C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 01

Classics / Classic 01

Created 2009-11-16.

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Classic 01

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Classic 01

Screen Structure

Classic 01

Well Count	24
Number of distinct ingredients	19
Internal diversity (score mean dist)	0.889

Screen Composition

More information about screen composition chart.

ingredient type	Classic 01
Buffer	26
Organic (non-volatile)	12
Polymer	24
Salt	20

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 01 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Calcium chloride 0.1 M - Polyethylene glycol 400 15 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Polyethylene glycol 400 (50 w/v) 100 μl of Calcium chloride (1 M) 500 μl of water (100 v/v)
A2	MES 0.1 M pH 6.5 Polyethylene glycol 400 15 w/v - 🔍	pH 6.5	300 μl of Polyethylene glycol 400 (50 w/v) 100 μl of MES pH 6.5 (1 M) 600 μl of water (100 v/v)
A3	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 15 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 400 (50 w/v) 520 μl of water (100 v/v)
A4	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 15 w/v - 🔍	pH 8.5	300 μl of Polyethylene glycol 400 (50 w/v) 100 μl of tri-Sodium citrate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
A5	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 25 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 40 μl of Magnesium chloride (2.5 M) 500 μl of Polyethylene glycol 400 (50 w/v) 360 μl of water (100 v/v)
A6	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 25 w/v - 🔍	pH 8.5	500 μl of Polyethylene glycol 400 (50 w/v) 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 333 μl of water (100 v/v)
B1	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 28 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 560 μl of Polyethylene glycol 400 (50 w/v) 200 μl of Calcium chloride (1 M) 140 μl of water (100 v/v)
B2	Calcium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 30 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 600 μl of Polyethylene glycol 400 (50 w/v) 100 μl of Calcium chloride (1 M) 200 μl of water (100 v/v)
B3	Sodium acetate 0.1 M - MES 0.1 M pH 6.5 Polyethylene glycol 400 30 w/v - 🔍	pH 6.5	600 μl of Polyethylene glycol 400 (50 w/v) 100 μl of MES pH 6.5 (1 M) 50 μl of Sodium acetate (2 M) 250 μl of water (100 v/v)
B4	Magnesium chloride 0.1 M - MES 0.1 M pH 6.5 Polyethylene glycol 400 30 w/v - 🔍	pH 6.5	40 μl of Magnesium chloride (2.5 M) 600 μl of Polyethylene glycol 400 (50 w/v) 100 μl of MES pH 6.5 (1 M) 260 μl of water (100 v/v)
B5	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 30 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 600 μl of Polyethylene glycol 400 (50 w/v) 220 μl of water (100 v/v)
B6	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 400 (50 w/v) 100 μl of tri-Sodium citrate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of water (100 v/v)
C1	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 30 w/v - 🔍	pH 9	600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 100 μl of BICINE pH 9 (1 M) 100 μl of Sodium chloride (1 M) 200 μl of water (100 v/v)
C2	Zinc sulfate 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 25 w/v - 🔍	pH 6.5	500 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 10 μl of Zinc sulfate (1 M) 100 μl of MES pH 6.5 (1 M) 390 μl of water (100 v/v)
C3	HEPES 0.1 M pH 7.5 Polyethylene glycol 1,000 25 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 500 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v)
C4	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 1,000 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 1,500 (50 w/v) 700 μl of water (100 v/v)
C6	HEPES 0.1 M pH 7.5 Polyethylene glycol 1,500 20 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 1,500 (50 w/v) 500 μl of water (100 v/v)
D1	Polyethylene glycol 1,500 30 w/v - 🔍	NA	600 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
D2	Nickel (II) chloride 0.01 M - Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	10 μl of Nickel (II) chloride (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 490 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 25 w/v - 🔍	NA	500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500 μl of water (100 v/v)
D4	Sodium acetate 0.1 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	pH 6.5	600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 50 μl of Sodium acetate (2 M) 250 μl of water (100 v/v)
D5	Sodium acetate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 3,000 20 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 100 μl of Sodium acetate (2 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 400 μl of water (100 v/v)
D6	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,000 30 w/v - 🔍	pH 8.5	66 μl of Lithium sulfate (3 M) 600 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)