 v/v) |
F12 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 300.0 μl of water (100 v/v) |
G1 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Jeffamine® ED-2001 (50 v/v) 300.0 μl of water (100 v/v) |
G2 |
| pH 7.5 | 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v) 452.0 μl of water (100 v/v) |
G3 |
| pH 8.5 | 10.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v) |
G4 |
| pH 8.5 | 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G5 |
| pH 7.5 | 5.0 μl of Cadmium chloride (1 M) 5.0 μl of Cobalt (II) chloride (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 2.0 μl of Magnesium chloride (2.5 M) 1.2 μl of Nickel (II) chloride (4 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 646.8 μl of water (100 v/v) |
G6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 70.6 μl of di-Sodium malonate pH 7 (3.4 M) 529.4 μl of water (100 v/v) |
G7 |
| NA | 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Sodium succinate pH 7 (1 M) 600.0 μl of water (100 v/v) |
G8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of di-Sodium DL-malate pH 7 (3 M) 550.0 μl of water (100 v/v) |
G9 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v) |
G10 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v) |
G11 |
| pH 5.5 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 400.0 μl of water (100 v/v) |
G12 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
H1 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Magnesium formate (1 M) 600.0 μl of water (100 v/v) |
H2 |
| pH 5.5 | 250.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 20.0 μl of Polyethylene glycol 3,350 (50 w/v) 630.0 μl of water (100 v/v) |
H3 |
| pH 5.5 | 90.9 μl of Ammonium acetate pH 4 (1 M) 9.1 μl of Ammonium acetate pH 6 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of Sodium acetate (3 M) 366.7 μl of water (100 v/v) |
H4 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Calcium chloride (2 M) 350.0 μl of water (100 v/v) |
H5 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 250.0 μl of water (100 v/v) |
H6 |
| pH 5.5 | 100.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 460.0 μl of water (100 v/v) |
H7 |
| pH 5.5 | 50.0 μl of Ammonium sulfate (4 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
H8 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
H9 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 74.1 μl of Lithium sulfate (2.7 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 325.9 μl of water (100 v/v) |
H10 |
| pH 5.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
H11 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
H12 |
| pH 7.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 38.8 μl of HEPES pH 6.5 (1 M) 61.2 μl of HEPES pH 8 (1 M) 250.0 μl of water (100 v/v) |