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Supplemental Information

Screen Description

Basic 4

Basics / Basic 4
Created 2009-11-16.

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Basic 4

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Basic 4

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Screen Structure

Basic 4

Well Count 24
Number of distinct ingredients 22
Internal diversity (score mean dist) 0.936

Screen Composition

More information about screen composition chart.
2025-08-05T17:38:50.323457 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Basic 4
Buffer 33
Detergent 1
Salt 39

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Basic 4 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • HEPES 0.1 M pH 7.5
  • Potassium di-hydrogen phosphate 0.8 M -
  • Sodium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium di-hydrogen phosphate (2 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
300.0 μl of water (100 v/v)
A2
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
133.3 μl of Ammonium di-hydrogen phosphate (3 M)
866.7 μl of water (100 v/v)
A3
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
566.7 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
666.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium formate 2 M -
pH 4.6
400.0 μl of Ammonium formate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
A6
  • HEPES 0.1 M pH 7.5
  • Ammonium formate 4 M -
pH 7.5
800.0 μl of Ammonium formate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of water (100 v/v)
B1
  • Ammonium formate 2 M -
NA
400.0 μl of Ammonium formate (5 M)
600.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 1 M -
  • Ammonium sulfate 0.5 M -
pH 5.6
166.7 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
B3
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Sodium chloride (1 M)
266.7 μl of water (100 v/v)
B4
  • Cobalt (II) chloride 0.01 M -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
600.0 μl of Ammonium sulfate (3 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
290.0 μl of water (100 v/v)
B5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B6
  • Ammonium sulfate 2 M -
NA
666.7 μl of Ammonium sulfate (3 M)
333.3 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C2
  • Potassium Sodium tartrate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
133.3 μl of water (100 v/v)
C3
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
800.0 μl of water (100 v/v)
C4
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
C5
  • BICINE 0.1 M pH 9.5
  • Magnesium chloride 2 M -
pH 9.5
100.0 μl of BICINE pH 9.5 (1 M)
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of water (100 v/v)
C6
  • Imidazole 0.1 M pH 6.5
  • Sodium acetate 1 M -
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
400.0 μl of Sodium acetate (2.5 M)
500.0 μl of water (100 v/v)
D1
  • Cadmium sulfate 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1 M -
pH 7.5
50.0 μl of Cadmium sulfate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Sodium acetate (2.5 M)
450.0 μl of water (100 v/v)
D2
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
560.0 μl of Sodium acetate (2.5 M)
340.0 μl of water (100 v/v)
D3
  • Cetyltrimethylammonium bromide 0.01 M -
  • Magnesium chloride 0.01 M -
  • Sodium chloride 0.5 M -
NA
200.0 μl of Cetyltrimethylammonium bromide (0.05 M)
4.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium chloride (5 M)
696.0 μl of water (100 v/v)
D4
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
D5
  • Sodium di-hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
  • Potassium di-hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
400.0 μl of Sodium chloride (5 M)
25.0 μl of Sodium di-hydrogen phosphate (4 M)
425.0 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)