C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 04

Classics / Classic 04

Created 2009-11-16.

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Classic 04

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Classic 04

Screen Structure

Classic 04

Well Count	24
Number of distinct ingredients	22
Internal diversity (score mean dist)	0.888

Screen Composition

More information about screen composition chart.

ingredient type	Classic 04
Buffer	17
Cryoprotectant	1
Organic (non-volatile)	2
Polymer	25
Salt	22

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 04 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 5,000 25 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 333 μl of water (100 v/v)
A2	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 100 μl of MES pH 6.5 (1 M) 233 μl of water (100 v/v)
A3	Potassium chloride 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 6,000 3 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 60 μl of Polyethylene glycol 6,000 (50 w/v) 20 μl of Potassium chloride (5 M) 820 μl of water (100 v/v)
A4	Magnesium chloride 0.01 M - Polyethylene glycol 6,000 10 w/v - 🔍	NA	4 μl of Magnesium chloride (2.5 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 796 μl of water (100 v/v)
A5	Sodium chloride 2 M - Polyethylene glycol 6,000 12 w/v - 🔍	NA	400 μl of Sodium chloride (5 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 360 μl of water (100 v/v)
A6	Glycerol 5 w/v - Polyethylene glycol 6,000 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Glycerol (50 w/v) 600 μl of water (100 v/v)
B1	Magnesium chloride 0.01 M - Potassium chloride 0.05 M - Polyethylene glycol 6,000 15 w/v - 🔍	NA	4 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 6,000 (50 w/v) 10 μl of Potassium chloride (5 M) 686 μl of water (100 v/v)
B2	tri-Sodium citrate 0.01 M - Polyethylene glycol 6,000 16 w/v - 🔍	NA	5 μl of tri-Sodium citrate (2 M) 320 μl of Polyethylene glycol 6,000 (50 w/v) 675 μl of water (100 v/v)
B3	Imidazole 0.05 M pH 8 Polyethylene glycol 6,000 20 w/v - 🔍	pH 8	50 μl of Imidazole pH 8 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 550 μl of water (100 v/v)
B4	Lithium chloride 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 25 w/v - 🔍	pH 7.5	20 μl of Lithium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 500 μl of Polyethylene glycol 6,000 (50 w/v) 380 μl of water (100 v/v)
B5	tris chloride BCC 0.1 M pH 8.5 Lithium chloride 0.5 M - Polyethylene glycol 6,000 28 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Lithium chloride (5 M) 560 μl of Polyethylene glycol 6,000 (50 w/v) 240 μl of water (100 v/v)
B6	Sodium acetate 0.1 M - Lithium chloride 1 M - Polyethylene glycol 6,000 30 w/v - 🔍	NA	200 μl of Lithium chloride (5 M) 50 μl of Sodium acetate (2 M) 600 μl of Polyethylene glycol 6,000 (50 w/v) 150 μl of water (100 v/v)
C1	tri-Sodium citrate 0.01 M - Polyethylene glycol 6,000 33 w/v - 🔍	NA	5 μl of tri-Sodium citrate (2 M) 660 μl of Polyethylene glycol 6,000 (50 w/v) 335 μl of water (100 v/v)
C2	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	40 μl of Polyethylene glycol 8,000 (50 w/v) 166 μl of Lithium sulfate (3 M) 793 μl of water (100 v/v)
C3	Lithium sulfate 1 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	40 μl of Polyethylene glycol 8,000 (50 w/v) 333 μl of Lithium sulfate (3 M) 626 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 4 w/v - 🔍	NA	80 μl of Polyethylene glycol 8,000 (50 w/v) 920 μl of water (100 v/v)
C5	Magnesium sulfate 0.05 M - Lithium chloride 0.2 M - Polyethylene glycol 8,000 8 w/v - 🔍	NA	40 μl of Lithium chloride (5 M) 50 μl of Magnesium sulfate (1 M) 160 μl of Polyethylene glycol 8,000 (50 w/v) 750 μl of water (100 v/v)
C6	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 160 μl of Polyethylene glycol 8,000 (50 w/v) 740 μl of water (100 v/v)
D1	Zinc acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 6.5	200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Zinc acetate (2 M) 100 μl of MES pH 6.5 (1 M) 600 μl of water (100 v/v)
D2	Calcium acetate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 100 μl of Calcium acetate (2 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v)
D3	Sodium acetate 0.1 M - Magnesium acetate 0.05 M - Polyethylene glycol 8,000 10 w/v - 🔍	NA	25 μl of Magnesium acetate (2 M) 50 μl of Sodium acetate (2 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 725 μl of water (100 v/v)
D4	Magnesium acetate 0.2 M - Polyethylene glycol 8,000 10 w/v - 🔍	NA	100 μl of Magnesium acetate (2 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v)
D5	HEPES 0.1 M pH 7.5 Ethylene glycol 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	pH 7.5	200 μl of Ethylene glycol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v)
D6	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200 μl of Polyethylene glycol 1,000 (50 w/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v)