C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 04

Classics / Classic 04

Created 2009-11-16.

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Classic 04

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Classic 04

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Screen Structure

Classic 04

Well Count	24
Number of distinct ingredients	22
Internal diversity (score mean dist)	0.888

Screen Composition

More information about screen composition chart.

ingredient type	Classic 04
Buffer	17
Cryoprotectant	1
Organic (non-volatile)	2
Polymer	25
Salt	22

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 04 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 5,000 25 w/v - 🔍	pH 8.5	66.7 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v)
A2	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v)
A3	Potassium chloride 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 6,000 3 w/v - 🔍	pH 8.5	60.0 μl of Polyethylene glycol 6,000 (50 w/v) 20.0 μl of Potassium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 820.0 μl of water (100 v/v)
A4	Magnesium chloride 0.01 M - Polyethylene glycol 6,000 10 w/v - 🔍	NA	4.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 796.0 μl of water (100 v/v)
A5	Sodium chloride 2 M - Polyethylene glycol 6,000 12 w/v - 🔍	NA	240.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v)
A6	Glycerol 5 w/v - Polyethylene glycol 6,000 15 w/v - 🔍	NA	100.0 μl of Glycerol (50 w/v) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 600.0 μl of water (100 v/v)
B1	Magnesium chloride 0.01 M - Potassium chloride 0.05 M - Polyethylene glycol 6,000 15 w/v - 🔍	NA	4.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 10.0 μl of Potassium chloride (5 M) 686.0 μl of water (100 v/v)
B2	tri-Sodium citrate 0.01 M - Polyethylene glycol 6,000 16 w/v - 🔍	NA	320.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of tri-Sodium citrate (2 M) 675.0 μl of water (100 v/v)
B3	Imidazole 0.05 M pH 8 Polyethylene glycol 6,000 20 w/v - 🔍	pH 8	50.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 550.0 μl of water (100 v/v)
B4	Lithium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 25 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Lithium chloride (5 M) 500.0 μl of Polyethylene glycol 6,000 (50 w/v) 380.0 μl of water (100 v/v)
B5	tris chloride BCC 0.1 M pH 8.5 Lithium chloride 0.5 M - Polyethylene glycol 6,000 28 w/v - 🔍	pH 8.5	100.0 μl of Lithium chloride (5 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 240.0 μl of water (100 v/v)
B6	Sodium acetate 0.1 M - Lithium chloride 1 M - Polyethylene glycol 6,000 30 w/v - 🔍	NA	200.0 μl of Lithium chloride (5 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 150.0 μl of water (100 v/v)
C1	tri-Sodium citrate 0.01 M - Polyethylene glycol 6,000 33 w/v - 🔍	NA	660.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of tri-Sodium citrate (2 M) 335.0 μl of water (100 v/v)
C2	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	166.7 μl of Lithium sulfate (3 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 793.3 μl of water (100 v/v)
C3	Lithium sulfate 1 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	333.3 μl of Lithium sulfate (3 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 626.7 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 4 w/v - 🔍	NA	80.0 μl of Polyethylene glycol 8,000 (50 w/v) 920.0 μl of water (100 v/v)
C5	Magnesium sulfate 0.05 M - Lithium chloride 0.2 M - Polyethylene glycol 8,000 8 w/v - 🔍	NA	40.0 μl of Lithium chloride (5 M) 50.0 μl of Magnesium sulfate (1 M) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 750.0 μl of water (100 v/v)
C6	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	160.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 740.0 μl of water (100 v/v)
D1	Zinc acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 600.0 μl of water (100 v/v)
D2	Calcium acetate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 7.5	100.0 μl of Calcium acetate (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)
D3	Sodium acetate 0.1 M - Magnesium acetate 0.05 M - Polyethylene glycol 8,000 10 w/v - 🔍	NA	25.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 725.0 μl of water (100 v/v)
D4	Magnesium acetate 0.2 M - Polyethylene glycol 8,000 10 w/v - 🔍	NA	100.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v)
D5	HEPES 0.1 M pH 7.5 Ethylene glycol 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	pH 7.5	200.0 μl of Ethylene glycol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
D6	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)