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Supplemental Information

Screen Description

PreScreen

Qiagen / PreScreen
Created 2009-11-16.

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PreScreen

    Screen Structure

    PreScreen

    Well Count 24
    Number of distinct ingredients 11
    Internal diversity (score mean dist) 0.937

    Screen Composition

    2025-06-15T02:22:14.346624 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PreScreen
    Buffer 8
    Cryoprotectant 2
    Organic (non-volatile) 8
    Organic (volatile) 2
    Polymer 8
    Salt 13

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PreScreen Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 4.3 M -
    NA
    860 μl of Sodium chloride (5 M)
    140 μl of water (100 v/v)
    A2
    • Sodium chloride 1.5 M -
    NA
    300 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    A3
    • Ammonium sulfate 2 M -
    • Glycerol 30 v/v -
    NA
    300 μl of Glycerol (100 v/v)
    500 μl of Ammonium sulfate (4 M)
    200 μl of water (100 v/v)
    A4
    • Ammonium sulfate 2 M -
    • Glycerol 15 v/v -
    NA
    150 μl of Glycerol (100 v/v)
    500 μl of Ammonium sulfate (4 M)
    350 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 30 v/v -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    700 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 12 v/v -
    NA
    120 μl of Polyethylene glycol 400 (100 v/v)
    880 μl of water (100 v/v)
    B1
    • Ammonium sulfate 3.2 M -
    NA
    800 μl of Ammonium sulfate (4 M)
    200 μl of water (100 v/v)
    B2
    • Ammonium sulfate 1.1 M -
    NA
    275 μl of Ammonium sulfate (4 M)
    725 μl of water (100 v/v)
    B3
    • 2-Propanol 40 v/v -
    NA
    400 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    B4
    • 2-Propanol 15 v/v -
    NA
    150 μl of 2-Propanol (100 v/v)
    850 μl of water (100 v/v)
    B5
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • Polyethylene glycol 4,000 12 w/v -
    NA
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    760 μl of water (100 v/v)
    C1
    • Sodium Potassium phosphate 3 M -
    NA
    750 μl of Sodium Potassium phosphate (4 M)
    250 μl of water (100 v/v)
    C2
    • Sodium Potassium phosphate 1 M -
    NA
    1000 μl of Sodium Potassium phosphate (1 M)
    C3
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    NA
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    350 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 23 v/v -
    NA
    230 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    770 μl of water (100 v/v)
    C5
    • Polyethylene glycol 8,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Polyethylene glycol 8,000 12 w/v -
    NA
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    760 μl of water (100 v/v)
    D1
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    D2
    • Ammonium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    D3
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    D4
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 4,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    500 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    500 μl of water (100 v/v)