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Basic 2

Basics / Basic 2
Created 2009-11-16.

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Basic 2

    Screen Structure

    Basic 2

    Well Count 24
    Number of distinct ingredients 22
    Internal diversity (score mean dist) 0.889

    Screen Composition

    2025-06-13T21:33:16.970315 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Basic 2
    Buffer 24
    Organic (non-volatile) 2
    Organic (volatile) 2
    Polymer 24
    Salt 23

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Basic 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    A3
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    A4
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    333 μl of water (100 v/v)
    A5
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    A6
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Ammonium acetate (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    260 μl of water (100 v/v)
    B1
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Ammonium acetate (5 M)
    260 μl of water (100 v/v)
    B2
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Sodium acetate (2 M)
    200 μl of water (100 v/v)
    B3
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of Lithium sulfate (3 M)
    233 μl of water (100 v/v)
    B4
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    220 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    B6
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    400 μl of water (100 v/v)
    C1
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Polyethylene glycol 6,000 10 w/v -
    pH 7.5
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    C2
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 2 w/v -
    NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of Lithium sulfate (3 M)
    626 μl of water (100 v/v)
    C3
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 8 w/v -
    pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 8 v/v -
    • Polyethylene glycol 8,000 10 w/v -
    pH 7.5
    80 μl of Ethylene glycol (100 v/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    C5
    • Lithium sulfate 0.5 M -
    • Polyethylene glycol 8,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    166 μl of Lithium sulfate (3 M)
    533 μl of water (100 v/v)
    C6
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Calcium acetate (2 M)
    440 μl of water (100 v/v)
    D1
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Zinc acetate (2 M)
    440 μl of water (100 v/v)
    D2
    • Potassium di-hydrogen phosphate 0.05 M -
    • Polyethylene glycol 8,000 20 w/v -
    NA
    25 μl of Potassium di-hydrogen phosphate (2 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    575 μl of water (100 v/v)
    D3
    • Magnesium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    400 μl of water (100 v/v)
    D4
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    80 μl of Sodium acetate (2.5 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    220 μl of water (100 v/v)
    D5
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    D6
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)