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Supplemental Information

Screen Description

Classics / Classic 01
Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count 24
Number of distinct ingredients 19
Internal diversity (score mean dist) 0.889

Screen Composition

2025-10-22T00:23:01.378647 image/svg+xml Matplotlib v3.10.6, https://matplotlib.org/
ingredient type Classic 01
Buffer 26
Organic (non-volatile) 12
Polymer 24
Salt 20

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic 01 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Calcium chloride 0.1 M -
  • Polyethylene glycol 400 15 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Calcium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
A2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 15 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (50 w/v)
600.0 μl of water (100 v/v)
A3
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 15 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (50 w/v)
520.0 μl of water (100 v/v)
A4
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of tri-Sodium citrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
A5
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 25 w/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
360.0 μl of water (100 v/v)
A6
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 25 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
500.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
B1
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 w/v -
pH 7.5
200.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 400 (50 w/v)
140.0 μl of water (100 v/v)
B2
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 w/v -
pH 4.6
100.0 μl of Calcium chloride (1 M)
600.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
200.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 400 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
B4
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 w/v -
pH 6.5
40.0 μl of Magnesium chloride (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 400 (50 w/v)
260.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 400 (50 w/v)
220.0 μl of water (100 v/v)
B6
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 400 (50 w/v)
100.0 μl of tri-Sodium citrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C1
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 30 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
100.0 μl of Sodium chloride (1 M)
200.0 μl of water (100 v/v)
C2
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
10.0 μl of Zinc sulfate (1 M)
390.0 μl of water (100 v/v)
C3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of water (100 v/v)
C4
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 1,500 15 w/v -
NA
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
700.0 μl of water (100 v/v)
C6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,500 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
D2
  • Nickel (II) chloride 0.01 M -
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
10.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
D3
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
D4
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
D5
  • Sodium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
400.0 μl of water (100 v/v)
D6
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,000 30 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)