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Supplemental Information

Screen Description

Mb Class Suite II

Qiagen / Mb Class Suite II
Created 2009-11-16.

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Mb Class Suite II

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Screen Structure

Mb Class Suite II

Well Count 96
Number of distinct ingredients 29
Internal diversity (score mean dist) 0.880

Screen Composition

2025-08-16T06:25:41.320405 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Mb Class Suite II
Buffer 100
Organic (non-volatile) 28
Polymer 70
Salt 143

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Mb Class Suite II Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Lithium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • di-Ammonium hydrogen phosphate 1 M -
pH 4.6
37.0 μl of Lithium sulfate (2.7 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
577.2 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.6
  • di-Ammonium hydrogen phosphate 1 M -
pH 5.6
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
614.3 μl of water (100 v/v)
A3
  • tris chloride BCC 0.1 M pH 8.5
  • di-Ammonium hydrogen phosphate 2 M -
pH 8.5
571.4 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
A5
  • ADA 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
250.0 μl of Ammonium sulfate (4 M)
550.0 μl of water (100 v/v)
A6
  • Ammonium sulfate 2 M -
NA
500.0 μl of Ammonium sulfate (4 M)
500.0 μl of water (100 v/v)
A7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.1 M pH 5.5
  • Ammonium sulfate 2.5 M -
pH 5.5
625.0 μl of Ammonium sulfate (4 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
275.0 μl of water (100 v/v)
A9
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 2.5 M -
pH 6.5
625.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 6.5 (1 M)
275.0 μl of water (100 v/v)
A10
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2.5 M -
pH 7.5
625.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
275.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 400 2 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
380.0 μl of water (100 v/v)
A12
  • di-Ammonium hydrogen phosphate 1 M -
NA
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
714.3 μl of water (100 v/v)
B1
  • di-Sodium hydrogen phosphate 0.5 M -
  • Ammonium sulfate 0.1 M -
  • di-Potassium hydrogen phosphate 0.5 M -
NA
25.0 μl of Ammonium sulfate (4 M)
125.0 μl of di-Potassium hydrogen phosphate (4 M)
500.0 μl of di-Sodium hydrogen phosphate (1 M)
350.0 μl of water (100 v/v)
B2
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1.5 M -
pH 8.5
555.6 μl of Lithium sulfate (2.7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
344.4 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
555.6 μl of Lithium sulfate (2.7 M)
344.4 μl of water (100 v/v)
B4
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium sulfate 1 M -
pH 4.6
400.0 μl of Magnesium sulfate (2.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.1 M pH 5.6
  • Magnesium sulfate 1 M -
pH 5.6
400.0 μl of Magnesium sulfate (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
500.0 μl of water (100 v/v)
B6
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Magnesium sulfate 1 M -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
400.0 μl of Magnesium sulfate (2.5 M)
363.0 μl of water (100 v/v)
B7
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 30 v/v -
pH 5.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
63.7 μl of tri-Sodium citrate pH 5.8 (1 M)
580.0 μl of water (100 v/v)
B8
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
B9
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
B10
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 400 30 v/v -
pH 7
55.9 μl of MOPS pH 6.5 (1 M)
44.1 μl of MOPS pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
B11
  • Sodium chloride 0.1 M -
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 400 30 v/v -
pH 7
55.9 μl of MOPS pH 6.5 (1 M)
44.1 μl of MOPS pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
B12
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
C1
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
C2
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
C3
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 400 30 v/v -
pH 3.5
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
543.0 μl of water (100 v/v)
C4
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 30 v/v -
pH 5.5
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
543.0 μl of water (100 v/v)
C5
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
37.0 μl of Lithium sulfate (2.7 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
543.0 μl of water (100 v/v)
C6
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
543.0 μl of water (100 v/v)
C7
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
543.0 μl of water (100 v/v)
C8
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 400 30 v/v -
pH 9.5
101.3 μl of CAPSO pH 9.4 (0.5 M)
98.7 μl of CAPSO pH 9.6 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
443.0 μl of water (100 v/v)
C9
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
pH 4.5
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
20.0 μl of Sodium chloride (5 M)
540.0 μl of water (100 v/v)
C10
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 30 v/v -
pH 5.5
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
540.0 μl of water (100 v/v)
C11
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
540.0 μl of water (100 v/v)
C12
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
540.0 μl of water (100 v/v)
D1
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
540.0 μl of water (100 v/v)
D2
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 400 30 v/v -
pH 9.5
101.3 μl of CAPSO pH 9.4 (0.5 M)
98.7 μl of CAPSO pH 9.6 (0.5 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
440.0 μl of water (100 v/v)
D3
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
560.0 μl of water (100 v/v)
D4
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 30 v/v -
pH 5.6
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
580.0 μl of water (100 v/v)
D5
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 30 v/v -
pH 5.6
37.0 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
563.0 μl of water (100 v/v)
D6
  • Lithium sulfate 0.3 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
111.1 μl of Lithium sulfate (2.7 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
388.9 μl of water (100 v/v)
D7
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
D8
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
25.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
575.0 μl of water (100 v/v)
D9
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
D10
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 12 w/v -
pH 5.5
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
640.0 μl of water (100 v/v)
D11
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
660.0 μl of water (100 v/v)
D12
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
E1
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 4,000 12 w/v -
pH 7
55.9 μl of MOPS pH 6.5 (1 M)
44.1 μl of MOPS pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
660.0 μl of water (100 v/v)
E2
  • Sodium chloride 0.1 M -
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 4,000 12 w/v -
pH 7
55.9 μl of MOPS pH 6.5 (1 M)
44.1 μl of MOPS pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
E3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
660.0 μl of water (100 v/v)
E4
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
E5
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
640.0 μl of water (100 v/v)
E6
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 12 w/v -
pH 4.5
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
48.1 μl of Sodium acetate pH 4.6 (1 M)
51.9 μl of Sodium acetate pH 4.4 (1 M)
20.0 μl of Sodium chloride (5 M)
603.0 μl of water (100 v/v)
E7
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 12 w/v -
pH 5.5
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
603.0 μl of water (100 v/v)
E8
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
37.0 μl of Lithium sulfate (2.7 M)
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
603.0 μl of water (100 v/v)
E9
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
603.0 μl of water (100 v/v)
E10
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
603.0 μl of water (100 v/v)
E11
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 4,000 12 w/v -
pH 9.5
101.3 μl of CAPSO pH 9.4 (0.5 M)
98.7 μl of CAPSO pH 9.6 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
503.0 μl of water (100 v/v)
E12
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 4,000 12 w/v -
pH 3.5
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
F1
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 12 w/v -
pH 5.5
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
600.0 μl of water (100 v/v)
F2
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
F3
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
F4
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F5
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 4,000 12 w/v -
pH 9.5
101.3 μl of CAPSO pH 9.4 (0.5 M)
98.7 μl of CAPSO pH 9.6 (0.5 M)
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
F6
  • Zinc acetate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
66.7 μl of Zinc acetate (1.5 M)
593.3 μl of water (100 v/v)
F7
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
610.0 μl of water (100 v/v)
F8
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
660.0 μl of water (100 v/v)
F9
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
623.0 μl of water (100 v/v)
F10
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
640.0 μl of water (100 v/v)
F11
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
37.0 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
523.0 μl of water (100 v/v)
F12
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
G1
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
25.0 μl of Ammonium sulfate (4 M)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
635.0 μl of water (100 v/v)
G2
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
G3
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
585.9 μl of water (100 v/v)
G4
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 12 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
710.0 μl of water (100 v/v)
G5
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
G6
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
620.0 μl of water (100 v/v)
G7
  • Magnesium chloride 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 6,000 12 w/v -
pH 6.5
200.0 μl of ADA pH 6.5 (0.5 M)
40.0 μl of Magnesium chloride (2.5 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
520.0 μl of water (100 v/v)
G8
  • di-Ammonium hydrogen phosphate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
631.4 μl of water (100 v/v)
G9
  • Lithium sulfate 1 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
370.4 μl of Lithium sulfate (2.7 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
589.6 μl of water (100 v/v)
G10
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
633.3 μl of water (100 v/v)
G11
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
81.7 μl of Imidazole pH 6 (1 M)
18.3 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
133.3 μl of Zinc acetate (1.5 M)
566.7 μl of water (100 v/v)
G12
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H1
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
H2
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
750.0 μl of water (100 v/v)
H3
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
185.2 μl of Lithium sulfate (2.7 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
614.8 μl of water (100 v/v)
H4
  • HEPES 0.1 M pH 7.5
  • Potassium di-hydrogen phosphate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
375.0 μl of Potassium di-hydrogen phosphate (4 M)
525.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium di-hydrogen phosphate 1.5 M -
pH 8.5
375.0 μl of Potassium di-hydrogen phosphate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
H6
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Potassium Sodium tartrate (2 M)
400.0 μl of water (100 v/v)
H7
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
466.7 μl of Sodium acetate (3 M)
433.3 μl of water (100 v/v)
H8
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
H9
  • Sodium formate 2 M -
NA
285.7 μl of Sodium formate (7 M)
714.3 μl of water (100 v/v)
H10
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
H11
  • tri-Sodium citrate 0.1 M pH 5.5
  • Sodium di-hydrogen phosphate 1.5 M -
pH 5.5
500.0 μl of Sodium di-hydrogen phosphate (3 M)
63.7 μl of tri-Sodium citrate pH 5.6 (1 M)
36.3 μl of tri-Sodium citrate pH 5.4 (1 M)
400.0 μl of water (100 v/v)
H12
  • MES 0.1 M pH 6.5
  • Sodium di-hydrogen phosphate 1.5 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Sodium di-hydrogen phosphate (3 M)
400.0 μl of water (100 v/v)