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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
c3_4
BCC Core Screens / c3_4
Created 2025-03-07.
Peggy: Mid-High MW, diverse buffers, pH range and salts.
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c3_4
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c3_4
Screen Structure
c3_4
Well Count | 96 |
---|---|
Number of distinct ingredients | 49 |
Internal diversity (score mean dist) | 0.930 |
Screen Composition
ingredient type | c3_4 |
---|---|
Buffer | 90 |
Complex ingredient | 1 |
Cryoprotectant | 1 |
Detergent | 2 |
Organic (non-volatile) | 3 |
Organic (volatile) | 6 |
Polymer | 96 |
Salt | 54 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 6 | 125.0 μl of 2-propanol BCC (80 v/v) 200.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 575.0 μl of water (100 v/v) |
A2 |
| NA | 200.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 49.0 μl of tacsimate BCC pH 7 (100 v/v) 1.0 μl of tacsimate BCC pH 4 (100 v/v) 650.0 μl of water (100 v/v) |
A3 |
| pH 5.5 | 240.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 660.0 μl of water (100 v/v) |
A4 |
| NA | 200.0 μl of cesium chloride BCC (1 M) 300.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 500.0 μl of water (100 v/v) |
A5 |
| pH 5.5 | 360.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 540.0 μl of water (100 v/v) |
A6 |
| pH 8.5 | 360.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 75.0 μl of potassium thiocyanate BCC (2 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 515.0 μl of water (100 v/v) |
A7 |
| NA | 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 10.0 μl of tergitol NP-40 (1 w/v) 590.0 μl of water (100 v/v) |
A8 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 500.0 μl of water (100 v/v) |
A9 |
| pH 7.5 | 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
A10 |
| pH 5.5 | 480.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 420.0 μl of water (100 v/v) |
A11 |
| pH 6.5 | 200.0 μl of ammonium sulfate BCC (1 M) 600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 100.0 μl of water (100 v/v) |
A12 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
B1 |
| pH 5.5 | 720.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 91.4 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 8.6 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 180.0 μl of water (100 v/v) |
B2 |
| pH 9 | 37.1 μl of CHES BCC pH 10 (0.8 M) 87.9 μl of CHES BCC pH 8.3 (0.8 M) 200.0 μl of magnesium formate BCC (1 M) 500.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 175.0 μl of water (100 v/v) |
B3 |
| pH 5.5 | 400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
B4 |
| pH 6 | 37.4 μl of bis-tris chloride BCC pH 6.5 (1 M) 62.6 μl of bis-tris chloride BCC pH 5.5 (1 M) 80.0 μl of lithium acetate BCC (2.5 M) 600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 220.0 μl of water (100 v/v) |
B5 |
| pH 7.5 | 80.0 μl of magnesium acetate BCC (2.5 M) 500.0 μl of polyethylene glycol 6000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
B6 |
| pH 9 | 37.1 μl of CHES BCC pH 10 (0.8 M) 87.9 μl of CHES BCC pH 8.3 (0.8 M) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 475.0 μl of water (100 v/v) |
B7 |
| pH 6 | 160.0 μl of polyethylene glycol 6000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 740.0 μl of water (100 v/v) |
B8 |
| pH 7 | 200.0 μl of polyethylene glycol 6000 BCC (50 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 50.0 μl of sodium chloride BCC (2 M) 650.0 μl of water (100 v/v) |
B9 |
| pH 7.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of polyethylene glycol 6000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 650.0 μl of water (100 v/v) |
B10 |
| pH 7.5 | 100.0 μl of calcium acetate BCC (1 M) 200.0 μl of polyethylene glycol 6000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
B11 |
| NA | 10.0 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v) 300.0 μl of polyethylene glycol 6000 BCC (50 w/v) 690.0 μl of water (100 v/v) |
B12 |
| pH 5 | 300.0 μl of polyethylene glycol 6000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 600.0 μl of water (100 v/v) |
C1 |
| pH 8 | 300.0 μl of polyethylene glycol 6000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 600.0 μl of water (100 v/v) |
C2 |
| pH 8.5 | 300.0 μl of polyethylene glycol 6000 BCC (50 w/v) 75.0 μl of potassium thiocyanate BCC (2 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 575.0 μl of water (100 v/v) |
C3 |
| NA | 10.0 μl of dioxane BCC (100 v/v) 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 590.0 μl of water (100 v/v) |
C4 |
| pH 4.5 | 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (2 M) |
C5 |
| pH 5.5 | 400.0 μl of polyethylene glycol 6000 BCC (50 w/v) 25.0 μl of tert-butanol BCC (100 v/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 475.0 μl of water (100 v/v) |
C6 |
| pH 6.5 | 109.4 μl of ADA BCC pH 5.5 (0.5 M) 90.6 μl of ADA BCC pH 7.5 (0.5 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of polyethylene glycol 6000 BCC (50 w/v) 100.0 μl of water (100 v/v) |
C7 |
| pH 7.5 | 500.0 μl of polyethylene glycol 6000 BCC (50 w/v) 100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M) 400.0 μl of water (100 v/v) |
C8 |
| pH 8.5 | 600.0 μl of polyethylene glycol 6000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
C9 |
| pH 6 | 160.0 μl of polyethylene glycol 8000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 740.0 μl of water (100 v/v) |
C10 |
| pH 8.5 | 160.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 740.0 μl of water (100 v/v) |
C11 |
| pH 5.5 | 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 700.0 μl of water (100 v/v) |
C12 |
| pH 6 | 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 20.0 μl of zinc acetate BCC (1 M) 680.0 μl of water (100 v/v) |
D1 |
| pH 6.2 | 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 20.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M) 80.0 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M) 600.0 μl of water (100 v/v) |
D2 |
| pH 7 | 200.0 μl of magnesium chloride BCC (1 M) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 500.0 μl of water (100 v/v) |
D3 |
| pH 7.5 | 80.0 μl of ethylene glycol BCC (100 v/v) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 620.0 μl of water (100 v/v) |
D4 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 700.0 μl of water (100 v/v) |
D5 |
| pH 8 | 200.0 μl of calcium acetate BCC (1 M) 100.0 μl of imidazole BCC pH 8 (1 M) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 500.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 650.0 μl of water (100 v/v) |
D7 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 200.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 575.0 μl of water (100 v/v) |
D8 |
| pH 5.5 | 20.0 μl of calcium chloride BCC (1 M) 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 7.1 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M) 22.3 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M) 710.6 μl of water (100 v/v) |
D9 |
| pH 6 | 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 50.0 μl of sodium chloride BCC (2 M) 610.0 μl of water (100 v/v) |
D10 |
| pH 6.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 620.0 μl of water (100 v/v) |
D11 |
| pH 7 | 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of potassium chloride BCC (1 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 560.0 μl of water (100 v/v) |
D12 |
| pH 7.5 | 100.0 μl of ammonium sulfate BCC (1 M) 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 560.0 μl of water (100 v/v) |
E1 |
| pH 8 | 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 660.0 μl of water (100 v/v) |
E2 |
| pH 8.5 | 40.0 μl of magnesium acetate BCC (2.5 M) 240.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 620.0 μl of water (100 v/v) |
E3 |
| NA | 500.0 μl of lithium sulfate BCC (1 M) 300.0 μl of polyethylene glycol 8000 BCC (50 w/v) 200.0 μl of water (100 v/v) |
E4 |
| pH 6.5 | 300.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 600.0 μl of water (100 v/v) |
E5 |
| pH 5.5 | 360.0 μl of polyethylene glycol 8000 BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 540.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 200.0 μl of calcium acetate BCC (1 M) 360.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 340.0 μl of water (100 v/v) |
E7 |
| pH 6.5 | 20.0 μl of calcium chloride BCC (1 M) 360.0 μl of polyethylene glycol 8000 BCC (50 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 520.0 μl of water (100 v/v) |
E8 |
| pH 6.5 | 360.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 20.0 μl of zinc acetate BCC (1 M) 520.0 μl of water (100 v/v) |
E9 |
| NA | 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of potassium dihydrogen phosphate BCC (1 M) 550.0 μl of water (100 v/v) |
E10 |
| pH 4.2 | 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 300.0 μl of water (100 v/v) |
E11 |
| pH 6 | 200.0 μl of calcium acetate BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of water (100 v/v) |
E12 |
| pH 6.5 | 80.0 μl of magnesium acetate BCC (2.5 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 420.0 μl of water (100 v/v) |
F1 |
| pH 7.5 | 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
F2 |
| pH 8.5 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
F3 |
| pH 9.4 | 200.0 μl of magnesium chloride BCC (1 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium bicine BCC pH 9.4 (1 M) 300.0 μl of water (100 v/v) |
F4 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 475.0 μl of water (100 v/v) |
F5 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 400.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 300.0 μl of water (100 v/v) |
F6 |
| NA | 200.0 μl of ammonium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 200.0 μl of water (100 v/v) |
F7 |
| pH 5 | 200.0 μl of lithium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 100.0 μl of water (100 v/v) |
F8 |
| pH 6.5 | 200.0 μl of ammonium sulfate BCC (1 M) 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 100.0 μl of water (100 v/v) |
F9 |
| pH 6.5 | 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 200.0 μl of sodium acetate BCC (1 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 100.0 μl of water (100 v/v) |
F10 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 600.0 μl of polyethylene glycol 8000 BCC (50 w/v) 100.0 μl of sodium chloride BCC (2 M) 200.0 μl of water (100 v/v) |
F11 |
| pH 8 | 8.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 192.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 250.0 μl of polyethylene glycol 10000 BCC (30 w/v) 550.0 μl of water (100 v/v) |
F12 |
| pH 4.5 | 300.0 μl of polyethylene glycol 10000 BCC (30 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 600.0 μl of water (100 v/v) |
G1 |
| pH 8 | 8.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 192.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 416.7 μl of polyethylene glycol 10000 BCC (30 w/v) 383.3 μl of water (100 v/v) |
G2 |
| pH 4.5 | 500.0 μl of polyethylene glycol 10000 BCC (30 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 400.0 μl of water (100 v/v) |
G3 |
| pH 5.5 | 20.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 566.7 μl of polyethylene glycol 10000 BCC (30 w/v) 313.3 μl of water (100 v/v) |
G4 |
| pH 8 | 8.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 192.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 583.3 μl of polyethylene glycol 10000 BCC (30 w/v) 216.7 μl of water (100 v/v) |
G5 |
| pH 7.5 | 666.7 μl of polyethylene glycol 10000 BCC (30 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
G6 |
| pH 8 | 8.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 192.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 750.0 μl of polyethylene glycol 10000 BCC (30 w/v) 50.0 μl of water (100 v/v) |
G7 |
| pH 4.5 | 766.7 μl of polyethylene glycol 10000 BCC (30 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 133.3 μl of water (100 v/v) |
G8 |
| pH 4.5 | 900.0 μl of polyethylene glycol 10000 BCC (30 w/v) 15.6 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.4 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 50.0 μl of water (100 v/v) |
G9 |
| pH 6 | 320.0 μl of polyethylene glycol 20000 BCC (25 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 580.0 μl of water (100 v/v) |
G10 |
| pH 9 | 20.0 μl of dioxane BCC (100 v/v) 400.0 μl of polyethylene glycol 20000 BCC (25 w/v) 12.4 μl of sodium bicine BCC pH 7.4 (1 M) 87.6 μl of sodium bicine BCC pH 9.4 (1 M) 480.0 μl of water (100 v/v) |
G11 |
| pH 6.5 | 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 420.0 μl of water (100 v/v) |
G12 |
| pH 6.5 | 100.0 μl of calcium chloride BCC (1 M) 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 320.0 μl of water (100 v/v) |
H1 |
| pH 7 | 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v) |
H2 |
| pH 8 | 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 420.0 μl of water (100 v/v) |
H3 |
| pH 8.5 | 480.0 μl of polyethylene glycol 20000 BCC (25 w/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 420.0 μl of water (100 v/v) |
H4 |
| pH 6 | 300.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 300.0 μl of sodium bromide BCC (1 M) 300.0 μl of water (100 v/v) |
H5 |
| pH 7.4 | 200.0 μl of magnesium formate BCC (1 M) 300.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 100.0 μl of sodium bicine BCC pH 7.4 (1 M) 400.0 μl of water (100 v/v) |
H6 |
| pH 5.5 | 187.5 μl of 2-propanol BCC (80 v/v) 40.0 μl of ammonium acetate BCC (5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 400.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 272.5 μl of water (100 v/v) |
H7 |
| pH 7.5 | 400.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 100.0 μl of tert-butanol BCC (100 v/v) 400.0 μl of water (100 v/v) |
H8 |
| pH 8.5 | 300.0 μl of calcium chloride BCC (1 M) 480.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 40.7 μl of sodium bicine BCC pH 7.4 (1 M) 59.3 μl of sodium bicine BCC pH 9.4 (1 M) 120.0 μl of water (100 v/v) |
H9 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 300.0 μl of sodium nitrate BCC (1 M) 100.0 μl of water (100 v/v) |
H10 |
| NA | 600.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 400.0 μl of water (100 v/v) |
H11 |
| pH 7 | 600.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 200.0 μl of sodium nitrate BCC (1 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 100.0 μl of water (100 v/v) |
H12 |
| pH 5.5 | 700.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 200.0 μl of water (100 v/v) |