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Supplemental Information

Screen Description

pH Clear Suite I

Qiagen / pH Clear Suite I
Created 2009-11-16.

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pH Clear Suite I

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pH Clear Suite I

    Screen Structure

    pH Clear Suite I

    Well Count 96
    Number of distinct ingredients 10
    Internal diversity (score mean dist) 0.856

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:22:49.251565 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type pH Clear Suite I
    Buffer 96
    Organic (non-volatile) 24
    Polymer 24
    Salt 80

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    pH Clear Suite I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 1 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 1 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    A3
    • MES 0.1 M pH 6
    • Sodium chloride 1 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    200 μl of Sodium chloride (5 M)
    57 μl of MES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7
    • Sodium chloride 1 M -
    		pH 7
    200 μl of Sodium chloride (5 M)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 1 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 9
    • Sodium chloride 1 M -
    		pH 9
    200 μl of Sodium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 2 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 2 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    A9
    • MES 0.1 M pH 6
    • Sodium chloride 2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Sodium chloride (5 M)
    57 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    A10
    • HEPES 0.1 M pH 7
    • Sodium chloride 2 M -
    		pH 7
    400 μl of Sodium chloride (5 M)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    A12
    • BICINE 0.1 M pH 9
    • Sodium chloride 2 M -
    		pH 9
    400 μl of Sodium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 3 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 3 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6
    • Sodium chloride 3 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    600 μl of Sodium chloride (5 M)
    57 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7
    • Sodium chloride 3 M -
    		pH 7
    600 μl of Sodium chloride (5 M)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 3 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    B6
    • BICINE 0.1 M pH 9
    • Sodium chloride 3 M -
    		pH 9
    600 μl of Sodium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)
    B7
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 4 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 4 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    B9
    • MES 0.1 M pH 6
    • Sodium chloride 4 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    800 μl of Sodium chloride (5 M)
    57 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    B10
    • HEPES 0.1 M pH 7
    • Sodium chloride 4 M -
    		pH 7
    800 μl of Sodium chloride (5 M)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    B11
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 4 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    B12
    • BICINE 0.1 M pH 9
    • Sodium chloride 4 M -
    		pH 9
    800 μl of Sodium chloride (5 M)
    100 μl of BICINE pH 9 (1 M)
    100 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 5 w/v -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    800 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 5 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    800 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 5 w/v -
    		pH 6
    42 μl of MES pH 5 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 5 w/v -
    		pH 7
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 5 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    800 μl of water (100 v/v)
    C6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 5 w/v -
    		pH 9
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    C7
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 10 w/v -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    700 μl of water (100 v/v)
    C8
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C9
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 10 w/v -
    		pH 6
    42 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    C10
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 10 w/v -
    		pH 7
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    C11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 10 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C12
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 10 w/v -
    		pH 9
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    500 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    D6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 20 w/v -
    		pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 30 w/v -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    300 μl of water (100 v/v)
    D8
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    D9
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 30 w/v -
    		pH 6
    42 μl of MES pH 5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    D10
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 30 w/v -
    		pH 7
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 30 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    D12
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 30 w/v -
    		pH 9
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)
    E1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    E2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 0.8 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    E3
    • MES 0.1 M pH 6
    • Ammonium sulfate 0.8 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    57 μl of MES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 0.8 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    200 μl of Ammonium sulfate (4 M)
    77 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    E5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.8 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    E6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 0.8 M -
    		pH 9
    200 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    E7
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 1.6 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    400 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    E8
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 1.6 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    E9
    • MES 0.1 M pH 6
    • Ammonium sulfate 1.6 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    400 μl of Ammonium sulfate (4 M)
    57 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1.6 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    400 μl of Ammonium sulfate (4 M)
    77 μl of HEPES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    E11
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1.6 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Ammonium sulfate (4 M)
    500 μl of water (100 v/v)
    E12
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 1.6 M -
    		pH 9
    400 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    F1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 2.4 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    600 μl of Ammonium sulfate (4 M)
    300 μl of water (100 v/v)
    F2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 2.4 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Ammonium sulfate (4 M)
    300 μl of water (100 v/v)
    F3
    • MES 0.1 M pH 6
    • Ammonium sulfate 2.4 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    600 μl of Ammonium sulfate (4 M)
    57 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2.4 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    600 μl of Ammonium sulfate (4 M)
    77 μl of HEPES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 2.4 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Ammonium sulfate (4 M)
    300 μl of water (100 v/v)
    F6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    		pH 9
    600 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    300 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 3.2 M -
    		pH 4
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    800 μl of Ammonium sulfate (4 M)
    100 μl of water (100 v/v)
    F8
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 3.2 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of Ammonium sulfate (4 M)
    100 μl of water (100 v/v)
    F9
    • MES 0.1 M pH 6
    • Ammonium sulfate 3.2 M -
    		pH 6
    42 μl of MES pH 5 (1 M)
    800 μl of Ammonium sulfate (4 M)
    57 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    F10
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 3.2 M -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    800 μl of Ammonium sulfate (4 M)
    77 μl of HEPES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    F11
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 3.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of Ammonium sulfate (4 M)
    100 μl of water (100 v/v)
    F12
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 3.2 M -
    		pH 9
    800 μl of Ammonium sulfate (4 M)
    100 μl of BICINE pH 9 (1 M)
    100 μl of water (100 v/v)
    G1
    • tri-Sodium citrate 0.1 M pH 4
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 4
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    800 μl of water (100 v/v)
    G2
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 5
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    800 μl of water (100 v/v)
    G3
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 6
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of MES pH 5 (1 M)
    57 μl of MES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    G4
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 7
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 8
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of water (100 v/v)
    G6
    • BICINE 0.1 M pH 9
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 9
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    G7
    • tri-Sodium citrate 0.1 M pH 4
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 4
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    700 μl of water (100 v/v)
    G8
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    700 μl of water (100 v/v)
    G9
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 6
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of MES pH 5 (1 M)
    57 μl of MES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    G10
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 7
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    G11
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 8
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    700 μl of water (100 v/v)
    G12
    • BICINE 0.1 M pH 9
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    		pH 9
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    H1
    • tri-Sodium citrate 0.1 M pH 4
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 4
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    500 μl of water (100 v/v)
    H2
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    500 μl of water (100 v/v)
    H3
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 6
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of MES pH 5 (1 M)
    57 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    H4
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 7
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 8
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    H6
    • BICINE 0.1 M pH 9
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 9
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    H7
    • tri-Sodium citrate 0.1 M pH 4
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 4
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    250 μl of water (100 v/v)
    H8
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 5
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    250 μl of water (100 v/v)
    H9
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 6
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of MES pH 5 (1 M)
    57 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    H10
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 7
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 8
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    250 μl of water (100 v/v)
    H12
    • BICINE 0.1 M pH 9
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 9
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    250 μl of water (100 v/v)