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Screen Description

Basic HTS

Basics / Basic HTS
Created 2009-11-16.

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Basic HTS

    Screen Structure

    Basic HTS

    Well Count 96
    Number of distinct ingredients 53
    Internal diversity (score mean dist) 0.950

    Screen Composition

    2025-06-14T13:14:53.435705 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Basic HTS
    Buffer 105
    Cryoprotectant 1
    Detergent 1
    Organic (non-volatile) 24
    Organic (volatile) 12
    Polymer 43
    Salt 108

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Basic HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ethylene glycol 25 v/v -
    NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    A2
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.5 M -
    • Glycerol 12 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    120 μl of Glycerol (100 v/v)
    500 μl of Ammonium sulfate (3 M)
    280 μl of water (100 v/v)
    A3
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.1 M pH 4.6
    • 1,6-Hexanediol 1 M -
    pH 4.6
    10 μl of Cobalt (II) chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of 1,6-Hexanediol (5 M)
    690 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.5 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of 1,6-Hexanediol (5 M)
    400 μl of water (100 v/v)
    A5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    680 μl of 1,6-Hexanediol (5 M)
    140 μl of water (100 v/v)
    A6
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Sodium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    A7
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 5.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Ammonium acetate (5 M)
    560 μl of water (100 v/v)
    A8
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    20 μl of Calcium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    580 μl of water (100 v/v)
    A9
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.5 M -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    166 μl of Ammonium sulfate (3 M)
    433 μl of water (100 v/v)
    A10
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    A11
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    200 μl of water (100 v/v)
    A12
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    B1
    • Sodium chloride 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ethylene imine polymer 2 w/v -
    pH 5.6
    500 μl of Sodium chloride (1 M)
    40 μl of Ethylene imine polymer (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    360 μl of water (100 v/v)
    B2
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 2 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    666 μl of Ammonium sulfate (3 M)
    213 μl of water (100 v/v)
    B3
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Calcium chloride (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    420 μl of water (100 v/v)
    B4
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of tri-Sodium citrate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    B6
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    pH 4.6
    100 μl of Calcium chloride (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    B7
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9.5
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 9.5
    100 μl of BICINE pH 9.5 (1 M)
    100 μl of Sodium chloride (1 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    600 μl of water (100 v/v)
    B8
    • Zinc sulfate 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    pH 6.5
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    10 μl of Zinc sulfate (1 M)
    100 μl of MES pH 6.5 (1 M)
    640 μl of water (100 v/v)
    B9
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    B10
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B11
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    10 μl of Nickel (II) chloride (1 M)
    490 μl of water (100 v/v)
    B12
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 4.6
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    C1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    C3
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    C4
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    C5
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    C6
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Ammonium acetate (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    260 μl of water (100 v/v)
    C7
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    40 μl of Ammonium acetate (5 M)
    260 μl of water (100 v/v)
    C8
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate (2 M)
    200 μl of water (100 v/v)
    C9
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    233 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    220 μl of water (100 v/v)
    C11
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    233 μl of water (100 v/v)
    C12
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    400 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Polyethylene glycol 6,000 10 w/v -
    pH 7.5
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    D2
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 2 w/v -
    NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of Lithium sulfate (3 M)
    626 μl of water (100 v/v)
    D3
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 8 w/v -
    pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 8 v/v -
    • Polyethylene glycol 8,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    D5
    • Lithium sulfate 0.5 M -
    • Polyethylene glycol 8,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    166 μl of Lithium sulfate (3 M)
    533 μl of water (100 v/v)
    D6
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Calcium acetate (2 M)
    100 μl of MES pH 6.5 (1 M)
    440 μl of water (100 v/v)
    D7
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Zinc acetate (2 M)
    100 μl of MES pH 6.5 (1 M)
    440 μl of water (100 v/v)
    D8
    • Potassium di-hydrogen phosphate 0.05 M -
    • Polyethylene glycol 8,000 20 w/v -
    NA
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Potassium di-hydrogen phosphate (2 M)
    575 μl of water (100 v/v)
    D9
    • Magnesium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    100 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    D10
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Sodium acetate (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    220 μl of water (100 v/v)
    D11
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    D12
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    233 μl of water (100 v/v)
    E1
    • BICINE 0.1 M pH 9.5
    • 1,4-Dioxane 2 v/v -
    • Polyethylene glycol 10,000 10 w/v -
    pH 9.5
    100 μl of BICINE pH 9.5 (1 M)
    40 μl of 1,4-Dioxane (50 v/v)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    660 μl of water (100 v/v)
    E2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    E3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    660 μl of water (100 v/v)
    E4
    • Ammonium sulfate 2 M -
    • 2-Propanol 5 v/v -
    NA
    50 μl of 2-Propanol (100 v/v)
    666 μl of Ammonium sulfate (3 M)
    283 μl of water (100 v/v)
    E5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 20 v/v -
    pH 7.5
    100 μl of tri-Sodium citrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    E6
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-Propanol 20 v/v -
    pH 4.6
    200 μl of Calcium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    500 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 30 v/v -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    520 μl of water (100 v/v)
    E8
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 30 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Ammonium acetate (5 M)
    300 μl of 2-Propanol (100 v/v)
    560 μl of water (100 v/v)
    E9
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.6 M -
    • 1,4-Dioxane 10 v/v -
    pH 6.5
    200 μl of 1,4-Dioxane (50 v/v)
    533 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    166 μl of water (100 v/v)
    E10
    • 1,4-Dioxane 35 v/v -
    NA
    700 μl of 1,4-Dioxane (50 v/v)
    300 μl of water (100 v/v)
    E11
    • Sodium chloride 1.5 M -
    • Ethanol 10 v/v -
    NA
    200 μl of Ethanol (50 v/v)
    300 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    E12
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 20 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ethanol (50 v/v)
    500 μl of water (100 v/v)
    F1
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Methyl-2-propanol 25 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of 2-Methyl-2-propanol (50 v/v)
    400 μl of water (100 v/v)
    F2
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Methyl-2-propanol 35 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    700 μl of 2-Methyl-2-propanol (50 v/v)
    200 μl of water (100 v/v)
    F3
    • Imidazole 1 M -
    NA
    1000 μl of Imidazole pH 7 (1 M)
    F4
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Lithium sulfate (3 M)
    10 μl of Nickel (II) chloride (1 M)
    556 μl of water (100 v/v)
    F5
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    pH 7.5
    500 μl of Lithium sulfate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    F6
    • Potassium Sodium tartrate 0.4 M -
    NA
    200 μl of Potassium Sodium tartrate (2 M)
    800 μl of water (100 v/v)
    F7
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.8 M -
    pH 7.5
    400 μl of Potassium Sodium tartrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    F8
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    pH 7.5
    280 μl of tri-Sodium citrate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    F9
    • tri-Sodium citrate 1.6 M -
    NA
    800 μl of tri-Sodium citrate pH 6.5 (2 M)
    200 μl of water (100 v/v)
    F10
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Jeffamine® M-600 10 v/v -
    pH 5.6
    200 μl of Jeffamine® M-600 (50 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    10 μl of Iron (III) chloride (1 M)
    690 μl of water (100 v/v)
    F11
    • HEPES 0.1 M pH 7.5
    • Jeffamine® M-600 20 v/v -
    pH 7.5
    400 μl of Jeffamine® M-600 (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    F12
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    pH 6.5
    600 μl of Jeffamine® M-600 (50 v/v)
    50 μl of Cesium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    G1
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 0.8 M -
    • Sodium di-hydrogen phosphate 0.8 M -
    pH 7.5
    400 μl of Potassium di-hydrogen phosphate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Sodium di-hydrogen phosphate (4 M)
    300 μl of water (100 v/v)
    G2
    • Ammonium di-hydrogen phosphate 0.4 M -
    NA
    133 μl of Ammonium di-hydrogen phosphate (3 M)
    866 μl of water (100 v/v)
    G3
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 1 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    333 μl of Ammonium di-hydrogen phosphate (3 M)
    566 μl of water (100 v/v)
    G4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Ammonium di-hydrogen phosphate (3 M)
    233 μl of water (100 v/v)
    G5
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium formate 2 M -
    pH 4.6
    400 μl of Ammonium formate (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    G6
    • HEPES 0.1 M pH 7.5
    • Ammonium formate 4 M -
    pH 7.5
    800 μl of Ammonium formate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    G7
    • Ammonium formate 2 M -
    NA
    400 μl of Ammonium formate (5 M)
    600 μl of water (100 v/v)
    G8
    • tri-Sodium citrate 0.1 M pH 5.6
    • Lithium sulfate 1 M -
    • Ammonium sulfate 0.5 M -
    pH 5.6
    333 μl of Lithium sulfate (3 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    166 μl of Ammonium sulfate (3 M)
    400 μl of water (100 v/v)
    G9
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    pH 7.5
    100 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Ammonium sulfate (3 M)
    266 μl of water (100 v/v)
    G10
    • Cobalt (II) chloride 0.01 M -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    pH 6.5
    10 μl of Cobalt (II) chloride (1 M)
    600 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    290 μl of water (100 v/v)
    G11
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    G12
    • Ammonium sulfate 2 M -
    NA
    666 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    H1
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    H2
    • Potassium Sodium tartrate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    pH 5.6
    100 μl of Potassium Sodium tartrate (2 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    133 μl of water (100 v/v)
    H3
    • Magnesium formate 0.2 M -
    NA
    200 μl of Magnesium formate (1 M)
    800 μl of water (100 v/v)
    H4
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    pH 6.5
    800 μl of Magnesium sulfate (2 M)
    100 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    H5
    • BICINE 0.1 M pH 9.5
    • Magnesium chloride 2 M -
    pH 9.5
    100 μl of BICINE pH 9.5 (1 M)
    800 μl of Magnesium chloride (2.5 M)
    100 μl of water (100 v/v)
    H6
    • Imidazole 0.1 M pH 6.5
    • Sodium acetate 1 M -
    pH 6.5
    400 μl of Sodium acetate (2.5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    500 μl of water (100 v/v)
    H7
    • Cadmium sulfate 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Sodium acetate (2.5 M)
    50 μl of Cadmium sulfate (1 M)
    450 μl of water (100 v/v)
    H8
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    560 μl of Sodium acetate (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    340 μl of water (100 v/v)
    H9
    • Cetyltrimethylammonium bromide 0.01 M -
    • Magnesium chloride 0.01 M -
    • Sodium chloride 0.5 M -
    NA
    4 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium chloride (5 M)
    200 μl of Cetyltrimethylammonium bromide (0.05 M)
    696 μl of water (100 v/v)
    H10
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    pH 4.6
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    H11
    • Sodium di-hydrogen phosphate 0.1 M -
    • MES 0.1 M pH 6.5
    • Sodium chloride 2 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    pH 6.5
    50 μl of Potassium di-hydrogen phosphate (2 M)
    25 μl of Sodium di-hydrogen phosphate (4 M)
    400 μl of Sodium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    425 μl of water (100 v/v)
    H12
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 4.3 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    860 μl of Sodium chloride (5 M)
    40 μl of water (100 v/v)