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Basic 2

Basics / Basic 2
Created 2009-11-16.

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Basic 2

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Screen Structure

Basic 2

Well Count 24
Number of distinct ingredients 22
Internal diversity (score mean dist) 0.889

Screen Composition

2025-08-16T09:05:38.562021 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Basic 2
Buffer 24
Organic (non-volatile) 2
Organic (volatile) 2
Polymer 24
Salt 23

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Basic 2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
A3
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
A4
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
333.3 μl of water (100 v/v)
A5
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
333.3 μl of water (100 v/v)
A6
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
40.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
260.0 μl of water (100 v/v)
B1
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
pH 5.6
40.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
260.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
B3
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B4
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
B5
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
233.3 μl of water (100 v/v)
B6
  • Sodium chloride 2 M -
  • Polyethylene glycol 6,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
C1
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • Polyethylene glycol 6,000 10 w/v -
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
C2
  • Lithium sulfate 1 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
333.3 μl of Lithium sulfate (3 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
626.7 μl of water (100 v/v)
C3
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 8 v/v -
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
C5
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
166.7 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
533.3 μl of water (100 v/v)
C6
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
440.0 μl of water (100 v/v)
D1
  • Zinc acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Zinc acetate (2 M)
440.0 μl of water (100 v/v)
D2
  • Potassium di-hydrogen phosphate 0.05 M -
  • Polyethylene glycol 8,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium di-hydrogen phosphate (2 M)
575.0 μl of water (100 v/v)
D3
  • Magnesium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
D4
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
220.0 μl of water (100 v/v)
D5
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
D6
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
233.3 μl of water (100 v/v)