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Screen Description

Classic 02

Classics / Classic 02
Created 2009-11-16.

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Classic 02

    Screen Structure

    Classic 02

    Well Count 24
    Number of distinct ingredients 9
    Internal diversity (score mean dist) 0.683

    Screen Composition

    2025-06-15T02:23:28.302482 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classic 02
    Buffer 25
    Polymer 24
    Salt 20

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classic 02 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 4 w/v -
    pH 4.6
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    820 μl of water (100 v/v)
    A2
    • Polyethylene glycol 4,000 8 w/v -
    NA
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    840 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    A4
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 6.5
    80 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    620 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    50 μl of Sodium acetate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    610 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    660 μl of water (100 v/v)
    B1
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    513 μl of water (100 v/v)
    B2
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    100 μl of Sodium acetate (2 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    480 μl of water (100 v/v)
    B3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 18 w/v -
    pH 4.6
    360 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    540 μl of water (100 v/v)
    B5
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    433 μl of water (100 v/v)
    B6
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Calcium chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    C1
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 7.5
    50 μl of Sodium acetate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    410 μl of water (100 v/v)
    C2
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    C3
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 6.5
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    320 μl of water (100 v/v)
    C4
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Calcium chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    C5
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    pH 4.6
    40 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    260 μl of water (100 v/v)
    D1
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    D2
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Calcium chloride (1 M)
    100 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    D4
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    100 μl of Sodium acetate (2 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    D5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 35 w/v -
    NA
    700 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)