C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 07

Classics / Classic 07

Created 2009-11-16.

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Classic 07

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Classic 07

Screen Structure

Classic 07

Well Count	24
Number of distinct ingredients	18
Internal diversity (score mean dist)	0.730

Screen Composition

More information about screen composition chart.

ingredient type	Classic 07
Buffer	25
Cryoprotectant	1
Organic (non-volatile)	26
Organic (volatile)	1
Polymer	3
Salt	17

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 07 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 10 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 111 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 50 μl of tri-Sodium citrate (2 M) 738 μl of water (100 v/v)
A2	Magnesium chloride 0.05 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 12 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 133 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20 μl of Magnesium chloride (2.5 M) 746 μl of water (100 v/v)
A3	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20 μl of Calcium chloride (1 M) 713 μl of water (100 v/v)
A4	Imidazole 0.1 M pH 8 Polyethylene glycol 4,000 5 w/v - 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 8	100 μl of Polyethylene glycol 4,000 (50 w/v) 166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of Imidazole pH 8 (1 M) 633 μl of water (100 v/v)
A5	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 5.6	166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 40 μl of Ammonium acetate (5 M) 693 μl of water (100 v/v)
A6	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 6.5	100 μl of Magnesium acetate (2 M) 166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of MES pH 6.5 (1 M) 633 μl of water (100 v/v)
B1	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 15 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of tri-Sodium citrate (2 M) 633 μl of water (100 v/v)
B2	tri-Sodium citrate 0.1 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 50 μl of tri-Sodium citrate (2 M) 627 μl of water (100 v/v)
B3	Imidazole 0.1 M pH 8 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	pH 8	222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of Imidazole pH 8 (1 M) 677 μl of water (100 v/v)
B4	Sodium chloride 0.2 M - Glycerol 4 w/v - 2-methyl-2,4-pentanediol BCC 20 w/v - 🔍	NA	80 μl of Glycerol (50 w/v) 200 μl of Sodium chloride (1 M) 222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 497 μl of water (100 v/v)
B5	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 20 μl of Calcium chloride (1 M) 546 μl of water (100 v/v)
B6	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 5.6	333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 40 μl of Ammonium acetate (5 M) 526 μl of water (100 v/v)
C1	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 6.5	100 μl of Magnesium acetate (2 M) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of MES pH 6.5 (1 M) 466 μl of water (100 v/v)
C2	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 166 μl of Ammonium sulfate (3 M) 400 μl of water (100 v/v)
C3	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of tri-Sodium citrate (2 M) 466 μl of water (100 v/v)
C4	HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 5 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 100 μl of Polyethylene glycol 4,000 (50 w/v) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 466 μl of water (100 v/v)
C5	Imidazole 0.1 M pH 8 Polyethylene glycol 4,000 10 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	pH 8	200 μl of Polyethylene glycol 4,000 (50 w/v) 333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of Imidazole pH 8 (1 M) 366 μl of water (100 v/v)
C6	Ethanol 20 w/v - 2-methyl-2,4-pentanediol BCC 30 w/v - 🔍	NA	333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 303 μl of Ethanol (66 w/v) 363 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 35 w/v - 🔍	NA	388 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 611 μl of water (100 v/v)
D2	Imidazole 0.1 M pH 8 2-methyl-2,4-pentanediol BCC 35 w/v - 🔍	pH 8	388 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of Imidazole pH 8 (1 M) 511 μl of water (100 v/v)
D3	tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 40 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 444 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 455 μl of water (100 v/v)
D4	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 47 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 522 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 377 μl of water (100 v/v)
D5	2-Methyl-2-propanol 2 w/v - 2-methyl-2,4-pentanediol BCC 47 w/v - 🔍	NA	522 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 40 μl of 2-Methyl-2-propanol (50 w/v) 437 μl of water (100 v/v)
D6	2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 444 μl of water (100 v/v)