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Supplemental Information

Screen Description

PEG Suite II

Qiagen / PEG Suite II
Created 2009-11-16.

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PEG Suite II

    Screen Structure

    PEG Suite II

    Well Count 96
    Number of distinct ingredients 35
    Internal diversity (score mean dist) 0.899

    Screen Composition

    2025-06-14T13:25:24.657881 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Suite II
    Buffer 88
    Cryoprotectant 2
    Organic (non-volatile) 15
    Organic (volatile) 9
    Polymer 97
    Salt 90

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Suite II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 15 v/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Calcium chloride (2 M)
    700 μl of water (100 v/v)
    A2
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 15 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    750 μl of water (100 v/v)
    A3
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 15 v/v -
    pH 7.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    670 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 15 v/v -
    pH 8.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    625 μl of water (100 v/v)
    A5
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 25 v/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Magnesium chloride (2.5 M)
    610 μl of water (100 v/v)
    A6
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 25 v/v -
    pH 8.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    575 μl of water (100 v/v)
    A7
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 v/v -
    pH 7.5
    280 μl of Polyethylene glycol 400 (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    100 μl of Calcium chloride (2 M)
    520 μl of water (100 v/v)
    A8
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Calcium chloride (2 M)
    550 μl of water (100 v/v)
    A9
    • Sodium acetate 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Sodium acetate (3 M)
    566 μl of water (100 v/v)
    A10
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Magnesium chloride (2.5 M)
    560 μl of water (100 v/v)
    A11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    520 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    475 μl of water (100 v/v)
    B1
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    580 μl of water (100 v/v)
    B2
    • Zinc sulfate 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    5 μl of Zinc sulfate (2 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    645 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 25 w/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    500 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of water (100 v/v)
    B4
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    B5
    • Polyethylene glycol 1,500 15 w/v -
    NA
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    700 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,500 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    500 μl of water (100 v/v)
    B7
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B8
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    2 μl of Nickel (II) chloride (4 M)
    497 μl of water (100 v/v)
    B9
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    NA
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    B10
    • Sodium acetate 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    33 μl of Sodium acetate (3 M)
    266 μl of water (100 v/v)
    B11
    • Sodium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    66 μl of Sodium acetate (3 M)
    433 μl of water (100 v/v)
    B12
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    225 μl of water (100 v/v)
    C1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 4 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    C2
    • Polyethylene glycol 4,000 8 w/v -
    NA
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    840 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    740 μl of water (100 v/v)
    C4
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    620 μl of water (100 v/v)
    C5
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    33 μl of Sodium acetate (3 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    626 μl of water (100 v/v)
    C6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    660 μl of water (100 v/v)
    C7
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    505 μl of water (100 v/v)
    C8
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    66 μl of Sodium acetate (3 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    513 μl of water (100 v/v)
    C9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C10
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 18 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    360 μl of Polyethylene glycol 4,000 (50 w/v)
    540 μl of water (100 v/v)
    C11
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    425 μl of water (100 v/v)
    C12
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Calcium chloride (2 M)
    400 μl of water (100 v/v)
    D1
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    33 μl of Sodium acetate (3 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    426 μl of water (100 v/v)
    D2
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    D3
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 4,000 25 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    320 μl of water (100 v/v)
    D4
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Calcium chloride (2 M)
    300 μl of water (100 v/v)
    D5
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    D6
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    260 μl of water (100 v/v)
    D7
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    D8
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Calcium chloride (2 M)
    200 μl of water (100 v/v)
    D9
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    225 μl of water (100 v/v)
    D10
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    66 μl of Sodium acetate (3 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    D11
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    D12
    • Polyethylene glycol 4,000 35 w/v -
    NA
    700 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    E1
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 8 w/v -
    • Lithium chloride 0.8 M -
    pH 8.5
    80 μl of Lithium chloride (10 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    660 μl of water (100 v/v)
    E2
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 20 v/v -
    NA
    200 μl of 2-Propanol (100 v/v)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    600 μl of water (100 v/v)
    E3
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 10 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of 2-Propanol (100 v/v)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    600 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 5 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    650 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 20 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    500 μl of water (100 v/v)
    E6
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Ammonium sulfate (4 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    610 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 15 w/v -
    NA
    50 μl of Ammonium sulfate (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    650 μl of water (100 v/v)
    E8
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Ammonium sulfate (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    550 μl of water (100 v/v)
    E9
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 16 w/v -
    • 2-Propanol 10 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    50 μl of Ammonium sulfate (4 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    430 μl of water (100 v/v)
    E10
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 20 w/v -
    NA
    50 μl of Ammonium sulfate (4 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    550 μl of water (100 v/v)
    E11
    • Magnesium sulfate 0.2 M -
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 10 v/v -
    NA
    80 μl of Magnesium sulfate (2.5 M)
    100 μl of Glycerol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    420 μl of water (100 v/v)
    E12
    • tri-Sodium citrate 0.1 M -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 5 v/v -
    NA
    50 μl of 2-Propanol (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    487 μl of water (100 v/v)
    F1
    • tri-Sodium citrate 0.1 M -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 v/v -
    NA
    200 μl of 2-Propanol (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    337 μl of water (100 v/v)
    F2
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    • Sodium chloride 0.6 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    120 μl of Sodium chloride (5 M)
    380 μl of water (100 v/v)
    F3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    F4
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 4,000 22 w/v -
    NA
    50 μl of Ammonium sulfate (4 M)
    33 μl of Sodium acetate (3 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    476 μl of water (100 v/v)
    F5
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Ammonium sulfate (4 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    350 μl of water (100 v/v)
    F6
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Ammonium sulfate (4 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    350 μl of water (100 v/v)
    F7
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 25 w/v -
    • Lithium sulfate 0.2 M -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    33 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    74 μl of Lithium sulfate (2.7 M)
    292 μl of water (100 v/v)
    F8
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 4,000 25 w/v -
    • 2-Propanol 8 v/v -
    NA
    80 μl of 2-Propanol (100 v/v)
    33 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    386 μl of water (100 v/v)
    F9
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    NA
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    350 μl of water (100 v/v)
    F10
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    250 μl of water (100 v/v)
    F11
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 5.6
    50 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 5.6 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    250 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium chloride 0.8 M -
    • Polyethylene glycol 4,000 32 w/v -
    pH 8.5
    80 μl of Lithium chloride (10 M)
    640 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    180 μl of water (100 v/v)
    G1
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    74 μl of Lithium sulfate (2.7 M)
    325 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    50 μl of Ammonium sulfate (4 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    250 μl of water (100 v/v)
    G3
    • Potassium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 3 w/v -
    pH 8.5
    25 μl of Potassium chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    60 μl of Polyethylene glycol 6,000 (50 w/v)
    815 μl of water (100 v/v)
    G4
    • Magnesium chloride 0.01 M -
    • Polyethylene glycol 6,000 10 w/v -
    NA
    4 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    796 μl of water (100 v/v)
    G5
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 12 w/v -
    NA
    400 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    360 μl of water (100 v/v)
    G6
    • Polyethylene glycol 6,000 15 w/v -
    • Glycerol 5 v/v -
    NA
    50 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    650 μl of water (100 v/v)
    G7
    • Potassium chloride 0.05 M -
    • Magnesium chloride 0.01 M -
    • Polyethylene glycol 6,000 15 w/v -
    NA
    4 μl of Magnesium chloride (2.5 M)
    12 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    683 μl of water (100 v/v)
    G8
    • tri-Sodium citrate 0.01 M -
    • Polyethylene glycol 6,000 16 w/v -
    NA
    6 μl of tri-Sodium citrate (1.6 M)
    320 μl of Polyethylene glycol 6,000 (50 w/v)
    673 μl of water (100 v/v)
    G9
    • Polyethylene glycol 6,000 20 w/v -
    • Imidazole 0.05 M pH 8
    pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    50 μl of Imidazole pH 8 (1 M)
    550 μl of water (100 v/v)
    G10
    • Lithium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 25 w/v -
    pH 7.5
    10 μl of Lithium chloride (10 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    500 μl of Polyethylene glycol 6,000 (50 w/v)
    390 μl of water (100 v/v)
    G11
    • Lithium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 28 w/v -
    pH 8.5
    50 μl of Lithium chloride (10 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of Polyethylene glycol 6,000 (50 w/v)
    290 μl of water (100 v/v)
    G12
    • Lithium chloride 1 M -
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 6,000 30 w/v -
    NA
    100 μl of Lithium chloride (10 M)
    33 μl of Sodium acetate (3 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    266 μl of water (100 v/v)
    H1
    • tri-Sodium citrate 0.01 M -
    • Polyethylene glycol 6,000 33 w/v -
    NA
    6 μl of tri-Sodium citrate (1.6 M)
    660 μl of Polyethylene glycol 6,000 (50 w/v)
    333 μl of water (100 v/v)
    H2
    • Lithium sulfate 0.5 M -
    • Polyethylene glycol 8,000 2 w/v -
    NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    185 μl of Lithium sulfate (2.7 M)
    774 μl of water (100 v/v)
    H3
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 2 w/v -
    NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    370 μl of Lithium sulfate (2.7 M)
    589 μl of water (100 v/v)
    H4
    • Polyethylene glycol 8,000 4 w/v -
    NA
    80 μl of Polyethylene glycol 8,000 (50 w/v)
    920 μl of water (100 v/v)
    H5
    • Lithium chloride 0.2 M -
    • Magnesium sulfate 0.05 M -
    • Polyethylene glycol 8,000 8 w/v -
    NA
    20 μl of Lithium chloride (10 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    20 μl of Magnesium sulfate (2.5 M)
    800 μl of water (100 v/v)
    H6
    • Polyethylene glycol 8,000 8 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    H7
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.5
    133 μl of Zinc acetate (1.5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    63 μl of Imidazole pH 6 (1 M)
    36 μl of Imidazole pH 7 (1 M)
    566 μl of water (100 v/v)
    H8
    • Calcium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    61 μl of HEPES pH 8 (1 M)
    200 μl of Calcium acetate (1 M)
    500 μl of water (100 v/v)
    H9
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    14 μl of Magnesium acetate (3.4 M)
    33 μl of Sodium acetate (3 M)
    752 μl of water (100 v/v)
    H10
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    58 μl of Magnesium acetate (3.4 M)
    741 μl of water (100 v/v)
    H11
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 10 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Ethylene glycol (100 v/v)
    61 μl of HEPES pH 8 (1 M)
    600 μl of water (100 v/v)
    H12
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)