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Supplemental Information

Screen Description

JCSG+

Qiagen / JCSG+
Created 2009-11-16.

Export

Barcodes

JCSG+

No barcodes found.

Screen Structure

JCSG+

Well Count 96
Number of distinct ingredients 71
Internal diversity (score mean dist) 0.962

Screen Composition

2025-08-16T06:24:49.105096 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG+
Buffer 102
Cryoprotectant 6
Organic (non-volatile) 25
Organic (volatile) 6
Polymer 65
Salt 103

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG+ Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 50 v/v -
pH 4.5
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
325.9 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
500.0 μl of water (100 v/v)
A3
  • tri-Ammonium citrate 0.18 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
72.0 μl of tri-Ammonium citrate (2.5 M)
528.0 μl of water (100 v/v)
A4
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
A5
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A6
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 20 w/v -
pH 4.2
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
425.9 μl of water (100 v/v)
A7
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
pH 9.5
50.0 μl of CHES pH 9 (1 M)
50.0 μl of CHES pH 10 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A9
  • Ammonium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A10
  • Potassium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.3 μl of Potassium formate (14 M)
585.7 μl of water (100 v/v)
A11
  • di-Ammonium hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
A12
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate (3 M)
533.3 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 0.8 M -
pH 4
200.0 μl of Ammonium sulfate (4 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
B2
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B3
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 20 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
B5
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
400.0 μl of water (100 v/v)
B6
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Ethanol 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 4.2
400.0 μl of Ethanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
B7
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
B8
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 8,000 10 w/v -
pH 7
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
620.0 μl of water (100 v/v)
B9
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
B10
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 200 50 v/v -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
320.0 μl of water (100 v/v)
B11
  • tri-Sodium citrate 1.6 M -
NA
1000.0 μl of tri-Sodium citrate (1.6 M)
B12
  • tri-Potassium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate (2.5 M)
520.0 μl of water (100 v/v)
C1
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 8,000 20 w/v -
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
C2
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
400.0 μl of water (100 v/v)
C3
  • Ammonium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium nitrate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.1 M pH 7
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C5
  • Sodium di-hydrogen phosphate 0.8 M -
  • Potassium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
266.7 μl of Sodium di-hydrogen phosphate (3 M)
433.3 μl of water (100 v/v)
C6
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
C7
  • Zinc acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,000 10 w/v -
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
133.3 μl of Zinc acetate (1.5 M)
566.7 μl of water (100 v/v)
C8
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 20 v/v -
pH 8.5
200.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
C9
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 6.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
616.7 μl of water (100 v/v)
C10
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 20,000 10 w/v -
  • 1,4-Dioxane 2 v/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
333.3 μl of Polyethylene glycol 20,000 (30 w/v)
526.7 μl of water (100 v/v)
C11
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
D2
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D3
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 200 50 v/v -
pH 6.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
426.7 μl of water (100 v/v)
D4
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 8,000 30 w/v -
pH 4.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
225.9 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of water (100 v/v)
D6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D7
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 40 v/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
425.9 μl of water (100 v/v)
D8
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
D9
  • Ammonium sulfate 0.17 M -
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
NA
42.5 μl of Ammonium sulfate (4 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
297.5 μl of water (100 v/v)
D10
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
300.0 μl of water (100 v/v)
D11
  • Calcium chloride 0.14 M -
  • Sodium acetate 0.07 M pH 4.6
  • 2-Propanol 14 v/v -
  • Glycerol 30 v/v -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
70.0 μl of Calcium chloride (2 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
420.0 μl of water (100 v/v)
D12
  • Potassium di-hydrogen phosphate 0.04 M -
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
10.0 μl of Potassium di-hydrogen phosphate (4 M)
470.0 μl of water (100 v/v)
E1
  • Sodium cacodylate 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
E2
  • Sodium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
pH 6.5
500.0 μl of Ammonium sulfate (4 M)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
E3
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
E4
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.26 M -
pH 8.5
315.0 μl of Ammonium sulfate (4 M)
74.1 μl of Lithium sulfate (2.7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
510.9 μl of water (100 v/v)
E5
  • CAPS 0.1 M pH 10
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 10
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CAPS pH 10 (1 M)
50.0 μl of CAPS pH 11 (1 M)
500.0 μl of water (100 v/v)
E6
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 3,000 20 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
133.3 μl of Zinc acetate (1.5 M)
366.7 μl of water (100 v/v)
E7
  • Zinc acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 10 v/v -
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
48.1 μl of Sodium cacodylate pH 6.4 (1 M)
51.9 μl of Sodium cacodylate pH 6.6 (1 M)
133.3 μl of Zinc acetate (1.5 M)
666.7 μl of water (100 v/v)
E8
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen phosphate 1 M -
pH 4.5
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
614.3 μl of water (100 v/v)
E9
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
640.0 μl of Magnesium sulfate (2.5 M)
260.0 μl of water (100 v/v)
E10
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 10 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
E11
  • Calcium acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
38.5 μl of Sodium cacodylate pH 6.4 (1 M)
41.5 μl of Sodium cacodylate pH 6.6 (1 M)
272.0 μl of water (100 v/v)
E12
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 8,000 10 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
F1
  • Cesium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Jeffamine® M-600 30 v/v -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
F2
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 3.2 M -
pH 5
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
F3
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 8.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7.5
  • Jeffamine® M-600 20 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Jeffamine® M-600 (50 v/v)
500.0 μl of water (100 v/v)
F5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ethylene glycol 50 v/v -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
F6
  • BICINE 0.1 M pH 9
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 9
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of water (100 v/v)
F7
  • di-Sodium succinate 0.8 M -
NA
800.0 μl of di-Sodium succinate pH 7 (1 M)
200.0 μl of water (100 v/v)
F8
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate pH 7 (3 M)
300.0 μl of water (100 v/v)
F9
  • di-Sodium malonate 2.4 M -
NA
705.9 μl of di-Sodium malonate pH 7 (3.4 M)
294.1 μl of water (100 v/v)
F10
  • Jeffamine® ED-2001 0.5 v/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 1.1 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
10.0 μl of Jeffamine® ED-2001 (50 v/v)
323.5 μl of di-Sodium malonate pH 7 (3.4 M)
566.5 μl of water (100 v/v)
F11
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium succinate 1 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
833.3 μl of di-Sodium succinate (1.2 M)
46.7 μl of water (100 v/v)
F12
  • HEPES 0.1 M pH 7
  • Jeffamine® M-600 30 v/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
300.0 μl of water (100 v/v)
G1
  • HEPES 0.1 M pH 7
  • Jeffamine® ED-2001 30 v/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
600.0 μl of Jeffamine® ED-2001 (50 v/v)
300.0 μl of water (100 v/v)
G2
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
  • Poly(acrylic acid sodium salt) 5,100 22 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 v/v)
452.0 μl of water (100 v/v)
G3
  • Cobalt (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyvinylpyrrolidone K15 20 w/v -
pH 8.5
10.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
G4
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G5
  • Cobalt (II) chloride 0.005 M -
  • Cadmium chloride 0.005 M -
  • Magnesium chloride 0.005 M -
  • Nickel (II) chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 12 w/v -
pH 7.5
5.0 μl of Cadmium chloride (1 M)
5.0 μl of Cobalt (II) chloride (1 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
2.0 μl of Magnesium chloride (2.5 M)
1.2 μl of Nickel (II) chloride (4 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
646.8 μl of water (100 v/v)
G6
  • di-Sodium malonate 0.24 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
70.6 μl of di-Sodium malonate pH 7 (3.4 M)
529.4 μl of water (100 v/v)
G7
  • di-Sodium succinate 0.1 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 7 (1 M)
600.0 μl of water (100 v/v)
G8
  • di-Sodium DL-malate 0.15 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
550.0 μl of water (100 v/v)
G9
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
G10
  • Potassium bromide 0.15 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
G11
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
400.0 μl of water (100 v/v)
G12
  • BIS-TRIS 0.1 M pH 5.5
  • Sodium chloride 3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
H1
  • BIS-TRIS 0.1 M pH 5.5
  • Magnesium formate 0.3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Magnesium formate (1 M)
600.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 3,350 1 w/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium sulfate 1 M -
pH 5.5
250.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
630.0 μl of water (100 v/v)
H3
  • Ammonium acetate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium acetate 0.1 M -
pH 5.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 6 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Sodium acetate (3 M)
366.7 μl of water (100 v/v)
H4
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H5
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
H6
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
H7
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
H8
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
H9
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
325.9 μl of water (100 v/v)
H10
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
H11
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
H12
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 7.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
250.0 μl of water (100 v/v)