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Supplemental Information

Screen Description

Created 2025-03-07.
Peggy: Mid-High MW, diverse buffers, pH range and salts.

Rigaku Recipe XML

μl
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Barcodes

  • Z0002642 Lot 1 (1500.0 µl)
  • Z0002788 Lot 2 (1500.0 µl)
  • Z0002789 Lot 3 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 49
Internal diversity (Score mean dist)0.93
Internal diversity (Ion mean dist)0.91
Internal diversity (PEG mean dist)0.87
Internal diversity (Known pH mean dist)0.79
Internal diversity (Penalty mean dist)0.89

Screen Composition

Chemical classes bar chart
ingredient type c3_4
Buffer 90
Complex ingredient 1
Cryoprotectant 1
Detergent 2
Organic (non-volatile) 3
Organic (volatile) 6
Polymer 96
Salt 54

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_4 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
125.0 μl of 2-propanol BCC (80 v/v)
200.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
69.11 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.89 μl of sodium cacodylate BCC pH 7.3 (1 M)
575.0 μl of water (100 v/v)
A2
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7
  • tacsimate BCC 5 v/v pH 7
NA
200.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of tacsimate BCC pH 7 (100 v/v)
0.0 μl of tacsimate BCC pH 4 (100 v/v)
650.0 μl of water (100 v/v)
A3
  • polyethylene glycol monomethyl ether 5000 BCC 12 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
240.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
91.39 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.61 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
660.0 μl of water (100 v/v)
A4
  • polyethylene glycol monomethyl ether 5000 BCC 15 w/v -
  • cesium chloride BCC 0.2 M -
NA
200.0 μl of cesium chloride BCC (1 M)
300.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
500.0 μl of water (100 v/v)
A5
  • polyethylene glycol monomethyl ether 5000 BCC 18 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
360.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
91.39 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.61 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
540.0 μl of water (100 v/v)
A6
  • polyethylene glycol monomethyl ether 5000 BCC 18 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
360.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
75.0 μl of potassium thiocyanate BCC (2 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
515.0 μl of water (100 v/v)
A7
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • tergitol NP-40 BCC 0.01 w/v -
NA
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
10.0 μl of tergitol NP-40 BCC (1 w/v)
590.0 μl of water (100 v/v)
A8
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
500.0 μl of water (100 v/v)
A9
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
A10
  • polyethylene glycol monomethyl ether 5000 BCC 24 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
480.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
91.39 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.61 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
420.0 μl of water (100 v/v)
A11
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • ammonium sulfate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
100.0 μl of water (100 v/v)
A12
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
B1
  • polyethylene glycol monomethyl ether 5000 BCC 36 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
720.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
91.39 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
8.61 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
180.0 μl of water (100 v/v)
B2
  • polyethylene glycol monomethyl ether 5000 BCC 25 w/v -
  • magnesium formate BCC 0.2 M -
  • CHES BCC 0.1 M pH 9
pH 9
37.12 μl of CHES BCC pH 10.3 (0.8 M)
87.88 μl of CHES BCC pH 8.3 (0.8 M)
200.0 μl of magnesium formate BCC (1 M)
500.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
175.0 μl of water (100 v/v)
B3
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
B4
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • lithium acetate BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 6
pH 6
37.4 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.6 μl of bis-tris chloride BCC pH 5.5 (1 M)
80.0 μl of lithium acetate BCC (2.5 M)
600.0 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
220.0 μl of water (100 v/v)
B5
  • polyethylene glycol 6000 BCC 25 w/v -
  • magnesium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
80.0 μl of magnesium acetate BCC (2.5 M)
500.0 μl of polyethylene glycol 6000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B6
  • polyethylene glycol 6000 BCC 20 w/v -
  • CHES BCC 0.1 M pH 9
pH 9
37.12 μl of CHES BCC pH 10.3 (0.8 M)
87.88 μl of CHES BCC pH 8.3 (0.8 M)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
475.0 μl of water (100 v/v)
B7
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
160.0 μl of polyethylene glycol 6000 BCC (50 w/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
740.0 μl of water (100 v/v)
B8
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
200.0 μl of polyethylene glycol 6000 BCC (50 w/v)
50.0 μl of sodium chloride BCC (2 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
B9
  • polyethylene glycol 6000 BCC 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of polyethylene glycol 6000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
B10
  • polyethylene glycol 6000 BCC 10 w/v -
  • calcium acetate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of calcium acetate BCC (1 M)
200.0 μl of polyethylene glycol 6000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
B11
  • polyethylene glycol 6000 BCC 15 w/v -
  • n-octyl-b-D-Glucopyranoside BCC 0.1 w/v -
NA
10.0 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v)
300.0 μl of polyethylene glycol 6000 BCC (50 w/v)
690.0 μl of water (100 v/v)
B12
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
300.0 μl of polyethylene glycol 6000 BCC (50 w/v)
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600.0 μl of water (100 v/v)
C1
  • polyethylene glycol 6000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of polyethylene glycol 6000 BCC (50 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
600.0 μl of water (100 v/v)
C2
  • polyethylene glycol 6000 BCC 15 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
300.0 μl of polyethylene glycol 6000 BCC (50 w/v)
75.0 μl of potassium thiocyanate BCC (2 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
575.0 μl of water (100 v/v)
C3
  • polyethylene glycol 6000 BCC 20 w/v -
  • dioxane BCC 1 v/v -
NA
10.0 μl of dioxane BCC (100 v/v)
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
590.0 μl of water (100 v/v)
C4
  • polyethylene glycol 6000 BCC 20 w/v -
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (2 M)
C5
  • polyethylene glycol 6000 BCC 20 w/v -
  • tert-butanol BCC 2.5 v/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of polyethylene glycol 6000 BCC (50 w/v)
25.0 μl of tert-butanol BCC (100 v/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
475.0 μl of water (100 v/v)
C6
  • polyethylene glycol 6000 BCC 25 w/v -
  • lithium sulfate BCC 0.2 M -
  • ADA BCC 0.1 M pH 6.5
pH 6.5
109.38 μl of ADA BCC pH 5.5 (0.5 M)
90.62 μl of ADA BCC pH 7.5 (0.5 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of polyethylene glycol 6000 BCC (50 w/v)
100.0 μl of water (100 v/v)
C7
  • polyethylene glycol 6000 BCC 25 w/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of polyethylene glycol 6000 BCC (50 w/v)
100.0 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
C8
  • polyethylene glycol 6000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of polyethylene glycol 6000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
C9
  • polyethylene glycol 8000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
160.0 μl of polyethylene glycol 8000 BCC (50 w/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
740.0 μl of water (100 v/v)
C10
  • polyethylene glycol 8000 BCC 8 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
160.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
740.0 μl of water (100 v/v)
C11
  • polyethylene glycol 8000 BCC 10 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
700.0 μl of water (100 v/v)
C12
  • polyethylene glycol 8000 BCC 10 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
20.0 μl of zinc acetate BCC (1 M)
680.0 μl of water (100 v/v)
D1
  • polyethylene glycol 8000 BCC 10 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
pH 6.2
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium chloride BCC (2 M)
20.24 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
79.76 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
600.0 μl of water (100 v/v)
D2
  • polyethylene glycol 8000 BCC 10 w/v -
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
200.0 μl of magnesium chloride BCC (1 M)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
D3
  • polyethylene glycol 8000 BCC 10 w/v -
  • ethylene glycol BCC 8 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
80.0 μl of ethylene glycol BCC (100 v/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
620.0 μl of water (100 v/v)
D4
  • polyethylene glycol 8000 BCC 10 w/v -
  • imidazole BCC 0.1 M pH 8
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
700.0 μl of water (100 v/v)
D5
  • calcium acetate BCC 0.2 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • imidazole BCC 0.1 M pH 8
pH 8
200.0 μl of calcium acetate BCC (1 M)
100.0 μl of imidazole BCC pH 8 (1 M)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
500.0 μl of water (100 v/v)
D6
  • polyethylene glycol 8000 BCC 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
650.0 μl of water (100 v/v)
D7
  • polyethylene glycol 8000 BCC 10 w/v -
  • sodium chloride BCC 0.2 M -
  • CHES BCC 0.1 M pH 9.5
pH 9.5
79.78 μl of CHES BCC pH 10.3 (0.8 M)
45.22 μl of CHES BCC pH 8.3 (0.8 M)
200.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium chloride BCC (2 M)
575.0 μl of water (100 v/v)
D8
  • polyethylene glycol 8000 BCC 12 w/v -
  • calcium chloride BCC 0.02 M -
  • sodium malonate-malonic acid BCC 0.1 M pH 5.5
pH 5.5
20.0 μl of calcium chloride BCC (1 M)
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
16.35 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M)
13.06 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
710.59 μl of water (100 v/v)
D9
  • polyethylene glycol 8000 BCC 12 w/v -
  • sodium chloride BCC 0.1 M -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
69.11 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.89 μl of sodium cacodylate BCC pH 7.3 (1 M)
50.0 μl of sodium chloride BCC (2 M)
610.0 μl of water (100 v/v)
D10
  • polyethylene glycol 8000 BCC 12 w/v -
  • magnesium acetate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
40.0 μl of magnesium acetate BCC (2.5 M)
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
620.0 μl of water (100 v/v)
D11
  • polyethylene glycol 8000 BCC 12 w/v -
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of potassium chloride BCC (1 M)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
D12
  • polyethylene glycol 8000 BCC 12 w/v -
  • ammonium sulfate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of ammonium sulfate BCC (1 M)
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
E1
  • polyethylene glycol 8000 BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
660.0 μl of water (100 v/v)
E2
  • polyethylene glycol 8000 BCC 12 w/v -
  • magnesium acetate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40.0 μl of magnesium acetate BCC (2.5 M)
240.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
620.0 μl of water (100 v/v)
E3
  • polyethylene glycol 8000 BCC 15 w/v -
  • lithium sulfate BCC 0.5 M -
NA
500.0 μl of lithium sulfate BCC (1 M)
300.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of water (100 v/v)
E4
  • polyethylene glycol 8000 BCC 15 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
300.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
600.0 μl of water (100 v/v)
E5
  • polyethylene glycol 8000 BCC 18 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
360.0 μl of polyethylene glycol 8000 BCC (50 w/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
540.0 μl of water (100 v/v)
E6
  • polyethylene glycol 8000 BCC 18 w/v -
  • calcium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of calcium acetate BCC (1 M)
360.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
340.0 μl of water (100 v/v)
E7
  • polyethylene glycol 8000 BCC 18 w/v -
  • calcium chloride BCC 0.02 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of calcium chloride BCC (1 M)
360.0 μl of polyethylene glycol 8000 BCC (50 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
520.0 μl of water (100 v/v)
E8
  • polyethylene glycol 8000 BCC 18 w/v -
  • zinc acetate BCC 0.02 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
360.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
20.0 μl of zinc acetate BCC (1 M)
520.0 μl of water (100 v/v)
E9
  • polyethylene glycol 8000 BCC 20 w/v -
  • potassium dihydrogen phosphate BCC 0.05 M -
NA
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of potassium dihydrogen phosphate BCC (1 M)
550.0 μl of water (100 v/v)
E10
  • polyethylene glycol 8000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
pH 4.2
86.12 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
113.88 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium chloride BCC (2 M)
300.0 μl of water (100 v/v)
E11
  • polyethylene glycol 8000 BCC 20 w/v -
  • calcium acetate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
200.0 μl of calcium acetate BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
E12
  • polyethylene glycol 8000 BCC 20 w/v -
  • magnesium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
80.0 μl of magnesium acetate BCC (2.5 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
420.0 μl of water (100 v/v)
F1
  • polyethylene glycol 8000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
F2
  • polyethylene glycol 8000 BCC 20 w/v -
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F3
  • polyethylene glycol 8000 BCC 20 w/v -
  • magnesium chloride BCC 0.2 M -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
200.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium bicine BCC pH 9.4 (1 M)
300.0 μl of water (100 v/v)
F4
  • polyethylene glycol 8000 BCC 20 w/v -
  • CHES BCC 0.1 M pH 9.5
pH 9.5
79.78 μl of CHES BCC pH 10.3 (0.8 M)
45.22 μl of CHES BCC pH 8.3 (0.8 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
475.0 μl of water (100 v/v)
F5
  • polyethylene glycol 8000 BCC 20 w/v -
  • sodium chloride BCC 0.2 M -
  • CAPS BCC 0.1 M pH 10.5
pH 10.5
85.99 μl of CAPS BCC pH 9.4 (0.5 M)
114.01 μl of CAPS BCC pH 11.4 (0.5 M)
400.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium chloride BCC (2 M)
300.0 μl of water (100 v/v)
F6
  • polyethylene glycol 8000 BCC 30 w/v -
  • ammonium sulfate BCC 0.2 M -
NA
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of water (100 v/v)
F7
  • polyethylene glycol 8000 BCC 30 w/v -
  • lithium sulfate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of lithium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
100.0 μl of water (100 v/v)
F8
  • polyethylene glycol 8000 BCC 30 w/v -
  • ammonium sulfate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of ammonium sulfate BCC (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
100.0 μl of water (100 v/v)
F9
  • polyethylene glycol 8000 BCC 30 w/v -
  • sodium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
200.0 μl of sodium acetate BCC (1 M)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
100.0 μl of water (100 v/v)
F10
  • polyethylene glycol 8000 BCC 30 w/v -
  • sodium chloride BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
600.0 μl of polyethylene glycol 8000 BCC (50 w/v)
100.0 μl of sodium chloride BCC (2 M)
200.0 μl of water (100 v/v)
F11
  • polyethylene glycol 10000 BCC 7.5 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 8
pH 8
9.11 μl of DL-malate-imidazole BCC pH 2 (1 M)
190.89 μl of DL-malate-imidazole BCC pH 9 (1 M)
250.0 μl of polyethylene glycol 10000 BCC (30 w/v)
550.0 μl of water (100 v/v)
F12
  • polyethylene glycol 10000 BCC 9 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
300.0 μl of polyethylene glycol 10000 BCC (30 w/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600.0 μl of water (100 v/v)
G1
  • polyethylene glycol 10000 BCC 12.5 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 8
pH 8
9.11 μl of DL-malate-imidazole BCC pH 2 (1 M)
190.89 μl of DL-malate-imidazole BCC pH 9 (1 M)
416.67 μl of polyethylene glycol 10000 BCC (30 w/v)
383.33 μl of water (100 v/v)
G2
  • polyethylene glycol 10000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
500.0 μl of polyethylene glycol 10000 BCC (30 w/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
400.0 μl of water (100 v/v)
G3
  • polyethylene glycol 10000 BCC 17 w/v -
  • ammonium acetate BCC 0.1 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
20.0 μl of ammonium acetate BCC (5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
566.67 μl of polyethylene glycol 10000 BCC (30 w/v)
313.33 μl of water (100 v/v)
G4
  • polyethylene glycol 10000 BCC 17.5 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 8
pH 8
9.11 μl of DL-malate-imidazole BCC pH 2 (1 M)
190.89 μl of DL-malate-imidazole BCC pH 9 (1 M)
583.33 μl of polyethylene glycol 10000 BCC (30 w/v)
216.67 μl of water (100 v/v)
G5
  • polyethylene glycol 10000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
666.67 μl of polyethylene glycol 10000 BCC (30 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
233.33 μl of water (100 v/v)
G6
  • polyethylene glycol 10000 BCC 22.5 w/v -
  • DL-malate-imidazole BCC 0.2 M pH 8
pH 8
9.11 μl of DL-malate-imidazole BCC pH 2 (1 M)
190.89 μl of DL-malate-imidazole BCC pH 9 (1 M)
750.0 μl of polyethylene glycol 10000 BCC (30 w/v)
50.0 μl of water (100 v/v)
G7
  • polyethylene glycol 10000 BCC 23 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
766.67 μl of polyethylene glycol 10000 BCC (30 w/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
133.33 μl of water (100 v/v)
G8
  • polyethylene glycol 10000 BCC 27 w/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 4.5
pH 4.5
900.0 μl of polyethylene glycol 10000 BCC (30 w/v)
15.64 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.36 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
50.0 μl of water (100 v/v)
G9
  • polyethylene glycol 20000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
320.0 μl of polyethylene glycol 20000 BCC (25 w/v)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
580.0 μl of water (100 v/v)
G10
  • polyethylene glycol 20000 BCC 10 w/v -
  • dioxane BCC 2 v/v -
  • sodium bicine BCC 0.1 M pH 9
pH 9
20.0 μl of dioxane BCC (100 v/v)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
12.37 μl of sodium bicine BCC pH 7.4 (1 M)
87.63 μl of sodium bicine BCC pH 9.4 (1 M)
480.0 μl of water (100 v/v)
G11
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
420.0 μl of water (100 v/v)
G12
  • polyethylene glycol 20000 BCC 12 w/v -
  • calcium chloride BCC 0.1 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of calcium chloride BCC (1 M)
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
320.0 μl of water (100 v/v)
H1
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
81.75 μl of sodium HEPES BCC pH 6.5 (1 M)
18.25 μl of sodium HEPES BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
H2
  • polyethylene glycol 20000 BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
420.0 μl of water (100 v/v)
H3
  • polyethylene glycol 20000 BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
480.0 μl of polyethylene glycol 20000 BCC (25 w/v)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
420.0 μl of water (100 v/v)
H4
  • polyethylene glycol smear 0.2-10kD BCC 15 w/v -
  • sodium bromide BCC 0.3 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
300.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
300.0 μl of sodium bromide BCC (1 M)
61.63 μl of sodium MES BCC pH 5.2 (1 M)
38.37 μl of sodium MES BCC pH 7.2 (1 M)
300.0 μl of water (100 v/v)
H5
  • polyethylene glycol smear 0.2-10kD BCC 15 w/v -
  • magnesium formate BCC 0.2 M -
  • sodium bicine BCC 0.1 M pH 7.4
pH 7.4
200.0 μl of magnesium formate BCC (1 M)
300.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
100.0 μl of sodium bicine BCC pH 7.4 (1 M)
400.0 μl of water (100 v/v)
H6
  • polyethylene glycol smear 0.2-10kD BCC 20 w/v -
  • 2-propanol BCC 15 v/v -
  • ammonium acetate BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
187.5 μl of 2-propanol BCC (80 v/v)
40.0 μl of ammonium acetate BCC (5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
400.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
272.5 μl of water (100 v/v)
H7
  • polyethylene glycol smear 0.2-10kD BCC 20 w/v -
  • tert-butanol BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
100.0 μl of tert-butanol BCC (100 v/v)
400.0 μl of water (100 v/v)
H8
  • polyethylene glycol smear 0.2-10kD BCC 24 w/v -
  • calcium chloride BCC 0.3 M -
  • sodium bicine BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of calcium chloride BCC (1 M)
480.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
40.7 μl of sodium bicine BCC pH 7.4 (1 M)
59.3 μl of sodium bicine BCC pH 9.4 (1 M)
120.0 μl of water (100 v/v)
H9
  • polyethylene glycol smear 0.2-10kD BCC 25 w/v -
  • sodium nitrate BCC 0.3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
500.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
300.0 μl of sodium nitrate BCC (1 M)
100.0 μl of water (100 v/v)
H10
  • polyethylene glycol smear 0.2-10kD BCC 30 w/v -
NA
600.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
400.0 μl of water (100 v/v)
H11
  • polyethylene glycol smear 0.2-10kD BCC 30 w/v -
  • sodium nitrate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
600.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
200.0 μl of sodium nitrate BCC (1 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
100.0 μl of water (100 v/v)
H12
  • polyethylene glycol smear 0.2-10kD BCC 35 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
700.0 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
200.0 μl of water (100 v/v)