Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Classic HTS1

Classics / Classic HTS1
Created 2009-11-16.

Export

Barcodes

Classic HTS1

No barcodes found.

Screen Structure

Classic HTS1

Well Count 96
Number of distinct ingredients 38
Internal diversity (score mean dist) 0.919

Screen Composition

2025-08-16T14:27:57.953112 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic HTS1
Buffer 85
Cryoprotectant 4
Organic (non-volatile) 13
Organic (volatile) 8
Polymer 96
Salt 88

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic HTS1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 15 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.1 M -
pH 4.6
20.0 μl of Calcium chloride (5 M)
200.0 μl of Polyethylene glycol 400 (75 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
680.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 400 15 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 400 (75 w/v)
620.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 400 25 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 0.1 M -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
333.3 μl of Polyethylene glycol 400 (75 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
526.7 μl of water (100 v/v)
A4
  • Polyethylene glycol 400 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
333.3 μl of Polyethylene glycol 400 (75 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 400 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.1 M -
pH 4.6
20.0 μl of Calcium chloride (5 M)
400.0 μl of Polyethylene glycol 400 (75 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
480.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 400 30 w/v -
  • MES 0.1 M pH 6.5
  • Sodium acetate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 400 (75 w/v)
40.0 μl of Sodium acetate (2.5 M)
460.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 400 30 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 400 (75 w/v)
420.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 400 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • tri-Sodium citrate 0.2 M -
pH 8.5
400.0 μl of Polyethylene glycol 400 (75 w/v)
50.0 μl of tri-Sodium citrate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
450.0 μl of water (100 v/v)
A9
  • Polyethylene glycol monomethyl ether 550 30 w/v -
  • BICINE 0.1 M pH 9
  • Sodium chloride 0.1 M -
pH 9
100.0 μl of BICINE pH 9 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
100.0 μl of Sodium chloride (1 M)
200.0 μl of water (100 v/v)
A10
  • Polyethylene glycol monomethyl ether 550 25 w/v -
  • MES 0.1 M pH 6.5
  • Zinc sulfate 0.01 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
10.0 μl of Zinc sulfate (1 M)
390.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 1,000 25 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 1,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 1,500 15 w/v -
NA
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
700.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 1,500 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
B4
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Nickel (II) chloride 0.01 M -
pH 8.5
10.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
B5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,000 20 w/v -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
420.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B8
  • Polyethylene glycol 4,000 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
820.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 4,000 8 w/v -
NA
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
840.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 4,000 10 w/v -
  • MES 0.1 M pH 6.5
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
620.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 4,000 12 w/v -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
620.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 4,000 16 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
513.3 μl of water (100 v/v)
C2
  • Polyethylene glycol 4,000 16 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.2 M -
pH 8.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 4,000 18 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
540.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 4,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
433.3 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 0.2 M -
pH 8.5
40.0 μl of Calcium chloride (5 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
460.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 4,000 25 w/v -
  • MES 0.1 M pH 6.5
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
320.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 4,000 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 0.2 M -
pH 8.5
40.0 μl of Calcium chloride (5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 4,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 0.1 M -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
260.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 4,000 30 w/v -
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 0.2 M -
pH 7.5
40.0 μl of Calcium chloride (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
260.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.2 M -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 4,000 35 w/v -
NA
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 4,000 8 w/v -
  • Lithium chloride 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
160.0 μl of Lithium chloride (5 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 20 w/v -
NA
400.0 μl of 2-Propanol (50 w/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 10 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
200.0 μl of 2-Propanol (50 w/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
575.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
400.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 4,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.2 M -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
593.3 μl of water (100 v/v)
D8
  • Polyethylene glycol 4,000 15 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 0.2 M -
pH 5.6
66.7 μl of Ammonium sulfate (3 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
608.3 μl of water (100 v/v)
D9
  • Polyethylene glycol 4,000 16 w/v -
  • 2-Propanol 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.2 M -
pH 7.5
200.0 μl of 2-Propanol (50 w/v)
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
313.3 μl of water (100 v/v)
D10
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
533.3 μl of water (100 v/v)
D11
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 10 w/v -
  • Magnesium sulfate 0.2 M -
NA
125.0 μl of Glycerol (80 w/v)
200.0 μl of Magnesium sulfate (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
275.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 w/v -
  • tri-Sodium citrate 0.1 M -
NA
400.0 μl of 2-Propanol (50 w/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate (4 M)
175.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 4,000 20 w/v -
  • MES 0.1 M pH 6.5
  • Sodium chloride 0.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
120.0 μl of Sodium chloride (5 M)
380.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 4,000 22 w/v -
  • Sodium acetate 0.1 M -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
453.3 μl of water (100 v/v)
E4
  • Polyethylene glycol 4,000 25 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 0.2 M -
pH 5.6
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
408.3 μl of water (100 v/v)
E5
  • Polyethylene glycol 4,000 25 w/v -
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
66.7 μl of Lithium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
293.3 μl of water (100 v/v)
E6
  • Polyethylene glycol 4,000 25 w/v -
  • 2-Propanol 8 w/v -
  • Sodium acetate 0.1 M -
NA
160.0 μl of 2-Propanol (50 w/v)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
300.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
333.3 μl of water (100 v/v)
E8
  • Polyethylene glycol 4,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 0.1 M -
pH 5.6
33.3 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
341.7 μl of water (100 v/v)
E9
  • Polyethylene glycol 4,000 32 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium chloride 0.8 M -
pH 8.5
160.0 μl of Lithium chloride (5 M)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
E10
  • Polyethylene glycol monomethyl ether 5,000 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
E11
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
233.3 μl of water (100 v/v)
E12
  • Polyethylene glycol 6,000 3 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium chloride 0.1 M -
pH 8.5
60.0 μl of Polyethylene glycol 6,000 (50 w/v)
20.0 μl of Potassium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
820.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 6,000 10 w/v -
  • Magnesium chloride 0.01 M -
NA
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
796.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 6,000 12 w/v -
  • Sodium chloride 2 M -
NA
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 6,000 15 w/v -
  • Glycerol 5 w/v -
NA
62.5 μl of Glycerol (80 w/v)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
637.5 μl of water (100 v/v)
F4
  • Polyethylene glycol 6,000 15 w/v -
  • Potassium chloride 0.05 M -
  • Magnesium chloride 0.01 M -
NA
4.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of Potassium chloride (5 M)
686.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 6,000 20 w/v -
  • Imidazole 0.05 M pH 8
pH 8
50.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
550.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 6,000 25 w/v -
  • HEPES 0.1 M pH 7.5
  • Lithium chloride 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Lithium chloride (5 M)
500.0 μl of Polyethylene glycol 6,000 (50 w/v)
380.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 6,000 28 w/v -
  • Lithium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium chloride (5 M)
560.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
240.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 6,000 30 w/v -
  • Lithium chloride 1 M -
  • Sodium acetate 0.1 M -
NA
200.0 μl of Lithium chloride (5 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
160.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 0.5 M -
NA
166.7 μl of Lithium sulfate (3 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
793.3 μl of water (100 v/v)
F10
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
NA
333.3 μl of Lithium sulfate (3 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
626.7 μl of water (100 v/v)
F11
  • Polyethylene glycol 8,000 4 w/v -
NA
80.0 μl of Polyethylene glycol 8,000 (50 w/v)
920.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 8,000 8 w/v -
  • Lithium chloride 0.2 M -
  • Magnesium sulfate 0.05 M -
NA
40.0 μl of Lithium chloride (5 M)
50.0 μl of Magnesium sulfate (1 M)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
750.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 8,000 10 w/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Zinc acetate (2.5 M)
620.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Calcium acetate 0.2 M -
pH 7.5
100.0 μl of Calcium acetate (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 8,000 10 w/v -
  • Magnesium acetate 0.05 M -
  • Sodium acetate 0.1 M -
NA
25.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
735.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 8,000 10 w/v -
  • Magnesium acetate 0.2 M -
NA
100.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Ethylene glycol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 8,000 12 w/v -
  • Glycerol 10 w/v -
  • Potassium chloride 0.5 M -
NA
125.0 μl of Glycerol (80 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium chloride (5 M)
535.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 8,000 15 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
633.3 μl of water (100 v/v)
G8
  • Polyethylene glycol 8,000 15 w/v -
  • Lithium sulfate 0.5 M -
NA
166.7 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
533.3 μl of water (100 v/v)
G9
  • Polyethylene glycol 8,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Sodium acetate 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
520.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 8,000 18 w/v -
  • HEPES 0.1 M pH 7.5
  • Calcium acetate 0.2 M -
pH 7.5
100.0 μl of Calcium acetate (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
440.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 8,000 18 w/v -
  • 2-Propanol 2 w/v -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.1 M -
pH 7.5
40.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
460.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 8,000 18 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
473.3 μl of water (100 v/v)
H1
  • Polyethylene glycol 8,000 20 w/v -
  • MES 0.1 M pH 6.5
  • Magnesium acetate 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 8,000 25 w/v -
  • Lithium chloride 0.2 M -
NA
40.0 μl of Lithium chloride (5 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
460.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
H5
  • Polyethylene glycol 10,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 10,000 14 w/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
280.0 μl of Polyethylene glycol 10,000 (50 w/v)
620.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 10,000 18 w/v -
  • Glycerol 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.1 M -
pH 8.5
400.0 μl of Glycerol (50 w/v)
360.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
40.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 10,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 10,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 20,000 10 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
700.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 20,000 17 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.1 M -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
340.0 μl of Polyethylene glycol 20,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 20,000 20 w/v -
NA
400.0 μl of Polyethylene glycol 20,000 (50 w/v)
600.0 μl of water (100 v/v)