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Supplemental Information

Screen Description

pH Clear Suite II

Qiagen / pH Clear Suite II
Created 2009-11-16.

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pH Clear Suite II

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pH Clear Suite II

    Screen Structure

    pH Clear Suite II

    Well Count 96
    Number of distinct ingredients 10
    Internal diversity (score mean dist) 0.832

    Screen Composition

    More information about screen composition chart.
    2025-06-14T12:57:24.798193 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type pH Clear Suite II
    Buffer 96
    Organic (volatile) 24
    Polymer 18
    Salt 88

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    pH Clear Suite II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    		pH 4
    100 μl of Lithium chloride (10 M)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    A2
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    100 μl of Lithium chloride (10 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    A3
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    		pH 6
    100 μl of Lithium chloride (10 M)
    57 μl of MES pH 6.5 (1 M)
    42 μl of MES pH 5 (1 M)
    800 μl of water (100 v/v)
    A4
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    		pH 7
    100 μl of Lithium chloride (10 M)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    A5
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Lithium chloride (10 M)
    800 μl of water (100 v/v)
    A6
    • Lithium chloride 1 M -
    • BICINE 0.1 M pH 9
    		pH 9
    100 μl of Lithium chloride (10 M)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    A7
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 10 w/v -
    		pH 4
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    A8
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    A9
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 10 w/v -
    		pH 6
    100 μl of Lithium chloride (10 M)
    57 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    42 μl of MES pH 5 (1 M)
    600 μl of water (100 v/v)
    A10
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 10 w/v -
    		pH 7
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    A11
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 10 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    A12
    • Lithium chloride 1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 10 w/v -
    		pH 9
    100 μl of Lithium chloride (10 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    600 μl of water (100 v/v)
    B1
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of water (100 v/v)
    B2
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of water (100 v/v)
    B3
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 20 w/v -
    		pH 6
    100 μl of Lithium chloride (10 M)
    57 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    42 μl of MES pH 5 (1 M)
    400 μl of water (100 v/v)
    B4
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    		pH 7
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B5
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 20 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • Lithium chloride 1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 20 w/v -
    		pH 9
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    400 μl of water (100 v/v)
    B7
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 30 w/v -
    		pH 4
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of water (100 v/v)
    B8
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 5
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of water (100 v/v)
    B9
    • Lithium chloride 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 30 w/v -
    		pH 6
    100 μl of Lithium chloride (10 M)
    57 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    42 μl of MES pH 5 (1 M)
    200 μl of water (100 v/v)
    B10
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 30 w/v -
    		pH 7
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    22 μl of HEPES pH 8 (1 M)
    77 μl of HEPES pH 6.5 (1 M)
    200 μl of water (100 v/v)
    B11
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 30 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of water (100 v/v)
    B12
    • Lithium chloride 1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 30 w/v -
    		pH 9
    100 μl of Lithium chloride (10 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    200 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 4
    • 2-Propanol 5 v/v -
    		pH 4
    50 μl of 2-Propanol (100 v/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    850 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5
    • 2-Propanol 5 v/v -
    		pH 5
    50 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    850 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 6
    • 2-Propanol 5 v/v -
    		pH 6
    57 μl of MES pH 6.5 (1 M)
    42 μl of MES pH 5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    850 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7
    • 2-Propanol 5 v/v -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    50 μl of 2-Propanol (100 v/v)
    77 μl of HEPES pH 6.5 (1 M)
    850 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8
    • 2-Propanol 5 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of 2-Propanol (100 v/v)
    850 μl of water (100 v/v)
    C6
    • BICINE 0.1 M pH 9
    • 2-Propanol 5 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    50 μl of 2-Propanol (100 v/v)
    850 μl of water (100 v/v)
    C7
    • tri-Sodium citrate 0.1 M pH 4
    • 2-Propanol 10 v/v -
    		pH 4
    100 μl of 2-Propanol (100 v/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    C8
    • tri-Sodium citrate 0.1 M pH 5
    • 2-Propanol 10 v/v -
    		pH 5
    100 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    800 μl of water (100 v/v)
    C9
    • MES 0.1 M pH 6
    • 2-Propanol 10 v/v -
    		pH 6
    57 μl of MES pH 6.5 (1 M)
    42 μl of MES pH 5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    800 μl of water (100 v/v)
    C10
    • HEPES 0.1 M pH 7
    • 2-Propanol 10 v/v -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    100 μl of 2-Propanol (100 v/v)
    77 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    C11
    • tris chloride BCC 0.1 M pH 8
    • 2-Propanol 10 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of 2-Propanol (100 v/v)
    800 μl of water (100 v/v)
    C12
    • BICINE 0.1 M pH 9
    • 2-Propanol 10 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    100 μl of 2-Propanol (100 v/v)
    800 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 4
    • 2-Propanol 20 v/v -
    		pH 4
    200 μl of 2-Propanol (100 v/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M pH 5
    • 2-Propanol 20 v/v -
    		pH 5
    200 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6
    • 2-Propanol 20 v/v -
    		pH 6
    57 μl of MES pH 6.5 (1 M)
    42 μl of MES pH 5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    700 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7
    • 2-Propanol 20 v/v -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    200 μl of 2-Propanol (100 v/v)
    77 μl of HEPES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8
    • 2-Propanol 20 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of 2-Propanol (100 v/v)
    700 μl of water (100 v/v)
    D6
    • BICINE 0.1 M pH 9
    • 2-Propanol 20 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    200 μl of 2-Propanol (100 v/v)
    700 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 0.1 M pH 4
    • 2-Propanol 30 v/v -
    		pH 4
    300 μl of 2-Propanol (100 v/v)
    17 μl of tri-Sodium citrate pH 3.5 (1 M)
    83 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    D8
    • tri-Sodium citrate 0.1 M pH 5
    • 2-Propanol 30 v/v -
    		pH 5
    300 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of water (100 v/v)
    D9
    • MES 0.1 M pH 6
    • 2-Propanol 30 v/v -
    		pH 6
    57 μl of MES pH 6.5 (1 M)
    42 μl of MES pH 5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    D10
    • HEPES 0.1 M pH 7
    • 2-Propanol 30 v/v -
    		pH 7
    22 μl of HEPES pH 8 (1 M)
    300 μl of 2-Propanol (100 v/v)
    77 μl of HEPES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8
    • 2-Propanol 30 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    D12
    • BICINE 0.1 M pH 9
    • 2-Propanol 30 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    300 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    E1
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E2
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E3
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E4
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E5
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E6
    • Sodium Potassium phosphate 0.8 M -
    		NA
    800 μl of Sodium Potassium phosphate (1 M)
    200 μl of water (100 v/v)
    E7
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    E8
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    E9
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    E10
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    E11
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    E12
    • Sodium Potassium phosphate 1 M -
    		NA
    1000 μl of Sodium Potassium phosphate (1 M)
    F1
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F2
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F3
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F4
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F5
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F6
    • Sodium Potassium phosphate 1.4 M -
    		NA
    350 μl of Sodium Potassium phosphate (4 M)
    650 μl of water (100 v/v)
    F7
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    F8
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    F9
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    F10
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    F11
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    F12
    • Sodium Potassium phosphate 1.8 M -
    		NA
    450 μl of Sodium Potassium phosphate (4 M)
    550 μl of water (100 v/v)
    G1
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 7 (3.4 M)
    705 μl of water (100 v/v)
    G2
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 7 (3.4 M)
    558 μl of water (100 v/v)
    G3
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    G4
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    G5
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 7 (3.4 M)
    147 μl of water (100 v/v)
    G6
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 7 (3.4 M)
    G7
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 7 (3.4 M)
    705 μl of water (100 v/v)
    G8
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 7 (3.4 M)
    558 μl of water (100 v/v)
    G9
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    G10
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    G11
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 7 (3.4 M)
    147 μl of water (100 v/v)
    G12
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 7 (3.4 M)
    H1
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 7 (3.4 M)
    705 μl of water (100 v/v)
    H2
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 7 (3.4 M)
    558 μl of water (100 v/v)
    H3
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    H4
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    H5
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 7 (3.4 M)
    147 μl of water (100 v/v)
    H6
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 7 (3.4 M)
    H7
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 7 (3.4 M)
    705 μl of water (100 v/v)
    H8
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 7 (3.4 M)
    558 μl of water (100 v/v)
    H9
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    H10
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    H11
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 7 (3.4 M)
    147 μl of water (100 v/v)
    H12
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 7 (3.4 M)