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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Qiagen / pH Clear Suite I
Created 2009-11-16.
Rigaku Recipe XML
Export
Barcodes
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Screen Structure
Well Count | 96 |
---|---|
Number of distinct ingredients | 10 |
Internal diversity (score mean dist) | 0.856 |
Screen Composition
ingredient type | pH Clear Suite I |
---|---|
Buffer | 96 |
Organic (non-volatile) | 24 |
Polymer | 24 |
Salt | 80 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4 | 200.0 μl of Sodium chloride (5 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
A2 |
| pH 5 | 200.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
A3 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 200.0 μl of Sodium chloride (5 M) 700.0 μl of water (100 v/v) |
A4 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 200.0 μl of Sodium chloride (5 M) 700.0 μl of water (100 v/v) |
A5 |
| pH 8 | 200.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v) |
A6 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Sodium chloride (5 M) 700.0 μl of water (100 v/v) |
A7 |
| pH 4 | 400.0 μl of Sodium chloride (5 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
A8 |
| pH 5 | 400.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
A9 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v) |
A10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v) |
A11 |
| pH 8 | 400.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
A12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v) |
B1 |
| pH 4 | 600.0 μl of Sodium chloride (5 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
B2 |
| pH 5 | 600.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
B3 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
B4 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
B5 |
| pH 8 | 600.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
B6 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
B7 |
| pH 4 | 800.0 μl of Sodium chloride (5 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
B8 |
| pH 5 | 800.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
B9 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 800.0 μl of Sodium chloride (5 M) 100.0 μl of water (100 v/v) |
B10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 800.0 μl of Sodium chloride (5 M) 100.0 μl of water (100 v/v) |
B11 |
| pH 8 | 800.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 100.0 μl of water (100 v/v) |
B12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of Sodium chloride (5 M) 100.0 μl of water (100 v/v) |
C1 |
| pH 4 | 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 800.0 μl of water (100 v/v) |
C2 |
| pH 5 | 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 800.0 μl of water (100 v/v) |
C3 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 800.0 μl of water (100 v/v) |
C4 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 800.0 μl of water (100 v/v) |
C5 |
| pH 8 | 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 800.0 μl of water (100 v/v) |
C6 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 800.0 μl of water (100 v/v) |
C7 |
| pH 4 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
C8 |
| pH 5 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
C9 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
C10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
C11 |
| pH 8 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v) |
C12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
D1 |
| pH 4 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
D2 |
| pH 5 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
D3 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
D4 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
D5 |
| pH 8 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
D6 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
D7 |
| pH 4 | 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
D8 |
| pH 5 | 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
D9 |
| pH 6 | 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
D10 |
| pH 7 | 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
D11 |
| pH 8 | 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
D12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
E1 |
| pH 4 | 200.0 μl of Ammonium sulfate (4 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
E2 |
| pH 5 | 200.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
E3 |
| pH 6 | 200.0 μl of Ammonium sulfate (4 M) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 700.0 μl of water (100 v/v) |
E4 |
| pH 7 | 200.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 700.0 μl of water (100 v/v) |
E5 |
| pH 8 | 200.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v) |
E6 |
| pH 9 | 200.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 700.0 μl of water (100 v/v) |
E7 |
| pH 4 | 400.0 μl of Ammonium sulfate (4 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
E8 |
| pH 5 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
E9 |
| pH 6 | 400.0 μl of Ammonium sulfate (4 M) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 500.0 μl of water (100 v/v) |
E10 |
| pH 7 | 400.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 500.0 μl of water (100 v/v) |
E11 |
| pH 8 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
E12 |
| pH 9 | 400.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 500.0 μl of water (100 v/v) |
F1 |
| pH 4 | 600.0 μl of Ammonium sulfate (4 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
F2 |
| pH 5 | 600.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
F3 |
| pH 6 | 600.0 μl of Ammonium sulfate (4 M) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 300.0 μl of water (100 v/v) |
F4 |
| pH 7 | 600.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 300.0 μl of water (100 v/v) |
F5 |
| pH 8 | 600.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
F6 |
| pH 9 | 600.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 300.0 μl of water (100 v/v) |
F7 |
| pH 4 | 800.0 μl of Ammonium sulfate (4 M) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
F8 |
| pH 5 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
F9 |
| pH 6 | 800.0 μl of Ammonium sulfate (4 M) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 100.0 μl of water (100 v/v) |
F10 |
| pH 7 | 800.0 μl of Ammonium sulfate (4 M) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 100.0 μl of water (100 v/v) |
F11 |
| pH 8 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 100.0 μl of water (100 v/v) |
F12 |
| pH 9 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 100.0 μl of water (100 v/v) |
G1 |
| pH 4 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 800.0 μl of water (100 v/v) |
G2 |
| pH 5 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 800.0 μl of water (100 v/v) |
G3 |
| pH 6 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 800.0 μl of water (100 v/v) |
G4 |
| pH 7 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 800.0 μl of water (100 v/v) |
G5 |
| pH 8 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 800.0 μl of water (100 v/v) |
G6 |
| pH 9 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of water (100 v/v) |
G7 |
| pH 4 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
G8 |
| pH 5 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 700.0 μl of water (100 v/v) |
G9 |
| pH 6 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 700.0 μl of water (100 v/v) |
G10 |
| pH 7 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 700.0 μl of water (100 v/v) |
G11 |
| pH 8 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v) |
G12 |
| pH 9 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 700.0 μl of water (100 v/v) |
H1 |
| pH 4 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
H2 |
| pH 5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 500.0 μl of water (100 v/v) |
H3 |
| pH 6 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 500.0 μl of water (100 v/v) |
H4 |
| pH 7 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 500.0 μl of water (100 v/v) |
H5 |
| pH 8 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
H6 |
| pH 9 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 500.0 μl of water (100 v/v) |
H7 |
| pH 4 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 63.0 μl of tri-Sodium citrate pH 3.5 (1 M) 37.0 μl of tri-Sodium citrate pH 5 (1 M) 250.0 μl of water (100 v/v) |
H8 |
| pH 5 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 250.0 μl of water (100 v/v) |
H9 |
| pH 6 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57.5 μl of MES pH 6.5 (1 M) 42.5 μl of MES pH 5 (1 M) 250.0 μl of water (100 v/v) |
H10 |
| pH 7 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 77.7 μl of HEPES pH 6.5 (1 M) 22.3 μl of HEPES pH 8 (1 M) 250.0 μl of water (100 v/v) |
H11 |
| pH 8 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 250.0 μl of water (100 v/v) |
H12 |
| pH 9 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 250.0 μl of water (100 v/v) |