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Supplemental Information

Screen Description

Basic HTS

Basics / Basic HTS
Created 2009-11-16.

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Basic HTS

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Screen Structure

Basic HTS

Well Count 96
Number of distinct ingredients 53
Internal diversity (score mean dist) 0.950

Screen Composition

2025-08-16T04:27:15.560818 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Basic HTS
Buffer 105
Cryoprotectant 1
Detergent 1
Organic (non-volatile) 24
Organic (volatile) 12
Polymer 43
Salt 108

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Basic HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
A2
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.5 M -
  • Glycerol 12 v/v -
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
120.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
280.0 μl of water (100 v/v)
A3
  • Cobalt (II) chloride 0.01 M -
  • Sodium acetate 0.1 M pH 4.6
  • 1,6-Hexanediol 1 M -
pH 4.6
200.0 μl of 1,6-Hexanediol (5 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
690.0 μl of water (100 v/v)
A4
  • tri-Sodium citrate 0.1 M pH 5.6
  • 1,6-Hexanediol 2.5 M -
pH 5.6
500.0 μl of 1,6-Hexanediol (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
A5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 1,6-Hexanediol 3.4 M -
pH 8.5
680.0 μl of 1,6-Hexanediol (5 M)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
140.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
200.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
A7
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Ammonium acetate (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
560.0 μl of water (100 v/v)
A8
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of Calcium chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
580.0 μl of water (100 v/v)
A9
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.5 M -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
166.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
433.3 μl of water (100 v/v)
A10
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of tri-Sodium citrate (2 M)
500.0 μl of water (100 v/v)
A11
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A12
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
B1
  • Sodium chloride 0.5 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ethylene imine polymer 2 w/v -
pH 5.6
40.0 μl of Ethylene imine polymer (50 w/v)
500.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
360.0 μl of water (100 v/v)
B2
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 2 v/v -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
213.3 μl of water (100 v/v)
B3
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
pH 7.5
200.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
420.0 μl of water (100 v/v)
B4
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
B6
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
pH 4.6
100.0 μl of Calcium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
B7
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 9.5
100.0 μl of BICINE pH 9.5 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium chloride (1 M)
600.0 μl of water (100 v/v)
B8
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
10.0 μl of Zinc sulfate (1 M)
640.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
B11
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
10.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
B12
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
C3
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
C4
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
333.3 μl of water (100 v/v)
C5
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
333.3 μl of water (100 v/v)
C6
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
40.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
260.0 μl of water (100 v/v)
C7
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 30 w/v -
pH 5.6
40.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
260.0 μl of water (100 v/v)
C8
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C9
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
C10
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
C11
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
233.3 μl of water (100 v/v)
C12
  • Sodium chloride 2 M -
  • Polyethylene glycol 6,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • Polyethylene glycol 6,000 10 w/v -
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
650.0 μl of water (100 v/v)
D2
  • Lithium sulfate 1 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
333.3 μl of Lithium sulfate (3 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
626.7 μl of water (100 v/v)
D3
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 8 v/v -
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
D5
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
166.7 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
533.3 μl of water (100 v/v)
D6
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
440.0 μl of water (100 v/v)
D7
  • Zinc acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Zinc acetate (2 M)
440.0 μl of water (100 v/v)
D8
  • Potassium di-hydrogen phosphate 0.05 M -
  • Polyethylene glycol 8,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium di-hydrogen phosphate (2 M)
575.0 μl of water (100 v/v)
D9
  • Magnesium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
D10
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
220.0 μl of water (100 v/v)
D11
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
D12
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
233.3 μl of water (100 v/v)
E1
  • BICINE 0.1 M pH 9.5
  • 1,4-Dioxane 2 v/v -
  • Polyethylene glycol 10,000 10 w/v -
pH 9.5
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
660.0 μl of water (100 v/v)
E2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
E3
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 2 M -
  • 2-Propanol 5 v/v -
NA
50.0 μl of 2-Propanol (100 v/v)
666.7 μl of Ammonium sulfate (3 M)
283.3 μl of water (100 v/v)
E5
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 20 v/v -
pH 7.5
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of tri-Sodium citrate (2 M)
600.0 μl of water (100 v/v)
E6
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-Propanol 20 v/v -
pH 4.6
200.0 μl of 2-Propanol (100 v/v)
200.0 μl of Calcium chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
E7
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 30 v/v -
pH 7.5
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
520.0 μl of water (100 v/v)
E8
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 30 v/v -
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
40.0 μl of Ammonium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
E9
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.6 M -
  • 1,4-Dioxane 10 v/v -
pH 6.5
200.0 μl of 1,4-Dioxane (50 v/v)
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
166.7 μl of water (100 v/v)
E10
  • 1,4-Dioxane 35 v/v -
NA
700.0 μl of 1,4-Dioxane (50 v/v)
300.0 μl of water (100 v/v)
E11
  • Sodium chloride 1.5 M -
  • Ethanol 10 v/v -
NA
200.0 μl of Ethanol (50 v/v)
300.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
E12
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 20 v/v -
pH 8.5
400.0 μl of Ethanol (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
F1
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Methyl-2-propanol 25 v/v -
pH 8.5
500.0 μl of 2-Methyl-2-propanol (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
F2
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Methyl-2-propanol 35 v/v -
pH 5.6
700.0 μl of 2-Methyl-2-propanol (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
200.0 μl of water (100 v/v)
F3
  • Imidazole 1 M -
NA
1000.0 μl of Imidazole pH 7 (1 M)
F4
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1 M -
pH 8.5
333.3 μl of Lithium sulfate (3 M)
10.0 μl of Nickel (II) chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
556.7 μl of water (100 v/v)
F5
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Lithium sulfate (3 M)
400.0 μl of water (100 v/v)
F6
  • Potassium Sodium tartrate 0.4 M -
NA
200.0 μl of Potassium Sodium tartrate (2 M)
800.0 μl of water (100 v/v)
F7
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
F8
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of tri-Sodium citrate (5 M)
620.0 μl of water (100 v/v)
F9
  • tri-Sodium citrate 1.6 M -
NA
800.0 μl of tri-Sodium citrate pH 6.5 (2 M)
200.0 μl of water (100 v/v)
F10
  • Iron (III) chloride 0.01 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Jeffamine® M-600 10 v/v -
pH 5.6
10.0 μl of Iron (III) chloride (1 M)
200.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
F11
  • HEPES 0.1 M pH 7.5
  • Jeffamine® M-600 20 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Jeffamine® M-600 (50 v/v)
500.0 μl of water (100 v/v)
F12
  • Cesium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Jeffamine® M-600 30 v/v -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
G1
  • HEPES 0.1 M pH 7.5
  • Potassium di-hydrogen phosphate 0.8 M -
  • Sodium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium di-hydrogen phosphate (2 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
300.0 μl of water (100 v/v)
G2
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
133.3 μl of Ammonium di-hydrogen phosphate (3 M)
866.7 μl of water (100 v/v)
G3
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
566.7 μl of water (100 v/v)
G4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
666.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
G5
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium formate 2 M -
pH 4.6
400.0 μl of Ammonium formate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G6
  • HEPES 0.1 M pH 7.5
  • Ammonium formate 4 M -
pH 7.5
800.0 μl of Ammonium formate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of water (100 v/v)
G7
  • Ammonium formate 2 M -
NA
400.0 μl of Ammonium formate (5 M)
600.0 μl of water (100 v/v)
G8
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 1 M -
  • Ammonium sulfate 0.5 M -
pH 5.6
166.7 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G9
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Sodium chloride (1 M)
266.7 μl of water (100 v/v)
G10
  • Cobalt (II) chloride 0.01 M -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
600.0 μl of Ammonium sulfate (3 M)
10.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
290.0 μl of water (100 v/v)
G11
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
G12
  • Ammonium sulfate 2 M -
NA
666.7 μl of Ammonium sulfate (3 M)
333.3 μl of water (100 v/v)
H1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
H2
  • Potassium Sodium tartrate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
133.3 μl of water (100 v/v)
H3
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
800.0 μl of water (100 v/v)
H4
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
H5
  • BICINE 0.1 M pH 9.5
  • Magnesium chloride 2 M -
pH 9.5
100.0 μl of BICINE pH 9.5 (1 M)
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of water (100 v/v)
H6
  • Imidazole 0.1 M pH 6.5
  • Sodium acetate 1 M -
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
400.0 μl of Sodium acetate (2.5 M)
500.0 μl of water (100 v/v)
H7
  • Cadmium sulfate 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1 M -
pH 7.5
50.0 μl of Cadmium sulfate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Sodium acetate (2.5 M)
450.0 μl of water (100 v/v)
H8
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
560.0 μl of Sodium acetate (2.5 M)
340.0 μl of water (100 v/v)
H9
  • Cetyltrimethylammonium bromide 0.01 M -
  • Magnesium chloride 0.01 M -
  • Sodium chloride 0.5 M -
NA
200.0 μl of Cetyltrimethylammonium bromide (0.05 M)
4.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium chloride (5 M)
696.0 μl of water (100 v/v)
H10
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
H11
  • Sodium di-hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
  • Potassium di-hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
400.0 μl of Sodium chloride (5 M)
25.0 μl of Sodium di-hydrogen phosphate (4 M)
425.0 μl of water (100 v/v)
H12
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)