C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 07

Classics / Classic 07

Created 2009-11-16.

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Classic 07

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Classic 07

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Screen Structure

Classic 07

Well Count	24
Number of distinct ingredients	18
Internal diversity (score mean dist)	0.730

Screen Composition

More information about screen composition chart.

ingredient type	Classic 07
Buffer	25
Cryoprotectant	1
Organic (non-volatile)	26
Organic (volatile)	1
Polymer	3
Salt	17

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 07 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 10 w/v - 🔍	pH 7.5	111.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of tri-Sodium citrate (2 M) 738.9 μl of water (100 v/v)
A2	Magnesium chloride 0.05 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 12 w/v - 🔍	pH 8.5	133.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 746.7 μl of water (100 v/v)
A3	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 4.6	166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20.0 μl of Calcium chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 713.3 μl of water (100 v/v)
A4	Imidazole 0.1 M pH 8 Polyethylene glycol 4,000 5 w/v - 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 8	166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of Imidazole pH 8 (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 633.3 μl of water (100 v/v)
A5	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 5.6	166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 40.0 μl of Ammonium acetate (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 693.3 μl of water (100 v/v)
A6	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 6.5	166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 633.3 μl of water (100 v/v)
B1	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 7.5	166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 633.3 μl of water (100 v/v)
B2	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	pH 7.5	222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of tri-Sodium citrate (2 M) 627.8 μl of water (100 v/v)
B3	Imidazole 0.1 M pH 8 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	pH 8	222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of Imidazole pH 8 (1 M) 677.8 μl of water (100 v/v)
B4	Sodium chloride 0.2 M - Glycerol 4 w/v - 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	NA	222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 80.0 μl of Glycerol (50 w/v) 200.0 μl of Sodium chloride (1 M) 497.8 μl of water (100 v/v)
B5	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 4.6	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20.0 μl of Calcium chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 546.7 μl of water (100 v/v)
B6	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 5.6	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 40.0 μl of Ammonium acetate (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 526.7 μl of water (100 v/v)
C1	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 6.5	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 466.7 μl of water (100 v/v)
C2	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 166.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of water (100 v/v)
C3	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 466.7 μl of water (100 v/v)
C4	HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 5 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 466.7 μl of water (100 v/v)
C5	Imidazole 0.1 M pH 8 Polyethylene glycol 4,000 10 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 8	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 366.7 μl of water (100 v/v)
C6	Ethanol 20 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	NA	333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 303.0 μl of Ethanol (66 w/v) 363.6 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 35 w/v - 🔍	NA	388.9 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 611.1 μl of water (100 v/v)
D2	Imidazole 0.1 M pH 8 2-methyl-2,4-pentanediol BCC 35 w/v - 🔍	pH 8	388.9 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of Imidazole pH 8 (1 M) 511.1 μl of water (100 v/v)
D3	tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 40 w/v - 🔍	pH 8.5	444.4 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 455.6 μl of water (100 v/v)
D4	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 47 w/v - 🔍	pH 7.5	522.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 377.8 μl of water (100 v/v)
D5	2-Methyl-2-propanol 2 w/v - 2-methyl-2,4-pentanediol BCC 47 w/v - 🔍	NA	40.0 μl of 2-Methyl-2-propanol (50 w/v) 522.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 437.8 μl of water (100 v/v)
D6	2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 444.4 μl of water (100 v/v)