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Supplemental Information

Screen Description

PEG Suite II

Qiagen / PEG Suite II
Created 2009-11-16.

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Barcodes

PEG Suite II

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Screen Structure

PEG Suite II

Well Count 96
Number of distinct ingredients 35
Internal diversity (score mean dist) 0.899

Screen Composition

2025-08-16T16:27:05.678169 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PEG Suite II
Buffer 88
Cryoprotectant 2
Organic (non-volatile) 15
Organic (volatile) 9
Polymer 97
Salt 90

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG Suite II Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 15 v/v -
pH 4.6
50.0 μl of Calcium chloride (2 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
A2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 15 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
750.0 μl of water (100 v/v)
A3
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 15 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
670.0 μl of water (100 v/v)
A4
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 15 v/v -
pH 8.5
150.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
A5
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 25 v/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
610.0 μl of water (100 v/v)
A6
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 25 v/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
575.9 μl of water (100 v/v)
A7
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
A8
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
pH 4.6
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate (3 M)
566.7 μl of water (100 v/v)
A10
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
A11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
A12
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
B1
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
B2
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
5.0 μl of Zinc sulfate (2 M)
645.0 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 25 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of water (100 v/v)
B4
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 1,500 15 w/v -
NA
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
700.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,500 20 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
B8
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
2.5 μl of Nickel (II) chloride (4 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
497.5 μl of water (100 v/v)
B9
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
B10
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
266.7 μl of water (100 v/v)
B11
  • Sodium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
433.3 μl of water (100 v/v)
B12
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,000 30 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
225.9 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 4 w/v -
pH 4.6
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
820.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 4,000 8 w/v -
NA
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
840.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
C4
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 10 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
620.0 μl of water (100 v/v)
C5
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
626.7 μl of water (100 v/v)
C6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
C7
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
505.9 μl of water (100 v/v)
C8
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
513.3 μl of water (100 v/v)
C9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C10
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 18 w/v -
pH 4.6
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
540.0 μl of water (100 v/v)
C11
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
425.9 μl of water (100 v/v)
C12
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D1
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 22 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
426.7 μl of water (100 v/v)
D2
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
D3
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 4,000 25 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
320.0 μl of water (100 v/v)
D4
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
100.0 μl of Calcium chloride (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
D6
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
260.0 μl of water (100 v/v)
D7
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
D8
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 30 w/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of water (100 v/v)
D9
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
225.9 μl of water (100 v/v)
D10
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
D11
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 4,000 35 w/v -
NA
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
E1
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 8 w/v -
  • Lithium chloride 0.8 M -
pH 8.5
80.0 μl of Lithium chloride (10 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 20 v/v -
NA
200.0 μl of 2-Propanol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
E3
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
600.0 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 5 v/v -
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
E5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 20 v/v -
pH 7.5
200.0 μl of 2-Propanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
E6
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
610.0 μl of water (100 v/v)
E7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 15 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
E8
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 15 w/v -
pH 5.6
50.0 μl of Ammonium sulfate (4 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
550.0 μl of water (100 v/v)
E9
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 16 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
50.0 μl of Ammonium sulfate (4 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
430.0 μl of water (100 v/v)
E10
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 20 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
550.0 μl of water (100 v/v)
E11
  • Magnesium sulfate 0.2 M -
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 10 v/v -
NA
100.0 μl of Glycerol (100 v/v)
80.0 μl of Magnesium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
420.0 μl of water (100 v/v)
E12
  • tri-Sodium citrate 0.1 M -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 5 v/v -
NA
50.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
62.5 μl of tri-Sodium citrate (1.6 M)
487.5 μl of water (100 v/v)
F1
  • tri-Sodium citrate 0.1 M -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
NA
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
62.5 μl of tri-Sodium citrate (1.6 M)
337.5 μl of water (100 v/v)
F2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
  • Sodium chloride 0.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
120.0 μl of Sodium chloride (5 M)
380.0 μl of water (100 v/v)
F3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
F4
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 4,000 22 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
476.7 μl of water (100 v/v)
F5
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
F6
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 25 w/v -
pH 5.6
50.0 μl of Ammonium sulfate (4 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
350.0 μl of water (100 v/v)
F7
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 25 w/v -
  • Lithium sulfate 0.2 M -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
292.6 μl of water (100 v/v)
F8
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 4,000 25 w/v -
  • 2-Propanol 8 v/v -
NA
80.0 μl of 2-Propanol (100 v/v)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
386.7 μl of water (100 v/v)
F9
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
350.0 μl of water (100 v/v)
F10
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
250.0 μl of water (100 v/v)
F11
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
pH 5.6
50.0 μl of Ammonium sulfate (4 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.6 (1 M)
250.0 μl of water (100 v/v)
F12
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium chloride 0.8 M -
  • Polyethylene glycol 4,000 32 w/v -
pH 8.5
80.0 μl of Lithium chloride (10 M)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
180.0 μl of water (100 v/v)
G1
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 25 w/v -
pH 8.5
74.1 μl of Lithium sulfate (2.7 M)
500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
325.9 μl of water (100 v/v)
G2
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
50.0 μl of Ammonium sulfate (4 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
250.0 μl of water (100 v/v)
G3
  • Potassium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 3 w/v -
pH 8.5
60.0 μl of Polyethylene glycol 6,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
815.0 μl of water (100 v/v)
G4
  • Magnesium chloride 0.01 M -
  • Polyethylene glycol 6,000 10 w/v -
NA
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
796.0 μl of water (100 v/v)
G5
  • Sodium chloride 2 M -
  • Polyethylene glycol 6,000 12 w/v -
NA
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 6,000 15 w/v -
  • Glycerol 5 v/v -
NA
50.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
G7
  • Potassium chloride 0.05 M -
  • Magnesium chloride 0.01 M -
  • Polyethylene glycol 6,000 15 w/v -
NA
4.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
12.5 μl of Potassium chloride (4 M)
683.5 μl of water (100 v/v)
G8
  • tri-Sodium citrate 0.01 M -
  • Polyethylene glycol 6,000 16 w/v -
NA
320.0 μl of Polyethylene glycol 6,000 (50 w/v)
6.2 μl of tri-Sodium citrate (1.6 M)
673.8 μl of water (100 v/v)
G9
  • Polyethylene glycol 6,000 20 w/v -
  • Imidazole 0.05 M pH 8
pH 8
50.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
550.0 μl of water (100 v/v)
G10
  • Lithium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 25 w/v -
pH 7.5
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
10.0 μl of Lithium chloride (10 M)
500.0 μl of Polyethylene glycol 6,000 (50 w/v)
390.0 μl of water (100 v/v)
G11
  • Lithium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 28 w/v -
pH 8.5
50.0 μl of Lithium chloride (10 M)
560.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
290.0 μl of water (100 v/v)
G12
  • Lithium chloride 1 M -
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 6,000 30 w/v -
NA
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
266.7 μl of water (100 v/v)
H1
  • tri-Sodium citrate 0.01 M -
  • Polyethylene glycol 6,000 33 w/v -
NA
660.0 μl of Polyethylene glycol 6,000 (50 w/v)
6.2 μl of tri-Sodium citrate (1.6 M)
333.8 μl of water (100 v/v)
H2
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
185.2 μl of Lithium sulfate (2.7 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
774.8 μl of water (100 v/v)
H3
  • Lithium sulfate 1 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
370.4 μl of Lithium sulfate (2.7 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
589.6 μl of water (100 v/v)
H4
  • Polyethylene glycol 8,000 4 w/v -
NA
80.0 μl of Polyethylene glycol 8,000 (50 w/v)
920.0 μl of water (100 v/v)
H5
  • Lithium chloride 0.2 M -
  • Magnesium sulfate 0.05 M -
  • Polyethylene glycol 8,000 8 w/v -
NA
20.0 μl of Lithium chloride (10 M)
20.0 μl of Magnesium sulfate (2.5 M)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
800.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 8,000 8 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
H7
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
63.5 μl of Imidazole pH 6 (1 M)
36.5 μl of Imidazole pH 7 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
133.3 μl of Zinc acetate (1.5 M)
566.7 μl of water (100 v/v)
H8
  • Calcium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
200.0 μl of Calcium acetate (1 M)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H9
  • Magnesium acetate 0.05 M -
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
14.7 μl of Magnesium acetate (3.4 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
752.0 μl of water (100 v/v)
H10
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
58.8 μl of Magnesium acetate (3.4 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
741.2 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Ethylene glycol (100 v/v)
38.8 μl of HEPES pH 6.5 (1 M)
61.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)