C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 08

Classics / Classic 08

Created 2009-11-16.

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Classic 08

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Classic 08

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Screen Structure

Classic 08

Well Count	24
Number of distinct ingredients	16
Internal diversity (score mean dist)	0.840

Screen Composition

More information about screen composition chart.

ingredient type	Classic 08
Buffer	20
Cryoprotectant	2
Organic (non-volatile)	13
Organic (volatile)	18
Polymer	3
Salt	15

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 08 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.01 M - Ethanol 15 w/v - 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 227.3 μl of Ethanol (66 w/v) 5.0 μl of Sodium acetate (2 M) 212.2 μl of water (100 v/v)
A2	Sodium acetate 0.05 M - Sodium chloride 0.05 M - 2-Propanol 20 w/v - 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	NA	400.0 μl of 2-Propanol (50 w/v) 555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 25.0 μl of Sodium acetate (2 M) 10.0 μl of Sodium chloride (5 M) 9.4 μl of water (100 v/v)
A3	Ammonium di-hydrogen phosphate 0.1 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 w/v - 🔍	pH 8.5	555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of Ammonium di-hydrogen phosphate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 244.4 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 55 w/v - 🔍	NA	611.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 388.9 μl of water (100 v/v)
A5	Calcium chloride 0.01 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 60 w/v - 🔍	pH 4.6	666.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 5.0 μl of Calcium chloride (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 228.3 μl of water (100 v/v)
A6	Sodium acetate 0.02 M - 2-methyl-2,4-pentanediol BCC 60 w/v - 🔍	NA	666.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 10.0 μl of Sodium acetate (2 M) 323.3 μl of water (100 v/v)
B1	MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 70 w/v - 🔍	pH 6.5	777.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of MES pH 6.5 (1 M) 122.2 μl of water (100 v/v)
B2	tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 70 w/v - 🔍	pH 8.5	777.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 122.2 μl of water (100 v/v)
B3	Calcium chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Methanol 20 w/v - 🔍	pH 8.5	10.0 μl of Calcium chloride (1 M) 400.0 μl of Methanol (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v)
B4	tris chloride BCC 0.1 M pH 8.5 Ethanol 2 w/v - 🔍	pH 8.5	30.3 μl of Ethanol (66 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 869.7 μl of water (100 v/v)
B5	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 5 w/v - Ethanol 5 w/v - 🔍	pH 7.5	55.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 75.8 μl of Ethanol (66 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 768.7 μl of water (100 v/v)
B6	tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.2 M - 2-methyl-2,4-pentanediol BCC 5 w/v - Ethanol 5 w/v - 🔍	pH 8.5	55.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 75.8 μl of Ethanol (66 w/v) 200.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 568.7 μl of water (100 v/v)
C1	tris chloride BCC 0.1 M pH 8.5 Ethanol 10 w/v - 🔍	pH 8.5	151.5 μl of Ethanol (66 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 748.5 μl of water (100 v/v)
C2	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 4 w/v - Ethanol 12 w/v - 🔍	pH 4.6	181.8 μl of Ethanol (66 w/v) 80.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 638.2 μl of water (100 v/v)
C3	tris chloride BCC 0.1 M pH 8.5 Glycerol 5 w/v - Ethanol 14 w/v - 🔍	pH 8.5	212.1 μl of Ethanol (66 w/v) 100.0 μl of Glycerol (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 587.9 μl of water (100 v/v)
C4	tris chloride BCC 0.1 M pH 8.5 Ethanol 18 w/v - 🔍	pH 8.5	272.7 μl of Ethanol (66 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 627.3 μl of water (100 v/v)
C5	Ethanol 20 w/v - 🔍	NA	303.0 μl of Ethanol (66 w/v) 697.0 μl of water (100 v/v)
C6	Glycerol 10 w/v - Ethanol 20 w/v - 🔍	NA	303.0 μl of Ethanol (66 w/v) 200.0 μl of Glycerol (50 w/v) 497.0 μl of water (100 v/v)
D1	Sodium acetate 0.1 M - Polyethylene glycol 6,000 10 w/v - Ethanol 30 w/v - 🔍	NA	454.5 μl of Ethanol (66 w/v) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 295.5 μl of water (100 v/v)
D2	Ethanol 45 w/v - 🔍	NA	681.8 μl of Ethanol (66 w/v) 318.2 μl of water (100 v/v)
D3	Sodium acetate 0.01 M - Ethanol 50 w/v - 🔍	NA	757.6 μl of Ethanol (66 w/v) 5.0 μl of Sodium acetate (2 M) 237.4 μl of water (100 v/v)
D4	Sodium acetate 0.05 M - Polyethylene glycol 6,000 1.5 w/v - Ethanol 60 w/v - 🔍	NA	909.1 μl of Ethanol (66 w/v) 30.0 μl of Polyethylene glycol 6,000 (50 w/v) 25.0 μl of Sodium acetate (2 M) 35.9 μl of water (100 v/v)
D5	Sodium chloride 0.1 M - Ethanol 60 w/v - 🔍	NA	909.1 μl of Ethanol (66 w/v) 20.0 μl of Sodium chloride (5 M) 70.9 μl of water (100 v/v)
D6	Magnesium sulfate 0.01 M - tris chloride BCC 0.1 M pH 8.5 2-Propanol 2 w/v - 🔍	pH 8.5	40.0 μl of 2-Propanol (50 w/v) 10.0 μl of Magnesium sulfate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 850.0 μl of water (100 v/v)