C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 05

Classics / Classic 05

Created 2009-11-16.

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Classic 05

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Classic 05

Screen Structure

Classic 05

Well Count	24
Number of distinct ingredients	21
Internal diversity (score mean dist)	0.871

Screen Composition

More information about screen composition chart.

ingredient type	Classic 05
Buffer	22
Cryoprotectant	3
Organic (non-volatile)	3
Organic (volatile)	1
Polymer	24
Salt	17

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 05 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Potassium chloride 0.1 M - Glycerol 5 w/v - Polyethylene glycol 8,000 12 w/v - 🔍	NA	240 μl of Polyethylene glycol 8,000 (50 w/v) 20 μl of Potassium chloride (5 M) 100 μl of Glycerol (50 w/v) 640 μl of water (100 v/v)
A2	Potassium chloride 0.5 M - Glycerol 10 w/v - Polyethylene glycol 8,000 12 w/v - 🔍	NA	240 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Potassium chloride (5 M) 200 μl of Glycerol (50 w/v) 460 μl of water (100 v/v)
A3	Ammonium sulfate 0.2 M - Polyethylene glycol 8,000 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 633 μl of water (100 v/v)
A4	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 15 w/v - 🔍	NA	166 μl of Lithium sulfate (3 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 533 μl of water (100 v/v)
A5	Sodium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	300 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 100 μl of Sodium acetate (2 M) 500 μl of water (100 v/v)
A6	tri-Sodium citrate 0.1 M - Ammonium sulfate 0.05 M - Polyethylene glycol 8,000 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 8,000 (50 w/v) 16 μl of Ammonium sulfate (3 M) 50 μl of tri-Sodium citrate (2 M) 633 μl of water (100 v/v)
B1	Calcium acetate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 7.5	100 μl of Calcium acetate (2 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 440 μl of water (100 v/v)
B2	HEPES 0.1 M pH 7.5 Sodium acetate 0.1 M - 2-Propanol 2 w/v - Polyethylene glycol 8,000 18 w/v - 🔍	pH 7.5	360 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium acetate (2 M) 40 μl of 2-Propanol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v)
B3	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 8.5	66 μl of Lithium sulfate (3 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 473 μl of water (100 v/v)
B4	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 7.5	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)
B5	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 6.5	100 μl of Magnesium acetate (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 400 μl of water (100 v/v)
B6	CHES 0.1 M pH 9.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 9.5	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of CHES pH 9.5 (1 M) 500 μl of water (100 v/v)
C1	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 22 w/v - 🔍	pH 6.5	440 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 100 μl of MES pH 6.5 (1 M) 393 μl of water (100 v/v)
C2	Lithium chloride 0.2 M - Polyethylene glycol 8,000 25 w/v - 🔍	NA	500 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Lithium chloride (5 M) 460 μl of water (100 v/v)
C3	Ammonium sulfate 0.2 M - Polyethylene glycol 8,000 30 w/v - 🔍	NA	600 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 333 μl of water (100 v/v)
C4	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 10,000 8 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 10,000 (50 w/v) 740 μl of water (100 v/v)
C5	Imidazole 0.1 M pH 8 Polyethylene glycol 10,000 14 w/v - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 280 μl of Polyethylene glycol 10,000 (50 w/v) 620 μl of water (100 v/v)
C6	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 10,000 16 w/v - 🔍	pH 8.5	320 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 580 μl of water (100 v/v)
D1	tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.1 M - Glycerol 20 w/v - Polyethylene glycol 10,000 18 w/v - 🔍	pH 8.5	100 μl of Sodium chloride (1 M) 360 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Glycerol (50 w/v) 40 μl of water (100 v/v)
D2	HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 20 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 500 μl of water (100 v/v)
D3	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 10,000 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v)
D4	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 10 w/v - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 200 μl of Polyethylene glycol 20,000 (50 w/v) 700 μl of water (100 v/v)
D5	Magnesium chloride 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 20,000 17 w/v - 🔍	pH 8.5	40 μl of Magnesium chloride (2.5 M) 340 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 520 μl of water (100 v/v)
D6	Polyethylene glycol 20,000 20 w/v - 🔍	NA	400 μl of Polyethylene glycol 20,000 (50 w/v) 600 μl of water (100 v/v)