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Supplemental Information

Screen Description

Mb Class Suite II

Qiagen / Mb Class Suite II
Created 2009-11-16.

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Mb Class Suite II

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Mb Class Suite II

    Screen Structure

    Mb Class Suite II

    Well Count 96
    Number of distinct ingredients 29
    Internal diversity (score mean dist) 0.880

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:31:27.250262 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Mb Class Suite II
    Buffer 100
    Organic (non-volatile) 28
    Polymer 70
    Salt 143

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Mb Class Suite II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    37 μl of Lithium sulfate (2.7 M)
    577 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    614 μl of water (100 v/v)
    A3
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium hydrogen phosphate 2 M -
    		pH 8.5
    571 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    A5
    • ADA 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    		pH 6.5
    250 μl of Ammonium sulfate (4 M)
    200 μl of ADA pH 6.5 (0.5 M)
    550 μl of water (100 v/v)
    A6
    • Ammonium sulfate 2 M -
    		NA
    500 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    A7
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    		pH 8.5
    500 μl of Ammonium sulfate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.1 M pH 5.5
    • Ammonium sulfate 2.5 M -
    		pH 5.5
    625 μl of Ammonium sulfate (4 M)
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    275 μl of water (100 v/v)
    A9
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 2.5 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    625 μl of Ammonium sulfate (4 M)
    275 μl of water (100 v/v)
    A10
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2.5 M -
    		pH 7.5
    625 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    275 μl of water (100 v/v)
    A11
    • Polyethylene glycol 400 2 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    		pH 7.5
    500 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    380 μl of water (100 v/v)
    A12
    • di-Ammonium hydrogen phosphate 1 M -
    		NA
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    714 μl of water (100 v/v)
    B1
    • di-Sodium hydrogen phosphate 0.5 M -
    • Ammonium sulfate 0.1 M -
    • di-Potassium hydrogen phosphate 0.5 M -
    		NA
    125 μl of di-Potassium hydrogen phosphate (4 M)
    25 μl of Ammonium sulfate (4 M)
    500 μl of di-Sodium hydrogen phosphate (1 M)
    350 μl of water (100 v/v)
    B2
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1.5 M -
    		pH 8.5
    555 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    344 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    555 μl of Lithium sulfate (2.7 M)
    344 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Magnesium sulfate (2.5 M)
    500 μl of water (100 v/v)
    B5
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium sulfate 1 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Magnesium sulfate (2.5 M)
    500 μl of water (100 v/v)
    B6
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Magnesium sulfate 1 M -
    		pH 6.5
    400 μl of Magnesium sulfate (2.5 M)
    200 μl of ADA pH 6.5 (0.5 M)
    37 μl of Lithium sulfate (2.7 M)
    363 μl of water (100 v/v)
    B7
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 30 v/v -
    		pH 5.5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    94 μl of tri-Sodium citrate pH 5.8 (1 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    580 μl of water (100 v/v)
    B8
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    B9
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    580 μl of water (100 v/v)
    B10
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 30 v/v -
    		pH 7
    44 μl of MOPS pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    55 μl of MOPS pH 6.5 (1 M)
    600 μl of water (100 v/v)
    B11
    • Sodium chloride 0.1 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 30 v/v -
    		pH 7
    44 μl of MOPS pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    55 μl of MOPS pH 6.5 (1 M)
    580 μl of water (100 v/v)
    B12
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    C1
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    580 μl of water (100 v/v)
    C2
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    580 μl of water (100 v/v)
    C3
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 400 30 v/v -
    		pH 3.5
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    543 μl of water (100 v/v)
    C4
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 30 v/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    543 μl of water (100 v/v)
    C5
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    543 μl of water (100 v/v)
    C6
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    543 μl of water (100 v/v)
    C7
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    543 μl of water (100 v/v)
    C8
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • CAPSO 0.1 M pH 9.5
    • Polyethylene glycol 400 30 v/v -
    		pH 9.5
    101 μl of CAPSO pH 9.4 (0.5 M)
    20 μl of Sodium chloride (5 M)
    98 μl of CAPSO pH 9.6 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    443 μl of water (100 v/v)
    C9
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 30 v/v -
    		pH 4.5
    48 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    540 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 30 v/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    540 μl of water (100 v/v)
    C11
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    540 μl of water (100 v/v)
    C12
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    40 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    540 μl of water (100 v/v)
    D1
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    540 μl of water (100 v/v)
    D2
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • CAPSO 0.1 M pH 9.5
    • Polyethylene glycol 400 30 v/v -
    		pH 9.5
    40 μl of Magnesium chloride (2.5 M)
    101 μl of CAPSO pH 9.4 (0.5 M)
    20 μl of Sodium chloride (5 M)
    98 μl of CAPSO pH 9.6 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    440 μl of water (100 v/v)
    D3
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    560 μl of water (100 v/v)
    D4
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    580 μl of water (100 v/v)
    D5
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    37 μl of Lithium sulfate (2.7 M)
    563 μl of water (100 v/v)
    D6
    • Lithium sulfate 0.3 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    		pH 6.5
    200 μl of ADA pH 6.5 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    111 μl of Lithium sulfate (2.7 M)
    388 μl of water (100 v/v)
    D7
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    40 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    560 μl of water (100 v/v)
    D8
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    		pH 7.5
    25 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    575 μl of water (100 v/v)
    D9
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    		pH 8.5
    125 μl of tri-Sodium citrate (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    475 μl of water (100 v/v)
    D10
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    640 μl of water (100 v/v)
    D11
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    660 μl of water (100 v/v)
    D12
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    640 μl of water (100 v/v)
    E1
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    44 μl of MOPS pH 7.5 (1 M)
    55 μl of MOPS pH 6.5 (1 M)
    660 μl of water (100 v/v)
    E2
    • Sodium chloride 0.1 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    44 μl of MOPS pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    55 μl of MOPS pH 6.5 (1 M)
    640 μl of water (100 v/v)
    E3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    660 μl of water (100 v/v)
    E4
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    640 μl of water (100 v/v)
    E5
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    640 μl of water (100 v/v)
    E6
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.5
    48 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    51 μl of Sodium acetate pH 4.4 (1 M)
    37 μl of Lithium sulfate (2.7 M)
    603 μl of water (100 v/v)
    E7
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    37 μl of Lithium sulfate (2.7 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    603 μl of water (100 v/v)
    E8
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    37 μl of Lithium sulfate (2.7 M)
    603 μl of water (100 v/v)
    E9
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    37 μl of Lithium sulfate (2.7 M)
    603 μl of water (100 v/v)
    E10
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    37 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    603 μl of water (100 v/v)
    E11
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • CAPSO 0.1 M pH 9.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 9.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    101 μl of CAPSO pH 9.4 (0.5 M)
    20 μl of Sodium chloride (5 M)
    98 μl of CAPSO pH 9.6 (0.5 M)
    37 μl of Lithium sulfate (2.7 M)
    503 μl of water (100 v/v)
    E12
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 3.5
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    F1
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    600 μl of water (100 v/v)
    F2
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    F3
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    F4
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of water (100 v/v)
    F5
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • CAPSO 0.1 M pH 9.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 9.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    101 μl of CAPSO pH 9.4 (0.5 M)
    20 μl of Sodium chloride (5 M)
    98 μl of CAPSO pH 9.6 (0.5 M)
    500 μl of water (100 v/v)
    F6
    • Zinc acetate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Zinc acetate (1.5 M)
    593 μl of water (100 v/v)
    F7
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    610 μl of water (100 v/v)
    F8
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    660 μl of water (100 v/v)
    F9
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    37 μl of Lithium sulfate (2.7 M)
    623 μl of water (100 v/v)
    F10
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    640 μl of water (100 v/v)
    F11
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 6.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of ADA pH 6.5 (0.5 M)
    37 μl of Lithium sulfate (2.7 M)
    523 μl of water (100 v/v)
    F12
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    640 μl of water (100 v/v)
    G1
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Ammonium sulfate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    635 μl of water (100 v/v)
    G2
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    580 μl of water (100 v/v)
    G3
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    		pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    585 μl of water (100 v/v)
    G4
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 12 w/v -
    		NA
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    710 μl of water (100 v/v)
    G5
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    640 μl of water (100 v/v)
    G6
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    620 μl of water (100 v/v)
    G7
    • Magnesium chloride 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 6,000 12 w/v -
    		pH 6.5
    40 μl of Magnesium chloride (2.5 M)
    200 μl of ADA pH 6.5 (0.5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    520 μl of water (100 v/v)
    G8
    • di-Ammonium hydrogen phosphate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 12 w/v -
    		pH 8.5
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    631 μl of water (100 v/v)
    G9
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 2 w/v -
    		NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    370 μl of Lithium sulfate (2.7 M)
    589 μl of water (100 v/v)
    G10
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    633 μl of water (100 v/v)
    G11
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    18 μl of Imidazole pH 8 (1 M)
    81 μl of Imidazole pH 6 (1 M)
    133 μl of Zinc acetate (1.5 M)
    566 μl of water (100 v/v)
    G12
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    500 μl of water (100 v/v)
    H1
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    		pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    H2
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Ammonium sulfate (4 M)
    750 μl of water (100 v/v)
    H3
    • Lithium sulfate 0.5 M -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    185 μl of Lithium sulfate (2.7 M)
    614 μl of water (100 v/v)
    H4
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 1.5 M -
    		pH 7.5
    375 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    525 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium di-hydrogen phosphate 1.5 M -
    		pH 8.5
    375 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    525 μl of water (100 v/v)
    H6
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Potassium Sodium tartrate (2 M)
    400 μl of water (100 v/v)
    H7
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    466 μl of Sodium acetate (3 M)
    433 μl of water (100 v/v)
    H8
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    		pH 7.5
    875 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of water (100 v/v)
    H9
    • Sodium formate 2 M -
    		NA
    285 μl of Sodium formate (7 M)
    714 μl of water (100 v/v)
    H10
    • Sodium formate 4 M -
    		NA
    571 μl of Sodium formate (7 M)
    428 μl of water (100 v/v)
    H11
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium di-hydrogen phosphate 1.5 M -
    		pH 5.5
    94 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of Sodium di-hydrogen phosphate (3 M)
    5 μl of tri-Sodium citrate pH 5.4 (1 M)
    400 μl of water (100 v/v)
    H12
    • MES 0.1 M pH 6.5
    • Sodium di-hydrogen phosphate 1.5 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    500 μl of Sodium di-hydrogen phosphate (3 M)
    400 μl of water (100 v/v)