C6 - Screen Contents

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Screen Description

Basic 1

Basics / Basic 1

Created 2009-11-16.

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Basic 1

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Basic 1

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Screen Structure

Basic 1

Well Count	24
Number of distinct ingredients	27
Internal diversity (score mean dist)	0.935

Screen Composition

More information about screen composition chart.

ingredient type	Basic 1
Buffer	25
Cryoprotectant	1
Organic (non-volatile)	17
Polymer	13
Salt	27

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Basic 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ethylene glycol 25 v/v - 🔍	NA	250.0 μl of Ethylene glycol (100 v/v) 750.0 μl of water (100 v/v)
A2	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 1.5 M - Glycerol 12 v/v - 🔍	pH 8.5	500.0 μl of Ammonium sulfate (3 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 280.0 μl of water (100 v/v)
A3	Cobalt (II) chloride 0.01 M - Sodium acetate 0.1 M pH 4.6 1,6-Hexanediol 1 M - 🔍	pH 4.6	200.0 μl of 1,6-Hexanediol (5 M) 10.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 690.0 μl of water (100 v/v)
A4	tri-Sodium citrate 0.1 M pH 5.6 1,6-Hexanediol 2.5 M - 🔍	pH 5.6	500.0 μl of 1,6-Hexanediol (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v)
A5	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 1,6-Hexanediol 3.4 M - 🔍	pH 8.5	680.0 μl of 1,6-Hexanediol (5 M) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 140.0 μl of water (100 v/v)
A6	Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)
B1	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 5.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Ammonium acetate (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 560.0 μl of water (100 v/v)
B2	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20.0 μl of Calcium chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 580.0 μl of water (100 v/v)
B3	HEPES 0.1 M pH 7.5 Ammonium sulfate 0.5 M - 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 166.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 433.3 μl of water (100 v/v)
B4	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 500.0 μl of water (100 v/v)
B5	Ammonium di-hydrogen phosphate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium di-hydrogen phosphate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v)
B6	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 70 v/v - 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v)
C1	Sodium chloride 0.5 M - tri-Sodium citrate 0.1 M pH 5.6 Ethylene imine polymer 2 w/v - 🔍	pH 5.6	40.0 μl of Ethylene imine polymer (50 w/v) 500.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 360.0 μl of water (100 v/v)
C2	HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - Polyethylene glycol 400 2 v/v - 🔍	pH 7.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 213.3 μl of water (100 v/v)
C3	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 28 v/v - 🔍	pH 7.5	200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 420.0 μl of water (100 v/v)
C4	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 30 v/v - 🔍	pH 8.5	300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
C5	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 30 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v)
C6	Calcium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 30 v/v - 🔍	pH 4.6	100.0 μl of Calcium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v)
D1	Sodium chloride 0.1 M - BICINE 0.1 M pH 9.5 Polyethylene glycol monomethyl ether 550 20 v/v - 🔍	pH 9.5	100.0 μl of BICINE pH 9.5 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium chloride (1 M) 600.0 μl of water (100 v/v)
D2	Zinc sulfate 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 25 v/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 10.0 μl of Zinc sulfate (1 M) 640.0 μl of water (100 v/v)
D3	Polyethylene glycol 8,000 10 w/v - Polyethylene glycol 1,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)
D4	Polyethylene glycol 1,500 30 w/v - 🔍	NA	600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
D5	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 2,000 20 w/v - 🔍	pH 8.5	10.0 μl of Nickel (II) chloride (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v)
D6	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	pH 4.6	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v)