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Supplemental Information

Screen Description

Mb Class Suite I

Qiagen / Mb Class Suite I
Created 2009-11-16.

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Mb Class Suite I

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Screen Structure

Mb Class Suite I

Well Count 96
Number of distinct ingredients 53
Internal diversity (score mean dist) 0.940

Screen Composition

2025-08-16T14:27:59.264169 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Mb Class Suite I
Buffer 99
Cryoprotectant 7
Organic (non-volatile) 32
Organic (volatile) 6
Polymer 60
Salt 122

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Mb Class Suite I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
737.5 μl of water (100 v/v)
A2
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 12 v/v -
pH 4.6
120.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
A3
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 30 w/v -
  • Glycerol 20 w/v -
pH 4.6
300.0 μl of 2-Propanol (100 w/v)
100.0 μl of Calcium chloride (2 M)
200.0 μl of Glycerol (100 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 0.5 M -
pH 8.5
125.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
775.0 μl of water (100 v/v)
A5
  • Sodium Potassium phosphate 0.1 M pH 7.5
  • Ammonium sulfate 0.7 M -
pH 7.5
175.0 μl of Ammonium sulfate (4 M)
33.3 μl of Sodium Potassium phosphate pH 7.5 (3 M)
791.7 μl of water (100 v/v)
A6
  • ADA 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
250.0 μl of Ammonium sulfate (4 M)
550.0 μl of water (100 v/v)
A7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.2 M -
pH 8.5
300.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
A8
  • Ammonium acetate 0.1 M -
  • Ammonium sulfate 1.4 M -
  • 2-Propanol 4 w/v -
NA
40.0 μl of 2-Propanol (100 w/v)
100.0 μl of Ammonium acetate (1 M)
350.0 μl of Ammonium sulfate (4 M)
510.0 μl of water (100 v/v)
A9
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
A10
  • Sodium chloride 0.25 M -
  • Sodium Potassium phosphate 0.05 M pH 7.5
  • Ammonium sulfate 3.5 M -
pH 7.5
875.0 μl of Ammonium sulfate (4 M)
16.7 μl of Sodium Potassium phosphate pH 7.5 (3 M)
50.0 μl of Sodium chloride (5 M)
58.3 μl of water (100 v/v)
A11
  • ADA 0.1 M pH 6.5
  • di-Ammonium hydrogen phosphate 1 M -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
514.3 μl of water (100 v/v)
A12
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • di-Sodium hydrogen phosphate 0.5 M -
  • di-Potassium hydrogen phosphate 0.5 M -
pH 7.5
25.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
125.0 μl of di-Potassium hydrogen phosphate (4 M)
500.0 μl of di-Sodium hydrogen phosphate (1 M)
250.0 μl of water (100 v/v)
B1
  • di-Ammonium hydrogen phosphate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • di-Sodium hydrogen phosphate 0.5 M -
  • di-Potassium hydrogen phosphate 0.5 M -
pH 8.5
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
125.0 μl of di-Potassium hydrogen phosphate (4 M)
500.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
246.4 μl of water (100 v/v)
B2
  • Lithium chloride 0.5 M -
  • tri-Sodium citrate 1 M -
NA
50.0 μl of Lithium chloride (10 M)
625.0 μl of tri-Sodium citrate (1.6 M)
325.0 μl of water (100 v/v)
B3
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
825.9 μl of water (100 v/v)
B4
  • Potassium Sodium tartrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium sulfate 0.4 M -
pH 8.5
160.0 μl of Magnesium sulfate (2.5 M)
50.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
690.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
pH 5.6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
820.0 μl of water (100 v/v)
B6
  • Magnesium sulfate 0.6 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
pH 7.5
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Magnesium sulfate (2.5 M)
620.0 μl of water (100 v/v)
B7
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 4.6
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
B8
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 5.6
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
760.0 μl of water (100 v/v)
B9
  • ADA 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 6.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of ADA pH 6.5 (0.5 M)
680.0 μl of water (100 v/v)
B10
  • tri-Sodium citrate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 7.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
717.5 μl of water (100 v/v)
B11
  • Lithium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 8.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
37.0 μl of Lithium sulfate (2.7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
743.0 μl of water (100 v/v)
B12
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
pH 8.5
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
760.0 μl of water (100 v/v)
C1
  • BIS-TRIS propane 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 25 w/v -
pH 7
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
44.1 μl of BIS-TRIS propane pH 6.5 (1 M)
55.9 μl of BIS-TRIS propane pH 7.5 (1 M)
650.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.3 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
300.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
C3
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 4 v/v -
pH 5.6
37.0 μl of Lithium sulfate (2.7 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
823.0 μl of water (100 v/v)
C4
  • Lithium sulfate 0.3 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 4 v/v -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
111.1 μl of Lithium sulfate (2.7 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
648.9 μl of water (100 v/v)
C5
  • Magnesium sulfate 0.6 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 4 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Magnesium sulfate (2.5 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
620.0 μl of water (100 v/v)
C6
  • tri-Sodium citrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 5 v/v -
pH 8.5
50.0 μl of Polyethylene glycol 400 (100 v/v)
62.5 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
787.5 μl of water (100 v/v)
C7
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 15 w/v -
  • Glycerol 15 w/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
150.0 μl of Glycerol (100 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
150.0 μl of Polyethylene glycol 400 (100 w/v)
500.0 μl of water (100 v/v)
C8
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 18 v/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
680.0 μl of water (100 v/v)
C9
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 18 v/v -
pH 5.6
180.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
700.0 μl of water (100 v/v)
C10
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 18 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
680.0 μl of water (100 v/v)
C11
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 18 v/v -
pH 7.5
25.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
695.0 μl of water (100 v/v)
C12
  • Magnesium acetate 0.05 M -
  • Sodium acetate 0.05 M pH 4.6
  • Polyethylene glycol 400 25 w/v -
pH 4.6
14.7 μl of Magnesium acetate (3.4 M)
250.0 μl of Polyethylene glycol 400 (100 w/v)
50.0 μl of Sodium acetate pH 4.6 (1 M)
685.3 μl of water (100 v/v)
D1
  • Sodium sulfate 0.05 M -
  • Lithium sulfate 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 400 30 w/v -
pH 8.5
18.5 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 w/v)
50.0 μl of Sodium sulfate (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
581.5 μl of water (100 v/v)
D2
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 48 w/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
480.0 μl of Polyethylene glycol 400 (100 w/v)
320.0 μl of water (100 v/v)
D3
  • tris chloride BCC 0.01 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 20 w/v -
pH 7.5
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
790.0 μl of water (100 v/v)
D4
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol monomethyl ether 550 30 w/v -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
610.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 600 35 w/v -
NA
466.7 μl of Polyethylene glycol 600 (75 w/v)
533.3 μl of water (100 v/v)
D6
  • Sodium chloride 0.35 M -
  • TRICINE 0.1 M pH 8
  • Polyethylene glycol 1,000 28 w/v -
  • Glycerol 10 w/v -
pH 8
100.0 μl of Glycerol (100 w/v)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
70.0 μl of Sodium chloride (5 M)
53.0 μl of TRICINE pH 7.5 (1 M)
47.0 μl of TRICINE pH 8.5 (1 M)
170.0 μl of water (100 v/v)
D7
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 1,500 10 w/v -
  • Ethanol 5 w/v -
NA
50.0 μl of Ethanol (100 w/v)
40.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 1,500 (50 w/v)
20.0 μl of Sodium chloride (5 M)
690.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 2,000 5 w/v -
NA
100.0 μl of Polyethylene glycol 2,000 (50 w/v)
900.0 μl of water (100 v/v)
D9
  • Magnesium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 2,000 10 w/v -
pH 8.5
200.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
D10
  • tri-Sodium citrate 0.02 M -
  • Sodium di-hydrogen phosphate 0.1 M pH 6.2
  • Polyethylene glycol 2,000 15 w/v -
pH 6.2
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
33.3 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
12.5 μl of tri-Sodium citrate (1.6 M)
654.2 μl of water (100 v/v)
D11
  • Sodium chloride 0.5 M -
  • Sodium di-hydrogen phosphate 0.1 M pH 6.8
  • Polyethylene glycol 2,000 15 w/v -
pH 6.8
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
21.8 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
11.5 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
566.7 μl of water (100 v/v)
D12
  • BIS-TRIS propane 0.02 M pH 7
  • Polyethylene glycol 2,000 15 w/v -
pH 7
8.8 μl of BIS-TRIS propane pH 6.5 (1 M)
11.2 μl of BIS-TRIS propane pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
680.0 μl of water (100 v/v)
E1
  • Magnesium chloride 0.1 M -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 2,000 15 w/v -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
610.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 2,000 15 w/v -
NA
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
700.0 μl of water (100 v/v)
E3
  • Lithium chloride 0.1 M -
  • Polyethylene glycol 2,000 15 w/v -
NA
10.0 μl of Lithium chloride (10 M)
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
690.0 μl of water (100 v/v)
E4
  • Magnesium nitrate 0.3 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 2,000 20 w/v -
  • 2-methyl-2,4-pentanediol BCC 2.17 w/v -
pH 8
21.7 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
100.0 μl of Magnesium nitrate (3 M)
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
378.3 μl of water (100 v/v)
E5
  • Magnesium chloride 0.3 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 2,000 25 w/v -
  • Glycerol 15 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
150.0 μl of Glycerol (100 w/v)
120.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 2,000 (50 w/v)
130.0 μl of water (100 v/v)
E6
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
E7
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
  • Polyethylene glycol 200 3 w/v -
  • Glycerol 20 w/v -
pH 5.6
200.0 μl of Glycerol (100 w/v)
30.0 μl of Polyethylene glycol 200 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
470.0 μl of water (100 v/v)
E8
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
pH 7.5
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
610.0 μl of water (100 v/v)
E9
  • Sodium chloride 0.15 M -
  • tri-Sodium citrate 0.05 M pH 5.6
  • Polyethylene glycol 3,350 10 w/v -
pH 5.6
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
30.0 μl of Sodium chloride (5 M)
50.0 μl of tri-Sodium citrate pH 5.6 (1 M)
720.0 μl of water (100 v/v)
E10
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol 4,000 2 w/v -
pH 7.5
40.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
910.0 μl of water (100 v/v)
E11
  • Sodium chloride 0.1 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 4,000 5 w/v -
  • Glycerol 10 w/v -
pH 6.5
100.0 μl of Glycerol (100 w/v)
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
730.0 μl of water (100 v/v)
E12
  • Sodium di-hydrogen phosphate 0.05 M pH 6.7
  • Polyethylene glycol 4,000 5 w/v -
pH 6.7
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
8.4 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
8.3 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
883.3 μl of water (100 v/v)
F1
  • Polyethylene glycol 4,000 5 w/v -
NA
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
900.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 4,000 5 w/v -
  • Potassium chloride 0.1 M -
NA
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
875.0 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 10 w/v -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
650.0 μl of water (100 v/v)
F4
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
F5
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
pH 7.5
25.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
675.0 μl of water (100 v/v)
F6
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 4,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
F7
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
523.0 μl of water (100 v/v)
F8
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
  • 2-Propanol 2 v/v -
pH 6.5
20.0 μl of 2-Propanol (100 v/v)
200.0 μl of ADA pH 6.5 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
503.0 μl of water (100 v/v)
F9
  • Sodium di-hydrogen phosphate 0.05 M pH 6.8
  • Polyethylene glycol 4,000 12 w/v -
pH 6.8
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.9 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
5.8 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
743.3 μl of water (100 v/v)
F10
  • Potassium chloride 0.5 M -
  • MOPS 0.05 M pH 7
  • Polyethylene glycol 4,000 12 w/v -
  • Glycerol 20 w/v -
pH 7
200.0 μl of Glycerol (100 w/v)
27.9 μl of MOPS pH 6.5 (1 M)
22.1 μl of MOPS pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
125.0 μl of Potassium chloride (4 M)
385.0 μl of water (100 v/v)
F11
  • Lithium chloride 0.1 M -
  • tris chloride BCC 0.01 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
10.0 μl of Lithium chloride (10 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
680.0 μl of water (100 v/v)
F12
  • Sodium chloride 0.5 M -
  • BIS-TRIS propane 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
56.8 μl of BIS-TRIS propane pH 6.5 (1 M)
43.2 μl of BIS-TRIS propane pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
G1
  • Sodium chloride 0.5 M -
  • Sodium di-hydrogen phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
33.3 μl of Sodium di-hydrogen phosphate pH 7 (3 M)
466.7 μl of water (100 v/v)
G2
  • Zinc acetate 0.15 M -
  • Zinc chloride 0.05 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Zinc acetate (1.5 M)
25.0 μl of Zinc chloride (2 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
425.0 μl of water (100 v/v)
G3
  • TRICINE 0.05 M pH 8
  • Polyethylene glycol 4,000 22 w/v -
pH 8
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
26.5 μl of TRICINE pH 7.5 (1 M)
23.5 μl of TRICINE pH 8.5 (1 M)
510.0 μl of water (100 v/v)
G4
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 4,000 22 w/v -
pH 8.5
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
410.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
G6
  • Magnesium acetate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol monomethyl ether 5,000 10 w/v -
pH 5.6
29.4 μl of Magnesium acetate (3.4 M)
200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
670.6 μl of water (100 v/v)
G7
  • Magnesium sulfate 0.1 M -
  • Polyethylene glycol 6,000 5 w/v -
NA
40.0 μl of Magnesium sulfate (2.5 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
860.0 μl of water (100 v/v)
G8
  • Zinc acetate 0.15 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol 6,000 10 w/v -
  • Zinc chloride 0.05 M -
pH 7.5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Zinc acetate (1.5 M)
25.0 μl of Zinc chloride (2 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
625.0 μl of water (100 v/v)
G9
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 6,000 12 w/v -
pH 5.6
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
623.0 μl of water (100 v/v)
G10
  • Sodium chloride 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
630.0 μl of water (100 v/v)
G11
  • di-Sodium succinate 0.05 M pH 6.5
  • Polyethylene glycol 6,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 6.5 (0.5 M)
600.0 μl of water (100 v/v)
G12
  • Potassium di-hydrogen phosphate 0.025 M -
  • Polyethylene glycol 8,000 12 w/v -
  • 2-methyl-2,4-pentanediol BCC 10 w/v -
NA
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
6.2 μl of Potassium di-hydrogen phosphate (4 M)
653.8 μl of water (100 v/v)
H1
  • Magnesium acetate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 10,000 8 w/v -
pH 5.6
29.4 μl of Magnesium acetate (3.4 M)
160.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
710.6 μl of water (100 v/v)
H2
  • di-Potassium hydrogen phosphate 0.05 M -
NA
12.5 μl of di-Potassium hydrogen phosphate (4 M)
987.5 μl of water (100 v/v)
H3
  • di-Potassium hydrogen phosphate 1.5 M -
NA
375.0 μl of di-Potassium hydrogen phosphate (4 M)
625.0 μl of water (100 v/v)
H4
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
37.0 μl of Lithium sulfate (2.7 M)
50.0 μl of Potassium Sodium tartrate (2 M)
813.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.1 M -
pH 8.5
33.3 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
866.7 μl of water (100 v/v)
H6
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.1 M -
pH 5.6
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
880.0 μl of water (100 v/v)
H7
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.1 M -
pH 8.5
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
880.0 μl of water (100 v/v)
H8
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 1.5 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
H9
  • Sodium formate 0.1 M -
  • Sodium chloride 2 M -
NA
400.0 μl of Sodium chloride (5 M)
14.3 μl of Sodium formate (7 M)
585.7 μl of water (100 v/v)
H10
  • tri-Sodium citrate 0.1 M -
NA
62.5 μl of tri-Sodium citrate (1.6 M)
937.5 μl of water (100 v/v)
H11
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
H12
  • tri-Sodium citrate 1 M -
NA
625.0 μl of tri-Sodium citrate (1.6 M)
375.0 μl of water (100 v/v)