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Supplemental Information

Screen Description

Classic 06

Classics / Classic 06
Created 2009-11-16.

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Classic 06

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Classic 06

    Screen Structure

    Classic 06

    Well Count 24
    Number of distinct ingredients 14
    Internal diversity (score mean dist) 0.648

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:23:18.285130 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classic 06
    Buffer 15
    Cryoprotectant 3
    Organic (non-volatile) 7
    Organic (volatile) 1
    Polymer 3
    Salt 34

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classic 06 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium sulfate 0.5 M -
    • Lithium sulfate 1 M -
    • tri-Sodium citrate 0.1 M -
    		NA
    20 μl of tri-Sodium citrate (5 M)
    166 μl of Ammonium sulfate (3 M)
    333 μl of Lithium sulfate (3 M)
    480 μl of water (100 v/v)
    A2
    • Ammonium sulfate 1 M -
    		NA
    333 μl of Ammonium sulfate (3 M)
    666 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    333 μl of Ammonium sulfate (3 M)
    566 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 w/v -
    • Ammonium sulfate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of Ammonium sulfate (3 M)
    40 μl of Polyethylene glycol 400 (50 w/v)
    526 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1 M -
    		pH 8.5
    333 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    566 μl of water (100 v/v)
    A6
    • tri-Sodium citrate 0.05 M -
    • 2-Propanol 3 w/v -
    • Ammonium sulfate 1.2 M -
    		NA
    25 μl of tri-Sodium citrate (2 M)
    400 μl of Ammonium sulfate (3 M)
    60 μl of 2-Propanol (50 w/v)
    515 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 15 w/v -
    • Ammonium sulfate 1.5 M -
    		pH 8.5
    300 μl of Glycerol (50 w/v)
    500 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of water (100 v/v)
    B2
    • Lithium chloride 0.5 M -
    • Ammonium sulfate 1.6 M -
    		NA
    533 μl of Ammonium sulfate (3 M)
    100 μl of Lithium chloride (5 M)
    366 μl of water (100 v/v)
    B3
    • Lithium sulfate 1 M -
    • Ammonium sulfate 1.6 M -
    		NA
    533 μl of Ammonium sulfate (3 M)
    333 μl of Lithium sulfate (3 M)
    133 μl of water (100 v/v)
    B4
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    		pH 7.5
    200 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Ammonium sulfate (3 M)
    166 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 2 w/v -
    • Ammonium sulfate 1.6 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Ammonium sulfate (3 M)
    40 μl of Polyethylene glycol 1,000 (50 w/v)
    326 μl of water (100 v/v)
    B6
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Ammonium sulfate (3 M)
    300 μl of water (100 v/v)
    C1
    • Sodium chloride 2 M -
    • Ammonium sulfate 2 M -
    		NA
    400 μl of Ammonium sulfate (5 M)
    400 μl of Sodium chloride (5 M)
    200 μl of water (100 v/v)
    C2
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 5 w/v -
    • Ammonium sulfate 2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    100 μl of Polyethylene glycol 400 (50 w/v)
    133 μl of water (100 v/v)
    C4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    		pH 8.5
    666 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    C5
    • Ammonium sulfate 2.2 M -
    		NA
    733 μl of Ammonium sulfate (3 M)
    266 μl of water (100 v/v)
    C6
    • Glycerol 20 w/v -
    • Ammonium sulfate 2.2 M -
    		NA
    250 μl of Glycerol (80 w/v)
    733 μl of Ammonium sulfate (3 M)
    16 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M -
    • Ammonium sulfate 2.4 M -
    		NA
    50 μl of tri-Sodium citrate (2 M)
    800 μl of Ammonium sulfate (3 M)
    150 μl of water (100 v/v)
    D2
    • 2-methyl-2,4-pentanediol BCC 1 w/v -
    • Ammonium sulfate 3 M -
    		NA
    600 μl of Ammonium sulfate (5 M)
    11 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    388 μl of water (100 v/v)
    D3
    • Glycerol 10 w/v -
    • Ammonium sulfate 3 M -
    		NA
    600 μl of Ammonium sulfate (5 M)
    125 μl of Glycerol (80 w/v)
    275 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 3.5 M -
    		pH 7.5
    700 μl of Ammonium sulfate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    D5
    • MES 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 1 w/v -
    • Ammonium sulfate 3.5 M -
    		pH 6.5
    700 μl of Ammonium sulfate (5 M)
    100 μl of MES pH 6.5 (1 M)
    11 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    188 μl of water (100 v/v)
    D6
    • Ammonium sulfate 3.5 M -
    		NA
    875 μl of Ammonium sulfate (4 M)
    125 μl of water (100 v/v)