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Supplemental Information

Screen Description

c3_4

Created 2025-03-07.
Peggy: Mid-High MW, diverse buffers, pH range and salts.

Export

Barcodes

c3_4

  • Z0002642
  • Z0002788
  • Z0002789

Screen Structure

c3_4

Well Count 96
Number of distinct ingredients 49
Internal diversity (score mean dist) 0.930

Screen Composition

2025-06-14T13:42:26.335839 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type c3_4
Buffer 90
Complex ingredient 1
Cryoprotectant 1
Detergent 2
Organic (non-volatile) 3
Organic (volatile) 6
Polymer 96
Salt 54

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_4 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • 2-propanol BCC 10 v/v -
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium cacodylate BCC 0.1 M pH 6
pH 6
200 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
125 μl of 2-propanol BCC (80 v/v)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
575 μl of water (100 v/v)
A2
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7
  • tacsimate BCC 5 v/v pH 7
NA
1 μl of tacsimate BCC pH 4 (100 v/v)
200 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
49 μl of tacsimate BCC pH 7 (100 v/v)
650 μl of water (100 v/v)
A3
  • polyethylene glycol monomethyl ether 5000 BCC 12 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
240 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
660 μl of water (100 v/v)
A4
  • polyethylene glycol monomethyl ether 5000 BCC 15 w/v -
  • cesium chloride BCC 0.2 M -
NA
300 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
200 μl of cesium chloride BCC (1 M)
500 μl of water (100 v/v)
A5
  • polyethylene glycol monomethyl ether 5000 BCC 18 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
360 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
540 μl of water (100 v/v)
A6
  • polyethylene glycol monomethyl ether 5000 BCC 18 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
10 μl of tris chloride BCC pH 7 (1 M)
75 μl of potassium thiocyanate BCC (2 M)
360 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
39 μl of tris chloride BCC pH 9 (1 M)
515 μl of water (100 v/v)
A7
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • tergitol NP-40 0.01 w/v -
NA
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
10 μl of tergitol NP-40 (1 w/v)
590 μl of water (100 v/v)
A8
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of water (100 v/v)
A9
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of water (100 v/v)
A10
  • polyethylene glycol monomethyl ether 5000 BCC 24 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
480 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
420 μl of water (100 v/v)
A11
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
200 μl of ammonium sulfate BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
600 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
100 μl of water (100 v/v)
A12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
600 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
100 μl of water (100 v/v)
B1
  • polyethylene glycol monomethyl ether 5000 BCC 36 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5.5
pH 5.5
720 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
8 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
91 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
180 μl of water (100 v/v)
B2
  • CHES BCC 0.1 M pH 9
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 25 w/v -
pH 9
500 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
200 μl of magnesium formate BCC (1 M)
37 μl of CHES BCC pH 10 (0.8 M)
87 μl of CHES BCC pH 8.3 (0.8 M)
175 μl of water (100 v/v)
B3
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
500 μl of water (100 v/v)
B4
  • lithium acetate BCC 0.2 M -
  • polyethylene glycol monomethyl ether 5000 BCC 30 w/v -
  • bis-tris chloride BCC 0.1 M pH 6
pH 6
600 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
80 μl of lithium acetate BCC (2.5 M)
62 μl of bis-tris chloride BCC pH 5.5 (1 M)
37 μl of bis-tris chloride BCC pH 6.5 (1 M)
220 μl of water (100 v/v)
B5
  • magnesium acetate BCC 0.2 M -
  • polyethylene glycol 6000 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
80 μl of magnesium acetate BCC (2.5 M)
500 μl of polyethylene glycol 6000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
320 μl of water (100 v/v)
B6
  • CHES BCC 0.1 M pH 9
  • polyethylene glycol 6000 BCC 20 w/v -
pH 9
400 μl of polyethylene glycol 6000 BCC (50 w/v)
37 μl of CHES BCC pH 10 (0.8 M)
87 μl of CHES BCC pH 8.3 (0.8 M)
475 μl of water (100 v/v)
B7
  • polyethylene glycol 6000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
160 μl of polyethylene glycol 6000 BCC (50 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
740 μl of water (100 v/v)
B8
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
200 μl of polyethylene glycol 6000 BCC (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of sodium chloride BCC (2 M)
650 μl of water (100 v/v)
B9
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200 μl of polyethylene glycol 6000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
650 μl of water (100 v/v)
B10
  • calcium acetate BCC 0.1 M -
  • polyethylene glycol 6000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200 μl of polyethylene glycol 6000 BCC (50 w/v)
100 μl of calcium acetate BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
600 μl of water (100 v/v)
B11
  • polyethylene glycol 6000 BCC 15 w/v -
  • n-octyl-b-D-Glucopyranoside BCC 0.1 w/v -
NA
300 μl of polyethylene glycol 6000 BCC (50 w/v)
10 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v)
690 μl of water (100 v/v)
B12
  • polyethylene glycol 6000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
300 μl of polyethylene glycol 6000 BCC (50 w/v)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
600 μl of water (100 v/v)
C1
  • polyethylene glycol 6000 BCC 15 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
300 μl of polyethylene glycol 6000 BCC (50 w/v)
54 μl of tris chloride BCC pH 7 (1 M)
45 μl of tris chloride BCC pH 9 (1 M)
600 μl of water (100 v/v)
C2
  • polyethylene glycol 6000 BCC 15 w/v -
  • potassium thiocyanate BCC 0.15 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
300 μl of polyethylene glycol 6000 BCC (50 w/v)
10 μl of tris chloride BCC pH 7 (1 M)
75 μl of potassium thiocyanate BCC (2 M)
39 μl of tris chloride BCC pH 9 (1 M)
575 μl of water (100 v/v)
C3
  • dioxane BCC 1 v/v -
  • polyethylene glycol 6000 BCC 20 w/v -
NA
400 μl of polyethylene glycol 6000 BCC (50 w/v)
10 μl of dioxane BCC (100 v/v)
590 μl of water (100 v/v)
C4
  • polyethylene glycol 6000 BCC 20 w/v -
  • sodium chloride BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
400 μl of polyethylene glycol 6000 BCC (50 w/v)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of sodium chloride BCC (2 M)
C5
  • polyethylene glycol 6000 BCC 20 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • tert-butanol BCC 2.5 v/v -
pH 5.5
400 μl of polyethylene glycol 6000 BCC (50 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
25 μl of tert-butanol BCC (100 v/v)
475 μl of water (100 v/v)
C6
  • ADA BCC 0.1 M pH 6.5
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 6000 BCC 25 w/v -
pH 6.5
500 μl of polyethylene glycol 6000 BCC (50 w/v)
90 μl of ADA BCC pH 7.5 (0.5 M)
200 μl of lithium sulfate BCC (1 M)
109 μl of ADA BCC pH 5.5 (0.5 M)
100 μl of water (100 v/v)
C7
  • polyethylene glycol 6000 BCC 25 w/v -
  • triethanolamine hydrochloride BCC 0.1 M pH 7.5
pH 7.5
500 μl of polyethylene glycol 6000 BCC (50 w/v)
100 μl of triethanolamine hydrochloride BCC pH 7.5 (1 M)
400 μl of water (100 v/v)
C8
  • polyethylene glycol 6000 BCC 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600 μl of polyethylene glycol 6000 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
C9
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol 8000 BCC 8 w/v -
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
160 μl of polyethylene glycol 8000 BCC (50 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
740 μl of water (100 v/v)
C10
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 8 w/v -
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
160 μl of polyethylene glycol 8000 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
740 μl of water (100 v/v)
C11
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 5.5
200 μl of polyethylene glycol 8000 BCC (50 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
700 μl of water (100 v/v)
C12
  • sodium MES BCC 0.1 M pH 6
  • zinc acetate BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
20 μl of zinc acetate BCC (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
680 μl of water (100 v/v)
D1
  • sodium chloride BCC 0.2 M -
  • sodium dihydrogen-dipotassium hydrogen phosphate BCC 0.1 M pH 6.2
  • polyethylene glycol 8000 BCC 10 w/v -
pH 6.2
200 μl of polyethylene glycol 8000 BCC (50 w/v)
20 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 9.2 (1 M)
80 μl of sodium dihydrogen-dipotassium hydrogen phosphate BCC pH 4.6 (1 M)
100 μl of sodium chloride BCC (2 M)
600 μl of water (100 v/v)
D2
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
500 μl of water (100 v/v)
D3
  • ethylene glycol BCC 8 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 7.5
200 μl of polyethylene glycol 8000 BCC (50 w/v)
80 μl of ethylene glycol BCC (100 v/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
620 μl of water (100 v/v)
D4
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8
200 μl of polyethylene glycol 8000 BCC (50 w/v)
100 μl of imidazole BCC pH 8 (1 M)
700 μl of water (100 v/v)
D5
  • calcium acetate BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8
200 μl of polyethylene glycol 8000 BCC (50 w/v)
100 μl of imidazole BCC pH 8 (1 M)
200 μl of calcium acetate BCC (1 M)
500 μl of water (100 v/v)
D6
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 10 w/v -
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
200 μl of polyethylene glycol 8000 BCC (50 w/v)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
650 μl of water (100 v/v)
D7
  • CHES BCC 0.1 M pH 9.5
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 10 w/v -
pH 9.5
200 μl of polyethylene glycol 8000 BCC (50 w/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
100 μl of sodium chloride BCC (2 M)
575 μl of water (100 v/v)
D8
  • calcium chloride BCC 0.02 M -
  • sodium malonate-malonic acid BCC 0.1 M pH 5.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 5.5
20 μl of calcium chloride BCC (1 M)
240 μl of polyethylene glycol 8000 BCC (50 w/v)
7 μl of sodium malonate-malonic acid BCC pH 5 (3.4 M)
22 μl of sodium malonate-malonic acid BCC pH 6 (3.4 M)
710 μl of water (100 v/v)
D9
  • sodium cacodylate BCC 0.1 M pH 6
  • sodium chloride BCC 0.1 M -
  • polyethylene glycol 8000 BCC 12 w/v -
pH 6
240 μl of polyethylene glycol 8000 BCC (50 w/v)
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
50 μl of sodium chloride BCC (2 M)
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
610 μl of water (100 v/v)
D10
  • magnesium acetate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 6.5
40 μl of magnesium acetate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
240 μl of polyethylene glycol 8000 BCC (50 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
620 μl of water (100 v/v)
D11
  • potassium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 12 w/v -
pH 7
240 μl of polyethylene glycol 8000 BCC (50 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
100 μl of potassium chloride BCC (1 M)
560 μl of water (100 v/v)
D12
  • ammonium sulfate BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 7.5
100 μl of ammonium sulfate BCC (1 M)
240 μl of polyethylene glycol 8000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
560 μl of water (100 v/v)
E1
  • tris chloride BCC 0.1 M pH 8
  • polyethylene glycol 8000 BCC 12 w/v -
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
240 μl of polyethylene glycol 8000 BCC (50 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
660 μl of water (100 v/v)
E2
  • magnesium acetate BCC 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 12 w/v -
pH 8.5
40 μl of magnesium acetate BCC (2.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
240 μl of polyethylene glycol 8000 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
620 μl of water (100 v/v)
E3
  • lithium sulfate BCC 0.5 M -
  • polyethylene glycol 8000 BCC 15 w/v -
NA
300 μl of polyethylene glycol 8000 BCC (50 w/v)
500 μl of lithium sulfate BCC (1 M)
200 μl of water (100 v/v)
E4
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 15 w/v -
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
300 μl of polyethylene glycol 8000 BCC (50 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
600 μl of water (100 v/v)
E5
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • polyethylene glycol 8000 BCC 18 w/v -
pH 5.5
360 μl of polyethylene glycol 8000 BCC (50 w/v)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
540 μl of water (100 v/v)
E6
  • calcium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 18 w/v -
pH 6.5
360 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of calcium acetate BCC (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
340 μl of water (100 v/v)
E7
  • calcium chloride BCC 0.02 M -
  • sodium MES BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 18 w/v -
pH 6.5
20 μl of calcium chloride BCC (1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
360 μl of polyethylene glycol 8000 BCC (50 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
520 μl of water (100 v/v)
E8
  • sodium cacodylate BCC 0.1 M pH 6.5
  • zinc acetate BCC 0.02 M -
  • polyethylene glycol 8000 BCC 18 w/v -
pH 6.5
360 μl of polyethylene glycol 8000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
20 μl of zinc acetate BCC (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
520 μl of water (100 v/v)
E9
  • potassium dihydrogen phosphate BCC 0.05 M -
  • polyethylene glycol 8000 BCC 20 w/v -
NA
400 μl of polyethylene glycol 8000 BCC (50 w/v)
50 μl of potassium dihydrogen phosphate BCC (1 M)
550 μl of water (100 v/v)
E10
  • phosphate-citrate BCC 0.1 M pH 4.2
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 20 w/v -
pH 4.2
400 μl of polyethylene glycol 8000 BCC (50 w/v)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
100 μl of sodium chloride BCC (2 M)
300 μl of water (100 v/v)
E11
  • calcium acetate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of calcium acetate BCC (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
300 μl of water (100 v/v)
E12
  • magnesium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 6.5
80 μl of magnesium acetate BCC (2.5 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
420 μl of water (100 v/v)
F1
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 7.5
400 μl of polyethylene glycol 8000 BCC (50 w/v)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of water (100 v/v)
F2
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of magnesium chloride BCC (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
300 μl of water (100 v/v)
F3
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 20 w/v -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
400 μl of polyethylene glycol 8000 BCC (50 w/v)
100 μl of sodium bicine BCC pH 9.4 (1 M)
200 μl of magnesium chloride BCC (1 M)
300 μl of water (100 v/v)
F4
  • CHES BCC 0.1 M pH 9.5
  • polyethylene glycol 8000 BCC 20 w/v -
pH 9.5
400 μl of polyethylene glycol 8000 BCC (50 w/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
475 μl of water (100 v/v)
F5
  • CAPS BCC 0.1 M pH 10
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 20 w/v -
pH 10
114 μl of CAPS BCC pH 11 (0.5 M)
400 μl of polyethylene glycol 8000 BCC (50 w/v)
86 μl of CAPS BCC pH 9.4 (0.5 M)
100 μl of sodium chloride BCC (2 M)
300 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 8000 BCC 30 w/v -
NA
200 μl of ammonium sulfate BCC (1 M)
600 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of water (100 v/v)
F7
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 8000 BCC 30 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
600 μl of polyethylene glycol 8000 BCC (50 w/v)
200 μl of lithium sulfate BCC (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
100 μl of water (100 v/v)
F8
  • ammonium sulfate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
200 μl of ammonium sulfate BCC (1 M)
600 μl of polyethylene glycol 8000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
100 μl of water (100 v/v)
F9
  • sodium acetate BCC 0.2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • polyethylene glycol 8000 BCC 30 w/v -
pH 6.5
600 μl of polyethylene glycol 8000 BCC (50 w/v)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
200 μl of sodium acetate BCC (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
100 μl of water (100 v/v)
F10
  • imidazole BCC 0.1 M pH 8
  • sodium chloride BCC 0.2 M -
  • polyethylene glycol 8000 BCC 30 w/v -
pH 8
600 μl of polyethylene glycol 8000 BCC (50 w/v)
100 μl of imidazole BCC pH 8 (1 M)
100 μl of sodium chloride BCC (2 M)
200 μl of water (100 v/v)
F11
  • DL-malate-imidazole BCC 0.2 M pH 8
  • polyethylene glycol 10000 BCC 7.5 w/v -
pH 8
8 μl of DL-malate-imidazole BCC pH 2 (1 M)
250 μl of polyethylene glycol 10000 BCC (30 w/v)
192 μl of DL-malate-imidazole BCC pH 9 (1 M)
550 μl of water (100 v/v)
F12
  • polyethylene glycol 10000 BCC 9 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
300 μl of polyethylene glycol 10000 BCC (30 w/v)
600 μl of water (100 v/v)
G1
  • DL-malate-imidazole BCC 0.2 M pH 8
  • polyethylene glycol 10000 BCC 12.5 w/v -
pH 8
8 μl of DL-malate-imidazole BCC pH 2 (1 M)
416 μl of polyethylene glycol 10000 BCC (30 w/v)
192 μl of DL-malate-imidazole BCC pH 9 (1 M)
383 μl of water (100 v/v)
G2
  • polyethylene glycol 10000 BCC 15 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
500 μl of polyethylene glycol 10000 BCC (30 w/v)
400 μl of water (100 v/v)
G3
  • ammonium acetate BCC 0.1 M -
  • polyethylene glycol 10000 BCC 17 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
566 μl of polyethylene glycol 10000 BCC (30 w/v)
20 μl of ammonium acetate BCC (5 M)
313 μl of water (100 v/v)
G4
  • DL-malate-imidazole BCC 0.2 M pH 8
  • polyethylene glycol 10000 BCC 17.5 w/v -
pH 8
8 μl of DL-malate-imidazole BCC pH 2 (1 M)
583 μl of polyethylene glycol 10000 BCC (30 w/v)
192 μl of DL-malate-imidazole BCC pH 9 (1 M)
216 μl of water (100 v/v)
G5
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyethylene glycol 10000 BCC 20 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
666 μl of polyethylene glycol 10000 BCC (30 w/v)
233 μl of water (100 v/v)
G6
  • DL-malate-imidazole BCC 0.2 M pH 8
  • polyethylene glycol 10000 BCC 22.5 w/v -
pH 8
8 μl of DL-malate-imidazole BCC pH 2 (1 M)
750 μl of polyethylene glycol 10000 BCC (30 w/v)
192 μl of DL-malate-imidazole BCC pH 9 (1 M)
50 μl of water (100 v/v)
G7
  • polyethylene glycol 10000 BCC 23 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
766 μl of polyethylene glycol 10000 BCC (30 w/v)
133 μl of water (100 v/v)
G8
  • polyethylene glycol 10000 BCC 27 w/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 4.5
pH 4.5
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
15 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
900 μl of polyethylene glycol 10000 BCC (30 w/v)
50 μl of water (100 v/v)
G9
  • polyethylene glycol 20000 BCC 8 w/v -
  • sodium MES BCC 0.1 M pH 6
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
320 μl of polyethylene glycol 20000 BCC (25 w/v)
61 μl of sodium MES BCC pH 5.2 (1 M)
580 μl of water (100 v/v)
G10
  • dioxane BCC 2 v/v -
  • polyethylene glycol 20000 BCC 10 w/v -
  • sodium bicine BCC 0.1 M pH 9
pH 9
20 μl of dioxane BCC (100 v/v)
12 μl of sodium bicine BCC pH 7.4 (1 M)
400 μl of polyethylene glycol 20000 BCC (25 w/v)
87 μl of sodium bicine BCC pH 9.4 (1 M)
480 μl of water (100 v/v)
G11
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
420 μl of water (100 v/v)
G12
  • calcium chloride BCC 0.1 M -
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
100 μl of calcium chloride BCC (1 M)
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
320 μl of water (100 v/v)
H1
  • polyethylene glycol 20000 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
420 μl of water (100 v/v)
H2
  • polyethylene glycol 20000 BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
54 μl of tris chloride BCC pH 7 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
45 μl of tris chloride BCC pH 9 (1 M)
420 μl of water (100 v/v)
H3
  • polyethylene glycol 20000 BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
21 μl of tris chloride BCC pH 7 (1 M)
480 μl of polyethylene glycol 20000 BCC (25 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
420 μl of water (100 v/v)
H4
  • sodium MES BCC 0.1 M pH 6
  • sodium bromide BCC 0.3 M -
  • polyethylene glycol smear 0.2-10kD BCC 15 w/v -
pH 6
38 μl of sodium MES BCC pH 7.2 (1 M)
300 μl of sodium bromide BCC (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
300 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
300 μl of water (100 v/v)
H5
  • magnesium formate BCC 0.2 M -
  • polyethylene glycol smear 0.2-10kD BCC 15 w/v -
  • sodium bicine BCC 0.1 M pH 7.4
pH 7.4
100 μl of sodium bicine BCC pH 7.4 (1 M)
200 μl of magnesium formate BCC (1 M)
300 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
400 μl of water (100 v/v)
H6
  • 2-propanol BCC 15 v/v -
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol smear 0.2-10kD BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
187 μl of 2-propanol BCC (80 v/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
400 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
40 μl of ammonium acetate BCC (5 M)
272 μl of water (100 v/v)
H7
  • sodium HEPES BCC 0.1 M pH 7.5
  • tert-butanol BCC 10 v/v -
  • polyethylene glycol smear 0.2-10kD BCC 20 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
100 μl of tert-butanol BCC (100 v/v)
400 μl of water (100 v/v)
H8
  • calcium chloride BCC 0.3 M -
  • polyethylene glycol smear 0.2-10kD BCC 24 w/v -
  • sodium bicine BCC 0.1 M pH 8.5
pH 8.5
300 μl of calcium chloride BCC (1 M)
40 μl of sodium bicine BCC pH 7.4 (1 M)
59 μl of sodium bicine BCC pH 9.4 (1 M)
480 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
120 μl of water (100 v/v)
H9
  • sodium nitrate BCC 0.3 M -
  • polyethylene glycol smear 0.2-10kD BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
300 μl of sodium nitrate BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
100 μl of water (100 v/v)
H10
  • polyethylene glycol smear 0.2-10kD BCC 30 w/v -
NA
600 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
400 μl of water (100 v/v)
H11
  • sodium nitrate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • polyethylene glycol smear 0.2-10kD BCC 30 w/v -
pH 7
100 μl of tris chloride BCC pH 7 (1 M)
200 μl of sodium nitrate BCC (1 M)
600 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
100 μl of water (100 v/v)
H12
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • polyethylene glycol smear 0.2-10kD BCC 35 w/v -
pH 5.5
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
700 μl of polyethylene glycol smear 0.2-10kD BCC (50 w/v)
200 μl of water (100 v/v)