C6 - Classic 01 - Screen Contents

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Supplemental Information

Screen Description

JB Classics / Classic 01

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	19
Internal diversity (Score mean dist)	0.89
Internal diversity (Ion mean dist)	0.87
Internal diversity (PEG mean dist)	0.86
Internal diversity (Known pH mean dist)	0.79
Internal diversity (Penalty mean dist)	0.87

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Classic 01

Buffer 26

Organic (non-volatile) 12

Polymer 24

Salt 20

ingredient type	Classic 01
Buffer	26
Organic (non-volatile)	12
Polymer	24
Salt	20

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 01 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 400 15 w/v - Calcium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Calcium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v)
A2	Polyethylene glycol 400 15 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 400 (50 w/v) 600.0 μl of water (100 v/v)
A3	Polyethylene glycol 400 15 w/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (50 w/v) 520.0 μl of water (100 v/v)
A4	Polyethylene glycol 400 15 w/v - tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of tri-Sodium citrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
A5	Polyethylene glycol 400 25 w/v - Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	40.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 360.0 μl of water (100 v/v)
A6	Polyethylene glycol 400 25 w/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	66.67 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.33 μl of water (100 v/v)
B1	Polyethylene glycol 400 28 w/v - Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 400 (50 w/v) 140.0 μl of water (100 v/v)
B2	Polyethylene glycol 400 30 w/v - Calcium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Calcium chloride (1 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of water (100 v/v)
B3	Polyethylene glycol 400 30 w/v - Sodium acetate 0.1 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 50.0 μl of Sodium acetate (2 M) 250.0 μl of water (100 v/v)
B4	Polyethylene glycol 400 30 w/v - Magnesium chloride 0.1 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	40.0 μl of Magnesium chloride (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 260.0 μl of water (100 v/v)
B5	Polyethylene glycol 400 30 w/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 220.0 μl of water (100 v/v)
B6	Polyethylene glycol 400 30 w/v - tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	600.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of tri-Sodium citrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v)
C1	Polyethylene glycol monomethyl ether 550 30 w/v - Sodium chloride 0.1 M - BICINE 0.1 M pH 9 🔍 ⚖️	pH 9	100.0 μl of BICINE pH 9 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 100.0 μl of Sodium chloride (1 M) 200.0 μl of water (100 v/v)
C2	Polyethylene glycol monomethyl ether 550 25 w/v - Zinc sulfate 0.01 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 10.0 μl of Zinc sulfate (1 M) 390.0 μl of water (100 v/v)
C3	Polyethylene glycol 1,000 25 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of water (100 v/v)
C4	Polyethylene glycol 1,000 30 w/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	600.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 15 w/v - 🔍 ⚖️	NA	300.0 μl of Polyethylene glycol 1,500 (50 w/v) 700.0 μl of water (100 v/v)
C6	Polyethylene glycol 1,500 20 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v)
D1	Polyethylene glycol 1,500 30 w/v - 🔍 ⚖️	NA	600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
D2	Polyethylene glycol monomethyl ether 2,000 20 w/v - Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	10.0 μl of Nickel (II) chloride (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 25 w/v - 🔍 ⚖️	NA	500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of water (100 v/v)
D4	Polyethylene glycol monomethyl ether 2,000 30 w/v - Sodium acetate 0.1 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 250.0 μl of water (100 v/v)
D5	Polyethylene glycol 3,000 20 w/v - Sodium acetate 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 400.0 μl of water (100 v/v)
D6	Polyethylene glycol 3,000 30 w/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	66.67 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.33 μl of water (100 v/v)