Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Jump to well contents.

Supplemental Information

Screen Description

JCSG+

Qiagen / JCSG+
Created 2009-11-16.

Export

JCSG+

Barcodes

JCSG+

    Screen Structure

    JCSG+

    Well Count 96
    Number of distinct ingredients 71
    Internal diversity (score mean dist) 0.962

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:43:08.704163 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG+
    Buffer 102
    Cryoprotectant 6
    Organic (non-volatile) 25
    Organic (volatile) 6
    Polymer 65
    Salt 103

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG+ Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 50 v/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    500 μl of Polyethylene glycol 400 (100 v/v)
    74 μl of Lithium sulfate (2.7 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    325 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    500 μl of water (100 v/v)
    A3
    • tri-Ammonium citrate 0.18 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    72 μl of tri-Ammonium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    528 μl of water (100 v/v)
    A4
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    10 μl of Calcium chloride (2 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    590 μl of water (100 v/v)
    A5
    • Magnesium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    A6
    • Lithium sulfate 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 20 w/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    425 μl of water (100 v/v)
    A7
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 9.5
    50 μl of CHES pH 10 (1 M)
    50 μl of CHES pH 9 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    A8
    • Ammonium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium formate (10 M)
    580 μl of water (100 v/v)
    A9
    • Ammonium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium chloride (5 M)
    560 μl of water (100 v/v)
    A10
    • Potassium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    14 μl of Potassium formate (14 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    585 μl of water (100 v/v)
    A11
    • di-Ammonium hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    		pH 8.5
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    342 μl of water (100 v/v)
    A12
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    66 μl of Potassium nitrate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    		pH 4
    200 μl of Ammonium sulfate (4 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    700 μl of water (100 v/v)
    B2
    • Sodium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate (8 M)
    575 μl of water (100 v/v)
    B3
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 20 w/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 8 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    620 μl of water (100 v/v)
    B5
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Polyethylene glycol 8,000 5 w/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    B6
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Ethanol 40 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of Ethanol (100 v/v)
    400 μl of water (100 v/v)
    B7
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    740 μl of water (100 v/v)
    B8
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 8,000 10 w/v -
    		pH 7
    80 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    620 μl of water (100 v/v)
    B9
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    B10
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 200 50 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    320 μl of water (100 v/v)
    B11
    • tri-Sodium citrate 1.6 M -
    		NA
    1000 μl of tri-Sodium citrate (1.6 M)
    B12
    • tri-Potassium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of tri-Potassium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    C1
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 8,000 20 w/v -
    		pH 4.2
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    C2
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of water (100 v/v)
    C3
    • Ammonium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium nitrate (10 M)
    580 μl of water (100 v/v)
    C4
    • Polyethylene glycol 6,000 10 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C5
    • Sodium di-hydrogen phosphate 0.8 M -
    • Potassium di-hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Potassium di-hydrogen phosphate (4 M)
    266 μl of Sodium di-hydrogen phosphate (3 M)
    433 μl of water (100 v/v)
    C6
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    		pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    500 μl of water (100 v/v)
    C7
    • Zinc acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,000 10 w/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    133 μl of Zinc acetate (1.5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    566 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ethanol (100 v/v)
    700 μl of water (100 v/v)
    C9
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    		pH 6.2
    100 μl of Glycerol (100 v/v)
    250 μl of 1,2-Propanediol (100 v/v)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    616 μl of water (100 v/v)
    C10
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 20,000 10 w/v -
    • 1,4-Dioxane 2 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    40 μl of 1,4-Dioxane (50 v/v)
    526 μl of water (100 v/v)
    C11
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    500 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    C12
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    600 μl of water (100 v/v)
    D1
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    D2
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    D3
    • Sodium chloride 0.2 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 200 50 v/v -
    		pH 6.2
    40 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    426 μl of water (100 v/v)
    D4
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 8,000 30 w/v -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    225 μl of water (100 v/v)
    D5
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 20 w/v -
    • Magnesium chloride 0.2 M -
    		pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    420 μl of water (100 v/v)
    D7
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 40 v/v -
    		pH 8.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    425 μl of water (100 v/v)
    D8
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    D9
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    		NA
    150 μl of Glycerol (100 v/v)
    42 μl of Ammonium sulfate (4 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    297 μl of water (100 v/v)
    D10
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 300 40 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    D11
    • Calcium chloride 0.14 M -
    • Sodium acetate 0.07 M pH 4.6
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    		pH 4.6
    300 μl of Glycerol (100 v/v)
    70 μl of Calcium chloride (2 M)
    140 μl of 2-Propanol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    420 μl of water (100 v/v)
    D12
    • Potassium di-hydrogen phosphate 0.04 M -
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    10 μl of Potassium di-hydrogen phosphate (4 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    470 μl of water (100 v/v)
    E1
    • Sodium cacodylate 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    275 μl of water (100 v/v)
    E2
    • Sodium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    40 μl of Sodium chloride (5 M)
    500 μl of Ammonium sulfate (4 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    360 μl of water (100 v/v)
    E3
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    		pH 7.5
    40 μl of Sodium chloride (5 M)
    38 μl of HEPES pH 6.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    760 μl of water (100 v/v)
    E4
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.26 M -
    		pH 8.5
    315 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    510 μl of water (100 v/v)
    E5
    • CAPS 0.1 M pH 10
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 10
    50 μl of CAPS pH 10 (1 M)
    50 μl of CAPS pH 11 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    E6
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 3,000 20 w/v -
    		pH 8
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Imidazole pH 8 (1 M)
    133 μl of Zinc acetate (1.5 M)
    366 μl of water (100 v/v)
    E7
    • Zinc acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 10 v/v -
    		pH 6.5
    48 μl of Sodium cacodylate pH 6.4 (1 M)
    51 μl of Sodium cacodylate pH 6.6 (1 M)
    100 μl of 2-Propanol (100 v/v)
    133 μl of Zinc acetate (1.5 M)
    666 μl of water (100 v/v)
    E8
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 4.5
    51 μl of Sodium acetate pH 4.4 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    48 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    E9
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    		pH 6.5
    640 μl of Magnesium sulfate (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    260 μl of water (100 v/v)
    E10
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 10 w/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    E11
    • Calcium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    		pH 6.5
    38 μl of Sodium cacodylate pH 6.4 (1 M)
    200 μl of Glycerol (100 v/v)
    41 μl of Sodium cacodylate pH 6.6 (1 M)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    160 μl of Calcium acetate (1 M)
    272 μl of water (100 v/v)
    E12
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 8,000 10 w/v -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    700 μl of water (100 v/v)
    F1
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    		pH 6.5
    50 μl of Cesium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    600 μl of Jeffamine® M-600 (50 v/v)
    250 μl of water (100 v/v)
    F2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 3.2 M -
    		pH 5
    800 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 8.5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Jeffamine® M-600 (50 v/v)
    500 μl of water (100 v/v)
    F5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ethylene glycol 50 v/v -
    		pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ethylene glycol (100 v/v)
    320 μl of water (100 v/v)
    F6
    • BICINE 0.1 M pH 9
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    800 μl of water (100 v/v)
    F7
    • di-Sodium succinate 0.8 M -
    		NA
    800 μl of di-Sodium succinate pH 7 (1 M)
    200 μl of water (100 v/v)
    F8
    • di-Sodium DL-malate 2.1 M -
    		NA
    700 μl of di-Sodium DL-malate pH 7 (3 M)
    300 μl of water (100 v/v)
    F9
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    F10
    • Jeffamine® ED-2001 0.5 v/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 1.1 M -
    		pH 7
    323 μl of di-Sodium malonate pH 7 (3.4 M)
    77 μl of HEPES pH 6.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    10 μl of Jeffamine® ED-2001 (50 v/v)
    566 μl of water (100 v/v)
    F11
    • Polyethylene glycol monomethyl ether 2,000 1 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium succinate 1 M -
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    833 μl of di-Sodium succinate (1.2 M)
    22 μl of HEPES pH 8 (1 M)
    46 μl of water (100 v/v)
    F12
    • HEPES 0.1 M pH 7
    • Jeffamine® M-600 30 v/v -
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    600 μl of Jeffamine® M-600 (50 v/v)
    300 μl of water (100 v/v)
    G1
    • HEPES 0.1 M pH 7
    • Jeffamine® ED-2001 30 v/v -
    		pH 7
    77 μl of HEPES pH 6.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    600 μl of Jeffamine® ED-2001 (50 v/v)
    300 μl of water (100 v/v)
    G2
    • Magnesium chloride 0.02 M -
    • HEPES 0.1 M pH 7.5
    • Poly(acrylic acid sodium salt) 5,100 22 v/v -
    		pH 7.5
    440 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v)
    8 μl of Magnesium chloride (2.5 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    452 μl of water (100 v/v)
    G3
    • Cobalt (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyvinylpyrrolidone K15 20 w/v -
    		pH 8.5
    10 μl of Cobalt (II) chloride (1 M)
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    490 μl of water (100 v/v)
    G4
    • Trimethylamine N-oxide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    		pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Trimethylamine N-oxide (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G5
    • Cobalt (II) chloride 0.005 M -
    • Cadmium chloride 0.005 M -
    • Magnesium chloride 0.005 M -
    • Nickel (II) chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 12 w/v -
    		pH 7.5
    2 μl of Magnesium chloride (2.5 M)
    38 μl of HEPES pH 6.5 (1 M)
    5 μl of Cobalt (II) chloride (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    1 μl of Nickel (II) chloride (4 M)
    61 μl of HEPES pH 8 (1 M)
    5 μl of Cadmium chloride (1 M)
    646 μl of water (100 v/v)
    G6
    • di-Sodium malonate 0.24 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    70 μl of di-Sodium malonate pH 7 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    529 μl of water (100 v/v)
    G7
    • di-Sodium succinate 0.1 M -
    • Polyethylene glycol 3,350 15 w/v -
    		NA
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Sodium succinate pH 7 (1 M)
    600 μl of water (100 v/v)
    G8
    • di-Sodium DL-malate 0.15 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of di-Sodium DL-malate pH 7 (3 M)
    550 μl of water (100 v/v)
    G9
    • Potassium thiocyanate 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    12 μl of Potassium thiocyanate (8 M)
    387 μl of water (100 v/v)
    G10
    • Potassium bromide 0.15 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    		NA
    37 μl of Potassium bromide (4 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    362 μl of water (100 v/v)
    G11
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    G12
    • BIS-TRIS 0.1 M pH 5.5
    • Sodium chloride 3 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H1
    • BIS-TRIS 0.1 M pH 5.5
    • Magnesium formate 0.3 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    300 μl of Magnesium formate (1 M)
    600 μl of water (100 v/v)
    H2
    • Polyethylene glycol 3,350 1 w/v -
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 1 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    250 μl of Ammonium sulfate (4 M)
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    630 μl of water (100 v/v)
    H3
    • Ammonium acetate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium acetate 0.1 M -
    		pH 5.5
    33 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    0 μl of Ammonium acetate pH 6 (1 M)
    100 μl of Ammonium acetate pH 4 (1 M)
    366 μl of water (100 v/v)
    H4
    • Calcium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    100 μl of Calcium chloride (2 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    350 μl of water (100 v/v)
    H5
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    H6
    • Ammonium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 10,000 17 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Ammonium acetate (1 M)
    460 μl of water (100 v/v)
    H7
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    50 μl of Ammonium sulfate (4 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    350 μl of water (100 v/v)
    H8
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    40 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    360 μl of water (100 v/v)
    H9
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    325 μl of water (100 v/v)
    H10
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    H11
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    320 μl of water (100 v/v)
    H12
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    		pH 7.5
    200 μl of Ammonium acetate (1 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)