C6 - Screen Contents

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Screen Description

Basic 3

Basics / Basic 3

Created 2009-11-16.

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Basic 3

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Basic 3

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Screen Structure

Basic 3

Well Count	24
Number of distinct ingredients	25
Internal diversity (score mean dist)	0.952

Screen Composition

More information about screen composition chart.

ingredient type	Basic 3
Buffer	23
Organic (non-volatile)	5
Organic (volatile)	10
Polymer	6
Salt	19

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Basic 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	BICINE 0.1 M pH 9.5 1,4-Dioxane 2 v/v - Polyethylene glycol 10,000 10 w/v - 🔍	pH 9.5	40.0 μl of 1,4-Dioxane (50 v/v) 100.0 μl of BICINE pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 660.0 μl of water (100 v/v)
A2	HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v)
A3	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
A4	Ammonium sulfate 2 M - 2-Propanol 5 v/v - 🔍	NA	50.0 μl of 2-Propanol (100 v/v) 666.7 μl of Ammonium sulfate (3 M) 283.3 μl of water (100 v/v)
A5	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 20 v/v - 🔍	pH 7.5	200.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 600.0 μl of water (100 v/v)
A6	Calcium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-Propanol 20 v/v - 🔍	pH 4.6	200.0 μl of 2-Propanol (100 v/v) 200.0 μl of Calcium chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v)
B1	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 30 v/v - 🔍	pH 7.5	300.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 520.0 μl of water (100 v/v)
B2	Ammonium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-Propanol 30 v/v - 🔍	pH 8.5	300.0 μl of 2-Propanol (100 v/v) 40.0 μl of Ammonium acetate (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 560.0 μl of water (100 v/v)
B3	MES 0.1 M pH 6.5 Ammonium sulfate 1.6 M - 1,4-Dioxane 10 v/v - 🔍	pH 6.5	200.0 μl of 1,4-Dioxane (50 v/v) 533.3 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 166.7 μl of water (100 v/v)
B4	1,4-Dioxane 35 v/v - 🔍	NA	700.0 μl of 1,4-Dioxane (50 v/v) 300.0 μl of water (100 v/v)
B5	Sodium chloride 1.5 M - Ethanol 10 v/v - 🔍	NA	200.0 μl of Ethanol (50 v/v) 300.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
B6	tris chloride BCC 0.1 M pH 8.5 Ethanol 20 v/v - 🔍	pH 8.5	400.0 μl of Ethanol (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
C1	tris chloride BCC 0.1 M pH 8.5 2-Methyl-2-propanol 25 v/v - 🔍	pH 8.5	500.0 μl of 2-Methyl-2-propanol (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C2	tri-Sodium citrate 0.1 M pH 5.6 2-Methyl-2-propanol 35 v/v - 🔍	pH 5.6	700.0 μl of 2-Methyl-2-propanol (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 200.0 μl of water (100 v/v)
C3	Imidazole 1 M - 🔍	NA	1000.0 μl of Imidazole pH 7 (1 M)
C4	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1 M - 🔍	pH 8.5	333.3 μl of Lithium sulfate (3 M) 10.0 μl of Nickel (II) chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 556.7 μl of water (100 v/v)
C5	HEPES 0.1 M pH 7.5 Lithium sulfate 1.5 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Lithium sulfate (3 M) 400.0 μl of water (100 v/v)
C6	Potassium Sodium tartrate 0.4 M - 🔍	NA	200.0 μl of Potassium Sodium tartrate (2 M) 800.0 μl of water (100 v/v)
D1	HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 0.8 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Potassium Sodium tartrate (2 M) 500.0 μl of water (100 v/v)
D2	HEPES 0.1 M pH 7.5 tri-Sodium citrate 1.4 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of tri-Sodium citrate (5 M) 620.0 μl of water (100 v/v)
D3	tri-Sodium citrate 1.6 M - 🔍	NA	800.0 μl of tri-Sodium citrate pH 6.5 (2 M) 200.0 μl of water (100 v/v)
D4	Iron (III) chloride 0.01 M - tri-Sodium citrate 0.1 M pH 5.6 Jeffamine® M-600 10 v/v - 🔍	pH 5.6	10.0 μl of Iron (III) chloride (1 M) 200.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 690.0 μl of water (100 v/v)
D5	HEPES 0.1 M pH 7.5 Jeffamine® M-600 20 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Jeffamine® M-600 (50 v/v) 500.0 μl of water (100 v/v)
D6	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)