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Supplemental Information

Screen Description

Qiagen / pH Clear Suite I
Created 2009-11-16.

Rigaku Recipe XML

μl
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Screen Structure

Well Count 96
Number of distinct ingredients 10
Internal diversity (score mean dist) 0.856

Screen Composition

2025-10-22T00:23:00.024637 image/svg+xml Matplotlib v3.10.6, https://matplotlib.org/
ingredient type pH Clear Suite I
Buffer 96
Organic (non-volatile) 24
Polymer 24
Salt 80

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
pH Clear Suite I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.1 M pH 4
  • Sodium chloride 1 M -
pH 4
200.0 μl of Sodium chloride (5 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 1 M -
pH 5
200.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
A3
  • MES 0.1 M pH 6
  • Sodium chloride 1 M -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
200.0 μl of Sodium chloride (5 M)
700.0 μl of water (100 v/v)
A4
  • HEPES 0.1 M pH 7
  • Sodium chloride 1 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
200.0 μl of Sodium chloride (5 M)
700.0 μl of water (100 v/v)
A5
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 1 M -
pH 8
200.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
A6
  • BICINE 0.1 M pH 9
  • Sodium chloride 1 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Sodium chloride (5 M)
700.0 μl of water (100 v/v)
A7
  • tri-Sodium citrate 0.1 M pH 4
  • Sodium chloride 2 M -
pH 4
400.0 μl of Sodium chloride (5 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 2 M -
pH 5
400.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
A9
  • MES 0.1 M pH 6
  • Sodium chloride 2 M -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
A10
  • HEPES 0.1 M pH 7
  • Sodium chloride 2 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
A11
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 2 M -
pH 8
400.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
A12
  • BICINE 0.1 M pH 9
  • Sodium chloride 2 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 4
  • Sodium chloride 3 M -
pH 4
600.0 μl of Sodium chloride (5 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 3 M -
pH 5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
B3
  • MES 0.1 M pH 6
  • Sodium chloride 3 M -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7
  • Sodium chloride 3 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
B5
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 3 M -
pH 8
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
B6
  • BICINE 0.1 M pH 9
  • Sodium chloride 3 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.1 M pH 4
  • Sodium chloride 4 M -
pH 4
800.0 μl of Sodium chloride (5 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 4 M -
pH 5
800.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
B9
  • MES 0.1 M pH 6
  • Sodium chloride 4 M -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
800.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
B10
  • HEPES 0.1 M pH 7
  • Sodium chloride 4 M -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
800.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
B11
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 4 M -
pH 8
800.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)
B12
  • BICINE 0.1 M pH 9
  • Sodium chloride 4 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 5 w/v -
pH 4
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
800.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 5 w/v -
pH 5
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
800.0 μl of water (100 v/v)
C3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 5 w/v -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 5 w/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
C5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 5 w/v -
pH 8
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
800.0 μl of water (100 v/v)
C6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 5 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
C7
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 10 w/v -
pH 4
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
C8
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
C9
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 10 w/v -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C10
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 10 w/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C11
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 10 w/v -
pH 8
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
C12
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 10 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
D3
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 20 w/v -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 20 w/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 20 w/v -
pH 8
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
D6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 20 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
D7
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 30 w/v -
pH 4
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
D8
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 30 w/v -
pH 5
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
D9
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 30 w/v -
pH 6
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
D10
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 30 w/v -
pH 7
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 30 w/v -
pH 8
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D12
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 30 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
E1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 0.8 M -
pH 4
200.0 μl of Ammonium sulfate (4 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
E2
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 0.8 M -
pH 5
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
E3
  • MES 0.1 M pH 6
  • Ammonium sulfate 0.8 M -
pH 6
200.0 μl of Ammonium sulfate (4 M)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
700.0 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 0.8 M -
pH 7
200.0 μl of Ammonium sulfate (4 M)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
700.0 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 0.8 M -
pH 8
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
E6
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 0.8 M -
pH 9
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
700.0 μl of water (100 v/v)
E7
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 1.6 M -
pH 4
400.0 μl of Ammonium sulfate (4 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
E8
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 1.6 M -
pH 5
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
E9
  • MES 0.1 M pH 6
  • Ammonium sulfate 1.6 M -
pH 6
400.0 μl of Ammonium sulfate (4 M)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
500.0 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 1.6 M -
pH 7
400.0 μl of Ammonium sulfate (4 M)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
500.0 μl of water (100 v/v)
E11
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 1.6 M -
pH 8
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
E12
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 1.6 M -
pH 9
400.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
500.0 μl of water (100 v/v)
F1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 2.4 M -
pH 4
600.0 μl of Ammonium sulfate (4 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
F2
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 2.4 M -
pH 5
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
F3
  • MES 0.1 M pH 6
  • Ammonium sulfate 2.4 M -
pH 6
600.0 μl of Ammonium sulfate (4 M)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
300.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 2.4 M -
pH 7
600.0 μl of Ammonium sulfate (4 M)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
300.0 μl of water (100 v/v)
F5
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 2.4 M -
pH 8
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
F6
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2.4 M -
pH 9
600.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of water (100 v/v)
F7
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 3.2 M -
pH 4
800.0 μl of Ammonium sulfate (4 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
F8
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 3.2 M -
pH 5
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
F9
  • MES 0.1 M pH 6
  • Ammonium sulfate 3.2 M -
pH 6
800.0 μl of Ammonium sulfate (4 M)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
100.0 μl of water (100 v/v)
F10
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 3.2 M -
pH 7
800.0 μl of Ammonium sulfate (4 M)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
100.0 μl of water (100 v/v)
F11
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 3.2 M -
pH 8
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)
F12
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 3.2 M -
pH 9
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
100.0 μl of water (100 v/v)
G1
  • tri-Sodium citrate 0.1 M pH 4
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 4
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
800.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
800.0 μl of water (100 v/v)
G3
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 6
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
800.0 μl of water (100 v/v)
G4
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 7
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
800.0 μl of water (100 v/v)
G5
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 8
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
800.0 μl of water (100 v/v)
G6
  • BICINE 0.1 M pH 9
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 9
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of water (100 v/v)
G7
  • tri-Sodium citrate 0.1 M pH 4
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 4
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
G8
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
700.0 μl of water (100 v/v)
G9
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 6
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
700.0 μl of water (100 v/v)
G10
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 7
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
700.0 μl of water (100 v/v)
G11
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 8
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
G12
  • BICINE 0.1 M pH 9
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 9
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
700.0 μl of water (100 v/v)
H1
  • tri-Sodium citrate 0.1 M pH 4
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 4
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
H2
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
500.0 μl of water (100 v/v)
H3
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
500.0 μl of water (100 v/v)
H4
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 7
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
500.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
H6
  • BICINE 0.1 M pH 9
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 9
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
500.0 μl of water (100 v/v)
H7
  • tri-Sodium citrate 0.1 M pH 4
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 4
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5 (1 M)
250.0 μl of water (100 v/v)
H8
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
250.0 μl of water (100 v/v)
H9
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 6
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.5 μl of MES pH 6.5 (1 M)
42.5 μl of MES pH 5 (1 M)
250.0 μl of water (100 v/v)
H10
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 7
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
77.7 μl of HEPES pH 6.5 (1 M)
22.3 μl of HEPES pH 8 (1 M)
250.0 μl of water (100 v/v)
H11
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 8
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
250.0 μl of water (100 v/v)
H12
  • BICINE 0.1 M pH 9
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 9
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
250.0 μl of water (100 v/v)