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Supplemental Information

Screen Description

Basic 3

Basics / Basic 3
Created 2009-11-16.

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Basic 3

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Basic 3

    Screen Structure

    Basic 3

    Well Count 24
    Number of distinct ingredients 25
    Internal diversity (score mean dist) 0.952

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:23:02.960417 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Basic 3
    Buffer 23
    Organic (non-volatile) 5
    Organic (volatile) 10
    Polymer 6
    Salt 19

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Basic 3 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • BICINE 0.1 M pH 9.5
    • 1,4-Dioxane 2 v/v -
    • Polyethylene glycol 10,000 10 w/v -
    		pH 9.5
    40 μl of 1,4-Dioxane (50 v/v)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BICINE pH 9.5 (1 M)
    660 μl of water (100 v/v)
    A2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    A3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 20,000 (50 w/v)
    660 μl of water (100 v/v)
    A4
    • Ammonium sulfate 2 M -
    • 2-Propanol 5 v/v -
    		NA
    50 μl of 2-Propanol (100 v/v)
    666 μl of Ammonium sulfate (3 M)
    283 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 20 v/v -
    		pH 7.5
    100 μl of tri-Sodium citrate (2 M)
    200 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of water (100 v/v)
    A6
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 20 v/v -
    		pH 4.6
    200 μl of 2-Propanol (100 v/v)
    200 μl of Calcium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    B1
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 30 v/v -
    		pH 7.5
    300 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    520 μl of water (100 v/v)
    B2
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 30 v/v -
    		pH 8.5
    300 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Ammonium acetate (5 M)
    560 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.6 M -
    • 1,4-Dioxane 10 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of 1,4-Dioxane (50 v/v)
    533 μl of Ammonium sulfate (3 M)
    166 μl of water (100 v/v)
    B4
    • 1,4-Dioxane 35 v/v -
    		NA
    700 μl of 1,4-Dioxane (50 v/v)
    300 μl of water (100 v/v)
    B5
    • Sodium chloride 1.5 M -
    • Ethanol 10 v/v -
    		NA
    200 μl of Ethanol (50 v/v)
    300 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    		pH 8.5
    400 μl of Ethanol (50 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Methyl-2-propanol 25 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of 2-Methyl-2-propanol (50 v/v)
    400 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Methyl-2-propanol 35 v/v -
    		pH 5.6
    700 μl of 2-Methyl-2-propanol (50 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of water (100 v/v)
    C3
    • Imidazole 1 M -
    		NA
    1000 μl of Imidazole pH 7 (1 M)
    C4
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    		pH 8.5
    10 μl of Nickel (II) chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Lithium sulfate (3 M)
    556 μl of water (100 v/v)
    C5
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Lithium sulfate (3 M)
    400 μl of water (100 v/v)
    C6
    • Potassium Sodium tartrate 0.4 M -
    		NA
    200 μl of Potassium Sodium tartrate (2 M)
    800 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.8 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Potassium Sodium tartrate (2 M)
    500 μl of water (100 v/v)
    D2
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    		pH 7.5
    280 μl of tri-Sodium citrate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    D3
    • tri-Sodium citrate 1.6 M -
    		NA
    800 μl of tri-Sodium citrate pH 6.5 (2 M)
    200 μl of water (100 v/v)
    D4
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    		pH 5.6
    200 μl of Jeffamine® M-600 (50 v/v)
    10 μl of Iron (III) chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    690 μl of water (100 v/v)
    D5
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    		pH 7.5
    400 μl of Jeffamine® M-600 (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    D6
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    		pH 6.5
    600 μl of Jeffamine® M-600 (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    50 μl of Cesium chloride (1 M)
    250 μl of water (100 v/v)