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Supplemental Information

Screen Description

Classic 05

Classics / Classic 05
Created 2009-11-16.

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Classic 05

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Classic 05

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Screen Structure

Classic 05

Well Count 24
Number of distinct ingredients 21
Internal diversity (score mean dist) 0.871

Screen Composition

More information about screen composition chart.
2025-08-11T20:30:30.554959 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic 05
Buffer 22
Cryoprotectant 3
Organic (non-volatile) 3
Organic (volatile) 1
Polymer 24
Salt 17

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic 05 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Potassium chloride 0.1 M -
  • Glycerol 5 w/v -
  • Polyethylene glycol 8,000 12 w/v -
NA
100.0 μl of Glycerol (50 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Potassium chloride (5 M)
640.0 μl of water (100 v/v)
A2
  • Potassium chloride 0.5 M -
  • Glycerol 10 w/v -
  • Polyethylene glycol 8,000 12 w/v -
NA
200.0 μl of Glycerol (50 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium chloride (5 M)
460.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
633.3 μl of water (100 v/v)
A4
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
166.7 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
533.3 μl of water (100 v/v)
A5
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 15 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
500.0 μl of water (100 v/v)
A6
  • tri-Sodium citrate 0.1 M -
  • Ammonium sulfate 0.05 M -
  • Polyethylene glycol 8,000 15 w/v -
NA
16.7 μl of Ammonium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of tri-Sodium citrate (2 M)
633.3 μl of water (100 v/v)
B1
  • Calcium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 18 w/v -
pH 7.5
100.0 μl of Calcium acetate (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
440.0 μl of water (100 v/v)
B2
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.1 M -
  • 2-Propanol 2 w/v -
  • Polyethylene glycol 8,000 18 w/v -
pH 7.5
40.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
450.0 μl of water (100 v/v)
B3
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 18 w/v -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
473.3 μl of water (100 v/v)
B4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
B5
  • Magnesium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
B6
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 22 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
440.0 μl of Polyethylene glycol 8,000 (50 w/v)
393.3 μl of water (100 v/v)
C2
  • Lithium chloride 0.2 M -
  • Polyethylene glycol 8,000 25 w/v -
NA
40.0 μl of Lithium chloride (5 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
460.0 μl of water (100 v/v)
C3
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
C4
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 10,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
C5
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 10,000 14 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
280.0 μl of Polyethylene glycol 10,000 (50 w/v)
620.0 μl of water (100 v/v)
C6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 10,000 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
D1
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.1 M -
  • Glycerol 20 w/v -
  • Polyethylene glycol 10,000 18 w/v -
pH 8.5
400.0 μl of Glycerol (50 w/v)
360.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
40.0 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
D3
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 10,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D4
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 10 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
700.0 μl of water (100 v/v)
D5
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 17 w/v -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
340.0 μl of Polyethylene glycol 20,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 20,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 20,000 (50 w/v)
600.0 μl of water (100 v/v)