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Supplemental Information

Screen Description

PACT

Qiagen / PACT
Created 2009-11-16.

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PACT

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PACT

    Screen Structure

    PACT

    Well Count 96
    Number of distinct ingredients 30
    Internal diversity (score mean dist) 0.848

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:23:16.281636 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PACT
    Buffer 108
    Polymer 96
    Salt 78

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PACT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • SPG buffer 0.1 M pH 4
    • Polyethylene glycol 1,500 25 w/v -
    		pH 4
    100 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A2
    • SPG buffer 0.1 M pH 5
    • Polyethylene glycol 1,500 25 w/v -
    		pH 5
    100 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of SPG buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    A3
    • SPG buffer 0.1 M pH 6
    • Polyethylene glycol 1,500 25 w/v -
    		pH 6
    100 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of SPG buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    A4
    • SPG buffer 0.1 M pH 7
    • Polyethylene glycol 1,500 25 w/v -
    		pH 7
    0 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of SPG buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    A5
    • SPG buffer 0.1 M pH 8
    • Polyethylene glycol 1,500 25 w/v -
    		pH 8
    0 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of SPG buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    A6
    • SPG buffer 0.1 M pH 9
    • Polyethylene glycol 1,500 25 w/v -
    		pH 9
    0 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of SPG buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    A7
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Sodium chloride 0.2 M -
    		pH 5
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    460 μl of water (100 v/v)
    A8
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Ammonium chloride 0.2 M -
    		pH 5
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    460 μl of water (100 v/v)
    A9
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Lithium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Lithium chloride (10 M)
    100 μl of Sodium acetate pH 5 (1 M)
    480 μl of water (100 v/v)
    A10
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Magnesium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    420 μl of water (100 v/v)
    A11
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Calcium chloride 0.2 M -
    		pH 5
    100 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    400 μl of water (100 v/v)
    A12
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Zinc chloride 0.01 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    5 μl of Zinc chloride (2 M)
    495 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 4
    		pH 4
    100 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B2
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 5
    		pH 5
    100 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of MIB buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    B3
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 6
    		pH 6
    100 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of MIB buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    B4
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 7
    		pH 7
    0 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of MIB buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    B5
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 8
    		pH 8
    0 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of MIB buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    B6
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 9
    		pH 9
    0 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of MIB buffer pH 10 (1 M)
    400 μl of water (100 v/v)
    B7
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium chloride 0.2 M -
    		pH 6
    40 μl of Sodium chloride (5 M)
    42 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    460 μl of water (100 v/v)
    B8
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Ammonium chloride 0.2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    460 μl of water (100 v/v)
    B9
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Lithium chloride 0.2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Lithium chloride (10 M)
    57 μl of MES pH 6.5 (1 M)
    480 μl of water (100 v/v)
    B10
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Magnesium chloride 0.2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    420 μl of water (100 v/v)
    B11
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Calcium chloride 0.2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    100 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B12
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    • Zinc chloride 0.01 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    5 μl of Zinc chloride (2 M)
    495 μl of water (100 v/v)
    C1
    • PCB buffer 0.1 M pH 4
    • Polyethylene glycol 1,500 25 w/v -
    		pH 4
    100 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C2
    • PCB buffer 0.1 M pH 5
    • Polyethylene glycol 1,500 25 w/v -
    		pH 5
    100 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of PCB buffer pH 9.5 (1 M)
    400 μl of water (100 v/v)
    C3
    • PCB buffer 0.1 M pH 6
    • Polyethylene glycol 1,500 25 w/v -
    		pH 6
    100 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of PCB buffer pH 9.5 (1 M)
    400 μl of water (100 v/v)
    C4
    • PCB buffer 0.1 M pH 7
    • Polyethylene glycol 1,500 25 w/v -
    		pH 7
    0 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of PCB buffer pH 9.5 (1 M)
    400 μl of water (100 v/v)
    C5
    • PCB buffer 0.1 M pH 8
    • Polyethylene glycol 1,500 25 w/v -
    		pH 8
    0 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of PCB buffer pH 9.5 (1 M)
    400 μl of water (100 v/v)
    C6
    • PCB buffer 0.1 M pH 9
    • Polyethylene glycol 1,500 25 w/v -
    		pH 9
    0 μl of PCB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of PCB buffer pH 9.5 (1 M)
    400 μl of water (100 v/v)
    C7
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    77 μl of HEPES pH 6.5 (1 M)
    460 μl of water (100 v/v)
    C8
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Ammonium chloride 0.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    77 μl of HEPES pH 6.5 (1 M)
    460 μl of water (100 v/v)
    C9
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Lithium chloride 0.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Lithium chloride (10 M)
    77 μl of HEPES pH 6.5 (1 M)
    480 μl of water (100 v/v)
    C10
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    77 μl of HEPES pH 6.5 (1 M)
    420 μl of water (100 v/v)
    C11
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    100 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    77 μl of HEPES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    C12
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Zinc chloride 0.01 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    5 μl of Zinc chloride (2 M)
    77 μl of HEPES pH 6.5 (1 M)
    495 μl of water (100 v/v)
    D1
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 4
    		pH 4
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 4 (1 M)
    400 μl of water (100 v/v)
    D2
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 5
    		pH 5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 4 (1 M)
    0 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D3
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 6
    		pH 6
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 4 (1 M)
    0 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D4
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 7
    		pH 7
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of MMT buffer pH 4 (1 M)
    99 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 8
    		pH 8
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of MMT buffer pH 4 (1 M)
    99 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D6
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 9
    		pH 9
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    0 μl of MMT buffer pH 4 (1 M)
    99 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D7
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    D8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Ammonium chloride 0.2 M -
    		pH 8
    40 μl of Ammonium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Lithium chloride 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Lithium chloride (10 M)
    480 μl of water (100 v/v)
    D10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Magnesium chloride 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    420 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Calcium chloride 0.2 M -
    		pH 8
    100 μl of Calcium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    • Zinc chloride 0.01 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    5 μl of Zinc chloride (2 M)
    495 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    		NA
    250 μl of Sodium fluoride (0.8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    350 μl of water (100 v/v)
    E2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium bromide 0.2 M -
    		NA
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    E3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    		NA
    200 μl of Sodium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    E4
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    575 μl of water (100 v/v)
    E5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium nitrate (7 M)
    571 μl of water (100 v/v)
    E6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    		NA
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    		NA
    66 μl of Sodium acetate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    E8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    400 μl of water (100 v/v)
    E9
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    		NA
    100 μl of Potassium Sodium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium Potassium phosphate 0.2 M -
    		NA
    200 μl of Sodium Potassium phosphate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    E11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    		NA
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    E12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    		NA
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    541 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    250 μl of Sodium fluoride (0.8 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of water (100 v/v)
    F2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium bromide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    460 μl of water (100 v/v)
    F3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    200 μl of Sodium iodide (1 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    F4
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    475 μl of water (100 v/v)
    F5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    F6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    28 μl of Sodium formate (7 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    471 μl of water (100 v/v)
    F7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    66 μl of Sodium acetate (3 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    433 μl of water (100 v/v)
    F8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    300 μl of water (100 v/v)
    F9
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    100 μl of Potassium Sodium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    F10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium Potassium phosphate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    200 μl of Sodium Potassium phosphate (1 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    F11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    375 μl of water (100 v/v)
    F12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 6.5
    		pH 6.5
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    441 μl of water (100 v/v)
    G1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    250 μl of Sodium fluoride (0.8 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of water (100 v/v)
    G2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium bromide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    460 μl of water (100 v/v)
    G3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    200 μl of Sodium iodide (1 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    G4
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    475 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    G6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    28 μl of Sodium formate (7 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    471 μl of water (100 v/v)
    G7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    66 μl of Sodium acetate (3 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    433 μl of water (100 v/v)
    G8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    300 μl of water (100 v/v)
    G9
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    100 μl of Potassium Sodium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    G10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium Potassium phosphate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    200 μl of Sodium Potassium phosphate (1 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    G11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    375 μl of water (100 v/v)
    G12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    441 μl of water (100 v/v)
    H1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    250 μl of Sodium fluoride (0.8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    250 μl of water (100 v/v)
    H2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium bromide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    40 μl of Sodium bromide (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    460 μl of water (100 v/v)
    H3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    200 μl of Sodium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H4
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    475 μl of water (100 v/v)
    H5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    H6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    471 μl of water (100 v/v)
    H7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    66 μl of Sodium acetate (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    433 μl of water (100 v/v)
    H8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H9
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    100 μl of Potassium Sodium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium Potassium phosphate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    200 μl of Sodium Potassium phosphate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    375 μl of water (100 v/v)
    H12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 8.5
    		pH 8.5
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    441 μl of water (100 v/v)