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Supplemental Information

Screen Description

Classic 06

Classics / Classic 06
Created 2009-11-16.

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Classic 06

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Classic 06

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Screen Structure

Classic 06

Well Count 24
Number of distinct ingredients 14
Internal diversity (score mean dist) 0.648

Screen Composition

More information about screen composition chart.
2025-08-16T07:31:41.844507 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic 06
Buffer 15
Cryoprotectant 3
Organic (non-volatile) 7
Organic (volatile) 1
Polymer 3
Salt 34

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic 06 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 0.5 M -
  • Lithium sulfate 1 M -
  • tri-Sodium citrate 0.1 M -
NA
166.7 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
20.0 μl of tri-Sodium citrate (5 M)
480.0 μl of water (100 v/v)
A2
  • Ammonium sulfate 1 M -
NA
333.3 μl of Ammonium sulfate (3 M)
666.7 μl of water (100 v/v)
A3
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 1 M -
pH 4.6
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
566.7 μl of water (100 v/v)
A4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 w/v -
  • Ammonium sulfate 1 M -
pH 7.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 400 (50 w/v)
526.7 μl of water (100 v/v)
A5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1 M -
pH 8.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
566.7 μl of water (100 v/v)
A6
  • tri-Sodium citrate 0.05 M -
  • 2-Propanol 3 w/v -
  • Ammonium sulfate 1.2 M -
NA
60.0 μl of 2-Propanol (50 w/v)
400.0 μl of Ammonium sulfate (3 M)
25.0 μl of tri-Sodium citrate (2 M)
515.0 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 15 w/v -
  • Ammonium sulfate 1.5 M -
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
300.0 μl of Glycerol (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
B2
  • Lithium chloride 0.5 M -
  • Ammonium sulfate 1.6 M -
NA
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of Lithium chloride (5 M)
366.7 μl of water (100 v/v)
B3
  • Lithium sulfate 1 M -
  • Ammonium sulfate 1.6 M -
NA
533.3 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
133.3 μl of water (100 v/v)
B4
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Sodium chloride (1 M)
166.7 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 2 w/v -
  • Ammonium sulfate 1.6 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 1,000 (50 w/v)
326.7 μl of water (100 v/v)
B6
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
600.0 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of water (100 v/v)
C1
  • Sodium chloride 2 M -
  • Ammonium sulfate 2 M -
NA
400.0 μl of Ammonium sulfate (5 M)
400.0 μl of Sodium chloride (5 M)
200.0 μl of water (100 v/v)
C2
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C3
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 5 w/v -
  • Ammonium sulfate 2 M -
pH 6.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 400 (50 w/v)
133.3 μl of water (100 v/v)
C4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
C5
  • Ammonium sulfate 2.2 M -
NA
733.3 μl of Ammonium sulfate (3 M)
266.7 μl of water (100 v/v)
C6
  • Glycerol 20 w/v -
  • Ammonium sulfate 2.2 M -
NA
733.3 μl of Ammonium sulfate (3 M)
250.0 μl of Glycerol (80 w/v)
16.7 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M -
  • Ammonium sulfate 2.4 M -
NA
800.0 μl of Ammonium sulfate (3 M)
50.0 μl of tri-Sodium citrate (2 M)
150.0 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 1 w/v -
  • Ammonium sulfate 3 M -
NA
11.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
600.0 μl of Ammonium sulfate (5 M)
388.9 μl of water (100 v/v)
D3
  • Glycerol 10 w/v -
  • Ammonium sulfate 3 M -
NA
600.0 μl of Ammonium sulfate (5 M)
125.0 μl of Glycerol (80 w/v)
275.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 3.5 M -
pH 7.5
700.0 μl of Ammonium sulfate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
D5
  • MES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 1 w/v -
  • Ammonium sulfate 3.5 M -
pH 6.5
11.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
700.0 μl of Ammonium sulfate (5 M)
100.0 μl of MES pH 6.5 (1 M)
188.9 μl of water (100 v/v)
D6
  • Ammonium sulfate 3.5 M -
NA
875.0 μl of Ammonium sulfate (4 M)
125.0 μl of water (100 v/v)