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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Classic HTS1
Barcodes
Classic HTS1
No barcodes found.
Screen Structure
Classic HTS1
Well Count | 96 |
---|---|
Number of distinct ingredients | 38 |
Internal diversity (score mean dist) | 0.919 |
Screen Composition
ingredient type | Classic HTS1 |
---|---|
Buffer | 85 |
Cryoprotectant | 4 |
Organic (non-volatile) | 13 |
Organic (volatile) | 8 |
Polymer | 96 |
Salt | 88 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.6 | 20.0 μl of Calcium chloride (5 M) 200.0 μl of Polyethylene glycol 400 (75 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 680.0 μl of water (100 v/v) |
A2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 400 (75 w/v) 620.0 μl of water (100 v/v) |
A3 |
| pH 4.6 | 40.0 μl of Magnesium chloride (2.5 M) 333.3 μl of Polyethylene glycol 400 (75 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 526.7 μl of water (100 v/v) |
A4 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 333.3 μl of Polyethylene glycol 400 (75 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
A5 |
| pH 4.6 | 20.0 μl of Calcium chloride (5 M) 400.0 μl of Polyethylene glycol 400 (75 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 480.0 μl of water (100 v/v) |
A6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 400 (75 w/v) 40.0 μl of Sodium acetate (2.5 M) 460.0 μl of water (100 v/v) |
A7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 400 (75 w/v) 420.0 μl of water (100 v/v) |
A8 |
| pH 8.5 | 400.0 μl of Polyethylene glycol 400 (75 w/v) 50.0 μl of tri-Sodium citrate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 450.0 μl of water (100 v/v) |
A9 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 100.0 μl of Sodium chloride (1 M) 200.0 μl of water (100 v/v) |
A10 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 10.0 μl of Zinc sulfate (1 M) 390.0 μl of water (100 v/v) |
A11 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of water (100 v/v) |
A12 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
B1 |
| NA | 300.0 μl of Polyethylene glycol 1,500 (50 w/v) 700.0 μl of water (100 v/v) |
B2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
B3 |
| NA | 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
B4 |
| pH 8.5 | 10.0 μl of Nickel (II) chloride (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v) |
B5 |
| NA | 500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 420.0 μl of water (100 v/v) |
B7 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
B8 |
| pH 4.6 | 80.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 820.0 μl of water (100 v/v) |
B9 |
| NA | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 840.0 μl of water (100 v/v) |
B10 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
B11 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 620.0 μl of water (100 v/v) |
B12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 620.0 μl of water (100 v/v) |
C1 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 513.3 μl of water (100 v/v) |
C2 |
| pH 8.5 | 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
C3 |
| pH 4.6 | 360.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 540.0 μl of water (100 v/v) |
C4 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 433.3 μl of water (100 v/v) |
C5 |
| pH 8.5 | 40.0 μl of Calcium chloride (5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 460.0 μl of water (100 v/v) |
C6 |
| pH 4.6 | 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of water (100 v/v) |
C7 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v) |
C8 |
| pH 8.5 | 40.0 μl of Calcium chloride (5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 360.0 μl of water (100 v/v) |
C9 |
| NA | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
C10 |
| pH 4.6 | 40.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 260.0 μl of water (100 v/v) |
C11 |
| pH 7.5 | 40.0 μl of Calcium chloride (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 260.0 μl of water (100 v/v) |
C12 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
D1 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
D2 |
| NA | 700.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v) |
D3 |
| pH 8.5 | 160.0 μl of Lithium chloride (5 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 580.0 μl of water (100 v/v) |
D4 |
| NA | 400.0 μl of 2-Propanol (50 w/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
D5 |
| pH 5.6 | 200.0 μl of 2-Propanol (50 w/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 575.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 400.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v) |
D7 |
| pH 4.6 | 66.7 μl of Ammonium sulfate (3 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 593.3 μl of water (100 v/v) |
D8 |
| pH 5.6 | 66.7 μl of Ammonium sulfate (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 608.3 μl of water (100 v/v) |
D9 |
| pH 7.5 | 200.0 μl of 2-Propanol (50 w/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 313.3 μl of water (100 v/v) |
D10 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 533.3 μl of water (100 v/v) |
D11 |
| NA | 125.0 μl of Glycerol (80 w/v) 200.0 μl of Magnesium sulfate (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 275.0 μl of water (100 v/v) |
D12 |
| NA | 400.0 μl of 2-Propanol (50 w/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate (4 M) 175.0 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 120.0 μl of Sodium chloride (5 M) 380.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 200.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v) |
E3 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 453.3 μl of water (100 v/v) |
E4 |
| pH 5.6 | 66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 408.3 μl of water (100 v/v) |
E5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 66.7 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 293.3 μl of water (100 v/v) |
E6 |
| NA | 160.0 μl of 2-Propanol (50 w/v) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 300.0 μl of water (100 v/v) |
E7 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 333.3 μl of water (100 v/v) |
E8 |
| pH 5.6 | 33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 341.7 μl of water (100 v/v) |
E9 |
| pH 8.5 | 160.0 μl of Lithium chloride (5 M) 640.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v) |
E10 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v) |
E11 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v) |
E12 |
| pH 8.5 | 60.0 μl of Polyethylene glycol 6,000 (50 w/v) 20.0 μl of Potassium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 820.0 μl of water (100 v/v) |
F1 |
| NA | 4.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 796.0 μl of water (100 v/v) |
F2 |
| NA | 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
F3 |
| NA | 62.5 μl of Glycerol (80 w/v) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 637.5 μl of water (100 v/v) |
F4 |
| NA | 4.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 10.0 μl of Potassium chloride (5 M) 686.0 μl of water (100 v/v) |
F5 |
| pH 8 | 50.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 550.0 μl of water (100 v/v) |
F6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Lithium chloride (5 M) 500.0 μl of Polyethylene glycol 6,000 (50 w/v) 380.0 μl of water (100 v/v) |
F7 |
| pH 8.5 | 100.0 μl of Lithium chloride (5 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 240.0 μl of water (100 v/v) |
F8 |
| NA | 200.0 μl of Lithium chloride (5 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 160.0 μl of water (100 v/v) |
F9 |
| NA | 166.7 μl of Lithium sulfate (3 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 793.3 μl of water (100 v/v) |
F10 |
| NA | 333.3 μl of Lithium sulfate (3 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 626.7 μl of water (100 v/v) |
F11 |
| NA | 80.0 μl of Polyethylene glycol 8,000 (50 w/v) 920.0 μl of water (100 v/v) |
F12 |
| NA | 40.0 μl of Lithium chloride (5 M) 50.0 μl of Magnesium sulfate (1 M) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 750.0 μl of water (100 v/v) |
G1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Zinc acetate (2.5 M) 620.0 μl of water (100 v/v) |
G2 |
| pH 7.5 | 100.0 μl of Calcium acetate (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
G3 |
| NA | 25.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 735.0 μl of water (100 v/v) |
G4 |
| NA | 100.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v) |
G5 |
| pH 7.5 | 200.0 μl of Ethylene glycol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
G6 |
| NA | 125.0 μl of Glycerol (80 w/v) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Potassium chloride (5 M) 535.0 μl of water (100 v/v) |
G7 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 633.3 μl of water (100 v/v) |
G8 |
| NA | 166.7 μl of Lithium sulfate (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 533.3 μl of water (100 v/v) |
G9 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 520.0 μl of water (100 v/v) |
G10 |
| pH 7.5 | 100.0 μl of Calcium acetate (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 440.0 μl of water (100 v/v) |
G11 |
| pH 7.5 | 40.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 460.0 μl of water (100 v/v) |
G12 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 473.3 μl of water (100 v/v) |
H1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v) |
H2 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H3 |
| NA | 40.0 μl of Lithium chloride (5 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 460.0 μl of water (100 v/v) |
H4 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 333.3 μl of water (100 v/v) |
H5 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
H6 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 280.0 μl of Polyethylene glycol 10,000 (50 w/v) 620.0 μl of water (100 v/v) |
H7 |
| pH 8.5 | 400.0 μl of Glycerol (50 w/v) 360.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 40.0 μl of water (100 v/v) |
H8 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v) |
H9 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
H10 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 700.0 μl of water (100 v/v) |
H11 |
| pH 8.5 | 40.0 μl of Magnesium chloride (2.5 M) 340.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v) |
H12 |
| NA | 400.0 μl of Polyethylene glycol 20,000 (50 w/v) 600.0 μl of water (100 v/v) |