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Supplemental Information

Screen Description

Mb Class Suite I

Qiagen / Mb Class Suite I
Created 2009-11-16.

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Mb Class Suite I

    Screen Structure

    Mb Class Suite I

    Well Count 96
    Number of distinct ingredients 53
    Internal diversity (score mean dist) 0.940

    Screen Composition

    2025-06-13T21:36:21.917740 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Mb Class Suite I
    Buffer 99
    Cryoprotectant 7
    Organic (non-volatile) 32
    Organic (volatile) 6
    Polymer 60
    Salt 122

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Mb Class Suite I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 10 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    62 μl of tri-Sodium citrate (1.6 M)
    100 μl of 2-Propanol (100 v/v)
    737 μl of water (100 v/v)
    A2
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 12 v/v -
    pH 4.6
    20 μl of Sodium chloride (5 M)
    120 μl of 2-Propanol (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    760 μl of water (100 v/v)
    A3
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 30 w/v -
    • Glycerol 20 w/v -
    pH 4.6
    300 μl of 2-Propanol (100 w/v)
    100 μl of Calcium chloride (2 M)
    200 μl of Glycerol (100 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.5 M -
    pH 8.5
    125 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    775 μl of water (100 v/v)
    A5
    • Sodium Potassium phosphate 0.1 M pH 7.5
    • Ammonium sulfate 0.7 M -
    pH 7.5
    175 μl of Ammonium sulfate (4 M)
    33 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    791 μl of water (100 v/v)
    A6
    • ADA 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    pH 6.5
    250 μl of Ammonium sulfate (4 M)
    200 μl of ADA pH 6.5 (0.5 M)
    550 μl of water (100 v/v)
    A7
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.2 M -
    pH 8.5
    300 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of water (100 v/v)
    A8
    • Ammonium acetate 0.1 M -
    • Ammonium sulfate 1.4 M -
    • 2-Propanol 4 w/v -
    NA
    350 μl of Ammonium sulfate (4 M)
    40 μl of 2-Propanol (100 w/v)
    100 μl of Ammonium acetate (1 M)
    510 μl of water (100 v/v)
    A9
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    A10
    • Sodium chloride 0.25 M -
    • Sodium Potassium phosphate 0.05 M pH 7.5
    • Ammonium sulfate 3.5 M -
    pH 7.5
    875 μl of Ammonium sulfate (4 M)
    50 μl of Sodium chloride (5 M)
    16 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    58 μl of water (100 v/v)
    A11
    • ADA 0.1 M pH 6.5
    • di-Ammonium hydrogen phosphate 1 M -
    pH 6.5
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    200 μl of ADA pH 6.5 (0.5 M)
    514 μl of water (100 v/v)
    A12
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • di-Sodium hydrogen phosphate 0.5 M -
    • di-Potassium hydrogen phosphate 0.5 M -
    pH 7.5
    25 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    125 μl of di-Potassium hydrogen phosphate (4 M)
    500 μl of di-Sodium hydrogen phosphate (1 M)
    250 μl of water (100 v/v)
    B1
    • di-Ammonium hydrogen phosphate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium hydrogen phosphate 0.5 M -
    • di-Potassium hydrogen phosphate 0.5 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of di-Potassium hydrogen phosphate (4 M)
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    500 μl of di-Sodium hydrogen phosphate (1 M)
    246 μl of water (100 v/v)
    B2
    • Lithium chloride 0.5 M -
    • tri-Sodium citrate 1 M -
    NA
    625 μl of tri-Sodium citrate (1.6 M)
    50 μl of Lithium chloride (10 M)
    325 μl of water (100 v/v)
    B3
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    825 μl of water (100 v/v)
    B4
    • Potassium Sodium tartrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium sulfate 0.4 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Potassium Sodium tartrate (2 M)
    160 μl of Magnesium sulfate (2.5 M)
    690 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    40 μl of Magnesium chloride (2.5 M)
    820 μl of water (100 v/v)
    B6
    • Magnesium sulfate 0.6 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    240 μl of Magnesium sulfate (2.5 M)
    620 μl of water (100 v/v)
    B7
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 4.6
    20 μl of Sodium chloride (5 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    760 μl of water (100 v/v)
    B8
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of Sodium chloride (5 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    760 μl of water (100 v/v)
    B9
    • ADA 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 6.5
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of ADA pH 6.5 (0.5 M)
    680 μl of water (100 v/v)
    B10
    • tri-Sodium citrate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 7.5
    62 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    717 μl of water (100 v/v)
    B11
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    37 μl of Lithium sulfate (2.7 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    743 μl of water (100 v/v)
    B12
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    pH 8.5
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    760 μl of water (100 v/v)
    C1
    • BIS-TRIS propane 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 25 w/v -
    pH 7
    250 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
    55 μl of BIS-TRIS propane pH 7.5 (1 M)
    44 μl of BIS-TRIS propane pH 6.5 (1 M)
    650 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.3 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    pH 5.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
    300 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of water (100 v/v)
    C3
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 4 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    823 μl of water (100 v/v)
    C4
    • Lithium sulfate 0.3 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 4 v/v -
    pH 6.5
    40 μl of Polyethylene glycol 400 (100 v/v)
    111 μl of Lithium sulfate (2.7 M)
    200 μl of ADA pH 6.5 (0.5 M)
    648 μl of water (100 v/v)
    C5
    • Magnesium sulfate 0.6 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 4 v/v -
    pH 7.5
    40 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Magnesium sulfate (2.5 M)
    620 μl of water (100 v/v)
    C6
    • tri-Sodium citrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 5 v/v -
    pH 8.5
    62 μl of tri-Sodium citrate (1.6 M)
    50 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    787 μl of water (100 v/v)
    C7
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 15 w/v -
    • Glycerol 15 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of Polyethylene glycol 400 (100 w/v)
    100 μl of Calcium chloride (2 M)
    150 μl of Glycerol (100 w/v)
    500 μl of water (100 v/v)
    C8
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 18 v/v -
    pH 4.6
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    680 μl of water (100 v/v)
    C9
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 18 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of Sodium chloride (5 M)
    180 μl of Polyethylene glycol 400 (100 v/v)
    700 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 18 v/v -
    pH 7.5
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    680 μl of water (100 v/v)
    C11
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 18 v/v -
    pH 7.5
    25 μl of Ammonium sulfate (4 M)
    180 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    695 μl of water (100 v/v)
    C12
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 4.6
    • Polyethylene glycol 400 25 w/v -
    pH 4.6
    250 μl of Polyethylene glycol 400 (100 w/v)
    14 μl of Magnesium acetate (3.4 M)
    50 μl of Sodium acetate pH 4.6 (1 M)
    685 μl of water (100 v/v)
    D1
    • Sodium sulfate 0.05 M -
    • Lithium sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 30 w/v -
    pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    18 μl of Lithium sulfate (2.7 M)
    50 μl of Sodium sulfate (1 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    581 μl of water (100 v/v)
    D2
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 48 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of Polyethylene glycol 400 (100 w/v)
    100 μl of Calcium chloride (2 M)
    320 μl of water (100 v/v)
    D3
    • tris chloride BCC 0.01 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 20 w/v -
    pH 7.5
    200 μl of Polyethylene glycol monomethyl ether 550 (100 w/v)
    10 μl of tris chloride BCC pH 7.5 (1 M)
    790 μl of water (100 v/v)
    D4
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol monomethyl ether 550 30 w/v -
    pH 8.5
    300 μl of Polyethylene glycol monomethyl ether 550 (100 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    610 μl of water (100 v/v)
    D5
    • Polyethylene glycol 600 35 w/v -
    NA
    466 μl of Polyethylene glycol 600 (75 w/v)
    533 μl of water (100 v/v)
    D6
    • Sodium chloride 0.35 M -
    • TRICINE 0.1 M pH 8
    • Polyethylene glycol 1,000 28 w/v -
    • Glycerol 10 w/v -
    pH 8
    47 μl of TRICINE pH 8.5 (1 M)
    53 μl of TRICINE pH 7.5 (1 M)
    70 μl of Sodium chloride (5 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Glycerol (100 w/v)
    170 μl of water (100 v/v)
    D7
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 1,500 10 w/v -
    • Ethanol 5 w/v -
    NA
    20 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Ethanol (100 w/v)
    40 μl of Magnesium chloride (2.5 M)
    690 μl of water (100 v/v)
    D8
    • Polyethylene glycol 2,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 2,000 (50 w/v)
    900 μl of water (100 v/v)
    D9
    • Magnesium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 2,000 10 w/v -
    pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    550 μl of water (100 v/v)
    D10
    • tri-Sodium citrate 0.02 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 6.2
    • Polyethylene glycol 2,000 15 w/v -
    pH 6.2
    12 μl of tri-Sodium citrate (1.6 M)
    33 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    654 μl of water (100 v/v)
    D11
    • Sodium chloride 0.5 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 6.8
    • Polyethylene glycol 2,000 15 w/v -
    pH 6.8
    100 μl of Sodium chloride (5 M)
    11 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    21 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
    566 μl of water (100 v/v)
    D12
    • BIS-TRIS propane 0.02 M pH 7
    • Polyethylene glycol 2,000 15 w/v -
    pH 7
    11 μl of BIS-TRIS propane pH 7.5 (1 M)
    8 μl of BIS-TRIS propane pH 6.5 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    680 μl of water (100 v/v)
    E1
    • Magnesium chloride 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 2,000 15 w/v -
    pH 7.5
    50 μl of HEPES pH 7.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    610 μl of water (100 v/v)
    E2
    • Polyethylene glycol 2,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    700 μl of water (100 v/v)
    E3
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 2,000 15 w/v -
    NA
    10 μl of Lithium chloride (10 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    690 μl of water (100 v/v)
    E4
    • Magnesium nitrate 0.3 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 2,000 20 w/v -
    • 2-methyl-2,4-pentanediol BCC 2.17 w/v -
    pH 8
    21 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Magnesium nitrate (3 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    378 μl of water (100 v/v)
    E5
    • Magnesium chloride 0.3 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 2,000 25 w/v -
    • Glycerol 15 w/v -
    pH 9
    150 μl of Glycerol (100 w/v)
    120 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    130 μl of water (100 v/v)
    E6
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    E7
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Polyethylene glycol 200 3 w/v -
    • Glycerol 20 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Glycerol (100 w/v)
    30 μl of Polyethylene glycol 200 (100 w/v)
    470 μl of water (100 v/v)
    E8
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    pH 7.5
    50 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    610 μl of water (100 v/v)
    E9
    • Sodium chloride 0.15 M -
    • tri-Sodium citrate 0.05 M pH 5.6
    • Polyethylene glycol 3,350 10 w/v -
    pH 5.6
    50 μl of tri-Sodium citrate pH 5.6 (1 M)
    30 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    720 μl of water (100 v/v)
    E10
    • tris chloride BCC 0.05 M pH 7.5
    • Polyethylene glycol 4,000 2 w/v -
    pH 7.5
    40 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    910 μl of water (100 v/v)
    E11
    • Sodium chloride 0.1 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 4,000 5 w/v -
    • Glycerol 10 w/v -
    pH 6.5
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    50 μl of MES pH 6.5 (1 M)
    100 μl of Glycerol (100 w/v)
    730 μl of water (100 v/v)
    E12
    • Sodium di-hydrogen phosphate 0.05 M pH 6.7
    • Polyethylene glycol 4,000 5 w/v -
    pH 6.7
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    8 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    8 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
    883 μl of water (100 v/v)
    F1
    • Polyethylene glycol 4,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    900 μl of water (100 v/v)
    F2
    • Polyethylene glycol 4,000 5 w/v -
    • Potassium chloride 0.1 M -
    NA
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium chloride (4 M)
    875 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 10 w/v -
    pH 4.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    650 μl of water (100 v/v)
    F4
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    680 μl of water (100 v/v)
    F5
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    675 μl of water (100 v/v)
    F6
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (5 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    F7
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    37 μl of Lithium sulfate (2.7 M)
    200 μl of ADA pH 6.5 (0.5 M)
    523 μl of water (100 v/v)
    F8
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    • 2-Propanol 2 v/v -
    pH 6.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    37 μl of Lithium sulfate (2.7 M)
    20 μl of 2-Propanol (100 v/v)
    200 μl of ADA pH 6.5 (0.5 M)
    503 μl of water (100 v/v)
    F9
    • Sodium di-hydrogen phosphate 0.05 M pH 6.8
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.8
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    5 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    10 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
    743 μl of water (100 v/v)
    F10
    • Potassium chloride 0.5 M -
    • MOPS 0.05 M pH 7
    • Polyethylene glycol 4,000 12 w/v -
    • Glycerol 20 w/v -
    pH 7
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    125 μl of Potassium chloride (4 M)
    27 μl of MOPS pH 6.5 (1 M)
    200 μl of Glycerol (100 w/v)
    22 μl of MOPS pH 7.5 (1 M)
    385 μl of water (100 v/v)
    F11
    • Lithium chloride 0.1 M -
    • tris chloride BCC 0.01 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    10 μl of tris chloride BCC pH 7.5 (1 M)
    10 μl of Lithium chloride (10 M)
    680 μl of water (100 v/v)
    F12
    • Sodium chloride 0.5 M -
    • BIS-TRIS propane 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (5 M)
    56 μl of BIS-TRIS propane pH 6.5 (1 M)
    43 μl of BIS-TRIS propane pH 8.5 (1 M)
    400 μl of water (100 v/v)
    G1
    • Sodium chloride 0.5 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (5 M)
    33 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
    466 μl of water (100 v/v)
    G2
    • Zinc acetate 0.15 M -
    • Zinc chloride 0.05 M -
    • tris chloride BCC 0.05 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Zinc chloride (2 M)
    100 μl of Zinc acetate (1.5 M)
    425 μl of water (100 v/v)
    G3
    • TRICINE 0.05 M pH 8
    • Polyethylene glycol 4,000 22 w/v -
    pH 8
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    23 μl of TRICINE pH 8.5 (1 M)
    26 μl of TRICINE pH 7.5 (1 M)
    510 μl of water (100 v/v)
    G4
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 8.5
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (5 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    410 μl of water (100 v/v)
    G5
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    G6
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol monomethyl ether 5,000 10 w/v -
    pH 5.6
    200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    29 μl of Magnesium acetate (3.4 M)
    670 μl of water (100 v/v)
    G7
    • Magnesium sulfate 0.1 M -
    • Polyethylene glycol 6,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Magnesium sulfate (2.5 M)
    860 μl of water (100 v/v)
    G8
    • Zinc acetate 0.15 M -
    • tris chloride BCC 0.05 M pH 7.5
    • Polyethylene glycol 6,000 10 w/v -
    • Zinc chloride 0.05 M -
    pH 7.5
    50 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Zinc chloride (2 M)
    100 μl of Zinc acetate (1.5 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    625 μl of water (100 v/v)
    G9
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 6,000 12 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    37 μl of Lithium sulfate (2.7 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    623 μl of water (100 v/v)
    G10
    • Sodium chloride 0.15 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 12 w/v -
    pH 8.5
    30 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    630 μl of water (100 v/v)
    G11
    • di-Sodium succinate 0.05 M pH 6.5
    • Polyethylene glycol 6,000 15 w/v -
    pH 6.5
    100 μl of di-Sodium succinate pH 6.5 (0.5 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    G12
    • Potassium di-hydrogen phosphate 0.025 M -
    • Polyethylene glycol 8,000 12 w/v -
    • 2-methyl-2,4-pentanediol BCC 10 w/v -
    NA
    100 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    6 μl of Potassium di-hydrogen phosphate (4 M)
    653 μl of water (100 v/v)
    H1
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 10,000 8 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    160 μl of Polyethylene glycol 10,000 (50 w/v)
    29 μl of Magnesium acetate (3.4 M)
    710 μl of water (100 v/v)
    H2
    • di-Potassium hydrogen phosphate 0.05 M -
    NA
    12 μl of di-Potassium hydrogen phosphate (4 M)
    987 μl of water (100 v/v)
    H3
    • di-Potassium hydrogen phosphate 1.5 M -
    NA
    375 μl of di-Potassium hydrogen phosphate (4 M)
    625 μl of water (100 v/v)
    H4
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    37 μl of Lithium sulfate (2.7 M)
    50 μl of Potassium Sodium tartrate (2 M)
    813 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 0.1 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    33 μl of Sodium acetate (3 M)
    866 μl of water (100 v/v)
    H6
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.1 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of Sodium chloride (5 M)
    880 μl of water (100 v/v)
    H7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    pH 8.5
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    880 μl of water (100 v/v)
    H8
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 1.5 M -
    pH 4.6
    300 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of water (100 v/v)
    H9
    • Sodium formate 0.1 M -
    • Sodium chloride 2 M -
    NA
    14 μl of Sodium formate (7 M)
    400 μl of Sodium chloride (5 M)
    585 μl of water (100 v/v)
    H10
    • tri-Sodium citrate 0.1 M -
    NA
    62 μl of tri-Sodium citrate (1.6 M)
    937 μl of water (100 v/v)
    H11
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1 M -
    pH 7.5
    625 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    275 μl of water (100 v/v)
    H12
    • tri-Sodium citrate 1 M -
    NA
    625 μl of tri-Sodium citrate (1.6 M)
    375 μl of water (100 v/v)