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Supplemental Information

Screen Description

Classic 02

Classics / Classic 02
Created 2009-11-16.

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Classic 02

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Classic 02

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Screen Structure

Classic 02

Well Count 24
Number of distinct ingredients 9
Internal diversity (score mean dist) 0.683

Screen Composition

More information about screen composition chart.
2025-08-16T06:25:41.917542 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic 02
Buffer 25
Polymer 24
Salt 20

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic 02 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 4 w/v -
pH 4.6
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
820.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 4,000 8 w/v -
NA
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
840.0 μl of water (100 v/v)
A3
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
A4
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 10 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
620.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
610.0 μl of water (100 v/v)
A6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
B1
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
513.3 μl of water (100 v/v)
B2
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
480.0 μl of water (100 v/v)
B3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B4
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 18 w/v -
pH 4.6
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
540.0 μl of water (100 v/v)
B5
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
433.3 μl of water (100 v/v)
B6
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
200.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
410.0 μl of water (100 v/v)
C2
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C3
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 25 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
320.0 μl of water (100 v/v)
C4
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
200.0 μl of Calcium chloride (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
C6
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 30 w/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
260.0 μl of water (100 v/v)
D1
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
D2
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 30 w/v -
pH 7.5
200.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of water (100 v/v)
D3
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
D4
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
D5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 4,000 35 w/v -
NA
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)