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Supplemental Information

Screen Description

Classic HTS1

Classics / Classic HTS1
Created 2009-11-16.

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Classic HTS1

    Screen Structure

    Classic HTS1

    Well Count 96
    Number of distinct ingredients 38
    Internal diversity (score mean dist) 0.919

    Screen Composition

    2025-06-13T21:37:36.137965 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classic HTS1
    Buffer 85
    Cryoprotectant 4
    Organic (non-volatile) 13
    Organic (volatile) 8
    Polymer 96
    Salt 88

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classic HTS1 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 400 15 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Calcium chloride (5 M)
    200 μl of Polyethylene glycol 400 (75 w/v)
    680 μl of water (100 v/v)
    A2
    • Polyethylene glycol 400 15 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 400 (75 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    A3
    • Polyethylene glycol 400 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    333 μl of Polyethylene glycol 400 (75 w/v)
    526 μl of water (100 v/v)
    A4
    • Polyethylene glycol 400 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Polyethylene glycol 400 (75 w/v)
    500 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Calcium chloride (5 M)
    400 μl of Polyethylene glycol 400 (75 w/v)
    480 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 30 w/v -
    • MES 0.1 M pH 6.5
    • Sodium acetate 0.1 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    40 μl of Sodium acetate (2.5 M)
    400 μl of Polyethylene glycol 400 (75 w/v)
    460 μl of water (100 v/v)
    A7
    • Polyethylene glycol 400 30 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 400 (75 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    420 μl of water (100 v/v)
    A8
    • Polyethylene glycol 400 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 0.2 M -
    pH 8.5
    50 μl of tri-Sodium citrate (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 400 (75 w/v)
    450 μl of water (100 v/v)
    A9
    • Polyethylene glycol monomethyl ether 550 30 w/v -
    • BICINE 0.1 M pH 9
    • Sodium chloride 0.1 M -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    100 μl of Sodium chloride (1 M)
    600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    200 μl of water (100 v/v)
    A10
    • Polyethylene glycol monomethyl ether 550 25 w/v -
    • MES 0.1 M pH 6.5
    • Zinc sulfate 0.01 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    10 μl of Zinc sulfate (1 M)
    390 μl of water (100 v/v)
    A11
    • Polyethylene glycol 1,000 25 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    500 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    A12
    • Polyethylene glycol 1,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,500 15 w/v -
    NA
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    700 μl of water (100 v/v)
    B2
    • Polyethylene glycol 1,500 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    B3
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B4
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Nickel (II) chloride 0.01 M -
    pH 8.5
    10 μl of Nickel (II) chloride (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    490 μl of water (100 v/v)
    B5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    NA
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    B6
    • Polyethylene glycol 3,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.2 M -
    pH 7.5
    80 μl of Sodium acetate (2.5 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    420 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    233 μl of water (100 v/v)
    B8
    • Polyethylene glycol 4,000 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    820 μl of water (100 v/v)
    B9
    • Polyethylene glycol 4,000 8 w/v -
    NA
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    840 μl of water (100 v/v)
    B10
    • Polyethylene glycol 4,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    B11
    • Polyethylene glycol 4,000 10 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    pH 6.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    620 μl of water (100 v/v)
    B12
    • Polyethylene glycol 4,000 12 w/v -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.1 M -
    pH 7.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium acetate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    C1
    • Polyethylene glycol 4,000 16 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    513 μl of water (100 v/v)
    C2
    • Polyethylene glycol 4,000 16 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 0.2 M -
    pH 8.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Sodium acetate (2.5 M)
    500 μl of water (100 v/v)
    C3
    • Polyethylene glycol 4,000 18 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    360 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    540 μl of water (100 v/v)
    C4
    • Polyethylene glycol 4,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    433 μl of water (100 v/v)
    C5
    • Polyethylene glycol 4,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Calcium chloride 0.2 M -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Calcium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    460 μl of water (100 v/v)
    C6
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    C7
    • Polyethylene glycol 4,000 25 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    pH 6.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    C8
    • Polyethylene glycol 4,000 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Calcium chloride 0.2 M -
    pH 8.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Calcium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    360 μl of water (100 v/v)
    C9
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    C10
    • Polyethylene glycol 4,000 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.1 M -
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    260 μl of water (100 v/v)
    C11
    • Polyethylene glycol 4,000 30 w/v -
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    pH 7.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Calcium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    260 μl of water (100 v/v)
    C12
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 0.2 M -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Sodium acetate (2.5 M)
    220 μl of water (100 v/v)
    D1
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    D2
    • Polyethylene glycol 4,000 35 w/v -
    NA
    700 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    D3
    • Polyethylene glycol 4,000 8 w/v -
    • Lithium chloride 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Lithium chloride (5 M)
    580 μl of water (100 v/v)
    D4
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 20 w/v -
    NA
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 10 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    pH 5.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    575 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    D7
    • Polyethylene glycol 4,000 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    pH 4.6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    593 μl of water (100 v/v)
    D8
    • Polyethylene glycol 4,000 15 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 0.2 M -
    pH 5.6
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    66 μl of Ammonium sulfate (3 M)
    608 μl of water (100 v/v)
    D9
    • Polyethylene glycol 4,000 16 w/v -
    • 2-Propanol 10 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    pH 7.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    313 μl of water (100 v/v)
    D10
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    533 μl of water (100 v/v)
    D11
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 10 w/v -
    • Magnesium sulfate 0.2 M -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Magnesium sulfate (1 M)
    125 μl of Glycerol (80 w/v)
    275 μl of water (100 v/v)
    D12
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 w/v -
    • tri-Sodium citrate 0.1 M -
    NA
    25 μl of tri-Sodium citrate (4 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    175 μl of water (100 v/v)
    E1
    • Polyethylene glycol 4,000 20 w/v -
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.6 M -
    pH 6.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    120 μl of Sodium chloride (5 M)
    380 μl of water (100 v/v)
    E2
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    E3
    • Polyethylene glycol 4,000 22 w/v -
    • Sodium acetate 0.1 M -
    • Ammonium sulfate 0.2 M -
    NA
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium acetate (2.5 M)
    66 μl of Ammonium sulfate (3 M)
    453 μl of water (100 v/v)
    E4
    • Polyethylene glycol 4,000 25 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 0.2 M -
    pH 5.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    66 μl of Ammonium sulfate (3 M)
    408 μl of water (100 v/v)
    E5
    • Polyethylene glycol 4,000 25 w/v -
    • Lithium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.1 M -
    pH 7.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Lithium sulfate (3 M)
    40 μl of Sodium acetate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    293 μl of water (100 v/v)
    E6
    • Polyethylene glycol 4,000 25 w/v -
    • 2-Propanol 8 w/v -
    • Sodium acetate 0.1 M -
    NA
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium acetate (2.5 M)
    160 μl of 2-Propanol (50 w/v)
    300 μl of water (100 v/v)
    E7
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    E8
    • Polyethylene glycol 4,000 30 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 0.1 M -
    pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    33 μl of Ammonium sulfate (3 M)
    341 μl of water (100 v/v)
    E9
    • Polyethylene glycol 4,000 32 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium chloride 0.8 M -
    pH 8.5
    640 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Lithium chloride (5 M)
    100 μl of water (100 v/v)
    E10
    • Polyethylene glycol monomethyl ether 5,000 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    333 μl of water (100 v/v)
    E11
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    E12
    • Polyethylene glycol 6,000 3 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.1 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    60 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Potassium chloride (5 M)
    820 μl of water (100 v/v)
    F1
    • Polyethylene glycol 6,000 10 w/v -
    • Magnesium chloride 0.01 M -
    NA
    4 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    796 μl of water (100 v/v)
    F2
    • Polyethylene glycol 6,000 12 w/v -
    • Sodium chloride 2 M -
    NA
    400 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    360 μl of water (100 v/v)
    F3
    • Polyethylene glycol 6,000 15 w/v -
    • Glycerol 5 w/v -
    NA
    62 μl of Glycerol (80 w/v)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    637 μl of water (100 v/v)
    F4
    • Polyethylene glycol 6,000 15 w/v -
    • Potassium chloride 0.05 M -
    • Magnesium chloride 0.01 M -
    NA
    4 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    10 μl of Potassium chloride (5 M)
    686 μl of water (100 v/v)
    F5
    • Polyethylene glycol 6,000 20 w/v -
    • Imidazole 0.05 M pH 8
    pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    50 μl of Imidazole pH 8 (1 M)
    550 μl of water (100 v/v)
    F6
    • Polyethylene glycol 6,000 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Lithium chloride 0.1 M -
    pH 7.5
    500 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Lithium chloride (5 M)
    380 μl of water (100 v/v)
    F7
    • Polyethylene glycol 6,000 28 w/v -
    • Lithium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Lithium chloride (5 M)
    240 μl of water (100 v/v)
    F8
    • Polyethylene glycol 6,000 30 w/v -
    • Lithium chloride 1 M -
    • Sodium acetate 0.1 M -
    NA
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium acetate (2.5 M)
    200 μl of Lithium chloride (5 M)
    160 μl of water (100 v/v)
    F9
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 0.5 M -
    NA
    166 μl of Lithium sulfate (3 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    793 μl of water (100 v/v)
    F10
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    NA
    333 μl of Lithium sulfate (3 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    626 μl of water (100 v/v)
    F11
    • Polyethylene glycol 8,000 4 w/v -
    NA
    80 μl of Polyethylene glycol 8,000 (50 w/v)
    920 μl of water (100 v/v)
    F12
    • Polyethylene glycol 8,000 8 w/v -
    • Lithium chloride 0.2 M -
    • Magnesium sulfate 0.05 M -
    NA
    50 μl of Magnesium sulfate (1 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of Lithium chloride (5 M)
    750 μl of water (100 v/v)
    G1
    • Polyethylene glycol 8,000 10 w/v -
    • MES 0.1 M pH 6.5
    • Zinc acetate 0.2 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Zinc acetate (2.5 M)
    620 μl of water (100 v/v)
    G2
    • Polyethylene glycol 8,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    • Calcium acetate 0.2 M -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium acetate (2 M)
    600 μl of water (100 v/v)
    G3
    • Polyethylene glycol 8,000 10 w/v -
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.1 M -
    NA
    40 μl of Sodium acetate (2.5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Magnesium acetate (2 M)
    735 μl of water (100 v/v)
    G4
    • Polyethylene glycol 8,000 10 w/v -
    • Magnesium acetate 0.2 M -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    700 μl of water (100 v/v)
    G5
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 10 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    200 μl of Ethylene glycol (50 w/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    G6
    • Polyethylene glycol 8,000 12 w/v -
    • Glycerol 10 w/v -
    • Potassium chloride 0.5 M -
    NA
    125 μl of Glycerol (80 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Potassium chloride (5 M)
    535 μl of water (100 v/v)
    G7
    • Polyethylene glycol 8,000 15 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    633 μl of water (100 v/v)
    G8
    • Polyethylene glycol 8,000 15 w/v -
    • Lithium sulfate 0.5 M -
    NA
    166 μl of Lithium sulfate (3 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    533 μl of water (100 v/v)
    G9
    • Polyethylene glycol 8,000 15 w/v -
    • MES 0.1 M pH 6.5
    • Sodium acetate 0.2 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    80 μl of Sodium acetate (2.5 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    520 μl of water (100 v/v)
    G10
    • Polyethylene glycol 8,000 18 w/v -
    • HEPES 0.1 M pH 7.5
    • Calcium acetate 0.2 M -
    pH 7.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium acetate (2 M)
    440 μl of water (100 v/v)
    G11
    • Polyethylene glycol 8,000 18 w/v -
    • 2-Propanol 2 w/v -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.1 M -
    pH 7.5
    40 μl of Sodium acetate (2.5 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    G12
    • Polyethylene glycol 8,000 18 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    473 μl of water (100 v/v)
    H1
    • Polyethylene glycol 8,000 20 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium acetate 0.2 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    400 μl of water (100 v/v)
    H2
    • Polyethylene glycol 8,000 20 w/v -
    • CHES 0.1 M pH 9.5
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    H3
    • Polyethylene glycol 8,000 25 w/v -
    • Lithium chloride 0.2 M -
    NA
    500 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of Lithium chloride (5 M)
    460 μl of water (100 v/v)
    H4
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    H5
    • Polyethylene glycol 10,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 10,000 (50 w/v)
    740 μl of water (100 v/v)
    H6
    • Polyethylene glycol 10,000 14 w/v -
    • Imidazole 0.1 M pH 8
    pH 8
    280 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Imidazole pH 8 (1 M)
    620 μl of water (100 v/v)
    H7
    • Polyethylene glycol 10,000 18 w/v -
    • Glycerol 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    pH 8.5
    360 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Sodium chloride (1 M)
    400 μl of Glycerol (50 w/v)
    40 μl of water (100 v/v)
    H8
    • Polyethylene glycol 10,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    H9
    • Polyethylene glycol 10,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    600 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H10
    • Polyethylene glycol 20,000 10 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    700 μl of water (100 v/v)
    H11
    • Polyethylene glycol 20,000 17 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.1 M -
    pH 8.5
    40 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    340 μl of Polyethylene glycol 20,000 (50 w/v)
    520 μl of water (100 v/v)
    H12
    • Polyethylene glycol 20,000 20 w/v -
    NA
    400 μl of Polyethylene glycol 20,000 (50 w/v)
    600 μl of water (100 v/v)