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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Classic HTS1
Barcodes
Classic HTS1
Screen Structure
Classic HTS1
Well Count | 96 |
---|---|
Number of distinct ingredients | 38 |
Internal diversity (score mean dist) | 0.919 |
Screen Composition
ingredient type | Classic HTS1 |
---|---|
Buffer | 85 |
Cryoprotectant | 4 |
Organic (non-volatile) | 13 |
Organic (volatile) | 8 |
Polymer | 96 |
Salt | 88 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Calcium chloride (5 M) 200 μl of Polyethylene glycol 400 (75 w/v) 680 μl of water (100 v/v) |
A2 |
| pH 7.5 | 80 μl of Magnesium chloride (2.5 M) 200 μl of Polyethylene glycol 400 (75 w/v) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v) |
A3 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 40 μl of Magnesium chloride (2.5 M) 333 μl of Polyethylene glycol 400 (75 w/v) 526 μl of water (100 v/v) |
A4 |
| pH 8.5 | 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 333 μl of Polyethylene glycol 400 (75 w/v) 500 μl of water (100 v/v) |
A5 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Calcium chloride (5 M) 400 μl of Polyethylene glycol 400 (75 w/v) 480 μl of water (100 v/v) |
A6 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 40 μl of Sodium acetate (2.5 M) 400 μl of Polyethylene glycol 400 (75 w/v) 460 μl of water (100 v/v) |
A7 |
| pH 7.5 | 80 μl of Magnesium chloride (2.5 M) 400 μl of Polyethylene glycol 400 (75 w/v) 100 μl of HEPES pH 7.5 (1 M) 420 μl of water (100 v/v) |
A8 |
| pH 8.5 | 50 μl of tri-Sodium citrate (4 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 400 (75 w/v) 450 μl of water (100 v/v) |
A9 |
| pH 9 | 100 μl of BICINE pH 9 (1 M) 100 μl of Sodium chloride (1 M) 600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 200 μl of water (100 v/v) |
A10 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 500 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 10 μl of Zinc sulfate (1 M) 390 μl of water (100 v/v) |
A11 |
| pH 7.5 | 500 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v) |
A12 |
| pH 8.5 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
B1 |
| NA | 300 μl of Polyethylene glycol 1,500 (50 w/v) 700 μl of water (100 v/v) |
B2 |
| pH 7.5 | 400 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
B3 |
| NA | 600 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v) |
B4 |
| pH 8.5 | 10 μl of Nickel (II) chloride (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 490 μl of water (100 v/v) |
B5 |
| NA | 500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500 μl of water (100 v/v) |
B6 |
| pH 7.5 | 80 μl of Sodium acetate (2.5 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 420 μl of water (100 v/v) |
B7 |
| pH 8.5 | 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 3,000 (50 w/v) 233 μl of water (100 v/v) |
B8 |
| pH 4.6 | 80 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 820 μl of water (100 v/v) |
B9 |
| NA | 160 μl of Polyethylene glycol 4,000 (50 w/v) 840 μl of water (100 v/v) |
B10 |
| pH 4.6 | 160 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 740 μl of water (100 v/v) |
B11 |
| pH 6.5 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 620 μl of water (100 v/v) |
B12 |
| pH 7.5 | 240 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Sodium acetate (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v) |
C1 |
| pH 8.5 | 320 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 513 μl of water (100 v/v) |
C2 |
| pH 8.5 | 320 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Sodium acetate (2.5 M) 500 μl of water (100 v/v) |
C3 |
| pH 4.6 | 360 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 540 μl of water (100 v/v) |
C4 |
| pH 8.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 433 μl of water (100 v/v) |
C5 |
| pH 8.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Calcium chloride (5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 460 μl of water (100 v/v) |
C6 |
| pH 4.6 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of water (100 v/v) |
C7 |
| pH 6.5 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
C8 |
| pH 8.5 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Calcium chloride (5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 360 μl of water (100 v/v) |
C9 |
| NA | 600 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of water (100 v/v) |
C10 |
| pH 4.6 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 40 μl of Magnesium chloride (2.5 M) 260 μl of water (100 v/v) |
C11 |
| pH 7.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Calcium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 260 μl of water (100 v/v) |
C12 |
| pH 8.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Sodium acetate (2.5 M) 220 μl of water (100 v/v) |
D1 |
| pH 8.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 220 μl of water (100 v/v) |
D2 |
| NA | 700 μl of Polyethylene glycol 4,000 (50 w/v) 300 μl of water (100 v/v) |
D3 |
| pH 8.5 | 160 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 160 μl of Lithium chloride (5 M) 580 μl of water (100 v/v) |
D4 |
| NA | 200 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of 2-Propanol (50 w/v) 400 μl of water (100 v/v) |
D5 |
| pH 5.6 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (50 w/v) 25 μl of tri-Sodium citrate pH 5.6 (4 M) 575 μl of water (100 v/v) |
D6 |
| pH 7.5 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of 2-Propanol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |
D7 |
| pH 4.6 | 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 66 μl of Ammonium sulfate (3 M) 593 μl of water (100 v/v) |
D8 |
| pH 5.6 | 300 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of tri-Sodium citrate pH 5.6 (4 M) 66 μl of Ammonium sulfate (3 M) 608 μl of water (100 v/v) |
D9 |
| pH 7.5 | 320 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 66 μl of Ammonium sulfate (3 M) 313 μl of water (100 v/v) |
D10 |
| NA | 400 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 533 μl of water (100 v/v) |
D11 |
| NA | 400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of Magnesium sulfate (1 M) 125 μl of Glycerol (80 w/v) 275 μl of water (100 v/v) |
D12 |
| NA | 25 μl of tri-Sodium citrate (4 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of 2-Propanol (50 w/v) 175 μl of water (100 v/v) |
E1 |
| pH 6.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 120 μl of Sodium chloride (5 M) 380 μl of water (100 v/v) |
E2 |
| pH 7.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |
E3 |
| NA | 440 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Sodium acetate (2.5 M) 66 μl of Ammonium sulfate (3 M) 453 μl of water (100 v/v) |
E4 |
| pH 5.6 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of tri-Sodium citrate pH 5.6 (4 M) 66 μl of Ammonium sulfate (3 M) 408 μl of water (100 v/v) |
E5 |
| pH 7.5 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 40 μl of Sodium acetate (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 293 μl of water (100 v/v) |
E6 |
| NA | 500 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Sodium acetate (2.5 M) 160 μl of 2-Propanol (50 w/v) 300 μl of water (100 v/v) |
E7 |
| NA | 600 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 333 μl of water (100 v/v) |
E8 |
| pH 5.6 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of tri-Sodium citrate pH 5.6 (4 M) 33 μl of Ammonium sulfate (3 M) 341 μl of water (100 v/v) |
E9 |
| pH 8.5 | 640 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 160 μl of Lithium chloride (5 M) 100 μl of water (100 v/v) |
E10 |
| pH 8.5 | 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333 μl of water (100 v/v) |
E11 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v) |
E12 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 60 μl of Polyethylene glycol 6,000 (50 w/v) 20 μl of Potassium chloride (5 M) 820 μl of water (100 v/v) |
F1 |
| NA | 4 μl of Magnesium chloride (2.5 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 796 μl of water (100 v/v) |
F2 |
| NA | 400 μl of Sodium chloride (5 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 360 μl of water (100 v/v) |
F3 |
| NA | 62 μl of Glycerol (80 w/v) 300 μl of Polyethylene glycol 6,000 (50 w/v) 637 μl of water (100 v/v) |
F4 |
| NA | 4 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 6,000 (50 w/v) 10 μl of Potassium chloride (5 M) 686 μl of water (100 v/v) |
F5 |
| pH 8 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 50 μl of Imidazole pH 8 (1 M) 550 μl of water (100 v/v) |
F6 |
| pH 7.5 | 500 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 20 μl of Lithium chloride (5 M) 380 μl of water (100 v/v) |
F7 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 560 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Lithium chloride (5 M) 240 μl of water (100 v/v) |
F8 |
| NA | 600 μl of Polyethylene glycol 6,000 (50 w/v) 40 μl of Sodium acetate (2.5 M) 200 μl of Lithium chloride (5 M) 160 μl of water (100 v/v) |
F9 |
| NA | 166 μl of Lithium sulfate (3 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 793 μl of water (100 v/v) |
F10 |
| NA | 333 μl of Lithium sulfate (3 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 626 μl of water (100 v/v) |
F11 |
| NA | 80 μl of Polyethylene glycol 8,000 (50 w/v) 920 μl of water (100 v/v) |
F12 |
| NA | 50 μl of Magnesium sulfate (1 M) 160 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Lithium chloride (5 M) 750 μl of water (100 v/v) |
G1 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Zinc acetate (2.5 M) 620 μl of water (100 v/v) |
G2 |
| pH 7.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Calcium acetate (2 M) 600 μl of water (100 v/v) |
G3 |
| NA | 40 μl of Sodium acetate (2.5 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 25 μl of Magnesium acetate (2 M) 735 μl of water (100 v/v) |
G4 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium acetate (2 M) 700 μl of water (100 v/v) |
G5 |
| pH 7.5 | 200 μl of Ethylene glycol (50 w/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
G6 |
| NA | 125 μl of Glycerol (80 w/v) 240 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Potassium chloride (5 M) 535 μl of water (100 v/v) |
G7 |
| NA | 300 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 633 μl of water (100 v/v) |
G8 |
| NA | 166 μl of Lithium sulfate (3 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 533 μl of water (100 v/v) |
G9 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 80 μl of Sodium acetate (2.5 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 520 μl of water (100 v/v) |
G10 |
| pH 7.5 | 360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Calcium acetate (2 M) 440 μl of water (100 v/v) |
G11 |
| pH 7.5 | 40 μl of Sodium acetate (2.5 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of 2-Propanol (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 460 μl of water (100 v/v) |
G12 |
| pH 8.5 | 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 473 μl of water (100 v/v) |
H1 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium acetate (2 M) 400 μl of water (100 v/v) |
H2 |
| pH 9.5 | 100 μl of CHES pH 9.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v) |
H3 |
| NA | 500 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Lithium chloride (5 M) 460 μl of water (100 v/v) |
H4 |
| NA | 600 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Ammonium sulfate (3 M) 333 μl of water (100 v/v) |
H5 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 10,000 (50 w/v) 740 μl of water (100 v/v) |
H6 |
| pH 8 | 280 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of Imidazole pH 8 (1 M) 620 μl of water (100 v/v) |
H7 |
| pH 8.5 | 360 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Sodium chloride (1 M) 400 μl of Glycerol (50 w/v) 40 μl of water (100 v/v) |
H8 |
| pH 7.5 | 400 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
H9 |
| pH 8.5 | 600 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
H10 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 200 μl of Polyethylene glycol 20,000 (50 w/v) 700 μl of water (100 v/v) |
H11 |
| pH 8.5 | 40 μl of Magnesium chloride (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 340 μl of Polyethylene glycol 20,000 (50 w/v) 520 μl of water (100 v/v) |
H12 |
| NA | 400 μl of Polyethylene glycol 20,000 (50 w/v) 600 μl of water (100 v/v) |