C6 - Screen Contents

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Screen Description

Basic 4

Basics / Basic 4

Created 2009-11-16.

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Basic 4

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Basic 4

Screen Structure

Basic 4

Well Count	24
Number of distinct ingredients	22
Internal diversity (score mean dist)	0.936

Screen Composition

More information about screen composition chart.

ingredient type	Basic 4
Buffer	33
Detergent	1
Salt	39

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Basic 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	HEPES 0.1 M pH 7.5 Potassium di-hydrogen phosphate 0.8 M - Sodium di-hydrogen phosphate 0.8 M - 🔍	pH 7.5	200 μl of Sodium di-hydrogen phosphate (4 M) 400 μl of Potassium di-hydrogen phosphate (2 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v)
A2	Ammonium di-hydrogen phosphate 0.4 M - 🔍	NA	133 μl of Ammonium di-hydrogen phosphate (3 M) 866 μl of water (100 v/v)
A3	tri-Sodium citrate 0.1 M pH 5.6 Ammonium di-hydrogen phosphate 1 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 333 μl of Ammonium di-hydrogen phosphate (3 M) 566 μl of water (100 v/v)
A4	tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 2 M - 🔍	pH 8.5	666 μl of Ammonium di-hydrogen phosphate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)
A5	Sodium acetate 0.1 M pH 4.6 Ammonium formate 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of Ammonium formate (5 M) 500 μl of water (100 v/v)
A6	HEPES 0.1 M pH 7.5 Ammonium formate 4 M - 🔍	pH 7.5	800 μl of Ammonium formate (5 M) 100 μl of HEPES pH 7.5 (1 M) 100 μl of water (100 v/v)
B1	Ammonium formate 2 M - 🔍	NA	400 μl of Ammonium formate (5 M) 600 μl of water (100 v/v)
B2	tri-Sodium citrate 0.1 M pH 5.6 Lithium sulfate 1 M - Ammonium sulfate 0.5 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 166 μl of Ammonium sulfate (3 M) 333 μl of Lithium sulfate (3 M) 400 μl of water (100 v/v)
B3	Sodium chloride 0.1 M - HEPES 0.1 M pH 7.5 Ammonium sulfate 1.6 M - 🔍	pH 7.5	100 μl of Sodium chloride (1 M) 533 μl of Ammonium sulfate (3 M) 100 μl of HEPES pH 7.5 (1 M) 266 μl of water (100 v/v)
B4	Cobalt (II) chloride 0.01 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	600 μl of Ammonium sulfate (3 M) 10 μl of Cobalt (II) chloride (1 M) 100 μl of MES pH 6.5 (1 M) 290 μl of water (100 v/v)
B5	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 2 M - 🔍	pH 8.5	666 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)
B6	Ammonium sulfate 2 M - 🔍	NA	666 μl of Ammonium sulfate (3 M) 333 μl of water (100 v/v)
C1	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666 μl of Ammonium sulfate (3 M) 100 μl of Sodium acetate pH 4.6 (1 M) 233 μl of water (100 v/v)
C2	Potassium Sodium tartrate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 2 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 666 μl of Ammonium sulfate (3 M) 100 μl of Potassium Sodium tartrate (2 M) 133 μl of water (100 v/v)
C3	Magnesium formate 0.2 M - 🔍	NA	200 μl of Magnesium formate (1 M) 800 μl of water (100 v/v)
C4	MES 0.1 M pH 6.5 Magnesium sulfate 1.6 M - 🔍	pH 6.5	800 μl of Magnesium sulfate (2 M) 100 μl of MES pH 6.5 (1 M) 100 μl of water (100 v/v)
C5	BICINE 0.1 M pH 9.5 Magnesium chloride 2 M - 🔍	pH 9.5	800 μl of Magnesium chloride (2.5 M) 100 μl of BICINE pH 9.5 (1 M) 100 μl of water (100 v/v)
C6	Imidazole 0.1 M pH 6.5 Sodium acetate 1 M - 🔍	pH 6.5	100 μl of Imidazole pH 6.5 (1 M) 400 μl of Sodium acetate (2.5 M) 500 μl of water (100 v/v)
D1	Cadmium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Sodium acetate 1 M - 🔍	pH 7.5	50 μl of Cadmium sulfate (1 M) 400 μl of Sodium acetate (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v)
D2	MES 0.1 M pH 6.5 Sodium acetate 1.4 M - 🔍	pH 6.5	560 μl of Sodium acetate (2.5 M) 100 μl of MES pH 6.5 (1 M) 340 μl of water (100 v/v)
D3	Cetyltrimethylammonium bromide 0.01 M - Magnesium chloride 0.01 M - Sodium chloride 0.5 M - 🔍	NA	4 μl of Magnesium chloride (2.5 M) 200 μl of Cetyltrimethylammonium bromide (0.05 M) 100 μl of Sodium chloride (5 M) 696 μl of water (100 v/v)
D4	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of Sodium chloride (5 M) 500 μl of water (100 v/v)
D5	Sodium di-hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 6.5	25 μl of Sodium di-hydrogen phosphate (4 M) 50 μl of Potassium di-hydrogen phosphate (2 M) 400 μl of Sodium chloride (5 M) 100 μl of MES pH 6.5 (1 M) 425 μl of water (100 v/v)
D6	HEPES 0.1 M pH 7.5 Sodium chloride 4.3 M - 🔍	pH 7.5	860 μl of Sodium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 40 μl of water (100 v/v)