C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 08

Classics / Classic 08

Created 2009-11-16.

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Classic 08

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Classic 08

Screen Structure

Classic 08

Well Count	24
Number of distinct ingredients	16
Internal diversity (score mean dist)	0.840

Screen Composition

More information about screen composition chart.

ingredient type	Classic 08
Buffer	20
Cryoprotectant	2
Organic (non-volatile)	13
Organic (volatile)	18
Polymer	3
Salt	15

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 08 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.01 M - Ethanol 15 w/v - 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	227 μl of Ethanol (66 w/v) 5 μl of Sodium acetate (2 M) 555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 212 μl of water (100 v/v)
A2	Sodium acetate 0.05 M - Sodium chloride 0.05 M - 2-Propanol 20 w/v - 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	400 μl of 2-Propanol (50 w/v) 10 μl of Sodium chloride (5 M) 25 μl of Sodium acetate (2 M) 555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 9 μl of water (100 v/v)
A3	Ammonium di-hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	pH 8.5	100 μl of Ammonium di-hydrogen phosphate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 244 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 55 w/v - 🔍	NA	611 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 388 μl of water (100 v/v)
A5	Calcium chloride 0.01 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 60 w/v - 🔍	pH 4.6	5 μl of Calcium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 666 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 228 μl of water (100 v/v)
A6	Sodium acetate 0.02 M - 2-methyl-2,4-pentanediol BCC 60 w/v - 🔍	NA	10 μl of Sodium acetate (2 M) 666 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 323 μl of water (100 v/v)
B1	MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 70 w/v - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 777 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 122 μl of water (100 v/v)
B2	tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 70 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 777 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 122 μl of water (100 v/v)
B3	Calcium chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Methanol 20 w/v - 🔍	pH 8.5	10 μl of Calcium chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Methanol (50 w/v) 490 μl of water (100 v/v)
B4	tris chloride BCC 0.1 M pH 8.5 Ethanol 2 w/v - 🔍	pH 8.5	30 μl of Ethanol (66 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 869 μl of water (100 v/v)
B5	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 5 w/v - Ethanol 5 w/v - 🔍	pH 7.5	75 μl of Ethanol (66 w/v) 100 μl of HEPES pH 7.5 (1 M) 55 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 768 μl of water (100 v/v)
B6	tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.2 M - 2-methyl-2,4-pentanediol BCC 5 w/v - Ethanol 5 w/v - 🔍	pH 8.5	75 μl of Ethanol (66 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Sodium chloride (1 M) 55 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 568 μl of water (100 v/v)
C1	tris chloride BCC 0.1 M pH 8.5 Ethanol 10 w/v - 🔍	pH 8.5	151 μl of Ethanol (66 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 748 μl of water (100 v/v)
C2	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 4 w/v - Ethanol 12 w/v - 🔍	pH 4.6	181 μl of Ethanol (66 w/v) 80 μl of Polyethylene glycol 400 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 638 μl of water (100 v/v)
C3	tris chloride BCC 0.1 M pH 8.5 Glycerol 5 w/v - Ethanol 14 w/v - 🔍	pH 8.5	212 μl of Ethanol (66 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Glycerol (50 w/v) 587 μl of water (100 v/v)
C4	tris chloride BCC 0.1 M pH 8.5 Ethanol 18 w/v - 🔍	pH 8.5	272 μl of Ethanol (66 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 627 μl of water (100 v/v)
C5	Ethanol 20 w/v - 🔍	NA	303 μl of Ethanol (66 w/v) 697 μl of water (100 v/v)
C6	Glycerol 10 w/v - Ethanol 20 w/v - 🔍	NA	303 μl of Ethanol (66 w/v) 200 μl of Glycerol (50 w/v) 497 μl of water (100 v/v)
D1	Sodium acetate 0.1 M - Polyethylene glycol 6,000 10 w/v - Ethanol 30 w/v - 🔍	NA	454 μl of Ethanol (66 w/v) 50 μl of Sodium acetate (2 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 295 μl of water (100 v/v)
D2	Ethanol 45 w/v - 🔍	NA	681 μl of Ethanol (66 w/v) 318 μl of water (100 v/v)
D3	Sodium acetate 0.01 M - Ethanol 50 w/v - 🔍	NA	757 μl of Ethanol (66 w/v) 5 μl of Sodium acetate (2 M) 237 μl of water (100 v/v)
D4	Sodium acetate 0.05 M - Polyethylene glycol 6,000 1.5 w/v - Ethanol 60 w/v - 🔍	NA	909 μl of Ethanol (66 w/v) 25 μl of Sodium acetate (2 M) 30 μl of Polyethylene glycol 6,000 (50 w/v) 35 μl of water (100 v/v)
D5	Sodium chloride 0.1 M - Ethanol 60 w/v - 🔍	NA	909 μl of Ethanol (66 w/v) 20 μl of Sodium chloride (5 M) 70 μl of water (100 v/v)
D6	Magnesium sulfate 0.01 M - tris chloride BCC 0.1 M pH 8.5 2-Propanol 2 w/v - 🔍	pH 8.5	40 μl of 2-Propanol (50 w/v) 10 μl of Magnesium sulfate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 850 μl of water (100 v/v)